SitesBLAST
Comparing Ga0059261_2257 FitnessBrowser__Korea:Ga0059261_2257 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
37% identity, 99% coverage: 2:521/526 of query aligns to 3:451/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G10), G13 (= G12), S14 (= S13), A15 (≠ G14), E35 (= E34), A36 (= A35), W47 (= W59), P65 (= P77), G67 (= G79), V180 (= V213), A214 (≠ G247), G215 (= G248), A218 (≠ G251), T270 (≠ S330), Y391 (= Y461), A424 (= A494), I435 (≠ T505), N436 (= N506)
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
35% identity, 99% coverage: 3:522/526 of query aligns to 13:528/532 of 4mjwA
- active site: I333 (≠ A335), P377 (≠ A376), N378 (≠ C377), V464 (≠ I460), H466 (= H462), V509 (≠ G503), N510 (= N504)
- binding flavin-adenine dinucleotide: G20 (= G10), G22 (= G12), S23 (= S13), E44 (= E34), A45 (= A35), W71 (= W59), R89 (= R78), A90 (≠ G79), G95 (= G84), C96 (≠ S85), H99 (≠ I88), N100 (= N89), S101 (≠ A90), I103 (≠ L92), R231 (≠ V212), A232 (≠ V213), T269 (≠ G247), G270 (= G248), D273 (≠ G251), Y465 (= Y461), H466 (= H462), A500 (= A494), N510 (= N504), P511 (≠ T505), N512 (= N506), V515 (≠ S509)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
35% identity, 99% coverage: 3:521/526 of query aligns to 13:527/527 of 2jbvA
- active site: I333 (≠ A335), P377 (≠ A376), N378 (≠ C377), V464 (≠ I460), H466 (= H462), V509 (≠ G503), N510 (= N504)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G12), S23 (= S13), E44 (= E34), A45 (= A35), W71 (= W59), A90 (≠ G79), G95 (= G84), C96 (≠ S85), H99 (≠ I88), N100 (= N89), S101 (≠ A90), I103 (≠ L92), R231 (≠ V212), A232 (≠ V213), T269 (≠ G247), G270 (= G248), D273 (≠ G251), V464 (≠ I460), Y465 (= Y461), H466 (= H462), D499 (= D493), A500 (= A494), N510 (= N504), P511 (≠ T505), N512 (= N506), V515 (≠ S509)
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
35% identity, 99% coverage: 3:522/526 of query aligns to 13:528/530 of 3ljpA
- active site: I333 (≠ A335), P377 (≠ A376), N378 (≠ C377), A464 (≠ I460), H466 (= H462), V509 (≠ G503), N510 (= N504)
- binding dihydroflavine-adenine dinucleotide: G22 (= G12), S23 (= S13), E44 (= E34), A45 (= A35), W71 (= W59), R89 (= R78), A90 (≠ G79), G95 (= G84), C96 (≠ S85), H99 (≠ I88), N100 (= N89), S101 (≠ A90), I103 (≠ L92), A232 (≠ V213), T269 (≠ G247), D273 (≠ G251), Y465 (= Y461), H466 (= H462), D499 (= D493), A500 (= A494), N510 (= N504), P511 (≠ T505), N512 (= N506), V515 (≠ S509)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
32% identity, 99% coverage: 5:523/526 of query aligns to 3:564/565 of 5oc1A
- active site: V339 (≠ G315), N413 (≠ A376), A414 (≠ C377), I499 (= I460), H501 (= H462), A544 (≠ G503), H545 (≠ N504)
- binding 4-methoxybenzoic acid: Y91 (≠ A90), I356 (≠ F332), I390 (≠ A357), F396 (≠ G363), T412 (≠ H375), I499 (= I460), H501 (= H462), H545 (≠ N504)
- binding flavin-adenine dinucleotide: G8 (= G10), G10 (= G12), N11 (≠ S13), A12 (≠ G14), E32 (= E34), A33 (= A35), W60 (= W59), P78 (= P77), G80 (= G79), G85 (= G84), S86 (= S85), H90 (≠ N89), Y91 (≠ A90), V93 (≠ L92), V230 (= V213), S270 (≠ A246), A271 (≠ G247), G272 (= G248), F500 (≠ Y461), H545 (≠ N504), T546 (= T505), Q547 (≠ N506), I550 (≠ S509)
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
32% identity, 99% coverage: 5:523/526 of query aligns to 3:564/565 of 3fimB
- active site: V339 (≠ G315), N413 (≠ A376), A414 (≠ C377), I499 (= I460), H501 (= H462), A544 (≠ G503), H545 (≠ N504)
- binding flavin-adenine dinucleotide: G8 (= G10), N11 (≠ S13), A12 (≠ G14), E32 (= E34), A33 (= A35), W60 (= W59), P78 (= P77), G80 (= G79), G85 (= G84), S86 (= S85), H90 (≠ N89), Y91 (≠ A90), V93 (≠ L92), V230 (= V213), S270 (≠ A246), A271 (≠ G247), F500 (≠ Y461), H501 (= H462), H545 (≠ N504), T546 (= T505), Q547 (≠ N506), I550 (≠ S509)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
34% identity, 99% coverage: 2:520/526 of query aligns to 22:569/578 of 5nccA
- active site: R347 (≠ L314), L420 (≠ A376), I421 (≠ C377), S507 (≠ I460), A509 (≠ H462), G552 (= G503), Q553 (≠ N504)
- binding flavin-adenine dinucleotide: G30 (= G10), G32 (= G12), T33 (≠ S13), A34 (≠ G14), L53 (= L33), E54 (= E34), A55 (= A35), F74 (≠ M52), W80 (= W59), A98 (≠ P77), G100 (= G79), G105 (= G84), S106 (= S85), N110 (= N89), A111 (= A90), T112 (≠ M91), L113 (= L92), V238 (= V213), A278 (≠ G247), H282 (≠ G251), L286 (= L255), N508 (≠ Y461), Q553 (≠ N504), T554 (= T505), G555 (≠ N506), V558 (≠ S509)
6yrvAAA structure of fap after illumination at 100k (see paper)
34% identity, 99% coverage: 2:520/526 of query aligns to 6:560/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ H353), N499 (≠ Y461)
- binding flavin-adenine dinucleotide: G14 (= G10), G16 (= G12), T17 (≠ S13), A18 (≠ G14), L37 (= L33), E38 (= E34), A39 (= A35), F58 (≠ M52), W64 (= W59), A82 (≠ P77), G89 (= G84), S90 (= S85), N94 (= N89), A95 (= A90), T96 (≠ M91), L97 (= L92), M191 (≠ T185), V222 (= V213), C264 (≠ A246), A265 (≠ G247), G266 (= G248), H269 (≠ G251), N499 (≠ Y461), A534 (= A494), Q544 (≠ N504), T545 (= T505), G546 (≠ N506)
- binding heptadecane: V377 (≠ L355), G379 (≠ A357), M380 (≠ I358), G386 (vs. gap), T389 (≠ D361), Y390 (≠ H362), F393 (vs. gap), T408 (≠ S373), Q410 (≠ H375)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
34% identity, 99% coverage: 2:520/526 of query aligns to 82:636/654 of A0A248QE08
- TA 93:94 (≠ SG 13:14) binding
- E114 (= E34) binding
- L162 (≠ G81) binding
- S166 (= S85) binding
- NATL 170:173 (≠ NAML 89:92) binding
- V298 (= V213) binding
- C432 (≠ A333) binding
- R451 (≠ H353) binding
- Y466 (≠ H362) binding
- Q486 (≠ H375) binding
- G622 (≠ N506) binding
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
34% identity, 98% coverage: 3:517/526 of query aligns to 1:501/508 of 4ha6A
- active site: F360 (≠ A376), G361 (≠ C377), H444 (≠ I460), H446 (= H462), G487 (= G503), P488 (≠ N504)
- binding flavin-adenine dinucleotide: G8 (= G10), G10 (= G12), S11 (= S13), A12 (≠ G14), E32 (= E34), A33 (= A35), W58 (= W59), R77 (= R78), G78 (= G79), G83 (= G84), S84 (= S85), L87 (≠ I88), H88 (≠ N89), A89 (= A90), M90 (= M91), G91 (≠ L92), V218 (= V213), A251 (≠ G247), G252 (= G248), E255 (≠ G251), H445 (≠ Y461), A478 (= A494), P488 (≠ N504), I489 (≠ T505), H490 (≠ N506)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (= A90), S314 (≠ A333), H444 (≠ I460), H446 (= H462)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
34% identity, 98% coverage: 3:517/526 of query aligns to 1:501/509 of 3t37A
- active site: F360 (≠ A376), G361 (≠ C377), H444 (≠ I460), H446 (= H462), G487 (= G503), P488 (≠ N504)
- binding flavin-adenine dinucleotide: G8 (= G10), G10 (= G12), S11 (= S13), A12 (≠ G14), E32 (= E34), A33 (= A35), W58 (= W59), R77 (= R78), G78 (= G79), R79 (≠ K80), G83 (= G84), S84 (= S85), H88 (≠ N89), A89 (= A90), G91 (≠ L92), R217 (≠ V212), V218 (= V213), A251 (≠ G247), E255 (≠ G251), H445 (≠ Y461), A478 (= A494), P488 (≠ N504), I489 (≠ T505), H490 (≠ N506)
8bxlB Patulin synthase from penicillium expansum
30% identity, 98% coverage: 5:517/526 of query aligns to 15:583/590 of 8bxlB
- binding flavin-adenine dinucleotide: G20 (= G10), G22 (= G12), T23 (≠ S13), A24 (≠ G14), E44 (= E34), A45 (= A35), W80 (= W59), G100 (= G79), G105 (= G84), S106 (= S85), R109 (≠ I88), N110 (= N89), Y111 (≠ A90), A113 (≠ L92), L253 (≠ V212), A254 (≠ V213), A288 (≠ G247), Q292 (≠ G251), F525 (≠ Y461), D559 (= D493), A560 (= A494), H570 (≠ N504), P571 (≠ T505), Q572 (≠ N506), L575 (≠ S509)
5i68A Alcohol oxidase from pichia pastoris (see paper)
28% identity, 99% coverage: 2:524/526 of query aligns to 4:635/662 of 5i68A
- active site: H566 (= H462)
- binding flavin-adenine dinucleotide: G13 (≠ A11), G14 (= G12), S15 (= S13), S16 (≠ G14), E37 (= E34), A38 (= A35), M58 (≠ I50), A86 (≠ G79), G91 (= G84), G92 (≠ S85), N96 (= N89), F97 (≠ A90), M99 (≠ L92), K225 (≠ V212), V226 (= V213), C267 (≠ G247), G268 (= G248), S271 (≠ G251), W565 (vs. gap), L605 (≠ A494), N615 (= N504), T616 (= T505), Y617 (≠ N506)
5hsaA Alcohol oxidase aox1 from pichia pastoris (see paper)
28% identity, 99% coverage: 2:524/526 of query aligns to 4:635/662 of 5hsaA
- active site: H566 (= H462)
- binding arabino-flavin-adenine dinucleotide: G14 (= G12), S15 (= S13), S16 (≠ G14), E37 (= E34), A38 (= A35), A86 (≠ G79), G92 (≠ S85), N96 (= N89), F97 (≠ A90), M99 (≠ L92), K225 (≠ V212), V226 (= V213), C267 (≠ G247), S271 (≠ G251), W565 (vs. gap), L605 (≠ A494), N615 (= N504), T616 (= T505), Y617 (≠ N506)
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
32% identity, 98% coverage: 5:520/526 of query aligns to 3:523/525 of 4udqA
- active site: L331 (= L314), F364 (≠ I367), W365 (≠ C377), V461 (≠ I460), H463 (= H462), A506 (≠ G503), N507 (= N504)
- binding flavin-adenine dinucleotide: G8 (= G10), G10 (= G12), T11 (≠ S13), A12 (≠ G14), E32 (= E34), A33 (= A35), W64 (≠ A56), G88 (= G79), G93 (= G84), G94 (≠ S85), N98 (= N89), M99 (≠ A90), V101 (≠ L92), V229 (= V213), T261 (≠ A246), A262 (≠ G247), W462 (≠ Y461), H463 (= H462), A497 (= A494), N507 (= N504), T508 (= T505), N509 (= N506), T512 (≠ S509)
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
32% identity, 98% coverage: 5:520/526 of query aligns to 7:527/531 of E4QP00
- V101 (≠ I88) mutation to H: Abolishes activity.
- M103 (≠ A90) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (≠ K366) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ C377) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (≠ I460) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ Y461) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H462) mutation to A: Abolishes activity.
- N511 (= N504) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
3q9tA Crystal structure analysis of formate oxidase (see paper)
29% identity, 99% coverage: 5:523/526 of query aligns to 8:572/577 of 3q9tA
- active site: A335 (≠ G315), D422 (vs. gap), A508 (≠ I460), H510 (= H462), C552 (≠ G503), R553 (≠ N504)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl(2R,3S,4S)-5-(8-formyl-7-methyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl)-2,3,4-trihydroxypentyl dihydrogendiphosphate: G15 (= G12), T16 (≠ S13), E37 (= E34), A38 (= A35), W65 (= W59), T85 (vs. gap), R86 (= R78), G87 (= G79), G92 (= G84), S93 (= S85), N97 (= N89), Y98 (≠ A90), F99 (≠ M91), T100 (≠ L92), S229 (≠ V213), S265 (≠ A246), Q266 (≠ G247), E270 (≠ G251), F509 (≠ Y461), D542 (= D493), A543 (= A494), R553 (≠ N504), I554 (≠ T505), Q555 (≠ N506), V558 (≠ S509)
5zu2A Effect of mutation (r554a) on fad modification in aspergillus oryzae rib40formate oxidase (see paper)
29% identity, 99% coverage: 5:523/526 of query aligns to 8:572/577 of 5zu2A
- binding flavin-adenine dinucleotide: G15 (= G12), T16 (≠ S13), E37 (= E34), A38 (= A35), W65 (= W59), T85 (vs. gap), R86 (= R78), G87 (= G79), G92 (= G84), S93 (= S85), N97 (= N89), Y98 (≠ A90), T100 (≠ L92), S229 (≠ V213), S265 (≠ A246), Q266 (≠ G247), E270 (≠ G251), F509 (≠ Y461), D542 (= D493), A543 (= A494), A553 (≠ N504), I554 (≠ T505), Q555 (≠ N506), V558 (≠ S509)
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
30% identity, 98% coverage: 3:520/526 of query aligns to 15:572/577 of 4h7uA
- active site: A343 (≠ G315), V426 (vs. gap), Y510 (≠ I460), H512 (= H462), A555 (≠ G503), H556 (≠ N504)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G10), G24 (= G12), T25 (≠ S13), A26 (≠ G14), E46 (= E34), A47 (= A35), W74 (= W59), G99 (= G84), C100 (≠ S85), H103 (≠ I88), N104 (= N89), G105 (≠ A90), V107 (≠ L92), L242 (≠ V212), V243 (= V213), G282 (= G247), G283 (= G248), A286 (≠ G251), H512 (= H462), A546 (= A494), H556 (≠ N504), T557 (= T505), Q558 (≠ N506), V561 (≠ S509)
Q3L245 Pyranose dehydrogenase 1; PDH 1; Pyranose:quinone oxidoreductase 1; EC 1.1.99.29 from Leucoagaricus meleagris (Western flat-topped agaric) (Agaricus meleagris) (see 2 papers)
30% identity, 98% coverage: 3:520/526 of query aligns to 40:597/602 of Q3L245
- N100 (≠ A60) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H128 (≠ I88) modified: Tele-8alpha-FAD histidine
- N344 (= N284) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H537 (= H462) active site, Proton acceptor
- H581 (≠ N504) active site
Sites not aligning to the query:
Query Sequence
>Ga0059261_2257 FitnessBrowser__Korea:Ga0059261_2257
MEEADIVVIGAGSGGSAAAGRLSEDGKYSVAVLEAGGRNTGFRTLMPGAIAMQTPATNWA
YETVPQPGLNGRRGYQPRGKGLGGSSAINAMLYIRGNPWDYDNWEALGASGWGWADVLPV
FKRSETNQRGASQWHGGDGPLQVSDQSFVHPGSQAFVDAAASLQIPRNDDFNGVRQEGAG
IYQVTQKDGERWTAARGYLTPRPNLEILCDTVVERVLFEDGRAWGVAYSRGGQSKMIRAR
RAVVLAGGVFGTAQLLMLSGIGPGAHLQEHGLTVRIDRGEVGGNLQDHLDYVAAFETKGT
QFLGRSLIGSLKGLGAMARWFRTRSGPMTSPFAEAGAFLRTRPELPAPDIQLHFLVAIVE
DHGRAKIKTHGYSCHACVLRPESRGTVRLASPDPRAAPLIDPGFLTDRRDVDLLIKGVRA
MYRILDAEPLTAFGGRDRYPLDRNDDAALERLIRARADTIYHPVGTARMGSDDRAVCDPK
LRVRGVDGLYVADASVMPRLISGNTNAPSIMIGERCADFIRADLSE
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory