SitesBLAST
Comparing Ga0059261_0929 FitnessBrowser__Korea:Ga0059261_0929 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1zxyA Anthranilate phosphoribosyltransferase from sulfolobus solfataricus in complex with prpp and magnesium (see paper)
40% identity, 89% coverage: 26:319/331 of query aligns to 28:320/344 of 1zxyA
- binding magnesium ion: D223 (= D223), E224 (= E224)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: A78 (≠ C77), G79 (= G78), D83 (= D82), T87 (= T86), N89 (= N88), S91 (= S90), T92 (= T91), K106 (= K106), S118 (≠ A118), D223 (= D223), E224 (= E224)
2gvqD Anthranilate phosphoribosyl-transferase (trpd) from s. Solfataricus in complex with anthranilate (see paper)
40% identity, 89% coverage: 26:319/331 of query aligns to 28:320/345 of 2gvqD
P50384 Anthranilate phosphoribosyltransferase; EC 2.4.2.18 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see 2 papers)
40% identity, 89% coverage: 26:319/331 of query aligns to 28:320/345 of P50384
- T87 (= T86) binding
- K106 (= K106) mutation to Q: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency dedreases only 10-fold.
- H107 (= H107) mutation to A: Limited effect on either affinity for anthranilate and catalytic efficiency. 300-fold decrease of the affinity for anthranilate, whereas catalytic efficiency remains nearly unchanged; when associated with A-178.
- S118 (≠ A118) binding
- H154 (= H154) mutation to A: Limited effect on either affinity for anthranilate and catalytic efficiency.
- R164 (= R164) mutation to A: Strong decrease of the affinity for anthranilate, although only a moderate 7-fold decrease in catalytic efficiency.
- D223 (= D223) mutation to N: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency is unchanged.
- E224 (= E224) mutation to Q: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency is unchanged.
1gxbA Anthranilate phosphoribosyltransferase in complex with pyrophosphate and magnesium (see paper)
38% identity, 89% coverage: 26:319/331 of query aligns to 28:316/339 of 1gxbA
5nofA Anthranilate phosphoribosyltransferase from thermococcus kodakaraensis (see paper)
40% identity, 87% coverage: 36:323/331 of query aligns to 33:316/325 of 5nofA
1kgzB Crystal structure analysis of the anthranilate phosphoribosyltransferase from erwinia carotovora (current name, pectobacterium carotovorum) (see paper)
39% identity, 93% coverage: 14:320/331 of query aligns to 15:320/330 of 1kgzB
- binding manganese (ii) ion: S92 (= S90), D222 (= D223), E223 (= E224), E223 (= E224)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V79 (≠ C77), G82 (= G80), G83 (= G81), N90 (= N88), I91 (≠ V89), S92 (= S90), T93 (= T91), K108 (= K106), S118 (≠ A118)
3gbrA Anthranilate phosphoribosyl-transferase (trpd) double mutant d83g f149s from s. Solfataricus (see paper)
39% identity, 89% coverage: 26:319/331 of query aligns to 28:317/341 of 3gbrA
- binding manganese (ii) ion: S91 (= S90), D220 (= D223), E221 (= E224), E221 (= E224)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: A78 (≠ C77), G79 (= G78), T80 (= T79), G81 (= G80), N89 (= N88), V90 (= V89), S91 (= S90), T92 (= T91), K106 (= K106), G114 (= G117)
5c1rA Stereoisomer of prpp bound in the active site of mycobacterium tuberculosis anthranilate phosphoribosyl (anprt; trpd)
44% identity, 95% coverage: 14:328/331 of query aligns to 16:338/343 of 5c1rA
- active site: V82 (≠ C77)
- binding 5-O-[(R)-hydroxy(phosphonooxy)phosphoryl]-1-O-phosphono-alpha-D-ribofuranose: V82 (≠ C77), G83 (= G78), G86 (= G81), N93 (= N88), S95 (= S90), T96 (= T91), K111 (= K106), R115 (= R110), A117 (= A112), S118 (= S113), S119 (= S114)
- binding magnesium ion: D87 (= D82), V89 (≠ H84), S95 (= S90), E228 (= E224)
3r88B Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs145) (see paper)
44% identity, 95% coverage: 14:328/331 of query aligns to 16:338/344 of 3r88B
- active site: V82 (≠ C77)
- binding 2-amino-4,5-dimethoxybenzoic acid: N114 (= N109), A155 (= A150), P156 (≠ A151), H159 (= H154), Y162 (≠ M157), R163 (≠ G158), A166 (= A161)
- binding magnesium ion: S95 (= S90), D227 (= D223), E228 (= E224), E228 (= E224)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V82 (≠ C77), G83 (= G78), G85 (= G80), G86 (= G81), N93 (= N88), S95 (= S90), T96 (= T91), K111 (= K106), N114 (= N109), A117 (= A112), S118 (= S113), S119 (= S114)
4zokA Methylsulfonyl-containing inhibitor bound in the substrate capture site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
44% identity, 95% coverage: 14:328/331 of query aligns to 16:337/342 of 4zokA
- active site: V82 (≠ C77)
- binding 4-methyl-2-{[2-methyl-6-(methylsulfonyl)phenyl]amino}benzoic acid: P156 (≠ A151), R163 (≠ G158), A166 (= A161), A167 (≠ P162)
- binding magnesium ion: S95 (= S90), E228 (= E224)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G86 (= G81), N93 (= N88), S95 (= S90), T96 (= T91), K111 (= K106), A116 (= A111), A117 (= A112), S119 (= S114), G122 (= G117)
4zofA Lobenzarit-like inhibitor bound in the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
44% identity, 95% coverage: 14:328/331 of query aligns to 16:337/342 of 4zofA
- active site: V82 (≠ C77)
- binding 2-[(2-carboxy-5-nitrophenyl)amino]-3-methylbenzoic acid: G113 (= G108), N114 (= N109), A155 (= A150), H159 (= H154), Y162 (≠ M157), R169 (= R164), G182 (= G177)
- binding magnesium ion: S95 (= S90), D227 (= D223), E228 (= E224), E228 (= E224)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G78), G85 (= G80), G86 (= G81), N93 (= N88), S95 (= S90), T96 (= T91), K111 (= K106), A116 (= A111), A117 (= A112), S119 (= S114), G122 (= G117)
5c7sA Prpp complexed with two mn2+ in the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
44% identity, 95% coverage: 14:328/331 of query aligns to 17:339/344 of 5c7sA
- active site: V83 (≠ C77)
- binding manganese (ii) ion: G84 (= G78), S96 (= S90), D228 (= D223), E229 (= E224), E229 (= E224)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V83 (≠ C77), G84 (= G78), G86 (= G80), G87 (= G81), N94 (= N88), S96 (= S90), T97 (= T91), K112 (= K106), N115 (= N109), S120 (= S114)
4zojA Methylsulfanyl-containing inhibitor bound in the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
44% identity, 95% coverage: 14:328/331 of query aligns to 16:336/341 of 4zojA
- active site: V82 (≠ C77)
- binding 4-methyl-2-{[2-methyl-6-(methylsulfanyl)phenyl]amino}benzoic acid: N114 (= N109), A155 (= A150), P156 (≠ A151), H159 (= H154), Y162 (≠ M157), R169 (= R164), G182 (= G177)
- binding magnesium ion: S95 (= S90), D227 (= D223), E228 (= E224), E228 (= E224)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G78), G85 (= G80), G86 (= G81), N93 (= N88), S95 (= S90), T96 (= T91), K111 (= K106), R115 (= R110), A116 (= A111), A117 (= A112), S119 (= S114)
4owoA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 6-fluoroanthranilate, prpp and magnesium (see paper)
44% identity, 95% coverage: 14:328/331 of query aligns to 18:342/348 of 4owoA
- active site: V84 (≠ C77)
- binding 2-azanyl-6-fluoranyl-benzoic acid: V84 (≠ C77), G85 (= G78), T86 (= T79), H114 (= H107), N116 (= N109), N116 (= N109), A157 (= A150), P158 (≠ A151), H161 (= H154), Y164 (≠ M157), A168 (= A161), R171 (= R164), G184 (= G177)
- binding magnesium ion: S97 (= S90), D229 (= D223), E230 (= E224), E230 (= E224)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V84 (≠ C77), G85 (= G78), G87 (= G80), G88 (= G81), N95 (= N88), S97 (= S90), T98 (= T91), K113 (= K106), A118 (= A111), A119 (= A112), S120 (= S113), S121 (= S114), G124 (= G117)
5c2lA Magnesium soaked into the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
44% identity, 95% coverage: 14:328/331 of query aligns to 18:342/349 of 5c2lA
P9WFX5 Anthranilate phosphoribosyltransferase; EC 2.4.2.18 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
44% identity, 95% coverage: 14:328/331 of query aligns to 40:364/370 of P9WFX5
- G107 (= G78) binding
- T115 (= T86) binding
- G147 (≠ A118) binding
4n93A Alternative substrates of mycobacterium tuberculosis anthranilate phosphoribosyl transferase (see paper)
43% identity, 95% coverage: 14:328/331 of query aligns to 17:340/346 of 4n93A
- active site: V83 (≠ C77)
- binding 2-amino-6-methylbenzoic acid: V83 (≠ C77), G84 (= G78), T85 (= T79), H113 (= H107), N115 (= N109), N115 (= N109), A156 (= A150), P157 (≠ A151), H160 (= H154), Y163 (≠ M157), A167 (= A161), R170 (= R164), G183 (= G177), P184 (= P178)
- binding magnesium ion: S96 (= S90), D228 (= D223), E229 (= E224), E229 (= E224)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G84 (= G78), G86 (= G80), G87 (= G81), N94 (= N88), S96 (= S90), T97 (= T91), K112 (= K106), A117 (= A111), A118 (= A112), S120 (= S114), G123 (= G117)
4n5vA Alternative substrates of mycobacterium tuberculosis anthranilate phosphoribosyl transferase (see paper)
43% identity, 95% coverage: 14:328/331 of query aligns to 18:341/347 of 4n5vA
- active site: V84 (≠ C77)
- binding 2-amino-4-fluorobenzoic acid: V84 (≠ C77), G85 (= G78), H114 (= H107), G115 (= G108), N116 (= N109), A157 (= A150), A157 (= A150), P158 (≠ A151), H161 (= H154), Y164 (≠ M157), R165 (≠ G158), A168 (= A161), R171 (= R164), G184 (= G177)
- binding magnesium ion: S97 (= S90), D229 (= D223), E230 (= E224), E230 (= E224)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G85 (= G78), G87 (= G80), G88 (= G81), N95 (= N88), S97 (= S90), T98 (= T91), K113 (= K106), A119 (= A112), S120 (= S113), S121 (= S114), G124 (= G117)
4owuA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 5-methylanthranilate, prpp and magnesium (see paper)
44% identity, 95% coverage: 14:328/331 of query aligns to 17:341/347 of 4owuA
- active site: V83 (≠ C77)
- binding 2-azanyl-5-methyl-benzoic acid: N115 (= N109), P157 (≠ A151), Y163 (≠ M157), R164 (≠ G158), A167 (= A161)
- binding magnesium ion: S96 (= S90), D228 (= D223), E229 (= E224), E229 (= E224)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G84 (= G78), G86 (= G80), G87 (= G81), N94 (= N88), S96 (= S90), T97 (= T91), K112 (= K106), A118 (= A112), S120 (= S114), G123 (= G117)
4owqA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 3-methylanthranilate, prpp and magnesium (see paper)
44% identity, 95% coverage: 14:328/331 of query aligns to 17:341/347 of 4owqA
- active site: V83 (≠ C77)
- binding 2-azanyl-3-methyl-benzoic acid: P157 (≠ A151), H160 (= H154), Y163 (≠ M157), A167 (= A161), R171 (= R165)
- binding magnesium ion: S96 (= S90), D228 (= D223), E229 (= E224), E229 (= E224)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V83 (≠ C77), G84 (= G78), G86 (= G80), G87 (= G81), N94 (= N88), S96 (= S90), T97 (= T91), K112 (= K106), A117 (= A111), A118 (= A112), S120 (= S114), G123 (= G117)
Query Sequence
>Ga0059261_0929 FitnessBrowser__Korea:Ga0059261_0929
VTRFSLLPDPATPLAREAAAQAFADILDGSAPEPEIEAFLIALSERGETSIEIAEAARAM
RQRVIPVSAPANAIDVCGTGGDGHHTLNVSTAVAIVVAAAGVPVAKHGNRAASSKSGAAD
TLEALGLDLDRAAANAEAHLNDLGIAFLFAAKHHPAMGRIAPLRRRIARRTIFNLMGPLA
NPAGVQRQLIGIARPDYAPIYAEALEQLGVKGAAIVAGEEGLDELSPEGGSLVVSIGDVS
LPVRVTPADAGLPSHPLVSLRGGDARENAAALRRLLLGERGPYRDAVLFNSAAALVVAGE
AVDLREGAEEAAETIDKGLANALLDCWIAAA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory