SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ga0059261_0066 FitnessBrowser__Korea:Ga0059261_0066 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7rduA Crystal structure of campylobacter jejuni keto said reductoisomerase in complex with magnesium and oxidixized and reduced NADPH
68% identity, 97% coverage: 1:328/339 of query aligns to 2:328/329 of 7rduA

query
sites
7rduA

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binding nadp nicotinamide-adenine-dinucleotide phosphate: G24 (= G23), F25 (≠ Y24), G26 (= G25), S27 (= S26), Q28 (= Q27), L47 (= L47), R48 (= R48), S51 (= S51), S53 (≠ T53), V67 (≠ N67), L80 (= L80), A81 (= A81), P82 (= P82), D83 (= D83), I85 (≠ H85), I89 (= I89), A107 (= A107), H108 (= H108), P130 (= P130), K131 (= K131), A132 (≠ G132)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G24 (= G23), F25 (≠ Y24), G26 (= G25), S27 (= S26), Q28 (= Q27), L47 (= L47), R48 (= R48), S51 (= S51), S53 (≠ T53), V67 (≠ N67), L80 (= L80), A81 (= A81), P82 (= P82), D83 (= D83), I85 (≠ H85), I89 (= I89), A107 (= A107), H108 (= H108), P130 (= P130), P133 (= P133)

7latA Campylobacter jejuni keto-acid reductoisomerase in complex with mg2+
68% identity, 97% coverage: 1:328/339 of query aligns to 2:328/329 of 7latA

query
sites
7latA

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binding magnesium ion: D191 (= D191), E195 (= E195)

C1DFH7 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Azotobacter vinelandii (strain DJ / ATCC BAA-1303) (see paper)
64% identity, 100% coverage: 1:339/339 of query aligns to 1:338/338 of C1DFH7

query
sites
C1DFH7

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D190 (= D191) binding
E226 (= E227) binding
E230 (= E231) binding

4xiyA Crystal structure of ketol-acid reductoisomerase from azotobacter (see paper)
63% identity, 97% coverage: 1:328/339 of query aligns to 1:327/328 of 4xiyA

query
sites
4xiyA

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I

I

T

N

E

E

E

Q

T

S

K

R

K

Q

E

A

M

M

K

R

R

N

V

A

L

L

A

K

D

R

I

I

Q

Q

S

D

G

G

R

E

F

Y

V

A

K

K

D

M

F

F

V

I

L

T

D

E

N

G

R

A

A

A

G

N

Q

Y

P

P

E

S

L

M

K

T

A

A

S

Y

R

R

I

R

A

N

K

A

R

A

H

H

Q

Q

I

I

E

E

Q

V

V

V

G

G

S

E

E

K

L

L

R

R

A

T

M

M

P

P

W

W

I

I

active site: K130 (= K131), D190 (= D191), E194 (= E195)
binding fe (iii) ion: D190 (= D191), E194 (= E195)
binding magnesium ion: E226 (= E227), E230 (= E231)

8cy8A Apo form cryo-em structure of campylobacter jejune ketol-acid reductoisommerase crosslinked by glutaraldehyde
65% identity, 97% coverage: 1:328/339 of query aligns to 1:311/312 of 8cy8A

query
sites
8cy8A

M

I

R

T

V

V

Y

Y

D

D

R

K

D

D

A

C

D

D

L

L

N

N

L

L

I

I

T

K

G

S

K

K

I

V

A

A

I

I

V

I

G

G

Y

F

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

A

Q

M

N

N

L

L

R

R

D

D

S

N

G

G

V

V

K

N

E

-

V

V

A

T

I

I

A

G

L

L

R

R

E

N

G

-

S

-

A

-

T

-

A

-

K

-

A

-

E

-

A

-

A

A

G

G

F

F

K

E

V

V

L

M

S

S

N

V

K

S

E

E

A

A

A

S

Q

K

W

I

A

A

D

D

V

V

L

I

M

M

I

I

L

L

A

A

P

P

D

D

E

E

H

I

Q

Q

A

A

A

D

I

I

W

F

E

N

N

V

D

E

L

I

K

K

G

P

N

N

M

L

K

S

P

E

G

G

S

K

A

A

I

I

A

A

F

F

A

A

H

H

G

G

L

F

N

N

I

I

H

H

F

Y

G

G

L

Q

I

I

E

V

A

V

P

P

A

K

D

G

I

V

D

D

V

V

I

I

M

M

I

I

A

A

P

P

K

K

G

A

P

P

G

G

H

H

T

T

V

V

R

R

S

N

E

E

Y

-

V

-

R

-

G

-

V

T

P

P

C

C

L

L

I

I

A

A

V

I

H

H

Q

Q

D

D

A

E

S

S

G

K

N

N

A

A

H

K

D

N

V

L

A

A

L

L

A

S

Y

Y

A

A

S

S

G

A

V

I

G

G

R

R

S

T

G

G

I

I

E

E

T

T

N

T

F

F

R

K

E

A

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G
|
G

E
|
E

Q

Q

A

A

V

V

L
|
L

C

C

G

G

A

L

T

S

A

A

L

L

V

I

Q

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

E

P

P

E

E

M

M

A

A

Y

Y

F

F

E
|
E

C

C

L

L

H

H

E
|
E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

M

I

Y

Y

E

Q

G

G

I

I

A

A

N

D

M

M

R

R

Y

Y

S

S

I

I

S

S

N

N

T

T

A

A

E

E

Y

Y

G

G

D

D

I

Y

K

I

T

T

G

G

P

P

R

K

I

I

T

T

E

E

T

T

K

K

E

A

M

M

K

K

R

G

V

V

L

L

A

K

D

D

I

I

Q

Q

S

N

G

G

R

V

F

F

V

A

K

K

D

D

F

F

V

I

L

L

D

E

N

R

R

R

A

A

G

G

Q

F

P

A

E

R

L

M

K

H

A

A

S

E

R

R

I

K

A

N

A

M

K

N

R

D

H

S

Q

L

I

I

E

E

Q

K

V

T

G

G

S

R

E

N

L

L

R

R

A

A

M

M

P

P

W

W

I

I

binding pentanedial: D174 (= D191), G177 (= G194), E178 (= E195), L182 (= L199), E210 (= E227), E214 (= E231)

C8WR67 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Alicyclobacillus acidocaldarius subsp. acidocaldarius (strain ATCC 27009 / DSM 446 / BCRC 14685 / JCM 5260 / KCTC 1825 / NBRC 15652 / NCIMB 11725 / NRRL B-14509 / 104-IA) (Bacillus acidocaldarius) (see paper)
63% identity, 98% coverage: 2:332/339 of query aligns to 3:331/344 of C8WR67

query
sites
C8WR67

R

K

V

I

Y

Y

D

D

R

A

D

D

A

I

D

S

L

I

N

Q

L

P

I

L

T

A

G

D

K

K

K

R

I

I

A

A

I

V

V

I

G

G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

Q

Q

N

N

L

L

R

R

D

D

S

S

G

G

V

F

K

-

E

D

V

V

A

V

I

I

A

G

L

L

R
|
R

E

P

G

G

S

S

A
x
S

T

W

A

A

K

K

K

-

A

A

E

E

A

A

A

D

G

G

F

F

K

R

V

V

L

M

S

A

N

V

K

G

E

E

A

A

A

V

Q

E

W

E

A

S

D

D

V

V

L

I

M

M

I

I

L

L

A

L

P

P

D
|
D

E
|
E

H
x
R

Q
|
Q

A

P

A

A

I

V

W

Y

E

E

N

R

D

E

L

I

K

R

G

P

N

Y

M

L

K

T

P

A

G

G

S

K

A

A

I

L

A

A

F

F

A

A

H

H

G

G

L

F

N

N

I

I

H

H

F

F

G

S

L

Q

I

I

E

Q

A

P

P

P

A

K

D

D

I

V

D

D

V

V

I

F

M

M

I

V

A

A

P

P

K

K

G

G

P

P

G
|
G

H

H

T

L

V

V

R

R

S

R

E

V

Y

Y

V

E

R

A

G

G

V

V

P

P

C

A

L

L

I

I

A

A

V

V

H

H

Q

Q

D

D

A

A

S

S

G

G

N

Q

A

A

H

K

D

D

V

L

A

A

L

L

A

A

Y

Y

A

A

S

R

G

G

V

I

G

G

G

A

G

G

R

R

S

A

G

G

I

I

I

L

E

T

T

T

N

T

F

F

R

R

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

A

L

T

S

A

A

L

L

V

I

Q

K

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

Q

P

P

E

E

M

I

A

A

Y

Y

F

F

E
|
E

C

C

L

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

M

I

Y

Y

E

E

G

G

I

L

A

E

N

Y

M

M

R

R

Y

Y

S

S

I

I

S

S

N

D

T

T

A

A

E

Q

Y

W

G

G

D

D

I

F

K

T

T

S

G

G

P

P

R

R

I

I

T

N

E

E

T

T

K

K

E

E

M

M

K

R

R

R

V

I

L

L

A

A

D

D

I

I

Q

Q

S

S

G

G

R

A

F

F

V

A

K

K

D

S

F

W

V

I

L

L

D

E

N

N

R

Q

A

A

G

N

Q

R

P

P

E

M

L

F

K

N

A

A

S

I

R

N

I

R

A

R

A

E

K

L

R

E

H

H

Q

P

I

I

E

E

Q

V

V

V

G

G

S

R

E

K

L

L

R

R

A

S

M

M

P

P

W

F

I

I

G

K

A

A

N

K

K

R

YGSQ 25:28 (= YGSQ 24:27) binding
R48 (= R48) binding ; mutation to P: Inversion of the cofactor specificity from NADPH to NADH.
S52 (≠ A52) binding ; mutation to D: Inversion of the cofactor specificity from NADPH to NADH.
DERQ 82:85 (≠ DEHQ 83:86) binding
G133 (= G134) binding
D190 (= D191) binding
E194 (= E195) binding
E226 (= E227) binding

4tskA Ketol-acid reductoisomerase from alicyclobacillus acidocaldarius (see paper)
63% identity, 98% coverage: 2:332/339 of query aligns to 2:330/333 of 4tskA

query
sites
4tskA

R

K

V

I

Y

Y

D

D

R

A

D

D

A

I

D

S

L

I

N

Q

L

P

I

L

T

A

G

D

K

K

K

R

I

I

A

A

I

V

V

I

G
|
G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

Q

Q

N

N

L

L

R

R

D

D

S

S

G

G

V

F

K

-

E

D

V

V

A

V

I

I

A

G

L
|
L

R
|
R

E

P

G

G

S

S

A
x
S

T

W

A

A

K

K

K

-

A

A

E

E

A

A

A

D

G

G

F

F

K

R

V

V

L

M

S

A

N
x
V

K

G

E

E

A

A

A

V

Q

E

W

E

A

S

D

D

V

V

L

I

M

M

I

I

L
|
L

A
x
L

P
|
P

D
|
D

E

E

H

R

Q
|
Q

A

P

A

A

I
x
V

W

Y

E

E

N

R

D

E

L

I

K

R

G

P

N

Y

M

L

K

T

P

A

G

G

S

K

A

A

I

L

A

A

F

F

A
|
A

H
|
H

G

G

L

F

N

N

I

I

H

H

F

F

G

S

L

Q

I

I

E

Q

A

P

P

P

A

K

D

D

I

V

D

D

V

V

I

F

M

M

I

V

A

A

P

P

K
|
K

G
|
G

P
|
P

G
|
G

H

H

T

L

V

V

R

R

S

R

E

V

Y

Y

V

E

R

A

G

G

V

V

P

P

C

A

L

L

I

I

A

A

V

V

H

H

Q

Q

D

D

A

A

S

S

G

G

N

Q

A

A

H

K

D

D

V

L

A

A

L

L

A

A

Y

Y

A

A

S

R

G

G

V

I

G

G

G

A

G

G

R

R

S

A

G

G

I

I

I

L

E

T

T

T

N

T

F

F

R

R

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

A

L

T

S

A

A

L

L

V

I

Q

K

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

Q

P

P

E

E

M

I

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

M

I

Y

Y

E

E

G

G

I

L

A

E

N

Y

M

M

R
|
R

Y
|
Y

S

S

I
|
I

S
|
S

N
x
D

T

T

A

A

E
x
Q

Y

W

G

G

D

D

I

F

K

T

T

S

G

G

P

P

R

R

I

I

T

N

E

E

T

T

K

K

E

E

M

M

K

R

R

R

V

I

L

L

A

A

D

D

I

I

Q

Q

S

S

G

G

R

A

F

F

V

A

K

K

D

S

F

W

V

I

L

L

D

E

N

N

R

Q

A

A

G

N

Q

R

P

P

E

M

L

F

K

N

A

A

S

I

R

N

I

R

A

R

A

E

K

L

R

E

H

H

Q

P

I

I

E

E

Q

V

V

V

G

G

S

R

E

K

L

L

R

R

A

S

M

M

P

P

W

F

I

I

G

K

A

A

N

K

K

R

active site: K129 (= K131), D189 (= D191), E193 (= E195)
binding magnesium ion: D189 (= D191), D189 (= D191), E193 (= E195), E193 (= E195), R246 (= R248), Y247 (= Y249), I249 (= I251), S250 (= S252), D251 (≠ N253), Q254 (≠ E256)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G23 (= G23), Y24 (= Y24), G25 (= G25), S26 (= S26), Q27 (= Q27), L46 (= L47), R47 (= R48), S51 (≠ A52), V65 (≠ N67), L78 (= L80), L79 (≠ A81), P80 (= P82), D81 (= D83), Q84 (= Q86), V87 (≠ I89), A105 (= A107), H106 (= H108), G130 (= G132), P131 (= P133), G132 (= G134)

8ep7C Crystal structure of the ketol-acid reductoisomerase from bacillus anthracis in complex with NADP
60% identity, 97% coverage: 1:330/339 of query aligns to 1:328/328 of 8ep7C

query
sites
8ep7C

M

M

R

K

V

T

Y

Y

D

E

R

Q

D

D

A

A

D

N

L

V

N

G

L

L

I

L

T

Q

G

G

K

K

K

T

I

V

A

A

I

V

V

I

G
|
G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

Q

A

A

Q

Q

N

N

L

L

R

R

D

D

S

S

G

G

V

V

K

-

E

E

V

V

A

V

I

V

A

G

L
x
V

R
|
R

E

P

G

G

S

K

A

-

T
x
S

A

F

K

E

K

V

A

A

E

K

A

A

A

D

G

G

F

F

K

E

V

V

L

M

S

S

N

V

K

S

E

E

A

A

A

V

Q

R

W

T

A

A

D

Q

V

V

L

V

M

Q

I

M

L
|
L

A
x
L

P
|
P

D
|
D

E

E

H
x
Q

Q
|
Q

A

A

A

H

I
x
V

W

Y

E

K

N

A

D

E

L

V

K

E

G

E

N

N

M

L

K

R

P

E

G

G

S

Q

A

M

I

L

A

L

F

F

A
x
S

H
|
H

G

G

L

F

N

N

I

I

H

H

F

F

G

G

L

Q

I

I

E

N

A

P

P

P

A

S

D

Y

I

V

D

D

V

V

I

A

M

M

I

V

A

A

P
|
P

K

K

G
x
S

P
|
P

G

G

H

H

T

L

V

V

R

R

S

R

E

V

Y

F

V

Q

R

E

G

G

G

N

G

G

V

V

P

P

C

A

L

L

I

V

A

A

V

V

H

H

Q

Q

D

D

A

A

S

T

G

G

N

T

A

A

H

L

D

H

V

V

A

A

L

L

A

A

Y

Y

A

A

S

K

G

G

V

V

G

G

G

C

G

T

R

R

S

A

G

G

I

V

I

I

E

E

T

T

N

T

F

F

R

Q

E

E

C

T

E

E

T

T

D

D

L

L

F

F

G

G

E

E

Q

Q

A

A

V

V

L

L

C

C

G

G

A

V

T

T

A

A

L

L

V

V

Q

K

A

A

G

G

F

F

E

E

T

T

L

L

V

T

E

E

A

G

G

G

Y

Y

A

R

P

P

E

E

M

I

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E

E

L

L

K

K

L

L

I

I

V

V

D

D

L

L

M

M

Y

Y

E

E

G

G

I

L

A

T

N

N

M

M

R

R

Y

H

S

S

I

I

S

S

N

D

T

T

A

A

E

E

Y

F

G

G

D

D

I

Y

K

V

T

T

G

G

P

S

R

R

I

I

I

V

T

T

E

D

E

E

T

T

K

K

E

E

M

M

K

K

R

R

V

V

L

L

A

T

D

E

I

I

Q

Q

S

Q

G

G

R

E

F

F

V

A

K

K

D

K

F

W

V

I

L

L

D

E

N

N

R

Q

A

A

G

G

Q

R

P

P

E

T

L

Y

K

N

A

A

S

M

R

K

I

K

A

A

A

E

K

Q

R

N

H

H

Q

Q

I

L

E

E

Q

K

V

V

G

G

S

E

E

E

L

L

R

R

A

E

M

M

P

S

W

W

I

I

G

H

A

A

binding nadp nicotinamide-adenine-dinucleotide phosphate: G23 (= G23), Y24 (= Y24), G25 (= G25), S26 (= S26), Q27 (= Q27), V46 (≠ L47), R47 (= R48), S51 (≠ T53), L78 (= L80), L79 (≠ A81), P80 (= P82), D81 (= D83), Q83 (≠ H85), Q84 (= Q86), V87 (≠ I89), S105 (≠ A107), H106 (= H108), P128 (= P130), S130 (≠ G132), P131 (= P133)

Q02138 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) (see paper)
61% identity, 96% coverage: 1:327/339 of query aligns to 3:327/340 of Q02138

query
sites
Q02138

M

V

R

T

V

M

Y

Y

D

E

R

D

D

D

A

V

D

E

L

V

N

S

L

A

I

L

T

A

G

G

K

K

K

Q

I

I

A

A

I

V

V

I

G

G

Y

Y

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

A

Q

Q

N

N

L

L

R

R

D

D

S

S

G

G

V

-

K

H

E

N

V

V

A

I

I

I

A

G

L
x
V

R
|
R

E

H

G

G

S
x
K

A

-

T
x
S

A

F

K

D

K

K

A

A

E

K

A

E

A

D

G

G

F

F

K

E

V

T

L

F

S

E

N

V

K

G

E

E

A

A

A

V

Q

A

W

K

A

A

D

D

V

V

L

I

M

M

I

V

L

L

A

A

P

P

D

D

E

E

H

L

Q

Q

A

Q

A

S

I

I

W

Y

E

E

N

E

D

D

L

I

K

K

G

P

N

N

M

L

K

K

P

A

G

G

S

S

A

A

I

L

A

G

F

F

A

A

H

H

G

G

L

F

N

N

I

I

H

H

F

F

G

G

L

Y

I

I

E

K

A

V

P

P

A

E

D

D

I

V

D

D

V

V

I

F

M

M

I

V

A

A

P

P

K

K

G

A

P

P

G

G

H

H

T

L

V

V

R

R

S

R

E

T

Y

Y

V

T

R

E

G

G

G

F

G

G

V

T

P

P

C

A

L

L

I

F

A

V

V

S

H

H

Q

Q

D

N

A

A

S

S

G

G

N

H

A

A

H

R

D

E

V

I

A

A

L

M

A

D

Y

W

A

A

S

K

G

G

V

I

G

G

G

C

G

A

R

R

S

V

G

G

I

I

E

E

T

T

N

T

F

F

R

K

E

E

C

T

E

E

T

E

D

D

L

L

F

F

G

G

E

E

Q

Q

A

A

V

V

L

L

C

C

G

G

A

L

T

T

A

A

L

L

V

V

Q

E

A

A

G

G

F

F

E

E

T

T

L

L

V

T

E

E

A

A

G

G

Y

Y

A

A

P

G

E

E

M

L

A

A

Y

Y

F

F

E

E

C

V

L

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

M

M

Y

Y

E

E

G

G

I

F

A

T

N

K

M

M

R

R

Y

Q

S

S

I

I

S

S

N

N

T

T

A

A

E

E

Y

F

G

G

D

D

I

Y

K

V

T

T

G

G

P

P

R

R

I

I

T

T

E

D

E

A

T

V

K

K

E

N

M

M

K

K

R

L

V

V

L

L

A

A

D

D

I

I

Q

Q

S

S

G

G

R

K

F

F

V

A

K

Q

D

D

F

F

V

V

L

D

D

D

N

F

R

K

A

A

G

G

Q

R

P

P

E

K

L

L

K

T

A

A

S

Y

R

R

I

E

A

A

K

K

R

N

H

L

Q

E

I

I

E

E

Q

K

V

I

G

G

S

A

E

E

L

L

R

R

A

K

M

A

M

M

P

P

W

F

V48 (≠ L47) mutation to L: Inversion of cofactor specificity from NADPH to NADH. Strong decrease of the affinity for NADPH and 2.5-fold increase of the affinity for NADH. 8-fold decrease of the catalytic efficiency for NADPH and 4-fold increase of the catalytic efficiency for NADH; when associated with P-49, L-52 and D-53.
R49 (= R48) mutation to P: Inversion of cofactor specificity from NADPH to NADH. Strong decrease of the affinity for NADPH and 2.5-fold increase of the affinity for NADH. 8-fold decrease of the catalytic efficiency for NADPH and 4-fold increase of the catalytic efficiency for NADH; when associated with L-48, L-52 and D-53.
K52 (≠ S51) mutation to L: Inversion of cofactor specificity from NADPH to NADH. Strong decrease of the affinity for NADPH and 2.5-fold increase of the affinity for NADH. 8-fold decrease of the catalytic efficiency for NADPH and 4-fold increase of the catalytic efficiency for NADH; when associated with L-48, P-49 and D-53.
S53 (≠ T53) mutation to D: Inversion of cofactor specificity from NADPH to NADH. Strong decrease of the affinity for NADPH and 2.5-fold increase of the affinity for NADH. 8-fold decrease of the catalytic efficiency for NADPH and 4-fold increase of the catalytic efficiency for NADH; when associated with L-48, P-49 and L-52.

6jx2B Crystal structure of ketol-acid reductoisomerase from corynebacterium glutamicum (see paper)
59% identity, 97% coverage: 1:328/339 of query aligns to 1:327/327 of 6jx2B

query
sites
6jx2B

M

I

R

E

V

L

Y

L

Y

Y

D

D

R

A

D

D

A

A

D

D

L

L

N

S

L

L

I

I

T

Q

G

G

K

R

K

K

I

V

A

A

I

I

V

V

G

G

Y

Y

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

S

Q

Q

N

N

L

L

R

R

D

D

S

S

G

G

V

V

K

-

E

E

V

V

A

V

I

I

A

G

L

L

R

R

E

E

G

G

S

S

A

K

T

S

A

A

K

E

K

K

A

A

E

K

A

E

A

A

G

G

F

F

K

E

V

V

L

K

S

T

N

T

K

A

E

E

A

A

Q

A

W

W

A
|
A

D

D

V

V

L

I

M

M

I

L

L

L

A

A

P

P

D

D

E

T

H

S

Q

Q

A

A

A

E

I

I

W

F

E

T

N

N

D

D

L

I

K

E

G

P

N

N

M

L

K
x
N

P

A

G

G

S
x
D

A

A

I

L

A

L

F

F

A

G

H

H

G

G

L

L

N

N

I

I

H

H

F

F

G

D

L

L

I

I

E

K

A

P

P

A

A

D

D

D

I

I

D

I

V

V

I

G

M

M

I

V

A

A

P

P

K

K

G

G

P

P

G

G

H

H

T

L

V

V

R

R

S

R

E

Q

Y

F

V

V

R

D

G

G

G

K

G

G

V

V

P

P

C

C

L

L

I

I

A

A

V

V

H

D

Q

Q

D

D

A

P

S

T

G

G

N

T

A

A

H

Q

D

A

V

L

A

T

L

L

A

S

Y

Y

A

A

S

A

G

A

V

I

G

G

G

A

R

R

S

A

G

G

I

V

I

I

E

P

T

T

N

T

F

F

R

E

E

A

E

E

C

T

E

V

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

A

T

T

E

A

E

L

L

V

V

Q

K

A

V

G

G

F

F

E

E

T

V

L

L

V

T

E

E

A

A

G

G

Y

Y

A

E

P

P

E

E

M

M

A

A

Y

Y

F

F

E
|
E

C

V

L

L

H

H

E
|
E

L

L

K

K

L

L

I

I

V

V

D

D

L

L

M

M

Y

F

E

E

G

G

I

I

A

S

N

N

M

M

R

N

Y

Y

S

S

I

V

S

S

N

D

T

T

A

A

E

E

Y

F

G

G

D

G

I

Y

K

L

T

S

G

G

P

P

R

R

I

V

I

I

T

D

E

A

E

D

T

T

K

K

K

S

E

R

M

M

K

K

R

D

V

I

L

L

A

T

D

D

I

I

Q

Q

S

D

G

G

R

T

F

F

V

T

K

K

D

R

F

L

V

I

L

A

D

N

N

V

R

E

A

N

G

G

Q

N

P

T

E

E

L

L

K

E

A

G

S

L

R

R

I

A

A

S

A

Y

K

N

R

N

H

H

Q

P

I

I

E

E

Q

E

V

T

G

G

S

A

E

K

L

L

R

R

A

D

M

L

M

M

P

S

W

W

I

V

binding magnesium ion: A73 (= A74), N98 (≠ K99), D101 (≠ S102), D190 (= D191), D190 (= D191), E194 (= E195), E194 (= E195), E226 (= E227), E230 (= E231)

6bulB Crystal structure of staphylococcus aureus ketol-acid reductoisomerase with hydroxyoxamate inhibitor 2
59% identity, 96% coverage: 3:328/339 of query aligns to 3:326/327 of 6bulB

query
sites
6bulB

V

V

Y

Y

D

D

R

Q

D

D

A

V

D

K

L

T

N

D

L

A

I

L

T

Q

G

G

K

K

I

I

A

A

I

V

V

V

G
|
G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

Q

Q

N

N

L

L

R

K

D

D

S

N

G

G

V

Y

K

-

E

D

V

V

A

V

I

I

A

G

L
x
I

R
|
R

E

P

G

G

S

R

A

-

T
x
S

A

F

K

D

K

K

A

A

E

K

A

E

A

D

G

G

F

F

K

D

V

V

L

F

S

P

N

V

K

A

E

E

A

A

A

V

Q

K

W

Q

A

A

D

D

V

V

L

I

M

M

I

V

L
|
L

A
x
L

P
|
P

D
|
D

E

E

H
x
I

Q
|
Q

A

G

A

D

I
x
V

W

Y

E

K

N

N

D

E

L

I

K

E

G

P

N

N

M

L

K

E

P

K

G

H

S

N

A

A

I

L

A

A

F

F

A
|
A

H
|
H

G

G

L

F

N

N

I

I

H

H

F

F

G

G

L

V

I

I

E

Q

A

P

P

P

A

A

D

D

I

V

D

D

V

V

I

F

M

L

I

V

A

A

P

P

K
|
K

G
|
G

P
|
P

G
|
G

H

H

T

L

V

V

R

R

S

R

E

T

Y

F

V

V

R

E

G

G

G

S

G

A

V

V

P

P

C

S

L

L

I

F

A

G

V

I

H

Q

Q

Q

D

D

A

A

S

S

G

G

N

Q

A

A

H

R

D

N

V

I

A

A

L

L

A

S

Y

Y

A

A

S

K

G

G

V

I

G

G

G

A

G

T

R

R

S

A

G

G

I

V

I

I

E

E

T

T

N

T

F

F

R

K

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

A

V

T

S

A

K

L

L

V

I

Q

Q

A

S

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

Q

P

P

E

E

M

L

A

A

Y

Y

F

F

E

E

C

V

L

L

H

H

E
|
E

L

M

K

K

L

L

I
|
I

V

V

D

D

L

L

M

M

Y

Y

E

E

G

G

I

M

A

E

N

N

M

V

R

R

Y

Y

S
|
S

I
|
I

S
|
S

N

N

T

T

A
|
A

E

E

Y

F

G

G

D

D

I

Y

K

V

T

S

G

G

P

P

R

R

I

V

I

I

T

T

E

P

E

D

T

V

K

K

K

E

E

N

M

M

K

K

R

A

V

V

L

L

A

T

D

D

I

I

Q

Q

S

N

G

G

R

N

F

F

V

S

K

N

D

R

F

F

V

I

L

E

D

D

N

N

R

K

A

N

G

G

Q

F

P

K

E

E

L

F

K

Y

A

K

S

L

R

R

I

E

A

E

A

Q

K

H

R

G

H

H

Q

Q

I

I

E

E

Q

K

V

V

G

G

S

R

E

E

L

L

R

R

A

E

M

M

P

P

W

F

I

I

active site: K129 (= K131), D189 (= D191), E193 (= E195)
binding {hydroxy[(1S)-1-phenylethyl]amino}(oxo)acetic acid: P131 (= P133), D189 (= D191), E193 (= E195), E229 (= E231), I233 (= I235), I249 (= I251), S250 (= S252), A253 (= A255)
binding oxo{[(1S)-1-phenylethyl]amino}acetic acid: P131 (= P133), D189 (= D191), E193 (= E195), E229 (= E231), I233 (= I235), I249 (= I251), S250 (= S252), A253 (= A255)
binding magnesium ion: D189 (= D191), E193 (= E195)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G23 (= G23), Y24 (= Y24), G25 (= G25), S26 (= S26), Q27 (= Q27), I46 (≠ L47), R47 (= R48), S51 (≠ T53), L78 (= L80), L79 (≠ A81), P80 (= P82), D81 (= D83), I83 (≠ H85), Q84 (= Q86), V87 (≠ I89), A105 (= A107), H106 (= H108), G130 (= G132), P131 (= P133), G132 (= G134), S248 (= S250), I249 (= I251), S250 (= S252)

6vo2A Crystal structure of staphylococcus aureus ketol-acid reductoisomerase in complex with mg, NADPH and inhibitor. (see paper)
59% identity, 96% coverage: 3:328/339 of query aligns to 3:326/326 of 6vo2A

query
sites
6vo2A

V

V

Y

Y

D

D

R

Q

D

D

A

V

D

K

L

T

N

D

L

A

I

L

T

Q

G

G

K

K

I

I

A

A

I

V

V

V

G
|
G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

Q

Q

N

N

L

L

R

K

D

D

S

N

G

G

V

Y

K

-

E

D

V

V

A

V

I

I

A

G

L
x
I

R
|
R

E

P

G

G

S

R

A

-

T
x
S

A

F

K

D

K

K

A

A

E

K

A

E

A

D

G

G

F

F

K

D

V

V

L

F

S

P

N

V

K

A

E

E

A

A

A

V

Q

K

W

Q

A

A

D

D

V

V

L

I

M

M

I

V

L
|
L

A
x
L

P
|
P

D
|
D

E

E

H
x
I

Q
|
Q

A

G

A

D

I
x
V

W

Y

E

K

N

N

D

E

L

I

K

E

G

P

N

N

M

L

K

E

P

K

G

H

S

N

A

A

I

L

A

A

F

F

A
|
A

H
|
H

G

G

L

F

N

N

I

I

H

H

F

F

G

G

L

V

I

I

E

Q

A

P

P

P

A

A

D

D

I

V

D

D

V

V

I

F

M

L

I

V

A

A

P

P

K

K

G
|
G

P
|
P

G
|
G

H

H

T

L

V

V

R

R

S

R

E

T

Y

F

V

V

R

E

G

G

G

S

G

A

V

V

P

P

C

S

L

L

I

F

A

G

V

I

H

Q

Q

Q

D

D

A

A

S

S

G

G

N

Q

A

A

H

R

D

N

V

I

A

A

L

L

A

S

Y

Y

A

A

S

K

G

G

V

I

G

G

G

A

G

T

R

R

S

A

G

G

I

V

I

I

E

E

T

T

N

T

F

F

R

K

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

A

V

T

S

A

K

L

L

V

I

Q

Q

A

S

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

Q

P

P

E

E

M

L

A

A

Y

Y

F

F

E

E

C

V

L

L

H

H

E
|
E

L

M

K

K

L

L

I
|
I

V

V

D

D

L

L

M

M

Y

Y

E

E

G

G

I

M

A

E

N

N

M

V

R

R

Y

Y

S
|
S

I
|
I

S
|
S

N

N

T

T

A
|
A

E

E

Y

F

G

G

D

D

I

Y

K

V

T

S

G

G

P

P

R

R

I

V

I

I

T

T

E

P

E

D

T

V

K

K

K

E

E

N

M

M

K

K

R

A

V

V

L

L

A

T

D

D

I

I

Q

Q

S

N

G

G

R

N

F

F

V

S

K

N

D

R

F

F

V

I

L

E

D

D

N

N

R

K

A

N

G

G

Q

F

P

K

E

E

L

F

K

Y

A

K

S

L

R

R

I

E

A

E

A

Q

K

H

R

G

H

H

Q

Q

I

I

E

E

Q

K

V

V

G

G

S

R

E

E

L

L

R

R

A

E

M

M

P

P

W

F

I

I

binding magnesium ion: D189 (= D191), E193 (= E195)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G23 (= G23), Y24 (= Y24), G25 (= G25), S26 (= S26), Q27 (= Q27), I46 (≠ L47), R47 (= R48), S51 (≠ T53), L78 (= L80), L79 (≠ A81), P80 (= P82), D81 (= D83), I83 (≠ H85), Q84 (= Q86), V87 (≠ I89), A105 (= A107), H106 (= H108), G130 (= G132), P131 (= P133), G132 (= G134), S248 (= S250), I249 (= I251), S250 (= S252)
binding 3-(methylsulfonyl)-2-oxopropanoic acid: G130 (= G132), P131 (= P133), D189 (= D191), E193 (= E195), E229 (= E231), I233 (= I235), I249 (= I251), S250 (= S252), A253 (= A255)

6c5nA Crystal structure of staphylococcus aureus ketol-acid reductoisomerase with hydroxyoxamate inhibitor 1
59% identity, 96% coverage: 3:328/339 of query aligns to 3:326/326 of 6c5nA

query
sites
6c5nA

V

V

Y

Y

D

D

R

Q

D

D

A

V

D

K

L

T

N

D

L

A

I

L

T

Q

G

G

K

K

I

I

A

A

I

V

V

V

G
|
G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

Q

Q

N

N

L

L

R

K

D

D

S

N

G

G

V

Y

K

-

E

D

V

V

A

V

I

I

A

G

L
x
I

R
|
R

E

P

G

G

S

R

A

-

T
x
S

A

F

K

D

K

K

A

A

E

K

A

E

A

D

G

G

F

F

K

D

V

V

L

F

S

P

N
x
V

K

A

E

E

A

A

A

V

Q

K

W

Q

A

A

D

D

V

V

L

I

M

M

I

V

L
|
L

A
x
L

P
|
P

D
|
D

E

E

H
x
I

Q
|
Q

A

G

A

D

I
x
V

W

Y

E

K

N

N

D

E

L

I

K

E

G

P

N

N

M

L

K

E

P

K

G

H

S

N

A

A

I

L

A

A

F

F

A
|
A

H
|
H

G

G

L

F

N

N

I

I

H

H

F

F

G

G

L

V

I

I

E

Q

A

P

P

P

A

A

D

D

I

V

D

D

V

V

I

F

M

L

I

V

A

A

P

P

K
|
K

G
|
G

P
|
P

G
|
G

H

H

T

L

V

V

R

R

S

R

E

T

Y

F

V

V

R

E

G

G

G

S

G

A

V

V

P

P

C

S

L

L

I

F

A

G

V

I

H

Q

Q

Q

D

D

A

A

S

S

G

G

N

Q

A

A

H

R

D

N

V

I

A

A

L

L

A

S

Y

Y

A

A

S

K

G

G

V

I

G

G

G

A

G

T

R

R

S

A

G

G

I

V

I

I

E

E

T

T

N

T

F

F

R

K

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L
|
L

C
|
C

G

G

A

V

T

S

A

K

L

L

V

I

Q

Q

A

S

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

Q

P

P

E

E

M

L

A

A

Y

Y

F

F

E

E

C

V

L

L

H

H

E
|
E

L

M

K

K

L

L

I
|
I

V

V

D

D

L

L

M

M

Y

Y

E

E

G

G

I

M

A

E

N

N

M

V

R

R

Y

Y

S
|
S

I
|
I

S
|
S

N

N

T

T

A
|
A

E

E

Y

F

G

G

D

D

I

Y

K

V

T

S

G

G

P

P

R

R

I

V

I

I

T

T

E

P

E

D

T

V

K

K

K

E

E

N

M

M

K

K

R

A

V

V

L

L

A

T

D

D

I

I

Q

Q

S

N

G

G

R

N

F

F

V

S

K

N

D

R

F

F

V

I

L

E

D

D

N

N

R

K

A

N

G

G

Q

F

P

K

E

E

L

F

K

Y