SitesBLAST

M1 (= M1) modified: Initiator methionine, Removed
C2 (= C2) mutation C->A,S: Loss of glutamine-dependent activity but no effect on ammonia-dependent asparagine synthetase activity.
H30 (= H26) mutation to A: 4,5-fold decrease in glutamine affinity.
D34 (= D30) mutation D->N,E: Little effect on the kinetic properties.
H81 (≠ F80) mutation to A: 5-fold decrease in glutamine affinity.
A105 (≠ E107) mutation to H: Little effect on the kinetic properties.
E349 (≠ Q371) mutation E->A,Q: Loss of glutamine- and ammonia-dependent synthetase activity, but still exhibits glutaminase activity.; mutation to D: 5-fold increase in affinity for aspartate when assaying both the glutamine- and ammonia-dependent synthetase reactions, and 2-fold decrease in kcat for these reactions. Modifies the product glutamate/asparagine stoichiometry.

P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
25% identity, 68% coverage: 1:426/631 of query aligns to 1:420/557 of P78753

query
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P78753

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A

S391 (≠ R397) modified: Phosphoserine

Sites not aligning to the query:

489 modified: Phosphoserine

P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 7 papers)
28% identity, 60% coverage: 1:380/631 of query aligns to 1:374/561 of P08243

query
sites
P08243

M

M

C
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C

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Y

C2 (= C2) active site, For GATase activity; mutation to A: Loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected.
A6 (= A6) to E: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122975
V210 (≠ T220) to E: in dbSNP:rs1049674
F362 (vs. gap) to V: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122973

Sites not aligning to the query:

550 R → C: in ASNSD; increases level of protein abundance; dbSNP:rs398122974

6gq3A Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
28% identity, 60% coverage: 2:380/631 of query aligns to 1:361/509 of 6gq3A

query
sites
6gq3A

C
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C

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Y
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Y

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N

G
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G

E
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E

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Y

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G

Q

G

G

Y

Y

active site: W4 (≠ V5), L49 (= L49), N74 (= N74), G75 (= G75), T324 (≠ F344), R327 (≠ P347)
binding 5-oxo-l-norleucine: C1 (= C2), R48 (= R48), V51 (≠ I51), V52 (≠ I52), Y73 (= Y73), N74 (= N74), G75 (= G75), E76 (= E76), V95 (≠ S100), D96 (= D101)

6lbpA Structure of the glutamine phosphoribosylpyrophosphate amidotransferase from arabidopsis thaliana (see paper)
34% identity, 22% coverage: 40:179/631 of query aligns to 65:209/460 of 6lbpA

query
sites
6lbpA

G

G

D

D

A

I

A

A

L

I

G

G

Q

H

N

V

R

R

L

Y

S

S

I

T

I

A

D

G

L

S

S

S

C

M

A

L

G

K

H

N

-

V

Q

Q

P

P

M

F

W

V

D

A

H

G

S

Y

R

R

-

F

-

G

R

S

Y

V

V

G

I

V

S

A

Y

H

N
|
N

G
|
G

E

N

T

L

Y

V

N

N

F

Y

A

T

A

K

L

L

R

R

A

A

E

D

L

L

E

E

A

-

R

E

H

N

G

G

A

S

I

I

P

-

W

F

T

N

G

T

S

S

D

D

T

T

E

E

V

V

I

L

E

H

G

L

F

I

A

A

-

I

R

S

E

K

G

A

I

R

P

P

F

F

L

F

-

M

-

R

-

I

-

V

-

D

-

A

-

C

D

E

K

K

L

L

N

Q

G

G

I

A

F

Y

A

S

L

M

A

-

I

V

Y

F

D

V

T

T

A

E

D

D

Q

K

V

L

M

V

H

A

V

V

L

-

R

R

D

D

P

P

L

H

G

G

I

F

K

R

P

P

L

L

V

V

-

M

A

G

T

R

E

R

Q

S

H

N

G

G

G

A

I

V

F

V

F

F

C

A

S

S

E

E

I

T

K

-

G

-

L

-

L

C

T

A

L

L

P

D

A

L

L

I

Q

E

R

A

T

T

L

Y

R

E

R

R

E

E

active site: N102 (= N74), G103 (= G75)

Sites not aligning to the query:

active site: 1, 27, 243, 301, 306, 316, 424
binding iron/sulfur cluster: 237, 239, 383, 385, 434, 436, 437

Q9STG9 Amidophosphoribosyltransferase 2, chloroplastic; AtATase2; AtPURF2; PRPP2; Glutamine phosphoribosylpyrophosphate amidotransferase 2; AtGPRAT2; Protein CHLOROPLAST IMPORT APPARATUS 1; Protein DIFFERENTIAL DEVELOPMENT OF VASCULAR ASSOCIATED CELLS; EC 2.4.2.14 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
34% identity, 22% coverage: 40:179/631 of query aligns to 151:295/561 of Q9STG9

query
sites
Q9STG9

G

G

D

D

A

I

A

A

L

I

G

G

Q

H

N

V

R

R

L

Y

S

S

I

T

I

A

D

G

L

S

S

S

C

M

A

L

G

K

H

N

-

V

Q

Q

P

P

M

F

W

V

D

A

H

G

S

Y

R

R

-

F

-

G

R

S

Y

V

V

G

I

V

S

A

Y
x
H

N

N

G

G

E

N

T

L

Y

V

N

N

F

Y

A

T

A

K

L

L

R

R

A

A

E

D

L

L

E

E

A

-

R

E

H

N

G

G

A

S

I

I

P

-

W

F

T

N

G

T

S

S

D

D

T

T

E

E

V

V

I

L

E

H

G

L

F

I

A

A

-

I

R

S

E

K

G

A

I

R

P

P

F

F

L

F

-

M

-

R

-

I

-

V

-

D

-

A

-

C

D

E

K

K

L

L

N

Q

G

G

I

A

F

Y

A

S

L

M

A

-

I

V

Y

F

D

V

T

T

A

E

D

D

Q

K

V

L

M

V

H

A

V

V

L

-

R

R

D

D

P

P

L

H

G

G

I

F

K
x
R

P
|
P

L

L

V

V

-

M

A

G

T

R

E

R

Q

S

H

N

G

G

G

A

I

V

F

V

F

F

C

A

S

S

E

E

I

T

K

-

G

-

L

-

L

C

T

A

L

L

P

D

A

L

L

I

Q

E

R

A

T

T

L

Y

R

E

R

R

E

E

H187 (≠ Y73) mutation to T: In cia1-2; small plants with white leaves showing an irregular mosaic of green sectors.
R264 (≠ K146) mutation to K: Strong resistance to the bleaching herbicides DAS073 and DAS734.
P265 (= P147) mutation to S: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with F-494.

Sites not aligning to the query:

371 G→S: Low resistance to the bleaching herbicides DAS073 and DAS734.
476 P→S: Resistance to the bleaching herbicides DAS073 and DAS734.
494 Y→F: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with S-265.

Query Sequence

>Ga0059261_3121 FitnessBrowser__Korea:Ga0059261_3121
MCGIVAISAPDARRFSLDATLDVIAHRGPDDRGSHVSDGGDAALGQNRLSIIDLSCAGHQ
PMWDHSRRYVISYNGETYNFAALRAELEARHGAIPWTGSSDTEVVIEGFAREGIPFLDKL
NGIFALAIYDTADQVMHVLRDPLGIKPLVATEQHGGIFFCSEIKGLLTLPALQRTLRRES
LAEQLAFMYVPEPHTLYHEFHKVEPGVCFTYRAGKLLARTPLFAWLNNAQPITGEAEAIE
ALRHAFTAAVDRQVVADVPVSLFLSGGLDSSAVADQAVRSGASIHDAYTIAFSDADRSLD
AQSDDLHYAQIMADRLGIDLKVIEADSDFLSMLPDLAGFMEDGFSDPAAINTWLICAGAR
KQGIKVMLSGQGADEFLGGYRRYFAERALQRMPGFVRSALSATSGMLPANLPGKFNALNR
RFKRFANLAAQPPRDRILGMYSWATPATIGGLFAAPLASHPGEQFQALLDGLPPGDCVDT
LMAIDRQYDLLSLNLTYTDRMSMAVGVEARVPFLDFDLVRVMNAIPASLKVKGRQGKYIF
KKAMEPQLPHEIIYRSKAGFGLPIRAWMDKRNDLLNRYLNRARIEAQGIFNADAIERIRG
EQAAGAADHANTLLTLLTQQISLEKLGASAT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory