SitesBLAST

Q8IJR9 GMP synthase [glutamine-hydrolyzing]; PfGMPS; Glutamine amidotransferase; Guanosine monophosphate synthetase; EC 6.3.5.2 from Plasmodium falciparum (isolate 3D7) (see 3 papers)
40% identity, 99% coverage: 6:518/518 of query aligns to 7:555/555 of Q8IJR9

query
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Q8IJR9

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Y18 (≠ V17) mutation to F: Slight increase in affinity for glutamine. No defect in glutaminase activity.
H20 (≠ Q19) mutation to A: Slight decrease in affinity for glutamine. 1.8-fold increase in affinity for ATP. Slight increase in affinity for XMP. Moderate reduction in glutaminase activity.
K24 (≠ R23) mutation to L: 50 percent decrease in glutaminase activity. 5.3-fold decrease in affinity for glutamine. 1.7-fold increase in affinity for ATP. 2.8-fold decrease in affinity for XMP.
R25 (= R24) mutation to L: No effect on glutaminase activity. 1.4-fold decrease in affinity for glutamine.
C89 (= C84) mutation to A: Loss of glutaminase activity, however, glutamine binding is not affected. In presence of exogenous ammonia, the amination of XMP to produce GMP is normal. 2.3-fold decrease in affinity for ATP and 1.8-fold decrease in affinity for XMP. 2.9-fold decrease in affinity for ATP and 1.9-fold decrease in affinity for XMP; when associated with A-113.
Q93 (= Q88) binding
C113 (≠ R108) mutation to A: 2.9-fold decrease in affinity for ATP and 1.9-fold decrease in affinity for XMP; when associated with A-89.
K160 (≠ W124) mutation to L: No effect on glutaminase activity. 1.2-fold decrease in affinity for ATP. 1.8-fold decrease in affinity for XMP.
W167 (= W133) mutation to F: Slight decrease in affinity for glutamine. Slight increase in glutaminase activity.
N169 (≠ S135) binding ; mutation to S: Slight increase in affinity for glutamine. No defect in glutaminase activity.
D172 (= D138) binding ; mutation to A: 172-fold decrease in affinity for glutamine. Severe loss of glutaminase activity.
H208 (= H174) binding
Y212 (≠ V178) mutation to W: 2.7-fold decrease in affinity for glutamine. No defect in glutaminase activity.
E213 (≠ H179) mutation to A: 40 percent decrease in glutaminase activity. 1.4-fold decrease in affinity for glutamine. 1.3-fold decrease in affinity for ATP. 1.8-fold decrease in affinity for XMP.
R336 (= R301) binding
D371 (= D333) Important for ATPPase activity; mutation to A: Impaired formation of adenyl-XMP intermediate. Slight increase in glutaminase activity.
E374 (= E336) mutation to L: 8.9-fold decrease in affinity for ammonia. Severe loss of glutaminase activity.
K376 (≠ S339) mutation to L: 20 percent decrease in glutaminase activity. 4.4-fold decrease in affinity for glutamine. 1.8-fold decrease in affinity for XMP.
K386 (= K349) mutation to L: Severe loss of ATP pyrophosphatase (ATPPase) activity. 80 percent decrease in glutaminase activity. Impaired GMP formation.
T387 (≠ S350) mutation to A: No effect on ATP pyrophosphatase (ATPPase) activity. 20 percent decrease in glutaminase activity. No effect on GMP formation.
H388 (= H351) Important for ATPPase activity; mutation to A: Moderate decrease in ATP pyrophosphatase (ATPPase) activity. Reduces 49 percent decrease in glutaminase activity. Impaired GMP formation.
H389 (= H352) Important for ATPPase activity; mutation to A: Loss of ATP pyrophosphatase (ATPPase) activity. 67 percent decrease in glutaminase activity. Impaired GMP formation.
N390 (= N353) mutation to A: No effect on ATP pyrophosphatase (ATPPase) activity. Increases glutaminase activity. Loss of GMP formation.
K411 (= K374) mutation to L: 70 percent decrease in glutaminase activity. Loss of GMP formation.
D412 (= D375) mutation to A: 30 percent decrease in glutaminase activity. 7.9-fold decrease in affinity for glutamine.
D413 (≠ E376) mutation to A: 35 percent decrease in glutaminase activity. 3.6-fold decrease in affinity for glutamine.
K415 (≠ R378) mutation to L: Increases glutaminase activity. 4.2-fold decrease in affinity for ATP.
Q476 (= Q439) binding
R539 (= R502) mutation to L: 85 percent decrease in glutaminase activity.
K547 (= K510) binding ; mutation to L: 85 percent decrease in glutaminase activity.
I552 (= I515) binding
E553 (= E516) binding ; mutation to L: 85 percent decrease in glutaminase activity.
E555 (= E518) mutation to L: 20 percent decrease in glutaminase activity. No effect on GMP formation.

2ywcA Crystal structure of gmp synthetase from thermus thermophilus in complex with xmp
47% identity, 99% coverage: 8:518/518 of query aligns to 2:475/475 of 2ywcA

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active site: G51 (≠ S57), R53 (≠ A59), C78 (= C84), Y79 (= Y85), H164 (= H174), E166 (= E176), D221 (= D232), K343 (= K374)
binding xanthosine-5'-monophosphate: R288 (= R301), P366 (= P397), G367 (= G398), P368 (= P399), Q408 (= Q439), K467 (= K510), T471 (= T514), I472 (= I515), E473 (= E516)

4wioA Crystal structure of the c89a gmp synthetase inactive mutant from plasmodium falciparum in complex with glutamine (see paper)
38% identity, 99% coverage: 6:518/518 of query aligns to 1:525/525 of 4wioA

query
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4wioA

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G

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x
A

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|
Y

G

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|
Q

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K

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A

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T

I

I

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active site: G52 (≠ S57), A83 (≠ C84), Y84 (= Y85), H197 (= H174), E199 (= E176), D255 (= D232), K393 (= K374)
binding glutamine: Q87 (= Q88), N158 (≠ S135), H159 (= H136), N160 (≠ G137), D161 (= D138), H197 (= H174)

3uowA Crystal structure of pf10_0123, a gmp synthetase from plasmodium falciparum
37% identity, 99% coverage: 6:518/518 of query aligns to 2:517/517 of 3uowA

query
sites
3uowA

E

D

S

K

I

I

L

L

I

V

V

L

D

N

F

F

G

G

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S

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Q

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M

M

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N

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K

G

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V

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L

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x
G

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|
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|
Y

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C

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|
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|
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D

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V

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A

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N

R

R

H

H

P

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F

F

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|
P

G
|
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P
|
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G

G

L

L

A

A

I

I

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R

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P

I

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G

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E

V

I

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K

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C

L

D

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I

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L

L

R

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D

D

A

D

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F

L

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D

I

L

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K

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A

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G

G

L

L

Y

Y

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N

A

Q

I

I

W

S

Q
|
Q

A

A

F

F

A

A

V

V

L

L

P

S

V

S

K

K

T

S

V

V

G

-

V

-

M

-

G

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D

-

G

-

R

-

T

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Y

Y

D

D

S

Y

V

V

C

C

G

V

L

L

R

R

A

A

V

V

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K

S

T

T

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D

S

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F

M

M

T

T

A

A

D

N

V

W

Y

Y

P

Q

F

I

D

P

A

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S

D

F

I

L

L

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D

R

K

V

I

A

T

T

T

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R

I

I

V

L

N

S

E

E

V

V

K

K

G

G

V

V

N

N

R

R

V

I

V

L

Y

Y

D

D

Y

V

T

S

S

S

K
|
K

P

P

G

A

T
|
T

I
|
I

E
|
E

W

F

E

E

active site: G53 (≠ S57), C84 (= C84), Y85 (= Y85), H198 (= H174), E200 (= E176), D255 (= D232), K381 (= K374)
binding xanthosine-5'-monophosphate: R325 (= R301), P404 (= P397), G405 (= G398), P406 (= P399), Q446 (= Q439), K509 (= K510), T513 (= T514), I514 (= I515), E515 (= E516)

3uowB Crystal structure of pf10_0123, a gmp synthetase from plasmodium falciparum
38% identity, 99% coverage: 8:518/518 of query aligns to 2:477/477 of 3uowB

query
sites
3uowB

I

I

L

L

I

V

V

L

D

N

F

F

G

G

S

S

Q

Q

V

Y

T

F

Q

H

L

L

I

I

A

V

R

K

R

R

V

L

R

N

E

N

A

I

G

K

V

I

Y

F

S

S

E

E

I

T

A

K

P

D

F

Y

N

K

A

D

A

I

A

K

E

D

A

-

F

-

E

-

R

-

M

M

K

N

P

I

G

K

G

G

V

V

I

I

L

L

S

S

G

G

S
x
G

P

P

A

Y

S

S

V

-

L

-

E

-

D

-

G

-

S

-

P

-

R

E

V

V

P

F

Q

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A

Y

I

F

F

L

E

E

S

K

G

K

L

I

P

P

V

I

L

F

G

G

I

I

C
|
C

Y
|
Y

G

G

Q

M

Q

Q

V

E

M

I

M

A

Q

V

Q

Q

L

M

G

N

G

Y

-

G

-

C

-

T

-

D

-

V

N

N

V

I

Q

N

L

I

G

N

D

N

S

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G

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Y

F

C

G

A

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M

A

D

F

L

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I

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A

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D

K

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L

-

M

S

N

C

C

V

C

L

L

F

F

D

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G

N

L

I

W

K

A

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D

G

I

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T

T

T

H

-

Q

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V

V

W

W

M

M

S

N

H

H

G

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D

D

K

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D

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H
|
H

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|
E

V

V

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L

D

D

G

G

A

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K

L

L

M

L

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A

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N

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A

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F

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K

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L

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I

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K

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K

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H

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G

H

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Y

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V

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C

A

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M

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S

G

G

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|
D

S

S

A

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V

V

A

A

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A

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H

H

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A

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G

G

L

L

M

L

R

R

S

K

G

N

E

E

A

A

D

E

Q

N

V

V

V

Y

S

T

L

F

F

L

R

K

G

S

H

T

Y

F

-

P

N

D

I

M

P

N

L

I

V

T

H

K

V

I

N

D

A

A

E

S

T

E

L

N

F

F

L

L

N

S

G

N

L

L

A

Q

G

G

V

V

T

T

D

D

P

P

E

E

A

Q

K

K

R
|
R

K

K

F

I

I

I

G

G

K

K

T

L

F

F

I

I

D

E

V

E

F

F

E

E

E

K

E

A

A

V

K

N

I

I

G

D

-

D

-

I

G

N

A

K

E

T

F

F

L

L

A

L

Q

Q

G

G

T

T

L

L

Y

Y

P

P

D

D

V

I

I

I

E

E

S

S

V

K

S

K

F

F

T

-

G

-

P

-

S

-

V

-

T

-

I

-

K

-

S

-

H

-

N

-

V

-

G

-

L

-

P

-

E

-

R

-

M

-

N

-

M

-

K

K

L

L

V

F

E

E

P

P

L

F

R

K

E

Y

L

L

F

F

K
|
K

D

D

E

D

V

V

R

K

A

T

L

L

G

S

R

R

E

E

L

L

G

N

L

L

P

P

D

E

V

E

F

I

V

T

G

N

R

R

H

H

P

P

F

F

P
|
P

G
|
G

P
|
P

G

G

L

L

A

A

I

I

R

R

I

V

P

I

G

G

E

E

V

I

T

N

K

K

E

H

R

K

C

L

D

N

I

I

L

L

R

R

K

E

A

V

D

D

A

D

V

I

Y

F

L

I

E

N

E

D

I

L

R

K

N

Q

A

Y

G

G

L

L

Y

Y

D

N

A

Q

I

I

W

S

Q
|
Q

A

A

F

F

A

A

V

V

L

L

P

S

V

S

K

K

T

S

V

V

G

G

V

-

M

-

G

-

D

-

G

-

R

-

T

-

Y

Y

D

D

S

Y

V

V

C

C

G

V

L

L

R

R

A

A

V

V

T

K

S

T

T

S

D

S

G

F

M

M

T

T

A

A

D

N

V

W

Y

Y

P

Q

F

I

D

P

A

Y

S

D

F

I

L

L

T

D

R

K

V

I

A

T

T

T

R

R

I

I

V

L

N

S

E

E

V

V

K

K

G

G

V

V

N

N

R

R

V

I

V

L

Y

Y

D

D

Y

V

T

S

S

S

K
|
K

P

P

G

A

T
|
T

I
|
I

E
|
E

W

F

E

E

active site: G47 (≠ S57), C67 (= C84), Y68 (= Y85), H162 (= H174), E164 (= E176), D218 (= D232), K340 (= K374)
binding xanthosine-5'-monophosphate: R288 (= R301), P363 (= P397), G364 (= G398), P365 (= P399), Q405 (= Q439), K469 (= K510), T473 (= T514), I474 (= I515), E475 (= E516)

2vxoB Human gmp synthetase in complex with xmp (see paper)
38% identity, 98% coverage: 7:515/518 of query aligns to 5:540/658 of 2vxoB

query
sites
2vxoB

S

A

I

V

L

V

I

I

V

L

D

D

F

A

G

G

S

A

Q

Q

V

Y

T

G

Q

K

L

V

I

I

A

D

R

R

V

V

R

R

E

E

A

L

G

F

V

V

Y

Q

S

S

E

E

I

I

A

F

P

P

F

L

N

E

A

T

A

P

A

A

E

F

A

A

F

I

E

K

R

E

M

Q

K

G

P

F

G

R

G

A

V

I

I

I

L

I

S

S

G

G

S
x
G

P

P

A

N

S

S

V

V

L

Y

E

A

D

E

G

D

S

A

P

P

R

W

V

F

P

D

Q

P

A

A

I

I

F

F

E

T

S

I

G

G

L

K

P

P

V

V

L

L

G

G

I

I

C
|
C

Y
|
Y

G

G

Q

M

Q

Q

V

M

M

M

M

N

Q

K

Q

V

L

F

G

G

N

T

V

V

Q

H

L

K

G

K

D

S

S

D

G

G

E

V

F

F

G

N

R

-

A

-

F

-

I

I

E

S

I

V

A

D

D

N

S

T

C

C

V

S

L

L

F

F

D

R

G

G

L

L

W

Q

A

K

E

E

G

-

E

-

T

-

H

E

Q

V

V

V

W

L

M

L

S

T

H

H

G

G

D

D

K

S

V

V

T

D

S

K

L

V

A

A

P

D

G

G

F

F

R

K

P

-

V

V

A

V

A

A

S

R

A

S

G

G

A

N

P

I

F

V

A

A

V

G

I

I

A

A

D

N

D

E

T

S

R

K

Y

L

Y

Y

A

G

M

A

Q

Q

F

F

H
|
H

P

P

E
|
E

V

V

V

G

H

L

T

T

P

E

D

N

G

G

A

K

K

V

L

I

L

L

A

K

N

N

F

F

V

L

R

Y

H

D

V

I

C

A

G

G

L

C

K

S

G

G

D

T

W

F

T

T

M

V

A

Q

E

N

F

R

R

E

Q

L

T

E

K

C

I

I

E

R

E

E

I

I

R

K

K

E

Q

R

V

V

G

G

T

T

G

S

K

K

V

V

I

L

C

V

G

L

L

L

S

S

G

G

V

V

D
|
D

S

S

A

T

V

V

A

C

A

T

V

A

L

L

I

L

H

N

E

R

A

A

I

L

G

N

-

Q

E

E

Q

Q

L

V

T

I

C

A

V

V

F

H

V

I

D

D

H

N

G

G

L

F

M

M

R

R

S

K

G

R

E

E

A

S

D

Q

Q

S

V

V

V

E

S

E

L

A

F

L

R

K

G

-

H

K

Y

L

N

G

I

I

P

Q

L

V

V

K

H

V

V

I

N

N

A

A

E

A

T

H

L

S

F

F

L

Y

N

N

G

G

-

T

-

L

-

P

-

R

-

I

-

S

-

K

-

T

L

L

A

N

G

M

V

T

T

T

D

S

P

P

E

E

A

E

K

K

R
|
R

K

K

F

I

I

I

G

G

K

D

T

T

F

F

I

V

D

K

V

I

F

A

E

N

E

E

-

V

-

I

-

G

E

E

A

M

K

N

K

L

I

K

G

P

G

E

A

E

E

V

F

F

L

L

A

A

Q

Q

G

G

T

T

L

L

Y

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P

P

D

D

V

L

I

I

E

E

S

S

V

A

S

S

F

L

T

V

G

A

-

S

G
|
G

P
x
K

S

A

V

E

T

L

I

I

K

K

S

T

H

H

N

N

V

D

G

T

G

E

L

L

P

I

E

R

R

K

M

L

N

R

M

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-

E

-

G

K

K

L

V

V

I

E

E

P

P

L

L

R

K

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D

L

F

F

H

K
|
K

D

D

E

E

V

V

R

R

A

I

L

L

G

G

R

R

E

E

L

L

G

G

L

L

P

P

D

E

V

E

F

L

V

V

G

S

R

R

H

H

P

P

F

F

P
|
P

G
|
G

P
|
P

G

G

L

L

A

A

I

I

R

R

I

V

-

I

-

C

-

A

-

E

-

P

-

Y

-

I

-

C

-

K

-

D

-

F

-

P

-

E

P

T

G

N

E

N

V

I

T

L

K

K

E

I

R

V

C

A

D

D

-

F

-

S

-

A

-

S

-

V

-

K

-

P

-

H

-

T

I

L

L

L

R

Q

K

R

A

V

D

K

A

A

V

C

Y

T

L

T

E

E

I

D

R

Q

N

E

A

K

G

L

L

M

-

Q

Y

I

D

T

A

S

I

L

W

H

Q

S

A

L

F

N

A

A

V

F

L

L

P

P

V

I

K

K

T

T

V

V

G

G

V

V

M

Q

G

G

D

D

G

C

R
|
R

T

S

Y

Y

D

S

S

Y

V

V

C

C

G

G

L

I

R

S

A

S

V

K

T

D

S

E

T

P

D

D

G

W

M

E

T

S

A

-

D

-

V

-

Y

-

P

-

F

-

D

-

A

L

S

I

F

F

L

L

T

A

R

R

V

L

A

I

T

P

R

R

I

M

V

C

N

H

E

-

V

-

K

-

G

N

V

V

N

N

R

R

V

V

Y

Y

D

I

Y

F

-

G

-

P

T

V

S

K

K

E

P

P

G

T

T

D

I

V

active site: G55 (≠ S57), C82 (= C84), Y83 (= Y85), H165 (= H174), E167 (= E176), D223 (= D232), K381 (= K374)
binding xanthosine-5'-monophosphate: R302 (= R301), G348 (= G343), K349 (≠ P344), P404 (= P397), G405 (= G398), P406 (= P399), R489 (= R457)

Sites not aligning to the query:

binding xanthosine-5'-monophosphate: 575, 610, 650, 654, 655, 656

P49915 GMP synthase [glutamine-hydrolyzing]; GMP synthetase; Glutamine amidotransferase; EC 6.3.5.2 from Homo sapiens (Human) (see paper)
37% identity, 98% coverage: 7:515/518 of query aligns to 27:575/693 of P49915

query
sites
P49915

S

A

I

V

L

V

I

I

V

L

D

D

F

A

G

G

S

A

Q

Q

V

Y

T

G

Q

K

L

V

I

I

A

D

R

R

V

V

R

R

E

E

A

L

G

F

V

V

Y

Q

S

S

E

E

I

I

A

F

P

P

F

L

N

E

A

T

A

P

A

A

E

F

A

A

F

I

E

K

R

E

M

Q

K

G

P

F

G

R

G

A

V

I

I

I

L

I

S

S

G

G

S

G

P

P

A

N

S

S

V

V

L

Y

E

A

D

E

G

D

S

A

P

P

R

W

V

F

P

D

Q

P

A

A

I

I

F

F

E

T

S

I

G

G

L

K

P

P

V

V

L

L

G

G

I

I

C
|
C

Y

Y

G

G

Q

M

Q

Q

V

M

M

M

M

N

Q

K

Q

V

L

F

G

G

N

T

V

V

Q

H

L

K

G

K

D

S

S

V

G

R

E

E

F

D

G

G

R

V

A

F

F

N

I

I

E

S

I

V

A

D

D

N

S

T

C

C

V

S

L

L

F

F

D

R

G

G

L

L

W

Q

A

K

E

E

G

-

E

-

T

-

H

E

Q

V

V

V

W

L

M

L

S

T

H

H

G

G

D

D

K

S

V

V

T

D

S

K

L

V

A

A

P

D

G

G

F

F

R

K

P

-

V

V

A

V

A

A

S

R

A

S

G

G

A

N

P

I

F

V

A

A

V

G

I

I

A

A

D

N

D

E

T

S

R

K

Y

L

Y

Y

A

G

M

A

Q

Q

F

F

H
|
H

P

P

E
|
E

V

V

V

G

H

L

T

T

P

E

D

N

G

G

A

K

K

V

L

I

L

L

A

K

N

N

F

F

V

L

R

Y

H

D

V

I

C

A

G

G

L

C

K

S

G

G

D

T

W

F

T

T

M

V

A

Q

E

N

F

R

R

E

Q

L

T

E

K

C

I

I

E

R

E

E

I

I

R

K

K

E

Q

R

V

V

G

G

T

T

G

S

K

K

V

V

I

L

C

V

G

L

L

L

S

S

G

G

V

V

D

D

S

S

A

T

V

V

A

C

A

T

V

A

L

L

I

L

H

N

E

R

A

A

I

L

G

N

-

Q

E

E

Q

Q

L

V

T

I

C

A

V

V

F

H

V

I

D

D

H

N

G

G

L

F

M

M

R

R

S

K

G

R

E

E

A

S

D

Q

Q

S

V

V

V

E

S

E

L

A

F

L

R

K

G

-

H

K

Y

L

N

G

I

I

P

Q

L

V

V

K

H

V

V

I

N

N

A

A

E

A

T

H

L

S

F

F

L

Y

N

N

G

G

-

T

-

L

-

P

-

I

-

S

-

D

-

E

-

D

-

R

-

T

-

P

-

R

-

K

-

R

-

I

-

S

-

K

-

T

L

L

A

N

G

M

V

T

T

T

D

S

P

P

E

E

A

E

K

K

R
|
R

K

K

F

I

I

I

G

G

K

D

T

T

F

F

I

V

D

K

V

I

F

A

E

N

E

E

-

V

-

I

-

G

E

E

A

M

K

N

K

L

I

K

G

P

G

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A

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F

F

L

L

A

A

Q

Q

G

G

T

T

L

L

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P

P

D

D

V

L

I

I

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A

-

S

G

G

P

K

S

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L

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I

K

K

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H

N

N

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D

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L

L

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R

K

M

L

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M

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K

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I

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P

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L

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F

H

K

K

D

D

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V

V

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A

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L

L

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G

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L

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G

L

L

P

P

D

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V

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F

L

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V

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R

R

H

H

P

P

F

F

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P

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G

P

P

G

G

L

L

A

A

I

I

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R

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V

-

I

-

C

-

A

-

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-

P

-

Y

-

I

-

C

-

K

-

D

-

F

-

P

-

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P

T

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N

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N

V

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T

L

K

K

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A

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D

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F

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A

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K

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H

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I

L

L

L

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K

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D

K

A

A

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C

Y

T

L

T

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D

R

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N

E

A

K

G

L

L

M

-

Q

Y

I

D

T

A

S

I

L

W

H

Q

S

A

L

F

N

A

A

V

F

L

L

P

P

V

I

K

K

T

T

V

V

G

G

V

V

M

Q

G

G

D
|
D

G

C

R

R

T

S

Y

Y

D

S

S

Y

V

V

C

C

G

G

L

I

R

S

A

S

V

K

T

D

S

E

T

P

D

D

G

W

M

E

T

S

A

-

D

-

V

-

Y

-

P

-

F

-

D

-

A

L

S

I

F

F

L

L

T

A

R

R

V

L

A

I

T

P

R

R

I

M

V

C

N

H

E

-

V

-

K

-

G

N

V

V

N

N

R

R

V

V

Y

Y

D

I

Y

F

-

G

-

P

T

V

S

K

K

E

P

P

G

T

T

D

I

V

C104 (= C84) active site, For GATase activity
H190 (= H174) active site, For GATase activity
E192 (= E176) active site, For GATase activity
R337 (= R301) binding
D522 (= D455) binding

Sites not aligning to the query:

610 binding
685 binding
691 binding

6jp9A Crsytal structure of a xmp complexed atppase subunit of m. Jannaschii gmp synthetase (see paper)
53% identity, 61% coverage: 205:518/518 of query aligns to 6:298/298 of 6jp9A

query
sites
6jp9A

E

K

F

F

R

I

Q

D

T

E

K

A

I

V

E

E

I

I

R

K

K

Q

Q

Q

V

I

G

S

T

D

G

R

K

K

V

A

I

I

C

I

G

A

L

L

S

S

G

G

V

V

D
|
D

S

S

A

S

V

V

A

A

V

V

L

L

I

T

H

H

E

K

A

A

I

I

G

G

E

D

Q

K

L

L

T

T

C

A

V

V

F

F

V

V

D

D

H

T

G

G

L

L

M

M

R

R

S

K

G

G

E

E

A

R

D

E

Q

E

V

V

V

E

S

K

L

T

F

F

R

R

G

D

H

K

Y

L

N

G

I

L

P

N

L

L

V

I

H

V

V

V

N

D

A

A

E

K

T

D

L

R

F

F

L

L

N

N

G

A

L

L

A

K

G

G

V

V

T

T

D

D

P

P

E

E

A

E

K

K

R
|
R

K

K

F

I

I

I

G

G

K

K

T

L

F

F

I

I

D

D

V

V

F

F

E

E

E

I

A

A

K

E

K

D

I

I

G

-

G

K

A

A

E

E

F

V

L

L

A

V

Q

Q

G

G

T

T

L

I

Y

A

P

P

D

D

V

-

I

-

E

-

S

-

V

-

S

-

F

-

T

-

G

-

P

-

S

-

V

-

T

-

I

-

K

-

S

-

H

-

H

H

N

N

V

V

G

-

G

A

L

L

P

P

E

H

R

G

M

M

N

V

M

L

K

E

L

V

V

V

E

E

P

P

L

L

R

R

E

E

L

L

F

Y

K
|
K

D

D

E

E

V

V

R

R

A

L

L

L

G

A

R

K

E

E

L

L

G

G

L

L

P

P

D

D

V

S

F

I

V

V

G

Y

R

R

H

Q

P

P

F

F

P
|
P

G
|
G

P
|
P

G

G

L

L

A

A

I

V

R

R

I

V

P

L

G

G

E

E

V

V

T

T

K

E

E

E

R

K

C

L

D

N

I

I

L

C

R

R

K

E

A

A

D

N

A

A

V

I

Y

V

L

E

E

E

I

V

R

K

N

K

A

A

G

N

L

L

Y

D

D

K

A

D

I

L

W

W

Q
|
Q

A

Y

F

F

A

A

V

V

L

V

L

L

P

D

V

C

K

K

T

A

V

T

G

G

V

V

M

K

G

G

D

D

G

-

R

R

T

E

Y

Y

D

N

S

W

V

I

C

V

G

A

L

L

R

R

A

M

V

V

T

K

S

S

T

L

D

D

G

A

M

M

T

T

A

A

D

H

V

V

-

P

-

E

Y

I

P

P

F

F

D

D

A

-

S

-

F

L

L

L

T

K

R

R

V

I

A

S

T

K

R

R

I

I

V

T

N

S

E

E

V

I

K

P

G

N

V

V

N

A

R

R

V

V

Y

F

D

D

Y

I

T

T

S

D

K
|
K

P

P

G

A

T
|
T

I
|
I

E
|
E

W

F

E

E

active site: D33 (= D232), K155 (= K374)
binding xanthosine-5'-monophosphate: R102 (= R301), P178 (= P397), G179 (= G398), P180 (= P399), Q220 (= Q439), K290 (= K510), T294 (= T514), I295 (= I515), E296 (= E516)

7yc6A Crystal structure of d110p mutant of gatase subunit of methanocaldococcus jannaschii gmp synthetase
34% identity, 37% coverage: 8:198/518 of query aligns to 2:181/183 of 7yc6A

query
sites
7yc6A

I

I

L

V

I

I

V

L

D

D

F

N

G

G

S

G

Q

Q

V

Y

T

V

Q

H

L

R

I

I

A

H

R

R

R

S

V

L

R

K

E

Y

A

I

G

G

V

V

Y

S

S

S

E

K

I

I

A

V

P

P

F

N

N

T

A

T

A

P

A

L

E

E

A

E

F

I

E

E

R

S

M

N

K

K

P

E

-

V

G

K

G

G

V

I

I

I

L

L

S

S

G

G

S

G

P

P

A

D

S

-

V

-

L

I

E

E

D

K

G

A

S

K

P

N

R

C

V

I

P

D

Q

I

A

A

I

L

F

-

E

N

S

A

G

K

L

L

P

P

V

I

L

L

G

G

I

I

C
|
C

Y

L

G

G

Q

H

Q

Q

V

L

M

I

M

A

Q

L

Q

A

L

Y

G

G

N

E

V

V

Q

-

L

-

G

-

D

-

S

-

G

G

E

R

F

A

G

A

R

L

A

T

F

K

I

V

E

Y

I

V

A
x
D

D

K

S

E

C

N

V

P

L

L

F

F

D

K

G

N

L

V

W

P

A

R

E

E

G

-

E

-

T

-

H

F

Q

N

V

A

W

W

M

A

S

S

H

H

G

K

D

D

K
x
E

V

V

T

K

S

K

L

V

A

P

P

E

G

G

F

F

R

E

P

I

V

L

A

A

A
x
H

S

S

A

D

G

I

A
x
C

P

Q

F

V

A

E

V

A

I

M

A

K

D

H

D

K

T

T

R

K

R

P

Y

I

Y

Y

A

G

M

V

Q

Q

F

F

H
|
H

P

P

E

E

V

V

V

A

H

H

T

T

P

E

D

Y

G

G

A

N

K

E

L

I

L

L

A

K

N

N

F

F

V

C

R

K

H

-

V

V

C

C

G

G

L

Y

K

K

binding zinc ion: C76 (= C84), D101 (≠ A114), E123 (≠ K139), E123 (≠ K139), H136 (≠ A152), C140 (≠ A156), C140 (≠ A156), H158 (= H174)

P00900 Anthranilate synthase component 2; AS; ASII; Anthranilate synthase, GATase component; Anthranilate synthase, glutamine amidotransferase component; EC 4.1.3.27 from Serratia marcescens (see 3 papers)
30% identity, 35% coverage: 8:187/518 of query aligns to 4:183/193 of P00900

query
sites
P00900

I

I

L

L

I

L

V

L

D

D

F

N

G

V

S

D

Q

S

V

F

T

T

Q

Y

L

N

I

L

A

V

R

D

R

Q

V

L

R

R

E

A

A

S

G

G

-

H

-

Q

-

V

V

I

Y

Y

S

R

E

N

I

Q

A

I

P

G

F

A

N

E

A

V

A

I

E

E

A

R

F

L

E

Q

R

H

M

M

K

E

P

Q

G

P

G

V

V

L

I

M

L

L

S

S

G

P

S

G

P

P

A

G

S

T

V

P

L

S

E

E

D

A

G

G

S

C

-

M

P

P

R

E

V

L

P

L

Q

Q

A

R

I

L

F

-

E

R

S

G

G

Q

L

L

P

P

V

I

L

I

G

G

I

I

C
|
C

Y

L

G

G

Q

H

Q

Q

V

A

M

I

M

V

Q

E

Q

A

L

Y

G

G

N

-

V

-

Q

Q

L

V

G

G

D

Q

S

A

G

G

E

E

F

I

-

L

-

H

G

G

R

K

A

A

F

S

I

A

E

I

I

A

A

H

D

D

S

G

C

E

V

G

L

M

F

F

D

A

G

G

L

M

W

-

A

-

E

-

G

A

E

N

T

P

H

L

Q

P

V

V

W

A

M

R

S

Y

H

H

G

-

D

S

K

L

V

V

T

G

S

S

L

N

A

I

P

P

G

A

F

D

R

L

P

T

V

V

A

N

A

A

S

R

A

S

G

G

A

E

P

M

F

V

A

M

V

A

I

V

A

R

D

D

T

R

R

R

Y

V

Y

C

A

G

M

F

Q

Q

F

F

H

H

P

P

E

E

V

S

V

I

H

L

T

T

P

T

D

H

G

G

A

A

K

R

L

L

C84 (= C84) active site, Nucleophile; for GATase activity

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1i7qB Anthranilate synthase from s. Marcescens (see paper)
30% identity, 35% coverage: 8:187/518 of query aligns to 3:182/192 of 1i7qB

query
sites
1i7qB

I

I

L

L

I

L

V

L

D

D

F

N

G

V

S

D

Q

S

V

F

T

T

Q

Y

L

N

I

L

A

V

R

D

R

Q

V

L

R

R

E

A

A

S

G

G

-

H

-

Q

-

V

V

I

Y

Y

S

R

E

N

I

Q

A

I

P

G

F

A

N

E

A

V

A

I

E

E

A

R

F

L

E

Q

R

H

M

M

K

E

P

Q

G

P

G

V

V

L

I

M

L

L

S

S

G
x
P

S
x
G

P

P

A
x
G

S

T

V

P

L

S

E

E

D

A

G

G

S

C

-

M

P

P

R

E

V

L

P

L

Q

Q

A

R

I

L

F

-

E

R

S

G

G

Q

L

L

P

P

V

I

L

I

G

G

I

I

C
|
C

Y
x
L

G

G

Q

H

Q
|
Q

V

A

M

I

M

V

Q

E

Q

A

L

Y

G

G

N

-

V

-

Q

Q

L

V

G

G

D

Q

S

A

G

G

E

E

F

I

-

L

-

H

G

G

R

K

A

A

F

S

I

A

E

I

I

A

A

H

D

D

S

G

C

E

V

G

L

M

F

F

D

A

G

G

L

M

W

-

A

-

E

-

G

A

E

N

T

P

H

L

Q

P

V

V

W

A

M

R

S

Y

H
|
H

G

-

D
x
S

K
x
L

V

V

T

G

S

S

L

N

A

I

P

P

G

A

F

D

R

L

P

T

V

V

A

N

A

A

S

R

A

F

G

G

A

E

P

M

F

V

A

M

V

A

I

V

A

R

D

D

T

R

R

R

Y

V

Y

C

A

G

M

F

Q

Q

F

F

H
|
H

P

P

E
|
E

V

S

V

I

H

L

T

T

P

T

D

H

G

G

A

A

K

R

L

L

active site: G58 (≠ A59), C83 (= C84), L84 (≠ Y85), H169 (= H174), E171 (= E176)
binding glutamic acid: P55 (≠ G56), G56 (≠ S57), G58 (≠ A59), C83 (= C84), L84 (≠ Y85), Q87 (= Q88), H132 (= H136), S133 (≠ D138), L134 (≠ K139)

P07259 Multifunctional protein URA2; Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase; CPSase-ATCase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
25% identity, 37% coverage: 8:198/518 of query aligns to 229:411/2214 of P07259

query
sites
P07259

I

I

L

L

I

A

V

I

D

D

F

V

G

G

S

M

Q

K

V

Y

T

N

Q

Q

L

I

I

-

A

-

R

R

R

C

V

F

R

I

E

K

A

R

G

G

V

V

Y

E

S

L

E

K

I

V

A

V

P

P

F

W

N

N

A

Y

A

D

A

-

E

-

A

-

F

F

E

T

R

K

M

E

K

D

P

Y

G

D

G

G

V

L

I

F

L

I

S

S

G

N

S

G

P

P

A

G

-

D

-

P

S

S

V

V

L

L

E

D

D

D

G

L

S

S

P

Q

R

R

V

L

P

S

Q

N

A

V

I

L

F

E

E

A

S

K

G

K

L

T

P

P

V

V

L

F

G

G

I

I

C

C

Y

L

G

G

Q

H

Q

Q

V

L

M

I

M

A

Q

R

Q

A

L

A

G

G

G

A

N

S

-

T

-

L

-

K

V

L

Q

K

L

F

G

G

D

N

S

R

G

G

E

H

F

N

G

I

R

P

A

C

F

T

I

S

E

T

I

I

A

S

D

G

S

R

C

C

V

-

L

-

F

-

D

-

G

-

L

-

W

Y

A

I

E

T

G

S

E

Q

T

N

H

H

Q

G

V

F

W

A

M

V

S

D

H

-

G

-

D

-

K

-

V

V

T

D

S

T

L

L

A

T

P

S

G

G

F

W

R

K

P

P

V

L

A

F

A

V

S

N

A

A

G

N

A

-

P

-

F

-

A

-

V

-

I

-

A

-

D

D

T

S

R

N

R

E

-

G

-

I

-

Y

-

H

-

S

-

E

-

L

-

P

Y

Y

Y

F

A

S

M

V

Q

Q

F

F

H

H

P

P

E

E

V

S

V

T

H

P

T

G

P

P

D

R

G

D

A

T

K

E

L

F

L

L

A

F

N

D

-

V

F

F

V

I

R

Q

H

A

V

V

C

K

G

E

L

F

K

K

Sites not aligning to the query:

1857 modified: Phosphoserine; by PKA

8hx8A Crystal structure of 4-amino-4-deoxychorismate synthase from streptomyces venezuelae co-crystallized with chorismate (see paper)
31% identity, 26% coverage: 56:190/518 of query aligns to 58:187/673 of 8hx8A

query
sites
8hx8A

G

G

S

S

P

P

A

D

S

R

V

E

L

R

E

D

D

F

G

G

S

I

P

S

R

R

V

-

P

-

Q

R

A

A

I

I

F

T

E

D

S

S

G

G

L

L

P

P

V

V

L

L

G

G

I

V

C

C

Y

L

G

G

Q

H

Q

Q

V

G

M

I

M

A

Q

Q

Q

L

L

F

G

G

N

T

V

V

Q

G

L

L

G

A

D

P

S

E

G

P

E

M

F

H

G

G

R

R

A

V

F

S

I

E

E

V

I

R

A

H

D

T

S

G

C

E

V

D

L

V

F

F

D

R

G

G

L

L

W

P

A

S

E

-

G

-

E

-

T

P

H

F

Q

T

V

A

W

V

M

R

S

Y

H

H

G

S

D

L

K

A

V

A

T

T

S

D

L

L

A

P

P

D

G

E

F

L

R

E

P

P

V

L

A

A

A

W

S

S

A

D

G

D

A

G

P

V

F

V

A

M

V

G

I

L

A

R

D

H

D

R

T

E

R

K

R

P

Y

L

Y

W

A

G

M

V

Q

Q

F

F

H

H

P

P

E

E

V

S

V
x
I

H

G

T
x
S

P

D

D

F

G

G

A

R

K

E

L

I

L

M

A

A

N

N

F

F

binding magnesium ion: I175 (≠ V178), S177 (≠ T180)

Sites not aligning to the query:

binding magnesium ion: 521, 655, 658
binding tryptophan: 231, 232, 233, 241, 243, 458, 459, 460, 614

Q09794 Multifunctional protein ura1; Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase; CPSase-ATCase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
24% identity, 43% coverage: 8:231/518 of query aligns to 265:485/2244 of Q09794

query
sites
Q09794

I

I

L

L

I

V

V

I

D

D

F

V

G

G

S

M

Q

K

V

Y

T

N

Q

Q

L

I

I

-

A

-

R

R

R

C

V

F

R

L

E

N

A

R

G

G

V

V

Y

E

S

L

E

L

I

V

A

V

P

P

F

W

N

D

A

Y

A

D

A

-

E

-

A

-

F

F

E

T

R

K

M

E

K

T

P

Y

G

D

G

G

V

L

I

F

L

I

S

S

G

N

S

G

P

P

A

G

-

D

-

P

S

S

V

L

L

M

E

D

D

L

G

V

S

V

P

D

R

R

V

V

P

K

Q

R

A

V

I

L

F

E

E

S

S

K

G

T

L

V

P

P

V

V

L

F

G

G

I

I

C

C

Y

F

G

G

Q

H

Q

Q

V

I

M

M

M

A

Q

R

Q

A

L

A

G

G

G

A

N

S

V

T

Q

T

L

-

G

-

D

-

S

K

G

M

E

K

F

F

G

G

R

N

A

R

F

G

I

H

E

N

I

I

A

P

D

C

S

T

C

C

V

M

L

I

F

-

D

-

G

-

L

-

W

-

A

-

E

S

G

G

E

R

T

C

H

Y

Q

I

V

T

W

S

M

Q

S

N

H

H

G

G

D

Y

K

A

V

V

-

D

-

A

T

S

S

S

L

L

A

S

P

N

G

G

F

W

R

K

P

E

V

L

A

F

A

V

S

N

A

A

G

N

-

D

A

G

P

S

F

N

A

E

V

G

I

I

A

Y

D

N

D

T

T

E

R

Y

R

P

Y

F

A

S

M

V

Q

Q

F

F

H

H

P

P

E

E

V

S

V

T

H

P

T

G

P

P

D

R

G

D

A

T

K

E

L

F

L

L

A

F

N

D

F

V

V

F

R

I

H

D

V

V

C

V

G

K

L

R

K

S

G

A

D

D

W

A

-

K

T

S

M

L

A

Q

E

P

F

F

R

K

Q

L

T

P

-

G

K

T

I

I

E

E

I

N

R

R

K

S

Q

R

-

H

-

P

-

L

V

V

G

D

T

A

G

K

K

R

V

V

I

L

C

I

G

L

L

G

S

S

G

G

V

L

Sites not aligning to the query:

1119 modified: Phosphoserine
1881 modified: Phosphoserine
1885 modified: Phosphoserine

P00903 Aminodeoxychorismate synthase component 2; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 2; Aminodeoxychorismate synthase, glutamine amidotransferase component; EC 2.6.1.85 from Escherichia coli (strain K12) (see paper)
27% identity, 36% coverage: 8:192/518 of query aligns to 2:186/187 of P00903

query
sites
P00903

I

I

L

L

I

L

V

I

D

D

F

N

G

Y

S

D

Q

S

V

F

T

T

Q

W

L

N

I

L

A

Y

R

Q

R

Y

V

F

R

C

E

E

A

L

G

G

V

A

Y

D

S

V

E

L

I

V

A

K

P

R

F

N

N

D

A

A

A

L

A

T

E

L

A

A

-

D

F

I

E

D

R

A

M

L

K

K

P

P

G

Q

G

K

V

I

I

V

L

I

S

S

G

P

S

G

P

P

A

C

S

T

V

P

L

D

E

E

D

A

G

G

S

I

P

S

R

L

V

D

P

V

Q

I

A

R

I

H

F

Y

E

A

S

G

G

R

L

L

P

P

V

I

L

L

G

G

I

V

C
|
C

Y

L

G

G

Q

H

Q

Q

V

A

M

M

M

A

Q

Q

Q

A

L

F

G

G

N

K

V

V

Q

V

L

R

G

A

D

A

S

K

G

V

E

M

F

H

G

G

R

K

A

T

F

S

I

P

E

I

I

T

A

H

D

N

S

G

C

E

V

G

L

V

F

F

D

R

G

G

L

L

W

-

A

A

E

N

G

P

E

L

T

T

H

V

Q

T

V

R

W

Y

M

H

S

S

H

L

G

V

D

V

K

E

V

P

T

D

S

S

L

L

A

P

P

A

G

C

F

F

R

D

P

V

V

T

A

A

A

W

S

S

A

E

G

T

A

R

P

E

F

I

A

M

V

G

I

I

A

R

D

H

D

R

T

Q

R

W

R

D

Y

L

Y

E

A

G

M

V

Q

Q

F

F

H
|
H

P

P

E
|
E

V

S

V

I

H

L

T

S

P

E

D

Q

G

G

A

H

K

Q

L

L

A

A

N

N

F

F

V

L

R

H

C79 (= C84) mutation to S: 10000-fold decrease in catalytic efficiency.
H168 (= H174) mutation to Q: Loss of activity.
E170 (= E176) mutation to A: 150-fold decrease in catalytic efficiency.; mutation to D: 4-fold decrease in catalytic efficiency.; mutation E->K,Q: Loss of activity.

7d54A Crstal structure msgatase with gln (see paper)
30% identity, 20% coverage: 75:177/518 of query aligns to 92:182/242 of 7d54A

query
sites
7d54A

E

D

S

A

G

G

L

V

P

G

V

I

L

L

G

G

I

V

C
|
C

Y

F

G

G

Q

G

Q

Q

V

L

M

L

M

A

Q

Q

Q

T

L

F

G

G

N

S

V

V

Q

A

L

R

G

A

D

E

S

T

G

A

E

E

F

V

G

G

R

W

A

F

F

E

I

L

E

D

I

T

A

D

D

D

S

A

C

G

V

L

L

I

F

A

D

P

G

G

L

P

W

W

A

F

E

Q

G

-

E

-

T

-

H

-

Q

-

V

-

W
|
W

M

-

S

-

H

H

G

F

D

D

K

R

V

W

T

T

S

-

L

V

A

P

P

P

G

G

F

A

R

T

P

E

V

I

A

A

A

R

S

T

A

S

G

R

A

S

P

S

F

Q

A

A

V

F

I

V

A

-

D

-

T

L

R

G

R

R

Y

A

Y

L

A

A

M

L

Q

Q

F

F

H

H

P

P

E

E

V

V

binding glutamine: C101 (= C84), W144 (= W133)

Sites not aligning to the query:

binding glutamine: 66, 190

Q9LVW7 Carbamoyl phosphate synthase small chain, chloroplastic; Carbamoyl phosphate synthetase glutamine chain; Protein VENOSA 6; EC 6.3.5.5 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
30% identity, 18% coverage: 2:96/518 of query aligns to 236:330/430 of Q9LVW7

query
sites
Q9LVW7

T

S

Q

R

P

D

G

G

E

K

S

S

I

Y

L

K

I

V

V

I

D

A

F

Y

G

D

S

F

Q

G

V

I

T

K

Q

Q

L

N

I

I

A

L

R

R

V

L

R

S

E

S

A

Y

G

G

V

C

Y

Q

S

I

E

T

I

V

A

V

P

P

F

S

N

T

A

F

A

P

A

A

E

A

A

E

F

A

E

L

R

K

M

M

K

N

P

P

G

D

G

G

V

I

I

L

L

F

S

S

G

N

S

G

P

P

A

-

S

-

V

-

L

-

E

-

D

-

G

G

S

D

P

P

R

S

-

A

V

V

P

P

Q

Y

A

A

I

V

-

E

-

T

-

V

-

K

-

E

F

L

E

L

S

G

G

K

L

V

P

P

V

V

L

Y

G

G

I

I

C

C

Y

M

G

G

Q

H

Q

Q

V

L

M

L

M

G

Q

Q

Q

A

L

L

G

G

Sites not aligning to the query:

410 H→Y: In ven6-1; reticulate leaf phenotype.

Query Sequence

>Ga0059261_0317 FitnessBrowser__Korea:Ga0059261_0317
MTQPGESILIVDFGSQVTQLIARRVREAGVYSEIAPFNAAAEAFERMKPGGVILSGSPAS
VLEDGSPRVPQAIFESGLPVLGICYGQQVMMQQLGGNVQLGDSGEFGRAFIEIADSCVLF
DGLWAEGETHQVWMSHGDKVTSLAPGFRPVAASAGAPFAVIADDTRRYYAMQFHPEVVHT
PDGAKLLANFVRHVCGLKGDWTMAEFRQTKIEEIRKQVGTGKVICGLSGGVDSAVAAVLI
HEAIGEQLTCVFVDHGLMRSGEADQVVSLFRGHYNIPLVHVNAETLFLNGLAGVTDPEAK
RKFIGKTFIDVFEEEAKKIGGAEFLAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGLPER
MNMKLVEPLRELFKDEVRALGRELGLPDVFVGRHPFPGPGLAIRIPGEVTKERCDILRKA
DAVYLEEIRNAGLYDAIWQAFAVLLPVKTVGVMGDGRTYDSVCGLRAVTSTDGMTADVYP
FDASFLTRVATRIVNEVKGVNRVVYDYTSKPPGTIEWE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory