SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ga0059261_1621 FitnessBrowser__Korea:Ga0059261_1621 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q53TZ2 L-arabinose 1-dehydrogenase (NAD(P)(+)); D-galactose 1-dehydrogenase; EC 1.1.1.376; EC 1.1.1.120; EC 1.1.1.48 from Azospirillum brasilense (see paper)
53% identity, 99% coverage: 2:305/307 of query aligns to 3:307/309 of Q53TZ2

query
sites
Q53TZ2

D

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D169 (= D168) mutation to A: Loss of activity.
N173 (= N172) mutation to A: Decrease by 4 orders of magnitude in catalytic efficiency.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

7cgqA Crystal structure of azospirillum brasilense l-arabinose 1- dehydrogenase e147a mutant (NADP and l-arabinose bound form)
53% identity, 99% coverage: 3:305/307 of query aligns to 1:304/306 of 7cgqA

query
sites
7cgqA

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binding alpha-L-arabinopyranose: K88 (= K90), H116 (= H118), H150 (= H152), P167 (= P169), N170 (= N172)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G12), K11 (= K13), I12 (= I14), S34 (= S36), R35 (= R37), H36 (= H38), C64 (= C66), A65 (≠ T67), P66 (= P68), V69 (≠ G71), E87 (= E89), K88 (= K90), H116 (= H118), Q153 (= Q155), W155 (= W157), D166 (= D168), Y263 (= Y264)

2glxA Crystal structure analysis of bacterial 1,5-af reductase (see paper)
38% identity, 43% coverage: 7:137/307 of query aligns to 4:140/332 of 2glxA

query
sites
2glxA

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active site: K93 (= K90)
binding acetate ion: K93 (= K90)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G10), A8 (≠ I11), S9 (≠ G12), T10 (≠ K13), I11 (= I14), S32 (≠ A32), T33 (≠ A33), R37 (= R37), S69 (≠ C66), T70 (= T67), N72 (≠ P69), H75 (≠ R72), E92 (= E89), K93 (= K90), H121 (= H118)

Sites not aligning to the query:

active site: 179
binding acetate ion: 179
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 161, 162, 282

Q2I8V6 1,5-anhydro-D-fructose reductase; Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming); EC 1.1.1.292 from Ensifer adhaerens (Sinorhizobium morelense) (see paper)
38% identity, 43% coverage: 7:137/307 of query aligns to 5:141/333 of Q2I8V6

query
sites
Q2I8V6

G

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ASTI 9:12 (≠ IGKI 11:14) binding
S10 (≠ G12) mutation to G: Almost no effect.
A13 (= A15) mutation to G: Can use NAD as cosubstrate as well as NADP.
S33 (≠ A32) mutation to D: No activity.
ST 33:34 (≠ AA 32:33) binding
R38 (= R37) binding
TTNELH 71:76 (≠ TPPEGR 67:72) binding
EK 93:94 (= EK 89:90) binding
K94 (= K90) mutation to G: Less than 1% remaining activity.
N120 (≠ T116) binding

Sites not aligning to the query:

162:163 binding
176 D→A: Less than 1% remaining activity.
180 H→A: Less than 2% remaining activity.
206 G→I: No effect.
283 binding

1evjA Crystal structure of glucose-fructose oxidoreductase (gfor) delta1-22 s64d (see paper)
27% identity, 68% coverage: 3:212/307 of query aligns to 2:234/340 of 1evjA

query
sites
1evjA

R

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A

active site: K100 (= K90), Y188 (≠ N172)
binding nicotinamide-adenine-dinucleotide: G9 (= G10), L10 (≠ I11), G11 (= G12), K12 (= K13), Y13 (≠ I14), D35 (≠ E45), L77 (≠ T67), P78 (= P68), N79 (≠ P69), H82 (≠ R72), E99 (= E89), K100 (= K90), R128 (≠ H118), W170 (= W157), R171 (≠ I158), Y188 (≠ N172)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 267

1h6dA Oxidized precursor form of glucose-fructose oxidoreductase from zymomonas mobilis complexed with glycerol (see paper)
29% identity, 68% coverage: 3:212/307 of query aligns to 33:265/383 of 1h6dA

query
sites
1h6dA

R

R

I

F

K

G

L

Y

G

A

L

I

V

V

G
|
G

I
x
L

G
|
G

K
|
K

I
x
Y

A

A

R

L

D

N

Q

Q

H

I

L

L

P

P

A

G

I

F

A

A

A

G

S

C

V

Q

D

H

F

S

E

R

L

I

A

E

A

A

T

L

A

V

S
|
S

R
x
G

H

N

G

A

R

E

-
x
K

-

A

-

K

-

I

-

V

-

A

-

E

-

Y

-

G

V

V

D

D

G

P

V

R

E

K

G

I

H

Y

G

D

-
x
Y

S

S

I

N

V

F

E

D

L

K

I

I

A

A

A

K

R

D

P

P

E

K

I

I

D

D

A

A

L

V

S

Y

L

I

C
x
I

T
x
L

P
|
P

P
x
N

E

S

G

L

R
x
H

F

A

E

E

Q

F

A

A

M

I

A

R

A

A

I

F

A

K

A

A

G

G

K

K

H

H

V

V

M

M

L

C

E
|
E

K
|
K

P

P

P

M

A

A

A

T

T

S

V

V

S

A

E

D

A

C

R

Q

T

R

L

M

E

I

Q

D

A

A

Y

K

A

A

G

N

V

K

S

K

L

L

Y

M

A

I

T

G

W

Y

H
x
R

S

C

R

H

E

Y

A

D

G

P

E

M

V

N

D

R

A

A

R

V

D

K

W

L

L

I

A

R

A

E

R

N

R

Q

I

L

D

G

A

K

V

L

R

G

I

M

R

V

W

T

M

T

E

D

D

N

-

S

-

D

-

V

I

M

R

D

Q

Q

W

N

H

D

P

P

G
x
A

Q

Q

D

Q

W
|
W

I
x
R

L

L

-

R

-

E

A

L

A

A

G

G

F

G

G

S

V

L

F

M

D
|
D

P

I

G

G

I

I

N
x
Y

A

G

L

L

S

N

I

-

A

G

T

T

R

R

I

Y

L

L

-

L

-

G

P

E

Q

E

P

P

L

I

T

E

V

V

L

R

A

A

A

Y

T

T

M

Y

D

S

I

D

P

P

A

N

N

D

R

E

A

R

C

F

P

V

-

E

-

V

-

E

-

D

-

R

I

I

A

I

A

W

S

Q

V

M

R

R

F

F

R

R

T

S

G

G

S

A

active site: K131 (= K90), Y219 (≠ N172)
binding glycerol: K131 (= K90), R202 (≠ I158), D215 (= D168), Y219 (≠ N172)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G40 (= G10), L41 (≠ I11), G42 (= G12), K43 (= K13), Y44 (≠ I14), S66 (= S36), G67 (≠ R37), K71 (vs. gap), Y89 (vs. gap), I107 (≠ C66), L108 (≠ T67), P109 (= P68), N110 (≠ P69), H113 (≠ R72), E130 (= E89), K131 (= K90), R159 (≠ H118), A198 (≠ G154), W201 (= W157), R202 (≠ I158), Y219 (≠ N172)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 17, 18, 19, 298

1rydA Crystal structure of glucose-fructose oxidoreductase from zymomonas mobilis
29% identity, 68% coverage: 3:212/307 of query aligns to 31:263/381 of 1rydA

query
sites
1rydA

R

R

I

F

K

G

L

Y

G

A

L

I

V

V

G

G

I
x
L

G
|
G

K
|
K

I
x
Y

A

A

R

L

D

N

Q

Q

H

I

L

L

P

P

A

G

I

F

A

A

A

G

S

C

V

Q

D

H

F

S

E

R

L

I

A

E

A

A

T

L

A

V

S
|
S

R
x
G

H

N

G

A

R

E

-
x
K

-

A

-

K

-

I

-

V

-

A

-

E

-

Y

-

G

V

V

D

D

G

P

V

R

E

K

G

I

H

Y

G

D

-
x
Y

S

S

I

N

V

F

E

D

L

K

I

I

A

A

A

K

R

D

P

P

E

K

I

I

D

D

A

A

L

V

S

Y

L

I

C

I

T
x
L

P
|
P

P
x
N

E

S

G
x
L

R
x
H

F

A

E

E

Q

F

A

A

M

I

A

R

A

A

I

F

A

K

A

A

G

G

K

K

H

H

V

V

M

M

L

C

E
|
E

K
|
K

P

P

P

M

A

A

A

T

T

S

V

V

S

A

E

D

A

C

R

Q

T

R

L

M

E

I

Q

D

A

A

Y

K

A

A

G

N

V

K

S

K

L

L

Y

M

A

I

T

G

W

Y

H
x
R

S

C

R

H

E

Y

A

D

G

P

E

M

V

N

D

R

A

A

R

V

D

K

W

L

L

I

A

R

A

E

R

N

R

Q

I

L

D

G

A

K

V

L

R

G

I

M

R

V

W

T

M

T

E

D

D

N

-

S

-

D

-

V

I

M

R

D

Q

Q

W

N

H

D

P

P

G
x
A

Q

Q

D

Q

W
|
W

I
x
R

L

L

-

R

-

E

A

L

A

A

G

G

F

G

G

S

V

L

F

M

D

D

P

I

G

G

I

I

N
x
Y

A

G

L

L

S

N

I

-

A

G

T

T

R

R

I

Y

L

L

-

L

-

G

P

E

Q

E

P

P

L

I

T

E

V

V

L

R

A

A

A
x
Y

T

T

M

Y

D

S

I

D

P

P

A

N

N

D

R

E

A

R

C

F

-

V

-

E

-

V

-

E

-

D

P

R

I

I

A
x
I

A

W

S
x
Q

V

M

R

R

F

F

R

R

T

S

G

G

S

A

active site: K129 (= K90), Y217 (≠ N172)
binding alpha-D-glucopyranose: Y236 (≠ A190), I254 (≠ A203), Q256 (≠ S205)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L39 (≠ I11), G40 (= G12), K41 (= K13), Y42 (≠ I14), S64 (= S36), G65 (≠ R37), K69 (vs. gap), Y87 (vs. gap), L106 (≠ T67), P107 (= P68), N108 (≠ P69), L110 (≠ G71), H111 (≠ R72), E128 (= E89), K129 (= K90), R157 (≠ H118), A196 (≠ G154), W199 (= W157), R200 (≠ I158), Y217 (≠ N172)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 296

4koaA Crystal structure analysis of 1,5-anhydro-d-fructose reductase from sinorhizobium meliloti (see paper)
34% identity, 44% coverage: 4:137/307 of query aligns to 2:141/333 of 4koaA

query
sites
4koaA

I

I

K

R

L

W

G

G

L

L

V

I

G
|
G

I
x
A

G
x
S

K
x
T

I
|
I

A

A

R

R

D

E

Q

W

H

V

L

I

P

G

A

A

I

I

A

R

A

A

S

A

-

G

-

E

V

V

D

V

F

S

E

V

L

M

A
x
S

T

S

A

A

S

E

R
|
R

-

G

-

E

-

A

H

Y

G

A

R

A

V

E

D

N

G

G

V

I

E

A

G

K

H

A

G

V

S

T

I

S

V

V

E

D

L

D

I

L

A

V

A

G

R

D

P

P

E

D

I

V

D

D

A

A

L

V

S

Y

L

I

C

S

T
|
T

P

T

P
x
N

E

E

G

L

R
x
H

F

H

E

G

Q

Q

A

A

M

L

A

A

I

I

A

R

A

A

G

G

K

K

H

H

V

V

M

L

L

C

E

E

K
|
K

P

P

P

L

A

A

A

M

T

N

V

L

S

N

E

D

A

G

R

C

T

E

L

M

E

V

Q

L

A

K

A

A

Y

C

A

E

A

A

G

G

V

V

S

V

L

L

Y

G

A

T

T

N

W

H

H

H

S

L

R

R

E

N

A

A

G

A

E

T

V

H

D

R

A

A

A

M

R

R

D

E

W

A

L

I

A

A

R

G

R

R

I

I

active site: K94 (= K90)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), A9 (≠ I11), S10 (≠ G12), T11 (≠ K13), I12 (= I14), S33 (≠ A32), S34 (≠ A33), R38 (= R37), T71 (= T67), N73 (≠ P69), H76 (≠ R72), K94 (= K90)

Sites not aligning to the query:

active site: 180
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 160

3m2tA The crystal structure of dehydrogenase from chromobacterium violaceum
32% identity, 35% coverage: 4:111/307 of query aligns to 4:118/342 of 3m2tA

query
sites
3m2tA

I

I

K

K

L

V

G

G

L

L

V

V

G
|
G

I

I

G
|
G

K
x
A

I
x
Q

A

M

R

Q

D

E

Q

N

H

L

L

L

P

P

A

S

-

L

-

Q

-

M

-

Q

-

D

-

I

-

R

I

I

A

V

A

A

S

A

V

C

D
|
D

F
x
S

E

D

L

L

A

E

A
x
R

T

A

A

R

S

R

R

V

H

H

G

R

R

F

V

I

D

S

G

D

V

I

E

P

G

V

H

L

G

D

S

N

I

V

V

P

E

A

L

M

I

L

A

N

A

Q

R

V

P

P

E

-

I

L

D

D

A

A

L

V

S

V

L

M

C
x
A

T
x
G

P

P

E

Q

G
x
L

R

H

F

F

E

E

Q

M

A

G

M

L

A

L

A

A

I

M

A

S

A

K

G

G

K

V

H

N

V

V

M

F

L

V

E
|
E

K
|
K

P

P

A

C

A

A

T

T

V

L

S

E

E

E

A

L

R

E

T

T

L

L

E

I

Q

D

A

A

Y

R

A

R

A

S

G

D

V

V

binding nicotinamide-adenine-dinucleotide: G10 (= G10), G12 (= G12), A13 (≠ K13), Q14 (≠ I14), D36 (= D28), S37 (≠ F29), R41 (≠ A33), A73 (≠ C66), G74 (≠ T67), L78 (≠ G71), E96 (= E89), K97 (= K90)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 125, 290

7xr9A Crystal structure of dgpa with glucose (see paper)
30% identity, 36% coverage: 15:125/307 of query aligns to 16:126/344 of 7xr9A

query
sites
7xr9A

A

A

R

N

D

Q

Q

K

H

H

L

F

P

P

A

A

I

L

A

K

A

N

S

N

V

A

D

D

F

L

-

N

E

E

L

I

A

V

A

A

T

E

A

K

S

A

R

A

H

A

G

E

R

F

V

G

D

E

G

G

V

A

E

Q

G

V

H

T

G

A

S
x
D

I
x
Y

V

K

E

E

L

L

I

L

A

A

A

-

R

N

P

P

E

E

I

V

D

E

A

V

L

V

S

H

L

V

C
|
C

T
|
T

P
|
P

P

N

E

V

G

S

R
x
H

F

S

E

E

Q

I

A

T

M

I

A

A

I

F

A

E

A

A

G

G

K

K

H

H

V

V

M

Y

L

C

E
|
E

K
|
K

P
|
P

P

M

A

S

A

H

T

S

V

T

S

E

E

E

A

A

R

E

T

K

L

M

E

V

Q

E

A

A

A

W

Y

K

A

K

A

S

G

G

V

K

S

Q

L

F

Y

T

A

I

T

G

W

Y

H
x
Q

S

N

R

R

E

F

A

R

G

E

E

E

V

V

binding beta-D-glucopyranose: K91 (= K90)
binding nicotinamide-adenine-dinucleotide: D51 (≠ S49), Y52 (≠ I50), C67 (= C66), T68 (= T67), P69 (= P68), H73 (≠ R72), E90 (= E89), K91 (= K90), P92 (= P91), Q119 (≠ H118)

Sites not aligning to the query:

binding beta-D-glucopyranose: 150, 157, 160, 173, 177
binding nicotinamide-adenine-dinucleotide: 13, 14, 15, 159, 160, 166

4nheB The crystal structure of oxidoreductase (gfo/idh/moca family) from streptococcus pneumoniae tigr4 in complex with NADP
25% identity, 44% coverage: 4:137/307 of query aligns to 3:141/326 of 4nheB

query
sites
4nheB

I

L

K

K

L

L

G

G

L

V

V

I

G

G

I
x
T

G

G

K

A

I
|
I

A

S

R

H

D

-

Q

H

H

F

L

I

P

E

A

A

I

A

A

H

A

T

S

S

V

G

D

E

F

Y

E

Q

L

L

A

V

A

A

T

I

A

Y

S
|
S

R
|
R

H

K

-

L

-

E

-
x
T

-

A

-

T

-

F

-

A

G

S

R

R

V

Y

D

Q

G

N

V

I

E

Q

G

L

H

F

G

D

S

Q

I

L

V

E

E

-

L

-

I

V

A

F

R

K

P

S

E

S

I

F

D

D

A

L

L

V

S

Y

L

I

C
x
A

T
x
S

P
|
P

P
x
N

E

S

G

L

R
x
H

F

F

E

A

Q

Q

A

A

M

K

A

A

I

L

A

S

A

A

G

G

K

K

H

H

V

V

M

I

L

L

E
|
E

K
|
K

P

P

P

A

A

V

A

S

T

Q

V

P

S

Q

E

E

A

W

R

F

T

D

L

L

E

I

Q

Q

A

T

A

A

Y

E

A

K

A

N

G

N

V

C

S

F

L

I

Y

F

A

E

T

A

W

A

H
x
R

S

N

R

Y

E

H

A

E

G

K

E

A

V

F

D

T

A

T

A

I

R

K

D

N

W

F

L

L

A

A

A

D

R

K

R

Q

I

V

active site: K94 (= K90)
binding nadp nicotinamide-adenine-dinucleotide phosphate: T10 (≠ I11), I13 (= I14), S34 (= S36), R35 (= R37), T39 (vs. gap), A70 (≠ C66), S71 (≠ T67), P72 (= P68), N73 (≠ P69), H76 (≠ R72), E93 (= E89), K94 (= K90), R122 (≠ H118)

Sites not aligning to the query:

active site: 182
binding nadp nicotinamide-adenine-dinucleotide phosphate: 165, 171, 182

7d5nA Crystal structure of inositol dehydrogenase homolog complexed with nadh and myo-inositol from azotobacter vinelandii
25% identity, 58% coverage: 5:183/307 of query aligns to 23:210/379 of 7d5nA

query
sites
7d5nA

K
x
Q

L
x
I

G

G

L

Y

V

I

G

H

I

R

G

S

K

A

I

A

A

L

R

R

D

D

Q

Q

H

S

L

F

P

A

A

L

I

V

A

A

A

G

S

A

V

F

D
|
D

F
x
I

E

D

L

P

A

G

A

-

T

-

A

-

S

-

R

-

H

R

G

G

R

R

V

E

D

F

G

G

V

V

E

Q

-

L

-

G

-

V

-

D

-

P

-

Q

-

R

-

C

-

Y

-

P

G

D

H

Y

G

R

S

T

I

L

V

F

E

E

L

Q

I

E

A

A

A

R

R

R

P

P

E

D

-

G

I

I

D

Q

A

A

L

V

S

S

L

V

C

A

T
|
T

P
|
P

P
x
N

E

G

G

T

R
x
H

F

F

E

A

Q

I

A

T

M

R

A

A

I

L

A

E

A

A

G

G

K

L

H

H

V

V

M

V

L

C

E
|
E

K
|
K

P

P

P

L

A

C

A

F

T

T

V

L

S

E

E

E

A

A

R

E

T

T

L

L

E

R

Q

E

A

I

A

A

Y

L

A

A

A

N

G

N

V

R

S

V

L

V

Y

G

A

V

T

T

W

Y

H

G

S

Y

R

A

E

G

A

H

G

Q

E

L

V

I

D

E

A

Q

A

A

R

R

D

A

W

M

L

I

A

A

A

D

R

G

R

E

I

L

D

G

A

E

V

I

R

R

I

M

R

V

W

H

M

M

E

Q

-

F

-

A

-

H

-

G

-

F

-

H

-

S

-

A

-

P

-

V

D

E

I

A

R

T

Q

K

W
|
W

H
x
R

P

V

G

G

Q

P

D

S

W

Y

I

V

L

L

A

G

A

-

G

-

F

-

G

-

V

-

F

-

D

D

P

V

G

G

I

T

N
x
H

A

P

L

L

S

Y

I

L

A

S

T

E

R

V

I

M

L

L

P

P

Q

E

binding 1,4-dihydronicotinamide adenine dinucleotide: Q23 (≠ K5), I24 (≠ L6), D46 (= D28), I47 (≠ F29), T91 (= T67), P92 (= P68), N93 (≠ P69), H96 (≠ R72), E113 (= E89), K114 (= K90), W185 (= W151), R186 (≠ H152), H199 (≠ N172)

Sites not aligning to the query:

binding 1,4-dihydronicotinamide adenine dinucleotide: 17, 19, 22, 323

7d5nB Crystal structure of inositol dehydrogenase homolog complexed with nadh and myo-inositol from azotobacter vinelandii
26% identity, 58% coverage: 5:183/307 of query aligns to 23:219/389 of 7d5nB

query
sites
7d5nB

K
x
Q

L
x
I

G

G

L

Y

V

I

G

H

I

R

G

S

K

A

I

A

A

L

R

R

D

D

Q

Q

H

S

L

F

P

A

A

L

I

V

A

A

A

G

S

A

V

F

D
|
D

F
x
I

E

D

L

P

A

G

A

-

T

-

A

-

S

-

R

-

H

R

G

G

R

R

V

E

D

F

G

G

V

V

E

Q

-

L

-

G

-

V

-

D

-

P

-

Q

-

R

-

C

-

Y

-

P

G

D

H

Y

G

R

S

T

I

L

V

F

E

E

L

Q

I

E

A

A

A

R

R

R

P

P

E

D

-

G

I

I

D

Q

A

A

L

V

S

S

L

V

C

A

T
|
T

P
|
P

P
x
N

E

G

G

T

R
x
H

F

F

E

A

Q

I

A

T

M

R

A

A

I

L

A

E

A

A

G

G

K

L

H

H

V

V

M

V

L

C

E
|
E

K
|
K

P

P

P

L

A

C

A

F

T

T

V

L

S

E

E

E

A

A

R

E

T

T

L

L

E

R

Q

E

A

I

A

A

Y

L

A

A

A

N

G

N

V

R

S

V

L

V

Y

G

A

V

T

T

W

Y

H

G

S
x
Y

R

A

E

G

A

H

G

Q

E

L

V

I

D

E

A

Q

A

A

R

R

D

A

W

M

L

I

A

A

A

D

R

G

R

E

I

L

D

G

A

E

V

I

R

R

I

M

R

V

W

H

M

M

E

Q

D

F

I

A

R

H

Q

G

W

F

H

H

P

S

-

A

-

P

-

V

-

E

G

G

Q

Q

D

N

-

E

-

A

-

T

-

K

W
|
W

-
x
R

-

V

-

D

-

P

I

R

L

L

A

A

A

G

G

P

G

S

F

Y

G

V

V

L

F

G

D
|
D

P

V

G

G

I

T

N
x
H

A

P

L

L

S

Y

I

L

A

S

T

E

R

V

I

M

L

L

P

P

Q

E

binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: K114 (= K90), Y143 (≠ S119), R190 (vs. gap), D204 (= D168), H208 (≠ N172)
binding 1,4-dihydronicotinamide adenine dinucleotide: Q23 (≠ K5), I24 (≠ L6), D46 (= D28), I47 (≠ F29), T91 (= T67), P92 (= P68), N93 (≠ P69), H96 (≠ R72), E113 (= E89), K114 (= K90), W189 (= W157), R190 (vs. gap), H208 (≠ N172)

Sites not aligning to the query:

binding 1,4-dihydronicotinamide adenine dinucleotide: 17, 19, 22, 332

7d5mA Crystal structure of inositol dehydrogenase homolog complexed with NAD+ from azotobacter vinelandii
26% identity, 58% coverage: 5:183/307 of query aligns to 24:220/389 of 7d5mA

query
sites
7d5mA

K
x
Q

L
x
I

G

G

L

Y

V

I

G

H

I

R

G

S

K

A

I

A

A

L

R

R

D

D

Q

Q

H

S

L

F

P

A

A

L

I

V

A

A

A

G

S

A

V

F

D
|
D

F
x
I

E

D

L

P

A

G

A

-

T

-

A

-

S

-

R

-

H

R

G

G

R

R

V

E

D

F

G

G

V

V

E

Q

-

L

-

G

-

V

-

D

-

P

-

Q

-

R

-

C

-

Y

-

P

G

D

H

Y

G

R

S

T

I

L

V

F

E

E

L

Q

I

E

A

A

A

R

R

R

P

P

E

D

-

G

I

I

D

Q

A

A

L

V

S

S

L

V

C

A

T
|
T

P
|
P

P
x
N

E

G

G

T

R
x
H

F

F

E

A

Q

I

A

T

M

R

A

A

I

L

A

E

A

A

G

G

K

L

H

H

V

V

M

V

L

C

E
|
E

K
|
K

P

P

P

L

A

C

A

F

T

T

V

L

S

E

E

E

A

A

R

E

T

T

L

L

E

R

Q

E

A

I

A

A

Y

L

A

A

A

N

G

N

V

R

S

V

L

V

Y

G

A

V

T

T

W

Y

H

G

S

Y

R

A

E

G

A

H

G

Q

E

L

V

I

D

E

A

Q

A

A

R

R

D

A

W

M

L

I

A

A

A

D

R

G

R

E

I

L

D

G

A

E

V

I

R

R

I

M

R

V

W

H

M

M

E

Q

D

F

I

A

R

H

Q

G

W

F

H

H

P

S

-

A

-

P

-

V

-

E

G

G

Q

Q

D

N

-

E

-

A

-

T

-

K

W
|
W

-
x
R

-

V

-

D

-

P

I

R

L

L

A

A

A

G

G

P

G

S

F

Y

G

V

V

L

F

G

D

D

P

V

G

G

I

T

N
x
H

A

P

L

L

S

Y

I

L

A

S

T

E

R

V

I

M

L

L

P

P

Q

E

binding nicotinamide-adenine-dinucleotide: Q24 (≠ K5), I25 (≠ L6), D47 (= D28), I48 (≠ F29), T92 (= T67), P93 (= P68), N94 (≠ P69), H97 (≠ R72), E114 (= E89), K115 (= K90), W190 (= W157), R191 (vs. gap), H209 (≠ N172)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 18, 20, 23, 333

7xreC Crystal structure of dgpa
33% identity, 23% coverage: 54:125/307 of query aligns to 65:136/363 of 7xreC

query
sites
7xreC

I

L

A

L

A

A

R

N

P

P

E

E

I

V

D

E

A

V

L

V

S

H

L

V

C

C

T
|
T

P
|
P

P
x
N

E

V

G

S

R
x
H

F

S

E

E

Q

I

A

T

M

I

A

A

I

F

A

E

A

A

G

G

K

K

H

H

V

V

M

Y

L

C

E
|
E

K
|
K

P
|
P

P

M

A

S

A

H

T

S

V

T

S

E

E

E

A

A

R

E

T

K

L

M

E

V

Q

E

A

A

A

W

Y

K

A

K

A

S

G

G

V

K

S

Q

L

F

Y

T

A

I

T

G

W

Y

H

Q

S

N

R

R

E

F

A

R

G

E

E

E

V

V

binding nicotinamide-adenine-dinucleotide: T78 (= T67), P79 (= P68), N80 (≠ P69), H83 (≠ R72), E100 (= E89), K101 (= K90), P102 (= P91)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 13, 14, 38, 39, 170, 176, 187, 313, 314

3q2kC Crystal structure of the wlba dehydrogenase from bordetella pertussis in complex with nadh and udp-glcnaca (see paper)
31% identity, 38% coverage: 3:120/307 of query aligns to 7:130/347 of 3q2kC

query
sites
3q2kC

R

K

I

I

K

R

L

F

G

G

L

L

V

V

G

G

I

C

G
|
G

K
x
R

I
|
I

A

S

R

K

D

N

Q

-

H

H

L

I

P

G

A

A

I

I

A

A

A

Q

S

H

V

G

D

D

-

R

F

A

E

E

L

L

A

V

A

E

T

I

A

C

S
x
D

R
x
T

H

N

G

P

R

E

V

A

-

L

-

Q

-

A

-

E

-

A

D

T

G

G

V

A

E

R

G

P

H

F

G

S

S

S

I

L

V

S

E

D

L

M

I

L

A

A

A

-

R

Q

P

G

E

N

I

A

D

D

A

A

L

L

S

V

L

L

C
x
A

T
|
T

P

P

P
x
S

E

G

G

L

R
x
H

F

P

E

W

Q
|
Q

A

A

M

I

A

E

A

V

I

A

A

Q

A

A

G

G

K

R

H

H

V

V

M

V

L

S

E
|
E

K
|
K

P

P

P

M

A

A

A

T

T

R

V

W

S

E

E

D

A

G

R

K

T

R

L

M

E

V

Q

K

A

A

A

C

Y

D

A

E

A

A

G

G

V

V

S

R

L

L

Y

F

A

V

T

V

W

K

H
x
Q

S

N

R

R

active site: K100 (= K90)
binding (2S,3S,4R,5R,6R)-5-acetamido-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4-dihydroxy-oxane-2-carboxylic acid: R17 (≠ K13), K100 (= K90)
binding 1,4-dihydronicotinamide adenine dinucleotide: G16 (= G12), R17 (≠ K13), I18 (= I14), D40 (≠ S36), T41 (≠ R37), A76 (≠ C66), T77 (= T67), S79 (≠ P69), H82 (≠ R72), Q85 (= Q75), E99 (= E89), K100 (= K90), Q128 (≠ H118)

Sites not aligning to the query:

3q2kK Crystal structure of the wlba dehydrogenase from bordetella pertussis in complex with nadh and udp-glcnaca (see paper)
31% identity, 38% coverage: 3:120/307 of query aligns to 2:125/322 of 3q2kK

query
sites
3q2kK

R

K

I

I

K

R

L

F

G

G

L

L

V

V

G
|
G

I

C

G
|
G

K
x
R

I
|
I

A

S

R

K

D

N

Q

-

H

H

L

I

P

G

A

A

I

I

A

A

A

Q

S

H

V

G

D

D

-

R

-

A

-

E

-

L

F

V

E

E

L

I

A

C

A
x
D

T
|
T

A

N

S

P

R

E

H

A

G

L

R

Q

V

A

-

A

-

E

-

A

D

T

G

G

V

A

E

R

G

P

H

F

G

S

S

S

I

L

V

S

E

D

L

M

I

L

A

A

A

-

R

Q

P

G

E

N

I

A

D

D

A

A

L

L

S

V

L

L

C

A

T
|
T

P
|
P

P
x
S

E

G

G
x
L

R
x
H

F

P

E

W

Q

Q

A

A

M

I

A

E

A

V

I

A

A

Q

A

A

G

G

K

R

H

H

V

V

M

V

L

S

E
|
E

K
|
K

P

P

P

M

A

A

A

T

T

R

V

W

S

E

E

D

A

G

R

K

T

R

L

M

E

V

Q

K

A

A

A

C

Y

D

A

E

A

A

G

G

V

V

S

R

L

L

Y

F

A

V

T

V

W

K

H
x
Q

S

N

R

R

active site: K95 (= K90)
binding (2S,3S,4R,5R,6R)-5-acetamido-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4-dihydroxy-oxane-2-carboxylic acid: R12 (≠ K13), K95 (= K90)
binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G10), G11 (= G12), R12 (≠ K13), I13 (= I14), D35 (≠ A33), T36 (= T34), T72 (= T67), P73 (= P68), S74 (≠ P69), L76 (≠ G71), H77 (≠ R72), E94 (= E89), K95 (= K90), Q123 (≠ H118)

Sites not aligning to the query:

3dtyA Crystal structure of an oxidoreductase from pseudomonas syringae
28% identity, 44% coverage: 49:182/307 of query aligns to 68:196/374 of 3dtyA

query
sites
3dtyA

S

S

I

M

V

F

E

E

L

Q

I

E

A

A

A

R

R

R

P

A

E

D

-

G

I

I

D

Q

A

A

L

V

S

S

L

I

C

A

T

T

P

P

P

N

E

G

G

T

R

H

F

Y

E

S

Q

I

A

T

M

K

A

A

I

L

A

E

A

A

G

G

K

L

H

H

V

V

M

V

L

C

E

E

K
|
K

P

P

P

L

A

C

A

F

T

T

V

V

S
x
E

E

Q

A

A

R

E

T
x
N

L

L

E

R

Q

E

A

L

A

S

Y

H

A

K

A

H

G

N

V

R

S

I

L

V

Y

G

A

V

T

T

W

Y

H

G

S

Y

R

A

E

G

A

H

G

Q

E

L

V

I

D

E

A

Q

A

A

R

R

D

E

W

M

L

I

A

A

R

G

R

E

I

L

D

G

A

D

V

V

R

R

I

M

R

V

W

H

M

M

E

Q

D

F

I

A

R

H

Q

G

W

F

H

H

P

S

-

A

G

G

Q

P

D

S

W

Y

I

V

L

L

A

G

A

-

G

-

F

-

G

-

V

-

F

-

D

D

P

V

G

G

I

T

N
x
H

A

P

L

L

S

Y

I

L

A

S

T

E

R

V

I

M

L

L

P

P

active site: K110 (= K90), H186 (≠ N172)
binding magnesium ion: E117 (≠ S97), N121 (≠ T101)

3q2iA Crystal structure of the wlba dehydrognase from chromobactrium violaceum in complex with nadh and udp-glcnaca at 1.50 a resolution (see paper)
27% identity, 55% coverage: 3:170/307 of query aligns to 11:184/345 of 3q2iA

query
sites
3q2iA

R

K

I

I

K

R

L

F

G

A

L

L

V

V

G

G

I
x
C

G
|
G

K
x
R

I
|
I

A

A

R

-

D

N

Q

N

H

H

L

F

P

G

A

A

I

L

A

E

A

K

S

H

-

A

-

D

-

R

-

A

-

E

-

L

-

I

-

D

-

V

V

C

D
|
D

F
x
I

E

D

L

P

A

A

T

L

A

K

S

A

R

A

H

V

G

E

R

R

V

T

D

-

G

G

V

A

E

R

G

G

H

H

G

A

S

S

I

L

V

T

E

D

L

M

I

L

A

A

A

-

R

Q

P

T

E

D

I

A

D

D

A

I

L

V

S

I

L

L

C

T

T
|
T

P
|
P

P
x
S

E

G

G

L

R
x
H

F

P

E

T

Q

Q

A

S

M

I

A

E

A

C

I

S

A

E

A

A

G

G

K

F

H

H

V

V

M

M

L

T

E
|
E

K
|
K

P

P

P

M

A

A

A

T

T

R

V

W

S

E

E

D

A

G

R

L

T

E

L

M

E

V

Q

K

A

A

Y

D

A

K

A

A

G

K

V

K

S

H

L

L

Y

F

A

V

T

V

W

K

H
x
Q

S
x
N

R

R

E

R

A

N

G

A

E

T

V

L

D

Q

A

L

R

K

D

R

W

A

L

M

A

Q

A

E

R

K

R

R

I

F

D

G

A

R

V

I

R

-

I

-

R

-

W

Y

M

M

E

V

D

N

I

V

R

N

Q

V

-

F

W

W

H
x
T

P
x
R

G

P

Q

Q

D

E

W
x
Y

I

Y

L

D

A

A

G

G

G
x
W

F
x
R

G

G

V

T

-

W

-

E

F

F

D

D

P

G

G

G

active site: K104 (= K90)
binding (2S,3S,4R,5R,6R)-5-acetamido-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4-dihydroxy-oxane-2-carboxylic acid: K104 (= K90), N133 (≠ S119), T164 (≠ H152), R165 (≠ P153), Y169 (≠ W157)
binding 1,4-dihydronicotinamide adenine dinucleotide: C19 (≠ I11), G20 (= G12), R21 (≠ K13), I22 (= I14), D44 (= D28), I45 (≠ F29), T81 (= T67), P82 (= P68), S83 (≠ P69), H86 (≠ R72), E103 (= E89), K104 (= K90), Q132 (≠ H118), W175 (≠ G163), R176 (≠ F164)

Sites not aligning to the query:

1zh8A Crystal structure of oxidoreductase (tm0312) from thermotoga maritima at 2.50 a resolution
28% identity, 42% coverage: 3:130/307 of query aligns to 3:147/325 of 1zh8A

query
sites
1zh8A

R

K

I

I

K

R

L

L

G

G

L

I

V

V

G
|
G

I
x
C

G
|
G

K
x
I

I
x
A

A

A

R

R

D

E

Q

L

H

H

L

L

P

P

A

A

I

L

A

K

A

N

-

L

S

S

V

H

D

L

F

F

E

E

L

I

A

T

A

A

T

V

A

T

S
|
S

R
|
R

-
x
T

-

R

-

S

-
x
H

-

A

-

E

H

F

G

A

R

K

V

M

D

V

G

G

V

N

E

P

G

A

H

V

G

F

S

D

I

S

V

Y

E

E

L

E

I

L

A

L

A

E

R

S

P

G

E

L

I

V

D

D

A

A

L

V

S

D

L

L

C
x
T

T
x
L

P
|
P

P

V

E

E

G
x
L

R

N

F

L

E

P

Q

F

A

I

M

E

A

K

A

A

I

L

A

R

A

K

G

G

K

V

H

H

V

V

M

I

L

C

E
|
E

K
|
K

P

P

P

I

A

S

A

T

T

D

V

V

S

E

E

T

A

G

R

K

T

K

L

V

E

V

Q

E

A

L

A

S

Y

E

A

K

A

S

G

E

V

K

S

T

L

V

Y

Y

-

I

-

A

-

E

-
x
N

-

F

-

R

-

H

-

V

-

P

A

A

T

F

W

W

H

K

S

A

R

K

E

E

A

L

G

V

E

E

V

S

D

G

A

A

A

I

R

G

D

D

active site: K98 (= K90)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G10), C11 (≠ I11), G12 (= G12), I13 (≠ K13), A14 (≠ I14), S37 (= S36), R38 (= R37), T39 (vs. gap), H42 (vs. gap), T74 (≠ C66), L75 (≠ T67), P76 (= P68), L79 (≠ G71), E97 (= E89), K98 (= K90), N126 (vs. gap)

Sites not aligning to the query:

active site: 187
binding nadp nicotinamide-adenine-dinucleotide phosphate: 165, 170, 171, 187, 276

Query Sequence

>Ga0059261_1621 FitnessBrowser__Korea:Ga0059261_1621
VDRIKLGLVGIGKIARDQHLPAIAASVDFELAATASRHGRVDGVEGHGSIVELIAARPEI
DALSLCTPPEGRFEQAMAAIAAGKHVMLEKPPAATVSEARTLEQAAYAAGVSLYATWHSR
EAGEVDAARDWLAARRIDAVRIRWMEDIRQWHPGQDWILAAGGFGVFDPGINALSIATRI
LPQPLTVLAATMDIPANRACPIAASVRFRTGSAEVDAKFDFLQTGEQTWQIEVETDAGLL
RLTEGGGRLERPGQPSLSGPDREYPRLYRAFAELIRSGQSDVDLQPIQLVADAFLVAERR
IVDPFQF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory