SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ga0059261_3109 FitnessBrowser__Korea:Ga0059261_3109 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3mqhA Crystal structure of the 3-n-acetyl transferase wlbb from bordetella petrii in complex with coa and udp-3-amino-2-acetamido-2,3-dideoxy glucuronic acid (see paper)
70% identity, 95% coverage: 1:182/192 of query aligns to 2:183/191 of 3mqhA

query
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3mqhA

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binding coenzyme a: F76 (= F75), T84 (= T83), N85 (= N84), V86 (= V85), Y87 (= Y86), P89 (= P88), G111 (= G110), A112 (= A111), A130 (= A129), N135 (= N134), V145 (≠ L144), P148 (= P147), R150 (= R149), Q161 (≠ R160)
binding (2S,3S,4R,5R,6R)-5-(acetylamino)-4-amino-6-{[(R)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-3-hydroxytetrahydro-2H-pyran-2-carboxylic acid: W24 (= W23), H28 (= H27), F46 (≠ Y45), Q60 (= Q59), N61 (= N60), Y66 (= Y65), F76 (= F75), N85 (= N84), V86 (= V85), R95 (= R94), Y99 (= Y98)

3mqgC Crystal structure of the 3-n-acetyl transferase wlbb from bordetella petrii in complex with acetyl-coa (see paper)
70% identity, 95% coverage: 1:182/192 of query aligns to 3:184/192 of 3mqgC

query
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3mqgC

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binding acetyl coenzyme *a: Y67 (= Y65), F77 (= F75), P80 (= P78), T85 (= T83), N86 (= N84), V87 (= V85), Y88 (= Y86), P90 (= P88), G112 (= G110), A113 (= A111), F128 (= F126), G130 (= G128), A131 (= A129), N136 (= N134), V146 (≠ L144), P149 (= P147), R151 (= R149), Q162 (≠ R160)
binding uridine-5'-monophosphate: H29 (= H27), F47 (≠ Y45)
binding uridine-5'-diphosphate: W25 (= W23), S41 (≠ A39), N62 (= N60)

G3XD01 UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronate N-acetyltransferase; UDP-D-GlcNAc3NA N-acetyltransferase; UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronic acid 3-N-acetyltransferase; EC 2.3.1.201 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
68% identity, 97% coverage: 5:190/192 of query aligns to 6:189/191 of G3XD01

query
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G3XD01

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K136 (≠ T135) mutation to A: Reduction in catalytic activity.; mutation to R: No effect on catalytic activity.

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
41% identity, 83% coverage: 4:163/192 of query aligns to 2:153/294 of 4mzuF

query
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4mzuF

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binding coenzyme a: N81 (= N84), D82 (≠ V85), K83 (≠ Y86), G100 (= G110), A101 (= A111), L106 (≠ V116), G118 (= G128), A119 (= A129), T124 (≠ N134), K125 (≠ T135), I134 (≠ L144), N136 (≠ V146), R139 (= R149)
binding thymine: W20 (= W23), Q21 (≠ H24), C38 (≠ G41)

Sites not aligning to the query:

binding thymidine-5'-diphosphate: 189, 203, 205, 270

7s42A Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-acetamido-3,6-dideoxy-d- galactose (see paper)
46% identity, 71% coverage: 4:139/192 of query aligns to 4:137/145 of 7s42A

query
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7s42A

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binding [(2~{R},3~{R},4~{S},5~{R},6~{R})-4-acetamido-6-methyl-3,5-bis(oxidanyl)oxan-2-yl] [[(2~{R},3~{S},5~{R})-5-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: F44 (≠ Y45), E46 (≠ G47), W64 (≠ Y65), N83 (= N84), D84 (≠ V85), Y92 (≠ T93)
binding coenzyme a: C82 (≠ T83), D84 (≠ V85), K85 (≠ Y86), Y86 (≠ N87), P87 (= P88), L114 (≠ V116), T132 (≠ N134), K133 (≠ T135)

7s41A Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-acetamido-3,6-dideoxy-d- glucose (see paper)
46% identity, 71% coverage: 4:139/192 of query aligns to 4:137/145 of 7s41A

query
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7s41A

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binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-4-acetamido-6-methyl-3,5-bis(oxidanyl)oxan-2-yl] [[(2~{R},3~{S},5~{R})-5-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate : F44 (≠ Y45), E46 (≠ G47), W64 (≠ Y65), N83 (= N84), D84 (≠ V85), Y92 (≠ T93)
binding coenzyme a: C82 (≠ T83), D84 (≠ V85), K85 (≠ Y86), Y86 (≠ N87), P87 (= P88), L114 (≠ V116), T132 (≠ N134), K133 (≠ T135)

7s3wA Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-amino-3,6-dideoxy-d- galactose (see paper)
46% identity, 71% coverage: 4:139/192 of query aligns to 4:137/145 of 7s3wA

query
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7s3wA

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W

W

H

Q

W

F

V

C

H

V

V

V

S

L

P

P

G

S

A

A

R

I

I

I

G

G

A

E

G

N

C

C

A

N

L

I

G

C

Q

S

N

H

V

C

Y
x
F

V

I

G
x
E

N

N

D

D

V

V

T

K

I

I

G

G

N

N

V

V

R

T

I

I

Q

K

N

C

N

G

V

V

S

Q

V

I

Y

W

D

D

A

G

V

I

T

E

L

I

E

E

D

D

V

V

F

F

C

I

G

G

P

P

S

N

M

V

V

T

F

F

T
x
C

N

N

V
x
D

Y
x
K

N
x
Y

P
|
P

R

R

S

S

A

K

V

Q

T
x
Y

R

P

K

K

D

-

E

E

Y

F

R

S

R

K

T

T

L

I

I

I

R

K

K

K

G

G

A

A

T

S

L

I

G

G

A

A

N

N

S

A

T

T

I

I

V
x
L

C

P

G

G

V

I

V

T

V

I

G

G

E

E

S

N

A

A

F

M

V

I

G

G

A

A

G

G

A

A

V

I

V

V

N
x
T

T
x
K

D

D

V

V

K

L

P

P

binding oxidized coenzyme a: C82 (≠ T83), D84 (≠ V85), K85 (≠ Y86), Y86 (≠ N87), P87 (= P88), L114 (≠ V116), T132 (≠ N134), K133 (≠ T135)
binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: F44 (≠ Y45), E46 (≠ G47), D84 (≠ V85), Y92 (≠ T93)

7s3uA Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-amino-3,6-dideoxy-d-glucose (see paper)
46% identity, 71% coverage: 4:139/192 of query aligns to 4:137/145 of 7s3uA

query
sites
7s3uA

I

I

H

H

P

K

T

L

A

A

I

D

V

V

D

-

E

Q

G

S

A

K

Q

N

I

I

G

G

E

S

G

G

T

T

R

R

V

I

W

W

H

Q

W

F

V

C

H

V

V

V

S

L

P

P

G

S

A

A

R

I

I

I

G

G

A

E

G

N

C

C

A

N

L

I

G

C

Q

S

N

H

V

C

Y
x
F

V

I

G
x
E

N

N

D

D

V

V

T

K

I

I

G

G

N

N

V

V

R

T

I

I

Q

K

N

C

N

G

V

V

S

Q

V

I

Y

W

D

D

A

G

V

I

T

E

L

I

E

E

D

D

V

V

F

F

C

I

G

G

P

P

S

N

M

V

V

T

F

F

T
x
C

N
|
N

V
x
D

Y
x
K

N
x
Y

P
|
P

R

R

S

S

A

K

V

Q

T
x
Y

R

P

K

K

D

-

E

E

Y

F

R

S

R

K

T

T

L

I

I

I

R

K

K

K

G

G

A

A

T

S

L

I

G

G

A

A

N

N

S

A

T

T

I

I

V
x
L

C

P

G

G

V

I

V

T

V

I

G

G

E

E

S

N

A

A

F

M

V

I

G

G

A

A

G

G

A

A

V

I

V

V

N
x
T

T
x
K

D

D

V

V

K

L

P

P

binding coenzyme a: C82 (≠ T83), N83 (= N84), D84 (≠ V85), K85 (≠ Y86), Y86 (≠ N87), P87 (= P88), L114 (≠ V116), T132 (≠ N134), K133 (≠ T135)
binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: F44 (≠ Y45), E46 (≠ G47), D84 (≠ V85), Y92 (≠ T93)

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
41% identity, 85% coverage: 4:166/192 of query aligns to 2:154/290 of 4mzuB

query
sites
4mzuB

I

I

H

H

P

K

T

L

A

A

I

D

V

V

D

-

E

Q

G

S

A

Q

Q

N

I

I

G

G

E

D

G

N

T

T

R

K

V

V

W
|
W

H
x
Q

W

F

V

C

H

V

V

I

S

L

P

A

G

G

A

A

R

V

I

I

G

G

A

R

G

N

C

C

A

N

L

I

G
x
C

Q
x
A

N

N

V

S

Y

L

V

I

G

E

N

N

D

D

V

V

T

V

I

I

G

G

N

D

N

N

V

V

R

T

I

I

Q

K

N

S

N
x
G

V

V

S

Q

V

I

Y

W

D

D

A

G

V

I

T

H

L

I

E

Q

D

D

V

V

F
|
F

C

I

G

G

P

P

S
x
N

M

V

V

T

F

F

T
|
T

N
|
N

V
x
D

Y
x
K

N

Q

P
|
P

R

R

S

S

A

L

V

-

T

-

R

-

K

-

D

-

E

-

Y

-

R

-

R

K

T

T

L

I

I

V

R

K

K

K

G

G

A

A

T

S

L

I

G
|
G

A
|
A

N

N

S

S

T

T

I

I

V

L

C

P

G

G

V

I

V

L

V

I

G

G

E

E

S

N

A

A

F

M

V

V

G
|
G

A
|
A

G

G

A

A

V

V

V

I

N
x
T

T
x
K

D

N

V

V

K

P

P

D

F

N

A

A

L

I

M

V

L
x
I

G

G

V

N

P

P

A

G

R
|
R

Q

I

A

T

G

G

W

Y

M

V

S

E

A

A

F

N

G

F

E

E

R

-

I

L

P

K

L

L

P

Q

L

M

S

S

binding coenzyme a: F72 (= F75), T80 (= T83), N81 (= N84), D82 (≠ V85), K83 (≠ Y86), P85 (= P88), G99 (= G110), A100 (= A111), G117 (= G128), A118 (= A129), T123 (≠ N134), K124 (≠ T135), I133 (≠ L144), R138 (= R149)
binding magnesium ion: G58 (≠ N61), N76 (≠ S79)
binding thymine: W20 (= W23), Q21 (≠ H24), C38 (≠ G41), A39 (≠ Q42)

Sites not aligning to the query:

binding coenzyme a: 199
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

7s45A Crystal structure of an n-acetyltransferase, c80t mutant, from helicobacter pullorum in the presence of acetyl coenzyme a and dtdp (see paper)
46% identity, 71% coverage: 4:139/192 of query aligns to 4:133/141 of 7s45A

query
sites
7s45A

I

I

H

H

P

K

T

L

A

A

I

D

V

V

D

-

E

Q

G

S

A

K

Q

N

I

I

G

G

E

S

G

G

T

T

R

R

V

I

W

W

H

Q

W

F

V

C

H

V

V

V

S

L

P

P

G

S

A

A

R

I

I

I

G

G

A

E

G

N

C

C

A

N

L

I

G

C

Q

S

N

H

V

C

Y
x
F

V

I

G
x
E

N

N

D

D

V

V

T

K

I

I

G

G

N

N

V

V

R

T

I

I

Q

K

N

C

N

G

V

V

S

Q

V

I

Y
x
W

D

D

A

G

V

I

T

E

L

I

E

E

D

D

V

V

F

F

C

I

G

G

P

P

S

N

M

V

V

T

F

F

T
|
T

N
|
N

V
x
D

Y
x
K

N
x
Y

P
|
P

R

R

S

S

A

K

V

-

T

-

R

-

K

-

D

-

E

Q

Y

F

R

S

R

K

T

T

L

I

I

I

R

K

K

K

G

G

A

A

T

S

L

I

G

G

A

A

N

N

S

A

T

T

I

I

V
x
L

C

P

G

G

V

I

V

T

V

I

G

G

E

E

S

N

A

A

F

M

V

I

G

G

A

A

G

G

A

A

V

I

V

V

N
x
T

T
x
K

D

D

V

V

K

L

P

P

binding acetyl coenzyme *a: W64 (≠ Y65), T82 (= T83), N83 (= N84), D84 (≠ V85), K85 (≠ Y86), Y86 (≠ N87), P87 (= P88), L110 (≠ V116), T128 (≠ N134), K129 (≠ T135)
binding thymidine-5'-diphosphate: F44 (≠ Y45), E46 (≠ G47)

3fsbA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-quinovose (see paper)
32% identity, 76% coverage: 16:161/192 of query aligns to 89:229/260 of 3fsbA

query
sites
3fsbA

I

I

G

G

E

D

G

N

T

F

R

Q

V

T

W

G

H

H

W

K

V

V

H

T

V

I

S

R

P

E

G

N

A

T

R

K

I

I

G

G

A

N

G

N

C

V

A

K

L

I

G
|
G

Q

T

N

L

V

S

Y

D

V

I

G

Q

N

H

D

H

V

V

T

Y

I

I

G

G

N

N

N

Y

V

V

R

N

I

I

Q
x
H

N

S

N

N

V

V

S

F

V

V

Y

G

D

E

A

K

V

S

T

I

L

I

E

K

D

D

D

F

V

V

F

W

C

L

G
x
F

P

P

S

H

M

V

V

V

F

L

T

T

N

N

V

D

Y

P

N

T

P

P

R

P

S

S

A

-

V

-

T

-

R

-

K

-

D

N

E

E

Y

L

R

L

R

G

T

V

L

T

I

I

R

E

K

L

G

F

A

A

T

V

L

I

G
x
A

A
|
A

N

R

S

S

T

V

I

V

V

L

C

P

G

G

V

I

V

H

V

I

G

N

E

E

S

D

A

A

F

L

V

V

G
|
G

A
|
A

G

G

A

A

V

V

N

T

T

K

D

D

V

V

K

P

P

K

F

E

A

T

L

V

M

V

L
x
V

G

G

V

N

P

P

A

A

R

R

Q

E

A

I

G

C

W

S

M

I

S

R

A

K

F

I

G

K

E

N

R

K

I

I

binding coenzyme a: F150 (≠ G77), A178 (≠ G110), A179 (= A111), G196 (= G128), A197 (= A129), V212 (≠ L144)
binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: G114 (= G41), H132 (≠ Q59), F150 (≠ G77)

Sites not aligning to the query:

binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: 78, 241, 243

3fscA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-fucose (see paper)
32% identity, 76% coverage: 16:161/192 of query aligns to 89:229/259 of 3fscA

query
sites
3fscA

I

I

G

G

E

D

G

N

T

F

R

Q

V

T

W

G

H

H

W

K

V

V

H

T

V

I

S

R

P

E

G

N

A

T

R

K

I

I

G

G

A

N

G

N

C

V

A
x
K

L

I

G
|
G

Q
x
T

N

L

V

S

Y

D

V

I

G

Q

N

H

D

H

V

V

T

Y

I

I

G

G

N

N

N

Y

V

V

R

N

I

I

Q
x
H

N
x
S

N

N

V

V

S

F

V

V

Y

G

D

E

A

K

V

S

T

I

L

I

E

K

D

D

D

F

V

V

F

W

C

L

G
x
F

P

P

S

H

M

V

V

V

F

L

T

T

N

N

V

D

Y

P

N

T

P

P

R

P

S

S

A

-

V

-

T

-

R

-

K

-

D

N

E

E

Y

L

R

L

R

G

T

V

L

T

I

I

R

E

K

L

G

F

A

A

T

V

L

I

G
x
A

A
|
A

N

R

S

S

T

V

I

V

V

L

C

P

G

G

V

I

V

H

V

I

G

N

E

E

S

D

A

A

F

L

V

V

G
|
G

A
|
A

G

G

A

A

V

V

N

T

T

K

D

D

V

V

K

P

P

K

F

E

A

T

L

V

M

V

L
x
V

G

G

V

N

P

P

A

A

R

R

Q

E

A

I

G

C

W

S

M

I

S

R

A

K

F

I

G

K

E

N

R
x
K

I

I

binding coenzyme a: F150 (≠ G77), A178 (≠ G110), A179 (= A111), G196 (= G128), A197 (= A129), V212 (≠ L144), K228 (≠ R160)
binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: K112 (≠ A39), G114 (= G41), T115 (≠ Q42), H132 (≠ Q59), S133 (≠ N60)

Sites not aligning to the query:

binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: 78, 243

3fs8A Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d- glucose n-acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with acetyl-coa (see paper)
32% identity, 76% coverage: 16:161/192 of query aligns to 89:229/259 of 3fs8A

query
sites
3fs8A

I

I

G

G

E

D

G

N

T

F

R

Q

V

T

W

G

H

H

W

K

V

V

H

T

V

I

S
x
R

P

E

G

N

A

T

R

K

I

I

G

G

A

N

G

N

C

V

A

K

L

I

G

G

Q

T

N

L

V

S

Y

D

V

I

G

Q

N

H

D

H

V

V

T

Y

I

I

G

G

N

N

N

Y

V

V

R

N

I

I

Q

H

N

S

N

N

V

V

S

F

V

V

Y

G

D

E

A

K

V

S

T

I

L

I

E

K

D

D

D

F

V

V

F

W

C

L

G
x
F

P
|
P

S

H

M

V

V

V

F

L

T

T

N

N

V

D

Y

P

N

T

P

P

R

P

S

S

A

-

V

-

T

-

R

-

K

-

D

N

E

E

Y

L

R

L

R

G

T

V

L

T

I

I

R

E

K

L

G

F

A

A

T

V

L

I

G
x
A

A
|
A

N

R

S

S

T

V

I

V

V

L

C

P

G

G

V

I

V

H

V

I

G

N

E

E

S

D

A

A

F

L

V

V

G
|
G

A
|
A

G

G

A

A

V

V

N

T

T

K

D

D

V

V

K

P

P

K

F

E

A

T

L

V

M

V

L
x
V

G

G

V

N

P

P

A

A

R

R

Q

E

A

I

G

C

W

S

M

I

S

R

A

K

F

I

G

K

E

N

R
x
K

I

I

binding acetyl coenzyme *a: F150 (≠ G77), P151 (= P78), A178 (≠ G110), A179 (= A111), G196 (= G128), A197 (= A129), V212 (≠ L144), K228 (≠ R160)
binding thymine: R102 (≠ S29)

Sites not aligning to the query:

binding thymine: 84

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
30% identity, 54% coverage: 47:149/192 of query aligns to 72:179/190 of 5u2kA

query
sites
5u2kA

G

G

N

W

D

N

V

V

T

K

I

L

G

G

N

K

N

N

V

V

R

Y

I

V

Q

N

N

T

N

N

V

C

S

Y

V

F

Y
x
M

D

D

A

G

-

G

-

Q

V

I

T

T

L

I

E

G

D

D

D

N

V

V

F

F

C

I

G

G

P

P

S

N

M

C

V

G

F

F

T
x
Y

N

T

V
x
A

Y

T

N
x
H

P

P

R

L

S

N

A

F

V

H

T

H

R

R

K

N

D

E

E

G

Y

F

R

E

R

K

T

A

-

G

-

P

-

I

L

H

I

I

R

G

K

S

G

N

A

T

T

W

L

F

G

G

A

G

N

H

S

V

T

A

I

V

V
x
L

C

P

G

G

V

V

V

T

V

I

G

G

E

E

S

G

A

S

F

V

V

I

G

G

A

A

G

G

A

S

V

V

N
x
T

T
x
K

D

D

V

I

K

P

P

P

F

H

A

S

L

L

M

A

L

V

G

G

V

N

P

P

A

C

R

K

binding coenzyme a: M90 (≠ Y65), Y110 (≠ T83), A112 (≠ V85), H114 (≠ N87), L146 (≠ V116), T164 (≠ N134), K165 (≠ T135)

P9WMN3 Bifunctional protein GlmU; EC 2.7.7.23; EC 2.3.1.157 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 4 papers)
34% identity, 67% coverage: 14:142/192 of query aligns to 329:447/495 of P9WMN3

query
sites
P9WMN3

A

S

Q

S

I

I

G

G

E

D

G

G

T

A

R

A

V

V

W

G

H

P

W

F

V

T

H

Y

V

L

S
x
R

P

P

G

G

A

T

R

A

I

L

G

G

A

A

G

D

C

G

A

K

L

L

G

G

Q

A

N

F

V

V

Y

E

V

V

G
x
K

N

N

D

S

V

-

T

T

I

I

G

G

N

T

N

G

V

T

R

K

I

V

Q

P

N

H

N

L

V

T

S
x
Y

V

V

Y

G

D

D

A

A

V

-

T

D

L

I

E

G

D

E

D

Y

V

S

F
x
N

C

I

G

G

P
x
A

S

S

M

S

V

V

F

F

T

V

N

N

V

-

Y

Y

N

D

P

G

R

T

S

S

A

-

V

-

T

-

R

-

K

-

D

-

E

-

Y

K

R

R

T

T

L

T

I

V

R

G

K

S

G

H

A

V

T

R

L

T

G

G

A
x
S

N

D

S

T

T

M

I

F

V

V

C

A

G

P

V

V

V

T

V

I

G

G

E

D

S

G

A

A

F

Y

V

T

G

G

A
|
A

G

G

A

T

V

V

N

R

T

E

D

D

V

V

K

P

P

P

F

G

A

A

L

L

R344 (≠ S29) binding
K362 (≠ G47) binding ; modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
Y377 (≠ S63) binding
N388 (≠ F75) binding
A391 (≠ P78) binding
S416 (≠ A111) binding
A434 (= A129) binding

Sites not aligning to the query:

12:15 binding
26 binding
83 binding
88:89 binding
112:114 binding
114 binding ; binding
151 binding
166 binding
181 binding
239 binding ; binding ; binding

4k6rA Crystal structure of glmu in complex with atp (see paper)
34% identity, 67% coverage: 14:142/192 of query aligns to 324:442/469 of 4k6rA

query
sites
4k6rA

A

S

Q

S

I

I

G

G

E

D

G

G

T

A

R

A

V

V

W

G

H

P

W

F

V

T

H

Y

V

L

S
x
R

P

P

G

G

A

T

R

A

I

L

G

G

A

A

G

D

C

G

A

K

L

L

G

G

Q

A

N

F

V

V

Y

E

V

V

G
x
K

N

N

D

S

V

-

T

T

I

I

G

G

N

T

N

G

V

T

R

K

I

V

Q

P

N

H

N

L

V

T

S
x
Y

V

V

Y

G

D

D

A

A

V

-

T

D

L

I

E

G

D

E

D

Y

V

S

F

N

C

I

G

G

P

A

S

S

M

S

V

V

F

F

T

V

N
|
N

V

-

Y
|
Y

N

D

P

G

R

T

S

S

A
x
K

V

-

T

-

R

-

K

-

D

-

E

-

Y

-

R

R

T

T

L

T

I

V

R

G

K

S

G

H

A

V

T

R

L

T

G

G

A

S

N

D

S

T

T

M

I

F

V

V

C

A

G

P

V

V

V

T

V

I

G

G

E

D

S

G

A

A

F

Y

V

T

G

G

A

A

G

G

A

T

V

V

N

R

T

E

D

D

V

V

K

P

P

P

F

G

A

A

L

L

binding 2-acetamido-2-deoxy-1-O-phosphono-alpha-D-glucopyranose: R339 (≠ S29), K357 (≠ G47), Y372 (≠ S63), N392 (= N84), Y393 (= Y86), K398 (≠ A91)

Sites not aligning to the query:

active site: 14
binding adenosine-5'-triphosphate: 9, 10, 11, 12, 13, 14, 78, 81, 83
binding 2-acetamido-2-deoxy-1-O-phosphono-alpha-D-glucopyranose: 84, 145, 146, 161, 176, 204, 206, 234
binding magnesium ion: 109, 234

3st8A Crystal structure of glmu from mycobacterium tuberculosis in complex with coenzyme a, glucosamine 1-phosphate and uridine-diphosphate-n- acetylglucosamine
34% identity, 67% coverage: 14:142/192 of query aligns to 324:442/475 of 3st8A

query
sites
3st8A

A

S

Q

S

I

I

G

G

E

D

G

G

T

A

R

A

V

V

W

G

H

P

W

F

V

T

H

Y

V

L

S

R

P

P

G

G

A

T

R

A

I

L

G

G

A

A

G

D

C

G

A

K

L

L

G

G

Q

A

N

F

V

V

Y

E

V

V

G

K

N

N

D

S

V

-

T

T

I

I

G

G

N

T

N

G

V

T

R

K

I

V

Q
x
P

N
x
H

N

L

V

T

S

Y

V

V

Y

G

D

D

A

A

V

-

T

D

L

I

E

G

D

E

D

Y

V

S

F
x
N

C

I

G
|
G

P
x
A

S

S

M

S

V

V

F

F

T

V

N

N

V

-

Y

Y

N

D

P

G

R

T

S

S

A

K

V

-

T

-

R

-

K

-

D

-

E

-

Y

-

R

R

T

T

L

T

I

V

R

G

K

S

G

H

A

V

T

R

L

T

G
|
G

A
x
S

N

D

S

T

T

M

I

F

V

V

C

A

G

P

V

V

V

T

V

I

G

G

E

D

S

G

A

A

F

Y

V

T

G
|
G

A
|
A

G

G

A

T

V

V

N

R

T

E

D

D

V

V

K

P

P

P

F

G

A

A

L

L

binding coenzyme a: G385 (= G77), A386 (≠ P78), G410 (= G110), S411 (≠ A111), G428 (= G128), A429 (= A129)
binding 2-amino-2-deoxy-1-O-phosphono-alpha-D-glucopyranose: P368 (≠ Q59), H369 (≠ N60), N383 (≠ F75)

Sites not aligning to the query:

active site: 14
binding coenzyme a: 444, 446
binding magnesium ion: 109, 234
binding uridine-diphosphate-n-acetylglucosamine: 7, 8, 9, 10, 78, 81, 83, 84, 87, 109, 145, 146, 161, 176, 206, 234

6ge9A Structure of mycobacterium tuberculosis glmu bound to glc-1p and ac- coa (see paper)
34% identity, 67% coverage: 14:142/192 of query aligns to 325:443/475 of 6ge9A

query
sites
6ge9A

A

S

Q

S

I

I

G

G

E

D

G

G

T

A

R

A

V

V

W

G

H

P

W

F

V

T

H

Y

V

L

S

R

P

P

G

G

A

T

R

A

I

L

G

G

A

A

G

D

C

G

A

K

L

L

G

G

Q

A

N

F

V

V

Y

E

V

V

G

K

N

N

D

S

V

-

T

T

I

I

G

G

N

T

N

G

V

T

R

K

I

V

Q
x
P

N
x
H

N

L

V

T

S

Y

V

V

Y

G

D

D

A

A

V

-

T

D

L

I

E

G

D

E

D

Y

V

S

F
x
N

C

I

G
|
G

P

A

S
|
S

M

S

V

V

F

F

T

V

N

N

V

-

Y

Y

N

D

P

G

R

T

S

S

A

K

V

-

T

-

R

-

K

-

D

-

E

-

Y

-

R

R

T

T

L

T

I

V

R

G

K

S

G

H

A

V

T

R

L

T

G
|
G

A
x
S

N
x
D

S

T

T

M

I

F

V

V

C

A

G

P

V

V

V

T

V

I

G

G

E

D

S

G

A

A

F

Y

V

T

G
|
G

A
|
A

G

G

A

T

V

V

N

R

T

E

D

D

V

V

K

P

P

P

F

G

A

A

L

L

binding acetyl coenzyme *a: G386 (= G77), G411 (= G110), S412 (≠ A111), G429 (= G128), A430 (= A129)
binding 1-O-phosphono-alpha-D-glucopyranose: P369 (≠ Q59), H370 (≠ N60), N384 (≠ F75)
binding magnesium ion: S388 (= S79), D413 (≠ N112)

Sites not aligning to the query:

active site: 15
binding acetyl coenzyme *a: 445, 447

3sptA Crystal structure of glmu from mycobacterium tuberculosis in complex with acetyl coenzyme a and uridine-diphosphate-n-acetylglucosamine
34% identity, 67% coverage: 14:142/192 of query aligns to 324:442/474 of 3sptA

query
sites
3sptA

A

S

Q

S

I

I

G

G

E

D

G

G

T

A

R

A

V

V

W

G

H

P

W

F

V

T

H

Y

V

L

S

R

P

P

G

G

A

T

R

A

I

L

G

G

A

A

G

D

C

G

A

K

L

L

G

G

Q

A

N

F

V

V

Y

E

V

V

G

K

N

N

D

S

V

-

T

T

I

I

G

G

N

T

N

G

V

T

R

K

I

V

Q

P

N

H

N

L

V

T

S

Y

V

V

Y

G

D

D

A

A

V

-

T

D

L

I

E

G

D

E

D

Y

V

S

F

N

C

I

G
|
G

P
x
A

S

S

M

S

V

V

F

F

T

V

N

N

V

-

Y

Y

N

D

P

G

R

T

S

S

A

K

V

-

T

-

R

-

K

-

D

-

E

-

Y

-

R

R

T

T

L

T

I

V

R

G

K

S

G

H

A

V

T

R

L

T

G
|
G

A
x
S

N

D

S

T

T

M

I

F

V

V

C

A

G

P

V

V

V

T

V

I

G

G

E

D

S

G

A

A

F

Y

V

T

G
|
G

A
|
A

G

G

A

T

V

V

N

R

T

E

D

D

V

V

K

P

P

P

F

G

A

A

L

L

binding acetyl coenzyme *a: G385 (= G77), A386 (≠ P78), G410 (= G110), S411 (≠ A111), G428 (= G128), A429 (= A129)

Sites not aligning to the query:

active site: 14
binding acetyl coenzyme *a: 444, 446
binding magnesium ion: 109, 234
binding uridine-diphosphate-n-acetylglucosamine: 7, 8, 9, 10, 78, 81, 83, 84, 87, 109, 145, 146, 161, 176, 206, 234

3d8vA Crystal structure of glmu from mycobacterium tuberculosis in complex with uridine-diphosphate-n-acetylglucosamine (see paper)
33% identity, 67% coverage: 14:142/192 of query aligns to 329:446/478 of 3d8vA

query
sites
3d8vA

A

S

Q

S

I

I

G

G

E

D

G

G

T

A

R

A

V

V

W

G

H

P

W

F

V

T

H

Y

V

L

S

R

P

P

G

G

A

T

R

A

I

L

G

G

A

A

G

D

C

G

A

K

L

L

G

G

Q

A

N

F

V

V

Y

E

V

V

G

K

N

N

D

S

V

-

T

T

I

I

G

G

N

T

N

G

V

T

R

K

I

V

Q

P

N

H

N

L

V

T

S

Y

V

V

Y

G

D

D

A

A

V

-

T

D

L

I

E

G

D

E

D

Y

V

S

F

N

C

I

G

G

P

A

S

S

M

S

V

V

F

F

T

V

N

N

V

Y

Y

D

N

T

P

S

R

K

S

-

A

-

V

-

T

-

R

-

K

-

D

-

E

-

Y

-

R

R

T

T

L

T

I

V

R

G

K

S

G

H

A

V

T

R

L

T

G

G

A

S

N

D

S

T

T

M

I

F

V

V

C

A

G

P

V

V

V

T

V

I

G

G

E

D

S

G

A

A

F

Y

V

T

G

G

A

A

G

G

A

T

V

V

N

R

T

E

D

D

V

V

K

P

P

P

F

G

A

A

L

L

Sites not aligning to the query:

active site: 19
binding uridine-diphosphate-n-acetylglucosamine: 12, 14, 15, 83, 86, 88, 89, 92, 112, 150, 151, 166, 181, 211, 239

Query Sequence

>Ga0059261_3109 FitnessBrowser__Korea:Ga0059261_3109
VATIHPTAIVDEGAQIGEGTRVWHWVHVSPGARIGAGCALGQNVYVGNDVTIGNNVRIQN
NVSVYDAVTLEDDVFCGPSMVFTNVYNPRSAVTRKDEYRRTLIRKGATLGANSTIVCGVV
VGESAFVGAGAVVNTDVKPFALMLGVPARQAGWMSAFGERIPLPLSGSGDYRCPHSGTLY
RLEGETLTVVIE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory