SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ga0059261_2801 FitnessBrowser__Korea:Ga0059261_2801 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
41% identity, 99% coverage: 1:251/253 of query aligns to 4:246/247 of 3op4A

query
sites
3op4A

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binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), S17 (≠ A14), R18 (≠ Q15), I20 (= I17), T40 (≠ R37), N62 (≠ S60), V63 (= V61), N89 (= N87), A90 (= A88), I92 (= I90), V139 (≠ I143), S141 (= S145), Y154 (= Y158), K158 (= K162), P184 (≠ Y188), G185 (= G189), I187 (≠ V191), T189 (= T193), M191 (= M195)

4nbuB Crystal structure of fabg from bacillus sp (see paper)
41% identity, 97% coverage: 3:248/253 of query aligns to 5:241/244 of 4nbuB

query
sites
4nbuB

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active site: G18 (= G16), N111 (= N111), S139 (= S145), Q149 (= Q155), Y152 (= Y158), K156 (= K162)
binding acetoacetyl-coenzyme a: D93 (≠ T93), K98 (= K98), S139 (= S145), N146 (≠ T152), V147 (≠ I153), Q149 (= Q155), Y152 (= Y158), F184 (≠ I190), M189 (= M195), K200 (≠ R207)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G12), N17 (≠ Q15), G18 (= G16), I19 (= I17), D38 (= D36), F39 (≠ R37), V59 (≠ I57), D60 (≠ S60), V61 (= V61), N87 (= N87), A88 (= A88), G89 (= G89), I90 (= I90), T137 (≠ I143), S139 (= S145), Y152 (= Y158), K156 (= K162), P182 (≠ Y188), F184 (≠ I190), T185 (≠ V191), T187 (= T193), M189 (= M195)

4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
40% identity, 99% coverage: 1:251/253 of query aligns to 4:242/243 of 4i08A

query
sites
4i08A

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active site: G19 (= G16), N113 (= N111), S141 (= S145), Q151 (= Q155), Y154 (= Y158), K158 (= K162)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), S17 (≠ A14), R18 (≠ Q15), I20 (= I17), T40 (≠ R37), N62 (≠ S60), V63 (= V61), N89 (= N87), A90 (= A88), G140 (≠ S144), S141 (= S145), Y154 (= Y158), K158 (= K162), P184 (≠ Y188), G185 (= G189), T189 (= T193)

P73826 Acetoacetyl-CoA reductase; EC 1.1.1.36 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
39% identity, 99% coverage: 1:250/253 of query aligns to 4:239/240 of P73826

query
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P73826

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S134 (= S145) mutation to A: 12% enzymatic activity.
Y147 (= Y158) mutation to A: No enzymatic activity.
K151 (= K162) mutation to A: 5% enzymatic activity.

3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
40% identity, 97% coverage: 6:251/253 of query aligns to 5:246/246 of 3osuA

query
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3osuA

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G

G

Q
|
Q

I

A

N

N

Y
|
Y

G

V

A

A

A

T

K
|
K

S

A

G

G

V

V

L

I

G

G

L

L

T

T

M

K

S

S

A

A

R

R

E

E

W

L

G

A

R

S

Y

R

G

G

I

I

R

T

T

V

N

N

S

A

V

V

A

A

Y

P

G

G

I

F

V

I

E

V

T

S

D

D

M

M

T

T

E

D

T

A

V

L

R

-

S

S

E

D

K

E

F

L

R

K

D

E

R

Q

Y

M

L

L

A

T

N

Q

I

I

P

P

L

L

G

A

R

R

F

F

L

G

T

Q

K

D

E

T

E

D

A

I

A

A

N

N

S

T

I

V

A

A

F

F

L

L

L

A

S

S

P

D

A

K

A

A

A

K

F

Y

I

I

T

T

G

G

Q

Q

H

T

L

I

S

H

V

V

N

N

G

G

S

M

H

Y

I

M

active site: G15 (= G16), S141 (= S145), Q151 (= Q155), Y154 (= Y158), K158 (= K162)
binding magnesium ion: N89 (= N87), K158 (= K162)

3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
40% identity, 97% coverage: 6:251/253 of query aligns to 2:239/239 of 3sj7A

query
sites
3sj7A

R

K

T

S

I

A

L

L

I

V

T

T

G
|
G

A

A

A
x
S

Q
x
R

G
|
G

I
|
I

G

G

L

R

A

S

T

I

A

A

Q

L

L

Q

C

L

A

A

A

E

L

E

D

G

A

Y

N

N

V

V

-

A

I

V

L
x
N

L
x
Y

D
x
A

R
x
G

S
|
S

E

K

E

E

Q

K

L

A

D

E

A

A

A

V

L

V

A

E

S

E

F

I

D

K

S

A

G

K

-

G

-

V

K

D

A

S

M

F

K

A

I

I

A

Q

G
x
A

S
x
N

V
|
V

T

A

D

D

R

A

A

D

F

E

V

V

K

K

Q

A

A

M

V

I

A

K

E

E

A

V

A

S

R

Q

F

F

G

G

G

S

I

L

H

D

G

V

L

L

V

V

N

N

N
|
N

A
|
A

G

G

I

I

T

T

R

R

T

D

A

N

M

L

I

L

D

M

K

R

M

M

T

K

A

E

D

Q

D

E

W

W

Q

D

A

D

V

V

I

I

D

D

V
x
T

N

N

L

L

T

K

G

G

A

V

F

F

N

N

M

C

L

I

Q

Q

A

K

V

A

G

T

M

P

D

Q

M

M

I

L

A

-

R

R

A

Q

K

R

S

S

G

G

E

-

S

-

D

-

P

-

G

-

A

A

I

I

V

I

N

N

I

L

S

S

S
|
S

D

V

A

V

G

G

R

A

K

V

G

G

T

N

I

P

G

G

Q
|
Q

I

A

N

N

Y
|
Y

G

V

A

A

A

T

K
|
K

S

A

G

G

V

V

L

I

G

G

L

L

T

T

M

K

S

S

A

A

R

R

E

E

W

L

G

A

R

S

Y

R

G

G

I

I

R

T

T

V

N

N

S

A

V

V

A

A

Y
x
P

G
|
G

I

F

V

I

E

V

T

S

D

D

M

M

T

T

E

-

T

-

V

-

R

-

S

-

E

D

K

E

F

L

R

K

D

E

R

Q

Y

M

L

L

A

T

N

Q

I

I

P

P

L

L

G

A

R

R

F

F

L

G

T

Q

K

D

E

T

E

D

A

I

A

A

N

N

S

T

I

V

A

A

F

F

L

L

L

A

S

S

P

D

A

K

A

A

A

K

F

Y

I

I

T

T

G

G

Q

Q

H

T

L

I

S

H

V

V

N

N

G

G

S

M

H

Y

I

M

active site: G12 (= G16), S138 (= S145), Q148 (= Q155), Y151 (= Y158), K155 (= K162)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), S10 (≠ A14), R11 (≠ Q15), I13 (= I17), N31 (≠ L34), Y32 (≠ L35), A33 (≠ D36), G34 (≠ R37), S35 (= S38), A58 (≠ G59), N59 (≠ S60), V60 (= V61), N86 (= N87), A87 (= A88), T109 (≠ V110), S138 (= S145), Y151 (= Y158), K155 (= K162), P181 (≠ Y188), G182 (= G189)

P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
42% identity, 97% coverage: 3:248/253 of query aligns to 4:243/247 of P73574

query
sites
P73574

L

L

S

T

G

A

R

Q

T

V

I

A

L

L

I

V

T

T

G

G

A
|
A

A

S

Q

R

G

G

I

I

G

G

L

K

A

A

T

T

A

A

Q

L

L

A

C

L

A

A

L

T

D

G

A

M

N

K

V

V

I

V

L

V

-

N

L

Y

D

A

R

Q

S

S

E

S

E

T

Q

A

L

A

D

D

A

A

A

V

L

V

A

A

S

E

F

I

-

I

-

A

D

N

S

G

G

G

K

E

A

A

M

I

K

A

I

V

A

Q

G

A

S

N

V

V

T

A

D

N

R

A

A

D

F

E

V

V

K

D

Q

Q

A

L

V

I

A

K

E

T

A

T

A

L

A

D

R

K

F

F

G

S

G

R

I

I

H

D

G

V

L

L

V

V

N

N

A

A

G

G

I

I

T

T

R

R

T

D

A

T

M

L

I

L

D

L

K

R

M

M

T

K

A

L

D

E

D

D

W

W

Q

Q

A

A

V

V

I

I

D

D

V

L

N

N

L

L

T

T

G

G

A

V

F

F

N

L

M

C

L

T

Q

K

A

A

V

V

G

S

M

K

D

L

M

M

I

L

A

-

R

K

A

Q

K

K

S

S

G

G

E

R

S

-

D

-

P

-

G

-

A

-

I

I

V

I

N

N

I

I

S

T

S

S

D

V

A

A

G

G

R

M

K

M

G

G

T

N

I
x
P

G

G

Q

Q

I

A

N

N

Y

Y

G

S

A

A

K
|
K

S

A

G

G

V

V

L

I

G

G

L

F

T

T

M

K

S

T

A

V

A

A

R

K

E

E

W

L

G

A

R

S

Y

R

G

G

I

V

R

T

T

V

N

N

S

A

V

V

A

A

Y

P

G

G

I
x
F

V

I

E

A

T

T

D

D

M

M

T

T

E

E

T

N

V

L

R
x
N

S

A

E

E

K

P

F

I

R

-

D

-

R

-

Y

-

L

L

A

Q

N

F

I

I

P

P

L

L

G

A

R

R

F

Y

L

G

T

Q

K

P

E

E

A

V

A

A

N

G

S

T

I

I

A

R

F

F

L

L

L

A

S

T

-

D

P

P

A

A

F

Y

I

I

T

T

G

G

Q

Q

H

T

L

F

S

N

V

V

N

D

G

G

A14 (= A13) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
P151 (≠ I153) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
K160 (= K162) mutation to A: Almost no activity on acetoacetyl-CoA.
F188 (≠ I190) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
N198 (≠ R200) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.

P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
39% identity, 99% coverage: 1:251/253 of query aligns to 1:243/244 of P0AEK2

query
sites
P0AEK2

M

M

N

N

L

F

S

E

G

G

R

K

T

I

I

A

L

L

I

V

T

T

G
|
G

A
|
A

A
x
S

Q
x
R

G

G

I

I

G

G

L

R

A

A

T

I

A

A

Q

E

L

T

C

L

A

A

L

R

D

G

A

A

N

K

V

V

I

I

L

G

L

T

D

A

R
x
T

S

S

E

E

E

N

Q

G

L

A

D

Q

A

A

A

-

L

I

A

S

S

D

F

Y

D

L

S

G

G

A

K

N

A

G

M

K

K

G

I

L

A

M

G

L

S
x
N

V
|
V

T

T

D

D

R

P

A

A

F

S

V

I

K

E

Q

S

A

V

V

L

A

E

E

K

A

I

A

R

A

A

R

E

F

F

G

G

G

E

I

V

H

D

G

I

L

L

V

V

N

N

N
|
N

A

A

G

G

I

I

T

T

R

R

T

D

A

N

M

L

I

L

D

M

K

R

M

M

T

K

A

D

D

E

W

W

Q

N

A

D

V

I

I

I

D

E

V

T

N

N

L

L

T

S

G

S

A

V

F

F

N

R

M

L

L

S

Q

K

A

A

V

V

G

M

M

R

D

A

M

M

I

M

A

K

R

K

A

R

K

H

S

-

G

-

E

-

S

-

D

-

P

-

G

G

A

R

I

I

V

I

N

T

I

I

S

G

S

S

D

V

A

V

G

G

R

T

K

M

G

G

T

N

I

G

G

G

Q

Q

I

A

N

N

Y
|
Y

G
x
A

A
|
A

K
|
K

S

A

G

G

V

L

L

I

G

G

L

F

T

S

M

K

S

S

A

L

A

A

R

R

E

E

W

V

G

A

R

S

Y

R

G

G

I

I

R

T

T

V

N

N

S

V

V

V

A

A

Y

P

G

G

I

F

V
x
I

E

E

T

T

D

D

M

M

T

T

E

R

T

A

V

L

R

-

S

S

E

D

K

D

F

Q

R

R

D

A

R

G

Y

I

L

L

A

A

N

Q

I

V

P

P

L

A

G

G

R

R

F

L

L

G

T

G

K

A

E

Q

E

E

A

I

A

A

N

N

S

A

I

V

A

A

F

F

L

L

L

A

S

S

P

D

A

E

A

A

F

Y

I

I

T

T

G

G

Q
x
E

H

T

L

L

S

H

V

V

N

N

G

G

S

M

H

Y

I

M

GASR 12:15 (≠ GAAQ 12:15) binding NADP(+)
T37 (≠ R37) binding NADP(+)
NV 59:60 (≠ SV 60:61) binding NADP(+)
N86 (= N87) binding NADP(+)
Y151 (= Y158) mutation to F: Defect in the affinity for NADPH.
YAAAK 151:155 (≠ YGAAK 158:162) binding NADP(+)
A154 (= A161) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
K155 (= K162) mutation to A: Defect in the affinity for NADPH.
I184 (≠ V191) binding NADP(+)
E233 (≠ Q241) mutation to K: Decreases in the thermolability of the reductase; when associated with T-154.

7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
38% identity, 98% coverage: 1:248/253 of query aligns to 4:244/247 of 7tzpG

query
sites
7tzpG

M

M

N

K

L

L

S

A

G

S

R

K

T

T

I

A

L

I

I

V

T

T

G
|
G

A

A

Q
x
R

G
|
G

I
|
I

G

G

L

F

A

G

T

I

A

A

Q

Q

L

V

C

L

A

A

A

R

L

E

D

G

A

A

N

R

V

V

I

I

L

I

L

A

D
|
D

R
|
R

S

D

E

A

E

H

Q

G

L

E

D

A

A

A

L

A

A

S

S

L

F

R

D

E

S

S

G

G

-

A

K

Q

A

A

M

L

K

F

I

I

A

S

G
x
C

S

N

V
x
I

T

A

D

E

R

K

A

T

F

Q

V

V

K

E

Q

A

A

L

V

F

A

S

E

Q

A

A

A

E

R

A

F

F

G

G

G

P

I

V

H

D

G

I

L

L

V

V

N

N

N
|
N

A

A

G
|
G

I
|
I

T

N

R

R

T

D

A

A

M

M

I

L

D

H

K

K

M

L

T

T

A

E

D

A

D

D

W

W

Q

D

A

T

V

V

I

I

D

D

V
|
V

N

N

L

L

T

K

G

G

A

T

F

F

N

L

M

C

L

M

Q

Q

A

Q

V

A

G

A

M

I

D

R

M

M

I

R

A

E

R

R

A

G

K

-

S

-

G

-

E

-

S

-

D

-

P

A

G

G

A

R

I

I

V

I

N

N

I

I

S

A

S

S

D

-

A

A

G

S

R

W

K

L

G

G

T

N

I

V

G

G

Q

Q

I

T

N

N

Y
|
Y

G

S

A

A

A

S

K
|
K

S

A

G

G

V

V

L

V

G

G

L

M

T

T

M

K

S

T

A

A

A

C

R

R

E

E

W

L

G

A

R

K

Y

K

G

G

I

V

R

T

T

V

N

N

S

A

V

I

A

C

Y

P

G

G

I

F

V
x
I

E

D

T
|
T

D

D

M

M

T
|
T

E

R

T

G

V

V

R

-

S

P

E

E

K

N

F

V

R

W

D

Q

R

I

Y

M

L

V

A

S

N

K

I

I

P

P

L

A

G

G

R

Y

F

A

L

G

T

E

K

A

E

K

E

D

A

V

A

G

N

E

S

C

I

V

A

A

F

F

L

L

L

A

S

S

P

D

A

G

A

A

A

R

F

Y

I

I

T

N

G

G

Q

E

H

V

L

I

S

N

V

V

N

G

G

G

binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G12), R18 (≠ Q15), G19 (= G16), I20 (= I17), D39 (= D36), R40 (= R37), C63 (≠ G59), I65 (≠ V61), N91 (= N87), G93 (= G89), I94 (= I90), V114 (= V110), Y155 (= Y158), K159 (= K162), I188 (≠ V191), T190 (= T193), T193 (= T196)

4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
40% identity, 98% coverage: 1:248/253 of query aligns to 1:244/247 of 4jroC

query
sites
4jroC

M

M

N

T

L

L

S

Q

G

G

R

K

T

V

I

A

L

V

I

V

T

T

G
|
G

A

G

A
x
S

Q
x
R

G
|
G

I
|
I

G

G

L

R

A

D

T

I

A

A

Q

I

L

N

C

L

A

A

A

K

L

E

D

G

A

A

N

N

V

I

I

F

L

F

-
x
N

-
x
Y

-
x
N

-
x
G

-
x
S

L

P

D

E

R

A

S

A

E

E

Q

T

L

A

D

K

A

L

A

V

L

A

A

E

S

H

F

G

D

V

S

E

G

V

K

E

A

A

M

M

K

K

I

A

A
x
N

G
x
V

S

A

V

I

T

A

D

E

R

D

-

V

-

D

A

A

F

F

V

F

K

K

Q

Q

A

A

V

I

A

E

E

-

A

-

R

R

F

F

G

G

G

R

I

V

H

D

G

I

L

L

V

V

N

N

N
|
N

A
|
A

G

G

I
|
I

T

T

R

R

T

D

A

N

M

L

I

L

D

M

K

R

M

M

T

K

A

E

D

D

D

E

W

W

Q

D

A

D

V

V

I

I

D

N

V
x
I

N

N

L

L

T

K

G

G

A

T

F

F

N

L

M

C

L

T

Q

K

A

A

V

V

G

S

M

R

D

T

M

M

I

M

A

-

R

-

A

-

K

-

S

-

G

-

E

K

S

Q

D

R

P

A

G

G

A

K

I

I

V

I

N

N

I

M

S

A

S
|
S

D

V

A

V

G

G

R

L

K

I

G

G

T

N

I

A

G

G

Q
|
Q

I

A

N

N

Y
|
Y

G

V

A

A

A

S

K
|
K

S

A

G

G

V

V

L

I

G

G

L

L

T

T

M

K

S

T

A

T

A

A

R

R

E

E

W

L

G

A

R

P

Y

R

G

G

I

I

R

N

T

V

N

N

S

A

V

V

A

A

Y
x
P

G

G

I

F

V
x
I

E

T

T
|
T

D

D

M

M

T

T

E

D

T

K

V

L

R

-

S

D

E

E

K

K

F

T

R

K

D

E

R

A

Y

M

L

L

A

A

N

Q

I

I

P

P

L

L

G

G

R

A

F

Y

L

G

T

T

K

T

E

E

E

D

A

I

A

A

N

N

S

A

I

V

A

L

F

F

L

L

L

A

S

S

P

D

A

A

A

S

A

K

F

Y

I

I

T

T

G

G

Q

Q

H

T

L

L

S

S

V

V

N

D

G

G

active site: G16 (= G16), S142 (= S145), Q152 (= Q155), Y155 (= Y158), K159 (= K162)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (≠ Q15), G16 (= G16), I17 (= I17), N35 (vs. gap), Y36 (vs. gap), N37 (vs. gap), G38 (vs. gap), S39 (vs. gap), N63 (≠ A58), V64 (≠ G59), N90 (= N87), A91 (= A88), I93 (= I90), I113 (≠ V110), S142 (= S145), Y155 (= Y158), K159 (= K162), P185 (≠ Y188), I188 (≠ V191), T190 (= T193)

6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
39% identity, 99% coverage: 1:251/253 of query aligns to 1:243/244 of 6t77A

query
sites
6t77A

M

M

N

S

L

F

S

E

G

G

R

K

T

I

I

A

L

L

I

V

T

T

G
|
G

A

A

A
x
S

Q
x
R

G
|
G

I

I

G

G

L

R

A

A

T

I

A

A

Q

E

L

T

C

L

A

V

A

A

L

R

D

G

A

A

N

K

V

V

I

I

L

G

L

T

D

A

R
x
T

S

S

E

E

E

S

Q

G

L

A

D

Q

A

A

A

-

L

I

A

S

S

D

F

Y

D

L

S

G

G

A

K

N

A

G

M

K

K

G

I

L

A

M

G
x
L

S
x
N

V
|
V

T

T

D

D

R

P

A

A

F

S

V

I

K

E

Q

S

A

V

V

L

A

E

E

N

A

V

A

R

A

A

R

E

F

F

G

G

G

E

I

V

H

D

G

I

L

L

V

V

N

N

A
|
A

G
|
G

I
|
I

T

T

R

R

T

D

A

N

M

L

I

L

D

M

K

R

M

M

T

K

A

D

D

D

D

E

W

W

Q

N

A

D

V

I

I

I

D

E

V

T

N

N

L

L

T

S

G

S

A

V

F

F

N

R

M

L

L

S

Q

K

A

A

V

V

G

M

M

R

D

A

M

M

I

M

A

K

R

K

A

R

K

H

S

-

G

-

E

-

S

-

D

-

P

-

G

G

A

R

I

I

V

I

N

T

I

I

S

G

S
|
S

D

V

A

V

G

G

R

T

K

M

G

G

T

N

I

A

G

G

Q

Q

I

A

N

N

Y
|
Y

G

A

A

A

K

K

S

A

G

G

V

L

L

I

G

G

L

F

T

S

M

K

S

S

A

L

A

A

R

R

E

E

W

V

G

A

R

S

Y

R

G

G

I

I

R

T

T

V

N

N

S

V

V

V

A

A

Y

P

G

G

I

F

V

I

E

E

T

T

D

D

M

M

T

T

E

R

T

A

V

L

R

T

S

D

E

E

K

Q

F

-

R

R

D

A

R

G

Y

T

L

L

A

A

N

A

I

V

P

P

L

A

G

G

R

R

F

L

L

G

T

T

K

P

E

N

E

E

A

I

A

A

N

S

S

A

I

V

A

A

F

F

L

L

L

A

S

S

P

D

A

E

A

A

A

S

F

Y

I

I

T

T

G

G

Q

E

H

T

L

L

S

H

V

V

N

N

G

G

S

M

H

Y

I

M

active site: G16 (= G16), S138 (= S145), Y151 (= Y158)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (≠ Q15), T37 (≠ R37), L58 (≠ G59), N59 (≠ S60), V60 (= V61), A87 (= A88), G88 (= G89), I89 (= I90)

1q7bA The structure of betaketoacyl-[acp] reductase from e. Coli in complex with NADP+ (see paper)
39% identity, 99% coverage: 2:251/253 of query aligns to 1:242/243 of 1q7bA

query
sites
1q7bA

N

N

L

F

S

E

G

G

R

K

T

I

I

A

L

L

I

V

T

T

G
|
G

A

A

A
x
S

Q
x
R

G
|
G

I

I

G

G

L

R

A

A

T

I

A

A

Q

E

L

T

C

L

A

A

L

R

D

G

A

A

N

K

V

V

I

I

L

G

L

T

D

A

R
x
T

S

S

E

E

E

N

Q

G

L

A

D

Q

A

A

A

-

L

I

A

S

S

D

F

Y

D

L

S

G

G

A

K

N

A

G

M

K

K

G

I

L

A

M

G

L

S
x
N

V
|
V

T

T

D

D

R

P

A

A

F

S

V

I

K

E

Q

S

A

V

V

L

A

E

E

K

A

I

A

R

A

A

R

E

F

F

G

G

G

E

I

V

H

D

G

I

L

L

V

V

N

N

N
|
N

A
|
A

G
|
G

I
|
I

T

T

R

R

T

D

A

N

M

L

I

L

D

M

K

R

M

M

T

K

A

D

D

E

D
x
E

W

W

Q

N

A

D

V

I

I

I

D

E

V

T

N

N

L

L

T

S

G

S

A

V

F

F

N

R

M

L

L

S

Q

K

A

A

V

V

G

M

M

R

D

A

M

M

I

M

A

K

R

K

A

R

K

H

S

-

G

-

E

-

S

-

D

-

P

-

G

G

A

R

I

I

V

I

N

T

I

I

S

G

S
|
S

D

V

A

V

G

G

R

T

K

M

G

G

T

N

I

G

G

G

Q
|
Q

I

A

N

N

Y
|
Y

G

A

A

A

K
|
K

S

A

G

G

V

L

L

I

G

G

L

F

T

S

M

K

S

S

A

L

A

A

R

R

E

E

W

V

G

A

R

S

Y

R

G

G

I

I

R

T

T

V

N

N

S

V

V

V

A

A

Y
x
P

G
|
G

I

F

V
x
I

E

E

T

T

D

D

M

M

T

T

E

R

T

A

V

L

R

-

S

S

E

D

K

D

F

Q

R

R

D

A

R

G

Y

I

L

L

A

A

N

Q

I

V

P

P

L

A

G

G

R

R

F

L

L

G

T

G

K

A

E

Q

E

E

A

I

A

A

N

N

S

A

I

V

A

A

F

F

L

L

L

A

S

S

P

D

A

E

A

A

F

Y

I

I

T

T

G

G

Q
x
E

H
x
T

L

L

S

H

V

V

N

N

G

G

S

M

H

Y

I

M

active site: G15 (= G16), E101 (≠ D103), S137 (= S145), Q147 (= Q155), Y150 (= Y158), K154 (= K162)
binding calcium ion: E232 (≠ Q241), T233 (≠ H242)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G12), S13 (≠ A14), R14 (≠ Q15), T36 (≠ R37), N58 (≠ S60), V59 (= V61), N85 (= N87), A86 (= A88), G87 (= G89), I88 (= I90), S137 (= S145), Y150 (= Y158), K154 (= K162), P180 (≠ Y188), G181 (= G189), I183 (≠ V191)

1q7cA The structure of betaketoacyl-[acp] reductase y151f mutant in complex with NADPH fragment (see paper)
39% identity, 99% coverage: 2:251/253 of query aligns to 1:242/243 of 1q7cA

query
sites
1q7cA

N

N

L

F

S

E

G

G

R

K

T

I

I

A

L

L

I

V

T

T

G
|
G

A

A

A
x
S

Q
x
R

G
|
G

I

I

G

G

L

R

A

A

T

I

A

A

Q

E

L

T

C

L

A

A

L

R

D

G

A

A

N

K

V

V

I

I

L

G

L

T

D
x
A

R
x
T

S

S

E

E

E

N

Q

G

L

A

D

Q

A

A

A

-

L

I

A

S

S

D

F

Y

D

L

S

G

G

A

K

N

A

G

M

K

K

G

I

L

A

M

G
x
L

S
x
N

V
|
V

T

T

D

D

R

P

A

A

F

S

V

I

K

E

Q

S

A

V

V

L

A

E

E

K

A

I

A

R

A

A

R

E

F

F

G

G

G

E

I

V

H

D

G

I

L

L

V

V

N

N

A

A

G
|
G

I
|
I

T

T

R

R

T

D

A

N

M

L

I

L

D

M

K

R

M

M

T

K

A

D

D

E

W

W

Q

N

A

D

V

I

I

I

D

E

V

T

N

N

L

L

T

S

G

S

A

V

F

F

N

R

M

L

L

S

Q

K

A

A

V

V

G

M

M

R

D

A

M

M

I

M

A

K

R

K

A

R

K

H

S

-

G

-

E

-

S

-

D

-

P

-

G

G

A

R

I

I

V

I

N

T

I

I

S

G

S
|
S

D

V

A

V

G

G

R

T

K

M

G

G

T

N

I

G

G

G

Q
|
Q

I

A

N

N

Y
x
F

G

A

A

A

K
|
K

S

A

G

G

V

L

L

I

G

G

L

F

T

S

M

K

S

S

A

L

A

A

R

R

E

E

W

V

G

A

R

S

Y

R

G

G

I

I

R

T

T

V

N

N

S

V

V

V

A

A

Y

P

G

G

I

F

V

I

E

E

T

T

D

D

M

M

T

T

E

R

T

A

V

L

R

-

S

S

E

D

K

D

F

Q

R

R

D

A

R

G

Y

I

L

L

A

A

N

Q

I

V

P

P

L

A

G

G

R

R

F

L

L

G

T

G

K

A

E

Q

E

E

A

I

A

A

N

N

S

A

I

V

A

A

F

F

L

L

L

A

S

S

P

D

A

E

A

A

F

Y

I

I

T

T

G

G

Q

E

H

T

L

L

S

H

V

V

N

N

G

G

S

M

H

Y

I

M

active site: G15 (= G16), S137 (= S145), Q147 (= Q155), F150 (≠ Y158), K154 (= K162)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G12), S13 (≠ A14), R14 (≠ Q15), A35 (≠ D36), T36 (≠ R37), L57 (≠ G59), N58 (≠ S60), V59 (= V61), G87 (= G89), I88 (= I90)

P0A2C9 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
39% identity, 99% coverage: 1:251/253 of query aligns to 1:243/244 of P0A2C9

query
sites
P0A2C9

M

M

N

S

L

F

S

E

G

G

R

K

T

I

I

A

L

L

I

V

T

T

G

G

A

A

A

S

Q

R

G

G

I

I

G

G

L

R

A

A

T

I

A

A

Q

E

L

T

C

L

A

V

A

A

L

R

D

G

A

A

N

K

V

V

I

I

L

G

L

T

D

A

R

T

S

S

E

E

-

N

-

G

-

A

E

K

Q

N

L

I

D

S

A

D

A

Y

L

L

A

G

S

A

F

-

D

-

S

N

G

G

K

K

A

G

M

L

K

M

I

L

A

-

G

-

S

N

V

V

T

T

D

D

R

P

A

A

F

S

V

I

K

E

Q

S

A

V

V

L

A

E

E

N

A

I

A

R

A

A

R

E

F

F

G

G

G

E

I

V

H

D

G

I

L

L

V

V

N

N

A

A

G

G

I

I

T

T

R

R

T

D

A

N

M

L

I

L

D

M

K

R

M

M

T

K

A

D

D

D

D

E

W

W

Q

N

A

D

V

I

I

I

D

E

V

T

N

N

L

L

T

S

G

S

A

V

F

F

N

R

M

L

L

S

Q

K

A

A

V

V

G

M

M

R

D

A

M
|
M

I

M

A

K

R

K

A

-

K

-

S

-

G

-

E

-

S

-

D

R

P

C

G

G

A

R

I

I

V

I

N

T

I

I

S

G

S

S

D

V

A

V

G

G

R

T

K

M

G

G

T

N

I

A

G

G

Q

Q

I

A

N

N

Y

Y

G

A

A

A

K

K

S

A

G

G

V

L

L

I

G

G

L

F

T

S

M

K

S

S

A

L

A

A

R

R

E

E

W

V

G

A

R

S

Y

R

G

G

I

I

R

T

T

V

N

N

S

V

V

V

A

A

Y

P

G

G

I

F

V

I

E

E

T

T

D

D

M

M

T

T

E

R

T

A

V

L

R

-

S

S

E

D

K

D

F

Q

R

R

D

A

R

G

Y

I

L

L

A

A

N

Q

I

V

P

P

L

A

G

G

R

R

F

L

L

G

T

G

K

A

E

Q

E

E

A

I

A

A

N

S

S

A

I

V

A

A

F

F

L

L

L
x
A

S
|
S

P

D

A

E

A

A

A

S

F

Y

I

I

T

T

G

G

Q

E

H

T

L

L

S

H

V

V

N

N

G

G

S

M

H

Y

I

M

M125 (= M126) mutation to I: Loss of the temperature-sensitive phenotype; when associated with T-223.
A223 (≠ L231) mutation to T: Loss of the temperature-sensitive phenotype; when associated with I-125.
S224 (= S232) mutation to F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.

4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
39% identity, 98% coverage: 3:251/253 of query aligns to 3:239/239 of 4nbtA

query
sites
4nbtA

L

L

S

E

G

G

R

K

T

V

I

A

L

V

I

I

T

T

G
|
G

A

G

A

A

Q
x
K

G
|
G

I
x
L

G

G

L

Q

A

A

T

I

A

A

Q

L

L

A

C

Y

A

A

A

E

L

E

D

G

A

A

N

K

V

V

I

I

L

A

L

G

D
|
D

R
x
L

S

G

E

D

E

-

Q

-

L

-

D

-

A

-

L

-

A

L

S

T

F

Y

D

S

S

H

G

P

K

N

A

V

M

E

K

G

I

M

A

Y

G
x
L

S
x
N

V
|
V

T

T

D

D

R

V

A

T

F

G

V

V

K

E

Q

K

A

F

V

Y

A

Q

E

S

A

V

A

I

A

D

R

K

F

Y

G

G

G

K

I

I

H

D

G

I

L

L

V

V

N

N

N
|
N

A
|
A

G
|
G

I

I

T

T

R

K

T

D

A

A

M

M

I

T

D

R

K

K

M

M

T

T

A

E

D

A

D

Q

W

W

Q

D

A

A

V

V

I

I

D

D

V

V

N

N

L

L

T

K

G

G

A

V

F

F

N

N

M

L

L

T

Q

R

A

L

V

V

G

G

M

P

D

Q

M

M

I

Q

A

T

R

N

A

G

K

Y

S

-

G

-

E

-

S

-

D

-

P

-

G

G

A

S

I

I

V

I

N

N

I
|
I

S

S

S
|
S

D

V

A

V

G

G

R

V

K

F

G

G

T

N

I

I

G

G

Q

Q

I

A

N

N

Y
|
Y

G

A

A

A

A

T

K
|
K

S

A

G

G

V

V

L

I

G

G

L

L

T

T

M

M

S

T

A

W

A

A

R

K

E

E

W

F

G

A

R

L

Y

K

G

G

-

A

-

N

I

V

R

R

T

V

N

N

S

A

V

I

A

A

Y
x
P

G
|
G

I

Y

V
x
I

E

M

T
|
T

D

D

M

I

T

L

E

K

T

T

V

V

R

-

S

P

E

Q

K

D

F

L

R

L

D

D

R

K

Y

F

L

A

A

A

N

L

I

T

P

M

L

L

G

N

R

R

F

L

L

G

T

Q

K

P

E

E

A

I

A

A

N

K

S

V

I

A

A

L

F

F

L

L

L

A

S

S

P

D

A

D

A

A

A

S

F

Y

I

V

T

T

G

G

Q

Q

H

T

L

I

S

N

V

V

N

N

G

G

S

M

H

R

I

L

active site: G16 (= G16), S132 (= S145), Y145 (= Y158), K149 (= K162)
binding nicotinamide-adenine-dinucleotide: G12 (= G12), K15 (≠ Q15), G16 (= G16), L17 (≠ I17), D36 (= D36), L37 (≠ R37), L52 (≠ G59), N53 (≠ S60), V54 (= V61), N80 (= N87), A81 (= A88), G82 (= G89), I130 (= I143), S132 (= S145), Y145 (= Y158), K149 (= K162), P177 (≠ Y188), G178 (= G189), I180 (≠ V191), T182 (= T193)

7emgB Carbonyl reductase variant 4 (r123c/l209p/f183y/v61k) from serratia marcescens complexed with NADP+ (see paper)
38% identity, 99% coverage: 2:251/253 of query aligns to 1:242/243 of 7emgB

query
sites
7emgB

N

S

L

F

S

E

G

G

R

K

T

I

I

V

L

L

I

V

T

T

G
|
G

A

A

A
x
S

Q
x
R

G

G

I

I

G

G

L

R

A

A

T

I

A

A

Q

E

L

T

C

F

A

V

A

A

L

R

D

G

A

A

N

K

V

V

I

I

L

G

L

T

D

A

R
x
T

S

S

E

E

E

S

Q

G

L

A

D

E

A

A

A

I

L

S

A

G

S

Y

F

L

D

G

S

A

-

N

G

G

K

K

A

G

M

F

K

M

I

L

A

-

G

-

S
x
N

V
|
V

T

K

D

D

R

A

A

Q

F

S

V

I

K

D

Q

S

A

V

V

L

A

A

E

S

A

I

A

R

A

A

R

E

F

F

G

G

G

E

I

I

H

D

G

I

L

L

V

V

N

N

A

A

G

G

I
|
I

T

T

R

R

T

D

A

N

M

L

I

L

D

M

K

R

M

M

T

K

A

D

D

D

D

E

W

W

Q

E

A

D

V

I

I

L

D

D

V

T

N

N

L

L

T

T

G

S

A

V

F

F

N

R

M

L

L

S

Q

K

A

A

V

V

G

M

M

C

D

A

M

M

I

M

A

K

R

K

A

-

K

-

S

-

G

-

E

-

S

-

D

R

P

F

G

G

A

R

I

I

V

I

N

T

I

I

S

G

S

S

D

V

A

V

G

G

R

T

K

M

G

G

T

N

I

A

G

G

Q

Q

I

A

N

N

Y

Y

G

A

A

A

K

K

S

A

G

G

V

L

L

I

G

G

L

F

T

S

M

K

S

S

A

L

A

A

R

R

E

E

W

V

G

A

R

S

Y

R

G

G

I

I

R

T

T

V

N

N

S

V

V

V

A

A

Y

P

G

G

I

Y

V

I

E

E

T

T

D

D

M

M

T

T

E

R

T

A

V

L

R

T

S

D

E

D

K

Q

F

-

R

R

D

A

R

G

Y

I

L

L

A

S

N

S

I

V

P

P

L

A

G

N

R

R

F

P

L

G

T

D

K

A

E

K

E

E

A

I

A

A

N

S

S

A

I

V

A

A

F

F

L

L

L

A

S

S

P

D

A

E

A

A

A

G

F

Y

I

I

T

T

G

G

Q

E

H

T

L

L

S

H

V

V

N

N

G

G

S

M

H

Y

I

M

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G12), S13 (≠ A14), R14 (≠ Q15), T36 (≠ R37), N58 (≠ S60), V59 (= V61), I88 (= I90)

5vmlA Crystal structure of acetoacetyl-coa reductase from burkholderia pseudomallei 1710b with bound NADP
42% identity, 76% coverage: 59:251/253 of query aligns to 59:244/245 of 5vmlA

query
sites
5vmlA

G
|
G

S
x
N

V
|
V

T

G

D

D

R

W

A

D

F

S

V

T

K

K

Q

Q

A

A

V

F

A

D

E

K

A

V

A

K

A

A

R

E

F

V

G

G

G

E

I

I

H

D

G

V

L

L

V

V

N

N

N
|
N

A

A

G
|
G

I
|
I

T

T

R

R

T

D

A

V

M

V

I

F

D

R

K

K

M

M

T

T

A

R

D

E

D

D

W

W

Q

Q

A

A

V

V

I

I

D

D

V

T

N
|
N

L

L

T

T

G

S

A

L

F

F

N

N

M

V

L

T

Q

K

A

Q

V

V

G

I

M

D

D

G

M

M

I

V

A

E

R

R

A

G

K

W

S

-

G

-

E

-

S

-

D

-

P

-

G

G

A

R

I

I

V

I

N

N

I

I

S

S

S
|
S

D

V

A

N

G

G

R

Q

K

K

G

G

T

Q

I

F

G

G

Q

Q

I

T

N

N

Y
|
Y

G

S

A

T

A

A

K
|
K

S

A

G

G

V

I

L

H

G

G

L

F

T

T

M

M

S

S

A

L

A

A

R

Q

E

E

W

V

G

A

R

T

Y

K

G

G

I

V

R

T

T

V

N

N

S

T

V

V

A

S

Y
x
P

G
|
G

I

Y

V
x
I

E

G

T

T

D

D

M

M

T

V

E

K

T

A

V

I

R

R

S

P

E

D

K

V

F

L

R

-

D

E

R

K

Y

I

L

V

A

A

N

T

I

I

P

P

L

V

G

R

R

R

F

L

L

G

T

S

K

P

E

D

E

E

A

I

A

G

N

S

S

I

I

V

A

A

F

W

L

L

L

A

S

S

P

E

A

E

A

S

A

G

F

F

I

S

T

T

G

G

Q

A

H

D

L

F

S

S

V

L

N

N

G

G

S

L

H

H

I

M

active site: N111 (= N111), S139 (= S145), Y152 (= Y158), K156 (= K162)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G59 (= G59), N60 (≠ S60), V61 (= V61), N87 (= N87), G89 (= G89), I90 (= I90), S139 (= S145), Y152 (= Y158), K156 (= K162), P182 (≠ Y188), G183 (= G189), I185 (≠ V191)

Sites not aligning to the query:

active site: 13
binding nadp nicotinamide-adenine-dinucleotide phosphate: 9, 12, 13, 14, 33, 34, 39

1edoA The x-ray structure of beta-keto acyl carrier protein reductase from brassica napus complexed with NADP+ (see paper)
38% identity, 96% coverage: 8:251/253 of query aligns to 4:244/244 of 1edoA

query
sites
1edoA

I

V

L

V

I

V

T

T

G
|
G

A

A

A
x
S

Q
x
R

G
|
G

I
|
I

G

G

L

K

A

A

T

I

A

A

Q

L

L

S

-

L

-

G

-

K

-

A

-

G

C

C

A

K

A

V

L

L

D

-

A

V

N
|
N

V
x
Y

I
x
A

L
x
R

L
x
S

D

A

R

K

S

A

E

A

E

E

Q

E

L

V

D

S

A

K

A

Q

L

I

A

E

S

A

F

Y

D

-

S

G

G

G

K

Q

A

A

M

I

K

T

I

F

A

G

G

G

S
x
D

V
|
V

T

S

D

K

R

E

A

A

F

D

V

V

K

E

Q

A

A

M

V

M

A

K

E

T

A

A

A

I

A

D

R

A

F

W

G

G

G

T

I

I

H

D

G

V

L

V

V

V

N

N

N
|
N

A
|
A

G

G

I

I

T

T

R

R

T

D

A

T

M

L

I

L

D

I

K

R

M

M

T

K

A

K

D

S

D

Q

W

W

Q

D

A

E

V

V

I

I

D

D

V

L

N

N

L

L

T

T

G

G

A

V

F

F

N

L

M

C

L

T

Q

Q

A

A

V

A

G

T

M

K

D

I

M

M

I

M

A

K

R

K

A

R

K

K

S

-

G

-

E

-

S

-

D

-

P

-

G

G

A

R

I

I

V

I

N

N

I

I

S

A

S
|
S

D

V

A

V

G

G

R

L

K

I

G

G

T

N

I

I

G

G

Q

Q

I

A

N

N

Y
|
Y

G

A

A

A

K
|
K

S

A

G

G

V

V

L

I

G

G

L

F

T

S

M

K

S

T

A

A

R

R

E

E

W

G

G

A

R

S

Y

R

G

N

I

I

R

N

T

V

N

N

S

V

V

V

A

C

Y
x
P

G
|
G

I

F

V
x
I

E

A

T
x
S

D

D

M
|
M

T

T

E

A

T

K

V

L

R

-

S

G

E

E

K

D

F

M

R

E

D

K

R

K

Y

I

L

L

A

G

N

T

I

I

P

P

L

L

G

G

R

R

F

T

L

G

T

Q

K

P

E

E

E

N

A

V

A

A

N

G

S

L

I

V

A

E

F

F

L

L

-

A

L

L

S

S

P

P

A

A

A

S

F

Y

I

I

T

T

G

G

Q

Q

H

A

L

F

S

T

V

I

N

D

G

G

S

I

H

A

I

I

active site: G12 (= G16), S138 (= S145), Y151 (= Y158), K155 (= K162)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), S10 (≠ A14), R11 (≠ Q15), I13 (= I17), N31 (= N31), Y32 (≠ V32), A33 (≠ I33), R34 (≠ L34), S35 (≠ L35), D59 (≠ S60), V60 (= V61), N86 (= N87), A87 (= A88), S138 (= S145), Y151 (= Y158), K155 (= K162), P181 (≠ Y188), G182 (= G189), I184 (≠ V191), S186 (≠ T193), M188 (= M195)

5u9pB Crystal structure of a gluconate 5-dehydrogenase from burkholderia cenocepacia j2315 in complex with NADP and tartrate
39% identity, 98% coverage: 1:248/253 of query aligns to 12:256/261 of 5u9pB

query
sites
5u9pB

M

F

N

R

L

L

S

D

G

G

R

R

T

R

I

A

L

L

I

I

T

T

G
|
G

A

S

A

G

Q
x
R

G
|
G

I
|
I

G

G

L

L

A

T

T

L

A

A

Q

R

L

G

C

L

A

A

A

E

L

A

D

G

A

A

N

A

V

I

I

V

L

I

L

N

D

D

R
|
R

S

N

E

E

Q

K

L

A

D

A

A

T

A

L

L

A

A

R

S

R

F

F

-

R

D

D

S

E

G

G

K

F

A

A

M

A

K

D

I

H

A

A

-

V

G

F

S
x
D

V
|
V

T

A

D

E

R

H

A

A

F

Q

V

V

K

R

Q

A

A

A

V

I

A

D

E

E

A

F

A

E

A

A

R

R

F

V

G

G

G

A

I

I

H

D

G

I

L

L

V

V

N

N

N
|
N

A
|
A

G

G

I

I

T

Q

R

R

T

R

A

A

M

P

I

L

D

D

K

A

M

F

T

E

A

P

D

D

W

W

Q

H

A

A

V

L

I

M

D

R

V

V

N

N

L

L

T

D

G

G

A

V

F

F

N

N

M

V

L

A

Q

Q

A

A

V

V

G

A

M

R

D

H

M

M

I

I

A

A

R

R

A

G

K

H

S

-

G

-

E

-

S

-

D

-

P

-

G

G

A

K

I

I

V

I

N

N

I
|
I

S

C

S
|
S

D

V

A

Q

G

S

R

E

K

L

G

A

T

R

I

P

G

T

Q

I

I

A

N

P

Y
|
Y

G

A

A

A

A

T

K
|
K

S

G

G

A

V

V

L

R

G

M

L

L

T

T

M

K

S

G

A

M

A

C

R

A

E

D

W

W

G

A

R

R

Y

Y

G

G

I

I

R

Q

T

A

N

N

S

G

V

L

A

A

Y
x
P

G

G

I

Y

V
x
F

E

E

T
|
T

D

E

M
x
L

T
x
N

E

R

T

A

-

L

V

V

R

D

S

D

E

A

K

A

F

F

R

S

D

D

R

W

Y

L

L

C

A

K

N

R

I

T

P

P

L

A

G

G

R

R

F

W

L

G

T

Q

K

V

E

D

E

E

A

L

A

C

N

G

S

A

I

A

A

I

F

F

L

L

L

A

S

S

P

A

A

A

A

S

A

D

F

F

I

V

T

N

G

G

Q

Q

H

T

L

L

S

F

V

V

N

D

G

G

active site: G27 (= G16), S152 (= S145), Y165 (= Y158), K169 (= K162)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G23 (= G12), R26 (≠ Q15), G27 (= G16), I28 (= I17), R48 (= R37), D73 (≠ S60), V74 (= V61), N100 (= N87), A101 (= A88), I150 (= I143), Y165 (= Y158), K169 (= K162), P195 (≠ Y188), F198 (≠ V191), T200 (= T193), L202 (≠ M195), N203 (≠ T196)

3tzcA Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (fabg)(y155f) from vibrio cholerae (see paper)
38% identity, 99% coverage: 1:250/253 of query aligns to 4:224/224 of 3tzcA

query
sites
3tzcA

M

M

N

N

L

L

S

E

G

G

R

K

T

V

I

A

L

L

I

V

T

T

G
|
G

A

A

A
x
S

Q
x
R

G

G

I

I

G

G

L

K

A

A

T

I

A

A

Q

E

L

L

C

L

A

A

A

E

L

R

D

G

A

A

N

K

V

V

I

I

L

G

L

T

D
x
A

R
x
T

S

S

E

E

E

S

Q

G

L

A

D

Q

A

A

A

-

L

I

A

S

S

D

F

Y

-

L

-

G

D

D

S

N

G

G

K

K

A

G

M

M

K

A

I

L

A

-

G

-

S
x
N

V
|
V

T

T

D

N

R

P

A

E

F

S

V

I

K

E

Q

A

A

V

V

L

A

K

E

A

A

I

A

T

A

D

R

E

F

F

G

G

I

V

H

D

G

I

L

L

V

V

N

N

A

A

G
|
G

I
|
I

T

T

R

R

T

M

A

-

M

-

I

-

D

-

K

R

M

M

T

K

A

E

D

E

W

W

Q

S

A

D

V

I

I

M

D

E

V
x
T

N

N

L

L

T

T

G

S

A

I

F

F

N

R

M

L

L

S

Q

K

A

A

V

V

G

L

M

R

D

G

M

M

I

M

A

K

R

K

A

R

K

Q

S

-

G

-

E

-

S

-

D

-

P

-

G

G

A

R

I

I

V

I

N

N

I

V

S

G

S

S

D

V

A

V

G

-

R

-

K

-

G

-

T

-

I

-

G

-

Q

-

I

-

N

-

Y

-

G

-

A

A

K

K

S

A

G

G

V

V

L

I

G

G

L

F

T

T

M

K

S

S

A

M

A

A

R

R

E

E

W

V

G

A

R

S

Y

R

G

G

I

V

R

T

T

V

N

N

S

T

V

V

A

A

Y

P

G

G

I

F

V

I

E

E

T

T

D

D

M

M

T

D

E

E

T

Q

V

-

R

-

S

-

E

-

K

-

F

-

R

R

D

T

R

A

Y

T

L

L

A

A

N

Q

I

V

P

P

L

A

G

G

R

R

F

L

L

G

T

D

K

P

E

R

E

E

A

I

A

A

N

S

S

A

I

V

A

A

F

F

L

L

L

A

S

S

P

P

A

E

A

A

F

Y

I

I

T

T

G

G

Q

E

H

T

L

L

S

H

V

V

N

N

G

G

S

M

H

Y

binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), S17 (≠ A14), R18 (≠ Q15), A39 (≠ D36), T40 (≠ R37), N62 (≠ S60), V63 (= V61), G91 (= G89), I92 (= I90), T108 (≠ V110)

Query Sequence

>Ga0059261_2801 FitnessBrowser__Korea:Ga0059261_2801
MNLSGRTILITGAAQGIGLATAQLCAALDANVILLDRSEEQLDAALASFDSGKAMKIAGS
VTDRAFVKQAVAEAAARFGGIHGLVNNAGITRTAMIDKMTADDWQAVIDVNLTGAFNMLQ
AVGMDMIARAKSGESDPGAIVNISSDAGRKGTIGQINYGAAKSGVLGLTMSAAREWGRYG
IRTNSVAYGIVETDMTETVRSEKFRDRYLANIPLGRFLTKEEAANSIAFLLSPAAAFITG
QHLSVNGGSHITA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory