SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF958 FitnessBrowser__Marino:GFF958 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
28% identity, 56% coverage: 63:216/275 of query aligns to 85:236/296 of P68183

query
sites
P68183

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E190 (= E170) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A172) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G176) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P189) mutation to A: No effect.

Query Sequence

>GFF958 FitnessBrowser__Marino:GFF958
MVKSRSFSFSKVMLVLGLLFLYLPMFVLIVYSFNASRLVSVWAGFSTHWYGELFRDEQIL
SAVWMSLRIAFYSASMAVCLGTLCAFVITRFKKMRGRTLLSSMITAPLVMPEVITGLSLL
LLFVQMAQTIGWPVDRGMATIWIAHTTFCSAYVAVVVSARLQEVDRSIEEAAMDLGCTPL
KTFFVITLPVIAPALVSGWLLAFTLSLDDLVIASFVSGPGATTLPMVVFSSVRMGVSPKI
NALATLIIVAVSLIAFIAWYLMRRAEHKRLQTPDA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory