SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ga0059261_1823 FitnessBrowser__Korea:Ga0059261_1823 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q8C165 N-fatty-acyl-amino acid synthase/hydrolase PM20D1; Peptidase M20 domain-containing protein 1; PM20D1; EC 3.5.1.114; EC 3.5.1.14 from Mus musculus (Mouse) (see paper)
28% identity, 78% coverage: 95:453/463 of query aligns to 116:482/503 of Q8C165

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H125 (= H104) mutation to A: Loss of N-fatty-acyl-amino acid synthase/hydrolase activity.
D127 (= D106) mutation to A: Loss of N-fatty-acyl-amino acid synthase/hydrolase activity.
H465 (= H436) mutation to A: Loss of N-fatty-acyl-amino acid synthase/hydrolase activity.

7uoiA Crystallographic structure of dape from enterococcus faecium (see paper)
37% identity, 28% coverage: 30:158/463 of query aligns to 9:129/383 of 7uoiA

query
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binding zinc ion: H75 (= H104), D108 (= D136)

Sites not aligning to the query:

binding zinc ion: 168

1lfwA Crystal structure of pepv (see paper)
45% identity, 19% coverage: 84:172/463 of query aligns to 66:155/468 of 1lfwA

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active site: H87 (= H104), D119 (= D136), E153 (= E170), E154 (= E171)
binding 3-[(1-amino-2-carboxy-ethyl)-hydroxy-phosphinoyl]-2-methyl-propionic acid: H87 (= H104), D119 (= D136), E153 (= E170), E154 (= E171)
binding zinc ion: H87 (= H104), D119 (= D136), D119 (= D136), E154 (= E171)

Sites not aligning to the query:

P45494 Beta-Ala-Xaa dipeptidase; Beta-Ala-His dipeptidase; Peptidase V; EC 3.4.13.- from Lactobacillus delbrueckii subsp. lactis (see paper)
45% identity, 19% coverage: 84:172/463 of query aligns to 66:155/470 of P45494

query
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P45494

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H87 (= H104) binding Zn(2+)
D119 (= D136) binding Zn(2+); binding Zn(2+)
E154 (= E171) binding Zn(2+)

Sites not aligning to the query:

177 binding Zn(2+)
439 binding Zn(2+)

4o23A Crystal structure of mono-zinc form of succinyl diaminopimelate desuccinylase from neisseria meningitidis mc58 (see paper)
32% identity, 31% coverage: 28:171/463 of query aligns to 2:135/376 of 4o23A

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binding zinc ion: H67 (= H104), D100 (= D136)

Sites not aligning to the query:

binding zinc ion: 163

4pqaA Crystal structure of succinyl-diaminopimelate desuccinylase from neisseria meningitidis mc58 in complex with the inhibitor captopril (see paper)
32% identity, 31% coverage: 28:171/463 of query aligns to 2:135/375 of 4pqaA

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binding l-captopril: D100 (= D136), E134 (= E170), E135 (= E171)
binding zinc ion: H67 (= H104), D100 (= D136), D100 (= D136), E135 (= E171)

Sites not aligning to the query:

7t1qA Crystal structure of the succinyl-diaminopimelate desuccinylase (dape) from acinetobacter baumannii in complex with succinic acid
37% identity, 25% coverage: 58:171/463 of query aligns to 29:135/377 of 7t1qA

query
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7t1qA

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binding zinc ion: H67 (= H104), D100 (= D136), D100 (= D136), E135 (= E171)

Sites not aligning to the query:

binding zinc ion: 163, 349

4op4B Crystal structure of the catalytic domain of dape protein from v.Cholerea in the zn bound form (see paper)
32% identity, 31% coverage: 28:171/463 of query aligns to 2:135/265 of 4op4B

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4op4B

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G

F

I

V

I

F

-

A

G

G

H
|
H

M

T

D

D

V

V

A

P

A

A

D

G

P

P

-

L

K

S

D

Q

W

W

E

H

R

T

D

P

P

P

F

F

T

E

P

P

V

T

I

V

E

I

N

D

G

G

Y

F

I

L

F

H

G

G

R

R

G

G

S

A

T

A

D
|
D

N

M

K

K

F

G

S

S

V

L

S

A

A

C

M

M

V

I

A

V

A

A

L

V

I

E

Q

R

L

F

K

I

K

A

D

E

G

H

F

P

K

D

P

H

R

Q

R

G

D

S

I

I

I

G

F

F

M

L

G

I

S

T

G

S

D

D

E

E

E
|
E

binding zinc ion: H67 (= H104), D100 (= D136), D100 (= D136), E135 (= E171)

Sites not aligning to the query:

binding zinc ion: 163, 238

2pokA Crystal structure of a m20 family metallo peptidase from streptococcus pneumoniae
26% identity, 69% coverage: 32:352/463 of query aligns to 24:353/458 of 2pokA

query
sites
2pokA

L

F

E

E

L

V

L

L

K

R

R

T

A

L

I

I

A

S

F

K

R

K

T

S

V

V

E

F

G

A

P

Q

G

Q

N

V

Q

G

T

L

P

K

D

E

Y

V

A

A

N

N

Y

Y

L

L

R

G

D

E

A

I

L

F

I

K

A

R

G

V

G

G

F

-

A

-

E

A

D

E

V

V

T

E

V

I

E

D

R

E

L

S

G

Y

D

T

T

A

A

P

M

F

L

V

V

M

A

A

R

H

Y

F

R

K

G

-

T

S

A

S

K

R

P

P

G

-

T

D

A

A

K
|
K

P

T

I

L

G

I

I

F

I

Y

G

N

H
|
H

M

Y

D

D

V

T

V

V

A

P

A

A

D

D

-

G

P

D

K

Q

D

V

W

W

E

T

R

E

D

D

P

P

F

F

T

T

P

L

V

S

I

V

E

R

N

N

G

G

Y

F

I

M

F

Y

G

G

R

R

G

G

S

V

T

D

D
|
D

N

D

K

K

F

G

S

H

V

I

S

T

A

A

M

R

V

L

A

S

A

A

L

L

I

R

Q

K

L

Y

K

M

K

Q

D

H

G

H

F

D

K

D

P

L

R

P

R

V

D

N

I

I

I

S

F

F

M

I

G

M

S

E

G

G

D

A

E
|
E

E

E

-

S

-

A

-

S

T

T

E

D

M

L

-

D

K

K

T

Y

T

L

A

E

A

K

L

H

A

A

E

D

K

K

Y

L

T

R

G

G

L

A

E
x
D

L

L

L

V

-

W

-
x
E

-
x
Q

-

G

-

T

-

K

-

N

-

A

-

L

-

E

N

Q

I

L

D

E

A

I

G

S

G

G

R

N

Y

-

S

-

P

-

E

K

G

G

K

I

A

V

E

T

Y

F

Y

-

G

-

L

-

Q

-

A

D

A

A

E

K

K

V

T

K

Y

S

A

A

D

D

F

V

I

D

L

I

T

H

V

S

T

S

D

Y

P

G

G

G

G

V

H

V

S

E

S

S

A

A

P

P

R

W

P

Y

-

L

V

L

N

Q

A

A

I

L

V

Q

T

S

L

L

A

R

R

A

A

A

V

D

E

G

R

R

I

I

-

L

-

V

-

E

G

G

N

L

Y

Y

K

E

F

E

A

V

P
x
Q

E

E

V

P

N
|
N

E

E

I

R

T
x
E

K

M

A

A

S

L

L

L

A

E

V

T

A

Y

A

G

E

Q

T

R

A

-

K

N

P

P

E

E

D

E

A

V

A

S

A

R

I

I

R

Y

-

G

-

L

-

E

-

L

-

P

-

L

-

Q

-

E

-

R

-

M

A

A

F

F

L

L

A

K

N

R

P

F

Q

F

D

F

D

D

G

P

A

A

L

L

K

N

V

I

L

E

R

G

A

I

N

Q

Y

S

G

G

L

Y

V

Q

G

G

Q

Q

I

-

G

G

T

V

T

K

C

T

V

I

P

-

T

-

M

-

V

-

N

-

A

-

G

-

H

-

A

-

Q

-

N

-

A

-

L

L

P

P

Q

A

R

E

A

A

T

S

A

A

N

K

I

L

N

E

C

V

R

R

I

L

F

V

P

P

G

G

-

L

-

E

-

P

-

H

N

D

S

V

I

L

E

E

S

K

I

I

Q

R

A

K

R

Q

L

L

binding beta-D-glucopyranose: K84 (= K97), D180 (≠ E188), Q257 (≠ P258), N260 (= N261), E263 (≠ T264)
binding manganese (ii) ion: H91 (= H104), D124 (= D136), E158 (= E170), E185 (vs. gap), Q186 (vs. gap)

Sites not aligning to the query:

binding beta-D-glucopyranose: 373, 374, 375, 379, 456

4mmoA The crystal structure of a m20 family metallo-carboxypeptidase sso-cp2 from sulfolobus solfataricus
26% identity, 47% coverage: 32:248/463 of query aligns to 9:215/437 of 4mmoA

query
sites
4mmoA

L

I

E

E

L

F

L

L

K

K

R

K

A

P

I

-

A

-

F

-

R

-

T

S

V

I

E

S

G

A

P

T

G

G

N

E

Q

G

T

I

P

D

D

E

Y

T

A

A

N

N

Y

Y

L

L

R

K

D

E

A

T

L

V

I

-

A

-

G

E

G

K

F

L

A

L

A

G

E

V

D

K

V

A

T

N

V

L

E

E

R

K

L

T

G

K

D

G

T

H

A

P

M

V

L

V

V

Y

A

A

R

E

Y

I

R

N

G

V

T

N

A

A

K

K

P

-

G

-

T

-

A

-

K

K

P

T

I

L

G

L

I

I

I

Y

G

N

H
|
H

M

Y

D

D

V

V

V

Q

A

P

A

V

D

D

P

P

-

I

K

S

D

E

W

W

E

K

R

R

D

A

P

P

F

F

T

S

P

A

V

T

I

I

E

E

N

N

G

D

Y

R

I

I

F

Y

G

A

R

R

G

G

S

A

T

S

D
|
D

N

N

K

K

F

G

S

T

V

L

S

M

A

A

M

R

V

L

A

F

A

A

L

I

I

K

Q

H

L

L

K

-

K

L

D

D

G

K

F

N

K

E

P

L

R

N

R

V

D

N

I

V

I

K

F

L

M

L

G

Y

S

E

G

G

D

E

E

E

T

I

E

G

M

S

K

V

T

N

T

L

A

E

A

D

L

Y

A

I

E

E

K

K

Y

N

T

T

G

N

L

K

E

L

L

K

L

A

L

D

N

S

I

V

D

I

A

M

G
x
E

G

G

G

A

R

G

Y

L

S

D

P

P

E

K

G

G

K

R

A

P

E

Q

Y

I

Y

V

G

-

L

L

Q

G

A

V

A

K

E

G

K

L

T

L

Y

Y

A

V

D

E

F

L

I

V

L

L

T

D

V

Y

T

G

D

T

P

K

G

D

G

L

H

H

S

S

-

N

A

A

P

P

R

L

P

V

V

R

N

N

A

P

I

C

V

I

T

D

L

L

A

A

R

K

A

I

V

I

binding zinc ion: H71 (= H104), D104 (= D136), E163 (≠ G196)

Q96KP4 Cytosolic non-specific dipeptidase; CNDP dipeptidase 2; Glutamate carboxypeptidase-like protein 1; Peptidase A; Threonyl dipeptidase; EC 3.4.13.18 from Homo sapiens (Human)
39% identity, 16% coverage: 97:172/463 of query aligns to 92:168/475 of Q96KP4

query
sites
Q96KP4

K

K

P

T

I

V

G

C

I

I

I

Y

G

G

H
|
H

M

L

D

D

V

V

V

Q

A

P

A

A

D

A

P

L

K

E

D

D

-

G

W

W

E

D

R

S

D

E

P

P

F

F

T

T

P

L

V

V

I

E

E

R

N

D

G

G

Y

K

I

L

F
x
Y

G

G

R

R

G

G

S

S

T

T

D
|
D

N

D

K

K

F

G

S

P

V

V

S

A

A

G

M

W

V

I

A

N

A

A

L

L

I

E

Q

A

L

Y

K

Q

K

K

D

T

G

G

F

Q

K

E

P

I

R

P

R

V

D

N

I

V

I

R

F

F

M

C

G

L

S

E

G

G

D

M

E

E

E
|
E

T

S

H99 (= H104) binding Mn(2+)
Y126 (≠ F130) to H: in dbSNP:rs2278161
D132 (= D136) binding Mn(2+); binding Mn(2+)
E167 (= E171) binding Mn(2+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
195 binding Mn(2+)
445 binding Mn(2+)

5vo3A Crystal structure of dape in complex with the products (succinic acid and diaminopimelic acid) (see paper)
37% identity, 18% coverage: 91:175/463 of query aligns to 57:143/380 of 5vo3A

query
sites
5vo3A

A

A

K

K

P

H

G

G

T

T

A

S

K

E

P

P

-

V

I

I

G

A

I

F

I

A

G

G

H
|
H

M

T

D

D

V

V

-

P

A

T

A

G

D

D

P

E

K

N

D

Q

W

W

E

S

R

S

D

P

P

P

F

F

T

S

P

A

V

E

I

I

E

I

N

D

G

G

Y

M

I

L

F

Y

G

G

R

R

G

G

S

A

T

A

D
|
D

N

M

K

K

F

G

S

S

V

L

S

A

A

A

M

M

V

I

A

V

A

A

L

A

I

E

Q

E

L

Y

K

V

K

K

D

A

G

N

F

P

K

N

P

H

R

K

R

G

D

T

I

I

I

A

F

L

M

L

G

I

S

T

G

S

D

D

E
|
E

T
x
A

E

T

M

A

K

K

binding 2,6-diaminopimelic acid: E138 (= E170), E139 (= E171), A140 (≠ T172)
binding succinic acid: D104 (= D136), E138 (= E170), E139 (= E171)
binding zinc ion: H71 (= H104), D104 (= D136), D104 (= D136), E139 (= E171)

Sites not aligning to the query:

binding 2,6-diaminopimelic acid: 185, 262, 294, 328, 329
binding succinic acid: 167, 182, 328, 329, 353
binding zinc ion: 167, 353

P44514 Succinyl-diaminopimelate desuccinylase; SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase; EC 3.5.1.18 from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) (see 3 papers)
37% identity, 18% coverage: 91:175/463 of query aligns to 53:139/377 of P44514

query
sites
P44514

A

A

K

K

P

H

G

G

T

T

A

S

K

E

P

P

-

V

I

I

G

A

I

F

I

A

G

G

H
|
H

M

T

D

D

V

V

-

P

A

T

A

G

D

D

P

E

K

N

D

Q

W

W

E

S

R

S

D

P

P

P

F

F

T

S

P

A

V

E

I

I

E

I

N

D

G

G

Y

M

I

L

F

Y

G

G

R

R

G

G

S

A

T

A

D
|
D

N

M

K

K

F

G

S

S

V

L

S

A

A

A

M

M

V

I

A

V

A

A

L

A

I

E

Q

E

L

Y

K

V

K

K

D

A

G

N

F

P

K

N

P

H

R

K

R

G

D

T

I

I

I

A

F

L

M

L

G

I

S

T

G

S

D

D

E
|
E

T

A

E

T

M

A

K

K

H67 (= H104) binding Zn(2+); mutation to A: Reduction of affinity for L,L-SDAP and of catalytic efficiency.
D100 (= D136) binding Zn(2+); binding Zn(2+)
E134 (= E170) active site, Proton acceptor; mutation to A: Absence of desuccinylase activity.; mutation to D: Reduction of the catalytic efficiency.
E135 (= E171) binding Zn(2+)

Sites not aligning to the query:

163 binding Zn(2+)
349 binding Zn(2+); H→A: Absence of desuccinylase activity and of zinc ion.

4h2kA Crystal structure of the catalytic domain of succinyl-diaminopimelate desuccinylase from haemophilus influenzae (see paper)
37% identity, 18% coverage: 91:175/463 of query aligns to 55:141/258 of 4h2kA

query
sites
4h2kA

A

A

K

K

P

H

G

G

T

T

A

S

K

E

P

P

-

V

I

I

G

A

I

F

I

A

G

G

H
|
H

M

T

D

D

V

V

-

P

A

T

A

G

D

D

P

E

K

N

D

Q

W

W

E

S

R

S

D

P

P

P

F

F

T

S

P

A

V

E

I

I

E

I

N

D

G

G

Y

M

I

L

F

Y

G

G

R

R

G

G

S

A

T

A

D
|
D

N

M

K

K

F

G

S

S

V

L

S

A

A

A

M

M

V

I

A

V

A

A

L

A

I

E

Q

E

L

Y

K

V

K

K

D

A

G

N

F

P

K

N

P

H

R

K

R

G

D

T

I

I

I

A

F

L

M

L

G

I

S

T

G

S

D

D

E

E

E
|
E

T

A

E

T

M

A

K

K

binding zinc ion: H69 (= H104), D102 (= D136), D102 (= D136), E137 (= E171)

Sites not aligning to the query:

binding zinc ion: 165, 232

2zogA Crystal structure of mouse carnosinase cn2 complexed with zn and bestatin (see paper)
39% identity, 16% coverage: 97:172/463 of query aligns to 96:172/478 of 2zogA

query
sites
2zogA

K

K

P

T

I

V

G

C

I

I

I

Y

G

G

H
|
H

M

L

D

D

V

V

V

Q

A

P

A

A

D

A

P

L

K

E

D

D

-

G

W

W

E

D

R

S

D

E

P

P

F

F

T

T

P

L

V

V

I

E

E

R

N

E

G

G

Y

K

I

L

F

Y

G

G

R

R

G

G

S

S

T

T

D
|
D

N

D

K

K

F

G

S

P

V

V

S

A

A

G

M

W

V

M

A

N

A

A

L

L

I

E

Q

A

L

Y

K

Q

K

K

D

T

G

G

F

Q

K

E

P

I

R

P

R

V

D

N

I

L

I

R

F

F

M

C

G

L

S

E

G

G

D

M

E
|
E

T

S

binding 2-(3-amino-2-hydroxy-4-phenyl-butyrylamino)-4-methyl-pentanoic acid: H103 (= H104), D136 (= D136), E170 (= E170), E171 (= E171)
binding zinc ion: H103 (= H104), D136 (= D136), D136 (= D136), E171 (= E171)

Sites not aligning to the query:

2zofA Crystal structure of mouse carnosinase cn2 complexed with mn and bestatin (see paper)
39% identity, 16% coverage: 97:172/463 of query aligns to 96:172/478 of 2zofA

query
sites
2zofA

K

K

P

T

I

V

G

C

I

I

I

Y

G

G

H
|
H

M

L

D

D

V

V

V

Q

A

P

A

A

D

A

P

L

K

E

D

D

-

G

W

W

E

D

R

S

D

E

P

P

F

F

T

T

P

L

V

V

I

E

E

R

N

E

G

G

Y

K

I

L

F

Y

G

G

R

R

G

G

S

S

T

T

D
|
D

N

D

K

K

F

G

S

P

V

V

S

A

A

G

M

W

V

M

A

N

A

A

L

L

I

E

Q

A

L

Y

K

Q

K

K

D

T

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G

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binding 2-(3-amino-2-hydroxy-4-phenyl-butyrylamino)-4-methyl-pentanoic acid: D136 (= D136), E170 (= E170), E171 (= E171)
binding manganese (ii) ion: H103 (= H104), D136 (= D136), D136 (= D136), E171 (= E171)

Sites not aligning to the query:

Q9D1A2 Cytosolic non-specific dipeptidase; CNDP dipeptidase 2; Glutamate carboxypeptidase-like protein 1; Threonyl dipeptidase; EC 3.4.13.18 from Mus musculus (Mouse) (see 2 papers)
39% identity, 16% coverage: 97:172/463 of query aligns to 92:168/475 of Q9D1A2

query
sites
Q9D1A2

K

K

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D

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H99 (= H104) binding Mn(2+)
D132 (= D136) binding Mn(2+); binding Mn(2+)
E166 (= E170) mutation to A: Loss of threonyl dipeptidase activity.
E167 (= E171) binding Mn(2+)

Sites not aligning to the query:

195 binding Mn(2+)
228 H→A: Loss of activity.
445 binding Mn(2+)

3pfoA Crystal structure of a putative acetylornithine deacetylase (rpa2325) from rhodopseudomonas palustris cga009 at 1.90 a resolution
33% identity, 17% coverage: 94:172/463 of query aligns to 94:173/426 of 3pfoA

query
sites
3pfoA

G

G

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A

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binding zinc ion: H104 (= H104), D137 (= D136), D137 (= D136), E172 (= E171)

Sites not aligning to the query:

binding zinc ion: 195, 395

7lgpB Dape enzyme from shigella flexneri
34% identity, 24% coverage: 61:171/463 of query aligns to 29:136/377 of 7lgpB

query
sites
7lgpB

A

A

L

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binding zinc ion: H68 (= H104), D101 (= D136)

Sites not aligning to the query:

binding zinc ion: 164

8uw6B Acetylornithine deacetylase from escherichia coli, di-zinc form.
29% identity, 32% coverage: 103:251/463 of query aligns to 77:214/381 of 8uw6B

query
sites
8uw6B

G

G

H
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H

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D

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binding zinc ion: H78 (= H104), D110 (= D136), D110 (= D136), E143 (= E171), E167 (= E207)

Sites not aligning to the query:

binding zinc ion: 353, 376, 381

Query Sequence

>Ga0059261_1823 FitnessBrowser__Korea:Ga0059261_1823
MRSMIAALAMTVAAAGVAHAQKAAPEADAQALELLKRAIAFRTVEGPGNQTPDYANYLRD
ALIAGGFAAEDVTVERLGDTAMLVARYRGTAKPGTAKPIGIIGHMDVVAADPKDWERDPF
TPVIENGYIFGRGSTDNKFSVSAMVAALIQLKKDGFKPRRDIIFMGSGDEETEMKTTAAL
AEKYTGLELLLNIDAGGGRYSPEGKAEYYGLQAAEKTYADFILTVTDPGGHSSAPRPVNA
IVTLARAVERIGNYKFAPEVNEITKASLAVAAETAKPEDAAAIRAFLANPQDDGALKVLR
ANYGLVGQIGTTCVPTMVNAGHAQNALPQRATANINCRIFPGNSIESIQARLKDVAAEPG
LTIVKDDSGGKPGPASPLRPDVMKAVTDGIHARFPTVKVIPSMSAGATDSMHFRGRGVPS
YGIAPIFMKQSDQFAHGLNERTPLSELAPAVTYFRRVVAELSK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory