SitesBLAST

Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
48% identity, 56% coverage: 21:211/343 of query aligns to 9:202/304 of Q96XT4

query
sites
Q96XT4

V

V

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W

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C

P

P

G

G

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C

G

G

D

D

Y

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G

I

I

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A

M

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E

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G

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I

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I

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C
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C

S
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S

S

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P

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Y

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N

-

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R

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N

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A

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N

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N

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x
V

Y

Y

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G

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L

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T

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K

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G

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A

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G

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C12 (= C24) binding [4Fe-4S] cluster
C15 (= C27) binding [4Fe-4S] cluster
IGCS 44:47 (= IGCS 56:59) binding thiamine diphosphate
C46 (= C58) binding [4Fe-4S] cluster
D90 (= D100) binding Mg(2+)
GD 91:92 (= GD 101:102) binding thiamine diphosphate
N118 (= N128) binding Mg(2+)
V120 (≠ I130) binding Mg(2+)
GL 122:123 (= GL 132:133) binding thiamine diphosphate
C197 (= C206) binding [4Fe-4S] cluster

5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
48% identity, 56% coverage: 21:211/343 of query aligns to 6:199/301 of 5b46B

query
sites
5b46B

V

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W

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C

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A

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G

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binding magnesium ion: D87 (= D100), N115 (= N128), V117 (≠ I130)
binding iron/sulfur cluster: W8 (= W23), C9 (= C24), C12 (= C27), C43 (= C58), C194 (= C206), T196 (≠ V208), Y197 (= Y209)
binding thiamine diphosphate: I41 (= I56), G42 (= G57), C43 (= C58), S44 (= S59), H62 (= H75), G86 (= G99), G88 (= G101), D89 (= D102), N115 (= N128), V117 (≠ I130), Y118 (= Y131), G119 (= G132), L120 (= L133), T121 (= T134)

Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
47% identity, 55% coverage: 23:211/343 of query aligns to 11:202/305 of Q96Y68

query
sites
Q96Y68

W

W

C
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C

P

P

G

G

C
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C

G

G

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N

Y

F

A

G

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N

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D

C12 (= C24) binding [4Fe-4S] cluster
C15 (= C27) binding [4Fe-4S] cluster
C46 (= C58) binding [4Fe-4S] cluster
K49 (≠ R61) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
D90 (= D100) binding Mg(2+)
GD 91:92 (= GD 101:102) binding thiamine diphosphate
N118 (= N128) binding Mg(2+)
V120 (≠ I130) binding Mg(2+)
GL 122:123 (= GL 132:133) binding thiamine diphosphate
K125 (= K135) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
K173 (= K182) mutation to A: Same oxidoreductase activity as the wild-type.
C197 (= C206) binding [4Fe-4S] cluster

P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
46% identity, 57% coverage: 17:211/343 of query aligns to 5:202/305 of P72579

query
sites
P72579

T

T

D

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N

R

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W

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C

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D

K49 (≠ R61) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
L123 (= L133) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.

5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
45% identity, 55% coverage: 21:209/343 of query aligns to 2:182/275 of 5b47B

query
sites
5b47B

V

V

R

D

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C

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C

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N
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N

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x
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-

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Y

A

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G

G

T

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R

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F

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A

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A

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A

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A

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A

G

G

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F

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V

A

A

R

R

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G

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Y

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A

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Y

D

D

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V

H

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K

M

N

H

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L

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K

A

K

A

A

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H

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K

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G

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A

A

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C

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Y

binding magnesium ion: D83 (= D100), N111 (= N128)
binding iron/sulfur cluster: C5 (= C24), C8 (= C27), C39 (= C58), C179 (= C206)
binding thiamine diphosphate: I37 (= I56), G38 (= G57), C39 (= C58), S40 (= S59), H58 (= H75), G84 (= G101), D85 (= D102), N111 (= N128), V113 (≠ I130), Y114 (= Y131), L116 (= L133)

5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
45% identity, 57% coverage: 17:211/343 of query aligns to 1:188/286 of 5b48B

query
sites
5b48B

T

T

D

P

Q

Q

E

W

V

N

R

D

W

W

C
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C

P

P

G

G

C
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C

G

G

D

N

Y

F

A

G

I

I

L

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K

N

A

A

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Q

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T

A

M

I

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E

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L

G

G

A

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D

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E

K

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N

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V

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S

S

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I

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G

C
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C

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S

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H
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H

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G

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R

A

A

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A

A

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A

A

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T

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K

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N

N

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D

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L

D

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G

G

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D

G
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G

D
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D

G

L

L

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G

I

I

G

G

G

A

N

G

H

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H

A

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A

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R

N

N

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N
|
N

E

G

I
x
V

Y
|
Y

G
|
G

L
|
L

T

T

K

K

G

G

Q

Q

Y

A

S

S

P

P

T

T

S

L

R

K

E

R

G

G

T

E

R

-

S

-

P

-

S

-

T

-

P

-

F

-

G

-

S

N

V

I

D

N

H

D

P

A

A

V

K

N

P

P

C

I

A

A

Y

L

A

A

L

I

G

S

S

S

G

G

A

Y

R

T

F

F

I

V

A

A

R

R

G

G

-

Y

-

A

I

Y

D

D

V

V

H

-

K

K

N

H

L

L

P

K

S

E

V

L

L

I

K

K

A

S

A

A

H

I

A

K

H

H

Q

K

G

G

A

L

A

A

F

L

V

I

E

D

I

V

F

L

Q

Q

N

P

C
|
C

I

P

V
x
T

Y

Y

N

N

D

D

binding magnesium ion: D84 (= D100), N112 (= N128), V114 (≠ I130)
binding iron/sulfur cluster: C8 (= C24), C11 (= C27), C42 (= C58), C183 (= C206), T185 (≠ V208)
binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I56), G41 (= G57), C42 (= C58), S43 (= S59), H59 (= H75), G85 (= G101), D86 (= D102), N112 (= N128), V114 (≠ I130), Y115 (= Y131), G116 (= G132), L117 (= L133)

5exeC Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-tpp adduct (see paper)
26% identity, 45% coverage: 1:156/343 of query aligns to 2:166/314 of 5exeC

query
sites
5exeC

V

L

N

D

E

R

I

I

T

A

T

S

I

I

K

K

P

K

S

A

T

P

P

D

K

E

D

E

W

Y

E

Y

T

V

D

P

Q

G

E

H

V

-

R

R

W

T

C
|
C

P

A

G

G

C
|
C

G

G

D
x
P

Y

A

A

L

I

T

L

Y

K

R

A

L

V

V

Q

A

R

K

T

-

M

-

P

-

E

-

I

-

G

-

A

A

T

A

P

G

E

P

N

N

T

T

V

I

F

F

I

I

S

G

G

P

I
x
T

G
|
G

C
|
C

S

M

-

Y

-

V

S

A

R

N

F

T

P

S

Y

Y

Y

G

M

C

E

G

T

P

Y

W

G

R

F

V

H

P

T

W

I

I

H

H

G

A

R

Q

A
x
I

P

T

-

N

-

G

A

A

V

V

A

A

T

S

G

G

V

I

K

E

L

A

A

A

N

Y

P

K

D

A

L

M

-

I

-

R

-

K

-

T

-

D

-

A

-

E

-

F

-

P

D

N

V

I

W

I

I

V

I

M

T

A

G
|
G

D
|
D

G
|
G

D

G

G

A

L

V

S

D

I

I

G

G

G

L

N

Q

H

A

T

L

M

S

H

A

L

M

L

L

R

Y

R

R

N

G

L

H

N
x
D

C

V

Q

L

V

F

L

I

L

C

F
x
Y

N

D

N
|
N

E

E

I
x
S

Y
|
Y

G
x
A

L
x
N

T
|
T

K

G

G

I

Q

Q

Y

T

S

S

P

P

T

T

S

T

R

P

E

Y

G

G

T

A

R

N

S

T

P

T

S

F

T

T

P

P

F

P

G

G

S

E

V

V

active site: N143 (≠ L133)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-carboxy-4-methyl-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (≠ I56), G51 (= G57), C52 (= C58), I74 (≠ A78), G109 (= G99), D110 (= D100), G111 (= G101), Y136 (≠ F126), N138 (= N128), S140 (≠ I130), Y141 (= Y131), A142 (≠ G132), N143 (≠ L133), T144 (= T134)
binding magnesium ion: D110 (= D100), D130 (≠ N120), N138 (= N128), S140 (≠ I130)
binding iron/sulfur cluster: C24 (= C24), C27 (= C27), P29 (≠ D29), C52 (= C58)

Sites not aligning to the query:

5exdF Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
26% identity, 45% coverage: 1:156/343 of query aligns to 2:166/310 of 5exdF

query
sites
5exdF

V

L

N

D

E

R

I

I

T

A

T

S

I

I

K

K

P

K

S

A

T

P

P

D

K

E

D

E

W

Y

E

Y

T

V

D

P

Q

G

E

H

V

-

R

R

W

T

C
|
C

P

A

G

G

C
|
C

G

G

D
x
P

Y

A

A

L

I

T

L

Y

K

R

A

L

V

V

Q

A

R

K

T

-

M

-

P

-

E

-

I

-

G

-

A

A

T

A

P

G

E

P

N

N

T

T

V

I

F

F

I

I

S

G

G

P

I
x
T

G
|
G

C
|
C

S

M

-

Y

-

V

S

A

R

N

F

T

P

S

Y

Y

Y

G

M

C

E

G

T

P

Y

W

G

R

F

V

H

P

T

W

I

I

H

H

G

A

R

Q

A
x
I

P

T

-

N

-

G

A

A

V

V

A

A

T

S

G

G

V

I

K

E

L

A

A

A

N

Y

P

K

D

A

L

M

-

I

-

R

-

K

-

T

-

D

-

A

-

E

-

F

-

P

D

N

V

I

W

I

I

V

I

M

T

A

G

G

D
|
D

G
|
G

D

G

G

A

L

V

S

D

I

I

G

G

G

L

N

Q

H

A

T

L

M

S

H

A

L

M

L

L

R

Y

R

R

N

G

L

H

N

D

C

V

Q

L

V

F

L

I

L

C

F
x
Y

N

D

N
|
N

E

E

I
x
S

Y
|
Y

G
x
A

L
x
N

T
|
T

K

G

G

I

Q

Q

Y

T

S

S

P

P

T

T

S

T

R

P

E

Y

G

G

T

A

R

N

S

T

P

T

S

F

T

T

P

P

F

P

G

G

S

E

V

V

active site: N143 (≠ L133)
binding magnesium ion: D110 (= D100), N138 (= N128), S140 (≠ I130)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-[1,1,2-tris(oxidanyl)-2-oxidanylidene-ethyl]-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (≠ I56), G51 (= G57), C52 (= C58), I74 (≠ A78), D110 (= D100), G111 (= G101), Y136 (≠ F126), N138 (= N128), S140 (≠ I130), Y141 (= Y131), A142 (≠ G132), N143 (≠ L133), T144 (= T134)
binding iron/sulfur cluster: C24 (= C24), C27 (= C27), P29 (≠ D29), C52 (= C58), A142 (≠ G132)

Sites not aligning to the query:

binding iron/sulfur cluster: 225, 227

5c4iC Structure of an oxalate oxidoreductase (see paper)
26% identity, 45% coverage: 1:156/343 of query aligns to 2:166/312 of 5c4iC

query
sites
5c4iC

V

L

N

D

E

R

I

I

T

A

T

S

I

I

K

K

P

K

S

A

T

P

P

D

K

E

D

E

W

Y

E

Y

T

V

D

P

Q

G

E

H

V

-

R

R

W

T

C
|
C

P

A

G

G

C
|
C

G

G

D
x
P

Y

A

A

L

I

T

L

Y

K

R

A

L

V

V

Q

A

R

K

T

-

M

-

P

-

E

-

I

-

G

-

A

A

T

A

P

G

E

P

N

N

T

T

V

I

F

F

I

I

S

G

G

P

I
x
T

G
|
G

C
|
C

S

M

-

Y

-

V

S

A

R

N

F

T

P

S

Y

Y

Y

G

M

C

E

G

T

P

Y

W

G

R

F

V

H

P

T

W

I

I

H

H

G

A

R

Q

A
x
I

P

T

-

N

-

G

A

A

V

V

A

A

T

S

G

G

V

I

K

E

L

A

A

A

N

Y

P

K

D

A

L

M

-

I

-

R

-

K

-

T

-

D

-

A

-

E

-

F

-

P

D

N

V

I

W

I

I

V

I

M

T

A

G
|
G

D
|
D

G
|
G

D

G

G

A

L

V

S

D

I

I

G

G

G

L

N

Q

H

A

T

L

M

S

H

A

L

M

L

L

R

Y

R

R

N

G

L

H

N

D

C

V

Q

L

V

F

L

I

L

C

F
x
Y

N

D

N
|
N

E

E

I
x
S

Y
|
Y

G
x
A

L
x
N

T
|
T

K

G

G

I

Q

Q

Y

T

S

S

P

P

T

T

S

T

R

P

E

Y

G

G

T

A

R

N

S

T

P

T

S

F

T

T

P

P

F

P

G

G

S

E

V

V

active site: N143 (≠ L133)
binding magnesium ion: D110 (= D100), N138 (= N128), S140 (≠ I130)
binding iron/sulfur cluster: C24 (= C24), C27 (= C27), P29 (≠ D29), C52 (= C58), A142 (≠ G132)
binding thiamine diphosphate: T50 (≠ I56), G51 (= G57), C52 (= C58), I74 (≠ A78), G109 (= G99), D110 (= D100), G111 (= G101), Y136 (≠ F126), N138 (= N128), S140 (≠ I130), Y141 (= Y131), A142 (≠ G132), N143 (≠ L133), T144 (= T134)

Sites not aligning to the query:

binding iron/sulfur cluster: 225, 226, 227

6cioA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with lactyl-tpp bound (see paper)
27% identity, 33% coverage: 94:207/343 of query aligns to 960:1075/1164 of 6cioA

query
sites
6cioA

V

I

W

W

I

I

T

G

G
|
G

D
|
D

G
|
G

D

W

G

A

L

Y

S

D

I

I

G

G

G

Y

N

G

H

G

T

L

M

D

H

H

L

V

L

L

R

A

R

S

N

G

L

A

N

N

C

V

Q

N

V

V

L

L

L

V

F

L

N

D

N
x
T

E

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

Y

S

S

S

P

K

T

A

S

T

R

Q

E

T

G

G

T

A

R

V

S

A

P

R

S

F

T

A

P

A

F

G

G

G

S

K

V

F

D

T

H

K

P

K

A

K

K

D

P

L

C

G

A

L

Y

M

A

A

L

M

G

S

S

Y

G

G

A

Y

R

V

F

Y

I

V

A

A

R

S

G

V

I

A

-

M

-

G

D

A

V

S

H

H

K

S

N

Q

L

L

P

M

S

K

V

A

L

L

K

I

A

E

A

A

H

E

A

K

H

Y

Q

D

G

G

A

P

A

S

F

L

V

I

E

I

I

A

F

Y

Q

A

N

P

C
|
C

I
|
I

active site: N999 (≠ L133)
binding magnesium ion: D966 (= D100), T994 (≠ N128), V996 (≠ I130)
binding iron/sulfur cluster: C1074 (= C206), I1075 (= I207)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: G965 (= G99), D966 (= D100), G967 (= G101), V996 (≠ I130), Y997 (= Y131), S998 (≠ G132), N999 (≠ L133), T1000 (= T134)

Sites not aligning to the query:

active site: 28, 61, 111
binding iron/sulfur cluster: 455, 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: 26, 27, 28, 61, 85, 111, 813, 834, 835, 836, 865

6cipA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with acetyl-tpp bound (see paper)
27% identity, 33% coverage: 94:207/343 of query aligns to 960:1075/1165 of 6cipA

query
sites
6cipA

V

I

W

W

I

I

T

G

G
|
G

D
|
D

G
|
G

D

W

G

A

L

Y

S

D

I

I

G

G

G

Y

N

G

H

G

T

L

M

D

H

H

L

V

L

L

R

A

R

S

N

G

L

A

N

N

C

V

Q

N

V

V

L

L

L

V

F

L

N

D

N
x
T

E

E

I
x
V

Y

Y

G
x
S

L
x
N

T

T

K

G

G

G

Q

Q

Y

S

S

S

P

K

T

A

S

T

R

Q

E

T

G

G

T

A

R

V

S

A

P

R

S

F

T

A

P

A

F

G

G

G

S

K

V

F

D

T

H

K

P

K

A

K

K

D

P

L

C

G

A

L

Y

M

A

A

L

M

G

S

S

Y

G

G

A

Y

R

V

F

Y

I

V

A

A

R

S

G

V

I

A

-

M

-

G

D

A

V

S

H

H

K

S

N

Q

L

L

P

M

S

K

V

A

L

L

K

I

A

E

A

A

H

E

A

K

H

Y

Q

D

G

G

A

P

A

S

F

L

V

I

E

I

I

A

F

Y

Q

A

N

P

C
|
C

I

I

active site: N999 (≠ L133)
binding 2-acetyl-thiamine diphosphate: G965 (= G99), D966 (= D100), G967 (= G101), V996 (≠ I130), S998 (≠ G132), N999 (≠ L133)
binding magnesium ion: D966 (= D100), T994 (≠ N128), V996 (≠ I130)
binding iron/sulfur cluster: S998 (≠ G132), C1074 (= C206)

Sites not aligning to the query:

active site: 28, 61, 111
binding 2-acetyl-thiamine diphosphate: 27, 61, 85, 111, 813, 834, 835, 836, 865
binding iron/sulfur cluster: 455, 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836

6ciqA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with coenzyme a bound (see paper)
27% identity, 33% coverage: 94:207/343 of query aligns to 960:1075/1169 of 6ciqA

query
sites
6ciqA

V

I

W

W

I

I

T

G

G
|
G

D
|
D

G
|
G

D

W

G

A

L

Y

S

D

I

I

G

G

G

Y

N

G

H

G

T

L

M

D

H

H

L

V

L

L

R

A

R

S

N

G

L

A

N

N

C

V

Q

N

V

V

L

L

L

V

F

L

N

D

N
x
T

E

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

Y

S

S

S

P

K

T

A

S

T

R

Q

E

T

G

G

T

A

R

V

S

A

P

R

S

F

T

A

P

A

F

G

G

G

S

K

V

F

D

T

H

K

P

K

A

K

K

D

P

L

C

G

A

L

Y

M

A

A

L

M

G

S

S

Y

G

G

A

Y

R

V

F

Y

I

V

A

A

R

S

G

V

I

A

-

M

-

G

D

A

V

S

H

H

K

S

N

Q

L

L

P

M

S

K

V

A

L

L

K

I

A

E

A

A

H

E

A

K

H

Y

Q

D

G

G

A

P

A

S

F

L

V

I

E

I

I

A

F

Y

Q

A

N

P

C
|
C

I
|
I

active site: N999 (≠ L133)
binding coenzyme a: N999 (≠ L133)
binding magnesium ion: D966 (= D100), T994 (≠ N128), V996 (≠ I130)
binding iron/sulfur cluster: S998 (≠ G132), C1074 (= C206), I1075 (= I207)
binding thiamine diphosphate: G965 (= G99), D966 (= D100), G967 (= G101), V996 (≠ I130), Y997 (= Y131), S998 (≠ G132), N999 (≠ L133), T1000 (= T134)

Sites not aligning to the query:

active site: 28, 61, 111
binding coenzyme a: 28, 423, 424, 427, 428, 553, 586
binding iron/sulfur cluster: 678, 680, 685, 686, 688, 689, 691, 695, 696, 700, 734, 741, 742, 743, 744, 745, 747, 751, 757, 807, 808, 811, 813, 836
binding thiamine diphosphate: 26, 27, 61, 85, 813, 835, 836, 865

6cinB Crystal structure of pyruvate:ferredoxin oxidoreductase from moorella thermoacetica (see paper)
27% identity, 33% coverage: 94:207/343 of query aligns to 960:1075/1169 of 6cinB

query
sites
6cinB

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active site: N999 (≠ L133)
binding magnesium ion: D966 (= D100), T994 (≠ N128), V996 (≠ I130)
binding iron/sulfur cluster: S998 (≠ G132), C1074 (= C206), I1075 (= I207)
binding thiamine diphosphate: G965 (= G99), D966 (= D100), G967 (= G101), V996 (≠ I130), Y997 (= Y131), S998 (≠ G132), N999 (≠ L133), T1000 (= T134)

Sites not aligning to the query:

active site: 28, 61, 111
binding iron/sulfur cluster: 455, 678, 685, 686, 687, 688, 689, 691, 695, 700, 741, 742, 743, 744, 745, 746, 747, 751, 752, 757, 758, 807, 808, 811, 813, 836
binding thiamine diphosphate: 26, 27, 61, 834, 835, 836, 865

Q2RMD6 Pyruvate:ferredoxin oxidoreductase; PFOR; Pyruvate synthase; EC 1.2.7.1 from Moorella thermoacetica (strain ATCC 39073 / JCM 9320) (see paper)
27% identity, 33% coverage: 94:207/343 of query aligns to 961:1076/1171 of Q2RMD6

query
sites
Q2RMD6

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D967 (= D100) binding Mg(2+)
DGW 967:969 (≠ DGD 100:102) binding thiamine diphosphate
T995 (≠ N128) binding Mg(2+)
TEVYSN 995:1000 (≠ NEIYGL 128:133) binding thiamine diphosphate
V997 (≠ I130) binding Mg(2+)
C1075 (= C206) binding [4Fe-4S] cluster

Sites not aligning to the query:

424:428 binding CoA
456 binding CoA
556 binding CoA
598 binding CoA
686 binding [4Fe-4S] cluster
689 binding [4Fe-4S] cluster
692 binding [4Fe-4S] cluster
696 binding [4Fe-4S] cluster
742 binding [4Fe-4S] cluster
745 binding [4Fe-4S] cluster
748 binding [4Fe-4S] cluster
752 binding [4Fe-4S] cluster
809 binding [4Fe-4S] cluster
812 binding [4Fe-4S] cluster
814 binding thiamine diphosphate
837 binding [4Fe-4S] cluster; binding thiamine diphosphate

Query Sequence

>Ga0059261_0324 FitnessBrowser__Korea:Ga0059261_0324
VNEITTIKPSTPKDWETDQEVRWCPGCGDYAILKAVQRTMPEIGATPENTVFISGIGCSS
RFPYYMETYGFHTIHGRAPAVATGVKLANPDLDVWIITGDGDGLSIGGNHTMHLLRRNLN
CQVLLFNNEIYGLTKGQYSPTSREGTRSPSTPFGSVDHPAKPCAYALGSGARFIARGIDV
HKNLPSVLKAAHAHQGAAFVEIFQNCIVYNDDVFAPFTEKANAGNQLWLEHGQPMLFAGG
SKGIALDTERLTLKTVDVVEGNWQAAGVIVHNVSNRAIAHMLVEMPFGPFPMALGVLYDD
PAPTFESAVVAQNLAASEGKVPDLQKLVSKGQTWMVDKEPHPM

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory