SitesBLAST – Find functional sites

 

SitesBLAST

Comparing Ga0059261_2298 FitnessBrowser__Korea:Ga0059261_2298 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

5t9fB Prephenate dehydrogenase n222d mutant from soybean (see paper)
31% identity, 89% coverage: 3:223/249 of query aligns to 4:232/253 of 5t9fB

query
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5t9fB
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Q

2pv7B Crystal structure of chorismate mutase / prephenate dehydrogenase (tyra) (1574749) from haemophilus influenzae rd at 2.00 a resolution (see paper)
32% identity, 67% coverage: 8:173/249 of query aligns to 17:170/280 of 2pv7B

query
sites
2pv7B
G
 
G
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Y
G
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G
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F

Sites not aligning to the query:

4wjiA Crystal structure of cyclohexadienyl dehydrogenase from sinorhizobium meliloti in complex with NADP and tyrosine
31% identity, 72% coverage: 1:180/249 of query aligns to 4:200/293 of 4wjiA

query
sites
4wjiA
M
 
F
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G
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S
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G
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A
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E
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I
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A
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L
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A
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G
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D
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E
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E
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I
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I
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Y
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N
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I
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V
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G

Sites not aligning to the query:

2f1kA Crystal structure of synechocystis arogenate dehydrogenase (see paper)
25% identity, 71% coverage: 3:180/249 of query aligns to 2:190/279 of 2f1kA

query
sites
2f1kA
R
 
K
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G
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V
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G
Q
 
A
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L
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A
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G
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D
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L
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R
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R
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G
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H
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C
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D
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S
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P
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A
E
 
D
H
 
H
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D
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Q
E
 
A
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A
Y
 
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A
 
H
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P
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M
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V
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A
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A
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L
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I
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Q

Query Sequence

>Ga0059261_2298 FitnessBrowser__Korea:Ga0059261_2298
MKRFGIIGFGRFGQLAARHLRDHFSVVVTDTADVGEAAAAIGVKTGSLADAADCDVVMLA
VPVQAMAATIAAIAPLVRPGALVLDVASVKMLPARWMLEALPESVDIVATHPLFGPQSAR
GGLEGQPLVVCAVRGERHHKVAEFGRSLGLSVSITTAEEHDREMAYVQALTHLIGRALVN
IRIPDEELKTNSYQHLLELCGLIRDDSKELFFAIQNLNPYAEEITRQFIAEANGLLAESL
KAGNRSAAD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory