SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ga0059261_2836 Ga0059261_2836 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3w6zA Crystal structure of NADP bound l-serine 3-dehydrogenase (k170m) from hyperthermophilic archaeon pyrobaculum calidifontis (see paper)
30% identity, 91% coverage: 1:263/290 of query aligns to 14:288/296 of 3w6zA

query
sites
3w6zA

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binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G7), L21 (= L8), G22 (= G9), I23 (≠ D10), M24 (≠ Q11), N43 (≠ A30), R44 (= R31), T45 (≠ R32), K48 (≠ A35), V77 (= V64), S78 (≠ T65), D82 (= D69), Q85 (≠ E72), V133 (= V119), F244 (vs. gap), K245 (≠ R225), H248 (= H228), K251 (= K235)

3ws7A The 1.18 a resolution structure of l-serine 3-dehydrogenase complexed with NADP+ and sulfate ion from the hyperthermophilic archaeon pyrobaculum calidifontis (see paper)
30% identity, 91% coverage: 1:263/290 of query aligns to 14:285/293 of 3ws7A

query
sites
3ws7A

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binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G7), L21 (= L8), G22 (= G9), I23 (≠ D10), M24 (≠ Q11), N43 (≠ A30), R44 (= R31), T45 (≠ R32), K48 (≠ A35), M76 (≠ C63), V77 (= V64), S78 (≠ T65), D82 (= D69), Q85 (≠ E72), V133 (= V119), F241 (vs. gap), K242 (≠ R225), H245 (= H228), K248 (= K235)
binding sulfate ion: T134 (≠ S120), G135 (= G121), K183 (= K169)

P29266 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Rattus norvegicus (Rat) (see paper)
31% identity, 83% coverage: 3:243/290 of query aligns to 41:291/335 of P29266

query
sites
P29266

V

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D68 (≠ A30) mutation to R: Decrease of activity with NAD, increase of activity with NADP.
K208 (= K169) mutation K->A,H,N,R: Complete loss of activity.
N212 (= N173) mutation to Q: Decrease in activity.

2i9pB Crystal structure of human hydroxyisobutyrate dehydrogenase complexed with NAD+
31% identity, 84% coverage: 1:243/290 of query aligns to 1:253/296 of 2i9pB

query
sites
2i9pB

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binding nicotinamide-adenine-dinucleotide: G9 (= G9), N10 (≠ D10), M11 (≠ Q11), Y29 (≠ W29), D30 (≠ A30), V31 (≠ R31), M63 (≠ L62), L64 (≠ C63), P65 (≠ V64), T95 (= T94), V120 (= V119), G122 (= G121), F238 (vs. gap), K245 (= K235)

P31937 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Homo sapiens (Human) (see paper)
31% identity, 83% coverage: 3:243/290 of query aligns to 42:292/336 of P31937

query
sites
P31937

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N

D

P

P

K

A

T

V

C

S

V

K

E

E

L

L

A

A

R

K

M

E

V

V

S

E

A

K

R

M

G

G

A

A

T

V

L

F

L

M

D

D

M

A

P

P

V

V

S

S

G

G

S

G

G

V

H

G

A

A

L

R

A

S

R

G

K

N

L

L

L

T

V

F

M

M

T

V

G

G

D

V

A

E

G

D

A

E

I

F

A

A

R

A

T

A

M

Q

P

E

V

L

L

L

E

G

S

C

Y

M

A

G

G

S

T

N

I

V

R

Y

M

C

G

G

D

A

V

V

G

G

A

T

A

G

M

Q

N

A

A

A

K

K

L

I

V

C

N

N

-

M

L

L

M

L

A

A

V

I

V

S

N

M

I

I

G

G

Q

T

A

A

F

-

H

E

A

A

L

M

A

N

L

L

G

G

R

I

K

R

V

L

G

G

V

L

E

D

P

P

A

K

A

L

L

L

R

A

Q

K

A

I

L

L

M

N

V

M

G

S

T

S

G

G

R

R

S

C

F

W

A

S

I

S

D

D

L

T

I

Y

A

N

R

P

L

V

H

P

-

G

-

V

-

M

-

D

-

G

V

V

P

P

K

S

R

A

A

N

H

N

H

Y

V

Q

R

G

G

G

-

F

-

G

-

T

V

L

L

M

V

A

K
|
K

D

D

V

L

D

G

L

L

A

A

I

Q

E

D

A

S

LP 103:104 (≠ CV 63:64) binding
N108 (≠ A68) binding
T134 (= T94) binding
K284 (= K235) binding

Sites not aligning to the query:

1:36 modified: transit peptide, Mitochondrion
40:68 binding

5u5gA Psf3 in complex with NADP+ and 2-opp (see paper)
29% identity, 91% coverage: 3:265/290 of query aligns to 3:269/286 of 5u5gA

query
sites
5u5gA

V

V

G

G

F

F

I

I

G
|
G

L

L

G
|
G

D
x
R

Q
x
M

G

G

G

Q

P

A

M

I

A

C

R

R

M

R

I

L

V

L

D

A

A

S

G

Q

F

M

P

P

V

V

T

H

L

V

W

H

A
x
N

R
|
R

R
x
S

A

R

S

E

A
x
K

I

A

E

D

R

D

F

L

V

I

A

R

R

Q

G

G

A

A

G

V

V

W

A

A

A

P

D

D

P

I

A

V

A

A

L

L

A

T

S

R

A

A

C

A

E

R

L

V

V

L

C

F

L

V

C
|
C

V
x
T

T
x
A

G

G

D

S

A

E

D
x
A

V

V

R

Q

E

D

L
x
F

L

Y

-

H

-

A

I

P

D

D

R

R

G

G

M

L

I

L

A

A

A

C

L

L

R

E

K

V

R

G

S

D

L

I

V

V

A

V

I

D

H

L

S
|
S

T
|
T

I

I

N

A

P

P

K

E

T

T

C

A

V

E

E

G

L

L

A

H

R

A

M

A

V

F

S

A

A

Q

R

Q

G

G

A

A

T

D

L

Y

L

I

D

E

M

C

P

P

V

V

S
|
S

G
|
G

S
x
G

G

V

H

E

A

G

A

A

L

L

A

A

R

G

K

I

L

L

L

S

V

A

M

I

T

V

G

S

G

G

D

R

A

P

G

E

A

A

I

Y

A

G

R

L

T

I

M

R

P

P

V

L

L

L

E

E

S

V

Y

F

A

C

G

A

T

T

I

V

I

T

R

Y

M

V

G

P

D

E

V

P

G

G

A

K

A

A

M

Q

N

R

A

L

K
|
K

L

I

V

L

N

N

N
|
N

L

L

M

A

A
x
E

V

S

V

I

N

N

I

L

G

A

Q

G

A

A

F

I

H

E

A

V

L

I

A

S

L

Q

G

G

R

L

K

S

V

Q

G

G

V

L

E

D

P

L

A

K

A

S

L

M

R

A

Q

D

A

V

L

F

M

T

V

S

G

C

T

R

G

G

R

R

S

S

F

A

A
x
Y

I

M

D

D

-

V

-

A

L

L

I

G

A

Y

R

A

L

L

H

S

V

G

P

G

K

A

R

S

A

S

H

N

H
x
V

V

S

R

L

G

G

V
|
V

L

R

V

C

K
|
K

D

D

V

L

D

E

L

L

A

A

I

R

E

R

A

R

M

L

P

P

A

Q

G

D

E

Q

R

S

A

Y

Y

P

W

F

R

S

P

T

L

L

A

A

Q

M

A

T

G

T

L

F

D

D

A

T

L

V

E

R

Q

Q

binding (2-oxopropyl)phosphonic acid: S122 (= S120), G123 (= G121), G124 (≠ S122), K171 (= K169), N175 (= N173), E178 (≠ A176), Y215 (≠ A213)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G7 (= G7), G9 (= G9), R10 (≠ D10), M11 (≠ Q11), N30 (≠ A30), R31 (= R31), S32 (≠ R32), K35 (≠ A35), C63 (= C63), T64 (≠ V64), A65 (≠ T65), A69 (≠ D69), F73 (≠ L73), S95 (= S93), T96 (= T94), V232 (≠ H228), V236 (= V232), K239 (= K235)

3pduA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter sulfurreducens in complex with NADP+ (see paper)
32% identity, 70% coverage: 4:207/290 of query aligns to 5:209/287 of 3pduA

query
sites
3pduA

G

G

F

F

I

L

G
|
G

L

L

G
|
G

D
x
I

Q
x
M

G

G

P

P

M

M

A

A

R

A

M

N

I

L

V

V

D

R

A

A

G

G

F

F

P

D

V

V

T

T

L

V

W

W

A
x
N

R
|
R

R
x
N

A

P

S

A

A

K

I

C

E

A

R

P

F

L

V

V

A

A

R

L

G

G

A

A

G

R

V

Q

A

A

A

S

D

S

P

P

A

A

A

E

L

V

A

C

S

A

A

A

C

C

E

D

L

I

V

T

C

I

L

A

C
x
M

V
x
L

T
x
A

G

D

D

P

A

A

D
x
A

V

A

R

R

E

E

L

V

L

C

I

F

D

G

-

A

R

N

G

G

M

V

I

L

A

E

A

G

L

I

R

G

K

G

R

G

S

R

L

G

V

Y

A

I

I

D

H

M

S

S

T
|
T

I

V

N

D

P

D

K

E

T

T

C

S

V

T

E

A

L

I

A

G

R

A

M

A

V

V

S

T

A

A

R

R

G

G

A

G

T

R

L

F

L

L

D

E

M

A

P

P

V
|
V

S

S

G
|
G

S
x
T

G

K

H

K

A

P

A

A

L

E

A

D

R

G

K

T

L

L

L

I

V

I

M

L

T

A

G

A

G

G

D

D

A

Q

G

S

A

L

I

F

A

T

R

D

T

A

M

G

P

P

V

A

L

F

E

A

S

A

Y

L

A

G

G

K

T

K

I

C

I

L

R

H

M

L

G

G

D

E

V

V

G

G

A

Q

A

G

M

A

N

R

A

M

K
|
K

L

L

V

V

N

V

N

N

L

M

M

I

A

-

V

-

N

-

I

M

G

G

Q

Q

A

M

F

M

H

T

A

A

L

L

-

G

-

E

-

G

-

M

A

A

L

L

G

G

R

R

K

N

V

C

G

G

V

L

E

D

P

G

A

G

A

Q

L

L

R

L

Q

E

A

V

L

L

M

D

V

A

G

G

binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G7), G10 (= G9), I11 (≠ D10), M12 (≠ Q11), N31 (≠ A30), R32 (= R31), N33 (≠ R32), M64 (≠ C63), L65 (≠ V64), A66 (≠ T65), A70 (≠ D69), T96 (= T94), V121 (= V119), G123 (= G121), T124 (≠ S122), K171 (= K169)

Sites not aligning to the query:

3pefA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter metallireducens in complex with NADP+ (see paper)
29% identity, 82% coverage: 2:240/290 of query aligns to 3:229/287 of 3pefA

query
sites
3pefA

R

K

V

F

G

G

F

F

I

I

G

G

L

L

G
|
G

D
x
I

Q
x
M

G

G

G

S

P

A

M

M

A

A

R

K

M

N

I

L

V

V

D

K

A

A

G

G

F

C

P

S

V

V

T

T

L

I

W

W

A
x
N

R
|
R

R
x
S

A

P

S

E

A
x
K

I

A

E

E

R

E

F

L

V

A

A

A

R

L

G

G

A

A

G

E

V

R

A

A

D

T

P

P

A

C

A

E

L

V

A

V

S

E

A

S

C

C

E

P

L

V

V

T

C

F

L

A

C
x
M

V
x
L

T
x
A

G
x
D

D

P

A

A

D
x
A

V

A

R

E

E
|
E

L

V

L

C

I

F

D

G

R

K

-

H

G

G

M

V

I

L

A

E

A

G

L

I

R

G

K

E

R

G

S

R

L

G

V

Y

A

V

I

D

H

M

S

S

T
|
T

I

V

N

D

P

P

K

A

T

T

C

S

V

Q

E

R

L

I

A

G

R

V

M

A

V

V

S

V

A

A

R

K

G

G

A

G

T

R

L

F

L

L

D

E

M

A

P

P

V
|
V

S

S

G
|
G

S
|
S

G

K

H

K

A

P

A

A

L

E

A

D

R

G

K

T

L

L

L

I

V

I

M

L

T

A

G

A

G

G

D

D

A

R

G

N

A

L

I

Y

A

D

R

E

T

A

M

M

P

P

V

G

L

F

E

E

S

K

Y

M

A

G

G

K

T

K

I

I

R

H

M

L

G

G

D

D

V

V

G

G

A

K

A

G

M

A

N

E

A

M

K
|
K

L

L

V

V

N

V

N
|
N

L

M

M

V

A
x
M

V

G

N

M

I

M

G

A

Q

C

A

F

F

C

H

E

A

G

L

L

A

A

L

L

G

G

R

E

K

K

V

A

G

G

V

L

E

A

P

T

A

D

A

A

L

I

R

-

Q

-

A

-

L

-

M

-

V

-

G

-

T

-

G

-

R

-

S

-

F

-

A

-

I
x
L

D

D

L

V

I

I

A
x
G

R

A

L

G

H

A

V

M

P

A

K
x
N

R

P

A

M

H

F

H
x
A

V

L

R

K

G

G

V

G

L

L

V

I

K

R

D

D

V

R

D

N

L

F

A

A

binding glycerol: D67 (≠ G66), G123 (= G121), K171 (= K169), N175 (= N173), M178 (≠ A176), L203 (≠ I214), G207 (≠ A218), N213 (≠ K224), A217 (≠ H228)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G9), I11 (≠ D10), M12 (≠ Q11), N31 (≠ A30), R32 (= R31), S33 (≠ R32), K36 (≠ A35), M64 (≠ C63), L65 (≠ V64), A66 (≠ T65), A70 (≠ D69), E73 (= E72), T96 (= T94), V121 (= V119), G123 (= G121), S124 (= S122)

Sites not aligning to the query:

P0A9V8 3-sulfolactaldehyde reductase; SLA reductase; 4-hydroxybutyrate dehydrogenase; Gamma-hydroxybutyrate dehydrogenase; GHBDH; Succinic semialdehyde reductase; SSA reductase; EC 1.1.1.373; EC 1.1.1.61 from Escherichia coli (strain K12)
31% identity, 60% coverage: 3:177/290 of query aligns to 4:179/298 of P0A9V8

query
sites
P0A9V8

V

I

G

A

F

F

I

I

G

G

L

L

G

G

D
x
Q

Q
x
M

G

G

G

S

P

P

M

M

A

A

R

S

M

N

I

L

V

L

D

Q

A

Q

G

G

F

H

P

Q

V

L

T

R

L

V

W

F

A
x
D

R

V

R

N

A

A

S

E

A

A

I

V

E

R

R

H

F

L

V

V

A

D

R

K

G

G

A

A

G

T

V

P

A

A

D

N

P

P

A

A

A

Q

L

A

A

A

S

K

A

D

C

A

E

E

L

F

V

I

C

I

L

T

C

M

V
x
L

T

P

G

N

D

G

A

D

D

L

V

V

R

R

E

N

L

V

L

L

I

F

-

G

D

E

R

N

G

G

M

V

I

C

A

E

A

G

L

L

R

S

K

T

R

D

S

A

L

L

V

V

A

I

I

D

H

M

S

S

T
|
T

I

I

N

H

P

P

K

L

T

Q

C

T

V

D

E

K

L

L

A

I

R

A

M

D

V

M

S

Q

A

A

R

K

G

G

A

F

T

S

L

M

D

D

M

V

P

P

V

V

S
x
G

G
x
R

S
x
T

G

S

H

A

A

N

A

A

L

I

A

T

R

G

K

T

L

L

V

L

M

L

T

A

G

G

D

T

A

A

G

E

A

Q

I

V

A

E

R

R

T

A

M

T

P

P

V

I

L

L

E

M

S

A

Y

M

A

G

G

S

T

E

I

L

I

I

R

N

M

A

G

G

D

G

V

P

G

G

A

M

A

G

M

I

N

R

A

V

K

K

L

L

V

I

N
|
N

L
x
Y

M
|
M

A
x
S

V

I

QM 11:12 (≠ DQ 10:11) binding
D31 (≠ A30) binding
L65 (≠ V64) binding
T96 (= T94) binding
G122 (≠ S120) mutation to S: 25-fold decrease in catalytic efficiency with SLA as substrate. 5-fold decrease in catalytic efficiency with NADH as substrate.
R123 (≠ G121) binding ; mutation to G: 130-fold decrease in catalytic efficiency with SLA as substrate. 3-fold decrease in catalytic efficiency with NADH as substrate.
T124 (≠ S122) mutation to G: 230-fold decrease in catalytic efficiency with SLA as substrate. 12-fold decrease in catalytic efficiency with NADH as substrate.
NNYMS 174:178 (≠ NNLMA 172:176) binding

Sites not aligning to the query:

240 binding

6smyA Crystal structure of sla reductase yihu from e. Coli with nadh and product dhps
31% identity, 60% coverage: 3:177/290 of query aligns to 3:178/294 of 6smyA

query
sites
6smyA

V

I

G

A

F

F

I

I

G

G

L

L

G

G

D

Q

Q

M

G

G

G

S

P

P

M

M

A

A

R

S

M

N

I

L

V

L

D

Q

A

Q

G

G

F

H

P

Q

V

L

T

R

L

V

W

F

A

D

R

V

R

N

A

A

S

E

A

A

I

V

E

R

R

H

F

L

V

V

A

D

R

K

G

G

A

A

G

T

V

P

A

A

D

N

P

P

A

A

A

Q

L

A

A

A

S

K

A

D

C

A

E

E

L

F

V

I

C

I

L

T

C

M

V

L

T

P

G

N

D

G

A

D

D

L

V

V

R

R

E

N

L

V

L

L

I

F

-

G

D

E

R

N

G

G

M

V

I

C

A

E

A

G

L

L

R

S

K

T

R

D

S

A

L

L

V

V

A

I

I

D

H

M

S

S

T

T

I

I

N

H

P

P

K

L

T

Q

C

T

V

D

E

K

L

L

A

I

R

A

M

D

V

M

S

Q

A

A

R

K

G

G

A

F

T

S

L

M

D

D

M

V

P

P

V

V

S

G

G

R

S

T

G

S

H

A

A

N

A

A

L

I

A

T

R

G

K

T

L

L

V

L

M

L

T

A

G

G

D

T

A

A

G

E

A

Q

I

V

A

E

R

R

T

A

M

T

P

P

V

I

L

L

E

M

S

A

Y

M

A

G

G

S

T

E

I

L

I

I

R

N

M

A

G

G

D

G

V

P

G

G

A

M

A

G

M

I

N

R

A

V

K

K

L

L

V

I

N

N

L

Y

M

M

A

S

V

I

Sites not aligning to the query:

binding (2~{S})-2,3-bis(oxidanyl)propane-1-sulfonic acid: 232, 278

6smzC Crystal structure of sla reductase yihu from e. Coli in complex with nadh
31% identity, 60% coverage: 3:177/290 of query aligns to 3:178/295 of 6smzC

query
sites
6smzC

V

I

G

A

F

F

I

I

G

G

L

L

G
|
G

D
x
Q

Q
x
M

G

G

G

S

P

P

M

M

A

A

R

S

M

N

I

L

V

L

D

Q

A

Q

G

G

F

H

P

Q

V

L

T

R

L

V

W
x
F

A
x
D

R
x
V

R

N

A

A

S

E

A

A

I

V

E

R

R

H

F

L

V

V

A

D

R

K

G

G

A

A

G

T

V

P

A

A

D

N

P

P

A

A

A

Q

L

A

A

A

S

K

A

D

C

A

E

E

L

F

V

I

C

I

L

T

C
x
M

V
x
L

T

P

G

N

D

G

A

D

D

L

V

V

R

R

E

N

L
x
V

L

L

I

F

-

G

D

E

R

N

G

G

M

V

I

C

A

E

A

G

L

L

R

S

K

T

R

D

S

A

L

L

V

V

A

I

I

D

H

M

S
|
S

T
|
T

I

I

N

H

P

P

K

L

T

Q

C

T

V

D

E

K

L

L

A

I

R

A

M

D

V

M

S

Q

A

A

R

K

G

G

A

F

T

S

L

M

D

D

M

V

P

P

V

V

S

G

G
x
R

S

T

G

S

H

A

A

N

A

A

L

I

A

T

R

G

K

T

L

L

V

L

M

L

T

A

G

G

D

T

A

A

G

E

A

Q

I

V

A

E

R

R

T

A

M

T

P

P

V

I

L

L

E

M

S

A

Y

M

A

G

G

S

T

E

I

L

I

I

R

N

M

A

G

G

D

G

V

P

G

G

A

M

A

G

M

I

N

R

A

V

K

K

L

L

V

I

N

N

L

Y

M

M

A

S

V

I

binding nicotinamide-adenine-dinucleotide: G9 (= G9), Q10 (≠ D10), M11 (≠ Q11), F29 (≠ W29), D30 (≠ A30), V31 (≠ R31), M63 (≠ C63), L64 (≠ V64), V73 (≠ L73), S94 (= S93), T95 (= T94), R122 (≠ G121)

3q3cA Crystal structure of a serine dehydrogenase from pseudomonas aeruginosa pao1 in complex with NAD (see paper)
33% identity, 74% coverage: 2:216/290 of query aligns to 2:216/294 of 3q3cA

query
sites
3q3cA

R

Q

V

I

G

A

F

F

I

I

G

G

L

L

G
|
G

D
x
H

Q
x
M

G

G

G

A

P

P

M

M

A

A

R

T

M

N

I

L

V

L

D

K

A

A

G

G

F

Y

P

L

V

L

T

N

L

V

W
x
F

A
x
D

R
x
L

R

V

A

Q

S

S

A

A

I

V

E

D

R

G

F

L

V

V

A

A

R

A

G

G

A

A

G

S

V

A

A

A

A

R

D

S

P

A

A

R

A

D

L

A

A

V

S

Q

A

G

C

A

E

D

L

V

V

V

C

I

L

S

C
x
M

V
x
L

T
x
P

G

A

D

S

A

Q

D

H

V

V

R

E

E

G

L

L

L

Y

I

L

D

D

R

D

G

G

M

L

I

L

A

A

A

H

L

I

R

A

K

P

R

G

S

T

L

L

V

V

A

L

I

E

H

C

S

S

T
|
T

I

I

N

A

P

P

K

T

T

S

C

A

V

R

E

K

L

I

A

H

R

A

M

A

V

A

S

R

A

E

R

R

G

G

A

L

T

A

L

M

L

L

D

D

M

A

P

P

V
|
V

S

S

G
|
G

S

G

G

T

H

A

A

G

A

A

L

A

A

A

R

G

K

T

L

L

L

T

V

F

M

M

T

V

G

G

D

D

A

A

G

E

A

A

I

L

A

E

R

K

T

A

M

R

P

P

V

L

L

F

E

E

S

A

Y

M

A

G

G

R

T

N

I

I

I

F

R

H

M

A

G

G

D

P

V

D

G

G

A

A

A

G

M

Q

N

V

A

A

K

K

L

V

V

C

N

N

-

Q

L

L

M

L

A

A

V

V

V

L

N

M

I

I

G

G

Q

T

A

A

F

-

H

E

A

A

L

M

A

A

L

L

G

G

R

V

K

A

V

N

G

G

V

L

E

E

P

A

A

K

A

V

L

L

R

A

Q

E

A

I

L

M

M

R

V

R

G

S

T

S

G

G

R

G

S

N

F

W

A

A

I

L

D

E

L

V

binding nicotinamide-adenine-dinucleotide: G9 (= G9), H10 (≠ D10), M11 (≠ Q11), F29 (≠ W29), D30 (≠ A30), L31 (≠ R31), M63 (≠ C63), L64 (≠ V64), P65 (≠ T65), T94 (= T94), V119 (= V119), G121 (= G121)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 237, 244

2uyyA Structure of the cytokine-like nuclear factor n-pac
26% identity, 83% coverage: 2:241/290 of query aligns to 8:250/292 of 2uyyA

query
sites
2uyyA

R

K

V

I

G

G

F

F

I

L

G

G

L

L

G
|
G

D
x
L

Q
x
M

G

G

G

S

P

G

M

I

A

V

R

S

M

N

I

L

V

L

D

K

A

M

G

G

F

H

P

T

V

V

T

T

L

V

W

W

A
x
N

R
|
R

R
x
T

A

A

S

E

A

K

I

C

E

D

R

L

F

F

V

I

A

Q

R

E

G

G

A

A

G

R

V

L

A

G

A

R

D

T

P

P

A

A

A

E

L

V

A

V

S

S

A

T

C

C

E

D

L

I

V

T

C

F

L

A

C

C

V
|
V

T
x
S

G

D

D

P

A

K

D
x
A

V

A

R

K

E

D

L

L

L

V

I

L

-

G

D

P

R

S

G

G

M

V

I

L

A

Q

A

G

L

I

R

R

K

P

R

G

S

K

L

C

V

Y

A

V

I

D

H

M

S

S

T
|
T

I

V

N

D

P

A

K

D

T

T

C

V

V

T

E

E

L

L

A

A

R

Q

M

V

V

I

S

V

A

S

R

R

G

G

A

G

T

R

L

F

L

L

D

E

M

A

P

P

V

V

S

S

G

G

S

N

G

Q

H

Q

A

L

A

S

L

N

A

D

R

G

K

M

L

L

L

V

V

I

M

L

T

A

G

A

G

G

D

D

A

R

G

G

A

L

I

Y

A

E

R

D

T

C

M

S

P

S

V

C

L

F

E

Q

S

A

Y

M

A

G

G

K

T

T

I

S

I

F

R

F

M

L

G

G

D

E

V

V

G

G

A

N

A

A

M

A

N

K

A

M

K

M

L

L

V

I

N

V

N

N

L

M

M

V

A

Q

V

G

V

S

N

F

I

M

G

A

Q

T

A

I

F

A

H

E

A

G

L

L

A

T

L

L

G

A

R

Q

K

V

V

T

G

G

V

Q

E

S

P

Q

A

Q

A

T

L

L

R

L

Q

D

A

I

L

L

M

N

V

Q

G

G

T

Q

G

L

R

A

S

S

F

I

A

F

I

L

D

D

L

Q

I

K

A

C

R

Q

-

N

-

I

L

L

H

Q

V

G

P

N

K

F

R

K

A

P

H

D

H
x
F

V
x
Y

R

L

G

K

V
x
Y

L

I

V

Q

K
|
K

D

D

V

L

D

R

L

L

A

A

I

I

binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4s)-3,4-dihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: G15 (= G9), L16 (≠ D10), M17 (≠ Q11), N36 (≠ A30), R37 (= R31), T38 (≠ R32), V70 (= V64), S71 (≠ T65), A75 (≠ D69), T101 (= T94), F237 (≠ H228), Y238 (≠ V229), Y241 (≠ V232), K244 (= K235)

1wp4A Structure of tt368 protein from thermus thermophilus hb8 (see paper)
30% identity, 83% coverage: 2:241/290 of query aligns to 2:239/288 of 1wp4A

query
sites
1wp4A

R

K

V

V

G

A

F

F

I

I

G
|
G

L
|
L

G
|
G

D
x
A

Q
x
M

G

G

G

Y

P

P

M

M

A

A

R

G

M

H

I

L

V

A

D

R

A

R

G

-

F

F

P

P

V

T

T

L

L

V

W

W

A
x
N

R
|
R

R
x
T

A

F

S

E

A
x
K

I

A

E

L

R

R

F

H

V

Q

A

E

R

E

G

-

A

F

G

G

V

S

A

E

A

A

D

V

P

P

A

L

A

E

L

R

A

V

S

A

A

E

C

A

E

R

L

V

V

I

C

F

L

T

C
|
C

V
x
L

T
x
P

G

T

D

T

A

R

D
x
E

V

V

R

Y

E

E

L

-

I

V

D

A

R

E

G

A

M

L

I

Y

A

P

A

Y

L

L

R

R

K

E

R

G

S

T

L

Y

V

W

A

V

I

D

H

A

S

T

T
x
S

I

G

N

E

P

P

K

E

T

A

C

S

V

R

E

R

L

L

A

A

R

E

M

R

V

L

S

R

A

E

R

K

G

G

A

V

T

T

L

Y

L

L

D

D

M

A

P

P

V
|
V

S
|
S

G
|
G

S
x
G

G

T

H

S

A

G

A

A

L

E

A

A

R

G

K

T

L

L

L

T

V

V

M

M

T

L

G

G

D

P

A

E

G

E

A

A

I

V

A

E

R

R

T

V

M

R

P

P

V

F

L

L

E

-

S

A

Y

Y

A

A

G

K

T

K

I

V

R

H

M

V

G

G

D

P

V

V

G

G

A

A

A

G

M

H

N

A

A

V

K
|
K

L

A

V

I

N
|
N

L

A

M

L

A

L

V

A

V

V

N

N

I

L

G

W

Q

A

A

A

F

G

H

E

A

G

L

L

A

L

L

A

G

L

R

V

K

K

V

Q

G

G

V

V

E

S

P

A

A

E

A

K

L

A

R

L

Q

E

A

V

L

I

M

N

V

A

G

S

T

S

G

G

R

R

S

S

F

N

A

A

I

T

D

E

L

N

I

L

A

I

R

P

L

Q

H

R

V

V

P

L

K

T

R

R

A

A

H

F

-

P

-

K

-
x
T

H
x
F

V

A

R

L

G

G

V

L

L

L

V

V

K
|
K

D

D

V

L

D

G

L

I

A

A

I

M

active site: S116 (= S120), K164 (= K169), N167 (= N172), N168 (= N173)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G7), L8 (= L8), G9 (= G9), A10 (≠ D10), M11 (≠ Q11), N29 (≠ A30), R30 (= R31), T31 (≠ R32), K34 (≠ A35), C61 (= C63), L62 (≠ V64), P63 (≠ T65), E67 (≠ D69), S90 (≠ T94), V115 (= V119), T225 (vs. gap), F226 (≠ H228), K233 (= K235)
binding sulfate ion: S116 (= S120), G117 (= G121), G118 (≠ S122), K164 (= K169)

2cvzC Structure of hydroxyisobutyrate dehydrogenase from thermus thermophilus hb8 (see paper)
30% identity, 83% coverage: 2:241/290 of query aligns to 3:240/289 of 2cvzC

query
sites
2cvzC

R

K

V

V

G

A

F

F

I

I

G
|
G

L
|
L

G
|
G

D
x
A

Q
x
M

G

G

G

Y

P

P

M

M

A

A

R

G

M

H

I

L

V

A

D

R

A

R

G

-

F

F

P

P

V

T

T

L

L

V

W

W

A
x
N

R
|
R

R
x
T

A

F

S

E

A

K

I

A

E

L

R

R

F

H

V

Q

A

E

R

E

G

-

A

F

G

G

V

S

A

E

A

A

D

V

P

P

A

L

A

E

L

R

A

V

S

A

A

E

C

A

E

R

L

V

V

I

C

F

L

T

C
|
C

V
x
L

T
x
P

G

T

D

T

A

R

D
x
E

V

V

R

Y

E
|
E

L

-

I

V

D

A

R

E

G

A

M

L

I

Y

A

P

A

Y

L

L

R

R

K

E

R

G

S

T

L

Y

V

W

A

V

I

D

H

A

S

T

T
x
S

I

G

N

E

P

P

K

E

T

A

C

S

V

R

E

R

L

L

A

A

R

E

M

R

V

L

S

R

A

E

R

K

G

G

A

V

T

T

L

Y

L

L

D

D

M

A

P

P

V
|
V

S
|
S

G

G

S

G

G

T

H

S

A

G

A

A

L

E

A

A

R

G

K

T

L

L

L

T

V

V

M

M

T

L

G

G

D

P

A

E

G

E

A

A

I

V

A

E

R

R

T

V

M

R

P

P

V

F

L

L

E

-

S

A

Y

Y

A

A

G

K

T

K

I

V

R

H

M

V

G

G

D

P

V

V

G

G

A

A

A

G

M

H

N

A

A

V

K
|
K

L

A

V

I

N
|
N

L

A

M

L

A

L

V

A

V

V

N

N

I

L

G

W

Q

A

A

A

F

G

H

E

A

G

L

L

A

L

L

A

G

L

R

V

K

K

V

Q

G

G

V

V

E

S

P

A

A

E

A

K

L

A

R

L

Q

E

A

V

L

I

M

N

V

A

G

S

T

S

G

G

R

R

S

S

F

N

A

A

I

T

D

E

L

N

I

L

A

I

R

P

L

Q

H

R

V

V

P

L

K

T

R

R

A

A

H

F

-

P

-

K

-

T

H
x
F

V

A

R

L

G

G

V

L

L

L

V

V

K
|
K

D

D

V

L

D

G

L

I

A

A

I

M

active site: S117 (= S120), K165 (= K169), N168 (= N172), N169 (= N173)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G7), L9 (= L8), G10 (= G9), A11 (≠ D10), M12 (≠ Q11), N30 (≠ A30), R31 (= R31), T32 (≠ R32), C62 (= C63), L63 (≠ V64), P64 (≠ T65), E68 (≠ D69), E71 (= E72), S91 (≠ T94), V116 (= V119), F227 (≠ H228), K234 (= K235)

Q49A26 Cytokine-like nuclear factor N-PAC; NPAC; 3-hydroxyisobutyrate dehydrogenase-like protein; Glyoxylate reductase 1 homolog; Nuclear protein NP60; Nuclear protein of 60 kDa; Nucleosome-destabilizing factor; hNDF; Putative oxidoreductase GLYR1 from Homo sapiens (Human) (see 3 papers)
26% identity, 74% coverage: 2:215/290 of query aligns to 269:483/553 of Q49A26

query
sites
Q49A26

R

K

V

I

G
|
G

F
|
F

I
x
L

G
|
G

L
|
L

G
|
G

D
x
L

Q
x
M

G
|
G

G
x
S

P
x
G

M
x
I

A
x
V

R
x
S

M
x
N

I

L

V

L

D

K

A

M

G

G

F

H

P

T

V

V

T

T

L

V

W

W

A

N

R

R

R

T

A

A

S

E

A

K

I

C

E

D

R

L

F

F

V

I

A

Q

R

E

G

G

A

A

G

R

V

L

A

G

A

R

D

T

P

P

A

A

A

E

L

V

A

V

S

S

A

T

C

C

E

D

L

I

V

T

C

F

L

A

C

C

V

V

T

S

G

D

D

P

A

K

D

A

V

A

R

K

E

D

L

L

L

V

I

L

-

G

D

P

R

S

G

G

M

V

I

L

A

Q

A

G

L

I

R

R

K

P

R

G

S

K

L

C

V

Y

A

V

I

D

H

M

S

S

T
|
T

I

V

N

D

P

A

K

D

T

T

C

V

V

T

E

E

L

L

A

A

R

Q

M

V

V

I

S

V

A

S

R

R

G

G

A

G

T

R

L

F

L

L

D

E

M

A

P

P

V

V

S

S

G

G

S

N

G

Q

H

Q

A

L

A

S

L

N

A

D

R

G

K

M

L

L

L

V

V

I

M

L

T

A

G

A

G

G

D

D

A

R

G

G

A

L

I

Y

A

E

R

D

T

C

M

S

P

S

V

C

L

F

E

Q

S

A

Y

M

A

G

G

K

T

T

I

S

I

F

R

F

M

L

G

G

D

E

V

V

G

G

A

N

A

A

M

A

N

K

A

M

K
x
M

L

L

V

I

N

V

N

N

L

M

M

V

A

Q

V

G

V

S

N

F

I

M

G

A

Q

T

A

I

F

A

H

E

A

G

L

L

A

T

L

L

G

A

R

Q

K

V

V

T

G

G

V

Q

E

S

P

Q

A

Q

A

T

L

L

R

L

Q

D

A

I

L

L

M

N

V

Q

G

G

T

Q

G

L

R

A

S

S

F

I

A

F

I

L

D

D

271:285 (vs. 4:18, 40% identical) binding
T362 (= T94) binding
M437 (≠ K169) mutation to K: Loss of tetramerization and protein stability.; mutation to N: No effect on tetramerization or protein stability.

Sites not aligning to the query:

214 D→A: Slightly reduced stimulation of KDM1B demethylase activity, but normal KDM1B-binding.
214:217 Interaction with histone H3
216 H→A: Slightly reduced stimulation of KDM1B demethylase activity, but normal KDM1B-binding.
216:225 Interaction with KDM1B
217 Required to promote KDM1B demethylase activity toward histone H3K4me1 and H3K4me2; F→A: Abolished stimulation of KDM1B demethylase activity, reduced affinity for histone H3 of the dimer with KDM1B, but normal KDM1B-binding.
219 H→A: Impaired KDM1B-binding and abolished stimulation of KDM1B demethylase activity; when associated with A-223.
220:222 FLL→AAA: Impaired KDM1B-binding and abolished stimulation of KDM1B demethylase activity.
223 S→A: Impaired KDM1B-binding and abolished stimulation of KDM1B demethylase activity; when associated with A-219.
496 P → L: decreased interaction with GATA4; decreased synergistic activation of GATA4 target genes transcription; detrimental effect on cardiomyocyte differentiation
505 binding

Q9I5I6 NAD-dependent L-serine dehydrogenase; L-serine 3-dehydrogenase (NAD(+)); EC 1.1.1.387 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
33% identity, 74% coverage: 2:216/290 of query aligns to 3:218/298 of Q9I5I6

query
sites
Q9I5I6

R
x
Q

V
x
I

G
x
A

F
|
F

I
|
I

G
|
G

L
|
L

G
|
G

D
x
H

Q
x
M

G
|
G

G
x
A

P
|
P

M
|
M

A
|
A

R
x
T

M
x
N

I
x
L

V
x
L

D
x
K

A
|
A

G
|
G

F
x
Y

P
x
L

V
x
L

T
x
N

L
x
V

W
x
F

A
x
D

R

L

R

V

A

Q

S

S

A

A

I

V

E

D

R

G

F

L

V

V

A

A

R

A

G

G

A

A

G

S

V

A

A

A

A

R

D

S

P

A

A

R

A

D

L

A

A

V

S

Q

A

G

C

A

E

D

L

V

V

V

C

I

L

S

C

M

V

L

T
x
P

G

A

D

S

A

Q

D

H

V

V

R

E

E

G

L

L

L

Y

I

L

D

D

R

D

-

D

G

G

M

L

I

L

A

A

A

H

L

I

R

A

K

P

R

G

S

T

L

L

V

V

A

L

I

E

H

C

S

S

T
|
T

I

I

N

A

P

P

K

T

T

S

C

A

V

R

E

K

L

I

A

H

R

A

M

A

V

A

S

R

A

E

R

R

G

G

A

L

T

A

L

M

L

L

D

D

M

A

P

P

V

V

S
|
S

G

G

S

G

G

T

H

A

A

G

A

A

L

A

A

A

R

G

K

T

L

L

L

T

V

F

M

M

T

V

G

G

D

D

A

A

G

E

A

A

I

L

A

E

R

K

T

A

M

R

P

P

V

L

L

F

E

E

S

A

Y

M

A

G

G

R

T

N

I

I

I

F

R

H

M

A

G

G

D

P

V

D

G

G

A

A

A

G

M

Q

N

V

A

A

K
|
K

L

V

V

C

N

N

N
|
N

-

Q

L

L

M

L

A

A

V

V

V

L

N

M

I

I

G

G

Q

T

A

A

F

-

H

E

A

A

L

M

A

A

L

L

G

G

R

V

K

A

V

N

G

G

V

L

E

E

P

A

A

K

A

V

L

L

R

A

Q

E

A

I

L

M

M

R

V

R

G

S

T

S

G

G

R

G

S

N

F
x
W

A

A

I

L

D

E

L

V

P66 (≠ T65) binding
T96 (= T94) binding ; mutation to A: Almost abolished activity.
S122 (= S120) mutation to A: Strongly reduced activity.
K171 (= K169) active site
N175 (= N173) mutation to A: Strongly reduced activity.
W214 (≠ F212) mutation to A: Almost abolished activity.

Sites not aligning to the query:

2:31 binding
219 Y→A: Strongly reduced activity.
246 binding ; K→A: Almost abolished activity.
247 D→A: Almost abolished activity.

3obbA Crystal structure of a possible 3-hydroxyisobutyrate dehydrogenase from pseudomonas aeruginosa pao1 (see paper)
33% identity, 74% coverage: 2:216/290 of query aligns to 3:217/295 of 3obbA

query
sites
3obbA

R

Q

V

I

G

A

F

F

I

I

G

G

L

L

G

G

D

H

Q

M

G

G

G

A

P

P

M

M

A

A

R

T

M

N

I

L

V

L

D

K

A

A

G

G

F

Y

P

L

V

L

T

N

L

V

W

F

A

D

R

L

R

V

A

Q

S

S

A

A

I

V

E

D

R

G

F

L

V

V

A

A

R

A

G

G

A

A

G

S

V

A

A

A

A

R

D

S

P

A

A

R

A

D

L

A

A

V

S

Q

A

G

C

A

E

D

L

V

V

V

C

I

L

S

C

M

V

L

T

P

G

A

D

S

A

Q

D

H

V

V

R

E

E

G

L

L

L

Y

I

L

D

D

R

D

G

D

M

G

I

L

A

L

A

A

L

H

R

I

K

A

R

P

S

T

L

L

V

V

A

L

I

E

H

C

S

S

T

T

I

I

N

A

P

P

K

T

T

S

C

A

V

R

E

K

L

I

A

H

R

A

M

A

V

A

S

R

A

E

R

R

G

G

A

L

T

A

L

M

L

L

D

D

M

A

P

P

V

V

S

S

G
|
G

S
x
G

G

T

H

A

A

G

A

A

L

A

A

A

R

G

K

T

L

L

L

T

V

F

M

M

T

V

G

G

D

D

A

A

G

E

A

A

I

L

A

E

R

K

T

A

M

R

P

P

V

L

L

F

E

E

S

A

Y

M

A

G

G

R

T

N

I

I

I

F

R

H

M

A

G

G

D

P

V

D

G

G

A

A

A

G

M

Q

N

V

A

A

K
|
K

L

V

V

C

N

N

N
|
N

-

Q

L

L

M

L

A

A

V

V

V

L

N

M

I

I

G

G

Q

T

A

A

F

-

H

E

A

A

L

M

A

A

L

L

G

G

R

V

K

A

V

N

G

G

V

L

E

E

P

A

A

K

A

V

L

L

R

A

Q

E

A

I

L

M

M

R

V

R

G

S

T

S

G

G

R

G

S

N

F
x
W

A

A

I

L

D

E

L

V

binding 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid: G122 (= G121), G123 (≠ S122), K170 (= K169), N174 (= N173), W213 (≠ F212)

Sites not aligning to the query:

binding 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid: 218, 238

7puaFM uS11m (see paper)
27% identity, 73% coverage: 1:211/290 of query aligns to 6:230/327 of 7puaFM

query
sites
7puaFM

M

L

R

N

V

V

G

G

F

V

I

V

G

G

L

M

G

G

D

N

Q

M

G

G

G

V

P

P

M

I

A

A

R

R

M

N

I

L

V

-

D

-

A

-

G

G

F

F

P

K

V

-

T

-

L

-

W

-

A

A

R

R

R

S

A

A

S

M

A

Y

I

L

E

Q

-

I

-

H

-

S

R

R

F

A

V

L

A

S

R

K

G

A

A

K

G

R

V

V

A

C

A

D

D

D

-

L

-

S

-

V

-

D

-

G

-

A

-

T

-

C

-

A

-

M

-

R

-

I

-

H

-

D

-

R

P

Y

A

S

A

T

L

M

A

T

S

K

A

W

C

C

E

D

L

V

V

I

C

V

L

L

C

A

V

L

T

A

G

D

D

V

A

K

D

A

V

S

R

R

E

H

L

A

L

L

I

L

-

E

-

D

D

N

R

E

G

S

M

L

I

I

A

M

A

N

L

A

R

R

K

K

R

G

S

Q

L

I

V

I

A

V

I

D

H

H

S

T

T

T

I

V

N

D

P

A

K

E

T

T

C

S

V

R

E

E

L

C

A

A

R

H

M

E

V

A

S

A

A

R

R

R

G

G

A

A

T

Y

L

F

L

L

D

D

M

A

P

P

V

M

S

S

G

G

S

S

G

P

H

R

A

A

A

C

L

F

A

N

R

G

K

Q

L

L

V

L

M

M

T

V

G

G

D

D

A

A

G

E

A

T

I

F

A

Q

R

K

T

M

M

L

P

P

V

I

L

F

E

H

S

M

Y

Y

A

A

G

D

T

N

I

V

I

F

R

H

M

M

G

G

D

E

V

S

G

G

A

S

A

G

M

T

N

A

A

A

K

K

L

M

V

I

N

S

N

Q

L

A

M

L

A

V

V

A

V

S

N

H

I

N

G

A

Q

A

A

A

F

A

H

E

A

A

L

L

A

S

L

M

G

A

R

H

K

A

V

L

G

G

V

I

E

E

-

D

P

Q

A

S

A

K

L

L

R

V

Q

Q

A

V

L

L

M

D

V

A

G

S

T

W

G

G

R

S

S

S

Sites not aligning to the query:

binding : 269, 289

5y8hA Mycobacterium tuberculosis 3-hydroxyisobutyrate dehydrogenase (mthibadh) + NAD+ (see paper)
33% identity, 73% coverage: 3:214/290 of query aligns to 3:212/291 of 5y8hA

query
sites
5y8hA

V

I

G

A

F

F

I

L

G
|
G

L

L

G
|
G

D
x
N

Q
x
M

G

G

G

A

P

P

M

M

A

S

R

A

M

N

I

L

V

V

D

G

A

A

G

G

F

H

P

V

V

V

T

R

L

G

W
x
F

A
x
D

R
x
P

R

A

A

P

S

T

A

A

I

A

E

S

R

G

F

A

V

A

A

A

R

H

G

G

A

V

G

A

V

V

A

F

-

R

A

S

D

A

P

P

A

E

A

A

L

V

A

A

S

E

A

A

C

D

E

V

L

V

V

I

C

T

L
x
M

C
x
L

V

P

T

T

G

G

D

E

A

V

D

-

V

V

R

R

E

R

L

C

L

Y

I

T

D

D

R

-

G

-

M

V

I

L

A

A

L

A

R

R

K

P

R

A

S

T

L

L

V

F

A

I

I

D

H

S

S

S

T

T

I

I

N

S

P

V

K

T

T

D

C

A

V

R

E

E

L

V

A

H

R

A

M

L

V

A

S

E

A

S

R

H

G

G

A

M

T

L

L

Q

L

L

D

D

M

A

P

P

V

V

S

S

G

G

S
x
G

G

V

H

K

A

G

A

A

L

A

A

A

R

A

K

T

L

L

L

A

V

F

M

M

T

V

G

G

D

D

A

E

G

S

A

T

I

L

A

R

R

R

T

A

M
x
R

P

P

V

V

L

L

E
|
E

S

P

Y

M

A
|
A

G

G

T
x
K

I

I

R

H

M

C

G

G

D

A

V

A

G

G

A

A

A

G

M

Q

N

A

A

A

K

K

L

V

V

C

N

N

L

M

M

V

A

L

V

A

V

V

N

Q

I

Q

G

I

Q

A

A

I

F

A

H

E

A

A

L

F

A

V

L

L

G

A

R

E

K

K

V

L

G

G

V

L

E

S

P

A

A

Q

A

S

L

L

R

F

Q

D

A

V

L

I

M

T

V

G

G

A

T

T

G

G

R

N

S

C

F

W

A

A

I

V

binding (2~{S})-2-methylpentanedioic acid: R144 (≠ M146), E148 (= E150), A151 (= A153), K153 (≠ T155)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G9 (= G9), N10 (≠ D10), M11 (≠ Q11), F29 (≠ W29), D30 (≠ A30), P31 (≠ R31), M63 (≠ L62), L64 (≠ C63), G120 (≠ S122)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 239, 242

Query Sequence

>Ga0059261_2836 Ga0059261_2836 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases
MRVGFIGLGDQGGPMARMIVDAGFPVTLWARRASAIERFVARGAGVAADPAALASACELV
CLCVTGDADVRELLIDRGMIAALRKRSLVAIHSTINPKTCVELARMVSARGATLLDMPVS
GSGHAALARKLLVMTGGDAGAIARTMPVLESYAGTIIRMGDVGAAMNAKLVNNLMAVVNI
GQAFHALALGRKVGVEPAALRQALMVGTGRSFAIDLIARLHVPKRAHHVRGVLVKDVDLA
IEAMPAGERAYWRPLAQAGLDALEQLISGGVTLLPVKEEEHGEYVVYGTA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory