SitesBLAST

P0DPQ8 Aromatic O-demethylase, reductase subunit; NADH--hemoprotein reductase; EC 1.6.2.- from Amycolatopsis sp. (strain ATCC 39116 / 75iv2) (see paper)
40% identity, 92% coverage: 9:333/353 of query aligns to 2:330/334 of P0DPQ8

query
sites
P0DPQ8

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x
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F

C35 (= C42) binding
C40 (= C47) binding
C43 (= C50) binding
C75 (= C82) binding
RQYS 145:148 (≠ RDYS 152:155) binding
HVR 162:164 (≠ YIR 168:170) binding
VAT 170:172 (≠ KAS 176:178) binding
T215 (≠ S217) binding
F330 (= F333) binding

Sites not aligning to the query:

334 binding

1krhA X-ray structure of benzoate dioxygenase reductase (see paper)
30% identity, 90% coverage: 17:333/353 of query aligns to 15:334/337 of 1krhA

query
sites
1krhA

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C

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F

active site: C306 (≠ A305)
binding flavin-adenine dinucleotide: Y143 (= Y141), R155 (= R152), S156 (≠ D153), Y157 (= Y154), S158 (= S155), V171 (≠ Y168), V172 (≠ I169), G178 (= G175), K179 (= K176), M180 (≠ A177), S181 (= S178), T219 (≠ S217), E332 (≠ D331), F334 (= F333)
binding fe2/s2 (inorganic) cluster: M38 (≠ H40), D39 (≠ G41), C40 (= C42), R41 (= R43), G43 (= G45), C45 (= C47), G46 (= G48), C48 (= C50), C82 (= C82)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 335, 336, 337

4wqmA Structure of the toluene 4-monooxygenase nadh oxidoreductase t4mof, k270s k271s variant (see paper)
33% identity, 88% coverage: 24:334/353 of query aligns to 18:326/326 of 4wqmA

query
sites
4wqmA

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F

F

F

binding flavin-adenine dinucleotide: C36 (= C42), S38 (= S44), W57 (≠ A63), Y135 (= Y141), R146 (= R152), A147 (≠ D153), Y148 (= Y154), S149 (= S155), I162 (≠ Y168), V163 (≠ I169), G169 (= G175), K170 (= K176), V171 (≠ A177), S172 (= S178), S209 (= S217), F325 (= F333)
binding fe2/s2 (inorganic) cluster: Y34 (≠ H40), C36 (= C42), N37 (≠ R43), G39 (= G45), C41 (= C47), G42 (= G48), C44 (= C50), C76 (= C82)

Q03304 Toluene-4-monooxygenase system, ferredoxin--NAD(+) reductase component; T4MO; Ferredoxin--NAD(+) reductase; Toluene-4-monooxygenase systme, electron transfer component; EC 1.18.1.3 from Pseudomonas mendocina (see paper)
33% identity, 88% coverage: 26:334/353 of query aligns to 20:326/326 of Q03304

query
sites
Q03304

T

T

I

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A

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A

A

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A

A

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C

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C

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M

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L

Y

-

L

-

V

-

D

E

H

A

F

A

E

E

R

T

L

L

A

F

A

G

A

W

H

K

A

E

N

N

F

L

H

K

F

L

E

I

P

N

V

V

L

S

S

S

R

S

E

V

S

V

D

G

A

N

G

S

A

E

G

K

R

K

-

Y

-

P

A

T

G

G

F

Y

V

V

T

H

D

E

A

I

I

I

A

P

A

E

D

Y

F

M

T

E

D

G

L

L

D

L

G

G

W

A

K

E

A

F

Y

Y

L

L

A

C

G

G

P

P

A

Q

M

M

I

I

D

N

T

S

A

V

G

Q

P

K

L

L

K

M

A

I

R

E

G

N

-

K

L

V

A

P

S

F

E

E

D

A

F

I

H

H

A

F

D

D

I

R

F

F

C36 (= C42) binding
C41 (= C47) binding
C44 (= C50) binding
C76 (= C82) binding
RAYS 146:149 (≠ RDYS 152:155) binding
IVK 162:164 (≠ YIR 168:170) binding
KVS 170:172 (≠ KAS 176:178) binding

7c3bC Ferredoxin reductase in carbazole 1,9a-dioxygenase (fad apo form) (see paper)
30% identity, 90% coverage: 18:334/353 of query aligns to 16:334/334 of 7c3bC

query
sites
7c3bC

P

P

I

G

A

T

V

C

G

G

D

S

K

D

Q

K

T

S

I

L

L

L

A

V

A

S

A

A

L

L

D

A

A

N

G

G

V

I

A

G

F

L

P

P

H
x
Y

G

E

C
|
C

R
x
A

S

S

G
|
G

R

G

C
|
C

G
|
G

A

V

C
|
C

K

K

S

F

R

E

L

L

S

E

G

G

E

T

V

V

D

Q

L

S

L

M

A

W

H

P

T

D

R

A

F

P

A

G

L

L

T

S

A

S

E

R

E

D

K

R

A

E

Q

K

G

G

L

N

I

R

-

H

L

L

A

A

C
|
C

R

Q

A

C

Q

I

P

A

L

L

T

S

D

D

I

L

E

R

V

I

A

K

W

V

L

A

G

V

E

Q

A

D

E

K

I

Y

I

I

E

P

H

A

-

I

P

P

V

I

R

S

S

K

L

M

K

E

A

A

E

E

V

V

A

A

L

V

D

R

S

A

A

L

T

T

H

H

D

D

I

L

N

L

L

S

V

V

R

K

V

L

R

R

V

-

A

T

G

D

E

V

P

P

L

A

V

N

F

F

T

L

A

P

G

G

Q

Q

Y

F

A

C

Q

L

L

I

S

E

A

A

A

E

G

Q

A

L

P

P

-

G

-

V

T

V

R

R

D

A

Y

Y

S

S

M

M

A

A

N

N

V

S

P

M

G

N

E

P

E

D

D

G

L

F

-

W

E

E

F

F

Y

Y

I

I

R

K

H

R

I

V

P

P

G

T

G

G

K

R

A

F

S

S

E

P

R

W

I

L

A

F

T

E

H

N

L

R

A

K

V

V

G

G

D

A

T

R

L

L

T

F

L

L

R

T

G

G

P

P

F

M

G

G

S

T

A

S

Y

F

L

F

R

R

E

P

G

G

H

T

T

G

G

R

P

K

I

S

L

L

A

C

V

I

A

G

G

G

S

A

G

G

L

L

A

S

P

Y

I

A

K

A

A

A

I

I

V

A

E

R

T

A

A

S

L

I

E

R

K

E

G

-

L

T

R

D

Q

K

P

P

I

V

H

K

L

L

Y

F

F

Y

G

G

A

S

R

R

R

T

R

P

A

R

D

D

-

A

L

V

Y

R

L

W

V

I

D

D

H

-

F

-

E

-

R

-

L

I

A

D

A

I

A

D

H

E

A

D

N

K

F

L

H

E

F

V

E

V

P

Q

V

A

L

V

S

T

R

E

E

D

S

T

D

D

A

S

-

L

G

W

A

Q

G

G

R

P

A

I

G

G

F

F

V

I

T

H

D

Q

A

V

I

V

-

D

A

A

D

L

F

L

T

E

D

T

L

L

D

P

G

E

W

Y

K

E

A

I

Y

Y

L

L

A

A

G

G

P

P

A

P

M

M

I

V

D

D

T

A

A

T

G

V

P

R

L

M

L

L

K

L

A

G

R

K

G

G

L

V

A

P

S

R

E

D

D

Q

F

I

H

H

A

F

D

D

I

A

F

F

binding fe2/s2 (inorganic) cluster: Y38 (≠ H40), C40 (= C42), A41 (≠ R43), G43 (= G45), C45 (= C47), G46 (= G48), C48 (= C50), C81 (= C82)

7c3aA Ferredoxin reductase in carbazole 1,9a-dioxygenase (see paper)
30% identity, 90% coverage: 18:334/353 of query aligns to 15:321/321 of 7c3aA

query
sites
7c3aA

P

P

I

G

A

T

V

C

G

G

D

S

K

D

Q

K

T

S

I

L

L

L

A

V

A

S

A

A

L

L

D

A

A

N

G

G

V

I

A

G

F

L

P

P

H
x
Y

G

E

C
|
C

R
x
A

S
|
S

G
|
G

R

G

C
|
C

G
|
G

A

V

C
|
C

K

K

S

F

R

E

L

L

S

E

G

G

E

T

V

V

D

Q

L

S

L

M

A
x
W

H

P

T

D

R

A

F

P

A

G

L

L

T

S

A

S

E

R

E

D

K

R

A

E

Q

K

G

G

-

N

L

R

I

H

L

L

A

A

C
|
C

R

Q

A

C

Q

I

P

A

L

L

T

S

D

D

I

L

E

R

V

I

A

K

W

V

L

A

G

V

E

Q

A

D

E

K

I

Y

I

I

E

P

H

A

-

I

P

P

V

I

R

S

S

K

L

M

K

E

A

A

E

E

V

V

A

A

L

V

D

R

S

A

A

L

T

T

H

H

D

D

I

L

N

L

L

S

V

V

R

K

V

L

R

R

V

-

A

T

G

D

E

V

P

P

L

A

V

N

F

F

T

L

A

P

G

G

Q

Q

Y

F

A

C

Q

L

L

I

S

E

A

A

A

E

G

Q

A

L

P

P

-

G

-

V

T

V

R
|
R

D
x
A

Y
|
Y

S
|
S

M

M

A

A

N

N

V

S

P

M

G

N

E

P

E

D

D

G

L

F

-

W

E

E

F

F

Y
|
Y

I
|
I

R

K

H

R

I

V

P

P

G

T

G
|
G

K
x
R

A
x
F

S
|
S

E

P

R

W

I

L

A

F

T

E

H

N

L

R

A

K

V

V

G

G

D

A

T

R

L

L

T

F

L

L

R

T

G

G

P

P

F

M

G

G

S

T

A

S

Y

F

L

F

R

R

E

P

G

G

H

T

T

G

G

R

P

K

I

S

L

L

A

C

V

I

A

G

G

G

S
x
A

G

G

L

L

A

S

P

Y

I

A

K

A

A

A

I

I

V

A

E

R

T

A

A

S

L

I

E

R

K

E

G

-

L

T

R

D

Q

K

P

P

I

V

H

K

L

L

Y

F

F

Y

G

G

A

S

R

R

R

T

R

P

A

R

D

D

-

A

L

V

Y

R

L

W

V

I

D

D

-

I

-

D

-

I

H

D

F

E

E

D

R

K

L

L

A

E

A

V

H

Q

A

A

N

V

F

T

H

F

F

I

E

H

P

Q

V

V

L

V

S

D

R

-

E

-

S

-

D

-

A

-

G

-

A

-

G

-

R

-

A

-

G

-

F

-

V

-

T

-

D

-

A

-

I

-

A

A

D

L

F

L

T

E

D

T

L

L

D

P

G

E

W

Y

K

E

A

I

Y

Y

L

L

A

A

G

G

P

P

A

P

M

M

I

V

D

D

T

A

A

T

G

V

P

R

L

M

L

L

K

L

A

G

R

K

G

G

L

V

A

P

S

R

E

D

D

Q

F

I

H

H

A

F

D

D

I

A

F
|
F

binding flavin-adenine dinucleotide: C39 (= C42), S41 (= S44), W60 (≠ A63), R152 (= R152), A153 (≠ D153), Y154 (= Y154), S155 (= S155), Y169 (= Y168), I170 (= I169), G176 (= G175), R177 (≠ K176), F178 (≠ A177), S179 (= S178), A218 (≠ S217), F320 (= F333), F321 (= F334)
binding fe2/s2 (inorganic) cluster: Y37 (≠ H40), C39 (= C42), A40 (≠ R43), G42 (= G45), C44 (= C47), G45 (= G48), C47 (= C50), C80 (= C82)

7c3bB Ferredoxin reductase in carbazole 1,9a-dioxygenase (fad apo form) (see paper)
30% identity, 90% coverage: 18:334/353 of query aligns to 15:306/306 of 7c3bB

query
sites
7c3bB

P

P

I

G

A

T

V

C

G

G

D

S

K

D

Q

K

T

S

I

L

L

L

A

V

A

S

A

A

L

L

D

A

A

N

G

G

V

I

A

G

F

L

P

P

H
x
Y

G

E

C
|
C

R
x
A

S

S

G
|
G

R

G

C
|
C

G
|
G

A

V

C
|
C

K

K

S

F

R

E

L

L

S

E

G

G

E

T

V

V

D

Q

L

S

L

M

A

W

H

P

T

D

R

A

F

P

A

G

L

L

T

S

A

S

E

R

E

D

K

R

A

E

Q

K

G

G

-

N

L

R

I

H

L

L

A

A

C
|
C

R

Q

A

C

Q

I

P

A

L

L

T

S

D

D

I

L

E

R

V

I

A

K

W

V

L

A

G

V

E

Q

A

D

E

K

I

Y

I

I

E

P

H

A

-

I

P

P

V

I

R

S

S

K

L

M

K

E

A

A

E

E

V

V

A

A

L

V

D

R

S

A

A

L

T

T

H

H

D

D

I

L

N

L

L

S

V

V

R

K

V

L

R

R

V

-

A

T

G

D

E

V

P

P

L

A

V

N

F

F

T

L

A

P

G

G

Q

Q

Y

F

A

C

Q

L

L

I

S

E

A

A

A

E

G

Q

A

L

P

P

-

G

-

V

T

V

R

R

D

A

Y

Y

S

S

M

M

A

A

N

N

V

S

P

M

G

N

E

P

E

D

D

G

L

F

-

W

E

E

F

F

Y

Y

I

I

R

K

H

R

I

V

P

P

G

-

K

-

A

F

S

S

E

P

R

W

I

L

A

F

T

E

H

N

L

R

A

K

V

V

G

G

D

A

T

R

L

L

T

F

L

L

R

T

G

G

P

P

F

M

G

G

S

T

A

S

Y

F

L

F

R

R

E

P

G

G

H

T

T

G

G

R

P

K

I

S

L

L

A

C

V

I

A

G

G

G

S

A

G

G

L

L

A

S

P

Y

I

A

K

A

A

A

I

I

V

A

E

R

T

A

A

S

L

I

E

R

K

E

G

-

L

-

R

-

Q

T

P

D

I

V

H

K

L

L

Y

F

F

Y

G

G

A

S

R

R

R

T

R

P

A

-

D

-

L

-

Y

-

L

W

V

I

D

D

H

I

F

D

E

E

R

V

L

V

A

Q

A

A

A

V

H

T

A

E

N

F

F

I

H

H

F

-

E

-

P

-

V

-

L

-

S

-

R

-

E

-

S

-

D

-

A

-

G

-

A

-

G

-

R

-

A

-

G

-

F

Q

V

V

T

V

D

D

A

-

I

-

A

A

D

L

F

L

T

E

D

T

L

L

D

P

G

E

W

Y

K

E

A

I

Y

Y

L

L

A

A

G

G

P

P

A

P

M

M

I

V

D

D

T

A

A

T

G

V

P

R

L

M

L

L

K

L

A

G

R

K

G

G

L

V

A

P

S

R

E

D

D

Q

F

I

H

H

A

F

D

D

I

A

F

F

binding fe2/s2 (inorganic) cluster: Y37 (≠ H40), C39 (= C42), A40 (≠ R43), G42 (= G45), C44 (= C47), G45 (= G48), C47 (= C50), C80 (= C82)

1tvcA Fad and nadh binding domain of methane monooxygenase reductase from methylococcus capsulatus (bath) (see paper)
32% identity, 65% coverage: 105:334/353 of query aligns to 8:245/250 of 1tvcA

query
sites
1tvcA

V

V

R

G

S

S

L

F

K

E

A

A

E

E

V

V

A

G

L

L

D

N

S

W

A

V

T

S

H

S

D

-

I

-

N
|
N

L

T

V

V

R

Q

V

F

R

L

V

L

A

Q

G

K

E

R

P

P

-

D

-

E

-

C

-

G

-

N

-

R

-

G

L

V

V

K

F

F

T

E

A

P

G

G

Q

Q

Y
x
F

A

M

Q

D

L

L

S

T

A

I

A

P

G

G

A

T

P

D

-

V

T

S

R
|
R

D
x
S

Y
|
Y

S
|
S

M

P

A

A

N

N

V

L

P

P

G

N

E

P

E

E

D

G

-

R

L

L

E

E

F

F

Y
x
L

I
|
I

R
|
R

H

V

I
x
L

P

P

G

E

G

G

K

R

A
x
F

S

S

E

D

R

Y

I

L

A

R

T

N

H

D

L

A

A

R

V

V

G

G

D

Q

T

V

L

L

T

S

L

V

R

K

G

G

P

P

F

L

G

G

S

V

A

F

Y

G

L

L

R

K

E

E

G

R

H

G

T

M

G

A

P

P

I

R

L

Y

A

F

V

V

A

A

G

G

S

T

G
|
G

L

L

A

A

P

P

I

V

K

V

A

S

I

M

V

V

E

R

T

Q

A

M

L

Q

E

E

K

W

G

T

L

A

R

P

Q

N

P

E

I

T

H

R

L

I

Y

Y

F

F

G

G

A

V

R
x
N

R

T

R

E

A

P

D
x
E

L

L

Y

F

L

Y

V

I

D

D

H

E

F

L

E

K

R

S

L

L

A

E

A

R

A

S

H

M

A

R

N

N

F

L

H

T

F

V

E

K

P

A

V

C

L

V

S

W

R

H

E

P

S
|
S

D
x
G

A

D

G

W

A

E

G

G

R

E

A

Q

G

G

F

S

V

P

T

I

D

D

A

A

I

L

A

R

A

E

D

D

F

L

T

E

D

S

L

S

D

D

-

A

G

N

W

P

K

D

A

I

Y

Y

L
|
L

A

C

G

G

P

P

A

G

M

M

I

I

D

D

T

A

A

A

G

C

P

E

L

L

L

V

K

R

A

S

R

R

G

G

L

I

A

P

S

G

E

E

D

Q

F

V

H

F

A

F

D
x
E

I

K

F
|
F

F
x
L

active site: Y63 (= Y154), S64 (= S155), L215 (= L304)
binding dihydroflavine-adenine dinucleotide: N24 (= N123), F49 (≠ Y141), R61 (= R152), S62 (≠ D153), Y63 (= Y154), S64 (= S155), L78 (≠ Y168), I79 (= I169), R80 (= R170), L82 (≠ I172), F87 (≠ A177), G128 (= G218), N155 (≠ R245), E159 (≠ D249), S186 (= S276), G187 (≠ D277), E242 (≠ D331), F244 (= F333), L245 (≠ F334)

7qu0A X-ray structure of fad domain of nqrf of klebsiella pneumoniae (see paper)
26% identity, 65% coverage: 104:333/353 of query aligns to 2:279/279 of 7qu0A

query
sites
7qu0A

P

P

V

V

R

K

S

K

L

W

K

E

A

C

E

E

V

V

V

I

A

S

L

N

D

D

S

N

A

K

T

A

H

T

D

F

I

I

N

K

L

E

V

L

R

K

V

L

R

R

V

I

-

P

A

E

G

G

E

E

P

V

L

V

V

P

F

F

T

R

A

A

G

G

Q

G

Y
|
Y

A

I

Q

Q

L

I

-

E

-

C

-

P

-

H

-

K

-

V

-

A

-

Y

-

A

-

D

-

F

-

D

-

V

-

P

-

D

-

E

-

Y

-

R

-

S

-

D

-

W

-

D

-

K

-

F

-

N

-

L

-

F

-

R

-

Y

-

V

S

S

A

E

A

V

G

K

A

E

P

P

T

T

-

L

R
|
R

D
x
A

Y
|
Y

S
|
S

M

M

A

A

N

N

V

Y

P

P

G

E

E

E

E

K

D

G

L

I

E

I

F

M

Y

L

I

N

R

V

H

R

I

I

-
x
A

-
x
T

-
x
P

-

P

-

K

-

I

-

P

-

D

-
x
A

P
|
P

G
x
P

G
|
G

K
x
I

A
x
M

S
|
S

E

S

R

Y

I

I

A

W

T

S

H

-

L

L

A

K

V

P

G

G

D

D

T

K

L

V

T

T

L

I

R

S

G

G

P

P

F

F

G

G

S

E

A

F

Y

F

L

A

R

K

E

E

G

T

H

-

T

D

G

A

P

E

I

M

L

V

A

F

V

I

A

G

G

G

S

A

G

G

L

M

A

A

P

P

I

M

K

R

A

S

I

H

V

I

E

F

T

D

A

Q

L

L

E

K

K

R

-

L

G

H

L

S

R

T

Q

R

P

K

I

I

H

S

L

F

Y

W

F

Y

G

G

A

A

R

R

R

S

R

L

A

R

D

E

L

M

Y

F

L

Y

V

D

D

E

H

E

F

F

E

E

R

Q

L

L

A

A

A

R

A

D

H

N

A

P

N

N

F

F

H

T

F

F

E

H

P

V

V

A

L

L

S
|
S

R

D

-

P

-

L

E

P

S

E

D

D

A

N

G

W

A

T

G

G

R

H

A

T

G

G

F
|
F

V

I

T

H

D

N

A

V

I

L

A

Y

A

E

D

N

F

Y

T

L

D

R

-

D

-

H

-

P

-

A

L

P

D

E

G

D

W

C

K

E

A

F

Y

Y

L

M

A

C

G

G

P

P

A

V

M
|
M

I

N

D

A

T

A

A

V

G

I

P

K

L

M

L

L

K

K

A

D

R

L

G

G

L

V

A

E

S

D

E

E

D

N

F

I

H

L

A

L

D

D

I

D

F
|
F

binding ~{N}-[2,6-bis(fluoranyl)phenyl]ethanamide: S213 (= S273), F227 (= F285), M256 (= M310)
binding flavin-adenine dinucleotide: Y40 (= Y141), R83 (= R152), A84 (≠ D153), Y85 (= Y154), S86 (= S155), A104 (vs. gap), T105 (vs. gap), P106 (vs. gap), P107 (vs. gap), A113 (vs. gap), P114 (= P173), P115 (≠ G174), G116 (= G175), I117 (≠ K176), M118 (≠ A177), S119 (= S178), F279 (= F333)

7qtyA X-ray structure of fad domain of nqrf of klebsiella pneumoniae (see paper)
26% identity, 65% coverage: 104:333/353 of query aligns to 2:279/279 of 7qtyA

query
sites
7qtyA

P

P

V

V

R

K

S

K

L

W

K

E

A

C

E

E

V

V

V

I

A

S

L

N

D

D

S

N

A

K

T

A

H

T

D

F

I

I

N

K

L

E

V

L

R

K

V

L

R

R

V

I

-

P

A

E

G

G

E

E

P

V

L

V

V

P

F

F

T

R

A

A

G

G

Q

G

Y
|
Y

A

I

Q

Q

L

I

-

E

-

C

-

P

-

H

-

K

-

V

-

A

-

Y

-

A

-

D

-

F

-

D

-

V

-

P

-

D

-

E

-

Y

-

R

-

S

-

D

-

W

-

D

-

K

-

F

-

N

-

L

-

F

-

R

-

Y

-

V

S

S

A

E

A

V

G

K

A

E

P

P

T

T

-

L

R
|
R

D
x
A

Y
|
Y

S
|
S

M

M

A

A

N

N

V

Y

P

P

G

E

E

E

E

K

D

G

L

I

E

I

F

M

Y

L

I
x
N

R

V

H

R

I

I

-
x
A

-
x
T

-

P

-
x
P

-

P

-

K

-

I

-

P

-

D

-
x
A

P
|
P

G

P

G
|
G

K
x
I

A
x
M

S
|
S

E

S

R

Y

I

I

A

W

T

S

H

-

L

L

A

K

V

P

G

G

D

D

T

K

L

V

T

T

L

I

R

S

G

G

P

P

F

F

G

G

S

E

A

F

Y

F

L

A

R

K

E

E

G

T

H

-

T

D

G

A

P

E

I

M

L

V

A

F

V

I

A

G

G

G

S

A

G

G

L

M

A

A

P

P

I

M

K

R

A

S

I

H

V

I

E

F

T

D

A

Q

L

L

E

K

K

R

-

L

G

H

L

S

R

T

Q

R

P

K

I

I

H

S

L

F

Y

W

F

Y

G

G

A
|
A

R
|
R

R

S

R

L

A

R

D

E

L

M

Y

F

L

Y

V

D

D

E

H

E

F

F

E

E

R

Q

L

L

A

A

A

R

A

D

H

N

A

P

N

N

F

F

H

T

F

F

E

H

P

V

V

A

L

L

S
|
S

R

D

-

P

-

L

E

P

S

E

D

D

A

N

G

W

A

T

G

G

R

H

A

T

G

G

F
|
F

V

I

T
x
H

D

N

A

V

I

L

A

Y

A

E

D

N

F

Y

T

L

D

R

-

D

-

H

-

P

-

A

L

P

D

E

G

D

W

C

K

E

A

F

Y

Y

L

M

A

C

G

G

P

P

A
x
V

M
|
M

I

N

D

A

T

A

A

V

G

I

P

K

L

M

L

L

K

K

A

D

R

L

G

G

L

V

A

E

S

D

E

E

D

N

F

I

H

L

A

L

D

D

I

D

F
|
F

binding 1-(furan-2-ylmethyl)-3-(2-methylphenyl)thiourea: A184 (= A244), R185 (= R245), S213 (= S273), F227 (= F285), H229 (≠ T287), V255 (≠ A309), M256 (= M310)
binding flavin-adenine dinucleotide: Y40 (= Y141), R83 (= R152), A84 (≠ D153), Y85 (= Y154), S86 (= S155), N100 (≠ I169), A104 (vs. gap), T105 (vs. gap), P107 (vs. gap), A113 (vs. gap), P114 (= P173), G116 (= G175), I117 (≠ K176), M118 (≠ A177), S119 (= S178), F279 (= F333)

8a1yF Sodium pumping nadh-quinone oxidoreductase with inhibitor hqno (see paper)
23% identity, 80% coverage: 51:333/353 of query aligns to 80:406/408 of 8a1yF

query
sites
8a1yF

K

R

S

V

R

K

L

I

L

K

S

S

G

G

E

G

V

G

D

D

L

I

L

L

A

P

H

T

T

E

R

L

F

D

A

H

L

I

T

S

A

K

E

G

E

E

K

A

A

R

Q

E

G

G

L

E

I

R

L

L

A

A

C
|
C

R

Q

A

V

Q

A

P

V

L

K

T

A

D

D

I

M

E

D

V

L

A

E

W

L

L

P

G

E

E

E

A

I

E

-

I

-

E

-

H

F

P

G

V

V

R

K

S

K

L

W

K

E

A

C

E

T

V

V

V

I

A

S

L

N

D

D

S

N

A

K

T

A

H

T

D

F

I

I

N

K

L

E

V

L

R

K

V

L

R

A

V

I

A

P

-

D

G

G

E

E

P

S

L

V

V

P

F

F

T

R

A

A

G

G

Q

G

Y

Y

A

I

Q

Q

L

I

S

E

A

A

A

P

G

A

-

H

-

V

-

K

-

Y

-

A

-

D

-

F

-

D

A

V

P

P

-

E

-

K

-

Y

-

R

-

G

-

D

-

W

-

D

-

K

-

F

-

N

-

L

-

F

-

R

-

Y

-

E

-

S

-

K

-

V

-

D

-

E

-

P

-

I

T

I

R
|
R

D
x
A

Y
|
Y

S
|
S

M

M

A

A

N

N

V

Y

P

P

G

E

E

E

E

F

D

G

L

I

E

I

F

M

Y

L

I

N

R
x
V

H
x
R

I

I

P
x
A

-

T

-

P

-

N

-

P

-

N

-
x
V

-
x
P

G

P

G
|
G

K
x
Q

A
x
M

S

S

E

S

R

Y

I

I

A

W

T

S

H

-

L

L

A

K

V

A

G

G

D

D

T

K

L

C

T

T

L

I

R

S

G

G

P

P

F

F

G

G

S

E

A

F

Y

F

L

A

R

K

E

D

G

-

H

T

T

D

G

A

P

E

I

M

L

V

A

F

V

I

A

G

G

G

S

A

G

G

L

M

A

A

P

P

I

M

K

R

A

S

I

H

V

I

E

F

T

D

A

Q

L

L

E

K

K

R

-

L

G

K

L

S

R

K

Q

R

P

K

I

M

H

S

L

Y

Y

W

F

Y

G

G

A

A

R

R

R

S

R

K

A

R

D

E

L

M

Y

F

L

Y

V

V

D

E

H

D

F

F

E

D

R

G

L

L

A

A

A

E

H

N

A

D

N

N

F

F

H

V

F

W

E

H

P

C

V

A

L

L

S

S

-

D

-

P

R

Q

E

P

S

E

D

D

A

N

G

W

A

T

G

G

R

Y

A

T

G

G

F

F

V

I

T

H

D

N

A

V

I

L

A

Y

A

E

D

N

F

Y

T

L

D

K

-

D

-

H

-

E

-

A

L

P

D

E

G

D

W

C

K

E

A

Y

Y

Y

L

M

A

C

G

G

P

P

A

M

M

M

I

N

D

A

T

A

A

V

G

I

P

N

L

M

L

L

K

K

A

N

R

L

G

G

L

V

A

E

S

E

E

E

D

N

F

I

H

L

A

L

D

D

I

D

F

F

binding flavin-adenine dinucleotide: R210 (= R152), A211 (≠ D153), Y212 (= Y154), S213 (= S155), V228 (≠ R170), R229 (≠ H171), A231 (≠ P173), V240 (vs. gap), P241 (vs. gap), G243 (= G175), Q244 (≠ K176), M245 (≠ A177)
binding fe2/s2 (inorganic) cluster: C111 (= C82)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 70, 74, 76, 77, 79

A5F5Y4 Na(+)-translocating NADH-quinone reductase subunit F; Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F; EC 7.2.1.1 from Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) (see paper)
23% identity, 80% coverage: 51:333/353 of query aligns to 80:406/408 of A5F5Y4

query
sites
A5F5Y4

K

R

S

V

R

K

L

I

L

K

S

S

G

G

E

G

V

G

D

D

L

I

L

L

A

P

H

T

T

E

R

L

F

D

A

H

L

I

T

S

A

K

E

G

E

E

K

A

A

R

Q

E

G

G

L

E

I

R

L

L

A

A

C
|
C

R

Q

A

V

Q

A

P

V

L

K

T

A

D

D

I

M

E

D

V

L

A

E

W

L

L

P

G

E

E

E

A

I

E

-

I

-

E

-

H

F

P

G

V

V

R

K

S

K

L

W

K

E

A

C

E

T

V

V

V

I

A

S

L

N

D

D

S

N

A

K

T

A

H

T

D

F

I

I

N

K

L

E

V

L

R

K

V

L

R

A

V

I

A

P

-

D

G

G

E

E

P

S

L

V

V

P

F

F

T

R

A

A

G

G

Q

G

Y

Y

A

I

Q

Q

L

I

S

E

A

A

A

P

G

A

-

H

-

V

-

K

-

Y

-

A

-

D

-

F

-

D

A

V

P

P

-

E

-

K

-

Y

-

R

-

G

-

D

-

W

-

D

-

K

-

F

-

N

-

L

-

F

-

R

-

Y

-

E

-

S

-

K

-

V

-

D

-

E

-

P

-

I

T

I

R
|
R

D

A

Y
|
Y

S

S

M

M

A

A

N

N

V

Y

P

P

G

E

E

E

E

F

D

G

L

I

E

I

F

M

Y

L

I

N

R

V

H

R

I

I

P

A

-

T

-

P

-

N

-

P

-

N

-

V

-

P

G

P

G

G

K

Q

A

M

S
|
S

E

S

R

Y

I

I

A

W

T

S

H

-

L

L

A

K

V

A

G

G

D

D

T

K

L

C

T

T

L

I

R

S

G

G

P

P

F

F

G

G

S

E

A

F

Y

F

L

A

R

K

E

D

G

-

H

T

T

D

G

A

P

E

I

M

L

V

A

F

V

I

A

G

G

G

S

A

G

G

L

M

A

A

P

P

I

M

K

R

A

S

I

H

V

I

E

F

T

D

A

Q

L

L

E

K

K

R

-

L

G

K

L

S

R

K

Q

R

P

K

I

M

H

S

L

Y

Y

W

F

Y

G

G

A

A

R

R

R

S

R

K

A

R

D

E

L

M

Y

F

L

Y

V

V

D

E

H

D

F

F

E

D

R

G

L

L

A

A

A

E

H

N

A

D

N

N

F

F

H

V

F

W

E

H

P

C

V

A

L

L

S

S

-

D

-

P

R

Q

E

P

S

E

D

D

A

N

G

W

A

T

G

G

R

Y

A

T

G

G

F

F

V

I

T

H

D

N

A

V

I

L

A

Y

A

E

D

N

F

Y

T

L

D

K

-

D

-

H

-

E

-

A

L

P

D

E

G

D

W

C

K

E

A

Y

Y

Y

L

M

A

C

G

G

P

P

A

M

M

M

I

N

D

A

T

A

A

V

G

I

P

N

L

M

L

L

K

K

A

N

R

L

G

G

L

V

A

E

S

E

E

E

D

N

F

I

H

L

A

L

D

D

I

D

F

F

C111 (= C82) binding ; mutation to A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
R210 (= R152) mutation to L: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.
Y212 (= Y154) mutation to L: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.
S246 (= S178) mutation to A: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.

Sites not aligning to the query:

70 binding ; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
76 binding ; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
79 binding ; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.

8a1uF Sodium pumping nadh-quinone oxidoreductase with substrates nadh and q2 (see paper)
23% identity, 80% coverage: 51:333/353 of query aligns to 79:405/405 of 8a1uF

query
sites
8a1uF

K

R

S

V

R

K

L

I

L

K

S

S

G

G

E

G

V

G

D

D

L

I

L

L

A

P

H

T

T

E

R

L

F

D

A

H

L

I

T

S

A

K

E

G

E

E

K

A

A

R

Q

E

G

G

L

E

I

R

L

L

A

A

C
|
C

R

Q

A

V

Q

A

P

V

L

K

T

A

D

D

I

M

E

D

V

L

A

E

W

L

L

P

G

E

E

E

A

I

E

-

I

-

E

-

H

F

P

G

V

V

R

K

S

K

L

W

K

E

A

C

E

T

V

V

V

I

A

S

L

N

D

D

S

N

A

K

T

A

H

T

D

F

I

I

N

K

L

E

V

L

R

K

V

L

R

A

V

I

A

P

-

D

G

G

E

E

P

S

L

V

V

P

F

F

T

R

A

A

G

G

Q

G

Y
|
Y

A

I

Q

Q

L

I

S

E

A

A

A

P

G

A

-

H

-

V

-

K

-

Y

-

A

-

D

-

F

-

D

A

V

P

P

-

E

-

K

-

Y

-

R

-

G

-

D

-

W

-

D

-

K

-

F

-

N

-

L

-

F

-

R

-

Y

-

E

-

S

-

K

-

V

-

D

-

E

-

P

-

I

T

I

R
|
R

D
x
A

Y
|
Y

S
|
S

M

M

A

A

N

N

V

Y

P

P

G

E

E

E

E

F

D

G

L

I

E

I

F

M

Y

L

I

N

R

V

H
x
R

I

I

P
x
A

-

T

-

P

-
x
P

-

P

-

N

-

P

-

N

-
x
V

-
x
P

G

P

G
|
G

K
x
Q

A
x
M

S

S

E

S

R

Y

I

I

A

W

T

S

H

-

L

L

A

K

V

A

G

G

D

D

T

K

L

C

T

T

L

I

R

S

G

G

P

P

F

F

G

G

S

E

A

F

Y

F

L

A

R

K

E

D

G

-

H

T

T

D

G

A

P

E

I

M

L

V

A

F

V

I

A

G

G

G

G
|
G

S
x
A

G

G

L

M

A

A

P

P

I

M

K

R

A

S

I

H

V

I

E

F

T

D

A

Q

L

L

E

K

K

R

-

L

G

K

L

S

R

K

Q

R

P

K

I

M

H

S

L

Y

Y

W

F

Y

G
|
G

A

A

R
|
R

R

S

R

K

A

R

D

E

L

M

Y

F

L

Y

V

V

D

E

H

D

F

F

E

D

R

G

L

L

A

A

A

E

H

N

A

D

N

N

F

F

H

V

F

W

E

H

P

C

V

A

L

L

S
|
S

-

D

-

P

R

Q

E

P

S

E

D

D

A

N

G

W

A

T

G

G

R

Y

A

T

G

G

F
|
F

V

I

T

H

D

N

A

V

I

L

A

Y

A

E

D

N

F

Y

T

L

D

K

-

D

-

H

-

E

-

A

L

P

D

E

G

D

W

C

K

E

A

Y

Y

Y

L

M

A
x
C

G
|
G

P
|
P

P

P

A
x
M

M
|
M

I

N

D

A

T

A

A

V

G

I

P

N

L

M

L

L

K

K

A

N

R

L

G

G

L

V

A

E

S

E

E

E

D

N

F

I

H

L

A

L

D
|
D

I

D

F
|
F

binding flavin-adenine dinucleotide: Y166 (= Y141), R209 (= R152), A210 (≠ D153), Y211 (= Y154), S212 (= S155), A230 (≠ P173), P233 (vs. gap), V239 (vs. gap), P240 (vs. gap), G242 (= G175), Q243 (≠ K176), M244 (≠ A177), A282 (≠ S217), F405 (= F333)
binding fe2/s2 (inorganic) cluster: C110 (= C82)
binding 1,4-dihydronicotinamide adenine dinucleotide: S212 (= S155), R228 (≠ H171), G281 (= G216), A282 (≠ S217), G309 (= G243), R311 (= R245), S339 (= S273), F353 (= F285), C377 (≠ A305), G378 (= G306), P379 (= P307), M381 (≠ A309), M382 (= M310), D403 (= D331)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 69, 71, 73, 75, 76, 78

7qu5A X-ray structure of fad domain of nqrf of pseudomonas aeruginosa (see paper)
26% identity, 52% coverage: 152:333/353 of query aligns to 83:278/280 of 7qu5A

query
sites
7qu5A

R
|
R

D
x
A

Y
|
Y

S
|
S

M

M

A

A

N

N

V

Y

P

P

G

E

E

E

E

Q

D

G

L

V

-

V

E

K

F

F

Y
x
N

I

I

R

R

-

I

-
x
A

-
x
S

-

P

-
x
P

-

G

-

S

H

D

I
x
L

P
|
P

G

P

G
|
G

K
x
Q

A
x
M

S
|
S

E

S

R

W

I

V

A

F

T

-

H

N

L

L

A

K

V

P

G

G

D

D

T

K

L

V

T

T

L

V

R

Y

G

G

P

P

F

F

G

G

S

E

A

F

Y

F

L

A

R

K

E

D

G

T

H

E

T

A

G

E

P

-

I

M

L

V

A

F

V

I

A

G

G

G

S
x
A

G

G

L

M

A

A

P

P

I

M

K

R

A

S

I

H

V

I

E

F

T

D

A

Q

L

L

E

R

K

R

-

L

G

K

L

S

R

N

Q

R

P

K

I

I

H

S

L

F

Y

W

F

Y

G

G

A

A

R

R

R

S

R

L

A

R

D

E

L

A

Y

F

L

Y

V

T

D

E

H

E

F

Y

E
x
D

R

Q

L

L

A

Q

A

A

A

E

H

N

A

P

N

N

F

F

H

Q

F
x
W

E
x
H

P
x
L

V

A

L

L

S

S

-

D

-

P

R

Q

E

P

S

E

D

D

A

N

G

W

A

T

G

G

R

L

A

T

G

G

F

F

V

I

T

H

D

N

A

V

I

L

A

F

A

E

D

N

F

Y

T

L

D

K

-

D

-

H

-

P

-

A

L

P

D

E

G

D

W

C

K

E

A

F

Y

Y

L

M

A

C

G

G

P

P

A

M

M

M

I

N

D

A

T

A

A

V

G

I

P

K

L

M

L

L

K

T

A

D

R

L

G

G

L

V

A

E

S

R

E

E

D

N

F

I

H

L

A

L

D

D

I

D

F
|
F

binding flavin-adenine dinucleotide: R83 (= R152), A84 (≠ D153), Y85 (= Y154), S86 (= S155), N100 (≠ Y168), A104 (vs. gap), L112 (≠ I172), P113 (= P173), G115 (= G175), Q116 (≠ K176), M117 (≠ A177), S118 (= S178), A155 (≠ S217), F278 (= F333)
binding gamma-Valerolactone: S105 (vs. gap), P108 (vs. gap), D196 (≠ E257), W207 (≠ F268), H208 (≠ E269), L209 (≠ P270)

Sites not aligning to the query:

7qu3A X-ray structure of fad domain of nqrf of pseudomonas aeruginosa (see paper)
26% identity, 52% coverage: 152:333/353 of query aligns to 83:278/279 of 7qu3A

query
sites
7qu3A

R
|
R

D
x
A

Y
|
Y

S
|
S

M

M

A

A

N

N

V

Y

P

P

G

E

E

E

E

Q

D

G

L

V

-

V

E

K

F

F

Y
x
N

I

I

R
|
R

-

I

-
x
A

-
x
S

-

P

-

G

-

S

H

D

I
x
L

P
|
P

G

P

G
|
G

K
x
Q

A
x
M

S
|
S

E

S

R

W

I

V

A

F

T

-

H

N

L

L

A

K

V

P

G

G

D

D

T

K

L

V

T

T

L

V

R

Y

G

G

P

P

F

F

G

G

S

E

A

F

Y

F

L

A

R

K

E

D

G

T

H

E

T

A

G

E

P

-

I

M

L

V

A

F

V

I

A

G

G

G

G
|
G

S
x
A

G

G

L

M

A

A

P

P

I

M

K

R

A

S

I

H

V

I

E

F

T

D

A

Q

L

L

E

R

K

R

-

L

G

K

L

S

R

N

Q

R

P

K

I

I

H

S

L

F

Y

W

F

Y

G
|
G

A

A

R

R

R

S

R

L

A

R

D

E

L

A

Y

F

L

Y

V

T

D

E

H

E

F

Y

E

D

R

Q

L

L

A

Q

A

A

A

E

H

N

A

P

N

N

F

F

H

Q

F

W

E

H

P

L

V

A

L

L

S

S

-

D

-

P

R

Q

E

P

S

E

D

D

A

N

G

W

A

T

G

G

R

L

A

T

G

G

F
|
F

V
x
I

T

H

D

N

A

V

I

L

A

F

A

E

D

N

F

Y

T

L

D

K

-

D

-

H

-

P

-

A

L

P

D

E

G

D

W

C

K

E

A

F

Y

Y

L

M

A

C

G

G

P
|
P

P

P

A

M

M
|
M

I

N

D

A

T

A

A

V

G

I

P

K

L

M

L

L

K

T

A

D

R

L

G

G

L

V

A

E

S

R

E

E

D

N

F

I

H

L

A

L

D

D

I

D

F
|
F

binding flavin-adenine dinucleotide: R83 (= R152), A84 (≠ D153), Y85 (= Y154), S86 (= S155), N100 (≠ Y168), A104 (vs. gap), S105 (vs. gap), L112 (≠ I172), P113 (= P173), G115 (= G175), Q116 (≠ K176), M117 (≠ A177), S118 (= S178), A155 (≠ S217), F278 (= F333)
binding 4-(benzimidazol-1-ylmethyl)benzenecarbonitrile: R102 (= R170), G154 (= G216), G182 (= G243), F226 (= F285), I227 (≠ V286), P252 (= P307), M255 (= M310)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 40

4u9uA Crystal structure of nqrf fad-binding domain from vibrio cholerae (see paper)
27% identity, 52% coverage: 152:333/353 of query aligns to 81:277/279 of 4u9uA

query
sites
4u9uA

R
|
R

D
x
A

Y
|
Y

S
|
S

M

M

A

A

N

N

V

Y

P

P

G

E

E

E

E

F

D

G

L

I

E

I

F

M

Y

L

I
x
N

R

V

H

R

I

I

-
x
A

-
x
T

-
x
P

-

P

-

N

-

P

-

N

-
x
V

P
|
P

G

P

G
|
G

K
x
Q

A
x
M

S
|
S

E

S

R

Y

I

I

A

W

T

S

H

-

L

L

A

K

V

A

G

G

D

D

T

K

L

C

T

T

L

I

R

S

G

G

P

P

F

F

G

G

S

E

A

F

Y

F

L

A

R

K

E

D

G

T

H

-

T

D

G

A

P

E

I

M

L

V

A

F

V

I

A

G

G

G

S

A

G

G

L

M

A

A

P

P

I

M

K

R

A

S

I

H

V

I

E

F

T

D

A

Q

L

L

E

K

K

R

-

L

G

K

L

S

R

K

Q

R

P

K

I

M

H

S

L

Y

Y

W

F

Y

G

G

A

A

R

R

R

S

R

K

A
x
R

D

E

L
x
M

Y

F

L

Y

V

V

D

E

H

D

F

F

E

D

R

G

L

L

A
|
A

A

A

A

E

H
x
N

A
x
D

N

N

F
|
F

H

V

F

W

E

H

P

C

V

A

L

L

S

S

-

D

-

P

R

Q

E

P

S

E

D

D

A

N

G

W

A

T

G

G

R

Y

A

T

G

G

F

F

V

I

T

H

D

N

A

V

I

L

A

Y

A

E

D

N

F

Y

T

L

D

K

-

D

-

H

-

E

-

A

L

P

D

E

G

D

W

C

K

E

A

Y

Y

Y

L

M

A

C

G

G

P

P

A

M

M

M

I

N

D

A

T

A

A

V

G

I

P

N

L

M

L

L

K

K

A

N

R

L

G

G

L

V

A

E

S

E

E

E

D

N

F

I

H

L

A

L

D

D

I

D

F
|
F

binding flavin-adenine dinucleotide: R81 (= R152), A82 (≠ D153), Y83 (= Y154), S84 (= S155), N98 (≠ I169), A102 (vs. gap), T103 (vs. gap), P104 (vs. gap), V111 (vs. gap), P112 (= P173), G114 (= G175), Q115 (≠ K176), M116 (≠ A177), S117 (= S178), F277 (= F333)
binding magnesium ion: R186 (≠ A248), M188 (≠ L250)
binding sodium ion: A198 (= A260), N201 (≠ H263), D202 (≠ A264), F204 (= F266)

Sites not aligning to the query:

7romA Crystal structure of saccharomyces cerevisiae nadh-cytochrome b5 reductase 1 (cbr1) fragment (residues 28-284) bound to fad
25% identity, 46% coverage: 151:311/353 of query aligns to 60:227/255 of 7romA

query
sites
7romA

T

T

R
|
R

D
x
S

Y
|
Y

S
x
T

M

P

A

T

N

S

V

L

P

D

G

G

E

D

E

T

-

K

-

G

D

N

L

F

E

E

F

L

Y
x
L

I
x
V

R

K

H

S

I
x
Y

P

P

G
x
T

G
|
G

K
x
N

A
x
V

S
|
S

E

K

R

M

I

I

A

G

T

-

H

E

L

L

A

K

V

I

G

G

D

D

T

S

L

I

T

Q

L

I

R

K

G

G

P

P

F

R

G

G

S

N

A

Y

Y

H

L

Y

R

E

E

R

G

N

H

C

T

R

G

S

P

H

I

L

L

G

A

M

V

I

A

A

G

G

S
x
T

G

G

L

I

A
|
A

P

P

I

M

K

Y

A

Q

I

I

V

M

E

K

-

A

T

I

A

A

L

M

E

D

K

P

G

H

L

D

R

T

Q

T

P

K

I

V

H

S

L

L

Y

V

F

F

G

G

A

N

R

V

R

H

R

E

A

E

D

D

L

I

Y

L

L

L

V

K

D

K

H

E

F

L

E

E

R

A

L

L

A

V

A

A

A

M

H

K

A

P

N

S

F

-

H

Q

F

F

E

K

P

I

V

V

L

Y

S

Y

R

L

E

D

S

S

-

P

-

D

-

R

D

E

A

D

G

W

A

T

G

G

R

G

A

V

G

G

F

Y

V

I

T

T

D

K

A

D

I

V

A

I

A

K

D

E

F

H

-

L

-

P

-

A

T

A

D

T

L

M

D

D

G

N

W

V

K

Q

A

I

Y

L

L

I

A

C

G

G

P

P

A

A

M

M

I

V

binding flavin-adenine dinucleotide: R61 (= R152), S62 (≠ D153), Y63 (= Y154), T64 (≠ S155), L79 (≠ Y168), V80 (≠ I169), Y83 (≠ I172), T85 (≠ G174), G86 (= G175), N87 (≠ K176), V88 (≠ A177), S89 (= S178), T127 (≠ S217), A130 (= A220)

3ozwA The crystal structure of flavohemoglobin from r. Eutrophus in complex with ketoconazole (see paper)
25% identity, 58% coverage: 131:333/353 of query aligns to 179:396/403 of 3ozwA

query
sites
3ozwA

G

G

E

G

P

P

L

V

V

V

-

N

F

F

T

E

A

P

G

G

Q

Q

Y

Y

A

T

Q

S

L

V

S

-

A

A

A

I

G

D

A

V

P

P

-

A

-

L

-

G

-

L

-

Q

T

I

R
|
R

D
x
Q

Y
|
Y

S
|
S

M

L

A

S

N

D

V

M

P

P

G

N

E

G

E

R

D

S

L

Y

E

R

F

I

Y
x
S

I
x
V

R
x
K

H

R

-
x
E

-

G

-

P

I

Q

P
|
P

G

P

G
|
G

K
x
Y

A
x
V

S
|
S

E

N

R

L

I

L

A

H

T

D

H

H

L

V

A

N

V

V

G

G

D

D

T

Q

L

V

T

K

L

L

R

A

G

A

P

P

F

Y

G

G

S

S

A

F

Y

H

L

I

R

D

E

V

G

D

H

A

T

K

G

T

P

P

I

I

L

V

A

L

V

I

A

S

G

G

S
x
V

G

G

L

L

A
x
T

P

P

I

M

K

V

A

S

I

M

V

L

E

K

T

V

A

A

L

L

E

Q

K

A

G

P

L

P

R

R

Q

Q

P

V

I

V

H

F

L

V

Y

H

F

-

G

G

A

A

R

R

R

N

R

S

A

A

D

V

L

H

Y

A

L

M

V

R

D

D

H

R

F

L

E

R

R

E

L

A

A

A

A

K

A

T

H

Y

A

E

N

N

-

L

-

D

-

L

-

F

-

V

F

F

H

Y

F

D

E

Q

P

P

V

L

L

P

S

E

R

D

E

V

S

Q

D

G

A

R

G

D

A

Y

G

D

R

Y

A

P

G

G

F

L

V

V

T

D

D

V

A

K

I

Q

A

I

A

E

D

K

F

S

T

I

D

L

L

L

D

P

G

D

W

A

K

D

A

Y

Y

Y

L

I

A

C

G

G

P

P

P

I

A

P

M

F

I

M

D

R

T

M

A

Q

G

H

P

D

L

A

L

L

K

K

A

N

R

L

G

G

L

I

A

H

S

E

E

A

D

R

F

I

H

H

A

Y

D

E

I
x
V

F
|
F

binding flavin-adenine dinucleotide: R206 (= R152), Q207 (≠ D153), Y208 (= Y154), S209 (= S155), S222 (≠ Y168), V223 (≠ I169), K224 (≠ R170), E226 (vs. gap), P232 (= P173), G234 (= G175), Y235 (≠ K176), V236 (≠ A177), S237 (= S178), V276 (≠ S217), T279 (≠ A220), V395 (≠ I332), F396 (= F333)

Sites not aligning to the query:

3ozvA The crystal structure of flavohemoglobin from r. Eutrophus in complex with econazole (see paper)
25% identity, 58% coverage: 131:333/353 of query aligns to 179:396/403 of 3ozvA

query
sites
3ozvA

G

G

E

G

P

P

L

V

V

V

-

N

F

F

T

E

A

P

G

G

Q

Q

Y

Y

A

T

Q

S

L

V

S

-

A

A

A

I

G

D

A

V

P

P

-

A

-

L

-

G

-

L

-

Q

T

I

R
|
R

D
x
Q

Y
|
Y

S
|
S

M

L

A

S

N

D

V

M

P

P

G

N

E

G

E

R

D

S

L

Y

E

R

F

I

Y
x
S

I
x
V

R
x
K

H

R

-
x
E

-

G

-

P

I

Q

P
|
P

G

P

G
|
G

K
x
Y

A
x
V

S
|
S

E

N

R

L

I

L

A

H

T

D

H

H

L

V

A

N

V

V

G

G

D

D

T

Q

L

V

T

K

L

L

R

A

G

A

P

P

F

Y

G

G

S

S

A

F

Y

H

L

I

R

D

E

V

G

D

H

A

T

K

G

T

P

P

I

I

L

V

A

L

V

I

A

S

G

G

S
x
V

G

G

L

L

A
x
T

P

P

I

M

K

V

A

S

I

M

V

L

E

K

T

V

A

A

L

L

E

Q

K

A

G

P

L

P

R

R

Q

Q

P

V

I

V

H

F

L

V

Y

H

F

-

G

G

A

A

R

R

R

N

R

S

A

A

D

V

L

H

Y

A

L

M

V

R

D

D

H

R

F

L

E

R

R

E

L

A

A

A

A

K

A

T

H

Y

A

E

N

N

-

L

-

D

-

L

-

F

-

V

F

F

H

Y

F

D

E

Q

P

P

V

L

L

P

S

E

R

D

E

V

S

Q

D

G

A

R

G

D

A

Y

G

D

R

Y

A

P

G

G

F

L

V

V

T

D

D

V

A

K

I

Q

A

I

A

E

D

K

F

S

T

I

D

L

L

L

D

P

G

D

W

A

K

D

A

Y

Y

Y

L

I

A

C

G

G

P

P

P

I

A

P

M

F

I

M

D

R

T

M

A

Q

G

H

P

D

L

A

L

L

K

K

A

N

R

L

G

G

L

I

A

H

S

E

E

A

D

R

F

I

H

H

A

Y

D

E

I

V

F
|
F

binding flavin-adenine dinucleotide: R206 (= R152), Q207 (≠ D153), Y208 (= Y154), S209 (= S155), S222 (≠ Y168), V223 (≠ I169), K224 (≠ R170), E226 (vs. gap), P232 (= P173), G234 (= G175), Y235 (≠ K176), V236 (≠ A177), S237 (= S178), V276 (≠ S217), T279 (≠ A220), F396 (= F333)

Sites not aligning to the query:

binding 1-[(2s)-2-[(4-chlorobenzyl)oxy]-2-(2,4-dichlorophenyl)ethyl]-1h-imidazole: 24, 28, 57, 102, 106
binding flavin-adenine dinucleotide: 44, 46, 48
binding protoporphyrin ix containing fe: 43, 81, 84, 85, 88, 90, 94, 95, 98, 126, 130, 133

Query Sequence

>Ga0059261_0425 FitnessBrowser__Korea:Ga0059261_0425
VNGIVVTRTVHIRQTGRPIAVGDKQTILAAALDAGVAFPHGCRSGRCGACKSRLLSGEVD
LLAHTRFALTAEEKAQGLILACRAQPLTDIEVAWLGEAEIIEHPVRSLKAEVVALDSATH
DINLVRVRVAGEPLVFTAGQYAQLSAAGAPTRDYSMANVPGEEDLEFYIRHIPGGKASER
IATHLAVGDTLTLRGPFGSAYLREGHTGPILAVAGGSGLAPIKAIVETALEKGLRQPIHL
YFGARRRADLYLVDHFERLAAAHANFHFEPVLSRESDAGAGRAGFVTDAIAADFTDLDGW
KAYLAGPPAMIDTAGPLLKARGLASEDFHADIFFTPEPPLREDLRQTTEGAAS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory