SitesBLAST

A9 (≠ P6) binding
NPFC 39:42 (≠ NPFD 36:39) binding
C66 (≠ I63) binding
123:134 (vs. 116:127, 92% identical) binding
D128 (= D121) to N: in GA2B; decreased protein stability; dbSNP:rs104894678
T154 (≠ E147) to M: in dbSNP:rs1130426
R164 (= R157) to Q: in GA2B; reduced electron transfer activity; dbSNP:rs104894677
E165 (= E158) mutation E->A,Q: Drastically increases interprotein electron transfer rates.
183:205 (vs. 176:198, 83% identical) Recognition loop
L195 (= L188) mutation to A: Severely impaired in complex formation with ACADM.
K200 (= K193) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
KAK 200:202 (= KAK 193:195) mutation to RAR: Does not abolish methylation by ETFBKMT.
KAKK 200:203 (≠ KAKS 193:196) mutation to RAKR: Does not abolish electron transfer activity. Abolishes sensitivity to inhibition by lysine methyltransferase ETFBKMT.
K203 (≠ S196) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.

2a1uB Crystal structure of the human etf e165betaa mutant (see paper)
58% identity, 99% coverage: 1:246/249 of query aligns to 1:250/252 of 2a1uB

query
sites
2a1uB

M

L

K

R

I

V

L

L

V

V

P
x
A

V
|
V

K
|
K

R

R

V

V

L

I

D

D

Y

Y

N

A

V

V

K

K

P

I

R

R

V

V

K

K

A

P

D

D

G

R

T

T

G

G

V

V

D

V

L

T

A

D

N

G

V

V

K

K

M

H

S

S

M

M

N
|
N

P
|
P

F
|
F

D
x
C

E

E

I

I

A

A

V

V

E

E

A

A

I

V

R

R

L

L

K

K

E

E

K

K

G

K

A

L

A

V

T

K

E

E

I

V

V

I

V

A

V

V

S
|
S

I
x
C

G

G

E

P

Q

A

K

Q

A

C

Q

Q

E

E

T

T

L

I

R

R

T

T

A

A

L

L

A

A

M

M

G

G

A

A

D

D

R

R

A

G

I

I

L

H

I

V

-

E

-

V

-

P

V

A

A

E

E

A

D

E

K

R

V

L

E

G

P
|
P

L

L

G

Q

V
|
V

A

A

K

R

L

V

L

L

A

A

K

K

V

L

A

A

E

E

A

K

E

E

A

K

P

V

G

D

L

L

I

V

I

L

L
|
L

G
|
G

K
|
K

Q
|
Q

A
|
A

I

I

D

D

D
|
D

D

D

N
x
C

N
|
N

Q
|
Q

T
|
T

G

G

Q

Q

M

M

L

T

G

A

A

G

L

F

L

L

G

D

W

W

G

P

Q

Q

G

G

T

T

F

F

A

A

N

S

K

Q

V

V

E

T

I

L

A

E

G

G

E

D

Q

K

V

L

D

K

V

V

T

E

R

R

E

A

V

I

D

D

G

G

L

L

E

E

T

T

V

L

K

R

L

L

K

K

L

L

P

P

A

A

I

V

V

V

T

T

T

A

D

D

L
|
L

R

R

L

L

N

N

E

E

P

P

R

R

Y

Y

A

A

S

T

L

L

P

P

N

N

I

I

M

M

K

K

A

A

K

K

S

K

K

K

P

K

L

I

D

E

Q

V

K

I

T

K

P

P

A

G

D

D

Y

L

G

G

V

V

D

D

V

L

A

T

P

S

R

K

L

L

T

S

V

V

V

I

S

S

V

V

T

E

E

D

P

P

A

P

K

Q

R

R

Q

T

A

A

G

G

V

V

K

K

V

V

A

E

D

T

V

T

D

E

E

D

L

L

V

V

G

A

K

K

L

L

K

K

A

E

L

I

G

G

binding adenosine monophosphate: A6 (≠ P6), V7 (= V7), K8 (= K8), N36 (= N36), C39 (≠ D39), S62 (= S62), C63 (≠ I63), P98 (= P94), V101 (= V97), L119 (= L115), G120 (= G116), K121 (= K117), Q122 (= Q118), A123 (= A119), D126 (= D122), C128 (≠ N124), N129 (= N125), Q130 (= Q126), T131 (= T127)
binding flavin-adenine dinucleotide: P37 (= P37), F38 (= F38), L182 (= L178)

Q9DCW4 Electron transfer flavoprotein subunit beta; Beta-ETF from Mus musculus (Mouse) (see paper)
58% identity, 99% coverage: 1:246/249 of query aligns to 4:253/255 of Q9DCW4

query
sites
Q9DCW4

M

L

K

R

I

A

L

L

V

V

P

A

V

V

K

K

R

R

V

V

L

I

D

D

Y

F

N

A

V

V

K

K

P

I

R

R

V

V

K

K

A

P

D

D

G

K

T

S

G

G

V

V

D

V

L

T

A

D

N

G

V

V

K

K

M

H

S

S

M

M

N

N

P

P

F

F

D

C

E

E

I

I

A

A

V

V

E

E

A

A

I

V

R

R

L

L

K

K

E

E

K

K

G

K

A

L

A

V

T

K

E

E

I

I

V

I

V

A

V

V

S

S

I

C

G

G

E

P

Q

S

K

Q

A

C

Q

Q

E

E

T

T

L

I

R

R

T

T

A

A

L

L

A

A

M

M

G

G

A

A

D

D

R

R

A

G

I

I

L

H

I

V

-

E

-

I

-

P

-

G

V

A

A

Q

E

A

D

E

K

S

V

L

E

G

P

P

L

L

G

Q

V

V

A

A

K

R

L

V

L

L

A

A

K

K

V

L

A

A

E

E

A

K

E

E

A

K

P

V

G

D

L

L

I

L

I

F

L

L

G

G

K

K

Q

Q

A

A

I

I

D

D

N

C

N

N

Q

Q

T

T

G

G

Q

Q

M

M

L

T

G

A

A

G

L

L

G

D

W

W

G

P

Q

Q

G

G

T

T

F

F

A

A

N

S

K

Q

V

V

E

T

I

L

A

E

G

G

E

D

Q

K

V

V

D

K

V

V

T

E

R

R

E

E

V

I

D

D

G

G

L

L

E

E

T

T

V

L

K

R

L

L

K

K

L

L

P

P

A

A

I

V

V

V

T

T

T

A

D

D

L

L

R

R

L

L

N

N

E

E

P

P

R

R

Y

Y

A

A

S

T

L

L

P

P

N

N

I

I

M

M

K
|
K

A

A

K

K

S
x
K

K

K

P

K

L

I

D

E

Q

V

K

V

T

K

P

A

A

G

D

D

Y

L

G

G

V

V

D

D

V

L

A

T

P

S

R

K

L

V

T

S

V

V

V

I

S

S

V

V

T

E

E

E

P

P

A

P

K

Q

R

R

Q

S

A

A

G

G

V

V

K

K

V

V

A

E

D

T

V

T

D

E

E

D

L

L

V

V

G

A

K

K

L

L

K

K

A

E

L

V

G

G

K200 (= K193) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
K203 (≠ S196) modified: N6,N6,N6-trimethyllysine; by ETFBKMT

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

Q2TBV3 Electron transfer flavoprotein subunit beta; Beta-ETF from Bos taurus (Bovine) (see paper)
59% identity, 99% coverage: 1:246/249 of query aligns to 4:253/255 of Q2TBV3

query
sites
Q2TBV3

M

L

K

R

I

A

L

L

V

V

P

A

V

V

K

K

R

R

V

V

L

I

D

D

Y

F

N

A

V

V

K

K

P

I

R

R

V

V

K

K

A

P

D

D

G

K

T

T

G

G

V

V

D

V

L

T

A

D

N

G

V

V

K

K

M

H

S

S

M

M

N

N

P

P

F

F

D

C

E

E

I

I

A

A

V

V

E

E

A

A

I

V

R

R

L

L

K

K

E

E

K

K

G

K

A

L

A

V

T

K

E

E

I

I

V

I

V

A

V

V

S

S

I

C

G

G

E

P

Q

A

K

Q

A

C

Q

Q

E

E

T

T

L

I

R

R

T

T

A

A

L

L

A

A

M

M

G

G

A

A

D

D

R

R

A

G

I

I

L

H

I

V

-

E

-

V

-

P

V

A

A

A

E

E

-

A

D

N

K

H

V

L

E

G

P

P

L

L

G

Q

V

V

A

A

K

R

L

V

L

L

A

A

K

K

V

L

A

A

E

E

A

K

E

E

A

K

P

V

G

D

L

L

I

V

I

L

L

L

G

G

K

K

Q

Q

A

A

I

I

D

D

N

C

N

N

Q

Q

T

T

G

G

Q

Q

M

M

L

T

G

A

A

G

L

F

L

L

G

D

W

W

G

P

Q

Q

G

G

T

T

F

F

A

A

N

S

K

Q

V

V

E

T

I

L

A

E

G

G

E

D

Q

K

V

I

D

K

V

V

T

E

R

R

E

E

V

I

D

D

G

G

L

L

E

E

T

T

V

L

K

R

L

L

K

K

L

L

P

P

A

A

I

V

V

V

T

T

T

A

D

D

L

L

R

R

L

L

N

N

E

E

P

P

R

R

Y

Y

A

A

S

T

L

L

P

P

N

N

I

I

M

M

K
|
K

A

A

K

K

S
x
K

K

K

P

K

L

I

D

E

Q

V

K

I

T

K

P

A

A

G

D

D

Y

L

G

G

V

V

D

D

V

L

A

T

P

S

R

K

L

L

T

S

V

V

V

I

S

S

V

V

T

E

E

D

P

P

A

P

K

Q

R

R

Q

T

A

A

G

G

V

V

K

K

V

V

A

E

D

T

V

T

D

E

E

D

L

L

V

V

G

A

K

K

L

L

K

K

A

E

L

I

G

G

K200 (= K193) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
K203 (≠ S196) modified: N6,N6,N6-trimethyllysine; by ETFBKMT

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

P53570 Electron transfer flavoprotein subunit beta; Beta-ETF; Electron transfer flavoprotein small subunit; ETFSS from Methylophilus methylotrophus (Bacterium W3A1) (see paper)
33% identity, 84% coverage: 1:209/249 of query aligns to 1:215/264 of P53570

query
sites
P53570

M

M

K

K

I

I

L

L

V

V

P
x
A

V

V

K

K

R

Q

V

T

L

A

D

A

Y

L

N

E

V

E

K

D

P

F

R

E

V

I

K

R

A

E

D

D

G

G

T

M

G

D

V

V

D

D

L

E

A

D

N

F

V

M

K

M

M

Y

S

D

M

L

N
|
N

P
x
E

F
x
W

D
|
D

E

D

I

F

A

S

V

L

E

E

A

A

I

M

R

K

L

I

K

K

E

E

K

S

G

S

A

D

A

T

-

D

T

V

E

E

I

V

V

V

S

S

I

V

G

G

E

P

Q

D

K

R

A

V

Q

D

E

E

T

S

L

L

R

R

T

K

A

C

L

L

A

A

M

K

G

G

A

A

D

D

R

R

A

A

I

V

L

R

I

V

V

W

-

D

A

A

E

A

D

E

K

G

V

S

E

D

P

A

L

I

G

V

V

V

A

G

K

R

L

I

L

L

A

T

K

E

V

V

A

I

E

K

A

K

E

E

A

A

P

P

G

D

L

M

I

V

I

F

L

A

G
|
G

K
x
V

Q
|
Q

A
x
S

I

S

D

D

D

Q

D

A

N
x
Y

N
x
A

Q
x
S

T
|
T

G

G

Q

I

M

S

L

V

G

A

A

S

L

Y

L

L

G

N

W

W

G

P

Q

H

G

A

T

A

F

V

A

V

N

A

K

D

V

L

E

Q

I

Y

-

K

-

P

A

G

G

D

E

N

Q

K

V

A

D

V

V

I

T

R

R

R

E

E

V

L

D

E

G

G

-

M

L

L

E

Q

T

E

V

V

K

E

L

I

K

N

L

C

P

P

A

A

I

V

V

L

T

T

T

I

D

Q

L

L

R

G

L

I

N

N

E

K

P

P

R

R

Y

Y

A

A

S

S

L

L

P

R

N

G

I

I

M

K

K

Q

A

A

K

A

S

T

K

K

P

P

L

I

D

E

Q

E

K

V

T

S

P

L

A

A

D

D

Y

I

G

G

V

L

A6 (≠ P6) binding
NEWD 36:39 (≠ NPFD 36:39) binding
GVQS 119:122 (≠ GKQA 116:119) binding
YAST 127:130 (≠ NNQT 124:127) binding

6fahB Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
33% identity, 98% coverage: 1:243/249 of query aligns to 1:257/262 of 6fahB

query
sites
6fahB

M

M

K

R

I

I

L

L

V

V

P
x
C

V
x
A

K
|
K

R

Q

V

V

L

P

D

D

Y

T

N

N

V

-

K

-

P

-

R

E

V

V

K

K

A

I

D

D

-

P

G

K

T

T

G

G

V

T

D

M

L

I

-

R

A

E

N

G

V

V

K

P

M

S

S

I

M

L

N

N

P

P

F

D

D
|
D

E

A

I

N

A

A

V

L

E

E

E

A

A

A

I

L

R

V

L

I

K

K

E

D

K

E

G

N

A

P

A

G

T

T

E

E

I

V

V

I

V

V

V

M

S
x
T

I
x
M

G

G

E

P

Q

P

K

Q

A

A

Q

S

E

E

T

M

L

L

R

R

T

E

A

C

L

L

A

A

M

M

G

G

A

A

D

D

R

E

A

A

I

Y

L

L

I

L

V

-

A

-

E

S

D

D

K

R

V

A

E

-

P

-

L

F

G

G

-

G

-
x
A

-
x
D

-
x
T

-

W

-

A

-
x
T

V

S

A

A

K

T

L
|
L

L

A

A

A

K

G

V

I

A

K

E

K

A

V

E

K

A

K

P

V

G

D

L

L

I

V

I

L

L
x
A

G
|
G

K

R

Q
|
Q

A
|
A

I

I

D

D

D
x
G

D

D

N
x
T

N
x
A

Q
|
Q

T
x
V

G

G

Q

S

M

Q

L

I

G

A

A

Q

L

R

L

L

G

K

W

M

G

P

Q

V

G

V

T

T

F

Y

A

V

N

E

K

D

V

I

E

K

I

I

A

E

G

D

E

K

Q

K

V

A

D

I

V

V

T

H

R

R

E

Q

V

M

D

E

G

D

G

G

L

Y

E

E

T

V

V

I

K

E

L

V

K

Q

L

L

P

P

A

C

I

L

V

L

T

T

T

C

D

V

L

K

R

E

L

L

N

N

E

D

P

P

R

R

Y

Y

A

M

S

S

L

V

P

G

N

G

I

I

M

M

K

D

A

A

K

Y

S

E

K

Q

P

P

L

I

D

T

-

I

-

W

-

N

-

H

-

E

-

D

-

I

Q

G

K

L

T

S

P

P

A

E

D

A

Y

C

G

G

V

L

D

N

V

A

A

S

P

P

R
x
T

L

Q

T
x
V

V

F

V

R

S

S

V

F

T

S

E

P

P

P

A

A

K

K

-

G

-

E

R

M

Q

I

A

T

G

G

V

T

K

T

V

V

A

N

D

E

V

V

-

A

D

G

E

S

L

L

V

V

G

S

K

K

L

L

K

K

binding flavin-adenine dinucleotide: C6 (≠ P6), A7 (≠ V7), K8 (= K8), D38 (= D39), T61 (≠ S62), M62 (≠ I63), A93 (vs. gap), D94 (vs. gap), T95 (vs. gap), T98 (vs. gap), L102 (= L100), A117 (≠ L115), G118 (= G116), Q120 (= Q118), A121 (= A119), G124 (≠ D122), T126 (≠ N124), A127 (≠ N125), Q128 (= Q126), V129 (≠ T127), T223 (≠ R214), V225 (≠ T216)

1o96A Structure of electron transferring flavoprotein for methylophilus methylotrophus. (see paper)
33% identity, 84% coverage: 1:209/249 of query aligns to 1:214/261 of 1o96A

query
sites
1o96A

M

M

K

K

I

I

L

L

V

V

P
x
A

V
|
V

K
|
K

R

Q

V

T

L

A

-

A

-

L

-

E

D

D

Y

F

N

E

V

I

K

R

P

-

R

-

V

-

K

-

A

-

D

D

G

G

T

M

G

D

V

V

D

D

L

E

A

D

N

F

V

M

K

M

M

Y

S

D

M

L

N
|
N

P

E

F
x
W

D
|
D

E

D

I

F

A

S

V

L

E

E

A

A

I

M

R

K

L

I

K

K

E

E

K

S

G

S

A

D

A

T

-

D

T

V

E

E

I

V

V

V

S
|
S

I
x
V

G

G

E

P

Q

D

K

R

A

V

Q

D

E

E

T

S

L

L

R

R

T

K

A

C

L

L

A

A

M

K

G

G

A

A

D

D

R

R

A

A

I

V

L

R

I

V

V

W

-

D

A

A

E

A

D

E

K

G

V

S

E

D

P

A

L

I

G

V

V
|
V

A

G

K

R

L

I

L
|
L

A

T

K

E

V

V

A

I

E

K

A

K

E

E

A

A

P

P

G

D

L

M

I

V

I

F

L
x
A

G
|
G

K
x
V

Q
|
Q

A
x
S

I

S

D

D

D

Q

D
x
A

N
x
Y

N
x
A

Q
x
S

T
|
T

G

G

Q

I

M

S

L

V

G

A

A

S

L

Y

L

L

G

N

W

W

G

P

Q

H

G

A

T

A

F

V

A

V

N

A

K

D

V

L

E

Q

I

Y

-

K

-

P

A

G

G

D

E

N

Q

K

V

A

D

V

V

I

T

R

R

R

E

E

V

L

D

E

G

G

-

M

L

L

E

Q

T

E

V

V

K

E

L

I

K

N

L

C

P

P

A

A

I

V

V

L

T

T

T

I

D
x
Q

L

L

R

G

L

I

N

N

E

K

P

P

R

R

Y

Y

A

A

S

S

L

L

P

R

N

G

I

I

M

K

K

Q

A

A

K

A

S

T

K

K

P

P

L

I

D

E

Q

E

K

V

T

S

P

L

A

A

D

D

Y

I

G

G

V

L

binding adenosine monophosphate: A6 (≠ P6), V7 (= V7), K8 (= K8), N35 (= N36), D38 (= D39), S62 (= S62), V63 (≠ I63), V99 (= V97), L103 (= L101), A117 (≠ L115), G118 (= G116), V119 (≠ K117), Q120 (= Q118), S121 (≠ A119), A125 (≠ D123), Y126 (≠ N124), A127 (≠ N125), S128 (≠ Q126), T129 (= T127)
binding flavin-adenine dinucleotide: W37 (≠ F38), Q120 (= Q118), Q182 (≠ D177)

7koeA Electron bifurcating flavoprotein fix/etfabcx (see paper)
34% identity, 78% coverage: 35:229/249 of query aligns to 35:244/282 of 7koeA

query
sites
7koeA

M

I

N
|
N

P

P

F

D

D
|
D

E

E

I

R

A

A

V

L

E

E

E

L

A

A

I

S

R

Q

L

L

K

K

E

E

K

K

G

F

A

G

A

A

T

T

E

-

I

V

V

Y

V

V

V

I

S
x
T

I
x
M

G

G

E

P

Q

P

K

Q

A

A

Q

K

E

E

T

A

L

L

R

K

T

D

A

A

L

I

A

A

M

F

G

G

A

L

D

D

R

E

A

A

I

V

L

H

I

L

-

S

-

D

-

R

-

T

-

F

-

A

-

G

-
x
A

-
x
D

-
x
T

V

L

A

A

E
x
T

D

T

K

Y

V

T

E
x
L

P

Y

L

W

G

G

V

I

A

K

K

K

L

I

L

E

A

E

K

R

V

I

A

G

E

K

A

I

E

D

A

-

P

-

G

-

L

L

I

I

I

L

L
x
T

G
|
G

K

K

Q
|
Q

A
|
A

I

V

D
|
D

D
x
G

D

D

N
x
T

N
x
G

Q
|
Q

T
x
V

G

G

Q

P

M

G

L

L

G

A

A

T

L

R

L

F

G

G

W

Y

G

A

Q

L

G

G

T

A

F

Y

A

V

N

V

K

R

V

I

E

E

-

E

I

I

A

D

G

P

E

E

Q

K

V

K

D

E

-

M

-

V

V

I

T

V

R

R

E

R

V

L

D

D

G

Q

G

G

L

F

E

E

T

K

V

I

K

R

L

L

K

K

L

L

P

P

A

A

I

V

V

L

T

T

T

I

D

T

L

D

R

E

L

L

N

N

E

K

P

P

R

R

Y

Y

A

A

S

D

L

L

P

P

N

N

I

L

M

I

K

R

A

A

-

I

K

R

S

Y

K

E

P

P

L

I

D

V

Q

W

K

-

T

T

P

H

A

K

D

D

Y

L

G

G

V

L

D

D

-

P

-

K

-

C

-

G

-

F

V

F

A

G

P

S

R

P

L

T

T

R

V
|
V

V

V

S

S

V

T

T

N

E

I

P

P

A

P

K

A

R

R

Q

K

A

G

G

G

binding flavin-adenine dinucleotide: N36 (= N36), D39 (= D39), T61 (≠ S62), M62 (≠ I63), A93 (vs. gap), D94 (vs. gap), T95 (vs. gap), T98 (≠ E89), L102 (≠ E93), T121 (≠ L115), G122 (= G116), Q124 (= Q118), A125 (= A119), D127 (= D121), G128 (≠ D122), T130 (≠ N124), G131 (≠ N125), Q132 (= Q126), V133 (≠ T127), V232 (= V217)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 7, 8, 9

5ol2B The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
35% identity, 78% coverage: 1:194/249 of query aligns to 1:194/260 of 5ol2B

query
sites
5ol2B

M

M

K

N

I

I

L

V

V

V

P
x
C

V
x
I

K
|
K

R

Q

V

V

L

P

D

D

Y

T

N

T

V

-

K

-

P

-

R

E

V

V

K

K

A

L

D

D

-

P

G

N

T

T

G

G

V

T

D

L

L

I

A

R

N

D

-

G

V

V

K

P

M

S

S

I

M

I

N

N

P

P

F

D

D
|
D

E

K

I

A

A

G

V

L

E

E

A

A

I

I

R

K

L

L

K

K

E

E

K

E

G

M

A

G

A

A

T

-

E

H

I

V

V

T

V

V

V

I

S
x
T

I
x
M

G

G

E

P

Q

P

K

Q

A

A

Q

D

E

M

T

A

L

L

R

K

T

E

A

A

L

L

A

A

M

M

G

G

A

A

D

D

R

R

A

G

I

I

L

L

I

L

V

T

-

D

-

R

A

A

E

F

D

A

K

G

V
x
A

E
x
D

P
x
T

L

W

G

A

V
x
T

A

S

K

S

L

A

L
|
L

A

A

K

G

V

A

A

L

E

K

A

N

E

I

A

D

P

F

G

D

L

I

I

I

L
x
A

G
|
G

K
x
R

Q
|
Q

A
|
A

I

I

D

D

D
x
G

D
|
D

N
x
T

N
x
A

Q
|
Q

T
x
V

G
|
G

Q

P

M

Q

L

I

G

A

A

E

L

H

L

L

G

N

W

L

G

P

Q

S

G

I

T

T

F

Y

A

A

N

E

K

E

V

I

E

K

I

T

A

E

G

G

E

E

Q

Y

V

V

D

L

V

V

T

K

R

R

E

Q

V

F

D

E

G

D

G

C

L

C

E

H

T

D

V

L

K

K

L

V

K

K

L

M

P

P

A

C

I

L

V

I

T

T

D

L

L

K

R

D

L

M

N

N

E

T

P

P

R

R

Y

Y

A

M

S

K

L

V

P

G

N

R

I

I

M

Y

K

D

A

A

binding flavin-adenine dinucleotide: C6 (≠ P6), I7 (≠ V7), K8 (= K8), D38 (= D39), T60 (≠ S62), M61 (≠ I63), A92 (≠ V92), D93 (≠ E93), T94 (≠ P94), T97 (≠ V97), L101 (= L101), A115 (≠ L115), G116 (= G116), R117 (≠ K117), Q118 (= Q118), A119 (= A119), G122 (≠ D122), D123 (= D123), T124 (≠ N124), A125 (≠ N125), Q126 (= Q126), V127 (≠ T127), G128 (= G128)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 222

4l2iB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
35% identity, 78% coverage: 1:195/249 of query aligns to 1:195/263 of 4l2iB

query
sites
4l2iB

M

M

K

N

I

I

L

V

V

V

P
x
C

V
|
V

K
|
K

R

Q

V

V

L

P

D

D

Y

-

N

-

V

-

K

T

P

A

R

E

V

M

K

K

A

I

D

D

G

P

T

V

G

T

V

N

D

N

L

L

A

V

N

R

-

D

-

G

V

V

K

T

M

N

S

I

M

M

N

N

P
|
P

F
x
Y

D
|
D

E

Q

I
x
Y

A

A

V

L

E

E

E

T

A

A

I

L

R

Q

L

L

K

K

E

D

K

E

G

L

A

G

A

A

T

-

E

H

I

V

V

T

V

V

V

I

S
x
T

I
x
M

G
|
G

E
x
P

Q

P

K

H

A

A

Q

E

E

S

T

V

L

L

R

R

T

D

A

C

L

L

A

A

M

V

G

G

A

A

D

D

R

E

A

A

I

K

L

L

I
x
V

V
x
S

-
x
D

-
x
R

A

A

E
x
F

D
x
G

K
x
G

V
x
A

E
x
D

P
x
T

L

L

G

A

V
x
T

A

S

K

A

L

A

L
x
M

A

A

K

N

-

T

V

I

A

K

E

H

A

F

E

G

A

V

P

P

G

D

L

L

I

I

I

L

L
x
C

G
|
G

K

R

Q
|
Q

A
|
A

I

I

D

D

D
x
G

D
|
D

N
x
T

N
x
A

Q
|
Q

T
x
V

G

G

Q

P

M

E

L

I

G

A

A

E

L

H

L

L

G

G

W

L

G

P

Q

Q

G

V

T

T

F

A

A

A

N

L

K

K

V

V

E

Q

I

V

A

K

G

D

E

D

Q

T

V

V

D

V

V

V

T

D

R

R

E

D

V

N

D

E

G

Q

G

M

L

S

E

M

T

T

V

F

K

T

L

M

K

K

L

M

P

P

A

C

I

V

V

V

T

T

T

V

D

-

L

M

R

R

L

S

N

K

E

D

P

L

R

R

Y

F

A

A

S

S

L

I

P

R

N

G

I

K

M

M

K

K

A

A

K

R

binding flavin-adenine dinucleotide: C6 (≠ P6), V7 (= V7), K8 (= K8), P36 (= P37), Y37 (≠ F38), D38 (= D39), Y40 (≠ I41), T60 (≠ S62), M61 (≠ I63), A92 (≠ V92), D93 (≠ E93), T94 (≠ P94), T97 (≠ V97), M101 (≠ L101), C116 (≠ L115), G117 (= G116), Q119 (= Q118), A120 (= A119), G123 (≠ D122), D124 (= D123), T125 (≠ N124), A126 (≠ N125), Q127 (= Q126), V128 (≠ T127)
binding nicotinamide-adenine-dinucleotide: M61 (≠ I63), G62 (= G64), P63 (≠ E65), V84 (≠ I86), S85 (≠ V87), D86 (vs. gap), R87 (vs. gap), F89 (≠ E89), G90 (≠ D90), G91 (≠ K91), A92 (≠ V92)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 221, 223

4kpuB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
35% identity, 78% coverage: 1:195/249 of query aligns to 1:195/263 of 4kpuB

query
sites
4kpuB

M

M

K

N

I

I

L

V

V

V

P
x
C

V
|
V

K
|
K

R

Q

V

V

L

P

D

D

Y

-

N

-

V

-

K

T

P

A

R

E

V

M

K

K

A

I

D

D

G

P

T

V

G

T

V

N

D

N

L

L

A

V

N

R

-

D

-

G

V

V

K

T

M

N

S

I

M

M

N

N

P

P

F
x
Y

D
|
D

E

Q

I
x
Y

A

A

V

L

E

E

E

T

A

A

I

L

R

Q

L

L

K

K

E

D

K

E

G

L

A

G

A

A

T

-

E

H

I

V

V

T

V

V

V

I

S
x
T

I
x
M

G

G

E

P

Q

P

K

H

A

A

Q

E

E

S

T

V

L

L

R

R

T

D

A

C

L

L

A

A

M

V

G

G

A

A

D

D

R

E

A

A

I

K

L

L

I

V

V

S

-

D

-

R

A

A

E

F

D

G

K

G

V
x
A

E
x
D

P
x
T

L

L

G

A

V
x
T

A

S

K

A

L

A

L
x
M

A

A

K

N

-

T

V

I

A

K

E

H

A

F

E

G

A

V

P

P

G

D

L

L

I

I

I

L

L
x
C

G
|
G

K

R

Q
|
Q

A
|
A

I

I

D

D

D
x
G

D
|
D

N
x
T

N
x
A

Q
|
Q

T
x
V

G

G

Q

P

M

E

L

I

G

A

A

E

L

H

L

L

G

G

W

L

G

P

Q

Q

G

V

T

T

F

A

A

A

N

L

K

K

V

V

E

Q

I

V

A

K

G

D

E

D

Q

T

V

V

D

V

V

V

T

D

R

R

E

D

V

N

D

E

G

Q

G

M

L

S

E

M

T

T

V

F

K

T

L

M

K

K

L

M

P

P

A

C

I

V

V

V

T

T

T

V

D

-

L

M

R

R

L

S

N

K

E

D

P

L

R

R

Y

F

A

A

S

S

L

I

P

R

N

G

I

K

M

M

K

K

A

A

K

R

binding flavin-adenine dinucleotide: C6 (≠ P6), V7 (= V7), K8 (= K8), Y37 (≠ F38), D38 (= D39), Y40 (≠ I41), T60 (≠ S62), M61 (≠ I63), A92 (≠ V92), D93 (≠ E93), T94 (≠ P94), T97 (≠ V97), M101 (≠ L101), C116 (≠ L115), G117 (= G116), Q119 (= Q118), A120 (= A119), G123 (≠ D122), D124 (= D123), T125 (≠ N124), A126 (≠ N125), Q127 (= Q126), V128 (≠ T127)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 221, 223

7qh2B Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
30% identity, 79% coverage: 2:198/249 of query aligns to 3:201/265 of 7qh2B

query
sites
7qh2B

K

K

I

I

L

L

V

V

P
x
C

V
x
I

K
|
K

R

Q

V

V

-

P

-

G

-

T

L

S

D

N

Y

V

N

E

V

V

K

D

P

P

R

E

V

-

K

-

A

-

D

-

G

-

T

T

G

G

V

V

D

L

L

I

A

R

N

D

-

G

V

V

K

E

M

S

S

K

M

L

N

N

P

P

F

Y

D
|
D

E

L

I

F

A

G

V

L

E

E

E

T

A

A

I

F

R

R

L

L

K

K

E

E

K

Q

G

L

A

G

T

T

E

-

I

I

V

T

V

L

S
|
S

I
x
M

G

G

E

P

Q

M

K

Q

A

S

Q

K

E

E

T

V

L

L

R

M

T

E

A

S

L

F

A

Y

M

M

G

G

A

A

D

D

R

E

A

G

I

C

L

L

I

L

V

-

A

-

E

S

D

D

K

R

V

-

E

-

P

K

L

F

G

G

V

G

A
|
A

K
x
D

L
x
V

L

V

A

A

K

-

V
x
T

A

S

E

Y

A

T

E
x
L

A

A

P

Q

G

G

-

T

-

K

-

R

-

L

-

G

-

D

-

F

-

D

L

L

I

I

L
x
C

G
|
G

K
|
K

Q
|
Q

A
x
T

I

T

D

D

D
x
G

D
|
D

N
x
T

N
x
A

Q
|
Q

T
x
V

G

G

Q

P

M

E

L

M

G

A

A

E

L

F

L

L

G

G

W

I

G

P

Q

H

G

V

T

T

F

N

A

V

N

I

K

K

V

I

E

L

I

A

A

A

G

D

E

E

Q

K

-

G

V

L

D

T

V

L

T

Q

R

M

E

N

V

M

D

E

G

E

G

S

L

L

E

E

T

I

V

Q

K

R

L

V

K

P

L

Y

P

P

A

C

I

L

V

I

T

T

T

V

D

D

L

K

R

D

L

I

N

Y

E

T

P

P

R

R

Y

L

A

P

S

S

L

Y

P

K

N

R

I

K

M

L

K

D

A

I

K

S

S

K

K

N

P

P

binding flavin-adenine dinucleotide: C7 (≠ P6), I8 (≠ V7), K9 (= K8), D39 (= D39), S61 (= S62), M62 (≠ I63), A93 (= A98), D94 (≠ K99), V95 (≠ L100), T98 (≠ V104), L102 (≠ E108), C117 (≠ L115), G118 (= G116), K119 (= K117), Q120 (= Q118), T121 (≠ A119), G124 (≠ D122), D125 (= D123), T126 (≠ N124), A127 (≠ N125), Q128 (= Q126), V129 (≠ T127)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 226

5ow0B Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
31% identity, 81% coverage: 1:201/249 of query aligns to 1:201/251 of 5ow0B

query
sites
5ow0B

M

M

K

Q

I

I

L

V

V

V

P
x
L

V
x
A

K
|
K

R

V

V

V

L

P

D

D

Y
|
Y

N

E

V

V

K

-

P

P

R

S

V

A

K

D

A

F

D

E

G

L

T

V

G

G

V

-

D

N

L

R

A

A

N

H

V

P

K

R

M

Y

S

T

-

R

-

M

M

I

N

G

P
x
L

F
x
Y

D
|
D

E

E

I

N

A

A

V

V

E

E

E

L

A

G

I

V

R

Q

L

L

K

K

E

E

K

K

G

L

A

G

A

A

T

-

E

D

I

L

V

T

V

V

S
|
S

I
x
Y

G

G

E

R

Q

N

K

D

A

D

Q

V

E

Q

T

F

L

L

R

R

T

K

A

A

L

L

A

A

M

M

G

G

A

A

D

D

R

K

A

V

I

V

L

L

I

-

V

V

A

E

E

G

D

D

K

S

V

D

E

D

P

P

L

Y

G

V

V
x
I

A

A

K

A

L

N

L
|
L

A

K

K

D

V

A

A

I

E

D

A

R

E

Q

A

G

P

T

-

V

G

D

L

L

I

I

I

L

L
x
A

G
|
G

K

R

Q
|
Q

A
x
S

I
x
S

D

D

D

M

D

D

N
x
R

N
x
G

Q
x
V

T
x
V

G
x
P

Q

G

M

V

L

L

G

A

A

G

L

M

L

L

G

D

W

L

G

P

Q

F

G

V

T

P

F

Q

A

A

N

C

K

S

V

V

E

E

I

S

A

V

G

D

E

G

Q

G

V

W

D

K

V

I

T

S

R

Q

E

I

V

T

D

E

G

T

G

G

L

K

E

R

T

L

V

L

K

K

L

L

K

S

L

G

P

K

A

G

I

V

V

L

T

S

-

I

T

T

D

S

L

V

R

P

L

E

N

N

E

V

P

P

R

R

Y

I

A

P

S

A

L

V

P

K

N

A

I

I

M

F

K

A

A

A

K

K

S

K

K

K

P

P

L

V

D

E

Q

K

binding adenosine monophosphate: L6 (≠ P6), A7 (≠ V7), K8 (= K8), D39 (= D39), S61 (= S62), Y62 (≠ I63), I95 (≠ V97), L99 (= L101), A114 (≠ L115), G115 (= G116), Q117 (= Q118), S118 (≠ A119), R123 (≠ N124), G124 (≠ N125), V125 (≠ Q126), V126 (≠ T127), P127 (≠ G128)
binding flavin-adenine dinucleotide: Y13 (= Y13), L37 (≠ P37), Y38 (≠ F38), Q117 (= Q118), S119 (≠ I120)

Query Sequence

>Ga0059261_2886 FitnessBrowser__Korea:Ga0059261_2886
MKILVPVKRVLDYNVKPRVKADGTGVDLANVKMSMNPFDEIAVEEAIRLKEKGAATEIVV
VSIGEQKAQETLRTALAMGADRAILIVAEDKVEPLGVAKLLAKVAEAEAPGLIILGKQAI
DDDNNQTGQMLGALLGWGQGTFANKVEIAGEQVDVTREVDGGLETVKLKLPAIVTTDLRL
NEPRYASLPNIMKAKSKPLDQKTPADYGVDVAPRLTVVSVTEPAKRQAGVKVADVDELVG
KLKALGVAK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory