SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ga0059261_0833 FitnessBrowser__Korea:Ga0059261_0833 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O86033 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
48% identity, 100% coverage: 1:490/491 of query aligns to 1:494/497 of O86033

query
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O86033

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R82 (= R81) binding glycerol
E83 (= E82) binding glycerol
Y134 (= Y133) binding glycerol
D243 (= D242) binding glycerol
Q244 (= Q243) binding glycerol

P18157 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Bacillus subtilis (strain 168) (see paper)
44% identity, 100% coverage: 1:491/491 of query aligns to 1:494/496 of P18157

query
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P18157

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H230 (vs. gap) mutation to R: Increased activity.
F232 (= F230) mutation to S: Increased activity.

3ge1A 2.7 angstrom crystal structure of glycerol kinase (glpk) from staphylococcus aureus in complex with adp and glycerol
46% identity, 99% coverage: 1:487/491 of query aligns to 2:491/499 of 3ge1A

query
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3ge1A

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S

V

A

V
x
I

K
x
Q

W

W

L

L

R

R

D

D

S

G

L

L

G

R

L

M

I

I

E

N

S

S

A
|
A

G
x
P

E

Q

T

S

E

E

V

S

L

Y

A

A

R

T

S

R

V

V

P

D

D

S

N

T

G

E

G

G

V

V

Y

Y

F

V

V

V

P

P

A

A

L

F

T

V

G

G

L

L

G

G

A

T

P

P

W

Y

W

W

E

D

P

S

E

E

A

A

R

R

G

G

A

A

I

I

S

F

G

G

L

L

S

T

L

R

A

G

S

T

T

E

R

K

A

E

H

H

V

F

V

I

R

R

A

A

A

T

L

L

E

E

A

S

M

L

A

C

H

Y

Q

Q

S

T

H

R

D

D

L

V

K

M

A

E

A

A

F

M

A

S

A

K

D

D

-

S

G

G

A

I

D

D

W

V

S

Q

S

S

L

L

R

R

I

V

D

D

G

G

G
|
G

M
x
A

I

V

T

K

N
|
N

D

N

W

F

I

I

A

M

Q

Q

D

F

L

Q

A

A

D

D

V

I

L

V

D

N

L

T

T

S

V

V

E

E

R

R

P

P

D

E

F

I

T

Q

E

E

T

T

A

A

L

L

G

G

A

A

M

F

L

L

A

A

G

G

V

L

G

A

Y

V

G

G

L

F

F

W

A

E

G

S

L

K

D

D

E

D

A

I

A

A

A

K

M

N

R

W

G

K

K

L

V

E

E

E

R

K

F

F

A

D

P

P

G

K

M

M

K

D

V

E

E

G

A

E

R

R

D

E

A

K

R

L

L

Y

A

R

G

G

W

W

K

K

A

K

A

A

V

V

E

E

binding adenosine-5'-diphosphate: G266 (= G263), T267 (= T264), G310 (= G307), I313 (≠ V310), Q314 (≠ K311), A326 (= A323), P327 (≠ G324), G411 (= G407), A412 (≠ M408), N415 (= N411)

Q5HGD2 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Staphylococcus aureus (strain COL)
46% identity, 99% coverage: 1:487/491 of query aligns to 1:490/498 of Q5HGD2

query
sites
Q5HGD2

M

M

G

E

E

K

K

Y

L

I

L

L

V

S

I

I

D

D

E

Q

G

G

T
|
T

T

T

S

S

T

S

R
|
R

A

A

M

I

L

L

F

F

A

N

P

Q

D

K

G

G

T

E

L

I

L

A

A

G

S

V

R

A

S

Q

A

R

E

E

L

F

A

K

Q

Q

H

Y

Y

F

P

P

A

Q

P

S

G

G

L

W

V

V

E

E

H

H

D

D

A

A

A

N

E

E

I

I

W

W

E

T

K

S

T

V

L

L

G

A

C

V

A

M

V

T

A

E

M

V

V

I

-

N

E

E

A

N

A

D

G

V

G

R

A

A

D

D

R

Q

I

I

A

A

A

G

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

I

T

V

V

F

V

W

W

D

D

K

K

A

H

T

T

G

G

E

R

P

P

L

I

A

Y

P

H

A

A

I

I

V

V

W

W

Q

Q

D

S

R

R

R

Q

S

T

A

Q

A

S

I

I

C

C

R

S

E

E

L

L

K

K

E

Q

A

Q

G

G

E

Y

E

E

P

Q

G

T

V

F

Q

R

A

D

R

K

T

T

G

G

L

L

D

D

P

P

Y
|
Y

F

F

S

A

G

G

T

T

K

K

I

V

A

K

W

W

A

I

M

L

A

D

N

N

W

V

P

E

Q

G

L

A

R

R

E

E

A

K

G

A

D

E

R

N

-

G

-

D

L

L

A

L

I

F

G

G

T

T

V

I

E

D

S

T

W

W

L

L

V

V

W

W

K

K

L

L

T

S

A

G

N

K

E

-

K

-

R

-

G

-

G

A

L

A

H

H

I

I

T

T

D

D

A

Y

T

S

N

N

A

A

S

S

R

R

T

T

L

L

L

M

M

F

G

N

L

I

G

H

S

D

G

L

G

E

W

W

S

D

D

D

G

E

L

L

I

L

D

E

L

L

F

L

G

T

A

V

P

P

R

K

A

N

A

M

L

L

A

P

E

E

I

V

V

K

D

A

C

S

A

S

G

E

R

V

F

Y

G

G

E

K

T

T

T

I

-

D

-

Y

-

H

L

F

F

Y

G

G

G

Q

P

E

I

V

P

P

I

I

C

A

G

G

M

V

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

T

L

I

F

G

G

Q

Q

A

A

C

C

L

F

S

E

P

R

G

G

D

D

T

V

K

K

A

N

T

T

F

Y

G

G

T
|
T

G

G

A

G

F

F

V

M

L

L

T

M

N

N

A

T

G

G

E

D

R

K

Q

A

P

V

A

K

S

S

K

E

H

S

R

G

L

L

S

T

T

T

V

I

L

A

W

Y

Q

G

L

I

G

D

G

G

R

K

R

V

T

N

Y

Y

A

A

I

L

E

E

G

G

S

S

V

I

F

F

V

V

A

S

G
|
G

S

S

V

A

V

I

K
x
Q

W

W

L

L

R

R

D

D

S

G

L

L

G

R

L

M

I

I

E

N

S

S

A

A

G

P

E

Q

T

S

E

E

V

S

L

Y

A

A

R

T

S

R

V

V

P

D

D

S

N

T

G

E

G

G

V

V

Y

Y

F

V

V

V

P

P

A

A

L

F

T

V

G

G

L

L

G

G

A

T

P

P

W

Y

W

W

E

D

P

S

E

E

A

A

R

R

G

G

A

A

I

I

S

F

G

G

L

L

S

T

L

R

A

G

S

T

T

E

R

K

A

E

H

H

V

F

V

I

R

R

A

A

A

T

L

L

E

E

A

S

M

L

A

C

H

Y

Q

Q

S

T

H

R

D

D

L

V

K

M

A

E

A

A

F

M

A

S

A

K

D

D

-

S

G

G

A

I

D

D

W

V

S

Q

S

S

L

L

R

R

I

V

D

D

G

G

G
|
G

M

A

I

V

T

K

N
|
N

D

N

W

F

I

I

A

M

Q

Q

D

F

L

Q

A

A

D

D

V

I

L

V

D

N

L

T

T

S

V

V

E

E

R

R

P

P

D

E

F

I

T

Q

E

E

T

T

A

A

L

L

G

G

A

A

M

F

L

L

A

A

G

G

V

L

G

A

Y

V

G

G

L

F

F

W

A

E

G

S

L

K

D

D

E

D

A

I

A

A

A

K

M

N

R

W

G

K

K

L

V

E

E

E

R

K

F

F

A

D

P

P

G

K

M

M

K

D

V

E

E

G

A

E

R

R

D

E

A

K

R

L

L

Y

A

R

G

G

W

W

K

K

A

K

A

A

V

V

E

E

T12 (= T12) binding ADP
R16 (= R16) binding ADP
R82 (= R81) binding glycerol
E83 (= E82) binding glycerol
Y134 (= Y133) binding glycerol
D244 (= D242) binding glycerol
Q245 (= Q243) binding glycerol
T266 (= T264) binding ADP
G309 (= G307) binding ADP
Q313 (≠ K311) binding ADP
G410 (= G407) binding ADP
N414 (= N411) binding ADP

6udeB Crystal structure of glycerol kinase from elizabethkingia anophelis nuhp1 in complex with adp and glycerol
46% identity, 99% coverage: 2:487/491 of query aligns to 1:487/495 of 6udeB

query
sites
6udeB

G

N

E

E

K

K

L

L

-

I

L

L

V

A

I

L

D

D

E

Q

G
|
G

T
|
T

S
|
S

T

S

R
|
R

A

A

M

I

L

L

F

F

A

N

P

K

D

S

G

G

T

E

L

I

L

K

A

F

S

V

R

S

S

Q

A

K

E

S

L

F

A

E

Q

Q

H

I

Y

F

P

P

A

T

P

P

G

G

L

W

V

V

E

E

H

H

D

D

A

P

A

N

E

E

I

I

W

W

E

S

K

S

T

Q

L

I

G

S

C

V

A

A

V

A

A

E

M

V

V

I

E

A

A

K

A

A

G

G

G

I

A

S

D

G

-

L

R

E

I

V

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R

R

E

E

T

T

I

T

V

V

F

V

W

W

D

D

K

R

A

H

T

T

G

S

E

E

P

P

L

I

A

Y

P

N

A

A

I

I

V

V

W

W

Q

Q

D

D

R

R

S

T

A

S

A

K

I

Y

C

C

R

D

E

E

L

L

K

K

E

S

A

Q

G

G

E

H

E

T

P

D

G

E

V

I

Q

K

A

Q

R

K

T

T

G

G

L

L

L

V

L

L

D

D

P

A

Y

Y

F

F

S

S

G

A

T

T

K

K

I

L

A

K

W

W

A

I

M

L

A

D

N

N

W

V

P

E

Q

G

L

A

R

R

E

E

-

K

-

A

-

E

A

A

G

G

D

D

R

-

L

L

A

C

I

F

G

G

T

T

V

V

E

D

S

T

W

W

L

L

V

I

W

W

K

K

L

L

T

T

A

-

N

-

E

-

K

-

R

R

G

G

G

K

L

M

H

F

I

I

T

T

D

D

A

V

T

S

N

N

A

A

S

S

R

R

T

T

L

M

M

F

G

N

L

I

G

R

S

T

G

M

G

D

W

W

S

D

D

D

G

D

L

L

I

L

D

K

L

L

F

F

G

N

A

I

P

P

R

R

A

A

A

I

L

L

A

P

E

E

I

V

V

K

D

Q

C

S

A

S

G

E

R

V

F

Y

G

G

E

E

T

T

T

S

L

T

F

T

G

S

G

T

P

K

I

I

P

P

I

I

C

A

G

G

M

I

A

A

G

G

D

D

Q

Q

A

A

T

L

I

F

G

G

Q

Q

A

M

C

C

L

T

S

K

P

P

G

G

D

M

T

V

K

K

A

N

T

T

F

Y

G
|
G

T
|
T

G

G

A

C

F

F

V

L

L

L

T

M

N

N

A

T

G

G

E

N

R

E

Q

A

P

V

A

Y

S

S

K

K

H

N

R

N

L

L

S

T

T

T

V

V

L

A

W

W

Q

K

L

I

G

N

G

G

R

E

R

V

T

S

Y

Y

A

A

I

L

E

E

G

G

S

S

V

V

F

F

V

V

A

G

G
|
G

S

A

V

A

V
x
I

K

Q

W

W

L

L

R

R

D

D

S

G

L

L

G

K

L

I

I

I

E

H

S

D

A

S

G
x
S

E

E

T

V

E

S

V

T

L

L

A

A

R

E

S

T

V

V

P

E

D

D

N

N

G

G

V

V

Y

Y

F

F

V

V

P

P

A

A

L

L

T

T

G

G

L

L

G

G

A

A

P

P

W

Y

W

W

E

D

P

Q

E

Y

A

A

R

R

G

G

A

T

I

I

S

I

G

G

L

V

S

T

L

R

A

G

S

T

T

T

R

D

A

G

H

H

V

I

V

A

R

R

A

A

A

T

L

L

E

E

A

G

M

I

A

A

H

F

Q

Q

S

V

H

Y

D

D

L

I

K

V

A

K

A

A

F

M

A

E

A

A

D

D

G

A

A

E

D

T

W

Q

S

S

S

T

-

E

L

L

R

R

I

V

D

D

G
|
G

M
x
A

I

S

T

A

N

S

D

N

W

L

I

L

A

M

Q

Q

D

I

L

Q

A

S

D

D

V

L

L

F

D

G

L

F

T

K

V

I

E

I

R

R

P

P

D

K

F

T

T

L

E

E

T

T

A

A

L

L

G

G

A

A

M

Y

L

L

A

A

G

G

V

L

G

A

Y

V

G

G

L

F

F

W

A

E

G

S

L

I

D

D

E

E

A

I

A

Q

A

S

M

Q

R

W

G

I

K

I

V

E

E

K

R

E

F

F

A

T

P

P

G

K

M

E

K

D

V

K

E

T

A

K

R

I

D

D

A

N

R

M

L

V

A

S

G

F

W

W

K

H

A

K

A

A

V

V

E

K

binding adenosine-5'-diphosphate: R16 (= R16), G262 (= G263), T263 (= T264), G306 (= G307), I309 (≠ V310), S323 (≠ G324), G406 (= G406), G407 (= G407), A408 (≠ M408)
binding magnesium ion: G11 (= G11), T12 (= T12), T13 (= T13), S14 (= S14)

1gllO Escherichia coli glycerol kinase mutant with bound atp analog showing substantial domain motion (see paper)
45% identity, 99% coverage: 5:491/491 of query aligns to 5:490/494 of 1gllO

query
sites
1gllO

L

I

L

V

V

A

I

L

D

D

E

Q

G

G

T
|
T

S

S

T

S

R

R

A

A

M

V

L

V

F

M

A

D

P

H

D

D

G

A

T

N

L

I

A

S

S

V

R

S

S

Q

A

R

E

E

L

F

A

E

Q

Q

H

I

Y

Y

P

P

A

K

P

P

G

G

L

W

V

V

E

E

H

H

D

D

A

P

A

M

E

E

I

I

W

W

-

A

E

T

K

Q

T

S

L

W

G

T

C

L

A

V

V

E

A

V

M

L

V

A

E

K

A

A

A

D

G

I

G

S

A

S

D

D

R

Q

I

I

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

I

T

V

I

F

V

W

W

D

E

K

K

A

E

T

T

G

G

E

K

P

P

L

I

A

Y

P

N

A

A

I

I

V

V

W

W

Q

Q

D

C

R

R

S

T

A

A

A

E

I

I

C

C

R

E

E

H

L

L

K

K

E

R

A

D

G

G

E

L

E

E

P

D

G

Y

V

I

Q

R

A

S

R

N

T

T

G

G

L

L

L

V

L

I

D

D

P

P

Y
|
Y

F

F

S

S

G

G

T

T

K

K

I

V

A

K

W

W

A

I

M

L

A

D

N

H

W

V

P

E

Q

G

L

S

R

R

E

E

A

R

G

A

D

R

R

R

-

G

-

E

L

L

A

L

I

F

G

G

T

T

V

V

E

D

S

T

W

W

L

L

V

I

W

W

K

K

L

M

T

T

A

-

N

-

E

-

K

-

R

Q

G

G

G

R

L

V

H

H

I

V

T

T

D

D

A

Y

T

T

N

N

A

A

S

S

R

R

T

T

L

M

L

L

M

F

G

N

L

I

G

H

S

T

G

L

G

D

W

W

S

D

D

D

G

K

L

M

I

L

D

E

L

V

F

L

G

D

A

I

P

P

R

R

A

E

A

M

L

L

A

P

E

E

I

V

V

R

D

R

C

S

A

S

G

E

R

V

F

Y

G

G

E

Q

T

T

T

N

L

I

F

-

G

G

G

T

P

R

I

I

P

P

I

I

C

S

G

G

M

I

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

T

L

I

F

G

G

Q

Q

A

L

C

C

L

V

S

K

P

E

G

G

D

M

T

A

K

K

A

N

T

T

F

Y

G
|
G

T
|
T

G

G

A

C

F
|
F

V

M

L

L

T

M

N

N

A

T

G

G

E

E

R

K

Q

A

P

V

A

K

S

S

K

E

H

N

R

G

L

L

S

T

T

T

V

I

L

A

W

C

Q

G

L

P

G

T

G

G

R

E

R

V

T

N

Y

Y

A

A

I

L

E

E

G

G

S

A

V

V

F

F

V

M

A

A

G
|
G

S

A

V

S

V
x
I

K
x
Q

W

W

L

L

R

R

D

D

S

E

L

M

G

K

L

L

I

I

E

N

S

D

A
|
A

G

Y

E

D

T

S

E

E

V

Y

L

F

A

A

R

T

S

K

V

V

P

Q

D

N

N

T

G

N

G

G

V

V

Y

Y

F

V

V

V

P

P

A

A

L

F

T

T

G

G

L

L

G

G

A

A

P

P

W

Y

W

W

E

D

P

P

E

Y

A

A

R

R

G

G

A

A

I

I

S

F

G

G

L

L

S

T

L

R

A

G

S

V

T

N

R

A

A

N

H

H

V

I

R

R

A

A

A

T

L

L

E

E

A

S

M

I

A

A

H

Y

Q

Q

S

T

H

R

D

D

L

V

K

L

A

E

A

A

F

M

A

Q

A

A

D

D

-

S

G

G

A

I

D

R

W

L

S

H

S

A

L

L

R

R

I

V

D

D

G

G

G
|
G

M

A

I

V

T

A

N
|
N

D

N

W

F

I

L

A

M

Q

Q

D

F

L

Q

A

S

D

D

V

I

L

L

D

G

L

T

T

R

V

V

E

E

R

R

P

P

D

E

F

V

T

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

M

Y

L

L

A

A

G

G

V

L

G

A

Y

V

G

G

L

F

F

W

A

Q

G

N

L

L

D

D

E

E

A

L

A

Q

A

E

M

-

R

K

G

A

K

V

V

I

E

E

R

R

-

E

F

F

A

R

P

P

G

G

M

I

K

E

V

T

E

T

A

E

R

R

D

N

A

Y

R

R

L

Y

A

A

G

G

W

W

K

K

A

K

A

A

V

V

E

K

G

R

V

A

M

M

A

A

binding phosphomethylphosphonic acid adenylate ester: T12 (= T12), T13 (= T13), G261 (= G263), T262 (= T264), G305 (= G307), I308 (≠ V310), Q309 (≠ K311), A321 (= A323), G406 (= G407), N410 (= N411)
binding glycerol: R82 (= R81), E83 (= E82), Y134 (= Y133), D240 (= D242), Q241 (= Q243), F265 (= F267)

1gljO Escherichia coli glycerol kinase mutant with bound atp analog showing substantial domain motion (see paper)
45% identity, 99% coverage: 5:491/491 of query aligns to 5:490/494 of 1gljO

query
sites
1gljO

L

I

L

V

V

A

I

L

D

D

E

Q

G

G

T
|
T

S

S

T

S

R

R

A

A

M

V

L

V

F

M

A

D

P

H

D

D

G

A

T

N

L

I

A

S

S

V

R

S

S

Q

A

R

E

E

L

F

A

E

Q

Q

H

I

Y

Y

P

P

A

K

P

P

G

G

L

W

V

V

E

E

H

H

D

D

A

P

A

M

E

E

I

I

W

W

-

A

E

T

K

Q

T

S

L

W

G

T

C

L

A

V

V

E

A

V

M

L

V

A

E

K

A

A

A

D

G

I

G

S

A

S

D

D

R

Q

I

I

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

I

T

V

I

F

V

W

W

D

E

K

K

A

E

T

T

G

G

E

K

P

P

L

I

A

Y

P

N

A

A

I

I

V

V

W
|
W

Q

Q

D

C

R

R

S

T

A

A

A

E

I

I

C

C

R

E

E

H

L

L

K

K

E

R

A

D

G

G

E

L

E

E

P

D

G

Y

V

I

Q

R

A

S

R

N

T

T

G

G

L

L

L

V

L

I

D

D

P

P

Y
|
Y

F

F

S

S

G

G

T

T

K

K

I

V

A

K

W

W

A

I

M

L

A

D

N

H

W

V

P

E

Q

G

L

S

R

R

E

E

A

R

G

A

D

R

R

R

-

G

-

E

L

L

A

L

I

F

G

G

T

T

V

V

E

D

S

T

W

W

L

L

V

I

W

W

K

K

L

M

T

T

A

-

N

-

E

-

K

-

R

Q

G

G

G

R

L

V

H

H

I

V

T

T

D

D

A

Y

T

T

N

N

A

A

S

S

R

R

T

T

L

M

L

L

M

F

G

N

L

I

G

H

S

T

G

L

G

D

W

W

S

D

D

D

G

K

L

M

I

L

D

E

L

V

F

L

G

D

A

I

P

P

R

R

A

E

A

M

L

L

A

P

E

E

I

V

V

R

D

R

C

S

A

S

G

E

R

V

F

Y

G

G

E

Q

T

T

T

N

L

I

F

-

G

G

G

T

P

R

I

I

P

P

I

I

C

S

G

G

M

I

A

A

G

G

D
|
D

Q

Q

A

A

T

L

I

F

G

G

Q

Q

A

L

C

C

L

V

S

K

P

E

G

G

D

M

T

A

K

K

A

N

T

T

F

Y

G
|
G

T
|
T

G

G

A

C

F
|
F

V

M

L

L

T

M

N

N

A

T

G

G

E

E

R

K

Q

A

P

V

A

K

S

S

K

E

H

N

R

G

L

L

S

T

T

T

V

I

L

A

W

C

Q

G

L

P

G

T

G

G

R

E

R

V

T

N

Y

Y

A

A

I

L

E

E

G

G

S

A

V

V

F

F

V

M

A

A

G
|
G

S

A

V

S

V

I

K
x
Q

W

W

L

L

R

R

D

D

S

E

L

M

G

K

L

L

I

I

E

N

S

D

A
|
A

G

Y

E

D

T

S

E

E

V

Y

L

F

A

A

R

T

S

K

V

V

P

Q

D

N

N

T

G

N

G

G

V

V

Y

Y

F

V

V

V

P

P

A

A

L

F

T

T

G

G

L

L

G

G

A

A

P

P

W

Y

W

W

E

D

P

P

E

Y

A

A

R

R

G

G

A

A

I

I

S

F

G

G

L

L

S

T

L

R

A

G

S

V

T

N

R

A

A

N

H

H

V

I

R

R

A

A

A

T

L

L

E

E

A

S

M

I

A

A

H

Y

Q

Q

S

T

H

R

D

D

L

V

K

L

A

E

A

A

F

M

A

Q

A

A

D

D

-

S

G

G

A

I

D

R

W

L

S

H

S

A

L

L

R

R

I

V

D

D

G

G

G
|
G

M
x
A

I

V

T

A

N

N

D

N

W

F

I

L

A

M

Q

Q

D

F

L

Q

A

S

D

D

V

I

L

L

D

G

L

T

T

R

V

V

E

E

R

R

P

P

D

E

F

V

T

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

M

Y

L

L

A

A

G

G

V

L

G

A

Y

V

G

G

L

F

F

W

A

Q

G

N

L

L

D

D

E

E

A

L

A

Q

A

E

M

-

R

K

G

A

K

V

V

I

E

E

R

R

-

E

F

F

A

R

P

P

G

G

M

I

K

E

V

T

E

T

A

E

R

R

D

N

A

Y

R

R

L

Y

A

A

G

G

W

W

K

K

A

K

A

A

V

V

E

K

G

R

V

A

M

M

A

A

binding gamma-arsono-beta, gamma-methyleneadenosine-5'-diphosphate: T12 (= T12), T13 (= T13), G261 (= G263), T262 (= T264), G305 (= G307), Q309 (≠ K311), A321 (= A323), G406 (= G407), A407 (≠ M408)
binding glycerol: R82 (= R81), E83 (= E82), W102 (= W101), Y134 (= Y133), D240 (= D242), F265 (= F267)

1bwfO Escherichia coli glycerol kinase mutant with bound atp analog showing substantial domain motion (see paper)
45% identity, 99% coverage: 5:491/491 of query aligns to 5:490/494 of 1bwfO

query
sites
1bwfO

L

I

L

V

V

A

I

L

D

D

E

Q

G

G

T
|
T

S

S

T

S

R

R

A

A

M

V

L

V

F

M

A

D

P

H

D

D

G

A

T

N

L

I

A

S

S

V

R

S

S

Q

A

R

E

E

L

F

A

E

Q

Q

H

I

Y

Y

P

P

A

K

P

P

G

G

L

W

V

V

E

E

H

H

D

D

A

P

A

M

E

E

I

I

W

W

-

A

E

T

K

Q

T

S

L

W

G

T

C

L

A

V

V

E

A

V

M

L

V

A

E

K

A

A

A

D

G

I

G

S

A

S

D

D

R

Q

I

I

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

I

T

V

I

F

V

W

W

D

E

K

K

A

E

T

T

G

G

E

K

P

P

L

I

A

Y

P

N

A

A

I

I

V

V

W
|
W

Q

Q

D

C

R

R

S

T

A

A

A

E

I

I

C

C

R

E

E

H

L

L

K

K

E

R

A

D

G

G

E

L

E

E

P

D

G

Y

V

I

Q

R

A

S

R

N

T

T

G

G

L

L

L

V

L

I

D

D

P

P

Y
|
Y

F

F

S

S

G

G

T

T

K

K

I

V

A

K

W

W

A

I

M

L

A

D

N

H

W

V

P

E

Q

G

L

S

R

R

E

E

A

R

G

A

D

R

R

R

-

G

-

E

L

L

A

L

I

F

G

G

T

T

V

V

E

D

S

T

W

W

L

L

V

I

W

W

K

K

L

M

T

T

A

-

N

-

E

-

K

-

R

Q

G

G

G

R

L

V

H

H

I

V

T

T

D

D

A

Y

T

T

N

N

A

A

S

S

R

R

T

T

L

M

L

L

M

F

G

N

L

I

G

H

S

T

G

L

G

D

W

W

S

D

D

D

G

K

L

M

I

L

D

E

L

V

F

L

G

D

A

I

P

P

R

R

A

E

A

M

L

L

A

P

E

E

I

V

V

R

D

R

C

S

A

S

G

E

R

V

F

Y

G

G

E

Q

T

T

T

N

L

I

F

-

G

G

G

T

P

R

I

I

P

P

I

I

C

S

G

G

M

I

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

T

L

I

F

G

G

Q

Q

A

L

C

C

L

V

S

K

P

E

G

G

D

M

T

A

K

K

A

N

T

T

F

Y

G

G

T
|
T

G

G

A

C

F
|
F

V

M

L

L

T

M

N

N

A

T

G

G

E

E

R

K

Q

A

P

V

A

K

S

S

K

E

H

N

R

G

L

L

S

T

T

T

V

I

L

A

W

C

Q

G

L

P

G

T

G

G

R

E

R

V

T

N

Y

Y

A

A

I

L

E

E

G

G

S

A

V

V

F

F

V

M

A

A

G
|
G

S

A

V

S

V
x
I

K
x
Q

W

W

L

L

R

R

D

D

S

E

L

M

G

K

L

L

I

I

E

N

S

D

A
|
A

G

Y

E

D

T

S

E

E

V

Y

L

F

A

A

R

T

S

K

V

V

P

Q

D

N

N

T

G

N

G

G

V

V

Y

Y

F

V

V

V

P

P

A

A

L

F

T

T

G

G

L

L

G

G

A

A

P

P

W

Y

W

W

E

D

P

P

E

Y

A

A

R

R

G

G

A

A

I

I

S

F

G

G

L

L

S

T

L

R

A

G

S

V

T

N

R

A

A

N

H

H

V

I

R

R

A

A

A

T

L

L

E

E

A

S

M

I

A

A

H

Y

Q

Q

S

T

H

R

D

D

L

V

K

L

A

E

A

A

F

M

A

Q

A

A

D

D

-

S

G

G

A

I

D

R

W

L

S

H

S

A

L

L

R

R

I

V

D

D

G

G

G
|
G

M

A

I

V

T

A

N
|
N

D

N

W

F

I

L

A

M

Q

Q

D

F

L

Q

A

S

D

D

V

I

L

L

D

G

L

T

T

R

V

V

E

E

R

R

P

P

D

E

F

V

T

R

E

E

T

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binding phosphodifluoromethylphosphonic acid-adenylate ester: T12 (= T12), T13 (= T13), T262 (= T264), G305 (= G307), I308 (≠ V310), Q309 (≠ K311), A321 (= A323), G406 (= G407), N410 (= N411)
binding glycerol: R82 (= R81), E83 (= E82), W102 (= W101), Y134 (= Y133), D240 (= D242), Q241 (= Q243), F265 (= F267)

P0A6F3 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Escherichia coli (strain K12) (see 10 papers)
45% identity, 100% coverage: 1:491/491 of query aligns to 1:496/502 of P0A6F3

query
sites
P0A6F3

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M1 (= M1) modified: Initiator methionine, Removed
T14 (= T12) binding ADP; binding sn-glycerol 3-phosphate
R18 (= R16) binding ADP
S59 (≠ G57) mutation to W: Abolishes inhibition of GK by FBP via disruption of the dimer-tetramer assembly reaction. Inhibition by EIIA-Glc is unchanged compared to wild type. The activity of this mutant is significantly higher than wild-type, and the Michaelis constants are increased slightly compared to wild-type.
A66 (≠ E64) mutation to T: Although it completely abolishes FBP regulation and disrupts dimer-tetramer equilibrium, the crystal structure is essentially identical to the symmetric tetramer found in the FBP-bound form of the enzyme.
R84 (= R81) binding glycerol; binding sn-glycerol 3-phosphate
E85 (= E82) binding glycerol; binding sn-glycerol 3-phosphate
Y136 (= Y133) binding glycerol; binding sn-glycerol 3-phosphate
G231 (≠ F230) mutation to D: Displays an increased enzymatic activity and a decreased allosteric regulation by FBP compared to wild-type. It displays a dimer form and is resistant to tetramer formation in the presence of FBP, whereas wild-type dimers are converted into inactive tetramers in the presence of FBP.
K233 (≠ G232) modified: N6-malonyllysine
G235 (vs. gap) binding beta-D-fructose 1,6-bisphosphate
R237 (≠ P233) binding beta-D-fructose 1,6-bisphosphate; mutation to A: Drastically reduces inhibition of GK by FBP and lowers, but did not eliminate, the ability of FBP to promote tetramer association.
D246 (= D242) binding glycerol; binding sn-glycerol 3-phosphate
Q247 (= Q243) binding glycerol
T268 (= T264) binding ADP
G305 (= G301) mutation to S: In glpK22; abolishes glucose control of glycerol utilization.
G311 (= G307) binding ADP
G412 (= G407) binding ADP
N416 (= N411) binding ADP
I475 (≠ M470) mutation to D: It decreases Vmax to about 10% of the wild-type value and the affinity for substrate is increased about two- to fourfold. This mutation decreases the catalytic activity in a manner that is analogous to that obtained upon EIIA-Glc binding. It increases the affinity for FBP about fivefold.
E479 (≠ A474) binding Zn(2+)
R480 (= R475) mutation to D: It decreases Vmax to about 10% of the wild-type value and the affinity for substrate is increased about two- to fourfold. This mutation decreases the catalytic activity in a manner that is analogous to that obtained upon EIIA-Glc binding. Regulation by FBP is not affected by this substitution. No inhibition by EIIA-Glc is observed, which is consistent with a decrease in affinity for EIIA-Glc of about 250-fold.

1bu6Y Crystal structures of escherichia coli glycerol kinase and the mutant a65t in an inactive tetramer: conformational changes and implications for allosteric regulation (see paper)
45% identity, 99% coverage: 5:491/491 of query aligns to 5:494/499 of 1bu6Y

query
sites
1bu6Y

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