SitesBLAST
Comparing Ga0059261_0617 FitnessBrowser__Korea:Ga0059261_0617 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2nwwA Crystal structure of gltph in complex with tboa (see paper)
30% identity, 83% coverage: 47:415/445 of query aligns to 34:407/407 of 2nwwA
O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
30% identity, 89% coverage: 25:419/445 of query aligns to 15:420/425 of O59010
- S65 (≠ T69) mutation to V: Strongly decreased chloride conductance.
- R276 (≠ S280) mutation to S: Increased rate of aspartate transport; when associated with R-395.
- RSS 276:278 (≠ SSS 280:282) binding
- M311 (≠ L315) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
- T314 (= T318) binding
- V355 (= V359) binding
- D394 (≠ S393) binding
- M395 (≠ E394) mutation to R: Increased rate of aspartate transport; when associated with S-276.
- R397 (= R396) mutation to A: Strongly decreased affinity for aspartate.
- N401 (= N400) binding
- D405 (≠ N404) mutation to N: Strongly decreased affinity for aspartate.
6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
30% identity, 84% coverage: 47:418/445 of query aligns to 43:419/419 of 6x15A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (≠ L50), F46 (≠ L50), P75 (≠ E79), L91 (≠ F96), F95 (≠ L100), L130 (≠ I142), I133 (≠ F145), I159 (= I171), Y167 (≠ M179), K196 (= K200), G200 (≠ I204), I207 (≠ V211), F210 (= F214), L250 (≠ V254), I262 (= I266), M269 (≠ L273), T334 (≠ S338), V335 (≠ L339), G336 (= G340), T340 (≠ A344), L343 (≠ A347), M399 (≠ L398)
- binding aspartic acid: S277 (= S281), S278 (= S282), T314 (= T318), G354 (= G358), A358 (= A362), G359 (= G363), D394 (≠ S393), R397 (= R396), T398 (≠ S397)
- binding sodium ion: Y89 (≠ F94), T92 (≠ F97), S93 (= S98), G306 (= G310), T308 (≠ S312), N310 (= N314), N310 (= N314), M311 (≠ L315), D312 (= D316), S349 (= S353), I350 (≠ K354), T352 (≠ A356), N401 (= N400), V402 (≠ F401), D405 (≠ N404)
Sites not aligning to the query:
6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
32% identity, 84% coverage: 47:421/445 of query aligns to 39:420/425 of 6zgbA
6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
30% identity, 83% coverage: 47:415/445 of query aligns to 40:413/413 of 6x14A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G73), V83 (≠ F91), I157 (≠ L172), Y164 (≠ M179), K193 (= K200), T305 (≠ S312), I306 (≠ F313), I347 (≠ K354)
- binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: M199 (= M206), S275 (= S282), T311 (= T318), G356 (= G363), L384 (= L386), D391 (≠ S393), R394 (= R396)
Sites not aligning to the query:
6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
32% identity, 84% coverage: 47:421/445 of query aligns to 38:419/424 of 6zl4A
- binding decyl-beta-d-maltopyranoside: L191 (vs. gap), G195 (≠ I204), R282 (≠ D291)
- binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ S280), S272 (= S281), S273 (= S282), M307 (≠ L315), T310 (= T318), G353 (= G361), A354 (= A362), R394 (= R396), T395 (≠ S397)
Sites not aligning to the query:
5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
32% identity, 84% coverage: 47:421/445 of query aligns to 41:422/427 of 5e9sA
- binding aspartic acid: R274 (≠ S280), S275 (= S281), S276 (= S282), T313 (= T318), G353 (= G358), V354 (= V359), A357 (= A362), G358 (= G363), D394 (≠ S393), R397 (= R396), T398 (≠ S397)
- binding decyl-beta-d-maltopyranoside: L194 (vs. gap), G198 (≠ I204), Y202 (≠ A208)
- binding sodium ion: Y87 (≠ F94), T90 (≠ F97), S91 (= S98), S276 (= S282), G305 (= G310), A306 (≠ Y311), T307 (≠ S312), N309 (= N314), N309 (= N314), M310 (≠ L315), D311 (= D316), S348 (= S353), I349 (≠ K354), G350 (= G355), T351 (≠ A356), N401 (= N400), V402 (≠ F401), D405 (≠ N404)
6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
32% identity, 84% coverage: 47:421/445 of query aligns to 41:422/426 of 6xwnB
6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
31% identity, 84% coverage: 47:421/445 of query aligns to 34:411/416 of 6r7rA
- binding d-aspartic acid: R263 (≠ S280), S265 (= S282), M299 (≠ L315), T302 (= T318), T340 (≠ A356), G342 (= G358), V343 (= V359), G347 (= G363), D383 (≠ S393), R386 (= R396), T387 (≠ S397), N390 (= N400)
- binding decyl-beta-d-maltopyranoside: V212 (≠ L229), A216 (= A233)
Sites not aligning to the query:
6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
30% identity, 88% coverage: 25:415/445 of query aligns to 7:408/408 of 6bauA
- binding cysteine: S270 (= S282), M303 (≠ L315), T306 (= T318), A345 (= A357), G346 (= G358), V347 (= V359), G351 (= G363), D386 (≠ S393), C389 (≠ R396), T390 (≠ S397), N393 (= N400)
6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
30% identity, 88% coverage: 25:415/445 of query aligns to 7:408/409 of 6bavA
6bmiA Crystal structure of gltph r397c in complex with l-serine (see paper)
28% identity, 88% coverage: 25:415/445 of query aligns to 7:396/396 of 6bmiA
7awmA Structure of the thermostabilized eaat1 cryst mutant in complex with l-asp, three sodium ions and the allosteric inhibitor ucph101 (see paper)
29% identity, 80% coverage: 51:406/445 of query aligns to 57:393/412 of 7awmA
- binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: S88 (≠ V83), G89 (= G84), G92 (≠ A87), A95 (= A90), V96 (≠ F91), Y99 (≠ F94), M163 (≠ I171), F167 (≠ I175), F293 (≠ L305), V297 (≠ T309)
- binding aspartic acid: S268 (= S281), S269 (= S282), T306 (= T318), G346 (= G358), I347 (≠ V359), A350 (= A362), G351 (= G363), D380 (≠ S393), R383 (= R396), T384 (≠ S397)
5mjuA Structure of the thermostabilized eaat1 cryst mutant in complex with the competititve inhibitor tfb-tboa and the allosteric inhibitor ucph101 (see paper)
28% identity, 80% coverage: 51:406/445 of query aligns to 49:379/397 of 5mjuA
- binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: L72 (≠ I74), S80 (≠ V83), G81 (= G84), G84 (≠ A87), Y91 (≠ F94), M156 (≠ I171), F160 (≠ I175), F286 (≠ L305), V290 (≠ T309)
- binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I64 (= I66), I148 (≠ L163), S262 (= S282), S263 (≠ E283), A292 (≠ Y311), T293 (≠ S312), M296 (≠ L315), T299 (= T318), G329 (= G355), A336 (= A362), G337 (= G363), D366 (≠ S393), R369 (= R396), N373 (= N400)
Q10901 Excitatory amino acid transporter; Sodium-dependent glutamate/ aspartate transporter from Caenorhabditis elegans (see paper)
27% identity, 82% coverage: 51:417/445 of query aligns to 56:462/503 of Q10901
- N177 (≠ H138) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- N187 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
7xr4A Structure of human excitatory amino acid transporter 2 (eaat2) in complex with glutamate (see paper)
29% identity, 83% coverage: 51:420/445 of query aligns to 46:421/425 of 7xr4A
7xr6A Structure of human excitatory amino acid transporter 2 (eaat2) in complex with way-213613 (see paper)
29% identity, 83% coverage: 51:420/445 of query aligns to 47:420/424 of 7xr6A
- binding (2S)-2-azanyl-4-[[4-[2-bromanyl-4,5-bis(fluoranyl)phenoxy]phenyl]amino]-4-oxidanylidene-butanoic acid: S280 (= S281), S281 (= S282), T318 (= T318), G363 (= G363), M367 (≠ L367), V385 (≠ L386), D388 (= D389), R395 (= R396), T396 (≠ S397)
- binding dodecyl beta-D-glucopyranoside: W389 (≠ R390)
- binding cholesterol hemisuccinate: R80 (= R85), R84 (≠ K89), I95 (≠ L100), I252 (≠ V250)
Sites not aligning to the query:
P43003 Excitatory amino acid transporter 1; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3 from Homo sapiens (Human) (see 3 papers)
25% identity, 82% coverage: 51:413/445 of query aligns to 85:496/542 of P43003
- S363 (= S280) mutation to R: Loss of electrogenic glutamate transport. Strongly decreased L-aspartate and L-glutamate uptake combined with strongly increased permeability ot other ions; when associated with M-477.
- SSS 363:365 (= SSS 280:282) binding
- T396 (≠ S312) binding
- T402 (= T318) binding
- IPQAG 443:447 (≠ VTGAG 359:363) binding
- D476 (≠ S393) binding
- R477 (≠ E394) mutation to M: Strongly decreased L-aspartate and L-glutamate uptake combined with strongly increased permeability ot other ions; when associated with R-363.
- N483 (= N400) binding
Sites not aligning to the query:
- 523 Y→F: No effect on activity.
7vr7A Inward-facing structure of human eaat2 in the way213613-bound state (see paper)
28% identity, 80% coverage: 51:405/445 of query aligns to 39:389/402 of 7vr7A
- binding (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en: S57 (≠ T69), L58 (= L70), L65 (≠ M77), V339 (≠ K354), G340 (= G355), S343 (≠ G358), I344 (≠ V359)
- binding cholesterol: W188 (≠ R207), I227 (vs. gap), F250 (= F263), W257 (≠ L273), M379 (≠ C395), S382 (≠ L398)
- binding (2S)-2-azanyl-4-[[4-[2-bromanyl-4,5-bis(fluoranyl)phenoxy]phenyl]amino]-4-oxidanylidene-butanoic acid: S266 (= S282), M300 (≠ L315), T303 (= T318), Y306 (= Y321), G348 (= G363), L349 (≠ F364), M352 (≠ L367), I366 (≠ M382), L369 (≠ I385), V370 (≠ L386), D373 (= D389), D377 (≠ S393), R380 (= R396), T381 (≠ S397), N384 (= N400)
Sites not aligning to the query:
P56564 Excitatory amino acid transporter 1; Glial high affinity glutamate transporter; High-affinity neuronal glutamate transporter; GluT-1; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3 from Mus musculus (Mouse) (see paper)
25% identity, 82% coverage: 51:413/445 of query aligns to 85:496/543 of P56564
- N206 (≠ D139) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- N216 (≠ I149) modified: carbohydrate, N-linked (GlcNAc...) asparagine
Query Sequence
>Ga0059261_0617 FitnessBrowser__Korea:Ga0059261_0617
MAIALDYASEPQRRRGAWYGQLYVQVLVAIAAGVSVGYFWPDAGASLKPLGDAFIKLVKM
IIAPVIFLTLVTGIAGMTELKSVGRVAGKAFAYFLFFSTLALVVGLIVANTVQPGAGMNV
DPASLDTGAVKDYVAKAHDSSIVGFLMAIIPTTLVSALTGESLLQVLLVSILFGIALSMV
GEPAAPVRDLLEKTGLVVFKLVGILMRAAPVGAFGAMAFTIGKYGIESLANLAGLVATFY
LTSAIFVVVVLGVVAKLAGFSIFKLIRYLRAELLLVLGTSSSESALPSLMDKMERAGCAK
PVVGLVVPTGYSFNLDGTNIYMTLAALFIAQACNVDLSLGDQIALLAIAMISSKGAAGVT
GAGFITLAATLSIVPDVPVAGMALILGIDRFMSECRSLTNFIGNAVATIVVARWDNALDT
AALHHALNAPTAPGAARPATESDMD
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory