SitesBLAST

2yhwA High-resolution crystal structures of n-acetylmannosamine kinase: insights about substrate specificity, activity and inhibitor modelling. (see paper)
29% identity, 37% coverage: 21:145/335 of query aligns to 7:133/309 of 2yhwA

query
sites
2yhwA

A

A

V

V

E

D

A

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G

G

T

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N

F

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L

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C

V

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A

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x
N

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N

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F

binding calcium ion: N112 (≠ T124), N115 (= N127)

Sites not aligning to the query:

binding calcium ion: 144, 161
binding zinc ion: 165, 175, 177, 182

2yi1A Crystal structure of n-acetylmannosamine kinase in complex with n- acetyl mannosamine 6-phosphate and adp. (see paper)
29% identity, 37% coverage: 21:145/335 of query aligns to 7:133/308 of 2yi1A

query
sites
2yi1A

A

A

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x
N

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x
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x
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binding adenosine-5'-diphosphate: G11 (= G25), T13 (= T27), N14 (≠ K28), R16 (≠ L30)
binding 2-acetamido-2-deoxy-6-O-phosphono-alpha-D-mannopyranose: G12 (= G26), G71 (≠ L85), G72 (≠ D86), R73 (≠ P87), S84 (≠ T98), T85 (≠ P99), L87 (vs. gap), N112 (≠ T124), D113 (= D125)
binding calcium ion: N112 (≠ T124), N115 (= N127)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 140, 189, 216, 261
binding 2-acetamido-2-deoxy-6-O-phosphono-alpha-D-mannopyranose: 139, 140, 141, 142, 162, 165, 184
binding calcium ion: 144, 161
binding zinc ion: 165, 175, 177, 182

2yhyA Structure of n-acetylmannosamine kinase in complex with n- acetylmannosamine and adp (see paper)
29% identity, 37% coverage: 21:145/335 of query aligns to 7:133/308 of 2yhyA

query
sites
2yhyA

A

A

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N

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x
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binding adenosine-5'-diphosphate: G11 (= G25), G12 (= G26), T13 (= T27), N14 (≠ K28), R16 (≠ L30)
binding calcium ion: N112 (≠ T124), N115 (= N127)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 140, 189, 216, 261
binding calcium ion: 144, 161
binding zinc ion: 165, 175, 177, 182

Q9Y223 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Homo sapiens (Human) (see 18 papers)
29% identity, 39% coverage: 15:145/335 of query aligns to 405:537/722 of Q9Y223

query
sites
Q9Y223

E

E

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x
D

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D413 (≠ E23) binding
G416 (= G26) binding
T417 (= T27) binding
N418 (≠ K28) binding
R420 (≠ L30) binding
I472 (≠ P82) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 50% of the wild-type activity; decreased N-acylmannosamine kinase activity; corresponding to less than 10% of wild-type activity
G476 (≠ D86) binding ; binding
R477 (≠ P87) binding ; binding
T489 (≠ P99) binding ; binding
N516 (≠ T124) binding ; binding
D517 (= D125) active site; binding ; binding ; mutation D->A,N: Loss of N-acylmannosamine kinase activity. Decreased affinity for N-acyl-D-mannosamine. No effect on structure.
N519 (= N127) to S: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs1554658910; mutation to S: Decreased N-acylmannosamine kinase activity.
A524 (≠ G132) to V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to less than 10% of wild-type activity; decreased N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs764698870
F528 (= F136) to C: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 70% of wild-type activity; decreased N-acylmannosamine kinase activity; dbSNP:rs986773986; mutation to C: Decreased N-acylmannosamine kinase activity.

Sites not aligning to the query:

13 C → S: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; dbSNP:rs1209266607
19 binding
23 binding
113 binding
132 H → Q: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to less than 10% of wild-type activity; impaired homohexamers formation
176 D → V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs139425890
177 R → C: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to less than 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs539332585
200 I → F: in NM; uncertain significance; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 90% of wild-type activity; decreased N-acylmannosamine kinase activity; retains 75% of wild-type activity; dbSNP:rs369328625
206 G → S: in NM; moderate phenotype with unusual involvement of quadriceps; dbSNP:rs766266918
220 binding
253 binding
259 binding
263 R → L: in SIALURIA; strong reduction of feedback inhibition by CMP-Neu5Ac; dbSNP:rs121908623
266 R → Q: in SIALURIA; abolishes feedback inhibition by CMP-Neu5Ac; dbSNP:rs121908622; R → W: in sialuria; dbSNP:rs121908621
271 binding
280 binding
281 binding
282 binding
301 binding
302 binding
303 C → V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 80% of wild-type activity; decreased N-acylmannosamine kinase activity; corresponding to 60% of wild-type activity; requires 2 nucleotide substitutions; dbSNP:rs121908633
307 binding
321 binding
331 V → A: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation
378 D → Y: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 10-30% of wild-type activity; decreased N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs199877522
545 binding
566 binding
569 binding ; binding ; binding
572 V → L: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 70-80% of wild-type activity; decreased N-acylmannosamine kinase activity; corresponding to less than 10% of wild-type activity; does not affect homohexamers formation; dbSNP:rs121908632
576 G → E: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs121908625
579 binding
581 binding
586 binding
587 I → T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs748949603; I→T: Decreased N-acylmannosamine kinase activity.
588 binding ; binding
630 A → T: in NM; decreased N-acylmannosamine kinase activity; does not affect homohexamers formation; dbSNP:rs1382191649
631 A → T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 80% of wild-type activity; decreased N-acylmannosamine kinase activity; retains 75% of wild-type activity; dbSNP:rs121908626; A → V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 70% of wild-type activity; decreased N-acylmannosamine kinase activity; does not affect homohexamers formation; dbSNP:rs62541771; mutation A->V,T: Decreased N-acylmannosamine kinase activity.
708 G → S: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; severely decreased; dbSNP:rs1554657922
712 M→T: Decreased N-acylmannosamine kinase activity.

5f7rA Rok repressor lmo0178 from listeria monocytogenes bound to inducer (see paper)
23% identity, 64% coverage: 21:233/335 of query aligns to 5:211/306 of 5f7rA

query
sites
5f7rA

A

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A

binding alpha-D-glucopyranose: K7 (≠ E23), E10 (≠ G26), G70 (≠ D86), N110 (≠ D125), N110 (≠ D125), S134 (≠ T149), V135 (= V150), G138 (= G153), L139 (≠ I154), G140 (= G155), E159 (= E172), H162 (= H175), E181 (= E199)
binding zinc ion: H162 (= H175), C172 (≠ F187), C174 (= C191), C179 (= C197)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 253, 293

5f7qE Rok repressor lmo0178 from listeria monocytogenes bound to operator (see paper)
23% identity, 59% coverage: 21:219/335 of query aligns to 86:282/396 of 5f7qE

query
sites
5f7qE

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C

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C

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G

G

D

-

C
|
C

L

L

E

E

G

M

L

Y

A

A

C

S

G

E

P

F

A

Y

M

F

K

R

A

N

R

R

W

G

G

E

A

E

A

L

A

K

E

E

T

A

L

Y

P

P

binding zinc ion: H243 (= H175), C253 (≠ F187), C255 (= C191), C260 (= C197)

Sites not aligning to the query:

binding : 5, 8, 12, 15, 32, 43, 44, 67, 68, 68, 69, 69, 70, 70, 71, 72, 73

3vglA Crystal structure of a rok family glucokinase from streptomyces griseus in complex with glucose and amppnp (see paper)
29% identity, 36% coverage: 22:143/335 of query aligns to 6:122/312 of 3vglA

query
sites
3vglA

V

V

E

D

A

I

G
|
G

G

G

T
|
T

K
|
K

F

I

L

A

C

A

G

G

I

V

A

V

D

D

R

E

T

E

G

G

S

R

V

I

L

L

A

S

Q

T

T

F

R

K

I

V

P

A

T

T

T

P

P

T

A

A

E

E

-

G

T

I

L

V

D

D

A

A

A

I

T

C

A

A

F

A

F

V

A

A

E

G

H

A

V

S

A

E

R

G

H

H

G

-

P

D

L

V

S

E

A

A

F

V

S

G

V

I

G

G

S

A

F

A

G
|
G

P

Y

L

V

S

D

L

-

D

-

P

-

I

-

A

-

P

D

D

K

Y

R

G

A

S

T

I

V

T

L

S

F

T

A

P
|
P

K

N

P

I

G

D

W

W

Q

R

D

H

V

E

D

P

L

L

L

K

G

D

Y

K

F

V

R

E

Q

Q

M

R

I

V

D

G

A

L

P

P

M

V

A

V

L

V

D

E

T
x
N

D
|
D

V

A

N

N

C

A

A

A

V

W

G

G

E

E

R

Y

L

R

F

F

G

G

S

A

G

G

R

Q

G

G

L

H

D

D

binding phosphoaminophosphonic acid-adenylate ester: G9 (= G25), T11 (= T27), K12 (= K28)
binding beta-D-glucopyranose: G65 (= G81), P78 (= P99), N103 (≠ T124), D104 (= D125)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 130, 131, 180, 214, 218, 260, 261, 264
binding beta-D-glucopyranose: 133, 134, 153, 156, 175
binding zinc ion: 156, 166, 168, 173

3vgkB Crystal structure of a rok family glucokinase from streptomyces griseus (see paper)
29% identity, 36% coverage: 22:143/335 of query aligns to 6:122/312 of 3vgkB

query
sites
3vgkB

V

V

E

D

A

I

G

G

T

T

K

K

F

I

L

A

C

A

G

G

I

V

A

V

D

D

R

E

T

E

G

G

S

R

V

I

L

L

A

S

Q

T

T

F

R

K

I

V

P

A

T

T

T

P

P

T

A

A

E

E

-

G

T

I

L

V

D

D

A

A

A

I

T

C

A

A

F

A

F

V

A

A

E

G

H

A

V

S

A

E

R

G

H

H

G

-

P

D

L

V

S

E

A

A

F

V

S

G

V

I

G

G

S

A

F

A

G

G

P

Y

L

V

S

D

L

-

D

-

P

-

I

-

A

-

P

D

D

K

Y

R

G

A

S

T

I

V

T

L

S

F

T

A

P

P

K

N

P

I

G

D

W

W

Q

R

D

H

V

E

D

P

L

L

L

K

G

D

Y

K

F

V

R

E

Q

Q

M

R

I

V

D

G

A

L

P

P

M

V

A

V

L

V

D

E

T

N

D

D

V

A

N

N

C

A

A

A

V

W

G

G

E

E

R

Y

L

R

F

F

G

G

S

A

G

G

R

Q

G

G

L

H

D

D

Sites not aligning to the query:

binding zinc ion: 156, 166, 168, 173

O35826 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Rattus norvegicus (Rat) (see paper)
28% identity, 39% coverage: 15:145/335 of query aligns to 405:537/722 of O35826

query
sites
O35826

E

E

P
x
T

L
|
L

L
x
S

L
x
A

G
x
L

A
|
A

V
|
V

E
x
D

A
x
L

G
|
G

T
|
T

K
x
N

F
x
L

L
x
R

C
x
V

G
x
A

I
|
I

A
x
V

D
x
S

R
x
M

T
x
K

G
|
G

S
x
E

V
x
I

L
x
V

A
x
K

Q
x
K

-
x
Y

T
|
T

R
x
Q

I
x
F

P
x
N

T
x
P

T
x
K

T
|
T

P
x
Y

A
x
E

E
|
E

T
x
R

L
x
I

D
x
S

A
x
L

A
x
I

T
x
L

A
x
Q

F
x
M

F
x
C

A
x
V

E
|
E

H
x
A

V
x
A

A
|
A

R
x
E

H
x
A

G
x
V

P
x
K

L
|
L

S
x
N

A
x
C

F
x
R

S
x
I

V
x
L

G
|
G

S

-

F
x
V

G
|
G

P
x
I

L
x
S

S
x
T

L
x
G

D
x
G

P
x
R

I
x
V

A
x
N

P
|
P

D
x
Q

Y
x
E

G
|
G

S
x
V

I
x
V

T
x
L

S
x
H

T
x
S

P
x
T

K
|
K

-
x
L

-
x
I

P
x
Q

G
x
E

W
|
W

Q
x
N

D
x
S

V
|
V

D
|
D

L
|
L

L
x
R

G
x
T

Y
x
P

F
x
L

R
x
S

Q
x
D

M
x
T

I
x
L

D
x
H

A
x
L

P
|
P

M
x
V

A
x
W

L
x
V

D
|
D

T
x
N

D
|
D

V
x
G

N
|
N

C
|
C

A
|
A

V
x
M

G
x
A

E
|
E

R
|
R

L
x
K

F
|
F

G
|
G

S
x
Q

G
|
G

R
x
K

G
|
G

L
x
Q

D
x
E

T
x
N

F
|
F

D413 (≠ E23) mutation D->K,N: No effect on UDP-N-acetylglucosamine 2-epimerase activity. Does not affect feedback inhibition by CMP-Neu5Ac. Loss of N-acylmannosamine kinase activity. Does not interfere with oligomerization.
R420 (≠ L30) mutation to M: No effect on UDP-N-acetylglucosamine 2-epimerase activity. Does not affect feedback inhibition by CMP-Neu5Ac. Loss of N-acylmannosamine kinase activity. Does not interfere with oligomerization.

Sites not aligning to the query:

1 UDP-N-acetylglucosamine 2-epimerase
49 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Does not interfere with enzyme oligomerization.
110 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Partial reduction of the dimerization process.
132 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Partial reduction of the dimerization process.
155 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Strong reduction of the dimerization process.
157 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Strong reduction of the dimerization process.
406:722 N-acetylmannosamine kinase

7p9lAAA Ubiquitin-like protein SMT3,N-acetyl-D-glucosamine kinase
27% identity, 47% coverage: 23:179/335 of query aligns to 7:162/303 of 7p9lAAA

query
sites
7p9lAAA

E

D

A

I

G

G

T

T

K

K

F

I

L

E

C

F

G

G

I

A

A

F

D

D

R

A

T

D

G

L

S

V

V

R

L

V

A

A

Q

R

T

E

R

R

I

V

-

A

-

T

P

P

T

T

T

E

T

S

P

Y

A

A

E

A

T

F

L

L

D

D

A

A

A

I

T

V

A

T

F

L

F

V

A

N

E

N

H

A

V

D

A

A

R

E

H

F

G

G

P

V

L

K

S

G

A

T

F

V

S

G

V

I

G

G

S

I

F
x
P

G
|
G

P

I

L

A

S

D

L

V

D

E

P

T

I

-

A

-

P

-

D

-

Y

-

G

G

S

K

I

L

T

L

S

T

T
x
S

P

N

K

I

P

P

G

A

W

A

Q

M

D

G

V

H

D

T

L

L

L

Q

G

R

Y

D

F

L

R

E

Q

E

M

R

I

L

D

Q

A

R

P

P

M

V

A

K

L

I

D

E

T
x
N

D
|
D

V

A

N

N

C

C

A

F

A

A

V

L

G

S

E

E

R

A

L

W

F

D

G

E

S

D

G

L

R

R

G

G

L

E

D

P

T

S

F

V

C

L

Y

G

V

L

T

I

V

L

G
|
G

T
|
T

G
|
G

I
x
V

G
|
G

V

G

G

G

L

L

L

I

V

F

G

N

G

G

A

K

P

V

H

H

G

S

G

G

A

R

N

A

H

N

P

I

-

A

-

G

E
|
E

A

I

G

G

H
|
H

I

T

R

R

L

L

P

P

binding 2-acetamido-2-deoxy-6-O-phosphono-beta-D-glucopyranose: P66 (≠ F80), G67 (= G81), S79 (≠ T98), N105 (≠ T124), D106 (= D125), G132 (= G151), T133 (= T152), G134 (= G153), V135 (≠ I154), G136 (= G155), E155 (= E172), H158 (= H175)
binding zinc ion: H158 (= H175)

Sites not aligning to the query:

7p7wBBB Ubiquitin-like protein SMT3,N-acetyl-D-glucosamine kinase
27% identity, 47% coverage: 23:179/335 of query aligns to 10:165/306 of 7p7wBBB

query
sites
7p7wBBB

E

D

A

I

G

G

T

T

K

K

F

I

L

E

C

F

G

G

I

A

A

F

D

D

R

A

T

D

G

L

S

V

V

R

L

V

A

A

Q

R

T

E

R

R

I

V

-

A

-

T

P

P

T

T

T

E

T

S

P

Y

A

A

E

A

T

F

L

L

D

D

A

A

A

I

T

V

A

T

F

L

F

V

A

N

E

N

H

A

V

D

A

A

R

E

H

F

G

G

P

V

L

K

S

G

A

T

F

V

S

G

V

I

G

G

S

I

F

P

G

G

P

I

L

A

S

D

L

V

D

E

P

T

I

-

A

-

P

-

D

-

Y

-

G

G

S

K

I

L

T

L

S

T

T

S

P

N

K

I

P

P

G

A

W

A

Q

M

D

G

V

H

D

T

L

L

L

Q

G

R

Y

D

F

L

R

E

Q

E

M

R

I

L

D

Q

A

R

P

P

M

V

A

K

L

I

D

E

T

N

D

D

V

A

N

N

C

C

A

F

A

A

V

L

G

S

E

E

R

A

L

W

F

D

G

E

S

D

G

L

R

R

G

G

L

E

D

P

T

S

F

V

C

L

Y

G

V

L

T

I

V

L

G
|
G

T
|
T

G

G

I

V

G

G

V

G

G

G

L

L

L

I

V

F

G

N

G

G

A

K

P

V

H

H

G

S

G

G

A

R

N

A

H

N

P

I

-

A

-

G

E

E

A

I

G

G

H
|
H

I

T

R

R

L

L

P

P

binding adenosine-5'-diphosphate: G135 (= G151), T136 (= T152)
binding zinc ion: H161 (= H175)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 196, 200, 258, 259, 262
binding zinc ion: 182, 184, 189

7p9pAAA Ubiquitin-like protein SMT3,N-acetyl-D-glucosamine kinase
27% identity, 47% coverage: 23:179/335 of query aligns to 8:163/304 of 7p9pAAA

query
sites
7p9pAAA

E

D

A

I

G

G

G
|
G

T
|
T

K
|
K

F

I

L

E

C

F

G

G

I

A

A

F

D

D

R

A

T

D

G

L

S

V

V

R

L

V

A

A

Q

R

T

E

R

R

I

V

-

A

-

T

P

P

T

T

T

E

T

S

P

Y

A

A

E

A

T

F

L

L

D

D

A

A

A

I

T

V

A

T

F

L

F

V

A

N

E

N

H

A

V

D

A

A

R

E

H

F

G

G

P

V

L

K

S

G

A

T

F

V

S

G

V

I

G

G

S

I

F

P

G

G

P

I

L

A

S

D

L

V

D

E

P

T

I

-

A

-

P

-

D

-

Y

-

G

G

S

K

I

L

T

L

S

T

T

S

P

N

K

I

P

P

G

A

W

A

Q

M

D

G

V

H

D

T

L

L

L

Q

G

R

Y

D

F

L

R

E

Q

E

M

R

I

L

D

Q

A

R

P

P

M

V

A

K

L

I

D

E

T

N

D

D

V

A

N

N

C

C

A

F

A

A

V

L

G

S

E

E

R

A

L

W

F

D

G

E

S

D

G

L

R

R

G

G

L

E

D

P

T

S

F

V

C

L

Y

G

V

L

T

I

V

L

G
|
G

T
|
T

G

G

I

V

G

G

V

G

G

G

L

L

L

I

V

F

G

N

G

G

A

K

P

V

H

H

G

S

G

G

A

R

N

A

H

N

P

I

-

A

-

G

E

E

A

I

G

G

H
|
H

I

T

R

R

L

L

P

P

binding phosphoaminophosphonic acid-adenylate ester: G11 (= G26), T12 (= T27), K13 (= K28), G133 (= G151), T134 (= T152)
binding zinc ion: H159 (= H175)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 194, 198, 211, 256, 257, 260
binding zinc ion: 180, 182, 187, 213, 217

Query Sequence

>Ga0059261_1776 Ga0059261_1776 Transcriptional regulator/sugar kinase
MPLDSFGQAGLMGDEPLLLGAVEAGGTKFLCGIADRTGSVLAQTRIPTTTPAETLDAATA
FFAEHVARHGPLSAFSVGSFGPLSLDPIAPDYGSITSTPKPGWQDVDLLGYFRQMIDAPM
ALDTDVNCAAVGERLFGSGRGLDTFCYVTVGTGIGVGLLVGGAPHGGANHPEAGHIRLPR
APGDHDFAGICPFHGDCLEGLACGPAMKARWGAAAETLPGDHPAWDIEADYLAGLCATLT
YIVRPDRIILGGGVMESHLMHARVRRTLVAKLAGYDASMRSLDMDEYVVPPTAGPSAGLT
GAFALAYRIVTRQWPMHWAATGSLIAPSITEFANA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory