SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ga0059261_2227 FitnessBrowser__Korea:Ga0059261_2227 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P05041 Aminodeoxychorismate synthase component 1; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 1; EC 2.6.1.85 from Escherichia coli (strain K12) (see 4 papers)
42% identity, 44% coverage: 113:373/587 of query aligns to 185:443/453 of P05041

query
sites
P05041

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K

E258 (= E186) mutation to A: The reaction is extremely slow.; mutation to D: The reaction is extremely slow.
K274 (= K202) mutation to A: Absence of covalent intermediate. Addition of ammonia allows the formation of the covalent intermediate and shows that ammonia can replace the function of K-274. Reduced catalytic efficiency.; mutation to R: Absence of covalent intermediate.; mutation to R: Reduced catalytic efficiency.
G275 (= G203) mutation to S: Catalytically inactive for both the glutamine-dependent and ammonia-dependent reactions and fails to interact with PabA.
R311 (= R239) mutation to K: Catalytically active in the NH3-dependent, but inactive for the glutamine-dependent reactions and fails to complex with PabA.
R316 (= R244) mutation to H: Catalytically inactive for both the glutamine-dependent and ammonia-dependent reactions and fails to interact with PabA.
S322 (= S250) mutation to T: Complete loss of aminodeoxychorismate synthase activity.
H339 (≠ T267) mutation to W: Catalytically inactive for both the glutamine-dependent and ammonia-dependent reactions and fails to interact with PabA.

Sites not aligning to the query:

36 binding

P28820 Aminodeoxychorismate synthase component 1; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 1; EC 2.6.1.85 from Bacillus subtilis (strain 168) (see paper)
40% identity, 44% coverage: 121:379/587 of query aligns to 202:458/470 of P28820

query
sites
P28820

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A283 (≠ K202) mutation to I: Complete loss of aminodeoxychorismate synthase activity.; mutation to K: Absence of covalent intermediate.; mutation to V: Complete loss of aminodeoxychorismate synthase activity.

1k0eA The crystal structure of aminodeoxychorismate synthase from formate grown crystals (see paper)
41% identity, 44% coverage: 113:373/587 of query aligns to 183:427/437 of 1k0eA

query
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1k0eA

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active site: E256 (= E186), K272 (= K202), E286 (= E230), H323 (≠ T267), S350 (= S294), W374 (≠ Y318), R394 (= R338), G410 (= G356), E423 (= E369), K427 (= K373)
binding tryptophan: P238 (≠ G168), F239 (≠ Y169), S240 (≠ G170)

Sites not aligning to the query:

binding tryptophan: 32, 33, 34, 41, 44

7pi1DDD Aminodeoxychorismate synthase component 1
40% identity, 44% coverage: 121:379/587 of query aligns to 195:451/459 of 7pi1DDD

query
sites
7pi1DDD

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binding magnesium ion: G428 (= G356), E438 (= E366)
binding tryptophan: P242 (≠ G168), Y243 (= Y169), M244 (≠ G170), Q406 (≠ A332), N408 (= N334)

Sites not aligning to the query:

binding tryptophan: 33, 34, 35, 39, 41

Q94GF1 Anthranilate synthase alpha subunit 1, chloroplastic; OsASA1; EC 4.1.3.27 from Oryza sativa subsp. japonica (Rice) (see paper)
36% identity, 48% coverage: 113:393/587 of query aligns to 283:576/577 of Q94GF1

query
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Q94GF1

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S

S

V

V

R

K

V

V

P

E

E

K

L

L

F

M

K

N

V

V

E

E

T

R

Y

Y

P

S

T

H

L

V

H

M

T

H

M

I

V

S

S

S

T

T

V

V

S

T

A

G

R

E

L

L

A

R

D

D

G

D

T

L

G

T

I

C

A

W

D

D

V

A

L

L

A

R

A

A

T

A

F

L

P

P

C

V

G

G

S

T

I

V

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

V

L

I

I

D

D

A

Q

V

M

E

E

P

G

D

K

A

M

R

R

G

G

P

P

Y

Y

T

S

G

G

S

G

I

F

G

G

R

G

I

V

D

S

P

F

N

R

G

G

D

D

A

M

A

D

F

I

N

A

V

L

A

A

I

L

R

R

T

T

L

I

H

V

L

F

P

P

-

T

-

G

-

S

-

R

-

F

-

D

-

T

-

M

-

Y

-

S

-

Y

-

T

-

D

-

K

-

N

-

A

R

R

V

Q

S

E

E

W

T

V

A

A

S

H

I

L

G

Q

L

A

G

G

S

A

G

G

V

I

V

V

A

A

D

D

S

S

R

K

A

P

G

D

E

D

E

E

W

H

D

Q

E

E

C

C

L

L

A

N

K

K

G

A

A

A

F

G

L

L

T

A

R

R

G

A

Q

-

R

-

Q

-

V

I

D

D

L

L

I

A

E

E

T

S

M

T

A

F

F

V

D

D

D323 (= D153) mutation to N: Insensitive to feedback inhibition by tryptophan.

8hx8A Crystal structure of 4-amino-4-deoxychorismate synthase from streptomyces venezuelae co-crystallized with chorismate (see paper)
43% identity, 44% coverage: 118:373/587 of query aligns to 405:662/673 of 8hx8A

query
sites
8hx8A

R

R

A

H

A

D

Y

K

D

D

A

A

A

Y

F

L

A

K

R

R

V

I

H

D

D

E

W

C

-

L

-

K

-

E

I

I

E

R

A

N

G

G

D

E

I

S

Y

Y

Q

E

A

I

N

C

L

L

S

T

F

N

R

M

A

V

S

T

V

A

R

P

M

T

L

E

G

A

D

T

P

A

L

L

A

P

A

L

Y

Y

A

S

A

A

I

L

R

R

A

A

R

I

A

S

A

P

A

V

G
x
P

Y
|
Y

G
|
G

G

A

I

L

V

L

W

E

T

F

G

P

D

E

D

L

W

S

L

V

L

L

S

S

F

A

S

S

P

P

E

E

L

R

F

F

F

L

A

T

L

I

-

G

R

A

D

D

G

G

E

G

L

V

I

E

A

S

R

K

P

P

M

I

K

K

G

G

T

T

T

R

P

P

R

R

G

G

A

G

T

T

P

A

E

E

D

D

E

E

A

R

A

L

R

R

A

A

T

D

L

L

A

A

T

G

D

R

A

E

K

K

Q

D

R

R

A

A

E
|
E

N

N

L

L

M

M

I

I

V

V

D

D

L

L

M

V

R

R

N

N

D

D

L

L

T

N

R

S

I

V

A

C

A

A

P

I

G

G

S

S

V

V

R

H

V

V

P

P

E

R

L

L

F

F

K

E

V

V

E

E

T

T

Y

Y

P

A

T

P

L

V

H

H

T

Q

M

L

V

V

S

S

T

T

V

I

S

R

A

G

R

R

L

L

A

R

D

P

G

G

T

T

G

S

I

T

A

A

D

A

V

C

L

V

A

R

A

A

T

A

F

F

P

P

C

G

G

G

S

S

I

M

T

T

G

G

A

A

P

P

K

K

I

K

R

R

A

T

M

M

E

E

V

I

I

I

D

D

A

R

V

L

E

E

P

E

D

G

A

P

R

R

G

G

P

V

Y

Y

T

S

G

G

S

A

I

L

G
|
G

R

W

I

F

D

A

P

L

N

S

G

G

D

A

A

A

A

D

F

L

N

S

V

I

A

V

I

I

R

R

T

T

L

I

H

V

L

L

P

-

R

-

V

A

S

D

E

G

T

Q

A

A

S

E

I

F

G

G

L

V

G

G

S

G

G

A

V

I

V

V

A

S

D

L

S

S

R

D

A

Q

G

E

E

E

E
|
E

W

F

D

T

E
|
E

C

T

L

V

A

V

K

K

binding magnesium ion: E521 (= E230), E655 (= E366), E658 (= E369)
binding tryptophan: P458 (≠ G168), Y459 (= Y169), G460 (= G170), G614 (= G323)

Sites not aligning to the query:

binding magnesium ion: 175, 177
binding tryptophan: 231, 232, 233, 241, 243

8hx9A Crystal structure of 4-amino-4-deoxychorismate synthase from streptomyces venezuelae with chorismate (see paper)
43% identity, 44% coverage: 118:373/587 of query aligns to 366:623/632 of 8hx9A

query
sites
8hx9A

R

R

A

H

A

D

Y

K

D

D

A

A

A

Y

F

L

A

K

R

R

V

I

H

D

D

E

W

C

-

L

-

K

-

E

I

I

E

R

A

N

G

G

D

E

I

S

Y

Y

Q

E

A

I

N

C

L

L

S

T

F

N

R

M

A

V

S

T

V

A

R

P

M

T

L

E

G

A

D

T

P

A

L

L

A

P

A

L

Y

Y

A

S

A

A

I

L

R

R

A

A

R

I

A

S

A

P

A

V

G
x
P

Y
|
Y

G
|
G

G

A

I

L

V

L

W

E

T

F

G

P

D

E

D

L

W

S

L

V

L

L

S

S

F

A

S

S

P

P

E

E

L

R

F

F

F

L

A

T

L

I

-

G

R

A

D

D

G

G

E

G

L

V

I

E

A

S

R

K

P

P

M
x
I

K
|
K

G
|
G

T
|
T

T

R

P

P

R

R

G

G

A

G

T

T

P

A

E

E

D

D

E

E

A

R

A

L

R

R

A

A

T

D

L

L

A

A

T

G

D

R

A

E

K

K

Q

D

R

R

A

A

E

E

N

N

L

L

M

M

I

I

V

V

D

D

L

L

M

V

R

R

N

N

D

D

L

L

T

N

R

S

I

V

A

C

A

A

P

I

G

G

S

S

V

V

R

H

V

V

P

P

E

R

L

L

F

F

K

E

V

V

E

E

T

T

Y

Y

P

A

T

P

L

V

H

H

T

Q

M

L

V

V

S

S

T

T

V

I

S

R

A

G

R

R

L

L

A

R

D

P

G

G

T

T

G

S

I

T

A

A

D

A

V

C

L

V

A

R

A

A

T

A

F

F

P

P

C

G

G

G

S

S

I
x
M

T

T

G

G

A

A

P

P

K

K

I

K

R

R

A

T

M

M

E

E

V

I

I

I

D

D

A

R

V

L

E

E

P

E

D

G

A

P

R

R

G

G

P

V

Y
|
Y

T

S

G

G

S

A

I
x
L

G
|
G

R

W

I

F

D

A

P

L

N

S

G

G

D

A

A

A

A

D

F

L

N

S

V

I

A

V

I

I

R
|
R

T

T

L

I

H

V

L

L

P

-

R

-

V

A

S

D

E

G

T

Q

A

A

S

E

I

F

G

G

L
x
V

G
|
G

S
x
G

G
x
A

V

I

V

V

A

S

D

L

S

S

R

D

A

Q

G

E

E

E

W

F

D

T

E
|
E

C

T

L

V

A

V

K
|
K

binding (3R,4R)-3-[(1-carboxyethenyl)oxy]-4-hydroxycyclohexa-1,5-diene-1-carboxylic acid: I453 (≠ M201), K454 (= K202), G455 (= G203), T456 (= T204), M547 (≠ I295), Y570 (= Y318), R590 (= R338), V603 (≠ L353), G604 (= G354), G605 (≠ S355), A606 (≠ G356), E619 (= E369), K623 (= K373)
binding tryptophan: P419 (≠ G168), Y420 (= Y169), G421 (= G170), L574 (≠ I322), G575 (= G323)

Sites not aligning to the query:

binding magnesium ion: 134, 135
binding tryptophan: 189, 190, 191, 199, 201

P32068 Anthranilate synthase alpha subunit 1, chloroplastic; Anthranilate synthase component 1-1; Anthranilate synthase component I-1; Protein A-METHYL TRYPTOPHAN RESISTANT 1; Protein JASMONATE-INDUCED DEFECTIVE LATERAL ROOT 1; Protein TRYPTOPHAN BIOSYNTHESIS 5; Protein WEAK ETHYLENE INSENSITIVE 2; EC 4.1.3.27 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
36% identity, 46% coverage: 121:389/587 of query aligns to 309:590/595 of P32068

query
sites
P32068

Y

Y

D

K

A

E

A

A

F

V

A

V

R

K

V

A

H

K

D

E

W

H

I

I

E

L

A

A

G

G

D

D

I

I

Y

F

Q

Q

A

I

N

V

L

L

S

S

F

Q

R

R

A

F

S

E

V

R

R

R

M

T

L

F

G

A

D
|
D

P

P

L

F

A

E

A

V

Y

Y

A

R

A

A

I

L

R

R

A

V

R

V

A

N

A

P

A

S

G

P

Y

Y

G

M

G

G

I

Y

V

L

W

Q

T

A

G

R

D

G

D

C

W

I

L

L

L

V

S

A

F

S

S

S

P

P

E

E

L

I

F

L

F

T

A

K

L

V

R

K

D

Q

G

N

E

K

L

I

I

V

A

N

R

R

P

P

M

L

K

A

G

G

T

T

T

S

P

K

R

R

G

G

A

K

T

N

P

E

E

V

E

E

D

D

E

K

A

R

A

L

R

E

A

K

T

E

L

L

A

L

T

E

D

N

A

E

K

K

Q

Q

R

C

A

A

E

E

N

H

L

I

M

M

I

L

V

V

D

D

L

L

M

G

R

R

N

N

D

D

L

V

T

G

R

K

I

V

A

T

A

K

P

Y

G

G

S

S

V

V

R

K

V

V

P

E

E

K

L

L

F

M

K

N

V

I

E

E

T

R

Y

Y

P

S

T

H

L

V

H

M

T

H

M

I

V

S

S

S

T

T

V

V

S

T

A

G

R

E

L

L

A

Q

D

D

G

G

T

L

G

T

I

C

A

W

D

D

V

V

L

L

A

R

A

A

T

A

F

L

P

P

C

V

G

G

S

T

I

V

T

S

G

G

A

A

P

P

K

K

I

V

R

K

A

A

M

M

E

E

V

L

I

I

D

D

A

E

V

L

E

E

P

P

D

T

A

R

R

R

G

G

P

P

Y

Y

T

S

G

G

S

G

I

F

G

G

R

G

I

V

D

S

P

F

N

T

G

G

D

D

A

M

A

D

F

I

N

A

V

L

A

S

I

L

R

R

T

T

L

I

H

V

L

F

P

P

R

T

V

A

S

C

E

Q

-

Y

-

N

-

T

-

M

-

Y

-

S

-

Y

-

K

-

D

-

A

-

N

-

K

-

R

-

E

-

W

T

V

A

A

S

Y

I

L

G

Q

L

A

G

G

S

A

G

G

V

V

A

A

D

D

S

S

R

D

A

P

G

Q

E

D

E

E

W

H

D

C

E

E

C

C

L

Q

A

N

K

K

G

A

A

A

F

G

L

L

T

A

R

R

G

A

Q

-

R

-

Q

-

V

I

D

D

L

L

I

A

E

E

T

S

D341 (= D153) mutation to N: In trp5-1; insensitive to feedback inhibition by tryptophan and resistance to the herbicide 6-methylanthranilate.

A0QX93 Anthranilate synthase component 1; AS; ASI; EC 4.1.3.27 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
41% identity, 41% coverage: 132:373/587 of query aligns to 262:507/524 of A0QX93

query
sites
A0QX93

I

I

E

E

A

A

G

G

D

E

I

A

Y

F

Q

Q

A

V

N

V

L

P

S

S

F

Q

R

R

A

F

S

E

V

M

R

D

M

T

L

A

G

A

D

D

P

P

L

L

A

D

A

V

Y

Y

A

R

A

I

I

L

R

R

A

V

R

T

A

N

A

P

A

S

G

P

Y

Y

G

M

G

Y

I

L

V

L

W

N

T

I

G

P

D

D

-

A

D

D

W

G

L

G

L

L

S

D

F

F

S

S

-

I

-

V

-

G

-

S

P

P

E

E

L

A

F

L

F

V

A

T

L

V

R

K

D

D

G

G

E

R

L

A

I

T

A

T

R

H

P

P

M

I

K

A

G

G

T

T

T

R

P

W

R

R

G

G

A

A

T

T

P

E

E

E

D

D

E

V

A

L

R

E

A
x
K

T

E

L

L

A

L

T

A

D

D

A

E

K

K

Q

E

R

R

A

A

E

E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

M

G

R

R

N

N

D

D

L

L

T

G

R

R

I

V

A

C

A

R

P

P

G

G

S

T

V

V

R

R

V

V

P

D

E

D

L

Y

F

S

K

H

V

I

E

E

T

R

Y

Y

P

S

T

H

L

V

H

M

T

H

M

L

V

V

S

S

T

T

V

V

S

T

A

G

R

E

L

L

A

A

D

E

G

D

T

K

G

T

I

A

A

L

D

D

V

A

L

V

A

T

A

A

T

C

F

F

P

P

C

A

G

G

S

T

I

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

V

L

I

I

D

E

A

E

V

V

E

E

P

K

D

T

A

R

R

R

G

G

P

L

Y

Y

T

G

G

G

S

V

I

V

G

G

R

Y

I

L

D

D

P

F

N

A

G

G

D

N

A

A

A

D

F

F

N

A

V

I

A

A

I

I

R

R

T

T

L

A

H

L

L

M

P

R

R

-

V

-

S

N

E

G

T

T

A

A

S

Y

I

V

G

Q

L

A

G

G

S

G

G

G

V

V

A

A

D

D

S

S

R

N

A

G

G

P

E

Y

E

E

W

Y

D

T

E

E

C

A

L

A

A

N

K

K

K355 (≠ A219) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

1k0gA The crystal structure of aminodeoxychorismate synthase from phosphate grown crystals (see paper)
36% identity, 44% coverage: 113:373/587 of query aligns to 185:410/420 of 1k0gA

query
sites
1k0gA

Q

Q

P

S

G

N

I

M

M

T

R

R

A

E

A

Q

Y

Y

D

G

A

E

A

K

F

F

A

R

R

Q

V

V

H

Q

D

E

W

Y

I

L

E

H

A

S

G

G

D

D

I

C

Y

Y

Q

Q

A

V

N

N

L

L

S

A

F

Q

R

R

A

F

S

H

V

A

R

T

M

Y

L

S

G

G

D

D

P

E

L

W

A

Q

A

A

Y

F

A

L

A

Q

I

L

R

N

A

Q

R

A

A

N

A

R

A

A

G
x
P

Y
x
F

G
x
S

G

A

I

F

V

L

W

R

T

L

G

E

D

Q

D

G

W

A

L

I

L

L

S

S

F

L

S

S

P

P

E
|
E

L

R

F

F

F

I

A

L

L

C

R

D

D

N

G

S

E

E

L

I

I

Q

A

T

R

R

P

P

M

I

K
|
K

G

D

T

-

P

-

R

-

G

-

A

-

T

-

P

-

E

-

D

-

E

-

A

-

R

-

A

-

T

-

L

-

A

-

T

-

D

-

A

-

K

-

Q

-

R

R

A

A

E
|
E

N

N

L

L

M

M

I

I

V

V

D

D

L

L

M

M

R

R

N

N

D

D

L

I

T

G

R

R

I

V

A

A

A

V

P

A

G

G

S

S

V

V

R

K

V

V

P

P

E

E

L

L

F

F

K

-

V

-

E

-

T

-

Y

-

P

-

T

-

L

-

H

-

T

V

M

L

V

V

S

S

T

T

V

I

S

T

A

A

R

Q

L

L

A

P

D

E

G

Q

T

L

G

H

I

A

A

S

D

D

V

L

L

L

A

R

A

A

T

A

F

F

P

P

C

G

G

G

S
|
S

I

I

T

T

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

V

I

I

I

D
|
D

A

E

V

L

E

E

P

P

D

Q

A
x
R

R

R

G

N

P

A

Y
x
W

T

C

G

G

S

S

I

I

G

G

R

Y

I

L

D

S

P

F

N

C

G

G

D

N

A

M

A

D

F

T

N

S

V

I

A

T

I

I

R
|
R

T

T

L

-

H

-

L

L

P

T

R

A

V

I

S

N

E

G

T

Q

A

I

S

F

I

C

G

S

L

A

G

G

S

G

G
|
G

V

I

V

V

A

A

D

D

S

S

R

Q

A

E

G

E

E

A

E

E

W

Y

D

Q

E
|
E

C

T

L

F

A

D

K
|
K

active site: E258 (= E186), K274 (= K202), E278 (= E230), S333 (= S294), W357 (≠ Y318), R377 (= R338), G393 (= G356), E406 (= E369), K410 (= K373)
binding phosphate ion: D347 (= D308), R353 (≠ A314)
binding tryptophan: P240 (≠ G168), F241 (≠ Y169), S242 (≠ G170)

Sites not aligning to the query:

binding phosphate ion: 113, 116
binding tryptophan: 34, 35, 36, 43, 44, 46

5cwaA Structure of anthranilate synthase component i (trpe) from mycobacterium tuberculosis with inhibitor bound (see paper)
36% identity, 50% coverage: 82:373/587 of query aligns to 178:486/505 of 5cwaA

query
sites
5cwaA

W

W

F

N

G

G

L

T

F

D

E

E

G

R

F

V

E

D

T

-

I

W

A

A

T

Y

D

D

V

A

I

V

A

A

L

R

L

L

P

-

D

D

P

V

R

M

G

T

A

A

W

A

I

L

G

G

R

Q

P

P

Q

L

P

P

G

S

I

T

M

V

-

A

-

T

-

F

-

S

-

R

-

P

-

E

-

P

-

R

-

H

R

R

A

A

A

Q

-

R

-

T

-

V

-

E

Y

Y

D

G

A

A

A

I

F

V

A

E

R

Y

V

L

H

V

D

D

W

Q

I

I

E

A

A

A

G

G

D

E

I

A

Y

F

Q
|
Q

A

V

N

V

L

P

S

S

F

Q

R

R

A

F

S

E

V

M

R

D

M

T

L

D

G

V

D

D

P

P

L

I

A

D

A

V

Y

Y

A

R

A

I

I

L

R

R

A

V

R

T

A

N

A

P

A

S

G

P

Y

Y

G

M

G

Y

I

L

V

L

W

Q

T

V

-

P

G

N

D

S

D

D

W

G

L

A

L

V

S

D

F

F

S

S

-

I

-

V

-

G

-

S

P

P

E
|
E

L

A

F

L

F

V

A

T

L

V

R

H

D

E

G

G

E

W

L

A

I

T

A

T

R

H

P

P

M

I

K
x
A

G

G

T

T

T

R

P

W

R

R

G

G

A

R

T

T

P

D

E

D

E

E

D

D

E

V

A

L

R

E

A

K

T

E

L

L

A

L

T

A

D

D

A

D

K

K

Q

E

R

R

A

A

E
|
E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

M

G

R

R

N

N

D

D

L

L

T

G

R

R

I

V

A

C

A

T

P

P

G

G

S

T

V

V

R

R

V

V

P

E

E

D

L

Y

F

S

K

H

V

I

E

E

T

R

Y

Y

P

S

T

H

L

V

H

M

T
x
H

M

L

V

V

S

S

T

T

V

V

S

T

A

G

R

K

L

L

A

G

D

E

G

G

T

R

G

T

I

A

A

L

D

D

V

A

L

V

A

T

A

A

T

C

F

F

P

P

C

A

G

G

S
x
T

I

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

V

L

I

I

D

E

A

E

V

V

E

E

P

K

D

T

A

R

R

R

G

G

P

L

Y
|
Y

T

G

G

G

S

V

I

V

G

G

R

Y

I

L

D

D

P

F

N

A

G

G

D

N

A

A

A

D

F

F

N

A

V

I

A

A

I
|
I

R
|
R

T

T

L

A

H

L

L

M

P

R

R

-

V

-

S

N

E

G

T

T

A

A

S

Y

I

V

G

Q

L
x
A

G
|
G

S

G

G
|
G

V

V

A

A

D

D

S

S

R

N

A

G

G

S

E

Y

E

E

W

Y

D

N

E
|
E

C

A

L

R

A

N

K
|
K

active site: Q248 (= Q139), E301 (= E186), A317 (≠ K202), E345 (= E230), H382 (≠ T267), T409 (≠ S294), Y433 (= Y318), R453 (= R338), G469 (= G356), E482 (= E369), K486 (= K373)
binding 3-{[(1Z)-1-carboxyprop-1-en-1-yl]oxy}-2-hydroxybenzoic acid: Y433 (= Y318), I452 (= I337), A466 (≠ L353), G467 (= G354), K486 (= K373)

1k0gB The crystal structure of aminodeoxychorismate synthase from phosphate grown crystals (see paper)
36% identity, 44% coverage: 113:373/587 of query aligns to 185:407/415 of 1k0gB

query
sites
1k0gB

Q

Q

P

S

G

N

I

M

M

T

R

R

A

E

A

Q

Y

Y

D

G

A

E

A

K

F

F

A

R

R

Q

V

V

H

Q

D

E

W

Y

I

L

E

H

A

S

G

G

D

D

I

C

Y

Y

Q

Q

A

V

N

N

L

L

S

A

F

Q

R

R

A

F

S

H

V

A

R

T

M

Y

L

S

G

G

D

D

P

E

L

W

A

Q

A

A

Y

F

A

L

A

Q

I

L

R

N

A

Q

R

A

A

N

A

R

A

A

G
x
P

Y
x
F

G

S

G

A

I

F

V

L

W

R

T

L

G

E

D

Q

D

G

W

A

L

I

L

L

S

S

F

L

S

S

P

P

E
|
E

L

R

F

F

F

I

A

L

L

C

R

D

D

N

G

S

E

E

L

I

I

Q

A

T

R

R

P

P

M

I

K
|
K

G

G

T

-

P

-

R

-

G

-

A

-

T

-

P

-

E

-

D

-

E

-

A

-

R

-

A

-

T

-

L

-

A

-

T

-

D

-

A

-

K

-

Q

-

R

-

A

A

E
|
E

N

N

L

L

M

M

I

I

V

V

D

D

L

L

M

M

R

R

N

N

D

D

L

I

T

G

R

R

I

V

A

A

A

V

P

A

G

G

S

S

V

V

R

K

V

V

P

P

E

E

L

L

F

-

K

-

V

-

E

-

T

-

Y

-

P

-

T

-

L

-

H

-

T

-

M

L

V

V

S

S

T

T

V

I

S

T

A

A

R

Q

L

L

A

P

D

E

G

Q

T

L

G

H

I

A

A

S

D

D

V

L

L

L

A

R

A

A

T

A

F

F

P

P

C

G

G

G

S
|
S

I

I

T

T

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

V

I

I

I

D
|
D

A

E

V

L

E

E

P

P

D

Q

A
x
R

R

R

G

N

P

A

Y
x
W

T

C

G

G

S

S

I

I

G

G

R

Y

I

L

D

S

P

F

N

C

G

G

D

N

A

M

A

D

F

T

N

S

V

I

A

T

I

I

R
|
R

T

T

L

-

H

-

L

L

P

T

R

A

V

I

S

N

E

G

T

Q

A

I

S

F

I

C

G

S

L

A

G

G

S

G

G
|
G

V

I

V

V

A

A

D

D

S

S

R

Q

A

E

G

E

E

A

E

E

W

Y

D

Q

E
|
E

C

T

L

F

A

D

K
|
K

active site: E258 (= E186), K274 (= K202), E277 (= E230), S330 (= S294), W354 (≠ Y318), R374 (= R338), G390 (= G356), E403 (= E369), K407 (= K373)
binding phosphate ion: D344 (= D308), R350 (≠ A314)
binding tryptophan: P240 (≠ G168), F241 (≠ Y169)

Sites not aligning to the query:

binding phosphate ion: 112, 113, 116
binding tryptophan: 34, 35, 36, 43, 44, 45, 46

7bvdA Anthranilate synthase component i (trpe)[mycolicibacterium smegmatis]
40% identity, 41% coverage: 132:373/587 of query aligns to 241:482/499 of 7bvdA

query
sites
7bvdA

I

I

E

E

A

A

G

G

D

E

I

A

Y

F

Q
|
Q

A

V

N

V

L

P

S

S

F

Q

R

R

A

F

S

E

V

M

R

D

M

T

L

A

G

A

D

D

P

P

L

L

A

D

A

V

Y

Y

A

R

A

I

I

L

R

R

A

V

R

T

A

N

A

P

A

S

G

P

Y

Y

G

M

G

Y

I

L

V

L

W

N

T

I

G

P

D

D

-

A

D

D

W

G

L

G

L

L

S

D

F

F

S

S

-

I

-

V

-

G

-

S

P

P

E
|
E

L

A

F

L

F

V

A

T

L

V

R

K

D

D

G

G

E

R

L

A

I

T

A

T

R

H

P

P

M

I

K
x
A

G

G

T

T

T

R

P

W

R

R

G

-

A

-

T

-

P

-

E

E

D

D

E

V

A

L

R

E

A

K

T

E

L

L

A

L

T

A

D

D

A

E

K

K

Q

E

R

R

A

A

E
|
E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

M

G

R

R

N

N

D

D

L

L

T

G

R

R

I

V

A

C

A

R

P

P

G

G

S

T

V

V

R

R

V

V

P

D

E

D

L

Y

F

S

K

H

V

I

E

E

T

R

Y

Y

P

S

T

H

L

V

H

M

T
x
H

M

L

V

V

S

S

T

T

V

V

S

T

A

G

R

E

L

L

A

A

D

E

G

D

T

K

G

T

I

A

A

L

D

D

V

A

L

V

A

T

A

A

T

C

F

F

P

P

C

A

G

G

S
x
T

I

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

V

L

I

I

D

E

A

E

V

V

E

E

P

K

D

T

A

R

R

R

G

G

P

L

Y
|
Y

T

G

G

G

S

V

I

V

G

G

R

Y

I

L

D

D

P

F

N

A

G

G

D

N

A

A

A

D

F

F

N

A

V

I

A

A

I

I

R
|
R

T

T

L

A

H

L

L

M

P

R

R

-

V

-

S

N

E

G

T

T

A

A

S

Y

I

V

G

Q

L

A

G

G

S

G

G
|
G

V

V

A

A

D

D

S

S

R

N

A

G

G

P

E

Y

E

E

W

Y

D

T

E
|
E

C

A

L

A

A

N

K
|
K

active site: Q248 (= Q139), E301 (= E186), A317 (≠ K202), E341 (= E230), H378 (≠ T267), T405 (≠ S294), Y429 (= Y318), R449 (= R338), G465 (= G356), E478 (= E369), K482 (= K373)

Sites not aligning to the query:

binding pyruvic acid: 93, 94, 100

O94582 Probable anthranilate synthase component 1; Anthranilate synthase component I; EC 4.1.3.27 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
33% identity, 47% coverage: 101:373/587 of query aligns to 194:466/489 of O94582

query
sites
O94582

L

L

P

P

D

E

P

Q

R

K

G

K

A

V

W

H

I

L

G

G

-

Y

R

E

P

A

Q

K

P

S

G

N

I

V

M

G

R

E

A

D

A

G

Y

Y

D

K

A

A

A

F

F

V

A

S

R

N

V

L

H

K

D

E

W

H

I

I

E

F

A

N

G

G

D

D

I

I

Y

F

Q

Q

A

A

N

V

L

P

S

S

F

Q

R

R

A

I

S

A

V

R

R

R

M

T

L

D

G

L

D

H

P

P

L

F

A

N

A

L

Y

Y

A

R

A

H

I

L

R

R

A

T

R

V

A

N

A

P

A

S

G

P

Y

Y

G

M

G

F

I

Y

V

I

W

H

T

C

G

D

D

D

D

F

W

D

L

I

S

G

F

A

S

S

P

P

E

E

L

L

F

L

F

V

A

K

L

S

R

E

D

H

G

G

E

R

L

I

I

I

A

N

R

H

P

P

M

I

K

A

G

G

T

T

T

V

P

P

R

R

G

G

A

K

T

T

P

K

E

E

D

D

E

E

A

A

A

Y

R

A

A

K

T

D

L

L

A

L

T

A

D

S

A

V

K

K

Q

D

R

R

A

A

E

E

N

H

L

V

M

M

I

L

V

V

D

D

L

L

M

A

R

R

N

N

D

D

L

V

T

S

R

R

I

V

A

C

A

D

P

L

G

D

S

T

V

T

R

S

V

V

P

D

E

K

L

L

F

M

K

T

V

I

E

E

T

K

Y

F

P

S

T

H

L

V

H

Q

T

H

M

L

V

V

S

S

T

Q

V

V

S

S

A

G

R

V

L

L

A

R

D

P

G

D

T

K

G

T

I

R

A

F

D

D

V

A

L

F

A

R

A

S

T

I

F

F

P

P

C

A

G

G

S

T

I

V

T
x
S

G

G

A
x
S

P

P

K

K

I

V

R

R

A

A

M

I

E

Q

V

L

I

V

D

Y

A

G

V

L

E

E

P

K

D

E

A

K

R

R

G

G

P

I

Y

Y

T

A

G

G

S

A

I

V

G

G

R

R

I

W

-

G

-

Y

-

E

D

D

P

D

N

N

G

M

D

D

A

T

A

C

F

-

N

-

V

I

A

A

I

I

R

R

T

T

L

M

H

-

L

-

P

-

R

-

V

V

S

Y

E

K

T

D

A

G

S

T

I

V

G

Y

L

L

-

Q

-

A

G

G

S

G

G

G

V

I

V

V

A

F

D

D

S

S

R

D

A

E

G

Q

E

D

E

E

W

Y

D

V

E

E

C

T

L

L

A

N

K

K

S390 (≠ T296) modified: Phosphoserine
S392 (≠ A298) modified: Phosphoserine

Sites not aligning to the query:

488 modified: Phosphoserine

P00898 Anthranilate synthase component 1; AS; ASI; EC 4.1.3.27 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
33% identity, 44% coverage: 120:380/587 of query aligns to 244:509/520 of P00898

query
sites
P00898

A

A

Y

F

D

G

A

A

A

V

F

V

A

R

R

Q

V

L

H

Q

D

K

W

A

I

I

E

R

A

A

G

G

D

E

I

I

Y

F

Q

Q

A

V

N

V

L

P

S

S

F

R

R

R

A

F

S

S

V

L

R

P

M

-

L

C

G

P

D

S

P

P

L

L

A

A

Y

Y

A

Y

A

V

I

L

R

K

A

K

R

S

A
x
N

A
x
P

A

S

G

P

Y

Y

G
x
M

G
x
F

I

F

V

M

W

Q

T

D

G

N

D

D

D

F

W

T

L

L

L

F

S
x
G

F

A

S

S

P

P

E

E

-

S

L

L

F

K

F

Y

A

D

L

A

R

A

D

S

G

R

E

Q

L

I

I

E

A

I

R

Y

P

P

M

I

K

A

G

G

T

T

T

R

P

P

R

R

G

G

-

R

-

A

-

D

A

G

T

T

P

L

E

D

E

R

D

D

E

L

A

D

A

S

R

R

A

I

T

E

L

L

-

D

-

M

A

R

T

T

D

D

A

H

K

K

Q

E

R

L

A

S

E

E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

M

A

R

R

N

N

D

D

L

L

T

A

R

R

I

I

A

C

A

T

P

P

G

G

S

S

V

R

R

Y

V

V

P

A

E

D

L

L

F

T

K

K

V

V

E

D

T

R

Y

Y

P

S

T

Y

L

V

H

M

T

H

M

L

V

V

S

S

T
x
R

V

V

S

V

A

G

R

E

L

L

A

R

D

H

G

D

T

L

G

D

I

A

A

L

D

H

V

A

L

Y

A

R

A

A

T

C

F

M

P

N

C

M

G

G

S

T

I

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

V

L

I

I

D

A

A

D

V

A

E

E

P

G

D

Q

A

R

R

R

G

G

P

S

Y

Y

T

G

G

G

S

A

I

V

G

G

R

Y

I

F

D

T

P

A

N

H

G
|
G

D

D

A

L

A

D

F

T

N
x
C

V

I

A

V

I

I

R

R

T

S

L

A

H

L

L

V

P

E

R

-

V

-

S

N

E

G

T

I

A

A

S

T

I

V

G

Q

L

A

G

G

S

A

G

G

V

I

V

V

A

L

D

D

S

S

R

V

A

P

G

Q

E

S

E

E

W

A

D

D

E

E

C

T

L

R

A

N

K

K

G

A

A

R

F

A

L

V

T

L

R

R

G

A

N288 (≠ A165) mutation to D: Decrease in feedback control by tryptophan.
P289 (≠ A166) mutation to L: Decrease in feedback control by tryptophan.
M293 (≠ G170) mutation to T: Complete loss of feedback control by tryptophan.
F294 (≠ G171) mutation to L: Decrease in feedback control by tryptophan.
G305 (≠ S182) mutation to S: Decrease in feedback control by tryptophan.
R402 (≠ T271) mutation to W: Almost no change in feedback control by tryptophan.
G460 (= G329) mutation to D: Almost no change in feedback control by tryptophan.
C465 (≠ N334) mutation to Y: Complete loss of feedback control by tryptophan. 4-fold decrease of affinity binding for chorismate.

Sites not aligning to the query:

39 E→K: Complete loss of feedback control by tryptophan.
40 binding ; S→F: Complete loss of feedback control by tryptophan.
41 A→V: Decrease in feedback control by tryptophan.
50 binding
128 R→H: Almost no change in feedback control by tryptophan.
174 C→Y: Almost no change in feedback control by tryptophan.
515 H→Y: Almost no change in feedback control by tryptophan.

1i1qA Structure of the cooperative allosteric anthranilate synthase from salmonella typhimurium (see paper)
33% identity, 44% coverage: 120:380/587 of query aligns to 240:505/512 of 1i1qA

query
sites
1i1qA

A

A

Y

F

D

G

A

A

A

V

F

V

A

R

R

Q

V

L

H

Q

D

K

W

A

I

I

E

R

A

A

G

G

D

E

I

I

Y

F

Q
|
Q

A

V

N

V

L

P

S

S

F

R

R

R

A

F

S

S

V

L

R

P

M

C

L

-

G

P

D

S

P

P

L

L

A

A

Y

Y

A

Y

A

V

I

L

R

K

A

K

R

S

A

N

A

P

A

S

G
x
P

Y
|
Y

G
x
M

G

F

I

F

V

M

W

Q

T

D

G

N

D

D

D

F

W

T

L

L

L

F

S

G

F

A

S

S

P

P

E
|
E

-

S

L

L

F

K

F

Y

A

D

L

A

R

A

D

S

G

R

E

Q

L

I

I

E

A

I

R

Y

P

P

M

I

K
x
A

G

G

T

T

T

R

P

P

R

R

G

G

-

R

-

A

-

D

A

G

T

T

P

L

E

D

E

R

D

D

E

L

A

D

A

S

R

R

A

I

T

E

L

L

-

D

-

M

A

R

T

T

D

D

A

H

K

K

Q

E

R

L

A

S

E
|
E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

M

A

R

R

N

N

D

D

L

L

T

A

R

R

I

I

A

C

A

T

P

P

G

G

S

S

V

R

R

Y

V

V

P

A

E

D

L

L

F

T

K

K

V

V

E

D

T

R

Y

Y

P

S

T

Y

L

V

H

M

T
x
H

M

L

V

V

S

S

T

R

V

V

S

V

A

G

R

E

L

L

A

R

D

H

G

D

T

L

G

D

I

A

A

L

D

H

V

A

L

Y

A

R

A

A

T

C

F

M

P

N

C

M

G

G

S
x
T

I

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

V

L

I

I

D

A

A

D

V

A

E

E

P

G

D

Q

A

R

R

R

G

G

P

S

Y
|
Y

T

G

G

G

S

A

I

V

G
|
G

R

Y

I

F

D

T

P

A

N

H

G

G

D

D

A

L

A

D

F

T

N
x
C

V

I

A

V

I

I

R
|
R

T

S

L

A

H

L

L

V

P

E

R

-

V

-

S

N

E

G

T

I

A

A

S

T

I

V

G

Q

L

A

G

G

S

A

G
|
G

V

I

V

V

A

L

D

D

S

S

R

V

A

P

G

Q

E

S

E

E

W

A

D

D

E
|
E

C

T

L

R

A

N

K
|
K

G

A

A

R

F

A

L

V

T

L

R

R

G

A

active site: Q259 (= Q139), E305 (= E186), A323 (≠ K202), E357 (= E230), H394 (≠ T267), T421 (≠ S294), Y445 (= Y318), R465 (= R338), G481 (= G356), E494 (= E369), K498 (= K373)
binding tryptophan: P287 (≠ G168), Y288 (= Y169), M289 (≠ G170), G450 (= G323), C461 (≠ N334)

Sites not aligning to the query:

binding tryptophan: 34, 35, 36, 46

P00897 Anthranilate synthase component 1; AS; ASI; EC 4.1.3.27 from Serratia marcescens (see paper)
32% identity, 44% coverage: 121:380/587 of query aligns to 244:508/519 of P00897

query
sites
P00897

Y

Y

D

G

A

A

A

V

F

V

A

S

R

E

V

L

H

Q

D

E

W

A

I

I

E

R

A

Q

G

G

D

E

I

I

Y

F

Q

Q

A

V

N

V

L

P

S

S

F

R

R

R

A

F

S

S

V

L

R

P

M

C

L

P

G

A

D

-

P

P

L

L

A

G

A

P

Y

Y

A

Q

A

T

I

L

R

K

A

D

R

N

A

N

A

P

A

S

G
x
P

Y
|
Y

G
x
M

G

F

I

F

V

M

W

Q

T

D

G

D

D

D

D

F

W

T

L

L

L

F

S

G

F

A

S

S

P

P

E

E

-

S

-

A

L

L

F

K

F

Y

A

D

L

A

R

G

D

N

G

R

E

Q

L

I

I

E

A

I

R

Y

P

P

M

I

K

A

G

G

T

T

T

R

P

P

R

R

G

G

-

R

-

A

-

D

A

G

T

S

P

L

E

D

E

L

D

D

E

L

A

D

A

S

R

R

A

I

T

E

L

L

A

E

-

M

-

R

T

T

D

D

A

H

K

K

Q

E

R

L

A

A

E
|
E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

M

A

R

R

N

N

D

D

L

L

T

A

R

R

I

I

A

C

A

Q

P

A

G

G

S

S

V

R

R

Y

V

V

P

A

E

D

L

L

F

T

K

K

V

V

E

D

T

R

Y

Y

P

S

T

F

L

V

H

M

T

H

M

L

V

V

S

S

T

R

V

V

S

V

A

G

R

T

L

L

A

R

D

A

G

D

T

L

G

D

I

V

A

L

D

H

V

A

L

Y

A

Q

A

A

T

C

F

M

P

N

C

M

G

G

S

T

I

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

V

L

I

I

D

A

A

A

V

L

E

R

P

S

D

T

A

R

R

R

G

G

P

S

Y

Y

T

G

G

G

S

R

I

V

G

G

R

Y

I

F

D

T

P

A

N

H

G

R

D

H

A

L

A

D

F

T

N

C

V

I

A

V

I

I

R

R

T

S

L

A

H

Y

L

V

P

E

R

D

V

G

S

H

E

R

T

T

A

-

S

-

I

V

G

Q

L

A

G

G

S

A

G

G

V

V

A

Q

D

D

S

S

R

I

A

R

G

R

E

R

E

E

W

A

D

D

E
|
E

C

T

L

R

A

N

K

K

G

A

A

R

F

A

L

V

T

L

R

R

G

A

PYM 290:292 (≠ GYG 168:170) binding
E360 (= E230) binding
E497 (= E369) binding

Sites not aligning to the query:

39 binding

1i7qA Anthranilate synthase from s. Marcescens (see paper)
32% identity, 44% coverage: 121:380/587 of query aligns to 242:506/517 of 1i7qA

query
sites
1i7qA

Y

Y

D

G

A

A

A

V

F

V

A

S

R

E

V

L

H

Q

D

E

W

A

I

I

E

R

A

Q

G

G

D

E

I

I

Y

F

Q
|
Q

A

V

N

V

L

P

S

S

F

R

R

R

A

F

S

S

V

L

R

P

M

C

L

P

G

A

D

-

P

P

L

L

A

G

A

P

Y

Y

A

Q

A

T

I

L

R

K

A

D

R

N

A

N

A

P

A

S

G

P

Y

Y

G

M

G

F

I

F

V

M

W

Q

T

D

G

D

D

D

D

F

W

T

L

L

L

F

S

G

F

A

S

S

P

P

E
|
E

-

S

-

A

L

L

F

K

F

Y

A

D

L

A

R

G

D

N

G

R

E

Q

L

I

I

E

A

I

R

Y

P

P

M

I

K
x
A

G

G

T

T

T

R

P

P

R

R

G

G

-

R

-

A

-

D

A

G

T

S

P

L

E

D

E

L

D

D

E

L

A

D

A

S

R

R

A

I

T

E

L

L

A

E

-

M

-

R

T

T

D

D

A

H

K

K

Q

E

R

L

A

A

E
|
E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

M

A

R

R

N

N

D

D

L

L

T

A

R

R

I

I

A

C

A

Q

P

A

G

G

S

S

V

R

R

Y

V

V

P

A

E

D

L

L

F

T

K

K

V

V

E

D

T

R

Y

Y

P

S

T

F

L

V

H

M

T
x
H

M

L

V

V

S

S

T

R

V

V

S

V

A

G

R

T

L

L

A

R

D

A

G

D

T

L

G

D

I

V

A

L

D

H

V

A

L

Y

A

Q

A

A

T

C

F

M

P

N

C

M

G

G

S
x
T

I

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

V

L

I

I

D

A

A

A

V

L

E

R

P

S

D

T

A

R

R

R

G

G

P

S

Y
|
Y

T

G

G

G

S

R

I

V

G

G

R

Y

I

F

D

T

P

A

N

V

G

R

D

N

A

L

A

D

F

T

N

C

V

I

A

V

I
|
I

R
|
R

T

S

L

A

H

Y

L

V

P

E

R

D

V

G

S

H

E

R

T

T

A

-

S

-

I

V

G

Q

L
x
A

G
|
G

S

A

G
|
G

V

V

A

Q

D

D

S

S

R

I

A

P

G

E

E

R

E

E

W

A

D

D

E
|
E

C

T

L

R

A

N

K
|
K

G

A

A

R

F

A

L

V

T

L

R

R

G

A

active site: Q260 (= Q139), E306 (= E186), A324 (≠ K202), E358 (= E230), H395 (≠ T267), T422 (≠ S294), Y446 (= Y318), R466 (= R338), G482 (= G356), E495 (= E369), K499 (= K373)
binding magnesium ion: E358 (= E230), E495 (= E369)
binding pyruvic acid: Y446 (= Y318), I465 (= I337), R466 (= R338), A479 (≠ L353), G480 (= G354), K499 (= K373)

1i7sA Anthranilate synthase from serratia marcescens in complex with its end product inhibitor l-tryptophan (see paper)
32% identity, 44% coverage: 121:380/587 of query aligns to 236:500/511 of 1i7sA

query
sites
1i7sA

Y

Y

D

G

A

A

A

V

F

V

A

S

R

E

V

L

H

Q

D

E

W

A

I

I

E

R

A

Q

G

G

D

E

I

I

Y

F

Q
|
Q

A

V

N

V

L

P

S

S

F

R

R

R

A

F

S

S

V

L

R

P

M

C

L

P

G

A

D

-

P

P

L

L

A

G

A

P

Y

Y

A

Q

A

T

I

L

R

K

A

D

R

N

A

N

A

P

A

S

G
x
P

Y
|
Y

G
x
M

G

F

I

F

V

M

W

Q

T

D

G

D

D

D

D

F

W

T

L

L

L

F

S

G

F

A

S

S

P

P

E
|
E

-

S

-

A

L

L

F

K

F

Y

A

D

L

A

R

G

D

N

G

R

E

Q

L

I

I

E

A

I

R

Y

P

P

M

I

K
x
A

G

G

T

T

T

R

P

P

R

R

G

G

-

R

-

A

-

D

A

G

T

S

P

L

E

D

E

L

D

D

E

L

A

D

A

S

R

R

A

I

T

E

L

L

A

E

-

M

-

R

T

T

D

D

A

H

K

K

Q

E

R

L

A

A

E
|
E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

M

A

R

R

N

N

D

D

L

L

T

A

R

R

I

I

A

C

A

Q

P

A

G

G

S

S

V

R

R

Y

V

V

P

A

E

D

L

L

F

T

K

K

V

V

E

D

T

R

Y

Y

P

S

T

F

L

V

H

M

T
x
H

M

L

V

V

S

S

T

R

V

V

S

V

A

G

R

T

L

L

A

R

D

A

G

D

T

L

G

D

I

V

A

L

D

H

V

A

L

Y

A

Q

A

A

T

C

F

M

P

N

C

M

G

G

S
x
T

I

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

V

L

I

I

D

A

A

A

V

L

E

R

P

S

D

T

A

R

R

R

G

G

P

S

Y
|
Y

T

G

G

G

S

R

I
x
V

G
|
G

R

Y

I

F

D

T

P

A

N

V

G

R

D

N

A

L

A
x
D

F

T

N
x
C

V

I

A

V

I

I

R
|
R

T

S

L

A

H

Y

L

V

P

E

R

D

V

G

S

H

E

R

T

T

A

-

S

-

I

V

G

Q

L

A

G

G

S

A

G
|
G

V

V

A

Q

D

D

S

S

R

I

A

P

G

E

E

R

E

E

W

A

D

D

E
|
E

C

T

L

R

A

N

K
|
K

G

A

A

R

F

A

L

V

T

L

R

R

G

A

active site: Q254 (= Q139), E300 (= E186), A318 (≠ K202), E352 (= E230), H389 (≠ T267), T416 (≠ S294), Y440 (= Y318), R460 (= R338), G476 (= G356), E489 (= E369), K493 (= K373)
binding tryptophan: P282 (≠ G168), Y283 (= Y169), M284 (≠ G170), V444 (≠ I322), G445 (= G323), D454 (≠ A332), C456 (≠ N334)

Sites not aligning to the query:

binding tryptophan: 35, 36, 37

5jy9B An iron-bound structure of the salicylate synthase irp9 (see paper)
30% identity, 33% coverage: 181:373/587 of query aligns to 225:414/424 of 5jy9B

query
sites
5jy9B

L

L

S

G

F

F

S

S

P

P

E
|
E

L

L

F

V

F

M

A

S

L

V

R

T

D

G

G

N

E

K

L

V

I

V

A

T

R

E

P

P

M

L

K
x
A

G

G

T

T

T

R

P

D

R

R

G

M

A

G

T

N

P

P

E

E

E

H

D

N

E

K

A

A

A

K

R

E

A

A

T

E

L

L

A

L

T

H

D

D

A

S

K

K

Q

E

R

V

A

L

E
|
E

N

H

L

I

M

L

I

S

V

V

D

K

L

E

M

A

R

I

N

A

D

E

L

L

T

E

R

A

I

V

A

C

A

L

P

P

G

G

S

S

V

V

R

V

V

V

P

E

E

D

L

L

F

M

K

S

V

V

E

R

T

Q

Y

R

P

G

T

S

L

V

H

Q

T
x
H

M

L

V

G

S

S

T

G

V

V

S

S

A

G

R

Q

L

L

A

A

D

E

G

N

T

K

G

D

I

A

A

W

D

D

V

A

L

F

A

T

A

V

T

L

F

F

P

P

C

S

G

I

S
x
T

I

A

T

S

G

G

A

I

P

P

K

K

I

N

R

A

A

A

M

L

E

N

V

A

I

I

D

M

A

Q

V

I

E

E

P

K

D

T

A

P

R

R

G

E

P

L

Y
|
Y

T

S

G

G

S

A

I

I

G

L

R

L

I

L

D

D

P

-

N

-

G

-

D

D

A

T

A

R

F

F

N

D

V

A

A

A

I

L

R

V

T

L

L
x
R

H

S

L

V

P

F

R

Q

V

D

S

S

E

Q

T

R

A

C

S

W

I

I

G

Q

L

A

G

G

S

A

G
|
G

V

I

A

A

D

Q

S

S

R

T

A

P

G

E

E

R

E

E

W

L

D

T

E
|
E

C

T

L

R

A

E

K
|
K

active site: E230 (= E186), A246 (≠ K202), E274 (= E230), H311 (≠ T267), T338 (≠ S294), Y362 (= Y318), R381 (≠ L340), G397 (= G356), E410 (= E369), K414 (= K373)
binding fe (ii) ion: E274 (= E230), E410 (= E369)

Sites not aligning to the query:

active site: 183

Query Sequence

>Ga0059261_2227 FitnessBrowser__Korea:Ga0059261_2227
MLGPAPFVLLDDARPDGADARLYRDPVRIVTAQTPQEVQPALDALDAACAEGLHAAGHLA
YEAGHALEPRLRARHSSSPLLWFGLFEGFETIATDDVIALLPDPRGAWIGRPQPGIMRAA
YDAAFARVHDWIEAGDIYQANLSFRASVRMLGDPLAAYAAIRARAAAGYGGIVWTGDDWL
LSFSPELFFALRDGELIARPMKGTTPRGATPEEDEAARATLATDAKQRAENLMIVDLMRN
DLTRIAAPGSVRVPELFKVETYPTLHTMVSTVSARLADGTGIADVLAATFPCGSITGAPK
IRAMEVIDAVEPDARGPYTGSIGRIDPNGDAAFNVAIRTLHLPRVSETASIGLGSGVVAD
SRAGEEWDECLAKGAFLTRGQRQVDLIETMAFDPREGIPLLERHLARMKASAQALGFAFD
RHAARNELQAATFRLRAPSRVRLLLSRNGNVAIGTAPMPAAPDGPVSVAIRARQVAPRDF
RLAHKTSDRSVYPRSAGAFETLYVDAEGYLTEGSFTSIFVERNGRLLTPPLARGALPGVL
RAELIDTGRAAEAELTPADLGNGFFIGNALRGLVSAVTVAEEAPADL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory