SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ga0059261_1479 Ga0059261_1479 Lactate dehydrogenase and related dehydrogenases to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
41% identity, 96% coverage: 9:327/332 of query aligns to 2:321/334 of 5aovA

query
sites
5aovA

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active site: L100 (= L108), R241 (= R247), D265 (= D271), E270 (= E276), H288 (= H294)
binding glyoxylic acid: M52 (≠ T59), L53 (≠ V60), L53 (≠ V60), Y74 (≠ F82), A75 (≠ G83), A75 (≠ G83), V76 (≠ S84), G77 (= G85), R241 (= R247), H288 (= H294)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ S84), G77 (= G85), L100 (= L108), T104 (= T112), G157 (= G161), F158 (≠ M162), G159 (= G163), R160 (= R164), I161 (= I165), Y179 (≠ H183), S180 (≠ N184), R181 (= R185), T182 (≠ H186), K184 (≠ L188), A211 (≠ H217), V212 (≠ T218), P213 (= P219), T218 (≠ S224), I239 (≠ A245), A240 (≠ S246), R241 (= R247), D265 (= D271), H288 (= H294), G290 (= G296)

6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
42% identity, 96% coverage: 9:327/332 of query aligns to 1:320/332 of 6biiA

query
sites
6biiA

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active site: L99 (= L108), R240 (= R247), D264 (= D271), E269 (= E276), H287 (= H294)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (≠ S84), G76 (= G85), L99 (= L108), T103 (= T112), G156 (= G161), F157 (≠ M162), G158 (= G163), R159 (= R164), I160 (= I165), A179 (≠ N184), R180 (= R185), S181 (≠ H186), K183 (≠ L188), A210 (≠ H217), V211 (≠ T218), P212 (= P219), E216 (= E223), T217 (≠ S224), V238 (≠ A245), A239 (≠ S246), R240 (= R247), D264 (= D271), H287 (= H294), G289 (= G296)

2dbqA Crystal structure of glyoxylate reductase (ph0597) from pyrococcus horikoshii ot3, complexed with NADP (i41) (see paper)
40% identity, 96% coverage: 9:327/332 of query aligns to 2:321/333 of 2dbqA

query
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2dbqA

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active site: L100 (= L108), R241 (= R247), D265 (= D271), E270 (= E276), H288 (= H294)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ S84), G77 (= G85), L100 (= L108), T104 (= T112), G157 (= G161), L158 (≠ M162), G159 (= G163), R160 (= R164), I161 (= I165), Y179 (≠ H183), S180 (≠ N184), R181 (= R185), T182 (≠ H186), K184 (≠ L188), A211 (≠ H217), V212 (≠ T218), P213 (= P219), T218 (≠ S224), I239 (≠ A245), A240 (≠ S246), R241 (= R247), D265 (= D271), H288 (= H294), G290 (= G296)

O58320 Glyoxylate reductase; EC 1.1.1.26 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
40% identity, 96% coverage: 9:327/332 of query aligns to 2:321/334 of O58320

query
sites
O58320

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F

F

G

N

L

M

S

R

I

I

H

L

Y

Y

H

Y

N
x
S

R
|
R

H
x
T

R

R

L

K

P

E

E

E

V

V

V

-

E

E

A

R

E

E

L

L

G

N

A

A

A

E

W

F

H

K

G

P

D

-

L

L

D

E

A

D

M

L

L

L

R

R

E

E

I

S

D

D

I

F

L

V

T

V

I

L

H

A

T

V

P

P

L

L

N

T

E

R

E

E

S

T

R

Y

D

H

L

L

I

I

D

N

A

E

R

E

R

R

I

L

G

K

L

L

L

M

G

K

P

K

Q

T

V

A

Y

I

L

L

I

I

N

N

A
x
I

S
x
A

R
|
R

G

G

G

K

I

V

V

V

D

D

E

T

E

N

A

A

M

L

V

V

D

K

A

A

L

L

E

K

A

E

G

G

R

W

L

I

A

A

G

G

A

A

G

G

L

L

D

D

V

V

W

F

R

E

F

E

E

E

P

P

Q

Y

I

Y

D

N

P

E

R

E

L

L

L

F

A

K

L

L

P

D

N

N

V

V

M

L

T

T

P

P

H
|
H

M
x
I

G
|
G

S

S

A

A

T

S

Y

F

E

G

G

A

R

R

H

E

A

G

T

M

G

A

E

E

K

L

V

V

I

A

A

K

N

N

I

L

R

I

F

A

W

F

A

K

D

R

G

G

H

E

R

I

P

P

D

T

Q

L

V

V

LGRI 158:161 (≠ MGRI 162:165) binding
SRT 180:182 (≠ NRH 184:186) binding
IAR 239:241 (≠ ASR 245:247) binding
HIG 288:290 (≠ HMG 294:296) binding

Q65CJ7 Hydroxyphenylpyruvate reductase; HPPR; EC 1.1.1.237 from Plectranthus scutellarioides (Coleus) (Solenostemon scutellarioides) (see paper)
41% identity, 75% coverage: 65:314/332 of query aligns to 58:299/313 of Q65CJ7

query
sites
Q65CJ7

D

D

A

A

D

E

L

L

I

I

A

D

A

A

A

L

G

-

E

P

R

K

L

L

K

E

L

I

I

V

A

S

N

S

F

F

G

S

S

V

G

G

V

L

N

D

H

K

I

V

D

D

L

L

K

I

A

K

A

C

R

E

A

E

R

K

G

G

I

V

V

R

V

V

T

T

N

N

T

T

P

P

G

D

V

V

L

L

T

T

E

D

D

D

T

V

A

A

D

D

M

L

T

A

M

I

A

G

L

L

I

I

V

L

S

A

V

V

P

L

R

R

L

I

A

C

E

E

G

C

E

D

K

K

L

Y

V

V

R

R

S

R

G

G

Q

A

W

W

K

K

G

F

W

G

S

D

P

-

G

-

G

F

M

K

L

L

G

T

H

T

R

K

I

F

G

S

G

G

K

K

K

R

L

V

G

G

I

I

V

I

G

G

M
x
L

G
|
G

R
|
R

I
|
I

G

G

Q

L

A

A

V

V

A

A

R

E

R

R

A

A

R

E

A

A

F

F

G

D

L

C

S

P

I

I

H

S

Y

Y

H

F

N
x
S

R
|
R

H
x
S

R

K

L

K

P

P

E

N

V

T

V

-

E

-

A

-

E

-

L

-

G

N

A

Y

A

T

W

Y

H

Y

G

G

D

S

L

V

D

V

A

E

M

L

L

A

R

S

E

N

I

S

D

D

I

I

L

L

T

V

I

V

H

A

T

C

P

P

L

L

N

T

E

P

E

E

S

T

R

T

D

H

L

I

I

I

D

N

A

R

R

E

R

V

I

I

G

D

L

A

L

L

G

G

P

P

Q

K

V

G

Y

V

L

L

I

I

N

N

A
x
I

S

G

R

R

G

G

G

P

I

H

V

V

D

D

E

E

E

P

A

E

M

L

V

V

D

S

A

A

L

L

E

V

A

E

G

G

R

R

L

L

A

G

G

G

A

A

G

G

L

L

D
|
D

V

V

W

F

R

E

F

R

E

E

P

P

Q

E

I

V

D

P

P

E

R

K

L

L

L

F

A

G

L

L

P

E

N

N

V

V

M

L

T

L

P

P

H

H

M

V

G

G

S

S

A

G

T

T

Y

V

E

E

G

T

R

R

H

K

A

V

T

M

G

A

E

D

K

L

V

V

I

V

A

G

N

N

I

L

LGRI 152:155 (≠ MGRI 162:165) binding
SRS 174:176 (≠ NRH 184:186) binding
I230 (≠ A245) binding
D256 (= D271) binding

3bazA Structure of hydroxyphenylpyruvate reductase from coleus blumei in complex with NADP+ (see paper)
41% identity, 75% coverage: 65:314/332 of query aligns to 56:297/311 of 3bazA

query
sites
3bazA

D

D

A

A

D

E

L

L

I

I

A

D

A

A

A

L

G

-

E

P

R

K

L

L

K

E

L

I

I

V

A

S

N

S

F

F

G

S

S
x
V

G
|
G

V

L

N

D

H

K

I

V

D

D

L

L

K

I

A

K

A

C

R

E

A

E

R

K

G

G

I

V

V

R

V

V

T

T

N

N

T

T

P

P

G

D

V

V

L
|
L

T

T

E

D

D

D

T
x
V

A

A

D

D

M

L

T

A

M

I

A

G

L

L

I

I

V

L

S

A

V

V

P

L

R

R

L

I

A

C

E

E

G

C

E

D

K

K

L

Y

V

V

R

R

S

R

G

G

Q

A

W

W

K

K

G

F

W

G

S

D

P

-

G

-

G

F

M

K

L

L

G

T

H

T

R

K

I

F

G

S

G

G

K

K

K

R

L

V

G

G

I

I

V

I

G
|
G

M
x
L

G
|
G

R
|
R

I
|
I

G

G

Q

L

A

A

V

V

A

A

R

E

R

R

A

A

R

E

A

A

F

F

G

D

L

C

S

P

I

I

H

S

Y

Y

H
x
F

N
x
S

R
|
R

H
x
S

R

K

L

K

P

P

E

N

V

T

V

-

E

-

A

-

E

-

L

-

G

N

A

Y

A

T

W

Y

H

Y

G

G

D

S

L

V

D

V

A

E

M

L

L

A

R

S

E

N

I

S

D

D

I

I

L

L

T

V

I

V

H
x
A

T
x
C

P
|
P

L

L

N

T

E

P

E

E

S
x
T

R

T

D

H

L

I

I

I

D

N

A

R

R

E

R

V

I

I

G

D

L

A

L

L

G

G

P

P

Q

K

V

G

Y

V

L

L

I

I

N

N

A
x
I

S
x
G

R
|
R

G

G

G

P

I

H

V

V

D

D

E

E

E

P

A

E

M

L

V

V

D

S

A

A

L

L

E

V

A

E

G

G

R

R

L

L

A

G

G

G

A
|
A

G

G

L

L

D
|
D

V

V

W

F

R

E

F

R

E
|
E

P

P

Q

E

I

V

D

P

P

E

R

K

L

L

L

F

A

G

L

L

P

E

N

N

V

V

M

L

T

L

P

P

H
|
H

M

V

G
|
G

S

S

A

G

T

T

Y

V

E

E

G

T

R

R

H

K

A

V

T

M

G

A

E

D

K

L

V

V

I

V

A

G

N

N

I

L

active site: L98 (= L108), R230 (= R247), A251 (= A268), D254 (= D271), E259 (= E276), H277 (= H294)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V74 (≠ S84), G75 (= G85), V102 (≠ T112), G149 (= G161), L150 (≠ M162), G151 (= G163), R152 (= R164), I153 (= I165), F171 (≠ H183), S172 (≠ N184), R173 (= R185), S174 (≠ H186), A200 (≠ H217), C201 (≠ T218), P202 (= P219), T207 (≠ S224), I228 (≠ A245), G229 (≠ S246), R230 (= R247), D254 (= D271), H277 (= H294), G279 (= G296)

5v7nA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and 2-keto-d-gluconic acid (see paper)
38% identity, 93% coverage: 8:317/332 of query aligns to 1:299/319 of 5v7nA

query
sites
5v7nA

A

S

R

R

P

P

R

R

V

I

A

L

V

V

T

P

R

G

E

K

L

I

P

N

D

P

A

R

I

V

A

L

A

E

R

R

M

L

G

P

E

E

L

M

F

F

D

E

T

T

S

V

F

-

N

-

Q

-

S

-

D

-

E

-

A

R

M

I

D

E

R

R

A

A

A

D

L

A

A

L

A

V

V

T

A

A

D

D

C

M

-

R

D

D

V

V

L

S

V

G

P

I

T

A

V

V

T
x
S

D
x
G

T

K

I

L

D

P

A

V

D

P

L

L

I

M

A

D

A

A

A

F

G

-

E

P

R

S

L

L

K

E

L

I

I

V

A

A

N

N

F
|
F

G
|
G

S
x
V

G
|
G

V

Y

N

D

H

G

I

V

D

D

L

V

K

S

A

R

A

A

R

A

A

A

R

R

G

G

I

I

V

V

T

T

N

N

T

T

P

P

G

D

V

V

L
|
L

T

T

E

E

D

E

T
x
V

A

A

D

D

M

T

T

A

M

I

A

G

L

L

I

L

V

L

S

N

V

T

P

L

R

R

R

L

L

L

A

P

E

Q

G

A

E

E

K

Q

L

W

V

L

R

R

S

Q

G

G

Q

R

W

W

-

V

-

R

K

E

G

G

W

A

S

F

P

P

G

L

G

S

M

P

L

L

G

S

H

L

R

R

I

-

G

-

G

G

K

R

K

T

L

V

G

G

I

L

V
x
F

G
|
G

M
x
L

G
|
G

R
|
R

I
|
I

G

G

Q

L

A

A

V

I

A

A

R

R

A

L

R

E

A

A

F

F

G

G

L

V

S

S

I

I

H

A

Y

Y

H
|
H

N
x
T

R
|
R

H
x
T

R

P

L

-

P

-

E

-

V

-

E

R

A

E

E

G

L

L

G

G

A

F

A

T

W

Y

H

H

G

P

D

T

L

L

D

V

A

G

M

M

L

A

R

E

E

A

I

V

D

D

I

T

L

L

T

I

I

V

H
x
I

T
x
V

P
|
P

L

G

N

T

E

A

E
x
S

S
x
T

R

L

D

K

L

A

I

V

D

N

A

A

R

D

R

V

I

L

G

S

L

A

L

L

G

G

P

P

Q

K

V

G

Y

V

L

L

I

I

N

N

A
x
V

S
x
G

R
|
R

G

G

G

S

I

T

V

V

D

D

E

E

E

A

A

A

M

L

V

V

D

T

A

A

L

L

E

Q

A

N

G

G

R

T

L

I

A

A

G

G

A

A

G

G

L

L

D
|
D

V

V

W

F

R

E

F

N

E
|
E

P

P

Q

N

I

V

D

P

P

E

R

A

L

L

A

S

L

F

P

P

N

N

V

V

V

S

M

L

T

L

P

P

H
|
H

M

V

G
x
A

S
|
S

A

A

T

S

Y

V

E

V

G

T

R
|
R

H

N

A

A

T
x
M

G

S

E

D

K

L

V

V

I

V

A

D

N

N

I

L

R

K

F

A

W

W

active site: L95 (= L108), R229 (= R247), D253 (= D271), E258 (= E276), H276 (= H294)
binding 2-keto-D-gluconic acid: S49 (≠ T61), G50 (≠ D62), F69 (= F82), G70 (= G83), V71 (≠ S84), G72 (= G85), L95 (= L108), R229 (= R247), H276 (= H294), S279 (= S297), R285 (= R303), M288 (≠ T306)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V71 (≠ S84), G72 (= G85), V99 (≠ T112), F147 (≠ V160), G148 (= G161), L149 (≠ M162), G150 (= G163), R151 (= R164), I152 (= I165), H170 (= H183), T171 (≠ N184), R172 (= R185), T173 (≠ H186), I199 (≠ H217), V200 (≠ T218), P201 (= P219), S205 (≠ E223), T206 (≠ S224), V227 (≠ A245), G228 (≠ S246), R229 (= R247), D253 (= D271), H276 (= H294), A278 (≠ G296)

5v6qB Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and malonate (see paper)
38% identity, 93% coverage: 8:317/332 of query aligns to 2:300/319 of 5v6qB

query
sites
5v6qB

A

S

R

R

P

P

R

R

V

I

A

L

V

V

T

P

R

G

E

K

L

I

P

N

D

P

A

R

I

V

A

L

A

E

R

R

M

L

G

P

E

E

L

M

F

F

D

E

T

T

S

V

F

-

N

-

Q

-

S

-

D

-

E

-

A

R

M

I

D

E

R

R

A

A

A

D

L

A

A

L

A

V

V

T

A

A

D

D

C

M

-

R

D

D

V

V

L

S

V

G

P

I

T

A

V

V

T

S

D

G

T

K

I

L

D

P

A

V

D

P

L

L

I

M

A

D

A

A

A

F

G

-

E

P

R

S

L

L

K

E

L

I

I

V

A

A

N

N

F

F

G

G

S
x
V

G
|
G

V

Y

N

D

H

G

I

V

D

D

L

V

K

S

A

R

A

A

R

A

A

A

R

R

G

G

I

I

V

V

T

T

N

N

T

T

P

P

G

D

V

V

L
|
L

T

T

E

E

D

E

T
x
V

A

A

D

D

M

T

T

A

M

I

A

G

L

L

I

L

V

L

S

N

V

T

P

L

R

R

R

L

L

L

A

P

E

Q

G

A

E

E

K

Q

L

W

V

L

R

R

S

Q

G

G

Q

R

W

W

-

V

-

R

K

E

G

G

W

A

S

F

P

P

G

L

G

S

M

P

L

L

G

S

H

L

R

R

I

-

G

-

G

G

K

R

K

T

L

V

G

G

I

L

V
x
F

G
|
G

M
x
L

G
|
G

R
|
R

I
|
I

G

G

Q

L

A

A

V

I

A

A

R

R

A

L

R

E

A

A

F

F

G

G

L

V

S

S

I

I

H

A

Y

Y

H
|
H

N
x
T

R
|
R

H
x
T

R

P

L

-

P

-

E

-

V

-

E

R

A

E

E

G

L

L

G

G

A

F

A

T

W

Y

H

H

G

P

D

T

L

L

D

V

A

G

M

M

L

A

R

E

E

A

I

V

D

D

I

T

L

L

T

I

I

V

H
x
I

T
x
V

P
|
P

L

G

N

T

E

A

E
x
S

S
x
T

R

L

D

K

L

A

I

V

D

N

A

A

R

D

R

V

I

L

G

S

L

A

L

L

G

G

P

P

Q

K

V

G

Y

V

L

L

I

I

N

N

A
x
V

S
x
G

R
|
R

G

G

G

S

I

T

V

V

D

D

E

E

E

A

A

A

M

L

V

V

D

T

A

A

L

L

E

Q

A

N

G

G

R

T

L

I

A

A

G

G

A

A

G

G

L

L

D
|
D

V

V

W

F

R

E

F

N

E
|
E

P

P

Q

N

I

V

D

P

P

E

R

A

L

L

A

S

L

F

P

P

N

N

V

V

V

S

M

L

T

L

P

P

H
|
H

M

V

G
x
A

S

S

A

A

T

S

Y

V

E

V

G

T

R

R

H

N

A

A

T

M

G

S

E

D

K

L

V

V

I

V

A

D

N

N

I

L

R

K

F

A

W

W

active site: L96 (= L108), R230 (= R247), D254 (= D271), E259 (= E276), H277 (= H294)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V72 (≠ S84), G73 (= G85), V100 (≠ T112), F148 (≠ V160), G149 (= G161), L150 (≠ M162), G151 (= G163), R152 (= R164), I153 (= I165), H171 (= H183), T172 (≠ N184), R173 (= R185), T174 (≠ H186), I200 (≠ H217), V201 (≠ T218), P202 (= P219), S206 (≠ E223), T207 (≠ S224), V228 (≠ A245), G229 (≠ S246), R230 (= R247), D254 (= D271), H277 (= H294), A279 (≠ G296)

5j23A Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with 2'-phospho-adp-ribose (see paper)
38% identity, 93% coverage: 9:317/332 of query aligns to 1:298/318 of 5j23A

query
sites
5j23A

R

R

P

P

R

R

V

I

A

L

V

V

T

P

R

G

E

K

L

I

P

N

D

P

A

R

I

V

A

L

A

E

R

R

M

L

G

P

E

E

L

M

F

F

D

E

T

T

S

V

F

-

N

-

Q

-

S

-

D

-

E

-

A

R

M

I

D

E

R

R

A

A

A

D

L

A

A

L

A

V

V

T

A

A

D

D

C

M

-

R

D

D

V

V

L

S

V

G

P

I

T

A

V

V

T

S

D

G

T

K

I

L

D

P

A

V

D

P

L

L

I

M

A

D

A

A

A

F

G

-

E

P

R

S

L

L

K

E

L

I

I

V

A

A

N

N

F

F

G

G

S
x
V

G
|
G

V

Y

N

D

H

G

I

V

D

D

L

V

K

S

A

R

A

A

R

A

A

A

R

R

G

G

I

I

V

V

T

T

N

N

T

T

P

P

G

D

V

V

L
|
L

T

T

E

E

D

E

T

V

A

A

D

D

M

T

T

A

M

I

A

G

L

L

I

L

V

L

S

N

V

T

P

L

R

R

R

L

L

L

A

P

E

Q

G

A

E

E

K

Q

L

W

V

L

R

R

S

Q

G

G

Q

R

W

W

-

V

-

R

K

E

G

G

W

A

S

F

P

P

G

L

G

S

M

P

L

L

G

S

H

L

R

R

I

-

G

-

G

G

K

R

K

T

L

V

G

G

I

L

V
x
F

G
|
G

M
x
L

G
|
G

R
|
R

I
|
I

G

G

Q

L

A

A

V

I

A

A

R

R

A

L

R

E

A

A

F

F

G

G

L

V

S

S

I

I

H

A

Y

Y

H
|
H

N
x
T

R
|
R

H
x
T

R

P

L

-

P

-

E

-

V

-

E

R

A

E

E

G

L

L

G

G

A

F

A

T

W

Y

H

H

G

P

D

T

L

L

D

V

A

G

M

M

L

A

R

E

E

A

I

V

D

D

I

T

L

L

T

I

I

V

H
x
I

T
x
V

P
|
P

L

G

N

T

E

A

E
x
S

S
x
T

R

L

D

K

L

A

I

V

D

N

A

A

R

D

R

V

I

L

G

S

L

A

L

L

G

G

P

P

Q

K

V

G

Y

V

L

L

I

I

N

N

A
x
V

S
x
G

R
|
R

G

G

G

S

I

T

V

V

D

D

E

E

E

A

A

A

M

L

V

V

D

T

A

A

L

L

E

Q

A

N

G

G

R

T

L

I

A

A

G

G

A

A

G

G

L

L

D
|
D

V

V

W

F

R

E

F

N

E
|
E

P

P

Q

N

I

V

D

P

P

E

R

A

L

L

A

S

L

F

P

P

N

N

V

V

V

S

M

L

T

L

P

P

H
|
H

M

V

G

A

S

S

A

A

T

S

Y

V

E

V

G

T

R

R

H

N

A

A

T

M

G

S

E

D

K

L

V

V

I

V

A

D

N

N

I

L

R

K

F

A

W

W

active site: L94 (= L108), R228 (= R247), D252 (= D271), E257 (= E276), H275 (= H294)
binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4r,5r)-3,4,5-trihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: V70 (≠ S84), G71 (= G85), F146 (≠ V160), G147 (= G161), L148 (≠ M162), G149 (= G163), R150 (= R164), I151 (= I165), H169 (= H183), T170 (≠ N184), R171 (= R185), T172 (≠ H186), I198 (≠ H217), V199 (≠ T218), P200 (= P219), S204 (≠ E223), T205 (≠ S224), V226 (≠ A245), G227 (≠ S246), R228 (= R247)

5v7gA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADPH and oxalate (see paper)
38% identity, 93% coverage: 9:317/332 of query aligns to 1:298/317 of 5v7gA

query
sites
5v7gA

R

R

P

P

R

R

V

I

A

L

V

V

T

P

R

G

E

K

L

I

P

N

D

P

A

R

I

V

A

L

A

E

R

R

M

L

G

P

E

E

L

M

F

F

D

E

T

T

S

V

F

-

N

-

Q

-

S

-

D

-

E

-

A

R

M

I

D

E

R

R

A

A

A

D

L

A

A

L

A

V

V

T

A

A

D

D

C

M

-

R

D

D

V

V

L

S

V

G

P

I

T

A

V

V

T

S

D

G

T

K

I

L

D

P

A

V

D

P

L

L

I

M

A

D

A

A

A

F

G

-

E

P

R

S

L

L

K

E

L

I

I

V

A

A

N

N

F

F

G
|
G

S
x
V

G
|
G

V

Y

N

D

H

G

I

V

D

D

L

V

K

S

A

R

A

A

R

A

A

A

R

R

G

G

I

I

V

V

T

T

N

N

T

T

P

P

G

D

V

V

L
|
L

T

T

E

E

D

E

T
x
V

A

A

D

D

M

T

T

A

M

I

A

G

L

L

I

L

V

L

S

N

V

T

P

L

R

R

R

L

L

L

A

P

E

Q

G

A

E

E

K

Q

L

W

V

L

R

R

S

Q

G

G

Q

R

W

W

-

V

-

R

K

E

G

G

W

A

S

F

P

P

G

L

G

S

M

P

L

L

G

S

H

L

R

R

I

-

G

-

G

G

K

R

K

T

L

V

G

G

I

L

V
x
F

G
|
G

M
x
L

G
|
G

R
|
R

I
|
I

G

G

Q

L

A

A

V

I

A

A

R

R

A

L

R

E

A

A

F

F

G

G

L

V

S

S

I

I

H

A

Y

Y

H
|
H

N
x
T

R
|
R

H
x
T

R

P

L

-

P

-

E

-

V

-

E

R

A

E

E

G

L

L

G

G

A

F

A

T

W

Y

H

H

G

P

D

T

L

L

D

V

A

G

M

M

L

A

R

E

E

A

I

V

D

D

I

T

L

L

T

I

I

V

H
x
I

T
x
V

P
|
P

L

G

N

T

E

A

E
x
S

S
x
T

R

L

D

K

L

A

I

V

D

N

A

A

R

D

R

V

I

L

G

S

L

A

L

L

G

G

P

P

Q

K

V

G

Y

V

L

L

I

I

N

N

A
x
V

S
x
G

R
|
R

G

G

G

S

I

T

V

V

D

D

E

E

E

A

A

A

M

L

V

V

D

T

A

A

L

L

E

Q

A

N

G

G

R

T

L

I

A

A

G

G

A

A

G

G

L

L

D
|
D

V

V

W

F

R

E

F

N

E
|
E

P

P

Q

N

I

V

D

P

P

E

R

A

L

L

A

S

L

F

P

P

N

N

V

V

V

S

M

L

T

L

P

P

H
|
H

M

V

G
x
A

S

S

A

A

T

S

Y

V

E

V

G

T

R

R

H

N

A

A

T

M

G

S

E

D

K

L

V

V

I

V

A

D

N

N

I

L

R

K

F

A

W

W

active site: L94 (= L108), R228 (= R247), D252 (= D271), E257 (= E276), H275 (= H294)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V70 (≠ S84), G71 (= G85), V98 (≠ T112), F146 (≠ V160), G147 (= G161), L148 (≠ M162), G149 (= G163), R150 (= R164), I151 (= I165), H169 (= H183), T170 (≠ N184), R171 (= R185), T172 (≠ H186), I198 (≠ H217), V199 (≠ T218), P200 (= P219), S204 (≠ E223), T205 (≠ S224), V226 (≠ A245), G227 (≠ S246), R228 (= R247), D252 (= D271), H275 (= H294), A277 (≠ G296)
binding oxalate ion: G69 (= G83), V70 (≠ S84), G71 (= G85), R228 (= R247), H275 (= H294)

3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
38% identity, 86% coverage: 42:327/332 of query aligns to 31:310/526 of 3dc2A

query
sites
3dc2A

D

D

R

R

A

D

A

K

L

L

A

A

V

V

A

P

D

E

C

A

D

D

V

A

L

L

V

L

P

V

T

R

V

S

T

A

D

T

T

T

I

V

D

D

A

A

D

E

L

V

I

L

A

A

G

-

E

P

R

K

L

L

K

K

L

I

I

V

A

A

N

R

F

A

G

G

S

V

G

G

V

L

N

D

H

N

I

V

D

D

L

V

K

D

A

A

R

T

A

A

R

R

G

G

I

V

V

L

V

V

T

V

N

N

T

A

P

P

G

T

V

S

L
x
N

T

I

E

H

D

S

T

A

A

A

D

E

M

H

T

A

M

L

A

A

L

L

I

L

V

L

S

A

V

A

P

S

R

R

R

Q

L

I

A

P

E

A

G

A

E

D

K

A

L

S

V

L

R

R

S

E

G

H

Q

T

W

W

K

K

G

R

W

S

S

S

P

-

G

-

M

F

L

S

G

G

H

T

R

E

I

I

G

F

G

G

K

K

K

T

L

V

G

G

I

V

V

V

G

G

M

L

G

G

R

R

I

I

G

G

Q

Q

A

L

V

V

A

A

R

Q

R

R

A

I

R

A

A

A

F

F

G

G

L

A

S

Y

I

V

H

V

Y

A

H

Y

N

D

R

P

H

Y

R

V

L

S

P

P

E

A

V

R

V

A

E

-

A

A

E

Q

L

L

G

G

A

I

A

E

W

L

H

L

G

S

D

-

L

L

D

D

A

D

M

L

L

L

R

A

E

R

I

A

D

D

I

F

L

I

T

S

I

V

H

H

T

L

P

P

L

K

N

T

E

P

E

E

S

T

R

A

D

G

L

L

I

I

D

D

A

K

R

E

R

A

I

L

G

A

L

K

L

T

G

K

P

P

Q

G

V

V

Y

I

L

I

I

V

N

N

A

A

S

A

R
|
R

G

G

I

L

V

V

D

D

E

E

E

A

A

A

M

L

V

A

D

D

A

A

L

I

E

T

A

G

G

G

R

H

L

V

A

R

G

A

A

A

G

G

L

L

D
|
D

V

V

W

F

R

A

F

T

E
|
E

P

P

Q

C

I

T

D

D

P

S

R

P

L

L

L

F

A

E

L

L

P

A

N

Q

V

V

M

V

T

T

P

P

H
|
H

M

L

G

G

S

A

A

S

T

T

Y

A

E

E

G

A

R

Q

H

D

A

R

T

A

G

G

E

T

K

D

V

V

I

A

A

E

N

S

I

V

R

R

F

L

W

A

A

L

D

A

G

G

H

E

R

F

P

V

P

P

D

D

Q

A

V

V

active site: N96 (≠ L108), R230 (= R247), D254 (= D271), E259 (= E276), H277 (= H294)

Sites not aligning to the query:

binding serine: 458, 460, 461, 462, 463, 464, 465, 484

3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
38% identity, 86% coverage: 42:327/332 of query aligns to 32:311/525 of 3ddnB

query
sites
3ddnB

D

D

R

R

A

D

A

K

L

L

A

A

V

V

A

P

D

E

C

A

D

D

V

A

L

L

V

L

P

V

T
x
R

V
x
S

T

A

D

T

T

T

I

V

D

D

A

A

D

E

L

V

I

L

A

A

G

-

E

P

R

K

L

L

K

K

L

I

I

V

A

A

N
x
R

F

A

G
|
G

S
x
V

G
|
G

V

L

N

D

H

N

I

V

D

D

L

V

K

D

A

A

R

T

A

A

R

R

G

G

I

V

V

L

V

V

T

V

N

N

T

A

P

P

G

T

V

S

L
x
N

T

I

E

H

D

S

T

A

A

A

D

E

M

H

T

A

M

L

A

A

L

L

I

L

V

L

S

A

V

A

P

S

R

R

R

Q

L

I

A

P

E

A

G

A

E

D

K

A

L

S

V

L

R

R

S

E

G

H

Q

T

W

W

K

K

G

R

W

S

S

S

P

-

G

-

M

F

L

S

G

G

H

T

R

E

I

I

G

F

G

G

K

K

K

T

L

V

G

G

I

V

V

V

G

G

M

L

G

G

R

R

I

I

G

G

Q

Q

A

L

V

V

A

A

R

Q

R

R

A

I

R

A

A

A

F

F

G

G

L

A

S

Y

I

V

H

V

Y

A

H

Y

N

D

R

P

H

Y

R

V

L

S

P

P

E

A

V

R

V

A

E

-

A

A

E

Q

L

L

G

G

A

I

A

E

W

L

H

L

G

S

D

-

L

L

D

D

A

D

M

L

L

L

R

A

E

R

I

A

D

D

I

F

L

I

T

S

I

V

H

H

T

L

P

P

L

K

N

T

E

P

E

E

S

T

R

A

D

G

L

L

I

I

D

D

A

K

R

E

R

A

I

L

G

A

L

K

L

T

G

K

P

P

Q

G

V

V

Y

I

L

I

I

V

N

N

A

A

S

A

R
|
R

G

G

I

L

V

V

D

D

E

E

E

A

A

A

M

L

V

A

D

D

A

A

L

I

E

T

A

G

G

G

R

H

L

V

A

R

G

A

A

A

G

G

L

L

D
|
D

V

V

W

F

R

A

F

T

E
|
E

P

P

Q

C

I

T

D

D

P

S

R

P

L

L

L

F

A

E

L

L

P

A

N

Q

V

V

M

V

T

T

P

P

H
|
H

M

L

G

G

S
x
A

A

S

T

T

Y

A

E

E

G

A

R
x
Q

H

D

A

R

T

A

G

G

E

T

K

D

V

V

I

A

A

E

N

S

I

V

R

R

F

L

W

A

A

L

D

A

G

G

H

E

R

F

P

V

P

P

D

D

Q

A

V

V

active site: N97 (≠ L108), R231 (= R247), D255 (= D271), E260 (= E276), H278 (= H294)
binding 2-oxo-3-(phosphonooxy)propanoic acid: R49 (≠ T59), S50 (≠ V60), R70 (≠ N81), G72 (= G83), V73 (≠ S84), G74 (= G85), N97 (≠ L108), A281 (≠ S297), Q287 (≠ R303)

4e5mA Thermostable phosphite dehydrogenase e175a/a176r in complex with NADP (see paper)
33% identity, 96% coverage: 10:327/332 of query aligns to 3:325/329 of 4e5mA

query
sites
4e5mA

P

P

R

K

V

L

A

V

V

I

T

T

R

H

E

R

L

V

P

H

D

E

A

E

I

I

A

L

A

Q

R

L

M

L

G

A

E

P

L

H

F

C

D

E

T

L

S

I

F

T

N

N

Q

Q

S

T

D

D

E

S

A

T

M

L

D

T

R

R

A

E

L

I

L

L

A

R

V

C

A

R

D

D

C

A

D

Q

V

A

L

M

V

M

P

A

T

F

V

M

T

P

D

D

T

R

I

V

D

D

A

A

D

D

L

F

I

L

A

Q

A

A

A

C

G

P

E

E

R

-

L

L

K

R

L

V

I

I

A

G

N

C

F

A

G

L

S
x
K

G
|
G

V

F

N

D

H

N

I

F

D

D

L

V

K

D

A

A

A

C

R

T

A

A

R

R

G

G

I

V

V

W

V

L

T

T

N

F

T

V

P

P

G

D

V

L

L
|
L

T

T

E

V

D

P

T
|
T

A

A

D

E

M

L

T

A

M

I

A

G

L

L

I

A

V

V

S

G

V

L

P

G

R

R

R

H

L

L

A

R

E

A

G

A

E

D

K

A

L

F

V

V

R

R

S

S

G

G

Q

Q

W

F

K

R

G

G

W

W

S

Q

P

P

G

-

G

R

M

F

L

Y

G

G

H

T

R

G

I

L

G

D

G

N

K

A

K

T

L

V

G

G

I

F

V

L

G

G

M

M

G
|
G

R
x
A

I
|
I

G

G

Q

L

A

A

V

M

A

A

R

D

R

R

A

L

R

Q

A

G

F

W

G

G

L

A

S

T

I

L

H

Q

Y

Y

H

H

N

A

R
|
R

H

K

R

A

L

L

P

D

E

T

V

Q

V

T

E

E

A

Q

E

R

L

L

G

G

A

L

A

R

W

-

H

Q

G

V

D

A

L

C

D

S

A

E

M

L

L

F

R

A

E

S

I

S

D

D

I

F

L

I

T

L

I

L

H
x
A

T
x
L

P
|
P

L

L

N

N

E

A

E

D

S
x
T

R

L

D

H

L

L

I

V

D

N

A

A

R

E

R

L

I

L

G

A

L

L

L

V

G

R

P

P

Q

G

V

A

Y

L

L

L

I

V

N

N

A
x
P

S
x
C

R
|
R

G

G

G

S

I

V

V

V

D

D

E

E

E

A

A

A

M

V

V

L

D

A

A

A

L

L

E

E

A

R

G

G

R

Q

L

L

A

G

G

G

A

Y

G

A

L

A

D
|
D

V

V

W

F

R

E

F

M

E
|
E

-

D

-

W

-

A

-

R

-

A

-

D

-

R

-

P

P

Q

Q

Q

I

I

D

D

P

P

R

A

L

L

A

A

L

H

P

P

N

N

V

T

V

L

M

F

T

T

P

P

H
|
H

M

I

G
|
G

S

S

A

A

T

V

Y

R

E

A

G

V

R

R

H

L

A

E

T

I

G

E

E

R

K

C

V

A

I

A

A

Q

N

N

I

I

R

L

F

Q

W

A

A

L

D

A

G

G

H

E

R

R

P

P

P

I

D

N

Q

A

V

V

active site: L100 (= L108), R237 (= R247), D261 (= D271), E266 (= E276), H292 (= H294)
binding nadp nicotinamide-adenine-dinucleotide phosphate: K76 (≠ S84), G77 (= G85), L100 (= L108), T104 (= T112), G154 (= G163), A155 (≠ R164), I156 (= I165), R176 (= R185), A207 (≠ H217), L208 (≠ T218), P209 (= P219), T214 (≠ S224), P235 (≠ A245), C236 (≠ S246), R237 (= R247), D261 (= D271), H292 (= H294), G294 (= G296)

4e5pA Thermostable phosphite dehydrogenase a176r variant in complex with nad (see paper)
33% identity, 96% coverage: 10:327/332 of query aligns to 3:325/332 of 4e5pA

query
sites
4e5pA

P

P

R

K

V

L

A

V

V

I

T

T

R

H

E

R

L

V

P

H

D

E

A

E

I

I

A

L

A

Q

R

L

M

L

G

A

E

P

L

H

F

C

D

E

T

L

S

I

F

T

N

N

Q

Q

S

T

D

D

E

S

A

T

M

L

D

T

R

R

A

E

L

I

L

L

A

R

V

C

A

R

D

D

C

A

D

Q

V

A

L

M

V

M

P

A

T

F

V

M

T

P

D

D

T

R

I

V

D

D

A

A

D

D

L

F

I

L

A

Q

A

A

A

C

G

P

E

E

R

-

L

L

K

R

L

V

I

I

A

G

N

C

F

A

G

L

S
x
K

G
|
G

V

F

N

D

H

N

I

F

D

D

L

V

K

D

A

A

A

C

R

T

A

A

R

R

G

G

I

V

V

W

V

L

T

T

N

F

T

V

P

P

G

D

V

L

L
|
L

T

T

E

V

D

P

T
|
T

A

A

D

E

M

L

T

A

M

I

A

G

L

L

I

A

V

V

S

G

V

L

P

G

R

R

R

H

L

L

A

R

E

A

G

A

E

D

K

A

L

F

V

V

R

R

S

S

G

G

Q

Q

W

F

K

R

G

G

W

W

S

Q

P

P

G

-

G

R

M

F

L

Y

G

G

H

T

R

G

I

L