SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ga0059261_0167 FitnessBrowser__Korea:Ga0059261_0167 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9R4E4 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Agrobacterium sp. (strain CP4) (see 2 papers)
59% identity, 95% coverage: 17:439/443 of query aligns to 18:443/455 of Q9R4E4

query
sites
Q9R4E4

L

L

R

S

G

G

T

T

L

V

T

R

V

I

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

S

S

L

F

M

M

F

F

A

G

G

G

L

L

A

A

V

S

G

G

E

E

S

T

R

R

I

I

E

T

G

G

L

L

E

E

G

G

E

E

D

D

V

V

L

I

A

N

T

T

A

G

A

K

A

A

M

M

R

Q

A

A

M

M

G

G

A

A

K

R

I

I

E

R

R

K

D

E

G

G

D

D

G

-

V

T

W

W

H

I

V

I

W

D

G

G

V

V

G

G

V

N

G

G

L

L

Q

A

P

P

E

E

T

A

A

P

L

L

E

D

M

F

G

G

N
|
N

S
x
A

G
x
A

T
|
T

S

G

T

C

R

R

L

L

L

T

M

M

G

G

L

L

V

V

A

G

S

V

H

Y

P

D

I

F

T

D

A

S

T

T

F

F

T

I

G

G

D

D

A

A

S

S

L

L

S

T

K

K

R
|
R

P

P

M

M

G

G

R

R

V

V

I

L

D

N

P

P

L

L

S

R

R

E

M

M

G

G

A

V

D

Q

F

V

T

K

S

S

S

E

P

D

G

G

G

D

R

R

L

L

P

P

L

V

T

T

M

L

R

R

G

G

L

P

C

K

P

T

A

P

V

T

P

P

I

I

E

T

Y

Y

T

R

L

V

P

P

V

M

A

A

S

S

A

A

Q

Q

V

V

K

K

S

S

A

A

V

V

L

L

A

A

G

G

L

L

N

N

T

T

P

P

G

G

I

I

T

T

R

T

V

V

I

I

E

E

P

P

V

I

L

M

T

T

R

R

D

D

H

H

S

T

E

E

R

K

M

M

L

L

A

Q

G

G

F

F

G

G

A

A

N

N

L

L

T

T

V

V

E

E

E

T

T

D

P

A

Q

D

G

G

-

V

R

R

I

T

I

I

S

R

I

L

H

E

G

G

E

R

A

G

E

K

F

L

K

T

P

G

Q

Q

Q

V

I

I

T

D

V

V

P

P

G

G

D

D

P

P

S

S

A

T

A

A

F

F

W

P

M

L

V

V

A

A

S

L

I

L

V

V

P

P

G

G

S

S

D

D

I

V

T

T

V

I

A

L

N

N

V

V

G

L

L

M

N

N

P

P

T

T

R

R

I

T

G

G

L

L

I

I

T

L

A

T

L

L

R

Q

M

E

M

M

G

G

A

A

D

D

I

I

T

E

E

V

V

I

N

N

P

P

R

R

I

L

V

A

G

G

E

E

P

D

V

V

A

A

D

D

L

L

R

R

V

V

R

R

H

S

A

S

P

T

L

L

S

K

A

G

I

V

E

T

V

V

P

P

H

E

D

D

L

R

A

A

P

P

S

S

M

M

I

I

D

D

E

E

Y

Y

P

P

V

I

L

L

F

A

V

V

A

A

F

F

A

A

V

E

G

G

R

A

T

T

I

V

A

M

R

N

G

G

A

L

E

E

L

L

R

R

V

V

K
|
K

E

E

S

S

D

D

R
|
R

I

L

A

S

A

A

M

V

V

A

A

N

A

G

L

L

A

K

P

L

N

N

G

G

V

V

P

D

L

C

E

D

E

E

F

G

E

E

D

T

G

S

L

L

A

V

I

V

T

R

G

G

T

R

G

P

G

D

G

G

P

K

-

G

-

L

-

G

I

N

A

A

G

S

G

G

A

A

P

A

V

V

A

A

S

T

K

H

L

L

D

D

H

H

R
|
R

I

I

A

A

M

M

S

S

M

F

A

L

V

V

A

M

G

G

L

L

G

V

A

S

K

E

A

N

D

P

V

V

S

T

I

V

D

D

V

A

S

T

P

M

V

I

A

A

T

T

S

S

Y

F

P

P

G

E

F

F

F

M

S

D

T

L

L

M

D

A

S

G

L

L

KS 28:29 (= KS 27:28) binding
R33 (= R32) binding
NAAT 98:101 (≠ NSGT 98:101) Phosphoenolpyruvate
A100 (≠ G100) mutation to G: Confers resistance to glyphosate.
R128 (= R128) binding
K353 (= K352) binding
R357 (= R356) binding
R405 (= R401) binding

2pqcA Cp4 epsps liganded with (r)-phosphonate tetrahedral reaction intermediate analog (see paper)
59% identity, 95% coverage: 17:439/443 of query aligns to 13:438/445 of 2pqcA

query
sites
2pqcA

L

L

R

S

G

G

T

T

L

V

T

R

V

I

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

S

S

L

F

M

M

F

F

A

G

G

G

L

L

A

A

V

S

G

G

E

E

S

T

R

R

I

I

E

T

G

G

L

L

E

E

G

G

E

E

D
|
D

V

V

L

I

A

N

T

T

A

G

A

K

A

A

M

M

R

Q

A

A

M

M

G

G

A

A

K

R

I

I

E

R

R

K

D

E

G

G

D

D

G

-

V

T

W

W

H

I

V

I

W

D

G

G

V

V

G

G

V

N

G

G

L

L

Q

A

P

P

E

E

T

A

A

P

L

L

E

D

M

F

G

G

N
|
N

S

A

G

A

T
|
T

S

G

T

C

R

R

L

L

L

T

M

M

G

G

L

L

V

V

A

G

S

V

H

Y

P

D

I

F

T

D

A

S

T

T

F

F

T

I

G

G

D

D

A

A

S

S

L

L

S

T

K

K

R
|
R

P

P

M

M

G

G

R

R

V

V

I

L

D

N

P

P

L

L

S

R

R

E

M

M

G

G

A

V

D

Q

F

V

T

K

S

S

S

E

P

D

G

G

G

D

R

R

L

L

P

P

L

V

T

T

M

L

R

R

G

G

L

P

C

K

P

T

A

P

V

T

P

P

I

I

E

T

Y

Y

T

R

L

V

P

P

V

M

A

A

S
|
S

A

A

Q
|
Q

V

V

K

K

S

S

A

A

V

V

L

L

A

A

G

G

L

L

N

N

T

T

P

P

G

G

I

I

T

T

R

T

V

V

I

I

E

E

P

P

V

I

L

M

T

T

R

R

D

D

H

H

S

T

E

E

R

K

M

M

L

L

A

Q

G

G

F

F

G

G

A

A

N

N

L

L

T

T

V

V

E

E

E

T

T

D

P

A

Q

D

G

G

-

V

R

R

I

T

I

I

S

R

I

L

H

E

G

G

E

R

A

G

E

K

F

L

K

T

P

G

Q

Q

Q

V

I

I

T

D

V

V

P

P

G

G

D

D

P

P

S

S

A

T

A

A

F

F

W

P

M

L

V

V

A

A

S

L

I

L

V

V

P

P

G

G

S

S

D

D

I

V

T

T

V

I

A

L

N

N

V

V

G

L

L

M

N

N

P

P

T

T

R

R

I

T

G

G

L

L

I

I

T

L

A

T

L

L

R

Q

M

E

M

M

G

G

A

A

D

D

I

I

T

E

E

V

V

I

N

N

P

P

R

R

I

L

V

A

G

G

E

E

P

D

V

V

A

A

D

D

L

L

R

R

V

V

R

R

H

S

A

S

P

T

L

L

S

K

A

G

I

V

E

T

V

V

P

P

H

E

D

D

L

R

A

A

P

P

S

S

M

M

I

I

D
|
D

E

E

Y

Y

P

P

V

I

L

L

F

A

V

V

A

A

F

F

A

A

V

E

G

G

R

A

T

T

I

V

A

M

R

N

G

G

A

L

E

E

L

L

R

R

V

V

K
|
K

E
|
E

S

S

D

D

R
|
R

I

L

A

S

A

A

M

V

V

A

A

N

A

G

L

L

A

K

P

L

N

N

G

G

V

V

P

D

L

C

E

D

E

E

F

G

E

E

D

T

G

S

L

L

A

V

I

V

T

R

G

G

T

R

G

P

G

D

G

G

P

K

-

G

-

L

-

G

I

N

A

A

G

S

G

G

A

A

P

A

V

V

A

A

S

T

K

H

L

L

D

D

H
|
H

R
|
R

I

I

A

A

M

M

S

S

M

F

A

L

V

V

A

M

G

G

L

L

G

V

A

S

K

E

A

N

D

P

V

V

S

T

I

V

D

D

V

A

S

T

P

M

V

I

A

A

T
|
T

S

S

Y

F

P

P

G

E

F

F

F

M

S

D

T

L

L

M

D

A

S

G

L

L

active site: K23 (= K27), S24 (= S28), D50 (= D54), N93 (= N98), R123 (= R128), D321 (= D325), E349 (= E353), H399 (= H400), R400 (= R401), T426 (= T427)
binding [3r-[3a,4a,5b(r*)]]-5-(1-carboxy-1-phosphonoethoxy)-4-hydroxy-3-(phosphonooxy)-1-cyclohexene-1-carboxylic acid: K23 (= K27), S24 (= S28), R28 (= R32), T96 (= T101), R123 (= R128), S168 (= S173), Q170 (= Q175), D321 (= D325), K348 (= K352), E349 (= E353), R352 (= R356), R400 (= R401)

2pqbA Cp4 epsps liganded with (r)-difluoromethyl tetrahedral intermediate analog (see paper)
59% identity, 95% coverage: 17:439/443 of query aligns to 13:438/445 of 2pqbA

query
sites
2pqbA

L

L

R

S

G

G

T

T

L

V

T

R

V

I

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

S

S

L

F

M

M

F

F

A

G

G

G

L

L

A

A

V

S

G

G

E

E

S

T

R

R

I

I

E

T

G

G

L

L

E

E

G

G

E

E

D
|
D

V

V

L

I

A

N

T

T

A

G

A

K

A

A

M

M

R

Q

A

A

M

M

G

G

A

A

K

R

I

I

E

R

R

K

D

E

G

G

D

D

G

-

V

T

W

W

H

I

V

I

W

D

G

G

V

V

G

G

V

N

G

G

L

L

Q

A

P

P

E

E

T

A

A

P

L

L

E

D

M

F

G

G

N
|
N

S

A

G
x
A

T
|
T

S

G

T

C

R

R

L

L

L

T

M

M

G

G

L

L

V

V

A

G

S

V

H

Y

P

D

I

F

T

D

A

S

T

T

F

F

T

I

G

G

D

D

A

A

S

S

L

L

S

T

K

K

R
|
R

P

P

M

M

G

G

R

R

V

V

I

L

D

N

P

P

L

L

S

R

R

E

M

M

G

G

A

V

D

Q

F

V

T

K

S

S

S

E

P

D

G

G

G

D

R

R

L

L

P

P

L

V

T

T

M

L

R

R

G

G

L

P

C

K

P

T

A

P

V

T

P

P

I

I

E

T

Y

Y

T

R

L

V

P

P

V

M

A

A

S
|
S

A

A

Q
|
Q

V

V

K

K

S

S

A

A

V

V

L

L

A

A

G

G

L

L

N

N

T

T

P

P

G

G

I

I

T

T

R

T

V

V

I

I

E

E

P

P

V

I

L

M

T

T

R

R

D

D

H

H

S

T

E

E

R

K

M

M

L

L

A

Q

G

G

F

F

G

G

A

A

N

N

L

L

T

T

V

V

E

E

E

T

T

D

P

A

Q

D

G

G

-

V

R

R

I

T

I

I

S

R

I

L

H

E

G

G

E

R

A

G

E

K

F

L

K

T

P

G

Q

Q

Q

V

I

I

T

D

V

V

P

P

G

G

D

D

P

P

S

S

A

T

A

A

F

F

W

P

M

L

V

V

A

A

S

L

I

L

V

V

P

P

G

G

S

S

D

D

I

V

T

T

V

I

A

L

N

N

V

V

G

L

L

M

N

N

P

P

T

T

R

R

I

T

G

G

L

L

I

I

T

L

A

T

L

L

R

Q

M

E

M

M

G

G

A

A

D

D

I

I

T

E

E

V

V

I

N

N

P

P

R

R

I

L

V

A

G

G

E

E

P

D

V

V

A

A

D

D

L

L

R

R

V

V

R

R

H

S

A

S

P

T

L

L

S

K

A

G

I

V

E

T

V

V

P

P

H

E

D

D

L

R

A

A

P

P

S

S

M

M

I

I

D
|
D

E

E

Y

Y

P

P

V

I

L

L

F

A

V

V

A

A

F

F

A

A

V

E

G

G

R

A

T

T

I

V

A

M

R

N

G

G

A

L

E

E

L

L

R

R

V

V

K
|
K

E
|
E

S

S

D

D

R
|
R

I

L

A

S

A

A

M

V

V

A

A

N

A

G

L

L

A

K

P

L

N

N

G

G

V

V

P

D

L

C

E

D

E

E

F

G

E

E

D

T

G

S

L

L

A

V

I

V

T

R

G

G

T

R

G

P

G

D

G

G

P

K

-

G

-

L

-

G

I

N

A

A

G

S

G

G

A

A

P

A

V

V

A

A

S

T

K

H

L

L

D

D

H
|
H

R
|
R

I

I

A

A

M

M

S

S

M

F

A

L

V

V

A

M

G

G

L

L

G

V

A

S

K

E

A

N

D

P

V

V

S

T

I

V

D

D

V

A

S

T

P

M

V

I

A

A

T
|
T

S

S

Y

F

P

P

G

E

F

F

F

M

S

D

T

L

L

M

D

A

S

G

L

L

active site: K23 (= K27), S24 (= S28), D50 (= D54), N93 (= N98), R123 (= R128), D321 (= D325), E349 (= E353), H399 (= H400), R400 (= R401), T426 (= T427)
binding (3r,4s,5r)-5-[(1r)-1-carboxy-2,2-difluoro-1-(phosphonooxy)ethoxy]-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid: K23 (= K27), S24 (= S28), R28 (= R32), A95 (≠ G100), T96 (= T101), R123 (= R128), S168 (= S173), Q170 (= Q175), D321 (= D325), K348 (= K352), E349 (= E353), R352 (= R356), R400 (= R401)

2ggaA Cp4 epsp synthase liganded with s3p and glyphosate (see paper)
59% identity, 95% coverage: 17:439/443 of query aligns to 13:438/445 of 2ggaA

query
sites
2ggaA

L

L

R

S

G

G

T

T

L

V

T

R

V

I

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

S

S

L

F

M

M

F

F

A

G

G

G

L

L

A

A

V

S

G

G

E

E

S

T

R

R

I

I

E

T

G

G

L

L

E

E

G

G

E

E

D
|
D

V

V

L

I

A

N

T

T

A

G

A

K

A

A

M

M

R

Q

A

A

M

M

G

G

A

A

K

R

I

I

E

R

R

K

D

E

G

G

D

D

G

-

V

T

W

W

H

I

V

I

W

D

G

G

V

V

G

G

V

N

G

G

L

L

Q

A

P

P

E

E

T

A

A

P

L

L

E

D

M

F

G

G

N
|
N

S
x
A

G
x
A

T
|
T

S

G

T

C

R

R

L

L

L

T

M

M

G

G

L

L

V

V

A

G

S

V

H

Y

P

D

I

F

T

D

A

S

T

T

F

F

T

I

G

G

D

D

A

A

S

S

L

L

S

T

K

K

R
|
R

P

P

M

M

G

G

R

R

V

V

I

L

D

N

P

P

L

L

S

R

R

E

M

M

G

G

A

V

D

Q

F

V

T

K

S

S

S

E

P

D

G

G

G

D

R

R

L

L

P

P

L

V

T

T

M

L

R

R

G

G

L

P

C

K

P

T

A

P

V

T

P

P

I

I

E

T

Y

Y

T

R

L

V

P

P

V

M

A

A

S
|
S

A
|
A

Q
|
Q

V

V

K

K

S

S

A

A

V

V

L

L

A

A

G

G

L

L

N

N

T

T

P

P

G

G

I

I

T

T

R

T

V

V

I

I

E

E

P

P

V

I

L

M

T

T

R
|
R

D

D

H

H

S

T

E

E

R

K

M

M

L

L

A

Q

G

G

F

F

G

G

A

A

N

N

L

L

T

T

V

V

E

E

E

T

T

D

P

A

Q

D

G

G

-

V

R

R

I

T

I

I

S

R

I

L

H

E

G

G

E

R

A

G

E

K

F

L

K

T

P

G

Q

Q

Q

V

I

I

T

D

V

V

P

P

G

G

D

D

P

P

S

S

A

T

A

A

F

F

W

P

M

L

V

V

A

A

S

L

I

L

V

V

P

P

G

G

S

S

D

D

I

V

T

T

V

I

A

L

N

N

V

V

G

L

L

M

N

N

P

P

T

T

R

R

I

T

G

G

L

L

I

I

T

L

A

T

L

L

R

Q

M

E

M

M

G

G

A

A

D

D

I

I

T

E

E

V

V

I

N

N

P

P

R

R

I

L

V

A

G

G

E

E

P

D

V

V

A

A

D

D

L

L

R

R

V

V

R

R

H

S

A

S

P

T

L

L

S

K

A

G

I

V

E

T

V

V

P

P

H

E

D

D

L

R

A

A

P

P

S

S

M

M

I

I

D
|
D

E

E

Y

Y

P

P

V

I

L

L

F

A

V

V

A

A

F

F

A

A

V

E

G

G

R

A

T

T

I

V

A

M

R

N

G

G

A

L

E

E

L

L

R

R

V

V

K
|
K

E
|
E

S

S

D

D

R
|
R

I

L

A

S

A

A

M

V

V

A

A

N

A

G

L

L

A

K

P

L

N

N

G

G

V

V

P

D

L

C

E

D

E

E

F

G

E

E

D

T

G

S

L

L

A

V

I

V

T

R

G

G

T

R

G

P

G

D

G

G

P

K

-

G

-

L

-

G

I

N

A

A

G

S

G

G

A

A

P

A

V

V

A

A

S

T

K

H

L

L

D

D

H
|
H

R
|
R

I

I

A

A

M

M

S

S

M

F

A

L

V

V

A

M

G

G

L

L

G

V

A

S

K

E

A

N

D

P

V

V

S

T

I

V

D

D

V

A

S

T

P

M

V

I

A

A

T
|
T

S

S

Y

F

P

P

G

E

F

F

F

M

S

D

T

L

L

M

D

A

S

G

L

L

active site: K23 (= K27), S24 (= S28), D50 (= D54), N93 (= N98), R123 (= R128), D321 (= D325), E349 (= E353), H399 (= H400), R400 (= R401), T426 (= T427)
binding glyphosate: K23 (= K27), A94 (≠ S99), A95 (≠ G100), T96 (= T101), R123 (= R128), D321 (= D325), E349 (= E353), R352 (= R356), R400 (= R401)
binding shikimate-3-phosphate: S24 (= S28), R28 (= R32), S168 (= S173), A169 (= A174), Q170 (= Q175), R195 (= R200), D321 (= D325), K348 (= K352)

2gg6A Cp4 epsp synthase liganded with s3p (see paper)
59% identity, 95% coverage: 17:439/443 of query aligns to 13:438/445 of 2gg6A

query
sites
2gg6A

L

L

R

S

G

G

T

T

L

V

T

R

V

I

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

S

S

L

F

M

M

F

F

A

G

G

G

L

L

A

A

V

S

G

G

E

E

S

T

R

R

I

I

E

T

G

G

L

L

E

E

G

G

E

E

D
|
D

V

V

L

I

A

N

T

T

A

G

A

K

A

A

M

M

R

Q

A

A

M

M

G

G

A

A

K

R

I

I

E

R

R

K

D

E

G

G

D

D

G

-

V

T

W

W

H

I

V

I

W

D

G

G

V

V

G

G

V

N

G

G

L

L

Q

A

P

P

E

E

T

A

A

P

L

L

E

D

M

F

G

G

N
|
N

S

A

G

A

T
|
T

S

G

T

C

R

R

L

L

L

T

M

M

G

G

L

L

V

V

A

G

S

V

H

Y

P

D

I

F

T

D

A

S

T

T

F

F

T

I

G

G

D

D

A

A

S

S

L

L

S

T

K

K

R
|
R

P

P

M

M

G

G

R

R

V

V

I

L

D

N

P

P

L

L

S

R

R

E

M

M

G

G

A

V

D

Q

F

V

T

K

S

S

S

E

P

D

G

G

G

D

R

R

L

L

P

P

L

V

T

T

M

L

R

R

G

G

L

P

C

K

P

T

A

P

V

T

P

P

I

I

E

T

Y

Y

T

R

L

V

P

P

V

M

A

A

S
|
S

A

A

Q
|
Q

V

V

K

K

S

S

A

A

V

V

L

L

A

A

G

G

L

L

N

N

T

T

P

P

G

G

I

I

T

T

R

T

V

V

I

I

E

E

P

P

V

I

L

M

T

T

R

R

D

D

H

H

S

T

E

E

R

K

M

M

L

L

A

Q

G

G

F

F

G

G

A

A

N

N

L

L

T

T

V

V

E

E

E

T

T

D

P

A

Q

D

G

G

-

V

R

R

I

T

I

I

S

R

I

L

H

E

G

G

E

R

A

G

E

K

F

L

K

T

P

G

Q

Q

Q

V

I

I

T

D

V

V

P

P

G

G

D

D

P

P

S

S

A

T

A

A

F

F

W

P

M

L

V

V

A

A

S

L

I

L

V

V

P

P

G

G

S

S

D

D

I

V

T

T

V

I

A

L

N

N

V

V

G

L

L

M

N

N

P

P

T

T

R

R

I

T

G

G

L

L

I

I

T

L

A

T

L

L

R

Q

M

E

M

M

G

G

A

A

D

D

I

I

T

E

E

V

V

I

N

N

P

P

R

R

I

L

V

A

G

G

E

E

P

D

V

V

A

A

D

D

L

L

R

R

V

V

R

R

H

S

A

S

P

T

L

L

S

K

A

G

I

V

E

T

V

V

P

P

H

E

D

D

L

R

A

A

P

P

S

S

M

M

I

I

D
|
D

E

E

Y

Y

P

P

V

I

L

L

F

A

V

V

A

A

F

F

A

A

V

E

G

G

R

A

T

T

I

V

A

M

R

N

G

G

A

L

E

E

L

L

R

R

V

V

K
|
K

E
|
E

S

S

D

D

R

R

I

L

A

S

A

A

M

V

V

A

A

N

A

G

L

L

A

K

P

L

N

N

G

G

V

V

P

D

L

C

E

D

E

E

F

G

E

E

D

T

G

S

L

L

A

V

I

V

T

R

G

G

T

R

G

P

G

D

G

G

P

K

-

G

-

L

-

G

I

N

A

A

G

S

G

G

A

A

P

A

V

V

A

A

S

T

K

H

L

L

D

D

H
|
H

R
|
R

I

I

A

A

M

M

S

S

M

F

A

L

V

V

A

M

G

G

L

L

G

V

A

S

K

E

A

N

D

P

V

V

S

T

I

V

D

D

V

A

S

T

P

M

V

I

A

A

T
|
T

S

S

Y

F

P

P

G

E

F

F

F

M

S

D

T

L

L

M

D

A

S

G

L

L

active site: K23 (= K27), S24 (= S28), D50 (= D54), N93 (= N98), R123 (= R128), D321 (= D325), E349 (= E353), H399 (= H400), R400 (= R401), T426 (= T427)
binding shikimate-3-phosphate: S24 (= S28), R28 (= R32), T96 (= T101), S168 (= S173), Q170 (= Q175), D321 (= D325), K348 (= K352)

Q83E11 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
49% identity, 94% coverage: 17:432/443 of query aligns to 11:418/438 of Q83E11

query
sites
Q83E11

L

L

R

S

G

G

T

E

L

I

T

C

V

V

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

S

A

L

V

M

L

F

L

A

A

G

A

L

I

A

A

V

E

G

G

E

Q

S

T

R

Q

I

V

E

D

G

G

L

F

L

L

E

M

G

G

E

A

D

D

V

N

L

L

A

A

T

M

A

V

A

S

A

A

M

L

R

Q

A

Q

M

M

G

G

A

A

K

S

I

I

E

Q

R

V

-

I

D

E

G

D

D

E

G

N

V

I

W

L

H

V

V

V

W

E

G

G

V

V

G

G

V

M

G

T

G

G

L

L

L

Q

Q

A

P

P

E

P

T

E

A

A

L

L

E

D

M

C

G

G

N
|
N

S
|
S

G
|
G

T
|
T

S

A

T

I

R

R

L

L

M

S

G

G

L

L

V

L

A

A

S

G

H

Q

P

P

I

F

T

N

A

T

T

V

F

L

T

T

G

G

D

D

A

S

S

S

L

L

S

Q

K

R

R
|
R

P

P

M

M

G

K

R

R

V

I

I

I

D

D

P

P

L

L

S

T

R

L

M

M

G

G

A

A

D

K

F

I

T

D

S

S

S

T

P

-

G

G

G

N

R

V

L

P

P

P

L

L

T

K

M

I

R

Y

G

G

L

N

C

P

P

R

A

L

V

T

P

G

I

I

E

H

Y

Y

T

Q

L

L

P

P

V

M

A

A

S

S

A

A

Q

Q

V

V

K

K

S

S

A

C

V

L

L

L

A

A

G

G

L

L

N

Y

T

A

P

R

G

G

I

K

T

T

R

C

V

I

I

T

E

E

P

P

V

A

L

P

T

S

R

R

D

D

H

H

S

T

E

E

R

R

M

L

L

L

A

K

G

H

F

F

G

-

A

-

N

H

L

Y

T

T

V

L

E

Q

E

K

T

D

P

K

Q

Q

G

S

R

-

I

-

I

I

S

C

I

V

H

S

G

G

E

G

A

G

E

K

F

L

K

K

P

A

Q

N

Q

D

I

I

T

S

V

I

P

P

G

G

D

D

P

I

S

S

A

A

F

F

W

F

M

I

V

V

A

A

S

T

I

I

V

T

P

P

G

G

S

S

D

A

I

I

T

R

V

L

A

C

N

R

V

V

G

G

L

V

N

N

P

P

T

T

R

R

I

L

G

G

L

V

I

I

T

N

A

L

L

L

R

K

M

M

G

G

A

A

D

D

I

I

T

E

E

V

V

T

N

H

P

Y

R

T

I

E

V

K

G

N

G

E

E

E

P

P

V

T

A

A

D

D

L

I

R

T

V

V

R

R

H

H

A

A

P

R

L

L

S

K

A

G

I

I

E

D

V

I

P

P

H

P

D

D

L

Q

A

V

P

P

S

L

M

T

I

I

D
|
D

E

E

Y

F

P

P

V

V

L

L

F

L

V

I

A

A

F

V

A

A

V

Q

G

G

R

K

T

T

I

V

A

L

R

R

G

D

A

A

E

A

E

E

L

L

R

R

V

V

K
|
K

E

E

S

T

D

D

R
|
R

I

I

A

A

M

M

V

V

A

D

A

G

L

L

A

Q

P

K

N

L

G

G

V

I

P

A

L

A

E

E

E

S

F

L

E

P

D

D

G

G

L

V

A

I

I

I

T

Q

G

-

T

-

G

-

G

G

P

T

I

L

A

E

G

G

A

E

P

-

V

V

A

N

S

S

K

Y

L

D

D

D

H

H

R
|
R

I

I

A

A

M

M

S

A

M

F

A

A

V

V

A

A

G

G

L

T

G

L

A

A

K

K

A

G

D

P

V

V

S

R

I

I

D

R

D

N

V

C

S

D

P

N

V

V

A

K

T

T

S

S

Y

F

P

P

G

N

F

F

KS 21:22 (= KS 27:28) binding
R26 (= R32) binding
NSGT 93:96 (= NSGT 98:101) Phosphoenolpyruvate
R123 (= R128) binding
D315 (= D325) active site, Proton acceptor
K342 (= K352) binding
R346 (= R356) binding
R387 (= R401) binding

3slhD 1.70 angstrom resolution structure of 3-phosphoshikimate 1- carboxyvinyltransferase (aroa) from coxiella burnetii in complex with shikimate-3-phosphate and glyphosate
49% identity, 94% coverage: 17:432/443 of query aligns to 13:420/440 of 3slhD

query
sites
3slhD

L

L

R

S

G

G

T

E

L

I

T

C

V

V

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

S

A

L

V

M

L

F

L

A

A

G

A

L

I

A

A

V

E

G

G

E

Q

S

T

R

Q

I

V

E

D

G

G

L

F

L

L

E

M

G

G

E

A

D
|
D

V

N

L

L

A

A

T

M

A

V

A

S

A

A

M

L

R

Q

A

Q

M

M

G

G

A

A

K

S

I

I

E

Q

R

V

-

I

D

E

G

D

D

E

G

N

V

I

W

L

H

V

V

V

W

E

G

G

V

V

G

G

V

M

G

T

G

G

L

L

L

Q

Q

A

P

P

E

P

T

E

A

A

L

L

E

D

M

C

G

G

N
|
N

S

S

G
|
G

T
|
T

S

A

T

I

R

R

L

L

M

S

G

G

L

L

V

L

A

A

S

G

H

Q

P

P

I

F

T

N

A

T

T

V

F

L

T

T

G

G

D

D

A

S

S

S

L

L

S

Q

K

R

R
|
R

P

P

M

M

G

K

R

R

V

I

I

I

D

D

P

P

L

L

S

T

R

L

M

M

G

G

A

A

D

K

F

I

T

D

S

S

S

T

P

-

G

G

G

N

R

V

L

P

P

P

L

L

T

K

M

I

R

Y

G

G

L

N

C

P

P

R

A

L

V

T

P

G

I

I

E

H

Y

Y

T

Q

L

L

P

P

V

M

A

A

S
|
S

A

A

Q
|
Q

V

V

K

K

S

S

A

C

V

L

L

L

A

A

G

G

L

L

N

Y

T

A

P

R

G

G

I

K

T

T

R

C

V

I

I

T

E

E

P

P

V

A

L

P

T

S

R
|
R

D

D

H

H

S

T

E

E

R

R

M

L

L

L

A

K

G

H

F

F

G

-

A

-

N

H

L

Y

T

T

V

L

E

Q

E

K

T

D

P

K

Q

Q

G

S

R

-

I

-

I

I

S

C

I

V

H

S

G

G

E

G

A

G

E

K

F

L

K

K

P

A

Q

N

Q

D

I

I

T

S

V

I

P

P

G

G

D

D

P

I

S

S

A

A

F

F

W

F

M

I

V

V

A

A

S

T

I

I

V

T

P

P

G

G

S

S

D

A

I

I

T

R

V

L

A

C

N

R

V

V

G

G

L

V

N

N

P

P

T

T

R

R

I

L

G

G

L

V

I

I

T

N

A

L

L

L

R

K

M

M

G

G

A

A

D

D

I

I

T

E

E

V

V

T

N

H

P

Y

R

T

I

E

V

K

G

N

G

E

E

E

P

P

V

T

A

A

D

D

L

I

R

T

V

V

R

R

H

H

A

A

P

R

L

L

S

K

A

G

I

I

E

D

V

I

P

P

H

P

D

D

L

Q

A

V

P

P

S

L

M

T

I

I

D
|
D

E

E

Y

F

P

P

V

V

L

L

F

L

V

I

A

A

F

V

A

A

V

Q

G

G

R

K

T

T

I

V

A

L

R

R

G

D

A

A

E

A

E

E

L

L

R

R

V

V

K
|
K

E
|
E

S

T

D

D

R
|
R

I

I

A

A

M

M

V

V

A

D

A

G

L

L

A

Q

P

K

N

L

G

G

V

I

P

A

L

A

E

E

E

S

F

L

E

P

D

D

G

G

L

V

A

I

I

I

T

Q

G

-

T

-

G

-

G

G

P

T

I

L

A

E

G

G

A

E

P

-

V

V

A

N

S

S

K

Y

L

D

D

D

H
|
H

R
|
R

I

I

A

A

M

M

S

A

M

F

A

A

V

V

A

A

G

G

L

T

G

L

A

A

K

K

A

G

D

P

V

V

S

R

I

I

D

R

D

N

V

C

S

D

P

N

V

V

A

K

T
|
T

S

S

Y

F

P

P

G

N

F

F

active site: K23 (= K27), S24 (= S28), D50 (= D54), N95 (= N98), R125 (= R128), D317 (= D325), E345 (= E353), H388 (= H400), R389 (= R401), T415 (= T427)
binding glyphosate: K23 (= K27), G97 (= G100), T98 (= T101), R125 (= R128), Q171 (= Q175), D317 (= D325), E345 (= E353), R348 (= R356), H388 (= H400), R389 (= R401)
binding shikimate-3-phosphate: S24 (= S28), R28 (= R32), S169 (= S173), Q171 (= Q175), R196 (= R200), D317 (= D325), K344 (= K352)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S24 (= S28), R28 (= R32), T98 (= T101), Q171 (= Q175), R196 (= R200), D317 (= D325), K344 (= K352)

4egrA 2.50 angstrom resolution structure of 3-phosphoshikimate 1- carboxyvinyltransferase (aroa) from coxiella burnetii in complex with phosphoenolpyruvate
49% identity, 94% coverage: 17:432/443 of query aligns to 13:416/434 of 4egrA

query
sites
4egrA

L

L

R

S

G

G

T

E

L

I

T

C

V

V

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R

R

S

A

L

V

M

L

F

L

A

A

G

A

L

I

A

A

V

E

G

G

E

Q

S

T

R

Q

I

V

E

D

G

G

L

F

L

L

E

M

G

G

E

A

D
|
D

V

N

L

L

A

A

T

M

A

V

A

S

A

A

M

L

R

Q

A

Q

M

M

G

G

A

A

K

S

I

I

E

Q

R

V

-

I

D

E

G

D

D

E

G

N

V

I

W

L

H

V

V

V

W

E

G

G

V

V

G

G

V

M

G

T

G

G

L

L

L

Q

Q

A

P

P

E

P

T

E

A

A

L

L

E

D

M

C

G

G

N
|
N

S

S

G
|
G

T
|
T

S

A

T

I

R

R

L

L

M

S

G

G

L

L

V

L

A

A

S

G

H

Q

P

P

I

F

T

N

A

T

T

V

F

L

T

T

G

G

D

D

A

S

S

S

L

L

S

Q

K

R

R
|
R

P

P

M

M

G

K

R

R

V

I

I

I

D

D

P

P

L

L

S

T

R

L

M

M

G

G

A

A

D

K

F

I

T

D

S

S

S

T

P

-

G

G

G

N

R

V

L

P

P

P

L

L

T

K

M

I

R

Y

G

G

L

N

C

P

P

R

A

L

V

T

P

G

I

I

E

H

Y

Y

T

Q

L

L

P

P

V

M

A

A

S

S

A

A

Q

Q

V

V

K

K

S

S

A

C

V

L

L

L

A

A

G

G

L

L

N

Y

T

A

P

R

G

G

I

K

T

T

R

C

V

I

I

T

E

E

P

P

V

A

L

P

T

S

R

R

D

D

H

H

S

T

E

E

R

R

M

L

L

L

A

K

G

H

F

F

G

H

A

Y

N

T

L

L

T

-

V

-

E

-

T

-

P

-

Q

Q

G

K

R

Q

I

S

I

I

S

C

I

V

H

S

G

G

E

G

A

G

E

K

F

L

K

K

P

A

Q

N

Q

D

I

I

T

S

V

I

P

P

G

G

D

D

P

I

S

S

A

A

F

F

W

F

M

I

V

V

A

A

S

T

I

I

V

T

P

P

G

G

S

S

D

A

I

I

T

R

V

L

A

C

N

R

V

V

G

G

L

V

N

N

P

P

T

T

R

R

I

L

G

G

L

V

I

I

T

N

A

L

L

L

R

K

M

M

G

G

A

A

D

D

I

I

T

-

E

E

V

V

N

T

P

-

R

H

I

Y

V

T

G

E

G

K

E

E

P

P

V

T

A

A

D

D

L

I

R

T

V

V

R

R

H

H

A

A

P

R

L

L

S

K

A

G

I

I

E

D

V

I

P

P

H

P

D

D

L

Q

A

V

P

P

S

L

M

T

I

I

D
|
D

E

E

Y

F

P

P

V

V

L

L

F

L

V

I

A

A

F

V

A

A

V

Q

G

G

R

K

T

T

I

V

A

L

R

R

G

D

A

A

E

A

E

E

L

L

R

R

V

V

K

K

E
|
E

S

T

D

D

R
|
R

I

I

A

A

M

M

V

V

A

D

A

G

L

L

A

Q

P

K

N

L

G

G

V

I

P

A

L

A

E

E

E

S

F

L

E

P

D

D

G

G

L

V

A

I

I

I

T

Q

G

-

T

-

G

-

G

G

P

T

I

L

A

E

G

G

A

E

P

-

V

V

A

N

S

S

K

Y

L

D

D

D

H
|
H

R
|
R

I

I

A

A

M

M

S

A

M

F

A

A

V

V

A

A

G

G

L

T

G

L

A

A

K

K

A

G

D

P

V

V

S

R

I

I

D

R

D

N

V

C

S

D

P

N

V

V

A

K

T
|
T

S

S

Y

F

P

P

G

N

F

F

active site: K23 (= K27), S24 (= S28), D50 (= D54), N95 (= N98), R125 (= R128), D313 (= D325), E341 (= E353), H384 (= H400), R385 (= R401), T411 (= T427)
binding phosphoenolpyruvate: K23 (= K27), G97 (= G100), T98 (= T101), R125 (= R128), D313 (= D325), E341 (= E353), R344 (= R356), R385 (= R401)

1rf6A Structural studies of streptococcus pneumoniae epsp synthase in s3p- glp bound state (see paper)
44% identity, 95% coverage: 17:439/443 of query aligns to 10:424/427 of 1rf6A

query
sites
1rf6A

L

L

R

H

G

G

T

I

L

I

T

R

V

V

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

S

S

L

I

M

I

F

F

A

G

G

S

L

L

A

A

V

E

G

G

E

E

S

T

R

K

I

V

E

Y

G

D

L

I

L

L

E

R

G

G

E

E

D
|
D

V

V

L

L

A

S

T

T

A

M

A

Q

A

V

M

F

R

R

A

D

M

L

G

G

A

V

K

E

I

I

E

E

R

-

D

D

G

K

D

D

G

G

V

V

W

I

H

T

V

V

W

Q

G

G

V

V

G

G

V

M

G

A

G

G

L

L

L

K

Q

A

P

P

E

Q

T

N

A

A

L

L

E

N

M

M

G

G

N
|
N

S

S

G
|
G

T
|
T

S

S

T

I

R

R

L

L

L

I

M

S

G

G

L

V

V

L

A

A

S

G

H

A

P

D

I

F

T

E

A

V

T

E

F

M

T

F

G

G

D

D

A
x
D

S

S

L

L

S

S

K

K

R
|
R

P

P

M

M

G

D

R

R

V

V

I

T

D

L

P

P

L

L

S

K

R

K

M

M

G

G

A

V

D

S

F

I

T

S

S

G

S

Q

P

T

G

E

G

R

R

D

L

L

-

P

P

P

L

L

T

R

M

L

R

K

G

G

L

T

C

K

P

N

A

L

V

R

P

P

I

I

E

H

Y

Y

T

E

L

L

P

P

V

I

A

A

S
|
S

A

A

Q
|
Q

V

V

K

K

S

S

A

A

V

L

L

M

L

F

A

A

G

A

L

L

N

Q

T

A

P

K

G

G

I

E

T

S

R

V

V

I

I

I

E

E

P

K

V

E

L

Y

T

T

R
|
R

D

N

H

H

S

T

E

E

R

D

M

M

L

L

A

Q

G

Q

F

F

G

G

A

G

N

H

L

L

T

S

V

V

E

D

E

-

T

-

P

-

Q

-

G

G

R

K

I

K

I

I

S

T

I

V

H

Q

G

G

E

P

A

Q

E

K

F

L

K

T

P

G

Q

Q

Q

K

I

V

T

V

V

V

P

P

G

G

D

D

P

I

S

S

A

A

F

F

W

W

M

L

V

V

A

A

A

G

S

L

I

I

V

A

P

P

G

N

S

S

D

R

I

L

T

V

V

L

A

Q

N

N

V

V

G

G

L

I

N

N

P

E

T

T

R

R

I

T

G

G

L

I

I

I

T

D

A

V

L

I

R

R

M

A

M

M

G

G

A

G

-

K

-

L

D

E

I

I

T

T

E

E

V

I

N

D

P

P

R

V

I

-

V

-

G

-

E

A

P

K

V

S

A

A

D

T

L

L

R

I

V

V

R

E

H

S

A

S

P

D

L

L

S

K

A

G

I

T

E

E

V

I

P

C

H

G

D

A

L

L

A

I

P

P

S

R

M

L

I
|
I

D
|
D

E

E

Y

L

P

P

V

I

L

I

F

A

V

L

A

L

A

A

A

T

F

Q

A

A

V

Q

G

G

R

V

T

T

I

V

A

I

R

K

G

D

A

A

E

E

L

L

R

K

V

V

K
|
K

E
|
E

S

T

D

D

R
|
R

I

I

A

Q

A

V

M

V

V

A

A

D

A

A

L

L

A

N

P

S

N

M

G

G

V

A

P

D

L

I

E

T

E

P

F

T

E

A

D

D

G

G

L

M

A

I

I

I

T

K

G

G

T

K

G

S

G

A

G

-

P

-

I

-

A

L

G

H

G

G

A

A

P

R

V

V

A

N

S

T

K

F

L

G

D

D

H
|
H

R
|
R

I

I

A

G

M

M

S

M

M

T

A

A

V

I

A

A

G

A

-

L

L

L

G

V

A

A

K

D

A

G

D

E

V

V

S

E

I

L

D

D

D

R

V

A

S

E

P

A

V

I

A

N

T
|
T

S

S

Y

Y

P

P

G

S

F

F

S

D

T

D

L

L

D

E

S

S

L

L

active site: K20 (= K27), S21 (= S28), D47 (= D54), N90 (= N98), D115 (≠ A123), R120 (= R128), D312 (= D325), E340 (= E353), H384 (= H400), R385 (= R401), T412 (= T427)
binding glyphosate: K20 (= K27), G92 (= G100), T93 (= T101), R120 (= R128), Q168 (= Q175), D312 (= D325), E340 (= E353), R343 (= R356), H384 (= H400), R385 (= R401)
binding shikimate-3-phosphate: S21 (= S28), R25 (= R32), S166 (= S173), Q168 (= Q175), R193 (= R200), I311 (= I324), D312 (= D325), K339 (= K352)

1rf4A Structural studies of streptococcus pneumoniae epsp synthase, tetrahedral intermediate bound state (see paper)
44% identity, 95% coverage: 17:439/443 of query aligns to 10:424/427 of 1rf4A

query
sites
1rf4A

L

L

R

H

G

G

T

I

L

I

T

R

V

V

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

S

S

L

I

M

I

F

F

A

G

G

S

L

L

A

A

V

E

G

G

E

E

S

T

R

K

I

V

E

Y

G

D

L

I

L

L

E

R

G

G

E

E

D
|
D

V

V

L

L

A

S

T

T

A

M

A

Q

A

V

M

F

R

R

A

D

M

L

G

G

A

V

K

E

I

I

E

E

R

-

D

D

G

K

D

D

G

G

V

V

W

I

H

T

V

V

W

Q

G

G

V

V

G

G

V

M

G

A

G

G

L

L

L

K

Q

A

P

P

E

Q

T

N

A

A

L

L

E

N

M

M

G

G

N
|
N

S

S

G
|
G

T
|
T

S

S

T

I

R

R

L

L

L

I

M

S

G

G

L

V

V

L

A

A

S

G

H

A

P

D

I

F

T

E

A

V

T

E

F

M

T

F

G

G

D

D

A
x
D

S

S

L

L

S

S

K

K

R
|
R

P

P

M

M

G

D

R

R

V

V

I

T

D

L

P

P

L

L

S

K

R

K

M

M

G

G

A

V

D

S

F

I

T

S

S

G

S

Q

P

T

G

E

G

R

R

D

L

L

-

P

P

P

L

L

T

R

M

L

R

K

G

G

L

T

C

K

P

N

A

L

V

R

P

P

I

I

E

H

Y

Y

T

E

L

L

P

P

V

I

A

A

S
|
S

A
|
A

Q
|
Q

V

V

K

K

S

S

A

A

V

L

L

M

L

F

A

A

G

A

L

L

N

Q

T

A

P

K

G

G

I

E

T

S

R

V

V

I

I

I

E

E

P

K

V

E

L

Y

T

T

R
|
R

D

N

H

H

S

T

E

E

R

D

M

M

L

L

A

Q

G

Q

F

F

G

G

A

G

N

H

L

L

T

S

V

V

E

D

E

-

T

-

P

-

Q

-

G

G

R

K

I

K

I

I

S

T

I

V

H

Q

G

G

E

P

A

Q

E

K

F

L

K

T

P

G

Q

Q

Q

K

I

V

T

V

V

V

P

P

G

G

D

D

P

I

S

S

A

A

F

F

W

W

M

L

V

V

A

A

A

G

S

L

I

I

V

A

P

P

G

N

S

S

D

R

I

L

T

V

V

L

A

Q

N

N

V

V

G

G

L

I

N

N

P

E

T

T

R

R

I

T

G

G

L

I

I

I

T

D

A

V

L

I

R

R

M

A

M

M

G

G

A

G

-

K

-

L

D

E

I

I

T

T

E

E

V

I

N

D

P

P

R

V

I

-

V

-

G

-

E

A

P

K

V

S

A

A

D

T

L

L

R

I

V

V

R

E

H

S

A

S

P

D

L

L

S

K

A

G

I

T

E

E

V

I

P

C

H

G

D

A

L

L

A

I

P

P

S

R

M

L

I

I

D
|
D

E

E

Y

L

P

P

V

I

L

I

F

A

V

L

A

L

A

A

A

T

F

Q

A

A

V

Q

G

G

R

V

T

T

I

V

A

I

R

K

G

D

A

A

E

E

L

L

R

K

V

V

K
|
K

E
|
E

S

T

D

D

R
|
R

I

I

A

Q

A

V

M

V

V

A

A

D

A

A

L

L

A

N

P

S

N

M

G

G

V

A

P

D

L

I

E

T

E

P

F

T

E

A

D

D

G

G

L

M

A

I

I

I

T

K

G

G

T

K

G

S

G

A

G

-

P

-

I

-

A

L

G

H

G

G

A

A

P

R

V

V

A

N

S

T

K

F

L

G

D

D

H
|
H

R
|
R

I

I

A

G

M

M

S

M

M

T

A

A

V

I

A

A

G

A

-

L

L

L

G

V

A

A

K

D

A

G

D

E

V

V

S

E

I

L

D

D

D

R

V

A

S

E

P

A

V

I

A

N

T
|
T

S

S

Y

Y

P

P

G

S

F

F

S

D

T

D

L

L

D

E

S

S

L

L

active site: K20 (= K27), S21 (= S28), D47 (= D54), N90 (= N98), D115 (≠ A123), R120 (= R128), D312 (= D325), E340 (= E353), H384 (= H400), R385 (= R401), T412 (= T427)
binding (3r,4s,5r)-5-{[(1r)-1-carboxy-2-fluoro-1-(phosphonooxy)ethyl]oxy}-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid: K20 (= K27), S21 (= S28), R25 (= R32), G92 (= G100), T93 (= T101), R120 (= R128), S166 (= S173), A167 (= A174), Q168 (= Q175), R193 (= R200), D312 (= D325), K339 (= K352), E340 (= E353), R343 (= R356), H384 (= H400), R385 (= R401)

Q9S400 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) (see paper)
44% identity, 95% coverage: 17:439/443 of query aligns to 10:424/427 of Q9S400

query
sites
Q9S400

L

L

R

H

G

G

T

S

L

I

T

R

V

V

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

S

S

L

I

M

I

F

F

A

G

G

S

L

L

A

A

V

E

G

G

E

E

S

T

R

K

I

V

E

Y

G

D

L

I

L

L

E

R

G

G

E

E

D

D

V

V

L

L

A

S

T

T

A

M

A

Q

A

V

M

F

R

R

A

D

M

L

G

G

A

V

K

E

I

I

E

E

R

-

D

D

G

K

D

D

G

G

V

V

W

I

H

T

V

I

W

Q

G

G

V

V

G

G

V

M

G

A

G

G

L

L

L

K

Q

A

P

P

E

Q

T

N

A

A

L

L

E

N

M

M

G

G

N

N

S

S

G

G

T

T

S

S

T

I

R

R

L

L

L

I

M

S

G

G

L

V

V

L

A

A

S

G

H

A

P

D

I

F

T

E

A

V

T

E

F

M

T

F

G

G

D

D

A

D

S

S

L

L

S

S

K

K

R

R

P

P

M

M

G

D

R

R

V

V

I

T

D

L

P

P

L

L

S

K

R

K

M

M

G

G

A

V

D

S

F

I

T

S

S

G

S

Q

P

T

G

E

G

R

R

D

L

L

-

P

P

P

L

L

T

R

M

L

R

K

G

G

L

T

C

K

P

N

A

L

V

R

P

P

I

I

E

H

Y

Y

T

E

L

L

P

P

V

I

A

A

S

S

A

A

Q

Q

V

V

K

K

S

S

A

A

V

L

L

M

L

F

A

A

G

A

L

L

N

Q

T

A

P

K

G

G

I

E

T

S

R

V

V

I

I

I

E

E

P

K

V

E

L

Y

T

T

R

R

D

N

H

H

S

T

E

E

R

D

M

M

L

L

A

K

G

Q

F

F

G

G

A

G

N

H

L

L

T

S

V

V

E

D

E

-

T

-

P

-

Q

-

G

G

R

K

I

K

I

I

S

T

I

V

H

Q

G

G

E

P

A

Q

E

K

F

L

K

T

P

G

Q

Q

Q

K

I

V

T

V

V

V

P

P

G

G

D

D

P

I

S

S

A

A

F

F

W

W

M

L

V

V

A

A

A

G

S

L

I

I

V

A

P

P

G

N

S

S

D

R

I

L

T

V

V

L

A

Q

N

N

V

V

G

G

L

I

N

N

P

E

T

T

R

R

I

T

G

G

L

I

I

I

T

D

A

V

L

I

R

R

M

A

M

M

G

G

A

G

-

K

-

L

D

E

I

I

T

T

E

E

V

I

N

D

P

P

R

V

I

-

V

-

G

-

E

A

P

K

V

S

A

A

D

T

L

L

R

I

V

V

R

E

H

S

A

S

P

D

L

L

S

K

A

G

I

T

E

E

V

I

P

G

H

G

D

A

L

L

A

I

P

P

S

R

M

L

I

I

D

D

E

E

Y

L

P

P

V

I

L

I

F

A

V

L

A

L

A

A

A

T

F

Q

A

A

V

Q

G

G

R

V

T

T

I

V

A

I

R

K

G

D

A

A

E

E

L

L

R

K

V

V

K
|
K

E

E

S

T

D

D

R

R

I

I

A

Q

A

V

M

V

V

A

A

D

A

A

L

L

A

N

P

S

N

M

G

G

V

A

P

D

L

I

E

T

E

P

F

T

E

A

D

D

G

G

L

M

A

I

I

I

T

K

G

G

T

K

G

S

G

A

G

-

P

-

I

-

A

L

G

H

G

G

A

A

P

R

V

V

A

N

S

T

K

F

L

G

D

D

H

H

R

R

I

I

A

G

M

M

S

M

M

T

A

A

V

I

A

A

G

A

-

L

L

L

G

V

A

A

K

D

A

G

D

E

V

V

S

E

I

L

D

D

D

R

V

A

S

E

P

A

V

I

A

N

T

T

S

S

Y

Y

P

P

G

S

F

F

S

D

T

D

L

L

D

E

S

S

L

L

KS 20:21 (= KS 27:28) binding
R25 (= R32) binding
K339 (= K352) binding

7tm6A Crystal structure of shikimate-3-phosphate and glyphosate bound 3- phosphoshikimate 1-carboxyvinyltransferase from klebsiella pneumoniae
29% identity, 95% coverage: 17:436/443 of query aligns to 11:419/426 of 7tm6A

query
sites
7tm6A

L

V

R

E

G

G

T

T

L

V

T

N

V

L

P

P

G

G

D

S

K
|
K

S
|
S

I

V

S

S

H

N

R
|
R

S

A

L

L

M

L

F

L

A

A

G

A

L

L

A

A

V

R

G

G

E

T

S

T

R

V

I

L

E

T

G

N

L

L

E

D

G

S

E

D

D

D

V

V

L

R

A

H

T

M

A

L

A

N

A

A

M

L

R

S

A

A

M

L

G

G

A

V

K

Q

I

Y

E

T

R

L

D

S

G

A

D

D

G

R

V

T

W

-

H

R

V

C

W

E

G

V

V

T

G

G

V

N

G

G

L

P

L

L

Q

R

P

S

E

A

T

A

A

A

L

L

E

E

-

L

-

F

M

L

G

G

N

N

S

A

G
|
G

T
|
T

S

A

T

M

R

R

L

P

L

L

M

A

G

A

L

A

V

L

A

C

S

L

H

G

P

S

I

N

T

D

A

I

T

V

F

L

T

T

G

G

D

E

A

P

S

R

L

M

S

K

K

E

R
|
R

P

P

M

I

G

G

R

H

V

L

I

V

D

D

P

A

L

L

S

R

R

Q

M

G

G

G

A

A

D

Q

F

I

T

D

S

C

S

L

P

E

G

Q

G

E

R

N

L

Y

-

P

P

P

L

L

T

R

M

L

R

R

G

G

L

G

C

F

P

Q

A

G

V

G

P

N

I

V

E

E

Y

V

T

D

L

G

P

S

V

V

A

-

S
|
S

A
x
S

Q
|
Q

V

F

K

L

S

T

A

A

V

L

L

L

L

M

A

T

G

A

L

P

N

L

T

A

P

P

G

Q

I

D

T

T

R

V

V

I

I

V

-

I

-

K

-

G

-

D

-

L

-

V

-
x
S

E

K

P

P

V
x
Y

L

I

T

-

R

-

D

D

H

I

S

T

E

L

R

H

M

L

L

M

A

K

G

T

F

F

G

G

A

-

N

-

L

V

T

E

V

V

E

D

E

N

T

Q

P

S

Q

Y

G

Q

R

R

I

F

I

V

S

-

I

V

H

R

G

G

E

K

A

Q

E

Q

F

Y

K

Q

-

S

P

P

Q

G

Q

D

I

Y

T

L

V

V

P

E

G

G

D

D

P

A

S

S

A

A

A

S

F

Y

W

F

M

L

V

A

A

A

A

G

S

A

I

I

V

-

P

K

G

G

S

G

D

T

I

V

T

K

V

V

A

T

N

G

V

I

G

G

L

R

N

N

P

S

T

V

R

Q

-

G

-

D

I

I

G

R

L

F

I

A

T

D

A

V

L

L

R

E

M

K

M

M

G

G

A

A

D

T

I

V

T

T

E

-

V

-

N

-

P

-

R

-

I

-

V

W

G

G

G

D

E

D

P

F

V

I

A

A

D

-

L

-

R

-

V

C

R

T

H

H

A

G

P

E

L

L

S

K

A

A

I

V

E

D

V

M

P

D

H

M

D

N

L

H

A

I

P

P

S

-

M

-

I

-

D
|
D

E

A

Y

A

P

M

V

T

L

I

F

A

V

T

A

A

A

L

F

F

A

A

V

Q

G

G

R

T

T

T

I

T

A

L

R

R

G

N

A

I

E

Y

E
x
N

L

W

R

R

V

V

K
|
K

E
|
E

S

T

D

D

R
|
R

I

L

A

F

A

A

M

M

V

A

A

T

A

E

L

L

A

R

P

K

N

V

G

G

V

A

P

E

L

V

E

E

F

G

E

E

D

D

G

Y

L

I

A

R

I

I

T

T

G

-

T

-

G

-

P

P

I

P

A

A

-

K

-

L

G

K

G

Y

A

A

P

E

V

I

A

G

S

T

K

Y

L

N

D

D

H
|
H

R
|
R

I

M

A

A

M

M

S

C

M

F

A

S

V

L

A

V

G

A

L

L

G

-

A

S

K

D

A

T

D

P

V

V

S

T

I

I

D

L

D

D

V

P

S

K

P

C

V

T

A

A

T

K

S

T

Y

F

P

P

G

D

F

Y

F

F

S

E

T

Q

L

L

binding glyphosate: K21 (= K27), G95 (= G100), R123 (= R128), Q170 (= Q175), D312 (= D325), E340 (= E353), R343 (= R356), H384 (= H400), R385 (= R401)
binding shikimate-3-phosphate: S22 (= S28), R26 (= R32), T96 (= T101), S168 (= S173), S169 (≠ A174), Q170 (= Q175), S196 (vs. gap), Y199 (≠ V197), D312 (= D325), N335 (≠ E348), K339 (= K352)

7tm5B Crystal structure of shikimate-3-phosphate bound 3-phosphoshikimate 1- carboxyvinyltransferase from klebsiella pneumoniae
29% identity, 95% coverage: 17:436/443 of query aligns to 12:420/427 of 7tm5B

query
sites
7tm5B

L

V

R

E

G

G

T

T

L

V

T

N

V

L

P

P

G

G

D

S

K
|
K

S
|
S

I

V

S

S

H

N

R
|
R

S

A

L

L

M

L

F

L

A

A

G

A

L

L

A

A

V

R

G

G

E

T

S

T

R

V

I

L

E

T

G

N

L

L

E

D

G

S

E

D

D

D

V

V

L

R

A

H

T

M

A

L

A

N

A

A

M

L

R

S

A

A

M

L

G

G

A

V

K

Q

I

Y

E

T

R

L

D

S

G

A

D

D

G

R

V

T

W

-

H

R

V

C

W

E

G

V

V

T

G

G

V

N

G

G

L

P

L

L

Q

R

P

S

E

A

T

A

A

A

L

L

E

E

-

L

-

F

M

L

G

G

N

N

S

A

G

G

T

T

S

A

T

M

R

R

L

P

L

L

M

A

G

A

L

A

V

L

A

C

S

L

H

G

P

S

I

N

T

D

A

I

T

V

F

L

T

T

G

G

D

E

A

P

S

R

L

M

S

K

K

E

R

R

P

P

M

I

G

G

R

H

V

L

I

V

D

D

P

A

L

L

S

R

R

Q

M

G

G

G

A

A

D

Q

F

I

T

D

S

C

S

L

P

E

G

Q

G

E

R

N

L

Y

-

P

P

P

L

L

T

R

M

L

R

R

G

G

L

G

C

F

P

Q

A

G

V

G

P

N

I

V

E

E

Y

V

T

D

L

G

P

S

V

V

A

-

S
|
S

A
x
S

Q
|
Q

V

F

K

L

S

T

A

A

V

L

L

L

L

M

A

T

G

A

L

P

N

L

T

A

P

P

G

Q

I

D

T

T

R

V

V

I

I

V

-

I

-

K

-

G

-

D

-

L

-

V

-
x
S

E

K

P

P

V
x
Y

L

I

T

-

R

-

D

D

H

I

S

T

E

L

R

H

M

L

L

M

A

K

G

T

F

F

G

G

A

-

N

-

L

V

T

E

V

V

E

D

E

N

T

Q

P

S

Q

Y

G

Q

R

R

I

F

I

V

S

-

I

V

H

R

G

G

E

K

A

Q

E

Q

F

Y

K

Q

-

S

P

P

Q

G

Q

D

I

Y

T

L

V

V

P

E

G

G

D

D

P

A

S

S

A

A

A

S

F

Y

W

F

M

L

V

A

A

A

A

G

S

A

I

I

V

-

P

K

G

G

S

G

D

T

I

V

T

K

V

V

A

T

N

G

V

I

G

G

L

R

N

N

P

S

T

V

R

Q

-

G

-

D

I

I

G

R

L

F

I

A

T

D

A

V

L

L

R

E

M

K

M

M

G

G

A

A

D

T

I

V

T

T

E

-

V

-

N

-

P

-

R

-

I

-

V

W

G

G

G

D

E

D

P

F

V

I

A

A

D

-

L

-

R

-

V

C

R

T

H

H

A

G

P

E

L

L

S

K

A

A

I

V

E

D

V

M

P

D

H

M

D

N

L

H

A

I

P

P

S

-

M

-

I

-

D
|
D

E

A

Y

A

P

M

V

T

L

I

F

A

V

T

A

A

A

L

F

F

A

A

V

Q

G

G

R

T

T

T

I

T

A

L

R

R

G

N

A

I

E

Y

E
x
N

L

W

R

R

V

V

K
|
K

E

E

S

T

D

D

R

R

I

L

A

F

A

A

M

M

V

A

A

T

A

E

L

L

A

R

P

K

N

V

G

G

V

A

P

E

L

V

E

E

F

G

E

E

D

D

G

Y

L

I

A

R

I

I

T

T

G

-

T

-

G

-

P

P

I

P

A

A

-

K

-

L

G

K

G

Y

A

A

P

E

V

I

A

G

S

T

K

Y

L

N

D

D

H

H

R

R

I

M

A

A

M

M

S

C

M

F

A

S

V

L

A

V

G

A

L

L

G

-

A

S

K

D

A

T

D

P

V

V

S

T

I

I

D

L

D

D

V

P

S

K

P

C

V

T

A

A

T

K

S

T

Y

F

P

P

G

D

F

Y

F

F

S

E

T

Q

L

L

binding shikimate-3-phosphate: K22 (= K27), S23 (= S28), R27 (= R32), S169 (= S173), S170 (≠ A174), Q171 (= Q175), S197 (vs. gap), Y200 (≠ V197), D313 (= D325), N336 (≠ E348), K340 (= K352)

6hqvA Pentafunctional arom complex from chaetomium thermophilum (see paper)
31% identity, 95% coverage: 20:439/443 of query aligns to 401:827/1555 of 6hqvA

query
sites
6hqvA

T

T

L

V

T

T

V

P

P

P

G

G

D

S

K

K

S

S

I

I

S

S

H

N

R
|
R

S

A

L

L

M

V

F

L

A

A

G

A

L

L

A

G

V

E

G

G

E

T

S

T

R

R

I

I

E

H

G

G

L

L

E

H

G

S

E

D

D

D

V

V

L

Q

A

Y

T

M

A

L

A

A

M

I

R

E

A

Q

M

L

G

H

A

G

K

-

I

-

E

-

R

-

D

-

G

A

D

D

G

F

V

S

W

W

H

E

V

D

W

A

G

G

-

E

-

I

-

L

-

V

V

T

G

G

V

K

G

G

L

K

L

L

Q

Q

-

A

P

S

E

K

T

E

A

P

L

L

E

Y

M

L

G

G

N

N

S

A

G

G

T

T

S

A

T

S

R

R

L

F

L

L

M

T

G

S

L

V

V

V

A

A

-

L

-

C

-

A

-

P

S

S

H

A

P

V

I

S

T

S

A

T

T

V

F

L

T

T

G

G

D

N

A

A

S

R

L

M

S

K

K

V

R

R

P

P

M

I

G

G

R

A

V

L

I

V

D

D

P

A

L

L

S

R

R

A

M

N

G

G

A

V

D

G

F

V

T

K

S

Y

S

L

P

E

G

K

G

E

R

K

-

S

L

L

P

P

L

V

T

E

M

V

R

D

-

A

-

G

G

G

L

F

C

A

P

G

A

G

V

V

P

-

I

I

E

E

Y

L

T

A

L

A

P

T

V

V

A

S

S
|
S

A
x
Q

Q

Y

V

V

K

S

S

S

A

I

V

L

L

M

L

A

A

A

G

P

L

Y

-

A

N

H

T

Q

P

P

G

V

I

T

T

L

R

R

V

L

I

V

-

G

-

K

-

P

-

I

-

S

E

Q

P

P

V

Y

L

I

T

-

R

-

D

D

H

M

S

T

E

I

R

A

M

M

L

M

A

A

G

S

F

F

G

G

A

-

N

-

L

I

T

K

V

V

E

E

E

R

T

S

P

A

Q

E

G

D

R

P

I

N

I

T

S

Y

I

L

H

I

G

P

E

K

A

G

E

V

F

Y

K

K

-

N

P

P

Q

P

Q

E

I

Y

T

V

V

V

P

E

G

S

D

D

P

A

S

S

A

A

A

T

F

Y

W

P

M

L

V

A

A

V

A

A

S

A

I

I

V

T

P

-

G

G

S

T

D

T

I

C

T

T

V

I

A

P

N

N

V

I

G

G

-

S

-

E

-

S

L

L

N

Q

P

G

T

D

R

A

I

R

G

F

L

A

I

V

T

E

A

V

L

L

R

R

M

P

M

M

G

G

A

C

D

A

I

V

T

E

E

Q

V

T

-

A

N

T

P

S

R

T

I

T

V

V

G

T

G

G

E

P

P

P

V

I

A

G

D

T

L

L

R

K

V

A

R

-

H

-

A

-

P

-

L

-

S

-

A

-

I

-

E

-

V

I

P

P

H

H

-

V

D

D

L

M

A

E

P

P

S

-

M

M

I

T

D

D

E

A

Y

F

P

L

V

T

L

A

F

A

V

V

A

L

A

A

F

V

A

A

V

D

G

G

R

T

T

T

I

Q

A

I

R

T

G

G

A

I

E

A

E

N

L

Q

R

R

V

V

K

K

E

E

S

C

D

N

R

R

I

I

A

A

M

M

V

K

A

D

A

Q

L

L

A

A

P

K

N

F

G

G

V

V

P

Q

L

C

E

N

E

E

F

L

E

E

D

D

G

G

L

I

A

E

I

V

T

I

G

G

T

K

G

P

G

Y

-

Q

-

E

-

L

-

R

G

N

P

P

I

V

A

E

G

G

G

-

A

-

P

-

V

I

A

Y

S

C

K

Y

L

D

D

D

H

H

R

R

I

V

A

A

M

M

S

S

M

H

A

S

V

V

A

L

G

S

L

T

G

I

A

S

K

P

A

H

D

P

V

V

S

L

I

I

D

L

D

E

V

R

S

E

P

C

V

T

A

A

T

K

S

T

Y

W

P

P

G

G

F

W

S

D

T

I

L

L

D

S

S

Q

L

F

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: R413 (= R32), S562 (= S173), Q563 (≠ A174)

Sites not aligning to the query:

active site: 123, 145, 187, 243, 253, 257, 261, 264, 268, 280
binding (4S,5R)-4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylic acid: 1060, 1062, 1181, 1224, 1232, 1242, 1243
binding glutamic acid: 139, 145, 187, 243, 257, 264, 280
binding nicotinamide-adenine-dinucleotide: 42, 44, 45, 76, 79, 107, 108, 109, 112, 132, 133, 135, 139, 140, 145, 154, 175, 176, 177, 180, 280
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 874, 923, 924, 979, 1277, 1279, 1323, 1327, 1348, 1368, 1526
binding zinc ion: 187, 264, 280

P11043 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EC 2.5.1.19 from Petunia hybrida (Petunia) (see paper)
27% identity, 96% coverage: 17:440/443 of query aligns to 85:514/516 of P11043

query
sites
P11043

L

I

R

S

G

G

T

T

L

V

T

K

V

L

P

P

G

G

D

S

K

K

S

S

I

L

S

S

H

N

R

R

S

I

L

L

M

L

F

L

A

A

G

A

L

L

A

S

V

E

G

G

E

T

S

T

R

V

I

V

E

D

G

N

L

L

E

S

G

S

E

D

D

D

V

I

L

H

A

Y

T

M

A

L

A

G

A

A

M

L

R

K

A

T

M

L

G

G

A

L

K

H

I

V

E

E

R

E

D

D

G

S

D

A

G

N

V

Q

W

R

H

A

V

V

W

V

G

E

V

-

G

G

V

C

G

G

L

L

L

F

-

P

-

V

-

G

-

K

-

E

-

S

Q

K

P

E

E

E

T

I

A

Q

L

L

E

F

M

L

G

G

N

N

S

A

G
|
G

T

T

S

A

T

M

R

R

L

P

L

L

M

T

G

A

L

A

V

V

A

T

S

V

H

A

P

G

I

G

T

N

A

S

T

R

F

Y

T

V

G

L

D

D

A

G

-

V

-

P

S

R

L

M

S

R

K

E

R

R

P

P

M

I

G

S

R

D

V

L

I

V

D

D

P

G

L

L

S

K

R

Q

M

L

G

G

A

A

D

E

F

V

T

D

S

C

S

F

P

L

G

G

G

T

R

K

L

C

P

P

-

P

-

V

-

R

L

I

T

V

M

S

R

K

G

G

L

G

C

L

P

P

A

G

V

G

P

K

I

V

E

K

Y

L

T

S

L

G

P

S

V

I

A

S

S

S

A

Q

Q

Y

V

L

K

T

S

A

A

L

V

L

L

M

L

A

A

A

G

P

L

L

N

A

T

L

P

G

G

D

I

V

T

E

-

I

R

E

V

I

I

I

E

D

P

K

V

L

L

I

T

S

R

V

D

P

H

Y

S

V

E

E

-

M

-

T

-

L

R

K

M

L

L

M

A

E

G

R

F

F

G

G

A

-

N

-

L

I

T

S

V

V

E

E

E

H

T

S

P

S

Q

S

G

W

R

D

I

R

I

F

S

F

I

V

H

R

G

G

E

G

A

Q

E

K

F

Y

K

K

-

S

P

P

Q

G

Q

K

I

A

T

F

V

V

P

E

G

G

D

D

P

A

S

S

A

A

A

S

F

Y

W

F

M

L

V

A

A

G

A

A

S

A

I

V

V

T

P

G

G

G

S

T

D

-

I

I

T

T

V

V

A

E

N

G

V

C

G

G

L

T

N

N

P

S

T

L

R

Q

-

G

-

D

I

V

G

K

L

F

I

A

T

E

A

V

L

L

R

E

M

K

M

M

G

G

A

A

D

E

I

V

T

T

E

W

V

T

-

E

N

N

P

S

R

V

I

T

V

V

G

K

G

G

E

P

P

P

V

R

A

S

D

S

L

S

R

G

V

R

R

K

H

H

A

-

P

-

L

L

S

R

A

A

I

I

E

D

V

V

P

N

H

M

D

N

L

K

A

M

P

P

S

-

M

-

I

-

D

D

E

V

Y

A

P

M

V

T

L

L

F

A

V

V

A

V

A

A

A

L

F

Y

A

A

V

D

G

G

R

P

T

T

I

A

A

I

R

R

G

D

A

V

E

A

E

S

L

W

R

R

V

V

K

K

E

E

S

T

D

E

R

R

I

M

A

I

A

A

M

I

V

C

A

T

A

E

L

L

A

R

P

K

N

L

G

G

V

A

P

T

L

V

E

E

F

G

E

P

D

D

G

Y

L

C

A

I

I

I

T

T

G

P

T

P

G

E

G

K

G

L

P

N

I

V

A

T

G

D

G

-

A

-

P

-

V

I

A

D

S

T

K

Y

L

D

D

D

H

H

R

R

I

M

A

A

M

M

S

A

M

F

A

S

V

L

A

A

G

A

L

C

G

-

A

A

K

D

A

V

D

P

V

V

S

T

I

I

D

N

D

D

V

P

S

G

P

C

V

T

A

R

T

K

S

T

Y

F

P

P

G

N

F

Y

F

F

S

D

T

V

L

L

D

Q

S

Q

L

Y

T

S

G173 (= G100) mutation to A: Resistance to glyphosate due to a lower affinity. Slight reduction in EPSP synthase activity.

3nvsA 1.02 angstrom resolution crystal structure of 3-phosphoshikimate 1- carboxyvinyltransferase from vibrio cholerae in complex with shikimate-3-phosphate (partially photolyzed) and glyphosate
27% identity, 96% coverage: 17:440/443 of query aligns to 12:425/426 of 3nvsA

query
sites
3nvsA

L

I

R

S

G

G

T

E

L

V

T

N

V

L

P

P

G

G

D

S

K
|
K

S
|
S

I

V

S

S

H

N

R
|
R

S

A

L

L

M

L

F

L

A

A

G

A

L

L

A

A

V

S

G

G

E

T

S

T

R

R

I

L

E

T

G

N

L

L

E

D

G

S

E

D

D
|
D

V

I

L

R

A

H

T

M

A

L

A

N

A

A

M

L

R

T

A

K

M

L

G

G

A

V

K

N

I

Y

E

R

R

L

D

S

G

A

D

D

G

K

-

T

V

T

W

C

H

E

V

V

W

E

G

G

V

L

G

G

V

Q

G

A

G

F

L

H

L

T

Q

T

P

Q

E

P

T

L

A

E

L

L

E

F

M

L

G

G

N
|
N

S

A

G
|
G

T
|
T

S

A

T

M

R

R

L

P

L

L

M

A

G

A

L

A

V

L

A

C

S

L

H

G

P

Q

I

G

T

D

A

Y

T

V

F

L

T

T

G

G

D

E

A
x
P

S

R

L

M

S

K

K
x
E

R
|
R

P

P

M

I

G

G

R
x
H

V

L

I

V

D

D

P

A

L

L

S

R

R
x
Q

M

A

G

G

A

A

D

Q

F

I

T

E

S
x
Y

S

L

P
x
E

G
x
Q

G

E

R

N

L

F

-

P

P

P

L

L

T

R

M

I

R

Q

G

G

L

T

-

G

-

L

-

Q

C

A

P

G

A

T

V

V

P

T

I

I

E

D

Y

G

T

S

L

I

P

-

V

-

A

-

S
|
S

A
x
S

Q
|
Q

V

F

K

L

S

T

A

A

V

F

L

L

L

M

A

S

G

A

-

P

L

L

N

A

T

Q

P

G

G

K

I

V

T

T

-

I

R

K

V

I

I

V

E

G

P

E

V

L

L

V

T
x
S

R

K

-

P

-
x
Y

-

I

D

D

H

I

S

T

E

L

R

H

M

I

L

M

A

E

G

Q

F

F

G

G

A

V

N

Q

L

V

T

I

V

N

E

H

E

D

T

Y

P

Q

Q

E

G

F

R

V

I

I

I

P

S

A

I

-

H

-

G

G

E

Q

A

S

E

Y

F

V

K

S

P

P

Q

G

Q

Q

I

F

T

L

V

V

P

E

G

G

D

D

P

A

S

S

A
|
A

A

S

F

Y

W

F

M

L

V

A

A

A

S
x
A

I

I

V

-

P

K

G

G

S

G

D

E

I

V

T

K

V

V

A

T

N

G

V

I

G

G

L

K

N

N

P

S

T

I

R

Q

-

G

-

D

I

I

G

Q

L

F

I

A

T

D

A

A

L

L

R

E

M

K

M

M

G

G

A

A

D

Q

I

I

T

E

E

W

V

G

N

D

P

D

R

Y

I

V

V

I

G

A

G

-

E

-

P

-

V

-

A

-

D

-

L

-

R

-

V

-

R

R

H

R

A

G

P

E

L

L

S

N

A

A

I

V

E

D

V

L

P

D

H

F

D

N

L

H

A

I

P

P

S

-

M

-

I

-

D
|
D

E

A

Y

A

P

M

V

T

L

I

F

A

V

T

A

T

A

A

A

L

F

F

A

A

V

K

G

G

R

T

T

T

I

A

A

I

R

R

G

N

A

V

E

Y

E
x
N

L

W

R

R

V

V

K
|
K

E
|
E

S

T

D

D

R
|
R

I

L

A

A

M

M

V

A

A

T

A

E

L

L

A

R

P

K

N

V

G

G

V

A

P

T

L

V

E

E

F

G

E

E

D

D

G

F

L

I

A

V

I

I

T

T

G

-

T

-

G

-

P

P

I

P

A

T

G

K

-

L

-

I

G

H

A

A

P

A

V

I

A

D

S

T

K

Y

L

D

D

D

H
|
H

R
|
R

I

M

A

A

M

M

S

C

M

F

A

S

V

L

A

V

G

A

L

L

G

-

A

S

K

D

A

T

D

P

V

V

S

T

I

I

D

N

D

D

V

P

S

K

P

C

V

T

A

S

T
x
K

S

T

Y

F

P

P

G

D

F

Y

F

F

S

D

T

K

L

F

D

A

S

Q

L

L

T

S

active site: K22 (= K27), S23 (= S28), D49 (= D54), N94 (= N98), P119 (≠ A123), R124 (= R128), H128 (≠ R132), Q135 (≠ R139), Y142 (≠ S146), E144 (≠ P148), A247 (= A247), A255 (≠ S255), D314 (= D325), E342 (= E353), H386 (= H400), R387 (= R401), K412 (≠ T427)
binding glyphosate: K22 (= K27), G96 (= G100), R124 (= R128), Q172 (= Q175), D314 (= D325), E342 (= E353), R345 (= R356), H386 (= H400), R387 (= R401)
binding magnesium ion: E123 (≠ K127), Q145 (≠ G149)
binding shikimate-3-phosphate: K22 (= K27), S23 (= S28), R27 (= R32), T97 (= T101), S170 (= S173), S171 (≠ A174), Q172 (= Q175), S198 (≠ T199), Y201 (vs. gap), D314 (= D325), N337 (≠ E348), K341 (= K352)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S23 (= S28), R27 (= R32), Q172 (= Q175), Y201 (vs. gap), D314 (= D325), K341 (= K352)

Q9KRB0 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
27% identity, 96% coverage: 17:440/443 of query aligns to 12:425/426 of Q9KRB0

query
sites
Q9KRB0

L

I

R

S

G

G

T

E

L

V

T

N

V

L

P

P

G

G

D

S

K
|
K

S
|
S

I

V

S

S

H

N

R
|
R

S

A

L

L

M

L

F

L

A

A

G

A

L

L

A

A

V

S

G

G

E

T

S

T

R

R

I

L

E

T

G

N

L

L

E

D

G

S

E

D

D

D

V

I

L

R

A

H

T

M

A

L

A

N

A

A

M

L

R

T

A

K

M

L

G

G

A

V

K

N

I

Y

E

R

R

L

D

S

G

A

D

D

G

K

-

T

V

T

W

C

H

E

V

V

W

E

G

G

V

L

G

G

V

Q

G

A

G

F

L

H

L

T

Q

T

P

Q

E

P

T

L

A

E

L

L

E

F

M

L

G

G

N
|
N

S
x
A

G
|
G

T
|
T

S

A

T

M

R

R

L

P

L

L

M

A

G

A

L

A

V

L

A

C

S

L

H

G

P

Q

I

G

T

D

A

Y

T

V

F

L

T

T

G

G

D

E

A

P

S

R

L

M

S

K

K

E

R
|
R

P

P

M

I

G

G

R

H

V

L

I

V

D

D

P

A

L

L

S

R

R

Q

M

A

G

G

A

A

D

Q

F

I

T

E

S

Y

S

L

P

E

G

Q

G

E

R

N

L

F

-

P

P

P

L

L

T

R

M

I

R

Q

G

G

L

T

-

G

-

L

-

Q

C

A

P

G

A

T

V

V

P

T

I

I

E

D

Y

G

T

S

L

I

P

-

V

-

A

-

S
|
S

A
x
S

Q
|
Q

V

F

K

L

S

T

A

A

V

F

L

L

L

M

A

S

G

A

-

P

L

L

N

A

T

Q

P

G

G

K

I

V

T

T

-

I

R

K

V

I

I

V

E

G

P

E

V

L

L

V

T
x
S

R

K

-

P

-

Y

-

I

D

D

H

I

S

T

E

L

R

H

M

I

L

M

A

E

G

Q

F

F

G

G

A

V

N

Q

L

V

T

I

V

N

E

H

E

D

T

Y

P

Q

Q

E

G

F

R

V

I

I

I

P

S

A

I

-

H

-

G

G

E

Q

A

S

E

Y

F

V

K

S

P

P

Q

G

Q

Q

I

F

T

L

V

V

P

E

G

G

D

D

P

A

S

S

A

A

A

S

F

Y

W

F

M

L

V

A

A

A

S

A

I

I

V

-

P

K

G

G

S

G

D

E

I

V

T

K

V

V

A

T

N

G

V

I

G

G

L

K

N

N

P

S

T

I

R

Q

-

G

-

D

I

I

G

Q

L

F

I

A

T

D

A

A

L

L

R

E

M

K

M

M

G

G

A

A

D

Q

I

I

T

E

E

W

V

G

N

D

P

D

R

Y

I

V

V

I

G

A

G

-

E

-

P

-

V

-

A

-

D

-

L

-

R

-

V

-

R

R

H

R

A

G

P

E

L

L

S

N

A

A

I

V

E

D

V

L

P

D

H

F

D

N

L

H

A

I

P

P

S

-

M

-

I

-

D

D

E

A

Y

A

P

M

V

T

L

I

F

A

V

T

A

T

A

A

A

L

F

F

A

A

V

K

G

G

R

T

T

T

I

A

A

I

R

R

G

N

A

V

E

Y

E
x
N

L

W

R

R

V

V

K
|
K

E

E

S

T

D

D

R
|
R

I

L

A

A

M

M

V

A

A

T

A

E

L

L

A

R

P

K

N

V

G

G

V

A

P

T

L

V

E

E

F

G

E

E

D

D

G

F

L

I

A

V

I

I

T

T

G

-

T

-

G

-

P

P

I

P

A

T

G

K

-

L

-

I

G

H

A

A

P

A

V

I

A

D

S

T

K

Y

L

D

D

D

H

H

R
|
R

I

M

A

A

M

M

S

C

M

F

A

S

V

L

A

V

G

A

L

L

G

-

A

S

K

D

A

T

D

P

V

V

S

T

I

I

D

N

D

D

V

P

S

K

P

C

V

T

A

S

T

K

S

T

Y

F

P

P

G

D

F

Y

F

F

S

D

T

K

L

F

D

A

S

Q

L

L

T

S

KS 22:23 (= KS 27:28) binding
R27 (= R32) binding
NAGT 94:97 (≠ NSGT 98:101) Phosphoenolpyruvate
R124 (= R128) binding
SSQ 170:172 (≠ SAQ 173:175) binding
S198 (≠ T199) binding
N337 (≠ E348) binding
K341 (= K352) binding
R345 (= R356) binding
R387 (= R401) binding

2pq9A E. Coli epsps liganded with (r)-difluoromethyl tetrahedral reaction intermediate analog (see paper)
29% identity, 94% coverage: 19:436/443 of query aligns to 14:420/427 of 2pq9A

query
sites
2pq9A

G

G

T

T

L

I

T

N

V

L

P

P

G

G

D

S

K
|
K

S
|
S

I

V

S

S

H

N

R
|
R

S

A

L

L

M

L

F

L

A

A

G

A

L

L

A

A

V

H

G

G

E

K

S

T

R

V

I

L

E

T

G

N

L

L

E

D

G

S

E

D

D
|
D

V

V

L

R

A

H

T

M

A

L

A

N

A

A

M

L

R

T

A

A

M

L

G

G

A

V

K

S

I

Y

E

T

R

L

D

S

G

A

D

D

G

R

V

T

W

-

H

R

V

C

W

E

G

I

V

I

G

G

V

N

G

G

L

P

L

L

Q

H

P

A

E

E

T

G

A

A

L

L

E

E

-

L

-

F

M

L

G

G

N
|
N

S

A

G
|
G

T
|
T

S

A

T

M

R

R

L

P

L

L

M

A

G

A

L

A

V

L

A

C

S

L

H

G

P

S

I

N

T

D

A

I

T

V

F

L

T

T

G

G

D

E

A
x
P

S

R

L

M

S

K

K

E

R
|
R

P

P

M

I

G

G

R

H

V

L

I

V

D

D

P

A

L

L

S

R

R

L

M

G

G

G

A

A

D

K

F

I

T

T

S

Y

S

L

P

E

G

Q

G

E

R

N

L

Y

-

P

P

P

L

L

T

R

M

L

R

Q

G

G

L

G

C

F

P

T

A

G

V

G

P

N

I

V

E

D

Y

V

T

D

L

G

P

S

V

V

A

-

S
|
S

A
x
S

Q
|
Q

V

F

K

L

S

T

A

A

V

L

L

L

L

M

A

T

G

A

L

P

N

L

T

A

P

P

-

E

-

D

G

T

I

V

T

I

R

R

V

I

I

K

E

G

P

D

V

L

L

V

T
x
S

R

K

-

P

-
x
Y

-

I

D

D

H

I

S

T

E

L

R

N

M

L

L

M

A

K

G

T

F

F

G

G

A

-

N

-

L

V

T

E

V

I

E

E

E

N

T

Q

P

H

Q

Y

G

Q

R

Q

I

F

I

V

S

V

I

K

H

G

G

G

E

Q

A

S

E

Y

F

Q

K

S

P

P

Q

G

Q

T

I

Y

T

L

V

V

P

E

G

G

D

D

P

A

S

S

A

A

A

S

F

Y

W

F

M

L

V

A

A

A

S

A

I

I

V

-

P

K

G

G

S

G

D

T

I

V

T

K

V

V

A

T

N

G

V

I

G

G

L

R

N

N

P

S

T

M

R

Q

-

G

-

D

I

I

G

R

L

F

I

A

T

D

A

V

L

L

R

E

M

K

M

M

G

G

A

A

D

T

I

I

T

C

E

W

V

G

N

D

P

D

R

Y

I

I

-

S

-

C

V

T

G

R

G

G

E

E

P

L

V

N

A

A

-

I

D

D

L

M

R

D

V

M

R

N

H

H

A

I

P

P

L
x
D

S

A

A

A

I

M

E

T

V

I

P

A

H

-

D

-

L

-

A

-

P

-

S

-

M

-

I

-

D

-

E

-

Y

-

P

-

V

-

L

-

F

-

V

T

A

A

A

L

F

F

A

A

V

K

G

G

R

T

T

T

I

T

A

L

R

R

G

N

A

I

E

Y

E
x
N

L

W

R

R

V

V

K
|
K

E
|
E

S

T

D

D

R
|
R

I

L

A

F

A

A

M

M

V

A

A

T

A

E

L

L

A

R

P

K

N

V

G

G

V

A

P

E

L

V

E

E

F

G

E

H

D

D

G

Y

L

I

A

R

I

I

T

T

G

P

T

P

G

-

P

E

I

K

A

L

G

N

G

F

A

A

P

E

V

I

A

A

S

T

K

Y

L

N

D

D

H
|
H

R
|
R

I

M

A

A

M

M

S

C

M

F

A

S

V

L

A

V

G

A

L

L

G

-

A

S

K

D

A

T

D

P

V

V

S

T

I

I

D

L

D

D

V

P

S

K

P

C

V

T

A

A

T
x
K

S

T

Y

F

P

P

G

D

F

Y

F

F

S

E

T

Q

L

L

active site: K22 (= K27), S23 (= S28), D49 (= D54), N94 (= N98), P119 (≠ A123), R124 (= R128), D313 (≠ L310), E341 (= E353), H385 (= H400), R386 (= R401), K411 (≠ T427)
binding (3r,4s,5r)-5-[(1r)-1-carboxy-2,2-difluoro-1-(phosphonooxy)ethoxy]-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid: K22 (= K27), S23 (= S28), R27 (= R32), G96 (= G100), T97 (= T101), R124 (= R128), S169 (= S173), S170 (≠ A174), Q171 (= Q175), S197 (≠ T199), Y200 (vs. gap), D313 (≠ L310), N336 (≠ E348), K340 (= K352), R344 (= R356), H385 (= H400), R386 (= R401), K411 (≠ T427)

2aa9A Epsp synthase liganded with shikimate (see paper)
29% identity, 94% coverage: 19:436/443 of query aligns to 14:420/427 of 2aa9A

query
sites
2aa9A

G

G

T

T

L

I

T

N

V

L

P

P

G

G

D

S

K
|
K

S
|
S

I

V

S

S

H

N

R
|
R

S

A

L

L

M

L

F

L

A

A

G

A

L

L

A

A

V

H

G

G

E

K

S

T

R

V

I

L

E

T

G

N

L

L

E

D

G

S

E

D

D
|
D

V

V

L

R

A

H

T

M

A

L

A

N

A

A

M

L

R

T

A

A

M

L

G

G

A

V

K

S

I

Y

E

T

R

L

D

S

G

A

D

D

G

R

V

T

W

-

H

R

V

C

W

E

G

I

V

I

G

G

V

N

G

G

L

P

L

L

Q

H

P

A

E

E

T

G

A

A

L

L

E

E

-

L

-

F

M

L

G

G

N
|
N

S

A

G

G

T
|
T

S

A

T

M

R

R

L

P

L

L

M

A

G

A

L

A

V

L

A

C

S

L

H

G

P

S

I

N

T

D

A

I

T

V

F

L

T

T

G

G

D

E

A
x
P

S

R

L

M

S

K

K

E

R
|
R

P

P

M

I

G

G

R

H

V

L

I

V

D

D

P

A

L

L

S

R

R

L

M

G

G

G

A

A

D

K

F

I

T

T

S

Y

S

L

P

E

G

Q

G

E

R

N

L

Y

-

P

P

P

L

L

T

R

M

L

R

Q

G

G

L

G

C

F

P

T

A

G

V

G

P

N

I

V

E

D

Y

V

T

D

L

G

P

S

V

V

A

-

S

S

A

S

Q
|
Q

V

F

K

L

S

T

A

A

V

L

L

L

L

M

A

T

G

A

L

P

N

L

T

A

P

P

-

E

-

D

G

T

I

V

T

I

R

R

V

I

I

K

E

G

P

D

V

L

L

V

T

S

R

K

-

P

-
x
Y

-

I

D

D

H

I

S

T

E

L

R

N

M

L

L

M

A

K

G

T

F

F

G

G

A

-

N

-

L

V

T

E

V

I

E

E

E

N

T

Q

P

H

Q

Y

G

Q

R

Q

I

F

I

V

S

V

I

K

H

G

G

G

E

Q

A

S

E

Y

F

Q

K

S

P

P

Q

G

Q

T

I

Y

T

L

V

V

P

E

G

G

D

D

P

A

S

S

A

A

A

S

F

Y

W

F

M

L

V

A

A

A

S

A

I

I

V

-

P

K

G

G

S

G

D

T

I

V

T

K

V

V

A

T

N

G

V

I

G

G

L

R

N

N

P

S

T

M

R

Q

-

G

-

D

I

I

G

R

L

F

I

A

T

D

A

V

L

L

R

E

M

K

M

M

G

G

A

A

D

T

I

I

T

C

E

W

V

G

N

D

P

D

R

Y

I

I

-

S

-

C

V

T

G

R

G

G

E

E

P

L

V

N

A

A

-

I

D

D

L

M

R

D

V

M

R

N

H

H

A

I

P

P

L
x
D

S

A

A

A

I

M

E

T

V

I

P

A

H

-

D

-

L

-

A

-

P

-

S

-

M

-

I

-

D

-

E

-

Y

-

P

-

V

-

L

-

F

-

V

T

A

A

A

L

F

F

A

A

V

K

G

G

R

T

T

T

I

T

A

L

R

R

G

N

A

I

E

Y

E

N

L

W

R

R

V

V

K
|
K

E
|
E

S

T

D

D

R

R

I

L

A

F

A

A

M

M

V

A

A

T

A

E

L

L

A

R

P

K

N

V

G

G

V

A

P

E

L

V

E

E

F

G

E

H

D

D

G

Y

L

I

A

R

I

I

T

T

G

P

T

P

G

-

P

E

I

K

A

L

G

N

G

F

A

A

P

E

V

I

A

A

S

T

K

Y

L

N

D

D

H
|
H

R
|
R

I

M

A

A

M

M

S

C

M

F

A

S

V

L

A

V

G

A

L

L

G

-

A

S

K

D

A

T

D

P

V

V

S

T

I

I

D

L

D

D

V

P

S

K

P

C

V

T

A

A

T
x
K

S

T

Y

F

P

P

G

D

F

Y

F

F

S

E

T

Q

L

L

active site: K22 (= K27), S23 (= S28), D49 (= D54), N94 (= N98), P119 (≠ A123), R124 (= R128), D313 (≠ L310), E341 (= E353), H385 (= H400), R386 (= R401), K411 (≠ T427)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: K22 (= K27), S23 (= S28), R27 (= R32), T97 (= T101), Q171 (= Q175), Y200 (vs. gap), D313 (≠ L310), K340 (= K352)

1x8tA Epsps liganded with the (r)-phosphonate analog of the tetrahedral reaction intermediate (see paper)
29% identity, 94% coverage: 19:436/443 of query aligns to 14:420/427 of 1x8tA

query
sites
1x8tA

G

G

T

T

L

I

T

N

V

L

P

P

G

G

D

S

K
|
K

S
|
S

I

V

S

S

H

N

R
|
R

S

A

L

L

M

L

F

L

A

A

G

A

L

L

A

A

V

H

G

G

E

K

S

T

R

V

I

L

E

T

G

N

L

L

E

D

G

S

E

D

D
|
D

V

V

L

R

A

H

T

M

A

L

A

N

A

A

M

L

R

T

A

A

M

L

G

G

A

V

K

S

I

Y

E

T

R

L

D

S

G

A

D

D

G

R

V

T

W

-

H

R

V

C

W

E

G

I

V

I

G

G

V

N

G

G

L

P

L

L

Q

H

P

A

E

E

T

G

A

A

L

L

E

E

-

L

-

F

M

L

G

G

N
|
N

S

A

G

G

T
|
T

S

A

T

M

R

R

L

P

L

L

M

A

G

A

L

A

V

L

A

C

S

L

H

G

P

S

I

N

T

D

A

I

T

V

F

L

T

T

G

G

D

E

A
x
P

S

R

L

M

S

K

K

E

R
|
R

P

P

M

I

G

G

R

H

V

L

I

V

D

D

P

A

L

L

S

R

R

L

M

G

G

G

A

A

D

K

F

I

T

T

S

Y

S

L

P

E

G

Q

G

E

R

N

L

Y

-

P

P

P

L

L

T

R

M

L

R

Q

G

G

L

G

C

F

P

T

A

G

V

G

P

N

I

V

E

D

Y

V

T

D

L

G

P

S

V

V

A

-

S
|
S

A
x
S

Q
|
Q

V

F

K

L

S

T

A

A

V

L

L

L

L

M

A

T

G

A

L

P

N

L

T

A

P

P

-

E

-

D

G

T

I

V

T

I

R

R

V

I

I

K

E

G

P

D

V

L

L

V

T
x
S

R

K

-

P

-
x
Y

-

I

D

D

H

I

S

T

E

L

R

N

M

L

L

M

A

K

G

T

F

F

G

G

A

-

N

-

L

V

T

E

V

I

E

E

E

N

T

Q

P

H

Q

Y

G

Q

R

Q

I

F

I

V

S

V

I

K

H

G

G

G

E

Q

A

S

E

Y

F

Q

K

S

P

P

Q

G

Q

T

I

Y

T

L

V

V

P

E

G

G

D

D

P

A

S

S

A

A

A

S

F

Y

W

F

M

L

V

A

A

A

S

A

I

I

V

-

P

K

G

G

S

G

D

T

I

V

T

K

V

V

A

T

N

G

V

I

G

G

L

R

N

N

P

S

T

M

R

Q

-

G

-

D

I

I

G

R

L

F

I

A

T

D

A

V

L

L

R

E

M

K

M

M

G

G

A

A

D

T

I

I

T

C

E

W

V

G

N

D

P

D

R

Y

I

I

-

S

-

C

V

T

G

R

G

G

E

E

P

L

V

N

A

A

-

I

D

D

L

M

R

D

V

M

R

N

H

H

A

I

P

P

L
x
D

S

A

A

A

I

M

E

T

V

I

P

A

H

-

D

-

L

-

A

-

P

-

S

-

M

-

I

-

D

-

E

-

Y

-

P

-

V

-

L

-

F

-

V

T

A

A

A

L

F

F

A

A

V

K

G

G

R

T

T

T

I

T

A

L

R

R

G

N

A

I

E

Y

E
x
N

L

W

R

R

V

V

K
|
K

E
|
E

S

T

D

D

R
|
R

I

L

A

F

A

A

M

M

V

A

A

T

A

E

L

L

A

R

P

K

N

V

G

G

V

A

P

E

L

V

E

E

F

G

E

H

D

D

G

Y

L

I

A

R

I

I

T

T

G

P

T

P

G

-

P

E

I

K

A

L

G

N

G

F

A

A

P

E

V

I

A

A

S

T

K

Y

L

N

D

D

H
|
H

R
|
R

I

M

A

A

M

M

S

C

M

F

A

S

V

L

A

V

G

A

L

L

G

-

A

S

K

D

A

T

D

P

V

V

S

T

I

I

D

L

D

D

V

P

S

K

P

C

V

T

A

A

T
x
K

S

T

Y

F

P

P

G

D

F

Y

F

F

S

E

T

Q

L

L

active site: K22 (= K27), S23 (= S28), D49 (= D54), N94 (= N98), P119 (≠ A123), R124 (= R128), D313 (≠ L310), E341 (= E353), H385 (= H400), R386 (= R401), K411 (≠ T427)
binding [3r-[3a,4a,5b(r*)]]-5-(1-carboxy-1-phosphonoethoxy)-4-hydroxy-3-(phosphonooxy)-1-cyclohexene-1-carboxylic acid: K22 (= K27), S23 (= S28), R27 (= R32), T97 (= T101), S169 (= S173), S170 (≠ A174), Q171 (= Q175), S197 (≠ T199), Y200 (vs. gap), D313 (≠ L310), N336 (≠ E348), K340 (= K352), R344 (= R356), H385 (= H400), R386 (= R401)

Query Sequence

>Ga0059261_0167 FitnessBrowser__Korea:Ga0059261_0167
MSSAPPLPLAISARGPLRGTLTVPGDKSISHRSLMFAGLAVGESRIEGLLEGEDVLATAA
AMRAMGAKIERDGDGVWHVWGVGVGGLLQPETALEMGNSGTSTRLLMGLVASHPITATFT
GDASLSKRPMGRVIDPLSRMGADFTSSPGGRLPLTMRGLCPAVPIEYTLPVASAQVKSAV
LLAGLNTPGITRVIEPVLTRDHSERMLAGFGANLTVEETPQGRIISIHGEAEFKPQQITV
PGDPSSAAFWMVAASIVPGSDITVANVGLNPTRIGLITALRMMGADITEVNPRIVGGEPV
ADLRVRHAPLSAIEVPHDLAPSMIDEYPVLFVAAAFAVGRTIARGAEELRVKESDRIAAM
VAALAPNGVPLEEFEDGLAITGTGGGPIAGGAPVASKLDHRIAMSMAVAGLGAKADVSID
DVSPVATSYPGFFSTLDSLTGRT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory