SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ga0059261_0925 FitnessBrowser__Korea:Ga0059261_0925 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5mh6A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-ketohexanoic acid and NAD+ (1.35 a resolution)
38% identity, 78% coverage: 64:305/311 of query aligns to 59:305/306 of 5mh6A

query
sites
5mh6A

W

W

L

V

S

H

T

C

I

I

Y
x
R

A
|
A

G
|
G

I

Y

D

D

A

E

F

F

D

P

V

V

D

G

L

V

L

Y

K

E

T

E

R

A

G

G

T

T

I

Y

L

L

T

T

N

N

G

S

V
x
T

G

G

I

I

N
x
H

A

G

I

T

A

T

V

V

A

G

E

E

Y

T

A

V

V

A

M

G

G

Y

M

M

L

L

A

T

A

F

A

A

K

R

R

R

F

L

D

H

Q

A

V

Y

V

R

R

D

M

A

A

Q

D

H

R

D

R

H

E

A

W

W

P

-

A

-

D

D

A

L

P

P

G

R

K

Y

V

E

E

E

L

P

F

F

E
x
T

-

L

-
x
A

-

G

T

E

R

R

A

V

L

C

I

V

V

V

G

G

Y

L

G
|
G

T
|
T

I
x
L

G

G

R

R

M

G

I

V

G

V

D

D

R

R

L

A

A

A

F

L

G

G

V

M

D

E

V

V

T

V

G

G

V

V

T

R

R
|
R

S

S

G

G

R

D

-

P

-

V

-

D

-

N

-

V

D

S

G

T

T

V

L

Y

T

T

P

P

G

D

Q

R

W

L

R

H

E

E

R

A

L

I

G

A

D

D

Y

A

D

R

W

F

L

V

V

V

L

L

A

A

A

T

P
|
P

S

L

T

T

G

D

D

E

T
|
T

H

E

A

G

L

M

I

V

G

A

T

A

A

P

E

E

L
x
F

A
x
E

A

T

M
|
M

K

R

P

E

G

D

A

A

W

S

L

L

V

V

N

N

V
|
V

A
|
A

R
|
R

G
|
G

D
x
P

M

V

V
|
V

D

V

Q
x
E

P

S

A

D

L

L

I

V

E

A

A

A

L

L

E

D

K

S

R

G

R
x
D

I

I

A

A

G

G

A

A

F

A

L

L

D

D

V

V

V

F

D
x
S

P

E

E

E

P

P

L

L

P

P

D

E

D

D

H

S

P

P

L

L

W

W

T

D

T

F

P

E

N

D

V

V

I

L

H

I

S

T

M

P

H
|
H

L

V

S
|
S

G

A

R

-

S

A

Q

T

A

S

K

K

M
x
Y

F

H

M

E

R

D

A

V

A

A

L

L

F

I

L

R

E

E

N

N

A

I

R

E

A

K

F

I

A

A

E

T

G

G

R

D

P

E

M

L

K

T

N

N

V

R

V

V

binding 2-Ketohexanoic acid: R64 (≠ Y69), A65 (= A70), G66 (= G71), H89 (≠ N94), R224 (= R223), H272 (= H271), Y280 (≠ M280)
binding magnesium ion: T130 (≠ E137), A132 (vs. gap), F210 (≠ L209), E211 (≠ A210), M213 (= M212), G225 (= G224), P226 (≠ D225), V228 (= V227), E230 (≠ Q229), D241 (≠ R240), S251 (≠ D250)
binding nicotinamide-adenine-dinucleotide: A65 (= A70), G66 (= G71), T86 (≠ V91), H89 (≠ N94), G142 (= G146), T143 (= T147), L144 (≠ I148), R164 (= R168), P196 (= P195), T201 (= T200), V222 (= V221), A223 (= A222), R224 (= R223), H272 (= H271), S274 (= S273)

5mhaB D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
38% identity, 78% coverage: 64:305/311 of query aligns to 61:307/308 of 5mhaB

query
sites
5mhaB

W

W

L

V

S

H

T

C

I

I

Y
x
R

A
|
A

G
|
G

I

Y

D

D

A

E

F

F

D

P

V

V

D

G

L

V

L

Y

K

E

T

E

R

A

G

G

T

T

I

Y

L

L

T

T

N

N

G

S

V
x
T

G

G

I

I

N
x
H

A

G

I

T

A

T

V

V

A

G

E

E

Y

T

A

V

V

A

M

G

G

Y

M

M

L

L

A

T

A

F

A

A

K

R

R

R

F

L

D

H

Q

A

V

Y

V

R

R

D

M

A

A

Q

D

H

R

D

R

H

E

A

W

W

P

-

A

-

D

D

A

L

P

P

G

R

K

Y

V

E

E

E

L

P

F

F

E

T

-

L

-

A

-

G

T

E

R

R

A

V

L

C

I

V

V

V

G

G

Y
x
L

G
|
G

T
|
T

I
x
L

G

G

R

R

M

G

I

V

G

V

D

D

R

R

L

A

A

A

F

L

G

G

V

M

D

E

V

V

T

V

G

G

V

V

T
x
R

R
|
R

S
|
S

G

G

R

D

-

P

-

V

-

D

-

N

-

V

D

S

G

T

T

V

L

Y

T

T

P
|
P

G

D

Q

R

W

L

R

H

E

E

R

A

L

I

G

A

D

D

Y

A

D

R

W

F

L

V

V

V

L

L

A

A

A
x
T

P
|
P

S

L

T

T

G

D

D

E

T
|
T

H

E

A

G

L

M

I

V

G

A

T

A

A

P

E

E

L
x
F

A
x
E

A

T

M
|
M

K

R

P

E

G

D

A

A

W

S

L

L

V

V

N

N

V
|
V

A
|
A

R
|
R

G

G

D

P

M

V

V

V

D

V

Q

E

P

S

A

D

L

L

I

V

E

A

A

A

L

L

E

D

K

S

R

G

R
x
D

I

I

A

A

G

G

A

A

F

A

L

L

D

D

V

V

V

F

D

S

P

E

E

E

P

P

L

L

P

P

D

E

D

D

H

S

P

P

L

L

W

W

T

D

T

F

P

E

N

D

V

V

I

L

H

I

S

T

M

P

H
|
H

L

V

S
|
S

G

A

R

-

S

A

Q

T

A

S

K

K

M
x
Y

F

H

M

E

R

D

A

V

A

A

L

L

F

I

L

R

E

E

N

N

A

I

R

E

A

K

F

I

A

A

E

T

G

G

R

D

P

E

M

L

K

T

N

N

V

R

V

V

binding 2-Ketohexanoic acid: R66 (≠ Y69), R226 (= R223), H274 (= H271), Y282 (≠ M280)
binding (2R)-2-hydroxyhexanoic acid: R66 (≠ Y69), A67 (= A70), G68 (= G71), H91 (≠ N94), Y282 (≠ M280)
binding magnesium ion: F212 (≠ L209), E213 (≠ A210), M215 (= M212), D243 (≠ R240)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A67 (= A70), G68 (= G71), T88 (≠ V91), L143 (≠ Y145), G144 (= G146), T145 (= T147), L146 (≠ I148), R165 (≠ T167), R166 (= R168), S167 (= S169), P180 (= P177), T197 (≠ A194), P198 (= P195), T203 (= T200), V224 (= V221), A225 (= A222), R226 (= R223), H274 (= H271), S276 (= S273)

5mhaA D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
38% identity, 78% coverage: 64:305/311 of query aligns to 61:307/308 of 5mhaA

query
sites
5mhaA

W

W

L

V

S

H

T

C

I

I

Y
x
R

A
|
A

G
|
G

I

Y

D

D

A

E

F

F

D

P

V

V

D

G

L

V

L

Y

K

E

T

E

R

A

G

G

T

T

I

Y

L

L

T

T

N

N

G

S

V
x
T

G

G

I

I

N
x
H

A

G

I

T

A

T

V

V

A

G

E

E

Y

T

A

V

V

A

M

G

G

Y

M

M

L

L

A

T

A

F

A

A

K

R

R

R

F

L

D

H

Q

A

V

Y

V

R

R

D

M

A

A

Q

D

H

R

D

R

H

E

A

W

W

P

-

A

-

D

D

A

L

P

P

G

R

K

Y

V

E

E

E

L

P

F

F

E
x
T

-

L

-
x
A

-

G

T

E

R

R

A

V

L

C

I

V

V

V

G

G

Y
x
L

G
|
G

T
|
T

I
x
L

G

G

R

R

M

G

I

V

G

V

D

D

R

R

L

A

A

A

F

L

G

G

V

M

D

E

V

V

T

V

G

G

V

V

T
x
R

R
|
R

S
|
S

G

G

R

D

-

P

-

V

-

D

-

N

-

V

D

S

G

T

T

V

L

Y

T

T

P
|
P

G

D

Q

R

W

L

R

H

E

E

R

A

L

I

G

A

D

D

Y

A

D

R

W

F

L

V

V

V

L

L

A

A

A
x
T

P
|
P

S

L

T

T

G

D

D

E

T
|
T

H

E

A

G

L

M

I

V

G

A

T

A

A

P

E

E

L

F

A

E

A

T

M

M

K

R

P

E

G

D

A

A

W

S

L

L

V

V

N

N

V
|
V

A
|
A

R
|
R

G

G

D

P

M

V

V

V

D

V

Q

E

P

S

A

D

L

L

I

V

E

A

A

A

L

L

E

D

K

S

R

G

R

D

I

I

A

A

G

G

A

A

F

A

L

L

D

D

V

V

V

F

D

S

P

E

E

E

P

P

L

L

P

P

D

E

D

D

H

S

P

P

L

L

W

W

T

D

T

F

P

E

N

D

V

V

I

L

H

I

S

T

M

P

H
|
H

L

V

S
|
S

G

A

R

-

S

A

Q

T

A

S

K

K

M
x
Y

F

H

M

E

R

D

A

V

A

A

L

L

F

I

L

R

E

E

N

N

A

I

R

E

A

K

F

I

A

A

E

T

G

G

R

D

P

E

M

L

K

T

N

N

V

R

V

V

binding 2-Ketohexanoic acid: R66 (≠ Y69), A67 (= A70), G68 (= G71), H91 (≠ N94), R226 (= R223), H274 (= H271), Y282 (≠ M280)
binding magnesium ion: T132 (≠ E137), A134 (vs. gap)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A67 (= A70), G68 (= G71), T88 (≠ V91), L143 (≠ Y145), G144 (= G146), T145 (= T147), L146 (≠ I148), R165 (≠ T167), R166 (= R168), S167 (= S169), P180 (= P177), T197 (≠ A194), P198 (= P195), T203 (= T200), V224 (= V221), A225 (= A222), R226 (= R223), H274 (= H271), S276 (= S273)

5mh5A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-keto-hexanoic acid and NADP+ (1.4 a resolution)
38% identity, 78% coverage: 64:305/311 of query aligns to 61:307/308 of 5mh5A

query
sites
5mh5A

W

W

L

V

S

H

T

C

I

I

Y
x
R

A
|
A

G
|
G

I

Y

D

D

A

E

F

F

D

P

V

V

D

G

L

V

L

Y

K

E

T

E

R

A

G

G

T

T

I

Y

L

L

T

T

N

N

G

S

V
x
T

G

G

I

I

N
x
H

A

G

I

T

A

T

V

V

A

G

E

E

Y

T

A

V

V

A

M

G

G

Y

M

M

L

L

A

T

A

F

A

A

K

R

R

R

F

L

D

H

Q

A

V

Y

V

R

R

D

M

A

A

Q

D

H

R

D

R

H

E

A

W

W

P

-

A

-

D

D

A

L

P

P

G

R

K

Y

V

E

E

E

L

P

F

F

E
x
T

-

L

-
x
A

-

G

T

E

R

R

A

V

L

C

I

V

V

V

G
|
G

Y
x
L

G
|
G

T
|
T

I
x
L

G

G

R

R

M

G

I

V

G

V

D

D

R

R

L

A

A

A

F

L

G

G

V

M

D

E

V

V

T

V

G

G

V

V

T
x
R

R
|
R

S
|
S

G

G

R

D

-

P

-

V

-

D

-

N

-

V

D

S

G

T

T

V

L

Y

T

T

P

P

G

D

Q

R

W

L

R

H

E

E

R

A

L

I

G

A

D

D

Y

A

D

R

W

F

L

V

V

V

L

L

A

A

A
x
T

P
|
P

S

L

T

T

G

D

D

E

T
|
T

H

E

A

G

L

M

I

V

G

A

T

A

A

P

E

E

L
x
F

A
x
E

A

T

M
|
M

K

R

P

E

G

D

A

A

W

S

L

L

V

V

N

N

V
|
V

A
|
A

R
|
R

G

G

D

P

M

V

V

V

D

V

Q

E

P

S

A

D

L

L

I

V

E

A

A

A

L

L

E

D

K

S

R

G

R
x
D

I

I

A

A

G

G

A

A

F

A

L

L

D

D

V

V

V

F

D

S

P

E

E

E

P

P

L

L

P

P

D

E

D

D

H

S

P

P

L

L

W

W

T

D

T

F

P

E

N

D

V

V

I

L

H

I

S

T

M

P

H
|
H

L

V

S
|
S

G

A

R

-

S

A

Q

T

A

S

K

K

M
x
Y

F

H

M

E

R

D

A

V

A

A

L

L

F

I

L

R

E

E

N

N

A

I

R

E

A

K

F

I

A

A

E

T

G

G

R

D

P

E

M

L

K

T

N

N

V

R

V

V

binding 2-Ketohexanoic acid: R66 (≠ Y69), A67 (= A70), G68 (= G71), H91 (≠ N94), R226 (= R223), H274 (= H271), Y282 (≠ M280)
binding magnesium ion: T132 (≠ E137), A134 (vs. gap), F212 (≠ L209), E213 (≠ A210), M215 (= M212), D243 (≠ R240)
binding nadp nicotinamide-adenine-dinucleotide phosphate: A67 (= A70), G68 (= G71), T88 (≠ V91), G142 (= G144), L143 (≠ Y145), G144 (= G146), T145 (= T147), L146 (≠ I148), R165 (≠ T167), R166 (= R168), S167 (= S169), T197 (≠ A194), P198 (= P195), T203 (= T200), V224 (= V221), A225 (= A222), R226 (= R223), H274 (= H271), S276 (= S273)

5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
33% identity, 87% coverage: 29:298/311 of query aligns to 36:314/334 of 5aovA

query
sites
5aovA

R

R

E

E

E

K

A

L

I

L

A

E

M

K

V

V

A

K

D

D

A

V

D

D

I

-

A

A

W

L

V

V

D

T

M
|
M

M
x
L

R

S

P

E

D

R

W

I

T

D

G

Q

E

E

A

V

A

F

A

E

A

N

G

A

V

P

R

R

L

L

K

R

W

I

L

V

S

A

T

N

I
x
Y

Y
x
A

A
x
V

G
|
G

I

Y

D

D

A

N

F

I

D

D

V

V

D

E

L

E

L

A

K

T

T

R

R

R

G

G

T

I

I

Y

L

V

T

T

N

N

G

T

V

P

G

D

I

V

N
x
L

A

T

I

N

A

A

V
x
T

A

A

E

D

Y

H

A

A

V

F

M

A

G

L

M

L

L

L

A

A

A

T

A

A

K

R

R

H

F

V

-

V

-

K

-

G

D

D

Q

K

V

F

V

V

R

R

M

S

A

G

D

E

-

W

R

K

R

R

E

K

W

G

P

I

A

A

D

W

A

H

P

P

G

K

-

W

-

F

-

L

K

G

V

Y

E

E

L

L

F

Y

E

G

T

K

R

T

A

I

L

G

I

I

V

V

G

G

Y
x
F

G
|
G

T
x
R

I
|
I

G

G

R

Q

M

A

I

I

G

A

D

R

R

R

L

A

A

K

A

G

F

F

G

N

V

M

D

R

V

I

T

L

G

Y

V

Y

T
x
S

R
|
R

S

T

G

R

R

K

D

S

G

Q

T

A

L

E

T

K

P

E

-

L

-

G

G

A

Q

E

W

Y

R

R

-

P

-

L

-

E

E

E

R

V

L

L

G

K

D

E

Y

S

D

D

W

F

L

V

V

I

L

L

A
|
A

A
x
V

P
|
P

S

L

T

T

G

K

D

E

T
|
T

H

M

A

Y

L

M

I

I

G

N

T

E

A

E

E

R

L

L

A

K

A

L

M

M

K

K

P

P

G

T

A

A

W

I

L

L

V

V

N

N

V
x
I

A
|
A

R
|
R

G

G

D

K

M

V

V

V

D

D

Q

T

P

K

A

A

L

L

I

I

E

K

A

A

L

L

E

K

K

E

R

G

R

W

I

I

A

A

G

G

A

A

F

G

L

L

D
|
D

V

V

V

F

D

E

P

E

E
|
E

P

P

L

Y

P

Y

D

N

D

E

H

E

P

-

L

L

W

F

T

S

T

L

P

D

N

N

V

V

I

V

H

L

S

T

M

P

H
|
H

L

I

-
x
G

S

S

G

A

R

T

S

F

Q

E

A

A

K

R

M

E

F

A

M

M

R

-

A

-

A

A

A

E

L

L

F

V

L

A

E

R

N

N

A

L

R

I

A

A

F

F

A

K

E

R

G

G

active site: L100 (≠ N94), R241 (= R223), D265 (= D247), E270 (= E252), H288 (= H271)
binding glyoxylic acid: M52 (= M46), L53 (≠ M47), L53 (≠ M47), Y74 (≠ I68), A75 (≠ Y69), V76 (≠ A70), G77 (= G71), R241 (= R223), H288 (= H271)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ A70), T104 (≠ V98), F158 (≠ Y145), G159 (= G146), R160 (≠ T147), I161 (= I148), S180 (≠ T167), R181 (= R168), A211 (= A193), V212 (≠ A194), P213 (= P195), T218 (= T200), I239 (≠ V221), A240 (= A222), R241 (= R223), H288 (= H271), G290 (vs. gap)

Q9UBQ7 Glyoxylate reductase/hydroxypyruvate reductase; EC 1.1.1.79; EC 1.1.1.81 from Homo sapiens (Human) (see paper)
32% identity, 81% coverage: 57:307/311 of query aligns to 70:328/328 of Q9UBQ7

query
sites
Q9UBQ7

A

A

G

G

V

A

R

N

L

L

K

K

W

V

L

I

S

S

T

T

I

M

Y

S

A
x
V

G
|
G

I

I

D

D

A

H

F

L

D

A

V

L

D

D

L

E

L

I

K

K

T

K

R

R

G

G

T

I

I

R

L

V

T

G

N

Y

G

T

V

P

G

D

I

V

N

L

A

T

I

D

A

T

V

T

A

A

E

E

Y

L

A

A

V

V

M

S

G

L

M

L

L

L

A

T

A

C

K

R

R

R

F

L

D

P

Q

E

V

A

V

I

R

E

M

E

A

V

D

K

R

N

R

G

E

G

W

W

P

T

A

S

D

W

A

K

P

P

-

L

-

W

-

L

G

C

K

G

V

Y

E

G

L

L

F

T

E

Q

T

S

R

T

A

V

L

G

I

I

V

I

G

G

Y

L

G
|
G

T
x
R

I
|
I

G

G

R

Q

M

A

I

I

G

A

D

R

R

R

L

L

A

K

A

P

F

F

G

G

V

V

D

Q

V

R

T

F

G

L

V

Y

T

T

-

G

R
|
R

S
x
Q

G
x
P

R
|
R

D

P

G

E

T

E

L

A

T

A

P

E

G

F

Q

Q

W

A

R

E

-

F

-

V

-

S

-

T

-

P

E

E

R

L

L

A

G

A

D

Q

Y

S

D

D

W

F

L

I

V

V

L

V

A

A

A

C

P
x
S

S

L

T

T

G

P

D

A

T

T

H

E

A

G

L

L

I

C

G

N

T

K

A

D

E

F

L

F

A

Q

A

K

M

M

K

K

P

E

G

T

A

A

W

V

L

F

V

I

N

N

V
x
I

A

S

R
|
R

G

G

D

D

M

V

V

V

D

N

Q

Q

P

D

A

D

L

L

I

Y

E

Q

A

A

L

L

E

A

K

S

R

G

R

K

I

I

A

A

G

A

A

A

F

G

L

L

D
|
D

V

V

V

T

D

S

P

P

E

E

P

P

L

L

P

P

D

T

D

N

H

H

P

P

L

L

W

L

T

T

T

L

P

K

N

N

V

C

I

V

H

I

S

L

M

P

H
|
H

L
x
I

-
x
G

-
x
S

-

A

S

T

G

H

R

R

S

T

Q

R

A

N

K

T

M

M

F

S

M

L

R

L

A

A

A

N

L

N

F

L

L

L

E

A

N

G

A

L

R

R

A

-

F

-

A

-

E

-

G

G

R

E

P

P

M

M

K

P

N

S

V

E

V

L

D

K

L

L

VG 83:84 (≠ AG 70:71) binding substrate
GRI 162:164 (≠ GTI 146:148) binding NADP(+)
RQPR 185:188 (≠ RSGR 168:171) binding NADP(+)
S217 (≠ P195) binding NADP(+)
I243 (≠ V221) binding NADP(+)
R245 (= R223) binding substrate
D269 (= D247) binding substrate
HIGS 293:296 (≠ HL-- 271:272) binding substrate
G295 (vs. gap) binding NADP(+)

2gcgA Ternary crystal structure of human glyoxylate reductase/hydroxypyruvate reductase (see paper)
32% identity, 81% coverage: 57:307/311 of query aligns to 66:324/324 of 2gcgA

query
sites
2gcgA

A

A

G

G

V

A

R

N

L

L

K

K

W

V

L

I

S

S

T

T

I

M

Y
x
S

A
x
V

G
|
G

I

I

D

D

A

H

F

L

D

A

V

L

D

D

L

E

L

I

K

K

T

K

R

R

G

G

T

I

I

R

L

V

T

G

N

Y

G

T

V

P

G

D

I

V

N
x
L

A

T

I

D

A

T

V
x
T

A

A

E

E

Y

L

A

A

V

V

M

S

G

L

M

L

L

L

A

T

A

C

K

R

R

R

F

L

D

P

Q

E

V

A

V

I

R

E

M

E

A

V

D

K

R

N

R

G

E

G

W

W

P

T

A

S

D

W

A

K

P

P

-

L

-

W

-

L

G

C

K

G

V

Y

E

G

L

L

F

T

E

Q

T

S

R

T

A

V

L

G

I

I

V

I

G
|
G

Y

L

G
|
G

T

R

I
|
I

G

G

R

Q

M

A

I

I

G

A

D

R

R

R

L

L

A

K

A

P

F

F

G

G

V

V

D

Q

V

R

T

F

G

L

V

Y

T

T

-
x
G

R
|
R

S

Q

G

P

R
|
R

D

P

G

E

T

E

L

A

T

A

P

E

G

F

Q

Q

W

A

R

E

-

F

-

V

-

S

-

T

-

P

E

E

R

L

L

A

G

A

D

Q

Y

S

D

D

W

F

L

I

V

V

L

V

A

A

A
x
C

P
x
S

S

L

T

T

G

P

D

A

T
|
T

H

E

A

G

L

L

I

C

G

N

T

K

A

D

E

F

L

F

A

Q

A

K

M

M

K

K

P

E

G

T

A

A

W

V

L

F

V

I

N

N

V
x
I

A

S

R
|
R

G

G

D

D

M

V

V

V

D

N

Q

Q

P

D

A

D

L

L

I

Y

E

Q

A

A

L

L

E

A

K

S

R

G

R

K

I

I

A

A

G

A

A

A

F

G

L

L

D
|
D

V

V

V

T

D

S

P

P

E
|
E

P

P

L

L

P

P

D

T

D

N

H

H

P

P

L

L

W

L

T

T

T

L

P

K

N

N

V

C

I

V

H

I

S

L

M

P

H
|
H

L

I

-
x
G

-

S

-

A

S

T

G

H

R

R

S

T

Q

R

A

N

K

T

M

M

F

S

M

L

R

L

A

A

A

N

L

N

F

L

L

L

E

A

N

G

A

L

R

R

A

-

F

-

A

-

E

-

G

G

R

E

P

P

M

M

K

P

N

S

V

E

V

L

D

K

L

L

active site: L103 (≠ N94), R241 (= R223), D265 (= D247), E270 (= E252), H289 (= H271)
binding (2r)-2,3-dihydroxypropanoic acid: S78 (≠ Y69), V79 (≠ A70), G80 (= G71), R241 (= R223), H289 (= H271)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V79 (≠ A70), T107 (≠ V98), G156 (= G144), G158 (= G146), I160 (= I148), G180 (vs. gap), R181 (= R168), R184 (= R171), C212 (≠ A194), S213 (≠ P195), T218 (= T200), I239 (≠ V221), R241 (= R223), D265 (= D247), H289 (= H271), G291 (vs. gap)

Sites not aligning to the query:

binding (2r)-2,3-dihydroxypropanoic acid: 55

6ttbA Crystal structure of NAD-dependent formate dehydrogenase from staphylococcus aureus in complex with NAD
31% identity, 72% coverage: 52:274/311 of query aligns to 69:295/331 of 6ttbA

query
sites
6ttbA

T

T

G

R

E

E

A

R

A

I

A

E

A

K

G

A

V

P

R

N

L

L

K

K

W

L

L

A

S

I

T

T

I

A

Y

G

A
x
V

G

G

I

S

D

D

A

H

F

V

D

D

V

L

D

A

L

A

K

S

T

E

R

H

G

N

T

I

I

G

L

V

T

V

N

E

G

V

V

T

G

G

I

S

N
|
N

A

T

I

V

A

S

V
|
V

A

A

E

E

Y

H

A

A

V

V

M

M

G

D

M

L

L

L

A

I

A

L

K

R

R

N

F

Y

D

E

Q

E

V

G

V

H

R

R

M

Q

A

S

D

V

R

E

R

G

E

E

W

W

P

N

A

L

D

S

A

Q

P

V

G

G

K

N

V

H

-

A

-

H

E

E

L

L

F

Q

E

H

T

K

R

T

A

I

L

G

I

I

V
x
F

G

G

Y

F

G
|
G

T
x
R

I
|
I

G

G

R

Q

M

L

I

V

G

A

D

E

R

R

L

L

A

A

A

P

F

F

G

N

V

V

D

T

V

L

T

Q

G

H

V
x
Y

T
x
D

R
x
P

-

I

S

N

G

Q

R

Q

D

D

G

H

T

K

L

L

T

S

P

K

G

F

-

V

Q

S

W

F

R

D

E

E

R

L

L

V

G

S

D

S

Y

S

D

D

W

A

L

I

V

T

L

I

A
x
H

A
|
A

P
|
P

S

L

T

T

G

P

D

E

T
|
T

H

D

A

N

L

L

I

F

G

D

T

K

A

D

E

V

L

L

A

S

A

R

M

M

K

K

P

K

G

H

A

S

W

Y

L

L

V

V

N

N

V
x
T

A
|
A

R
|
R

G

G

D

K

M

I

V

V

D

N

Q

R

P

D

A

A

L

L

I

V

E

E

A

A

L

L

E

A

K

S

R

E

R

H

I

L

A

Q

G

G

A

Y

F

A

L

G

D

D

V

V

V

W

D

Y

P

P

E

Q

P

P

L

A

P

P

D

A

D

D

H

H

P

P

L

W

W

R

T

T

T

M

P

P

N

R

V

N

I

A

H

M

S

T

M

V

H
|
H

L

Y

S
|
S

G
|
G

binding nicotinamide-adenine-dinucleotide: V87 (≠ A70), N111 (= N94), V115 (= V98), F162 (≠ V143), G165 (= G146), R166 (≠ T147), I167 (= I148), Y185 (≠ V166), D186 (≠ T167), P187 (≠ R168), H214 (≠ A193), A215 (= A194), P216 (= P195), T221 (= T200), T242 (≠ V221), A243 (= A222), R244 (= R223), H292 (= H271), S294 (= S273), G295 (= G274)

3bazA Structure of hydroxyphenylpyruvate reductase from coleus blumei in complex with NADP+ (see paper)
30% identity, 77% coverage: 61:301/311 of query aligns to 65:306/311 of 3bazA

query
sites
3bazA

R

K

L

L

K

E

W

I

L

V

S

S

T

S

I

F

Y

S

A
x
V

G

G

I

L

D

D

A

K

F

V

D

D

V

L

D

I

L

K

L

C

K

E

T

E

R

K

G

G

T

V

I

R

L

V

T

T

N

N

G

T

V

P

G

D

I

V

N
x
L

A

T

I

D

A

D

V

V

A

A

E

D

Y

L

A

A

V

I

M

G

G

L

M

I

L

L

A

A

A

V

A

L

K

R

R

R

F

I

D

C

Q

E

V

C

V

D

R

K

M

Y

A

V

D

R

R

R

R

G

E

A

W

W

P

K

-

F

A

G

D

D

A

F

P

K

G

L

K

T

V

T

E

K

L

F

F

S

E

G

T

K

R

R

A

V

L

G

I

I

V

I

G
|
G

Y
x
L

G
|
G

T
x
R

I
|
I

G

G

R

L

M

A

I

V

G

A

D

E

R

R

L

A

A

E

A

A

F

F

G

D

V

C

D

P

V

I

T

S

G

Y

V

F

T
x
S

R
|
R

S
|
S

G

K

R

K

D

P

G

N

T

T

-

N

-

Y

L

T

T

Y

P

Y

G

G

Q

S

W

V

R

V

E

E

R

L

L

A

G

S

D

N

Y

S

D

D

W

I

L

L

V

V

L

V

A

A

A
x
C

P
|
P

S

L

T

T

G

P

D

E

T
|
T

H

T

A

H

L

I

I

I

G

N

T

R

A

E

E

V

L

I

A

D

A

A

M

L

K

G

P

P

G

K

A

G

W

V

L

L

V

I

N

N

V
x
I

A
x
G

R
|
R

G

G

D

P

M

H

V

V

D

D

Q

E

P

P

A

E

L

L

I

V

E

S

A

A

L

L

E

V

K

E

R

G

R

R

I

L

A

G

G

G

A
|
A

F

G

L

L

D
|
D

V

V

V

F

D

E

P

R

E
|
E

P

P

-

E

L

V

P

P

D

E

D

K

H

-

P

-

L

L

W

F

T

G

T

L

P

E

N

N

V

V

I

V

H

L

S

L

M

P

H
|
H

L

V

-
x
G

S

S

G

G

R

T

S

V

Q

E

A

T

K

R

M

K

F

V

M

M

R

-

A

-

A

A

A

D

L

L

F

V

L

V

E

G

N

N

A

L

R

E

A

A

F

H

A

F

E

S

G

G

R

K

P

P

M

L

active site: L98 (≠ N94), R230 (= R223), A251 (= A244), D254 (= D247), E259 (= E252), H277 (= H271)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V74 (≠ A70), G149 (= G144), L150 (≠ Y145), G151 (= G146), R152 (≠ T147), I153 (= I148), S172 (≠ T167), R173 (= R168), S174 (= S169), C201 (≠ A194), P202 (= P195), T207 (= T200), I228 (≠ V221), G229 (≠ A222), R230 (= R223), D254 (= D247), H277 (= H271), G279 (vs. gap)

Q65CJ7 Hydroxyphenylpyruvate reductase; HPPR; EC 1.1.1.237 from Plectranthus scutellarioides (Coleus) (Solenostemon scutellarioides) (see paper)
30% identity, 77% coverage: 61:301/311 of query aligns to 67:308/313 of Q65CJ7

query
sites
Q65CJ7

R

K

L

L

K

E

W

I

L

V

S

S

T

S

I

F

Y

S

A

V

G

G

I

L

D

D

A

K

F

V

D

D

V

L

D

I

L

K

L

C

K

E

T

E

R

K

G

G

T

V

I

R

L

V

T

T

N

N

G

T

V

P

G

D

I

V

N

L

A

T

I

D

A

D

V

V

A

A

E

D

Y

L

A

A

V

I

M

G

G

L

M

I

L

L

A

A

A

V

A

L

K

R

R

R

F

I

D

C

Q

E

V

C

V

D

R

K

M

Y

A

V

D

R

R

R

R

G

E

A

W

W

P

K

-

F

A

G

D

D

A

F

P

K

G

L

K

T

V

T

E

K

L

F

F

S

E

G

T

K

R

R

A

V

L

G

I

I

V

I

G

G

Y
x
L

G
|
G

T
x
R

I
|
I

G

G

R

L

M

A

I

V

G

A

D

E

R

R

L

A

A

E

A

A

F

F

G

D

V

C

D

P

V

I

T

S

G

Y

V

F

T
x
S

R
|
R

S
|
S

G

K

R

K

D

P

G

N

T

T

-

N

-

Y

L

T

T

Y

P

Y

G

G

Q

S

W

V

R

V

E

E

R

L

L

A

G

S

D

N

Y

S

D

D

W

I

L

L

V

V

L

V

A

A

A

C

P

P

S

L

T

T

G

P

D

E

T

T

H

T

A

H

L

I

I

I

G

N

T

R

A

E

E

V

L

I

A

D

A

A

M

L

K

G

P

P

G

K

A

G

W

V

L

L

V

I

N

N

V
x
I

A

G

R

R

G

G

D

P

M

H

V

V

D

D

Q

E

P

P

A

E

L

L

I

V

E

S

A

A

L

L

E

V

K

E

R

G

R

R

I

L

A

G

G

G

A

A

F

G

L

L

D
|
D

V

V

V

F

D

E

P

R

E

E

P

P

-

E

L

V

P

P

D

E

D

K

H

-

P

-

L

L

W

F

T

G

T

L

P

E

N

N

V

V

I

V

H

L

S

L

M

P

H

H

L

V

-

G

S

S

G

G

R

T

S

V

Q

E

A

T

K

R

M

K

F

V

M

M

R

-

A

-

A

A

A

D

L

L

F

V

L

V

E

G

N

N

A

L

R

E

A

A

F

H

A

F

E

S

G

G

R

K

P

P

M

L

LGRI 152:155 (≠ YGTI 145:148) binding NADP(+)
SRS 174:176 (≠ TRS 167:169) binding NADP(+)
I230 (≠ V221) binding NADP(+)
D256 (= D247) binding NADP(+)

6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
34% identity, 87% coverage: 29:298/311 of query aligns to 35:313/332 of 6biiA

query
sites
6biiA

R

R

E

E

E

V

A

L

I

L

A

E

M

K

V

V

A

K

D

D

A

V

D

D

I

-

A

A

W

L

V

V

D

T

M

M

M

L

R

S

P

E

D

K

W

I

T

D

G

R

E

E

A

V

A

F

A

D

A

A

G

A

V

P

R

R

L

L

K

R

W

I

L

V

S

A

T

N

I

Y

Y

A

A
x
V

G

G

I

Y

D

D

A

N

F

I

D

D

V

I

D

E

L

E

L

A

K

T

T

K

R

R

G

G

T

I

I

Y

L

V

T

T

N

N

G

T

V

P

G

D

I

V

N
x
L

A

T

I

D

A

A

V
x
T

A

A

E

D

Y

L

A

A

V

W

M

A

G

L

M

L

L

L

A

A

K

R

R

H

F

V

-

V

-

K

-

G

D

D

Q

K

V

F

V

V

R

R

M

S

A

G

D

E

-

W

-

K

R

R

-

G

-

I

E

A

W

W

P

H

A

P

D

K

A

M

P

F

G

L

K

G

V

Y

E

D

L

V

F

Y

E

G

T

K

R

T

A

I

L

G

I

I

V

V

G
|
G

Y
x
F

G
|
G

T
x
R

I
|
I

G

G

R

Q

M

A

I

I

G

A

D

K

R

R

L

A

A

K

A

G

F

F

G

G

V

M

D

R

V

I

T

L

G

Y

V

T

T
x
A

R
|
R

S
|
S

G

R

R
x
K

-

P

-

E

-

A

-

E

-

K

-

E

-

L

D

G

G

A

T

E

L

F

T

K

P

P

G

L

Q

E

W

-

R

-

E

E

R

L

L

L

G

R

D

E

Y

S

D

D

W

F

L

V

V

V

L

L

A

A

A
x
V

P
|
P

S

L

T

T

G

K

D
x
E

T
|
T

H

Y

A

H

L

M

I

I

G

N

T

E

A

E

E

R

L

L

A

R

A

L

M

M

K

K

P

P

G

T

A

A

W

V

L

L

V

V

N

N

V
|
V

A
|
A

R
|
R

G

G

D

K

M

V

V

V

D

D

Q

T

P

K

A

A

L

L

I

I

E

R

A

A

L

L

E

K

K

E

R

G

R

W

I

I

A

A

G

A

A

A

F

G

L

L

D
|
D

V

V

V

F

D

E

P

E

E
|
E

P

P

L

Y

P

Y

D

D

D

E

H

E

P

-

L

L

W

F

T

A

T

L

P

D

N

N

V

V

I

V

H

L

S

T

M

P

H
|
H

L

I

-
x
G

S

S

G

A

R

T

S

F

Q

G

A

A

K

R

M

E

F

G

M

M

R

-

A

-

A

A

A

E

L

L

F

V

L

A

E

K

N

N

A

L

R

I

A

A

F

F

A

K

E

N

G

G

active site: L99 (≠ N94), R240 (= R223), D264 (= D247), E269 (= E252), H287 (= H271)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (≠ A70), T103 (≠ V98), G156 (= G144), F157 (≠ Y145), G158 (= G146), R159 (≠ T147), I160 (= I148), A179 (≠ T167), R180 (= R168), S181 (= S169), K183 (≠ R171), V211 (≠ A194), P212 (= P195), E216 (≠ D199), T217 (= T200), V238 (= V221), A239 (= A222), R240 (= R223), D264 (= D247), H287 (= H271), G289 (vs. gap)

2nadA High resolution structures of holo and apo formate dehydrogenase (see paper)
30% identity, 86% coverage: 36:303/311 of query aligns to 87:363/391 of 2nadA

query
sites
2nadA

V

L

A

V

D

D

A

A

D

D

I

V

A

V

W

-

V

-

D

-

M

I

M

S

R

Q

P
|
P

D
x
F

W

W

-

P

-

A

-

Y

-

L

T

T

G

P

E

E

A

R

A

I

A

A

A

K

G

A

V

K

R

N

L

L

K

K

W

L

L

A

S

L

T

T

I

A

Y

G

A
x
I

G

G

I

S

D

D

A

H

F

V

D

D

V

L

D

Q

L

S

L

A

K

I

T

D

R

R

G

N

T

V

I

T

L

V

T

A

N

E

G

V

V

T

G

Y

I

C

N
|
N

A

S

I

I

A

S

V
|
V

A

A

E

E

Y

H

A

V

V

V

M

M

G

M

M

I

L

L

A

S

A

L

A

V

K

R

R

N

F

Y

D

L

Q

P

V

S

V

H

R

E

M

W

A

A

D

R

R

K

R

G

E

G

W

W

P

N

A

I

D

-

A

A

P

D

G

C

K

V

V

S

E

H

L

A

F

Y

E

D

T

L

R

E

A

A

L

M

I

H

V

V

G

G

Y

T

-

V

-
x
A

-

A

G
|
G

T
x
R

I
|
I

G

G

R

L

M

A

I

V

G

L

D

R

R

R

L

L

A

A

A

P

F

F

G

D

V

V

D

H

V

L

T

H

G

Y

V

T

T
x
D

R

R

S

H

-

R

-

L

-

P

-

E

-

S

-

V

G

E

R

K

D

E

G

L

T

N

L

L

T

T

P

W

G

H

Q

A

W

T

R

R

E

E

R

D

L

M

G

Y

D

P

-

V

Y

C

D

D

W

V

L

V

V

T

L

L

A

N

A

C

P
|
P

S

L

T

H

G

P

D
x
E

T
|
T

H

E

A

H

L

M

I

I

G

N

T

D

A

E

E

T

L

L

A

K

A

L

M

F

K

K

P

R

G

G

A

A

W

Y

L

I

V

V

N

N

V
x
T

A
|
A

R
|
R

G

G

D

K

M

L

V

C

D

D

Q

R

P

D

A

A

L

V

I

A

E

R

A

A

L

L

E

E

K

S

R

G

R

R

I

L

A

A

G

G

A

Y

F

A

L

G

D
|
D

V

V

V

W

D

F

P

P

E
x
Q

P

P

L

A

P

P

D

K

D

D

H

H

P

P

L

W

W

R

T

T

T

M

P

P

N

Y

V

N

I

G

H

M

S

T

M

P

H
|
H

L

I

S
|
S

G
|
G

R

T

S

T

Q

L

A

T

K

A

M

Q

F

-

M

A

R

R

A

Y

A

A

L

G

F

T

L

R

E

E

N

I

A

L

R

E

A

C

F

F

A

F

E

E

G

G

R

R

P

P

M

I

K

R

N

D

active site: N146 (= N94), R284 (= R223), D308 (= D247), Q313 (≠ E252), H332 (= H271)
binding azide ion: P97 (= P49), F98 (≠ D50), I122 (≠ A70), N146 (= N94), R284 (= R223)
binding nicotinamide-adenine-dinucleotide: I122 (≠ A70), N146 (= N94), V150 (= V98), A198 (vs. gap), G200 (= G146), R201 (≠ T147), I202 (= I148), D221 (≠ T167), P256 (= P195), E260 (≠ D199), T261 (= T200), T282 (≠ V221), A283 (= A222), R284 (= R223), D308 (= D247), H332 (= H271), S334 (= S273), G335 (= G274)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 380

P33160 Formate dehydrogenase; FDH; NAD-dependent formate dehydrogenase; EC 1.17.1.9 from Pseudomonas sp. (strain 101) (Achromobacter parvulus T1) (see 3 papers)
30% identity, 86% coverage: 36:303/311 of query aligns to 88:364/401 of P33160

query
sites
P33160

V

L

A

V

D

D

A

A

D

D

I

V

A

V

W

-

V

-

D

-

M

I

M

S

R

Q

P

P

D

F

W

W

-

P

-

A

-

Y

-

L

T

T

G

P

E

E

A

R

A

I

A

A

A

K

G

A

V

K

R

N

L

L

K

K

W

L

L

A

S

L

T

T

I

A

Y

G

A
x
I

G

G

I

S

D

D

A

H

F

V

D

D

V

L

D

Q

L

S

L

A

K

I

T

D

R

R

G

N

T

V

I

T

L

V

T

A

N

E

G

V

V

T

G

Y

I

C

N
|
N

A
x
S

I

I

A

S

V

V

A

A

E

E

Y

H

A

V

V

V

M

M

G

M

M

I

L

L

A

S

A

L

A

V

K

R

R

N

F

Y

D

L

Q

P

V

S

V

H

R

E

M

W

A

A

D

R

R

K

R

G

E

G

W

W

P

N

A

I

D

-

A

A

P

D

G

C

K

V

V

S

E

H

L

A

F

Y

E

D

T

L

R

E

A

A

L

M

I

H

V

V

G

G

Y

T

-

V

-

A

G

G

T
x
R

I
|
I

G

G

R

L

M

A

I

V

G

L

D

R

R

R

L

L

A

A

A

P

F

F

G

D

V

V

D

H

V

L

T

H

G

Y

V

T

T
x
D

R

R

S

H

-

R

-

L

-

P

-

E

-

S

-

V

G

E

R

K

D

E

G

L

T

N

L

L

T

T

P

W

G

H

Q

A

W

T

R

R

E

E

R

D

L

M

G

Y

D

P

-

V

Y

C

D

D

W

V

L

V

V

T

L

L

A

N

A
x
C

P
|
P

S
x
L

T
x
H

G
x
P

D
x
E

T

T

H

E

A

H

L

M

I

I

G

N

T

D

A

E

E

T

L

L

A

K

A

L

M

F

K

K

P

R

G

G

A

A

W

Y

L

I

V

V

N

N

V
x
T

A

A

R

R

G

G

D

K

M

L

V

C

D

D

Q

R

P

D

A

A

L

V

I

A

E

R

A

A

L

L

E

E

K

S

R

G

R

R

I

L

A

A

G

G

A

Y

F

A

L

G

D
|
D

V

V

V

W

D

F

P

P

E

Q

P

P

L

A

P

P

D

K

D

D

H

H

P

P

L

W

W

R

T

T

T

M

P

P

N

Y

V

N

I

G

H

M

S

T

M

P

H
|
H

L
x
I

S
|
S

G
|
G

R

T

S

T

Q

L

A

T

K

A

M

Q

F

-

M

A

R

R

A

Y

A

A

L

G

F

T

L

R

E

E

N

I

A

L

R

E

A

C

F

F

A

F

E

E

G

G

R

R

P

P

M

I

K

R

N

D

I123 (≠ A70) binding substrate
N147 (= N94) binding substrate
S148 (≠ A95) binding NAD(+)
RI 202:203 (≠ TI 147:148) binding NAD(+)
D222 (≠ T167) binding NAD(+)
C256 (≠ A194) mutation C->S,M: High resistance to inactivation by Hg(2+), Increased stability at 25 degree Celsius and decreased thermostability at 45 degree Celsius.
PLHPE 257:261 (≠ PSTGD 195:199) binding NAD(+)
T283 (≠ V221) binding NAD(+)
D309 (= D247) binding NAD(+)
HISG 333:336 (≠ HLSG 271:274) binding NAD(+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
381 binding NAD(+)

P0A9T0 D-3-phosphoglycerate dehydrogenase; PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Escherichia coli (strain K12) (see 2 papers)
29% identity, 74% coverage: 48:277/311 of query aligns to 62:298/410 of P0A9T0

query
sites
P0A9T0

R

R

P

T

D

H

W

L

T

T

G

E

E

D

A

V

A

I

A

N

A

A

G

A

V

E

R

K

L

L

K

V

W

A

L

I

S

G

T

C

I

F

Y

C

A

I

G

G

I

T

D

N

A

Q

F

V

D

D

V

L

D

D

L

A

K

A

T

K

R

R

G

G

T

I

I

P

L

V

T

F

N

N

G

A

V

P

G

F

I

S

N

N

A

T

I

R

A

S

V

V

A

A

E

E

Y

L

A

V

V

I

M

G

G

E

M

L

L

L

A

L

K

R

R

G

F

V

D

P

Q

E

V

A

V

N

R

A

M

K

A

A

D

H

R

R

R

G

E

V

W

W

P

N

A

K

D

L

A

A

P

A

G

G

K

S

V

F

E

E

L

A

F

R

E

G

T

K

R

K

A

L

L

G

I

I

V

I

G

G

Y

Y

G

G

T
x
H

I
|
I

G

G

R

T

M

Q

I

L

G

G

D

I

R

L

L

A

A

E

A

S

F

L

G

G

V

M

D

Y

V

V

T

Y

G

F

V

Y

T
x
D

R

I

S

E

G

N

R

K

D

L

-

P

-

L

G

G

T

N

L

A

T

T

P

Q

G

V

Q

Q

-

H

W

L

R

S

E

D

R

L

L

L

G

N

D

M

Y

S

D

D

W

V

L

V

V

S

L

L

A

H

A

V

P

P

S

E

T

N

G

P

D

S

T

T

H

K

A

N

L

M

I

M

G

G

T

A

A

K

E

E

L

I

A

S

A

L

M

M

K

K

P

P

G

G

A

S

W

L

L

L

V

I

N

N

V
x
A

A
x
S

R
|
R

G

G

D

T

M

V

V

V

D

D

Q

I

P

P

A

A

L

L

I

C

E

D

A

A

L

L

E

A

K

S

R

K

R

H

I

L

A

A

G

G

A

A

F

A

L

I

D
|
D

V

V

V

F

D

P

P

T

E

E

P

P

L

A

P

T

D

N

D

S

H

D

P

P

L

-

W

F

T

T

T

S

P

P

-

L

-

C

-

E

-

F

-

D

N

N

V

V

I

L

H

L

S

T

M

P

H
|
H

L
x
I

S
x
G

G
|
G

R

S

S

T

Q

Q

HI 161:162 (≠ TI 147:148) binding NAD(+)
D181 (≠ T167) binding NAD(+)
ASR 238:240 (≠ VAR 221:223) binding NAD(+)
D264 (= D247) binding NAD(+)
HIGG 292:295 (≠ HLSG 271:274) binding NAD(+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1psdA The allosteric ligand site in the vmax-type cooperative enzyme phosphoglycerate dehydrogenase (see paper)
29% identity, 74% coverage: 48:277/311 of query aligns to 56:292/404 of 1psdA

query
sites
1psdA

R

R

P

T

D

H

W

L

T

T

G

E

E

D

A

V

A

I

A

N

A

A

G

A

V

E

R

K

L

L

K

V

W

A

L

I

S

G

T

C

I

F

Y

C

A

I

G

G

I

T

D

N

A

Q

F

V

D

D

V

L

D

D

L

A

K

A

T

K

R

R

G

G

T

I

I

P

L

V

T

F

N

N

G

A

V

P

G

F

I

S

N
|
N

A

T

I

R

A

S

V

V

A

A

E

E

Y

L

A

V

V

I

M

G

G

E

M

L

L

L

A

L

K

R

R

G

F

V

D

P

Q

E

V

A

V

N

R

A

M

K

A

A

D

H

R

R

R

G

E

V

W

W

P

N

A

K

D

L

A

A

P

A

G

G

K

S

V

F

E

E

L

A

F

R

E

G

T

K

R

K

A

L

L

G

I

I

V

I

G

G

Y

Y

G

G

T
x
H

I
|
I

G

G

R

T

M

Q

I

L

G

G

D

I

R

L

L

A

A

E

A

S

F

L

G

G

V

M

D

Y

V

V

T

Y

G

F

V

Y

T
x
D

R
x
I

S

E

G

N

R
x
K

D

L

-

P

-

L

G

G

T

N

L

A

T

T

P

Q

G

V

Q

Q

-

H

W

L

R

S

E

D

R

L

L

L

G

N

D

M

Y

S

D

D

W

V

L

V

V

S

L

L

A
x
H

A
x
V

P
|
P

S

E

T

N

G

P

D

S

T

T

H

K

A

N

L

M

I

M

G

G

T

A

A

K

E

E

L

I

A

S

A

L

M

M

K

K

P

P

G

G

A

S

W

L

L

L

V

I

N

N

V
x
A

A
x
S

R
|
R

G

G

D

T

M

V

V

V

D

D

Q

I

P

P

A

A

L

L

I

C

E

D

A

A

L

L

E

A

K

S

R

K

R

H

I

L

A

A

G

G

A

A

F

A

L

I

D
|
D

V

V

V

F

D

P

P

T

E
|
E

P

P

L

A

P

T

D

N

D

S

H

D

P

P

L

-

W

F

T

T

T

S

P

P

-

L

-

C

-

E

-

F

-

D

N

N

V

V

I

L

H

L

S

T

M

P

H
|
H

L

I

S

G

G

G

R

S

S

T

Q

Q

active site: N102 (= N94), R234 (= R223), D258 (= D247), E263 (= E252), H286 (= H271)
binding nicotinamide-adenine-dinucleotide: N102 (= N94), H155 (≠ T147), I156 (= I148), D175 (≠ T167), I176 (≠ R168), K179 (≠ R171), H204 (≠ A193), V205 (≠ A194), P206 (= P195), A232 (≠ V221), S233 (≠ A222), R234 (= R223), H286 (= H271)

Sites not aligning to the query:

binding serine: 338, 340, 341, 344

1ybaA The active form of phosphoglycerate dehydrogenase (see paper)
29% identity, 74% coverage: 48:277/311 of query aligns to 58:294/406 of 1ybaA

query
sites
1ybaA

R

R

P

T

D

H

W

L

T

T

G

E

E

D

A

V

A

I

A

N

A

A

G

A

V

E

R

K

L

L

K

V

W

A

L

I

S

G

T

C

I

F

Y
x
C

A
x
I

G
|
G

I

T

D
x
N

A

Q

F

V

D

D

V

L

D

D

L

A

K

A

T

K

R

R

G

G

T

I

I

P

L

V

T

F

N

N

G

A

V

P

G
x
F

I

S

N
|
N

A

T

I

R

A

S

V
|
V

A

A

E

E

Y

L

A

V

V

I

M

G

G

E

M

L

L

L

A

L

K

R

R

G

F

V

D

P

Q

E

V

A

V

N

R

A

M

K

A

A

D

H

R

R

R

G

E

V

W

W

P

N

A

K

D

L

A

A

P

A

G

G

K

S

V

F

E

E

L

A

F

R

E

G

T

K

R

K

A

L

L

G

I

I

V

I

G
|
G

Y

Y

G
|
G

T
x
H

I
|
I

G

G

R

T

M

Q

I

L

G

G

D

I

R

L

L

A

A

E

A

S

F

L

G

G

V

M

D

Y

V

V

T

Y

G

F

V
x
Y

T
x
D

R
x
I

S

E

G

N

R
x
K

D

L

-

P

-

L

G

G

T

N

L

A

T

T

P

Q

G

V

Q

Q

-

H

W

L

R

S

E

D

R

L

L

L

G

N

D

M

Y

S

D

D

W

V

L

V

V

S

L

L

A
x
H

A
x
V

P
|
P

S

E

T

N

G

P

D

S

T

T

H

K

A

N

L

M

I

M

G

G

T

A

A

K

E

E

L

I

A

S

A

L

M

M

K

K

P

P

G

G

A

S

W

L

L

L

V

I

N

N

V
x
A

A
x
S

R
|
R

G

G

D

T

M

V

V

V

D

D

Q

I

P

P

A

A

L

L

I

C

E

D

A

A

L

L

E

A

K

S

R

K

R

H

I

L

A

A

G

G

A

A

F

A

L

I

D
|
D

V

V

V

F

D

P

P

T

E
|
E

P

P

L

A

P

T

D

N

D

S

H

D

P

P

L

-

W

F

T

T

T

S

P

P

-

L

-

C

-

E

-

F

-

D

N

N

V

V

I

L

H

L

S

T

M

P

H
|
H

L

I

S
x
G

G

G

R

S

S

T

Q

Q

active site: N104 (= N94), R236 (= R223), D260 (= D247), E265 (= E252), H288 (= H271)
binding 2-oxoglutaric acid: C79 (≠ Y69), I80 (≠ A70)
binding nicotinamide-adenine-dinucleotide: I80 (≠ A70), F102 (≠ G92), V108 (= V98), G154 (= G144), G156 (= G146), H157 (≠ T147), I158 (= I148), Y176 (≠ V166), D177 (≠ T167), I178 (≠ R168), K181 (≠ R171), H206 (≠ A193), V207 (≠ A194), P208 (= P195), A234 (≠ V221), S235 (≠ A222), R236 (= R223), H288 (= H271), G290 (≠ S273)
binding phosphate ion: G81 (= G71), N83 (≠ D73)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 56, 57

6jujA Crystal structure of formate dehydrogenase mutant v198i/c256i/p260s/e261p/s381n/s383f from pseudomonas sp. 101in complex with non-natural cofactor nicotinamide cytosine dinucleotide (see paper)
30% identity, 86% coverage: 36:303/311 of query aligns to 87:363/381 of 6jujA

query
sites
6jujA

V

L

A

V

D

D

A

A

D

D

I

V

A

V

W

-

V

-

D

-

M

I

M

S

R

Q

P

P

D
x
F

W

W

-

P

-

A

-

Y

-

L

T

T

G

P

E

E

A

R

A

I

A

A

A

K

G

A

V

K

R

N

L

L

K

K

W

L

L

A

S

L

T

T

I

A

Y

G

A

I

G

G

I

S

D

D

A

H

F

V

D

D

V

L

D

Q

L

S

L

A

K

I

T

D

R

R

G

N

T

V

I

T

L

V

T

A

N

E

G

V

V

T

G

Y

I

C

N
|
N

A

S

I

I

A

S

V
|
V

A

A

E

E

Y

H

A

V

V

V

M

M

G

M

M

I

L

L

A

S

A

L

A

V

K

R

R

N

F

Y

D

L

Q

P

V

S

V

H

R

E

M

W

A

A

D

R

R

K

R

G

E

G

W

W

P

N

A

I

D

-

A

A

P

D

G

C

K

V

V

S

E

H

L

A

F

Y

E

D

T

L

R

E

A

A

L

M

I

H

V

V

G

G

Y

T

-

I

-

A

G
|
G

T

R

I
|
I

G

G

R

L

M

A

I

V

G

L

D

R

R

R

L

L

A

A

A

P

F

F

G

D

V

V

D

H

V

L

T

H

G

Y

V

T

T
x
D

R
|
R

S

H

-

R

-

L

-

P

-

E

-

S

-

V

G

E

R

K

D

E

G

L

T

N

L

L

T

T

P

W

G

H

Q

A

W

T

R

R

E

E

R

D

L

M

G

Y

D

P

-

V

Y

C

D

D

W

V

L

V

V

T

L

L

A

N

A
x
I

P
|
P

S

L

T
x
H

G

S

D

P

T

T

H

E

A

H

L

M

I

I

G

N

T

D

A

E

E

T

L

L

A

K

A

L

M

F

K

K

P

R

G

G

A

A

W

Y

L

I

V

V

N

N

V
x
T

A
|
A

R

R

G

G

D

K

M

L

V

C

D

D

Q

R

P

D

A

A

L

V

I

A

E

R

A

A

L

L

E

E

K

S

R

G

R

R

I

L

A

A

G

G

A

Y

F

A

L

G

D
|
D

V

V

V

W

D

F

P

P

E

Q

P

P

L

A

P

P

D

K

D

D

H

H

P

P

L

W

W

R

T

T

T

M

P

P

N

Y

V

N

I

G

H

M

S

T

M

P

H
|
H

L

I

S
|
S

G
|
G

R

T

S

T

Q

L

A

T

K

A

M

Q

F

-

M

A

R

R

A

Y

A

A

L

G

F

T

L

R

E

E

N

I

A

L

R

E

A

C

F

F

A

F

E

E

G

G

R

R

P

P

M

I

K

R

N

D

binding [[(2S,3S,4R,5S)-5-(3-aminocarbonylpyridin-1-ium-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2S,3S,4R,5S)-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate: F98 (≠ D50), N146 (= N94), V150 (= V98), G200 (= G146), I202 (= I148), D221 (≠ T167), R222 (= R168), I255 (≠ A194), P256 (= P195), H258 (≠ T197), T282 (≠ V221), A283 (= A222), D308 (= D247), H332 (= H271), S334 (= S273), G335 (= G274)

3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
30% identity, 93% coverage: 21:310/311 of query aligns to 22:315/526 of 3dc2A

query
sites
3dc2A

I

V

E

E

P

V

H

R

W

W

F

V

-

D

-

G

A

P

S

D

R

R

E

D

E

K

A

L

I

L

A

A

M

A

V

V

A

P

D

E

A

A

D

D

I

A

A

L

W

L

V

V

D

-

M

-

R

R

P

S

D

A

W

T

T

T

-

V

-

D

G

A

E

E

A

V

A

L

A

A

G

A

V

P

R

K

L

L

K

K

W

I

L

V

S

A

T

R

I

A

Y

G

A

V

G

G

I

L

D

D

A

N

F

V

D

D

V

V

D

D

L

A

K

T

T

A

R

R

G

G

T

V

I

L

L

V

T

V

N

N

G

A

V

P

G

T

I

S

N
|
N

A

I

I

H

A

S

V

A

A

A

E

E

Y

H

A

A

V

L

M

A

G

L

M

L

L

L

A

A

A

S

K

R

R

Q

F

I

D

P

Q

A

V

A

V

D

R

A

M

S

A

L

D

R

R

E

R

H

E

T

W

W

P

K

A

R

D

S

A

S

P

F

G

S

K

G

V

T

E

E

L

I

F

F

E

G

T

K

R

T

A

V

L

G

I

V

V

V

G

G

Y

L

G

G

T

R

I

I

G

G

R

Q

M

L

I

V

G

A

D

Q

R

R

L

I

A

A

F

F

G

G

V

A

D

Y

V

V

T

V

G

-

V

-

T

-

R

-

S

-

G

A

R

Y

D

D

G

P

T

Y

L

V

T

S

P

P

G

A

Q

R

-

A

-

Q

-

L

-

G

-

I

-

E

-

L

-

S

W

L

R

D

E

D

R

L

L

L

G

A

D

R

Y

A

D

D

W

F

L

I

V

S

L

V

A

H

A

L

P

P

S

K

T

T

G

P

D

E

T

T

H

A

A

G

L

L

I

I

G

D

T

K

A

E

E

A

L

L

A

A

A

K

M

T

K

K

P

P

G

G

A

V

W

I

L

I

V

V

N

N

V

A

A

A

R
|
R

G

G

D

G

M

L

V

V

D

D

Q

E

P

A

A

A

L

L

I

A

E

D

A

A

L

I

E

T

K

G

R

G

R

H

I

V

A

R

G

A

A

A

F

G

L

L

D
|
D

V

V

V

F

D

A

P

T

E
|
E

P

P

L

C

P

T

D

-

D

D

H

S

P

P

L

L

W

F

T

E

T

L

P

A

N

Q

V

V

I

V

H

V

S

T

M

P

H
|
H

L

L

S

-

G

G

R

A

S

S

Q

T

A

A

K

E

M

A

F

Q

M

D

R

R

A

A

A

G

A

T

L

D

F

V

L

A

E

E

N

S

A

V

R

R

A

L

F

A

A

L

E

A

G

G

R

E

P

F

M

V

K

P

N

D

V

A

V

V

D

N

L

V

D

G

A

G

G

G

active site: N96 (= N94), R230 (= R223), D254 (= D247), E259 (= E252), H277 (= H271)

Sites not aligning to the query:

binding serine: 458, 460, 461, 462, 463, 464, 465, 484

3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
30% identity, 93% coverage: 21:310/311 of query aligns to 23:316/525 of 3ddnB

query
sites
3ddnB

I

V

E

E

P

V

H

R

W

W

F

V

-

D

-

G

A

P

S

D

R

R

E

D

E

K

A

L

I

L

A

A

M

A

V

V

A

P

D

E

A

A

D

D

I

A

A

L

W

L

V

V

D

-

M

-

R

R

P

S

D

A

W

T

T

T

-

V

-

D

G

A

E

E

A

V

A

L

A

A

G

A

V

P

R

K

L

L

K

K

W

I

L

V

S

A

T
x
R

I

A

Y

G

A
x
V

G

G

I

L

D

D

A

N

F

V

D

D

V

V

D

D

L

A

K

T

T

A

R

R

G

G

T

V

I

L

L

V

T

V

N

N

G

A

V

P

G

T

I

S

N
|
N

A

I

I

H

A

S

V

A

A

A

E

E

Y

H

A

A

V

L

M

A

G

L

M

L

L

L

A

A

A

S

K

R

R

Q

F

I

D

P

Q

A

V

A

V

D

R

A

M

S

A

L

D

R

R

E

R

H

E

T

W

W

P

K

A

R

D

S

A

S

P

F

G

S

K

G

V

T

E

E

L

I

F

F

E

G

T

K

R

T

A

V

L

G

I

V

V

V

G

G

Y

L

G

G

T

R

I

I

G

G

R

Q

M

L

I

V

G

A

D

Q

R

R

L

I

A

A

F

F

G

G

V

A

D

Y

V

V

T

V

G

-

V

-

T

-

R

-

S

-

G

A

R

Y

D

D

G

P

T

Y

L

V

T

S

P

P

G

A

Q

R

-

A

-

Q

-

L

-

G

-

I

-

E

-

L

-

S

W

L

R

D

E

D

R

L

L

L

G

A

D

R

Y

A

D

D

W

F

L

I

V

S

L

V

A

H

A

L

P

P

S

K

T

T

G

P

D

E

T

T

H

A

A

G

L

L

I

I

G

D

T

K

A

E

E

A

L

L

A

A

A

K

M

T

K

K

P

P

G

G

A

V

W

I

L

I

V

V

N

N

V

A

A

A

R
|
R

G

G

D

G

M

L

V

V

D

D

Q

E

P

A

A

A

L

L

I

A

E

D

A

A

L

I

E

T

K

G

R

G

R

H

I

V

A

R

G

A

A

A

F

G

L

L

D
|
D

V

V

V

F

D

A

P

T

E
|
E

P

P

L

C

P

T

D

-

D

D

H

S

P

P

L

L

W

F

T

E

T

L

P

A

N

Q

V

V

I

V

H

V

S

T

M

P

H
|
H

L

L

S

-

G

G

R
x
A

S

S

Q

T

A

A

K

E

M

A

F
x
Q

M

D

R

R

A

A

A

G

A

T

L

D

F

V

L

A

E

E

N

S

A

V

R

R

A

L

F

A

A

L

E

A

G

G

R

E

P

F

M

V

K

P

N

D

V

A

V

V

D

N

L

V

D

G

A

G

G

G

active site: N97 (= N94), R231 (= R223), D255 (= D247), E260 (= E252), H278 (= H271)
binding 2-oxo-3-(phosphonooxy)propanoic acid: R70 (≠ T67), V73 (≠ A70), N97 (= N94), A281 (≠ R275), Q287 (≠ F281)

7ya4A Formate dehydrogenase from novosphingobium sp. Ap12 with NAD and azide
29% identity, 77% coverage: 71:310/311 of query aligns to 123:371/384 of 7ya4A

query
sites
7ya4A

G

G

I

S

D

D

A

H

F

V

D

D

V

L

D

E

L

S

L

A

K

I

T

K

R

H

G

G

T

V

I

T

L

V

T

A

N

E

G

V

V

T

G

G

I

S

N
|
N

A
x
S

I

I

A

S

V
|
V

A

S

E

E

Y

H

A

A

V

V

M

M

G

M

M

I

L

L

A

S

A

L

A

V

K

R

R

N

F

Y

D

I

Q

P

V

A

V

H

R

E

M

W

A

A

D

E

R

K

R

G

E

G

W

W

P

N

-

I

A

A

D

D

A

C

P

V

G

E

K

R

V

S

E

-

L

-

F

Y

E

D

T

V

R

E

A

G

L

M

I

H

V

V

G

G

Y

T

-

V

-
x
A

-

A

G
|
G

T
x
R

I
|
I

G

G

R

L

M

A

I

I

G

L

D

K

R

R

L

M

A

K

A

P

F

F

G

D

V

V

D

H

V

L

T

H

G

Y

V
x
Y

T

A

R
|
R

-

H

-

R

-

L

S

S

G

K

R

E

D

E

G

E

T

E

L

E

T

L

P

G

G

L

Q

T

W

F

R

H

E

E

R

N

L

V

G

E

D

D

-

M

-

V

-

K

-

V

Y

C

D

D

W

V

L

V

V

T

L

I

A

N

A

A

P
|
P

S

L

T
x
H

G

P

D
x
E

T
|
T

H

H

A

H

L

M

I

F

G

D

T

E

A

A

E

M

L

I

A

K

M

M

K

K

P

R

G

G

A

A

W

Y

L

I

V

V

N

N

V
x
T

A
|
A

R
|
R

G

A

D

E

M

I

V

C

D

D

Q

R

P

D

A

A

L

I

I

V

E

R

A

A

L

V

E

E

K

S

R

G

R

H

I

L

A

A

G

G

A

Y

F

A

L

G

D
|
D

V

V

V

W

D

N

P

P

E

Q

P

P

L

A

P

P

D

A

D

D

H

H

P

P

L

W

W

R

T

T

T

M

P

P

N

W

V

N

I

G

H

M

S

T

M

P

H
|
H

L

M

S
|
S

G
|
G

R

T

S

S

Q

L

A

S

K

G

M

Q

F

-

M

A

R

R

A

Y

A

T

A

A

L

G

F

T

L

R

E

E

N

I

A

L

R

E

A

C

F

W

A

F

E

E

G

G

R

R

P

P

M

I

K

R

-

E

N

D

V

Y

V

V

D

I

L

V

D

D

A

G

G

G

binding azide ion: R284 (= R223), H332 (= H271)
binding nicotinamide-adenine-dinucleotide: N146 (= N94), S147 (≠ A95), V150 (= V98), A198 (vs. gap), G200 (= G146), R201 (≠ T147), I202 (= I148), Y220 (≠ V166), R222 (= R168), P256 (= P195), H258 (≠ T197), E260 (≠ D199), T261 (= T200), T282 (≠ V221), A283 (= A222), R284 (= R223), D308 (= D247), H332 (= H271), S334 (= S273), G335 (= G274)

Sites not aligning to the query:

Query Sequence

>Ga0059261_0925 FitnessBrowser__Korea:Ga0059261_0925
MKAVLPALARPLIEPGLPAGIEPHWFASREEAIAMVADADIAWVDMMRPDWTGEAAAAGV
RLKWLSTIYAGIDAFDVDLLKTRGTILTNGVGINAIAVAEYAVMGMLAAAKRFDQVVRMA
DRREWPADAPGKVELFETRALIVGYGTIGRMIGDRLAAFGVDVTGVTRSGRDGTLTPGQW
RERLGDYDWLVLAAPSTGDTHALIGTAELAAMKPGAWLVNVARGDMVDQPALIEALEKRR
IAGAFLDVVDPEPLPDDHPLWTTPNVIHSMHLSGRSQAKMFMRAAALFLENARAFAEGRP
MKNVVDLDAGY

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory