SitesBLAST

5ve5A Crystal structure of persulfide dioxygenase rhodanese fusion protein with rhodanese domain inactivating mutation (c314s) from burkholderia phytofirmans in complex with glutathione (see paper)
30% identity, 87% coverage: 20:213/223 of query aligns to 12:194/350 of 5ve5A

query
sites
5ve5A

Q

Q

N

S

S

S

T

T

-

Y

-

T

-

Y

L

L

W

A

C

D

T

S

E

T

T

T

M
x
R

R

E

G

A

A

V

F

L

T

I

D

D

P

P

G

V

G

F

D

E

L

Q

D

V

R

R

L

R

K

D

A

A

-

L

A

I

Q

E

H

L

G

G

V

L

T

H

I

L

E

L

K

Y

L

T

L

I

I

D

T

T

H
|
H

G

V

H
|
H

I

A

D
|
D

H
|
H

C

V

G

T

Q

G

A

A

G

W

M

M

L

L

A

N

K

R

E

R

L

I

G

G

V

-

P

-

I

-

E

-

G

-

P

-

H

-

E

-

A

-

D

S

R

R

F

I

W

A

I

I

S

S

R

A

L

A

D

S

D

-

G

-

R

-

K

-

Y

-

G

G

I

A

D

E

G

G

K

-

P

-

F

-

E

-

P

A

D

D

R

R

W

Y

L

L

V

S

D

H

G

G

D

D

T

K

V

V

T

E

V

F

G

G

N

T

L

R

T

Y

L

L

D

T

V

V

Y

R

H

A

C

T

P

P

G

G

H
|
H

T

T

P

D

G

G

H

C

V

I

V

T

F

L

H

V

H

L

A

D

P

N

S

E

K

T

L

M

A

A

I

F

V

T

G

G

D
|
D

V

C

I

L

F

L

Q

I

G

R

S

G

I

T

G
|
G

R
|
R

T

T

D

D

F

F

P

Q

M

R

G

G

N

D

H

A

Q

H

D

T

L

M

L

F

D

R

A

A

I

V

T

H

G

G

K

Q

L

I

W

F

P

T

L

L

G

P

G

T

D

A

T

C

V

L

F

L

V

Y

P

P

G

A

H
|
H

G

D

Q
x
Y

P

R

-

G

-

L

-

T

-

V

S

T

N

S

F

V

A

G

Q

E

E

E

R

R

T

F

N

N

P

P

active site: R27 (≠ M32), H60 (= H64), H62 (= H66), D64 (= D68), H65 (= H69), H116 (= H139), D135 (= D158), H176 (= H199)
binding fe (iii) ion: H60 (= H64), H116 (= H139), D135 (= D158)
binding glutathione: H116 (= H139), D135 (= D158), G143 (= G166), R144 (= R167), Y178 (≠ Q201)

Sites not aligning to the query:

binding glutathione: 214, 217, 218

3tp9A Crystal structure of alicyclobacillus acidocaldarius protein with beta-lactamase and rhodanese domains
31% identity, 68% coverage: 19:170/223 of query aligns to 13:160/473 of 3tp9A

query
sites
3tp9A

L

L

Q

A

Q

H

N

A

S

S

T

Y

L

L

L

V

W

G

C

C

T

Q

E

E

T

T

M

G

R

E

G

A

A

C

F

V

T

I

D

D

P

P

G

A

G

R

D

D

L

V

D

E

R

P

L

Y

K

L

A

L

A

T

A

A

Q

K

Q

R

H

E

G

G

V

L

T

R

I

I

E

V

K

A

L

A

L

L

I

E

T

T

H
|
H

G

I

H
|
H

I

A

D
|
D

H
x
F

C

V

G

S

Q

G

A

A

G

R

M

E

L

M

A

A

K

D

E

R

L

A

G

G

V

A

P

A

I

I

-

C

-

V

-

S

-

D

E

E

G

G

P

P

H

P

E

E

A

W

D

K

R

S

F

E

W

Y

I

V

S

K

R

A

L

Y

D

-

G

-

R

-

K

-

Y

-

G

-

I

-

D

-

G

-

K

-

P

-

F

-

E

-

P

P

D

H

R

R

W

L

L

L

V

K

D

D

G

G

D

D

T

E

V

L

T

H

V

F

G

G

N

N

L

V

T

R

L

I

D

V

V

V

Y

M

H

H

C

T

P

P

G

G

H
|
H

T

T

P

P

G

E

H

H

V

V

V

S

F

Y

H

L

A

Y

P

D

S

G

K

K

L

T

A

S

-

P

-

D

-

V

-

P

-

M

-

A

-

L

I

F

V

S

G

G

D
|
D

V

F

I

V

F

F

Q

V

G

G

S

D

I

V

G

G

R

R

T

P

D

D

F

L

active site: H58 (= H64), H60 (= H66), D62 (= D68), F63 (≠ H69), H122 (= H139), D148 (= D158)
binding zinc ion: H58 (= H64), H122 (= H139), D148 (= D158)

O95571 Persulfide dioxygenase ETHE1, mitochondrial; Ethylmalonic encephalopathy protein 1; Hepatoma subtracted clone one protein; Sulfur dioxygenase ETHE1; EC 1.13.11.18 from Homo sapiens (Human) (see 4 papers)
29% identity, 73% coverage: 51:213/223 of query aligns to 66:213/254 of O95571

query
sites
O95571

Q

K

Q

E

H

L

G

G

V

L

T

R

I

L

E

L

K

Y

L

A

L

V

I

N

T

T

H
|
H

G

C

H

H

I

A

D

D

H

H

C

I

G

T

Q

G

A

S

G

G

M

L

L

L

A

R

K

S

E

L

L

L

G

-

V

-

P

-

I

-

E

-

G

-

P

-

H

-

E

P

A

G

D

C

R

Q

F

S

W

V

I

I

S

S

R

R

L

L

D

S

D

G

D

A

G

-

R

-

K

-

Y

-

G

-

I

-

D

-

G

-

K

-

P

-

F

-

E

Q

P

A

D

D

R

L

W

H

L

I

V

E

D

D

G

G

D

D

T

S

V

I

T

R

V

F

G

G

N

R

L

F

T

A

L

L

D

E

V

T

Y

R

H

A

C

S

P

P

G

G

H
|
H

T

T

P

P

G

G

H

C

V

V

V

T

F

F

H

V

H

L

A

N

P

D

S

H

K

S

L

M

A

A

I

F

V
x
T

G

G

D
|
D

V

A

I

L

F

L

Q

I

G

R

S

G

I

C

G

G

R

R

T
|
T

D

D

F

F

P

Q

M

Q

G

G

N

C

H

A

Q

K

D

T

L

L

L

Y

D

H

A

S

I

V

T

H

G

E

K

K

L

I

W

F

P

T

L

L

G

P

G

G

D

D

T

C

V

L

F

I

V

Y

P

P

G

A

H

H

G
x
D

-

Y

-

H

-

G

-

F

Q

T

P

V

S

S

N

T

F

V

A

E

Q

E

E

E

R

R

R

T

T

L

N

N

P

P

H79 (= H64) binding
H135 (= H139) binding
T152 (≠ V156) to I: in EE; reduces protein stability, iron content and enzyme activity; dbSNP:rs1317633085
D154 (= D158) binding
T164 (= T168) to K: in EE; reduces protein stability; dbSNP:rs1268640442
D196 (≠ G200) to N: in EE; reduces protein stability and affinity for substrate; dbSNP:rs763799125

Sites not aligning to the query:

1:7 modified: transit peptide, Mitochondrion
55 L → P: in EE; reduces protein stability; dbSNP:rs182983506

4chlB Human ethylmalonic encephalopathy protein 1 (hethe1) (see paper)
29% identity, 73% coverage: 51:213/223 of query aligns to 50:197/237 of 4chlB

query
sites
4chlB

Q

K

Q

E

H

L

G

G

V

L

T

R

I

L

E

L

K

Y

L

A

L

V

I

N

T

T

H
|
H

G

C

H
|
H

I

A

D
|
D

H
|
H

C

I

G

T

Q

G

A

S

G

G

M

L

L

L

A

R

K

S

E

L

L

L

G

-

V

-

P

-

I

-

E

-

G

-

P

-

H

-

E

P

A

G

D

C

R

Q

F

S

W

V

I

I

S

S

R

R

L

L

D

S

D

G

D

A

G

-

R

-

K

-

Y

-

G

-

I

-

D

-

G

-

K

-

P

-

F

-

E

Q

P

A

D

D

R

L

W

H

L

I

V

E

D

D

G

G

D

D

T

S

V

I

T

R

V

F

G

G

N

R

L

F

T

A

L

L

D

E

V

T

Y

R

H

A

C

S

P

P

G

G

H
|
H

T

T

P

P

G

G

H

C

V

V

V

T

F

F

H

V

H

L

A

N

P

D

S

H

K

S

L

M

A

A

I

F

V

T

G

G

D
|
D

V

A

I

L

F

L

Q

I

G

R

S

G

I

C

G

G

R

R

T

T

D

D

F

F

P

Q

M

Q

G

G

N

C

H

A

Q

K

D

T

L

L

L

Y

D

H

A

S

I

V

T

H

G

E

K

K

L

I

W

F

P

T

L

L

G

P

G

G

D

D

T

C

V

L

F

I

V

Y

P

P

G

A

H
|
H

G

D

-

Y

-

H

-

G

-

F

Q

T

P

V

S

S

N

T

F

V

A

E

Q

E

E

E

R

R

R

T

T

L

N

N

P

P

active site: H63 (= H64), H65 (= H66), D67 (= D68), H68 (= H69), H119 (= H139), D138 (= D158), H179 (= H199)
binding fe (iii) ion: H63 (= H64), H119 (= H139), D138 (= D158)

4efzA Crystal structure of a hypothetical metallo-beta-lactamase from burkholderia pseudomallei
31% identity, 79% coverage: 44:219/223 of query aligns to 52:242/295 of 4efzA

query
sites
4efzA

D

D

R

Q

L

L

K

I

A

A

A

R

A

V

Q

A

H

L

G

G

V

A

T

R

I

V

E

R

K

W

L

L

I

E

T

T

H
|
H

G

V

H
|
H

I

A

D
|
D

H
|
H

C

L

G

S

Q

A

A

A

G

P

M

Y

L

L

A

K

K

T

E

R

L

V

G

G

V

G

P

E

I

I

E

A

G

I

P

G

H

R

E

H

A

V

D

T

R

R

F

V

W

Q

-

D

-

V

I

F

S

G

R

K

L

L

D

F

D

N

D

A

G

G

R

P

K

A

Y

F

G

A

I

H

D

D

G

G

K

S

P

Q

F

F

E

-

P

-

D

D

R

R

W

L

L

L

V

D

D

D

G

G

D

D

T

T

V

L

T

A

V

L

G

G

N

A

L

L

T

S

L

I

D

R

V

A

Y

M

H

H

C

T

P

P

G

G

H
|
H

T

T

P

P

G

A

H

C

V

M

V

T

F

Y

-

V

-

T

-

E

-

A

-

H

-

A

H

H

H

D

A

A

P

R

S

D

K

A

L

A

A

A

I

F

V

V

G

G

D
|
D

V

T

I

L

F

F

Q

M

G

P

S

D

I

Y

G

G

-

T

-

A

R

R

T

C

D

D

F

F

P

P

M

G

G

G

N

D

H

A

Q

R

D

S

L

L

L

Y

D

R

A

S

I

I

T

R

G

K

K

V

L

L

W

S

P

L

L

P

G

P

G

A

D

T

T

R

V

L

F

Y

V

M

P

C

G

H

H

D

G

Y

Q

Q

P

P

-

A

-

I

-

Q

-

Y

-

A

S

S

N

T

F

V

A

A

Q

D

E

E

R

L

R

R

T

E

N

N

P

V

F

H

V

I

A

R

D

E

A

G

V

V

active site: H72 (= H64), H74 (= H66), D76 (= D68), H77 (= H69)
binding calcium ion: H72 (= H64), H147 (= H139), D174 (= D158)

4ysbA Crystal structure of ethe1 from myxococcus xanthus (see paper)
27% identity, 83% coverage: 31:216/223 of query aligns to 23:191/225 of 4ysbA

query
sites
4ysbA

T

T

M

R

R

Q

G

A

A

V

F

L

T

I

D

D

P

P

G

V

G

L

D

E

-

Q

L

V

D

D

R

R

L

D

K

L

A

Q

A

M

A

V

Q

A

Q

E

H

L

G

D

V

L

T

T

I

L

E

T

K

H

L

V

L

F

I

D

T

T

H
|
H

G

V

H
|
H

I

A

D
|
D

H
|
H

C

I

G

T

Q

A

A

S

G

G

M

A

L

L

A

R

K

E

E

R

L

T

G

Q

V

A

P

T

I

V

E

V

G

G

P

-

H

-

E

-

A

-

D

-

R

-

F

-

W

-

I

-

S

-

R

-

L

-

D

-

G

-

R

-

K

-

Y

-

G

S

I

V

D

N

G

G

K

A

P

S

F

C

E

-

P

A

D

N

R

V

W

Q

L

V

V

R

D

H

G

G

D

D

T

E

V

V

T

R

V

V

G

G

N

Q

L

L

T

V

L

F

D

Q

V

V

Y

L

H

A

C

T

P

P

G

G

H
|
H

T

T

P

D

G

D

H

S

V

I

V

S

F

Y

H

-

A

-

P

-

S

-

K

-

L

-

A

-

I

L

V

L

G

G

D

D

V

R

I

V

F

F

Q

T

G

G

S
x
D

I

A

-

L

-

V

-

R

-

G

-

N

G

G

R

R

T

T

D

D

F

F

P

Q

M

N

G

G

N

N

H

A

Q

S

D

Q

L

L

L

Y

D

D

A

S

I

L

T

T

G

R

K

V

L

L

W

F

P

T

L

L

G

P

G

D

D

E

T

T

V

L

F

V

V

Y

P

P

G

G

H
|
H

G

D

Q

Y

P

K

-

G

-

R

-

T

-

V

S

T

N

S

F

I

A

A

Q

E

E

E

R

K

R

R

T

H

N

N

P

P

F

R

V

V

A

A

active site: H57 (= H64), H59 (= H66), D61 (= D68), H62 (= H69), H112 (= H139), D129 (≠ S164), H170 (= H199)
binding fe (iii) ion: H57 (= H64), H112 (= H139), D129 (≠ S164)

2gcuA X-ray structure of gene product from arabidopsis thaliana at1g53580 (see paper)
27% identity, 77% coverage: 43:213/223 of query aligns to 40:201/244 of 2gcuA

query
sites
2gcuA

L

V

D

D

R

R

L

D

K

L

A

K

A

L

A

I

Q

D

Q

E

H

L

G

G

V

L

T

K

I

L

E

I

K

Y

L

A

L

M

I

N

T

T

H
|
H

G

V

H
|
H

I

A

D
|
D

H
|
H

C

V

G

T

Q

G

A

T

G

G

M

L

L

L

A

K

K

T

E

K

L

L

-

P

G

G

V

V

P

-

I

-

E

-

G

-

P

-

H

-

E

-

A

-

D

-

R

K

F

S

W

V

I

I

S

S

R

K

L

A

D

S

D

-

G

G

R

S

K

K

Y

-

G

-

I

-

D

-

G

-

K

-

P

-

F

-

E

-

P

A

D

D

R

L

W

F

L

L

V

E

D

P

G

G

D

D

T

K

V

V

T

S

V

I

G

G

N

D

L

I

T

Y

L

L

D

E

V

V

Y

R

H

A

C

T

P

P

G

G

H
|
H

T

T

P

A

G

G

H

C

V

V

V

T

F

Y

-

V

-

T

-

G

-

E

-

G

-

A

H

D

H

Q

A

P

P

Q

S

P

K

R

L

M

A

A

I

F

V

T

G

G

D
|
D

V

A

I

V

F

L

Q

I

G

R

S

G

I

C

G

G

R

R

T

T

D

D

F

F

P

Q

M

E

G

G

N

S

H

S

Q

D

D

Q

L

L

L

Y

D

E

A

S

I

V

T

H

G

S

K

Q

L

I

W

F

P

T

L

L

G

P

G

K

D

D

T

T

V

L

F

I

V

Y

P

P

G

A

H
|
H

G

D

-

Y

-

K

-

G

-

F

Q

E

P

V

S

S

N

T

F

V

A

G

Q

E

E

E

R

M

R

Q

T

H

N

N

P

P

active site: H61 (= H64), H63 (= H66), D65 (= D68), H66 (= H69), H117 (= H139), D142 (= D158), H183 (= H199)
binding fe (ii) ion: H61 (= H64), H117 (= H139), D142 (= D158)

Q9C8L4 Persulfide dioxygenase ETHE1 homolog, mitochondrial; Glyoxalase II; Glx II; Sulfur dioxygenase ETHE1; EC 1.13.11.18 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
27% identity, 77% coverage: 43:213/223 of query aligns to 89:250/294 of Q9C8L4

query
sites
Q9C8L4

L

V

D

D

R

R

L

D

K

L

A

K

A

L

A

I

Q

D

Q

E

H

L

G

G

V

L

T

K

I

L

E

I

K

Y

L

A

L

M

I

N

T

T

H
|
H

G

V

H

H

I

A

D

D

H

H

C

V

G

T

Q

G

A

T

G

G

M

L

L

L

A

K

K

T

E

K

L

L

-

P

G

G

V

V

P

-

I

-

E

-

G

-

P

-

H

-

E

-

A

-

D

-

R

K

F

S

W

V

I

I

S

S

R

K

L

A

D

S

D

-

G

G

R

S

K

K

Y

-

G

-

I

-

D

-

G

-

K

-

P

-

F

-

E

-

P

A

D

D

R

L

W

F

L

L

V

E

D

P

G

G

D

D

T

K

V

V

T

S

V

I

G

G

N

D

L

I

T

Y

L

L

D

E

V

V

Y

R

H

A

C

T

P

P

G

G

H
|
H

T

T

P

A

G

G

H

C

V

V

V

T

F

Y

-

V

-

T

-

G

-

E

-

G

-

A

H

D

H

Q

A

P

P

Q

S

P

K

R

L

M

A

A

I

F

V

T

G

G

D
|
D

V

A

I

V

F

L

Q

I

G

R

S

G

I

C

G

G

R

R

T

T

D

D

F

F

P

Q

M

E

G

G

N

S

H

S

Q

D

D

Q

L

L

L

Y

D

E

A

S

I

V

T

H

G

S

K

Q

L

I

W

F

P

T

L

L

G

P

G

K

D

D

T

T

V

L

F

I

V

Y

P

P

G

A

H

H

G

D

-

Y

-

K

-

G

-

F

Q

E

P

V

S

S

N

T

F

V

A

G

Q

E

E

E

R

M

R

Q

T

H

N

N

P

P

H110 (= H64) binding
H166 (= H139) binding
D191 (= D158) binding

6sg9FL uS3m (see paper)
36% identity, 40% coverage: 124:212/223 of query aligns to 206:294/320 of 6sg9FL

query
sites
6sg9FL

V

I

T

D

V

F

G

G

N

N

L

G

T

V

L

L

D

L

V

Y

Y

Y

-

I

H

F

C

S

P

P

G

G

H
|
H

T

S

P

P

G

G

H

H

V

M

F

L

H

H

H

I

A

P

P

T

S

E

K

R

L

I

A

L

I

F

V

S

G

G

D
|
D

V

L

I

L

F

F

Q

F

G

N

S
x
K

I

V

G

G

R

R

T

V

D

D

F

L

P

P

M

W

G

A

N

T

H

G

Q

V

D

R

L

L

L

A

D

E

A

S

I

L

T

R

G

-

K

L

L

L

W

E

P

A

L

L

G

P

G

D

D

N

T

T

V

V

F

V

V

V

P

P

G

G

H
|
H

G
|
G

Q
x
R

P

M

S

T

N

T

F

L

A

G

Q

R

E

E

R

R

T

E

N

N

binding zinc ion: H222 (= H139), D241 (= D158), D241 (= D158), H281 (= H199)
binding : K247 (≠ S164), G282 (= G200), R283 (≠ Q201)

Sites not aligning to the query:

binding zinc ion: 108, 110, 112, 320, 320
binding : 40, 43, 45, 57, 58, 63, 67, 319

5k0wB Crystal structure of the metallo-beta-lactamase gob-18 from elizabethkingia meningoseptica (see paper)
34% identity, 45% coverage: 46:146/223 of query aligns to 58:163/271 of 5k0wB

query
sites
5k0wB

L

I

K

K

A

A

A

N

A

I

Q

Q

Q

K

H

L

G

G

V

F

T

N

-

Y

-

K

-

D

I

I

E

K

K

I

L

L

I

L

T

T

H
x
Q

G

A

H
|
H

I

Y

D
|
D

H
|
H

C

T

G

G

Q

A

A

L

G

Q

M

D

L

F

A

K

K

T

E

E

L

T

G

-

V

-

P

-

I

-

E

-

G

-

P

-

H

-

E

-

A

A

D

A

R

K

F

F

W

Y

I

V

S

D

R

K

L

A

D

D

D

V

D

D

-

V

-

L

-

R

-

T

-

G

G

G

R

K

-

S

-

D

-

Y

-

E

-

M

-

G

K

K

Y

Y

G

G

I

V

D

T

G

F

K

K

P

P

F

V

E

T

P

P

D

D

R

K

W

T

L

L

V

K

D

D

G

Q

D

D

T

K

V

I

T

K

V

L

G

G

N

N

L

I

T

T

L

L

D

T

V

L

Y

L

H

H

C

H

P

P

G

G

H
|
H

T

T

P

K

G

G

H

S

V

C

V

S

F

F

active site: Q79 (≠ H64), H81 (= H66), D83 (= D68), H84 (= H69), H156 (= H139)
binding zinc ion: Q79 (≠ H64), H81 (= H66), D83 (= D68), H84 (= H69), H156 (= H139)

Sites not aligning to the query:

active site: 188, 222
binding zinc ion: 222

3r2uB 2.1 angstrom resolution crystal structure of metallo-beta-lactamase from staphylococcus aureus subsp. Aureus col
26% identity, 91% coverage: 19:221/223 of query aligns to 15:227/336 of 3r2uB

query
sites
3r2uB

L

L

Q

S

Q

Q

N

A

S

S

T

Y

L

L

L

I

W

G

C

C

T

Q

E

K

T

T

M

G

R

E

G

A

A

M

F

I

T

I

D

D

P

P

G

I

G

R

D

D

L

L

D

S

R

S

L

Y

K

I

A

R

A

V

A

A

Q

D

Q

E

H

E

G

G

V

L

T

T

I

I

E

T

K

H

L

A

I

E

T

T

H
|
H

G

I

H
|
H

I

A

D
|
D

H
x
F

C

A

G

S

Q

G

A

I

G

R

M

D

L

V

A

A

K

I

E

K

L

L

G

N

V

A

P

N

I

I

E

-

G

-

P

-

H

-

E

-

A

-

D

-

R

-

F

-

W

Y

I

V

S

S

R

G

L

E

D

S

D

D

G

-

R

-

K

T

Y

L

G

G

I

Y

D

K

G

N

K

M

P

P

F

-

E

N

P

H

D

T

R

H

W

F

L

V

V

Q

D

H

G

N

D

D

T

D

V

I

T

Y

V

V

G

G

N

N

L

I

T

K

L

L

D

K

V

V

Y

L

H

H

C

T

P

P

G

G

H
|
H

T

T

P

P

G

E

H

S

V

I

V

S

F

F

-

L

-

T

-

D

-

E

-

G

-

A

-

G

H

A

H

Q

A

V

P

P

S

M

K

G

L

L

A

-

I

F

V

S

G

G

D
|
D

V

F

I

I

F

F

Q

V

G

G

S

D

I

I

G

G

R

R

T

P

D

D

F

L

P

S

M

E

G

I

N

G

H

A

Q

K

D

Q

L

M

L

F

D

K

A

S

I

I

T

E

G

S

-

I

-

K

-

D

-

L

-

P

-

D

-

Y

-

I

K

Q

L

I

W

W

P

P

-

G

-
x
H

-

G

-

A

-

G

-

S

L

L

G

G

G

A

D

I

T

P

V

T

F

S

V

T

P

L

G

G

H

Y

G

E

Q

K

P

Q

S

T

N

N

F

W

A

A

Q

F

E

S

R

E

R

N

T

N

N

E

P

A

F

T

V

F

A

I

D

D

A

K

V

L

L

I

A

S

active site: H60 (= H64), H62 (= H66), D64 (= D68), F65 (≠ H69), H123 (= H139), D149 (= D158), H189 (vs. gap)
binding fe (iii) ion: H60 (= H64), H123 (= H139), D149 (= D158)

Sites not aligning to the query:

binding magnesium ion: 304, 328

3r2uA 2.1 angstrom resolution crystal structure of metallo-beta-lactamase from staphylococcus aureus subsp. Aureus col
31% identity, 68% coverage: 19:170/223 of query aligns to 13:159/348 of 3r2uA

query
sites
3r2uA

L

L

Q

S

Q

Q

N

A

S

S

T

Y

L

L

L

I

W

G

C

C

T

Q

E

K

T

T

M

G

R

E

G

A

A

M

F

I

T

I

D

D

P

P

G

I

G

R

D

D

L

L

D

S

R

S

L

Y

K

I

A

R

A

V

A

A

Q

D

Q

E

H

E

G

G

V

L

T

T

I

I

E

T

K

H

L

A

I

E

T

T

H
|
H

G

I

H
|
H

I

A

D
|
D

H
x
F

C

A

G

S

Q

G

A

I

G

R

M

D

L

V

A

A

K

I

E

K

L

L

G

N

V

A

P

N

I

I

E

-

G

-

P

-

H

-

E

-

A

-

D

-

R

-

F

-

W

Y

I

V

S

S

R

G

L

E

D

S

D

D

G

-

R

-

K

T

Y

L

G

G

I

Y

D

K

G

N

K

M

P

P

F

-

E

N

P

H

D

T

R

H

W

F

L

V

V

Q

D

H

G

N

D

D

T

D

V

I

T

Y

V

V

G

G

N

N

L

I

T

K

L

L

D

K

V

V

Y

L

H

H

C

T

P

P

G

G

H
|
H

T

T

P

P

G

E

H

S

V

I

V

S

F

F

-

L

-

T

-

D

-

E

-

G

-

A

-

G

H

A

H

Q

A

V

P

P

S

M

K

G

L

L

A

-

I

F

V

S

G

G

D
|
D

V

F

I

I

F

F

Q

V

G

G

S

D

I

I

G

G

R

R

T

P

D

D

F

L

active site: H58 (= H64), H60 (= H66), D62 (= D68), F63 (≠ H69), H121 (= H139), D147 (= D158)
binding fe (iii) ion: H58 (= H64), H121 (= H139), D147 (= D158)

Sites not aligning to the query:

active site: 197

4ad9A Crystal structure of human lactb2. (see paper)
27% identity, 70% coverage: 46:200/223 of query aligns to 59:200/288 of 4ad9A

query
sites
4ad9A

L

L

K

K

A

Q

A

A

A

L

Q

T

Q

E

H

F

G

N

V

T

T

A

I

I

E

Q

K

E

L

I

L

V

I

V

T

T

H
|
H

G

W

H
|
H

I

R

D
|
D

H
|
H

C

S

G

G

Q

G

A

I

G

G

M

D

L

I

A

C

K

K

E

S

L

I

G

-

V

-

P

-

I

-

E

-

G

-

P

-

H

N

E

N

A

D

D

T

R

T

F

Y

W

C

I

I

S

K

R

K

L

L

D

P

D

R

D

N

G

P

R

Q

K

R

Y

E

G

E

I

I

D

I

G

G

K

N

P

G

F

E

E

Q

P

Q

D

Y

R

V

W

Y

L

L

V

K

D

D

G

G

D

D

T

V

V

I

T

K

V

T

G

E

N

G

L

A

T

T

L

L

D

R

V

V

Y

L

H

Y

C

T

P

P

G

G

H
|
H

T

T

P

D

G

D

H

H

V

M

V

A

F

L

H

L

A

E

P

E

S

E

K

N

L

A

A

I

I

F

V

S

G

G

D
|
D

V

C

I

I

F

L

Q

-

G

-

S

G

I

E

G

G

R

T

T

T

D

V

F

F

P

-

M

-

G

-

N

-

H

-

Q

E

D

D

L

L

L

Y

D

D

A

Y

I

M

T

N

G

S

-

L

-

K

K

E

L

L

W

L

P

K

L

I

G

K

G

A

D

D

T

I

V

I

F

Y

V

-

P

P

G

G

H
|
H

G

G

binding zinc ion: H77 (= H64), H79 (= H66), D81 (= D68), H82 (= H69), H145 (= H139), D164 (= D158), D164 (= D158), H199 (= H199)

Q53H82 Endoribonuclease LACTB2; Beta-lactamase-like protein 2; EC 3.1.27.- from Homo sapiens (Human) (see paper)
27% identity, 70% coverage: 46:200/223 of query aligns to 59:200/288 of Q53H82

query
sites
Q53H82

L

L

K

K

A

Q

A

A

A

L

Q

T

Q

E

H

F

G

N

V

T

T

A

I

I

E

Q

K

E

L

I

L

V

I

V

T

T

H
|
H

G
x
W

H
|
H

I
x
R

D
|
D

H
|
H

C

S

G

G

Q

G

A

I

G

G

M

D

L

I

A

C

K

K

E

S

L

I

G

-

V

-

P

-

I

-

E

-

G

-

P

-

H

N

E

N

A

D

D

T

R

T

F

Y

W

C

I

I

S

K

R

K

L

L

D

P

D

R

D

N

G

P

R

Q

K
x
R

Y

E

G

E

I

I

D

I

G

G

K
x
N

P

G

F
x
E

E

Q

P

Q

D

Y

R

V

W

Y

L

L

V

K

D

D

G

G

D

D

T

V

V

I

T

K

V

T

G

E

N

G

L

A

T

T

L

L

D

R

V

V

Y

L

H

Y

C

T

P

P

G

G

H

H

T

T

P

D

G

D

H

H

V

M

V

A

F

L

H

L

A

E

P

E

S

E

K

N

L

A

A

I

I

F

V

S

G

G

D
|
D

V

C

I

I

F

L

Q

-

G

-

S

G

I

E

G

G

R

T

T

T

D

V

F

F

P

-

M

-

G

-

N

-

H

-

Q

E

D

D

L

L

L

Y

D

D

A

Y

I

M

T

N

G

S

-

L

-

K

K

E

L

L

W

L

P

K

L

I

G

K

G

A

D

D

T

I

V

I

F

Y

V

-

P

P

G

G

H

H

G

G

HWHRDH 77:82 (≠ HGHIDH 64:69) mutation Missing: Loss of endoribonuclease activity.
D81 (= D68) mutation to A: Loss of endoribonuclease activity.
R110 (≠ K104) mutation to A: Mildly decreases endoribonuclease activity. No effect on RNA-binding.
N116 (≠ K110) mutation to A: Loss of endoribonuclease activity. No effect on RNA-binding.
E118 (≠ F112) mutation to A: Loss of endoribonuclease activity. No effect on RNA-binding.
D164 (= D158) mutation to A: Loss of endoribonuclease activity.

Sites not aligning to the query:

216:217 HR→AT: Loss of endoribonuclease activity. Strongly decreases RNA-binding.
220 R→T: Loss of endoribonuclease activity. Strongly decreases RNA-binding.

7zo6A L1 metallo-beta-lactamase in complex with hydrolysed cefoxitin (see paper)
30% identity, 47% coverage: 43:146/223 of query aligns to 62:165/265 of 7zo6A

query
sites
7zo6A

L

L

D

D

R

N

L

M

K

K

A

A

A

-

Q

-

H

R

G

G

V

V

T

T

-

P

-

R

-

D

I

L

E

R

K

L

L

I

L

L

I

L

T

S

H
|
H

G

A

H
|
H

I

A

D
|
D

H
|
H

C

A

G

G

Q

P

A

V

G

A

M

E

L

L

A

K

K

R

E

R

L

T

G

G

V

A

P

K

I

V

E

A

G

A

P

N

H

A

E

E

A

S

D

A

R

V

F

L

W

-

I

L

S

A

R

R

L

G

D

G

D

S

D

D

G

D

R

L

K

H

Y
x
F

G

G

-

D

-

G

I
|
I

D

T

G

Y

K

P

P

P

F

A

E

N

P

A

D

D

R

R

W

I

L

V

V

M

D

D

G

G

D

E

T

V

V

I

T

T

V

V

G

G

N

G

L

I

T

V

L

F

D

T

V

A

Y

H

H

F

C

M

P

A

G

G

H
|
H

T

T

P

P

G

G

H

S

V

T

V

A

F

W

binding (2~{R},5~{S})-5-(aminocarbonyloxymethyl)-2-[(1~{S})-1-methoxy-2-oxidanyl-2-oxidanylidene-1-(2-thiophen-2-ylethanoylamino)ethyl]-5,6-dihydro-2~{H}-1,3-thiazine-4-carboxylic acid: D86 (= D68), F122 (≠ Y105), I126 (= I107), H158 (= H139)
binding zinc ion: H82 (= H64), H84 (= H66), D86 (= D68), H87 (= H69), H158 (= H139)

Sites not aligning to the query:

Query Sequence

>Ga0059261_2228 FitnessBrowser__Korea:Ga0059261_2228
MNTASTPPLRAAIVPVTPLQQNSTLLWCTETMRGAFTDPGGDLDRLKAAAQQHGVTIEKL
LITHGHIDHCGQAGMLAKELGVPIEGPHEADRFWISRLDDDGRKYGIDGKPFEPDRWLVD
GDTVTVGNLTLDVYHCPGHTPGHVVFHHAPSKLAIVGDVIFQGSIGRTDFPMGNHQDLLD
AITGKLWPLGGDTVFVPGHGQPSNFAQERRTNPFVADAVLARS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory