SitesBLAST
Comparing Ga0059261_1572 FitnessBrowser__Korea:Ga0059261_1572 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
45% identity, 96% coverage: 13:471/480 of query aligns to 8:465/465 of 3pm9A
- active site: A149 (= A152), L159 (≠ T162)
- binding flavin-adenine dinucleotide: P69 (= P70), Q70 (= Q71), G71 (= G72), G72 (= G73), N73 (= N74), T74 (= T75), G75 (≠ S76), L76 (≠ M77), G79 (= G80), Q80 (≠ A81), L91 (= L94), L133 (= L136), G134 (= G137), A135 (= A138), C139 (≠ A142), T140 (= T143), G142 (= G145), G143 (= G146), S146 (= S149), T147 (= T150), A149 (= A152), G150 (= G153), E200 (= E203), G201 (= G204), I205 (≠ V208), I206 (≠ V209), E423 (= E429)
P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
33% identity, 93% coverage: 27:471/480 of query aligns to 53:496/496 of P39976
Sites not aligning to the query:
- 17 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
36% identity, 95% coverage: 14:469/480 of query aligns to 20:463/467 of 6lpnB
- binding flavin-adenine dinucleotide: W40 (= W34), P76 (= P70), G78 (= G72), G79 (= G73), N80 (= N74), T81 (= T75), G82 (≠ S76), M83 (= M77), G86 (= G80), S87 (≠ A81), L140 (= L136), A142 (= A138), C146 (≠ A142), H147 (≠ T143), G150 (= G146), N151 (≠ L147), A153 (≠ S149), T154 (= T150), G208 (= G204), I212 (≠ V208), I213 (≠ V209), E423 (= E429), N460 (= N466)
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
36% identity, 95% coverage: 14:469/480 of query aligns to 19:462/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R333), T337 (≠ S337), K348 (≠ Q348), Y379 (≠ F381), H381 (= H383), H388 (= H390), H423 (= H430)
- binding flavin-adenine dinucleotide: W39 (= W34), P75 (= P70), Q76 (= Q71), G77 (= G72), G78 (= G73), N79 (= N74), T80 (= T75), G81 (≠ S76), M82 (= M77), G85 (= G80), S86 (≠ A81), L139 (= L136), G140 (= G137), A141 (= A138), C145 (≠ A142), G149 (= G146), N150 (≠ L147), A152 (≠ S149), T153 (= T150), G157 (= G154), G207 (= G204), I212 (≠ V209), E422 (= E429), N459 (= N466)
- binding zinc ion: H381 (= H383), H388 (= H390), E422 (= E429)
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
36% identity, 95% coverage: 14:469/480 of query aligns to 19:462/466 of 6lpuA
- binding flavin-adenine dinucleotide: W39 (= W34), P75 (= P70), G77 (= G72), G78 (= G73), N79 (= N74), T80 (= T75), G81 (≠ S76), G85 (= G80), S86 (≠ A81), L139 (= L136), G140 (= G137), A141 (= A138), C145 (≠ A142), H146 (≠ T143), G148 (= G145), G149 (= G146), N150 (≠ L147), A152 (≠ S149), T153 (= T150), A155 (= A152), E206 (= E203), G207 (= G204), I211 (≠ V208), I212 (≠ V209), E422 (= E429), N459 (= N466)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R333), T337 (≠ S337), K348 (≠ Q348), Y379 (≠ F381), H381 (= H383), H388 (= H390), H423 (= H430)
- binding zinc ion: H381 (= H383), H388 (= H390), E422 (= E429)
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
36% identity, 95% coverage: 14:469/480 of query aligns to 19:462/466 of 6lpqA
- binding flavin-adenine dinucleotide: W39 (= W34), P75 (= P70), G77 (= G72), G78 (= G73), N79 (= N74), T80 (= T75), G81 (≠ S76), G85 (= G80), S86 (≠ A81), L139 (= L136), G140 (= G137), A141 (= A138), C145 (≠ A142), H146 (≠ T143), G149 (= G146), N150 (≠ L147), A152 (≠ S149), T153 (= T150), A155 (= A152), G157 (= G154), E206 (= E203), G207 (= G204), I211 (≠ V208), I212 (≠ V209), E422 (= E429), N459 (= N466)
- binding d-malate: M82 (= M77), R333 (= R333), T337 (≠ S337), K348 (≠ Q348), Y379 (≠ F381), H381 (= H383), H388 (= H390), E422 (= E429), H423 (= H430)
- binding zinc ion: H381 (= H383), H388 (= H390), E422 (= E429)
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
36% identity, 95% coverage: 14:469/480 of query aligns to 19:462/466 of 6lppA
- binding (2R)-2-hydroxypentanedioic acid: R333 (= R333), T337 (≠ S337), K348 (≠ Q348), Y379 (≠ F381), H381 (= H383), H388 (= H390), N390 (≠ H392), H423 (= H430)
- binding flavin-adenine dinucleotide: W39 (= W34), P75 (= P70), G77 (= G72), G78 (= G73), N79 (= N74), T80 (= T75), G81 (≠ S76), M82 (= M77), G85 (= G80), S86 (≠ A81), L139 (= L136), G140 (= G137), A141 (= A138), C145 (≠ A142), G149 (= G146), N150 (≠ L147), A152 (≠ S149), T153 (= T150), A155 (= A152), G157 (= G154), G207 (= G204), I212 (≠ V209), E422 (= E429), H423 (= H430)
- binding zinc ion: H381 (= H383), H388 (= H390), E422 (= E429)
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
36% identity, 95% coverage: 14:469/480 of query aligns to 72:515/521 of Q8N465
- S109 (≠ T51) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ V69) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (= G73) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (= I91) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (≠ L97) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (≠ V116) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (= P133) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (≠ S149) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (= A175) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G177) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (≠ N322) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R333) binding ; binding ; binding ; mutation to A: Loss of catalytic activity.
- T390 (≠ S337) binding ; binding ; mutation to A: Significantly reduced catalytic activity.
- V399 (≠ A346) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (≠ Q348) binding ; binding ; mutation to A: Loss of catalytic activity.
- R419 (≠ A366) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- A426 (≠ F373) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- H434 (= H383) binding ; mutation to A: Loss of catalytic activity.
- G436 (= G385) to V: slight reduction in catalytic activity
- N439 (= N388) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- H441 (= H390) binding ; mutation to A: Loss of catalytic activity.
- N443 (≠ H392) binding ; mutation to A: Significantly reduced catalytic activity.
- V444 (= V393) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- A446 (= A395) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- E475 (= E429) binding ; mutation to A: Loss of catalytic activity.
- H476 (= H430) binding ; binding ; binding ; mutation to A: Loss of catalytic activity.
- G477 (= G431) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
30% identity, 92% coverage: 32:474/480 of query aligns to 35:467/467 of 7qh2C
- binding flavin-adenine dinucleotide: V73 (≠ P70), G75 (= G72), S76 (≠ G73), G77 (≠ N74), T78 (= T75), G79 (≠ S76), L80 (≠ M77), A83 (≠ G80), C84 (≠ A81), P137 (≠ L136), G138 (= G137), E139 (≠ A138), A142 (= A142), T143 (= T143), G146 (= G146), N147 (≠ L147), S149 (= S149), T150 (= T150), A152 (= A152), G153 (= G153), E203 (= E203), G204 (= G204), I209 (≠ V209), E422 (= E429), H423 (= H430)
- binding fe (iii) ion: H377 (= H383), H384 (= H390), E422 (= E429)
P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
32% identity, 89% coverage: 47:472/480 of query aligns to 45:456/459 of P9WIT1
- K354 (≠ D360) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
27% identity, 89% coverage: 44:471/480 of query aligns to 42:454/454 of 8jdvA
- binding 2-Ketohexanoic acid: V75 (≠ M77), R317 (= R333), W321 (≠ S337), H368 (= H383), H375 (= H390), H413 (= H430)
- binding flavin-adenine dinucleotide: P68 (= P70), G70 (= G72), T71 (≠ G73), G72 (≠ N74), T73 (= T75), G74 (≠ S76), G78 (= G80), V79 (≠ A81), L90 (= L94), P132 (≠ L136), G133 (= G137), A134 (= A138), G140 (= G146), M141 (≠ L147), A143 (≠ S149), T144 (= T150), A146 (= A152), S147 (≠ G153), E200 (= E203), G201 (= G204), I206 (≠ V209), W321 (≠ S337), Y322 (≠ E338), E412 (= E429), H413 (= H430), N449 (= N466)
- binding manganese (ii) ion: H368 (= H383), H375 (= H390), E412 (= E429)
8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
26% identity, 89% coverage: 44:471/480 of query aligns to 42:455/455 of 8jdeA
- binding flavin-adenine dinucleotide: P68 (= P70), G70 (= G72), T71 (≠ G73), G72 (≠ N74), T73 (= T75), G74 (≠ S76), G78 (= G80), V79 (≠ A81), L90 (= L94), P132 (≠ L136), G133 (= G137), A134 (= A138), G140 (= G146), M141 (≠ L147), A143 (≠ S149), T144 (= T150), A146 (= A152), S147 (≠ G153), E200 (= E203), G201 (= G204), I206 (≠ V209), W322 (≠ S337), E413 (= E429), H414 (= H430), N450 (= N466)
- binding lactic acid: R318 (= R333), H369 (= H383), H376 (= H390), H414 (= H430)
- binding manganese (ii) ion: H369 (= H383), H376 (= H390), E413 (= E429)
8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
26% identity, 89% coverage: 44:471/480 of query aligns to 42:456/456 of 8jdsA
- binding flavin-adenine dinucleotide: P68 (= P70), G70 (= G72), T71 (≠ G73), G72 (≠ N74), T73 (= T75), G74 (≠ S76), G78 (= G80), V79 (≠ A81), L90 (= L94), P132 (≠ L136), G133 (= G137), A134 (= A138), G140 (= G146), M141 (≠ L147), A143 (≠ S149), T144 (= T150), A146 (= A152), S147 (≠ G153), E200 (= E203), G201 (= G204), I206 (≠ V209), W323 (≠ S337), E414 (= E429), H415 (= H430), N451 (= N466)
- binding manganese (ii) ion: H370 (= H383), H377 (= H390), E414 (= E429)
- binding pyruvic acid: R319 (= R333), H370 (= H383), H377 (= H390), H415 (= H430)
Sites not aligning to the query:
8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
26% identity, 89% coverage: 44:471/480 of query aligns to 42:455/455 of 8jdxA
- binding flavin-adenine dinucleotide: P68 (= P70), G70 (= G72), T71 (≠ G73), G72 (≠ N74), T73 (= T75), G74 (≠ S76), G78 (= G80), V79 (≠ A81), L90 (= L94), P132 (≠ L136), G133 (= G137), A134 (= A138), G140 (= G146), M141 (≠ L147), A143 (≠ S149), T144 (= T150), A146 (= A152), S147 (≠ G153), E200 (= E203), G201 (= G204), I206 (≠ V209), W322 (≠ S337), E413 (= E429), H414 (= H430), N450 (= N466)
- binding 3-methyl-2-oxobutanoic acid: R318 (= R333), H369 (= H383), H376 (= H390), H414 (= H430)
- binding manganese (ii) ion: H369 (= H383), H376 (= H390), E413 (= E429)
Sites not aligning to the query:
8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
27% identity, 89% coverage: 44:471/480 of query aligns to 42:455/455 of 8jdtA
- binding 2-ketobutyric acid: R318 (= R333), H369 (= H383), H376 (= H390), H414 (= H430)
- binding flavin-adenine dinucleotide: P68 (= P70), G70 (= G72), T71 (≠ G73), G72 (≠ N74), T73 (= T75), G74 (≠ S76), G78 (= G80), V79 (≠ A81), L90 (= L94), P132 (≠ L136), G133 (= G137), A134 (= A138), G140 (= G146), M141 (≠ L147), A143 (≠ S149), T144 (= T150), A146 (= A152), S147 (≠ G153), E200 (= E203), G201 (= G204), I206 (≠ V209), W322 (≠ S337), E413 (= E429), H414 (= H430), N450 (= N466)
- binding manganese (ii) ion: H369 (= H383), H376 (= H390), E413 (= E429)
8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
27% identity, 89% coverage: 44:471/480 of query aligns to 42:455/455 of 8jduA
- binding 2-oxopentanoic acid: R318 (= R333), W322 (≠ S337), H369 (= H383), H376 (= H390), H414 (= H430)
- binding flavin-adenine dinucleotide: P68 (= P70), G70 (= G72), T71 (≠ G73), G72 (≠ N74), T73 (= T75), G74 (≠ S76), G78 (= G80), V79 (≠ A81), L90 (= L94), P132 (≠ L136), G133 (= G137), A134 (= A138), G140 (= G146), M141 (≠ L147), A143 (≠ S149), T144 (= T150), A146 (= A152), S147 (≠ G153), E200 (= E203), G201 (= G204), I206 (≠ V209), W322 (≠ S337), E413 (= E429), N450 (= N466)
- binding manganese (ii) ion: H369 (= H383), H376 (= H390), E413 (= E429)
8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
26% identity, 89% coverage: 44:471/480 of query aligns to 42:454/454 of 8jdzA
- binding (3S)-3-methyl-2-oxopentanoic acid: R318 (= R333), W322 (≠ S337), H369 (= H383), H376 (= H390), H413 (= H430)
- binding flavin-adenine dinucleotide: P68 (= P70), G70 (= G72), T71 (≠ G73), G72 (≠ N74), T73 (= T75), G74 (≠ S76), G78 (= G80), V79 (≠ A81), L90 (= L94), P132 (≠ L136), G133 (= G137), A134 (= A138), G140 (= G146), M141 (≠ L147), A143 (≠ S149), T144 (= T150), A146 (= A152), S147 (≠ G153), E200 (= E203), G201 (= G204), I206 (≠ V209), W322 (≠ S337), E412 (= E429), H413 (= H430), N449 (= N466)
- binding manganese (ii) ion: H369 (= H383), H376 (= H390), E412 (= E429)
Sites not aligning to the query:
8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid (see paper)
26% identity, 89% coverage: 44:471/480 of query aligns to 42:454/454 of 8jdyA
- binding 2-oxo-4-methylpentanoic acid: R318 (= R333), W322 (≠ S337), S336 (≠ Q348), H369 (= H383), H376 (= H390), H413 (= H430)
- binding flavin-adenine dinucleotide: P68 (= P70), G70 (= G72), T71 (≠ G73), G72 (≠ N74), T73 (= T75), G74 (≠ S76), G78 (= G80), V79 (≠ A81), L90 (= L94), P132 (≠ L136), G133 (= G137), A134 (= A138), G140 (= G146), M141 (≠ L147), A143 (≠ S149), T144 (= T150), A146 (= A152), S147 (≠ G153), E200 (= E203), G201 (= G204), I206 (≠ V209), E412 (= E429), N449 (= N466)
- binding manganese (ii) ion: H369 (= H383), H376 (= H390), E412 (= E429)
8jdpA Crystal structure of h405a mldhd in complex with d-2-hydroxyisovaleric acid (see paper)
26% identity, 89% coverage: 44:471/480 of query aligns to 42:455/455 of 8jdpA
- binding flavin-adenine dinucleotide: P68 (= P70), G70 (= G72), T71 (≠ G73), G72 (≠ N74), T73 (= T75), G74 (≠ S76), G78 (= G80), V79 (≠ A81), L90 (= L94), P132 (≠ L136), G133 (= G137), A134 (= A138), G140 (= G146), M141 (≠ L147), A143 (≠ S149), T144 (= T150), A146 (= A152), S147 (≠ G153), E200 (= E203), G201 (= G204), I206 (≠ V209), H369 (= H383), E413 (= E429), N450 (= N466)
- binding deaminohydroxyvaline: R319 (= R333), H414 (= H430)
8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
26% identity, 89% coverage: 44:471/480 of query aligns to 42:456/456 of 8jdrA
- binding flavin-adenine dinucleotide: P68 (= P70), G70 (= G72), T71 (≠ G73), G72 (≠ N74), T73 (= T75), G74 (≠ S76), G78 (= G80), V79 (≠ A81), L90 (= L94), P132 (≠ L136), G133 (= G137), A134 (= A138), G140 (= G146), M141 (≠ L147), A143 (≠ S149), T144 (= T150), A146 (= A152), S147 (≠ G153), E200 (= E203), G201 (= G204), I206 (≠ V209), Y324 (≠ E338), H370 (= H383), E414 (= E429), N451 (= N466)
- binding (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid: R319 (= R333), W323 (≠ S337), H415 (= H430)
Query Sequence
>Ga0059261_1572 FitnessBrowser__Korea:Ga0059261_1572
MTAQQKVIDAVRARFGPRAALTDADVIAPWLNDWRGRYHGAAAAILQPDSTEAVAAMVTL
AGQHGVALVPQGGNTSMVGGATPPADGSALILSLRRLNRVRRIDAAANLAEVEAGVILAD
LHDAALAQGRRFPLTLGAKGSATVGGLVSTNAGGTQVLRFGTMRGLVLGLEAVLADGSIH
GGLAALKKDNRGYDLNQLLIGAEGTLGVVTAASLKLVAAVHARAVAWIGLASPHAGLELL
QRLEAATDTIESFEIIPARSLGAVLRHIPGTRAPLSGDHGWHVLIEAVAVSAEQEAPAQL
IERLLGPALESGLAQDATIAANEAQAEAFWKLRESISEAERASGPALQHDISVPVAAMPD
FMIDGAKAVETRFPGVSAGAFGHLGDGNVHFHVRAPAGSAADWAKTHSDIISPFVHDLVV
AAGGSISAEHGIGQMKRDELARLSSPARMHALAAIKRAFDPRNLLNPGKLVFLAPERADQ
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory