SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ga0059261_3276 Ga0059261_3276 ABC-type multidrug transport system, ATPase and permease components to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9JI39 ATP-binding cassette sub-family B member 10, mitochondrial; ABC-mitochondrial erythroid protein; ABC-me protein; ATP-binding cassette transporter 10; ABC transporter 10 protein from Mus musculus (Mouse) (see 2 papers)
39% identity, 86% coverage: 79:590/595 of query aligns to 183:698/715 of Q9JI39

query
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Q9JI39

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G497 (= G393) mutation to A: Decreases ATP binding about 50%.
K498 (= K394) mutation to R: Decreases ATP binding about 50%.
C547 (≠ A443) modified: S-glutathionyl cysteine; mutation to A: Does not affect ABCB10 glutathionylation.
G602 (= G495) mutation to D: Affects ATP hydrolysis but not binding.; mutation to V: Affects ATP hydrolysis but not binding.
E624 (= E517) mutation to Q: Affects ATP hydrolysis but not binding.
C675 (≠ V568) mutation to A: Prevents ABCB10 glutathionylation.

Sites not aligning to the query:

1:82 modified: transit peptide, Mitochondrion

Q9NRK6 ATP-binding cassette sub-family B member 10, mitochondrial; ABC-mitochondrial erythroid protein; ABC-me protein; ATP-binding cassette transporter 10; ABC transporter 10 protein; Mitochondrial ATP-binding cassette 2; M-ABC2 from Homo sapiens (Human) (see 5 papers)
39% identity, 86% coverage: 78:590/595 of query aligns to 217:733/738 of Q9NRK6

query
sites
Q9NRK6

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C224 (≠ V85) mutation to L: Does not affect ATPase activity; when associated withS-215 and G-582. Activated by Zn (II) mesoporphyrin; when associated with S-215 and G-582.
R471 (≠ Q332) to T: in a breast cancer sample; somatic mutation
K533 (= K394) mutation to E: Increases hemoglobin biosynthetic process.
D545 (= D406) to N: in dbSNP:rs35698797
C582 (≠ A443) mutation to G: Does not affect ATPase activity; when associated with S-215 and L-224. Activated by Zn (II) mesoporphyrin; when associated with S-215 and L-224.
S635 (= S493) mutation to R: Does not rescue hemoglobin and heme biosynthetic process.
Q638 (= Q496) mutation to H: Does not rescue hemoglobin and heme biosynthetic process.
D658 (= D516) mutation to A: Does not rescue hemoglobin and heme biosynthetic process.
E659 (= E517) mutation to A: Does not rescue hemoglobin and heme biosynthetic process.

Sites not aligning to the query:

150 A → S: in dbSNP:rs4148756
215 C→S: Does not affect ATPase activity; when associated with L-224 and G-582. Activated by Zn (II) mesoporphyrin; when associated with L-224 and G-582.

4ayxA Structure of the human mitochondrial abc transporter, abcb10 (rod form b) (see paper)
39% identity, 84% coverage: 78:579/595 of query aligns to 64:568/571 of 4ayxA

query
sites
4ayxA

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binding phosphomethylphosphonic acid adenylate ester: Y348 (= Y362), S376 (= S390), G377 (= G391), G379 (= G393), K380 (= K394), S381 (= S395), T382 (= T396)
binding glycine: Y81 (= Y95)
binding magnesium ion: S381 (= S395), Q422 (= Q436)

Sites not aligning to the query:

binding glycine: 17

Q9NUT2 Mitochondrial potassium channel ATP-binding subunit; ATP-binding cassette sub-family B member 8, mitochondrial; ABCB8; Mitochondrial ATP-binding cassette 1; M-ABC1; Mitochondrial sulfonylurea-receptor; MITOSUR from Homo sapiens (Human) (see 4 papers)
39% identity, 87% coverage: 79:595/595 of query aligns to 198:715/735 of Q9NUT2

query
sites
Q9NUT2

L

L

L

I

V

L

V

Y

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G

V

V

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x
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K

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G

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507:514 (vs. 388:395, 63% identical) binding
GK 512:513 (= GK 393:394) mutation to AR: Renders the protein unstable.
K513 (= K394) mutation to A: Abolish binding to ATP.
A690 (≠ T570) to G: in a breast cancer sample; somatic mutation

Sites not aligning to the query:

152 V → I: in dbSNP:rs4148844
165 I → T: in a breast cancer sample; somatic mutation

5ochE The crystal structure of human abcb8 in an outward-facing state
39% identity, 87% coverage: 79:594/595 of query aligns to 58:574/576 of 5ochE

query
sites
5ochE

L

L

L

I

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L

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Y

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|
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T

S

S

A

A

L

L

D

D

A

A

E

E

S

S

E

E

R

R

L

V

V

V

Q

Q

D

E

A

A

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D

H

R

L

A

M

S

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A

G

G

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R

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L

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V

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I

A

A

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H

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Y

A

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L

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D

A

A

binding adenosine-5'-diphosphate: Y341 (= Y362), C343 (≠ T364), G370 (= G391), G372 (= G393), K373 (= K394), T374 (≠ S395), T375 (= T396)
binding cholesterol hemisuccinate: P163 (= P184), F238 (≠ M259), S242 (≠ V263), N243 (≠ I264), F246 (≠ L267), M285 (≠ F306), L288 (= L309), V295 (≠ L316)

7y48B Cryo-em structure of biliverdin-bound mitochondrial abc transporter abcb10 from biortus
38% identity, 84% coverage: 78:579/595 of query aligns to 63:564/567 of 7y48B

query
sites
7y48B

G

G

L

L

L

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L

A

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G

A

A

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-

A

-

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x
T

A

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x
G

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x
I

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V

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I

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F

A

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C

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S

A

I

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A

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N

N

L

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Y

G

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D

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A

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A

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Q

E

I

I

W

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A

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A

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A

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N

A

A

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A

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K

binding Biliverdine IX Alpha: V171 (≠ I186), T248 (≠ R256), G252 (≠ T260), I255 (≠ V263)

Q9NP78 ABC-type oligopeptide transporter ABCB9; ATP-binding cassette sub-family B member 9; ATP-binding cassette transporter 9; ABC transporter 9 protein; hABCB9; TAP-like protein; TAPL; EC 7.4.2.6 from Homo sapiens (Human) (see 4 papers)
36% identity, 87% coverage: 76:590/595 of query aligns to 225:741/766 of Q9NP78

query
sites
Q9NP78

F

F

Q

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A

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N

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K

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A

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|
H

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H

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Q

K545 (= K394) mutation to A: Loss of peptide transport activity; whena ssociated with A-699.
H699 (= H548) mutation to A: Loss of peptide transport activity; whena ssociated with A-545.

Sites not aligning to the query:

17 Intramolecular salt bridge with Arg-57. Essential for the release from the ER; D→N: Loss of lysosomal localization. Does not affect interaction between coreABCB9 and TMD0 domains. Does not affect dimerization. Does not affect peptide transport activity. Decreases interaction with YIF1B.; D→R: Loss of lysosomal localization. Does not affect lysosomal localization; when associated with D-57. Does not affect interaction between coreABCB9 and TMD0 domains. Does not affect interaction between coreABCB9 and TMD0 domains; when associated with D-57. Does not affect interaction between coreABCB9 and TMD0 domains; when associated with D-100.
45 Important for the second trafficking step from the Golgi to the endosomal and lysosomal compartments; D→K: Loss of lysosomal localization; when assosiated with K-49. Loss of lysosomal localization; when assosiated with K-49 and D-100. Does not affect peptide transport activity; when assosiated with K-49 and D-100.; D→N: Decreases lysosomal localization; when associated with N-49.
49 Important for the second trafficking step from the Golgi to the endosomal and lysosomal compartments; D→K: Loss of lysosomal localization; when assosiated with K-45. Loss of lysosomal localization; when assosiated with K-45 and D-100. Does not affect peptide transport activity; when assosiated with K-45 and D-100.; D→N: Decreases lysosomal localization; when associated with N-45.
57 Intramolecular salt bridge with Asp-17. Essential for the release from the ER; R→A: Decreases lysosomal localization. Loss of lysosomal localization; when associated with A-100.; R→D: Loss of lysosomal localization. Does not affect lysosomal localization; when associated with R-17. Does not affect interaction between coreABCB9 and TMD0 domains. Does not affect interaction between coreABCB9 and TMD0 domains; when associated with R-17.
100 K→A: Decreases lysosomal localization. Loss of lysosomal localization; when associated with A-57.; K→D: Decreases lysosomal localization. Loss of lysosomal localization; when assosiated with R-17. Loss of lysosomal localization; when assosiated with K-45 and K-49. Does not affect peptide transport activity; when assosiated with K-45 and K-49. Does not affect interaction between coreABCB9 and TMD0 domains. Does not affect interaction between coreABCB9 and TMD0 domains; when associated with R-17.
121 V → M: in dbSNP:rs3803002
136:137 LL→AA: No effect on lysosomal localization.

7vfiA Cryo-em structure of the mouse tapl (9mer-peptide bound) (see paper)
37% identity, 81% coverage: 108:590/595 of query aligns to 89:569/570 of 7vfiA

query
sites
7vfiA

L

L

R

R

A

N

A

C

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x
Q

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|
R

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K

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W

I

L

Y

G

G

G

K

R

Y

V

Y

R

K

R

R

F

L

S

S

R

K

T

E

S

V

Q

Q

D

S

R

A

V

L

A

A

D

R

I

A

G

S

A

T

I

T

A

A

S

E

E

E

T

T

L

I

G

S

A

A

M

M

R

K

I

T

V

V

Q

R

A

S

F

F

G

A

Q

N

E

E

R

E

E

E

G

A

E

E

R

V

F

F

Q

L

T

R

A

K

V

L

D

Q

A

Q

G

V

F

Y

A

K

T

L

A

N

R

R

K

K

R

E

F

A

A

A

T

A

R
x
Y

A

M

L

S

M

Y

T

V

A

W

L

G

V

S

I

G

F

L

A

T

L

L

F

L

T

V

A

V

I

Q

T

V

L

S

I

I

M

L

W

Y

D

Y

A

G

V

G

Y

H

G

L

V

V

A

I

E

S

G

G

R

Q

I

M

S

S

G

S

G

G

S

N

I

L

T
x
I

A

A

F

F

V

I

I

I

T

Y

A

E

G
x
F

L

V

V

L

T

G

G

D

A

C

F

M

G

E

A

S

L

V

T

G

E

S

V

V

Y

Y

G

S

D

G

L

L

L

M

R

Q

A

G

S

V

G

G

A

A

S

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R

K

L

V

A

F

E

E

L

F

L

I

A

D

Q

R

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Q

P

P

D

T

I

M

A

V

A

H

P

D

A

G

N

S

P

L

V

A

P

P

L

D

P

H

V

L

P

E

P

-

L

-

G

G

A

R

I

V

D

D

F

F

K

E

N

N

V

V

T

T

F

F

R

T

Y

Y

P

R

T

T

R

R

P

P

E

H

V

T

S

Q

A

V

L

L

H

Q

Q

N

F

V

T

S

L

F

S

S

V

L

A

S

P

P

G

G

E

K

T

V

V

T

A

A

V

L

V

V

G

G

P

P

S

S

G

G

A

S

G

G

K

K

S

S

T

S

L

C

F

V

Q

N

L

I

V

L

Q

E

R

N

F

F

Y

Y

D

P

P

L

E

Q

G

G

E

R

I

V

R

L

V

L

D

D

G

G

I

K

P

P

V

I

R

G

S

A

A

Y

D

D

P

H

G

K

E

Y

V

L

R

H

A

R

R

V

M

I

A

S

M

L

V

V

P

S

Q

Q

E

E

T

P

V

V

I

L

F

F

A

A

A

R

T

S

A

I

R

T

D

D

N

N

L

I

R

S

Y

Y

G

G

R

L

W

P

D

T

A

V

T

P

E

F

E

E

Q

M

I

V

W

V

Q

E

A

A

E

Q

A

K

A

A

N

N

A

A

A

H

E

G

F

F

L

I

R

M

E

E

L

L

P

Q

Q

D

G

G

L

Y

E

S

T

T

F

E

L

T

G

G

E

E

D

K

G

G

A

A

R

Q

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

L

V

A

A

I

M

A

A

R

R

A

A

L

L

L

V

R

R

D

N

A

P

P

P

I

V

L

L

L

I

L

L

D

D

E

E

A

A

T

T

S

S

A

A

L

L

D

D

A

A

E

E

S

S

E

E

R

Y

L

L

V

I

Q

Q

D

Q

A

A

L

I

E

H

H

G

L

N

M

L

Q

Q

G

R

R

H

T

T

T

V

L

L

V

I

I

I

A

A

H

H

R

R

L

L

A

S

T

T

V

V

R

E

A

R

A

A

K

H

R

L

I

I

I

V

V

V

M

L

D

D

E

K

G

G

R

R

I

V

V

V

E

Q

T

Q

G

G

T

T

H

H

A

Q

E

Q

L

L

V

L

A

A

K

Q

G

G

L

L

Y

Y

A

A

K

K

L

L

A

V

S

Q

L

R

Q

Q

binding cholesterol hemisuccinate: R137 (= R156), I274 (≠ T293), F281 (≠ G300)
binding : Q130 (≠ T149), Y237 (≠ R256)

Sites not aligning to the query:

binding cholesterol hemisuccinate: 30, 41

7v5cA Cryo-em structure of the mouse abcb9 (adp.Bef3-bound) (see paper)
37% identity, 81% coverage: 108:590/595 of query aligns to 90:570/572 of 7v5cA

query
sites
7v5cA

L

L

R

R

A

N

A

C

V

L

H

F

R

R

N

S

L

L

L

V

R

S

L

Q

P

E

P

T

K

S

W

F

F

F

E

D

E

E

N

N

R

R

P

T

S

G

E

D

I

L

A

I

S

S

R

R

L

L

T

T

A

S

D

D

T

T

A

T

V

M

V

V

E

S

Q

D

I

L

V

V

G

S

T

Q

T

N

V

I

S

N

V

I

A

F

L

L

R

R

N

N

L

T

L

V

I

K

A

V

I

T

G

G

G

V

T

V

V

V

Y

F

L

M

F

F

V

S

L

L

A

S

P

W

K

Q

I

L

A

S

A

L

Y

V

L

T

I

F

I

M

G

G

I

F

P

P

V

I

I

I

V

M

L

M

P

V

I

S

M

N

W

I

L

Y

G

G

G

K

R

Y

V

Y

R

K

R

R

F

L

S

S

R

K

T

E

S

V

Q

Q

D

S

R

A

V

L

A

A

D

R

I

A

G

S

A

T

I

T

A

A

S

E

E

E

T

T

L

I

G

S

A

A

M

M

R

K

I

T

V

V

Q

R

A

S

F

F

G

A

Q

N

E

E

R

E

E

E

G

A

E

E

R

V

F

F

Q

L

T

R

A

K

V

L

D

Q

A

Q

G

V

F

Y

A

K

T

L

A

N

R

R

K

K

R

E

F

A

A

A

T

A

R

Y

A

M

L

S

M

Y

T

V

A

W

L

G

V

S

I

G

F

L

A

T

L

L

F

L

T

V

A

V

I

Q

T

V

L

S

I

I

M

L

W

Y

D

Y

A

G

V

G

Y

H

G

L

V

V

A

I

E

S

G

G

R

Q

I

M

S

S

G

S

G

G

S

N

I

L

T

I

A

A

F

F

V

I

I

I

T

Y

A

E

G

F

L

V

V

L

T

G

G

D

A

C

F

M

G

E

A

S

L

V

T

G

E

S

V

V

Y

Y

G

S

D

G

L

L

L

M

R

Q

A

G

S

V

G

G

A

A

S

E

R

K

L

V

A

F

E

E

L

F

L

I

A

D

Q

R

E

Q

P

P

D

T

I

M

A

V

A

H

P

D

A

G

N

S

P

L

V

A

P

P

L

D

P

H

V

L

P

E

P

-

L

-

G

G

A

R

I

V

D

D

F

F

K

E

N

N

V

V

T

T

F

F

R

T

Y
|
Y

P

R

T
|
T

R

R

P

P

E

H

V

T

S

Q

A

V

L

L

H

Q

Q

N

F

V

T

S

L

F

S

S

V

L

A

S

P

P

G

G

E

K

T

V

V

T

A

A

V

L

V

V

G

G

P

P

S
|
S

G

G

A
x
S

G

G

K
|
K

S
|
S

T
x
S

L

C

F

V

Q

N

L

I

V

L

Q

E

R

N

F

F

Y

Y

D

P

P

L

E

Q

G

G

E

R

I

V

R

L

V

L

D

D

G

G

I

K

P

P

V

I

R

G

S

A

A

Y

D

D

P

H

G

K

E

Y

V

L

R

H

A

R

R

V

M

I

A

S

M

L

V

V

P

S

Q
|
Q

E

E

T

P

V

V

I

L

F

F

A

A

A

R

T

S

A

I

R

T

D

D

N

N

L

I

R

S

Y

Y

G

G

R

L

W

P

D

T

A

V

T

P

E

F

E

E

Q

M

I

V

W

V

Q

E

A

A

E

Q

A

K

A

A

N

N

A

A

A

H

E

G

F

F

L

I

R

M

E

E

L

L

P

Q

Q

D

G

G

L

Y

E

S

T

T

F

E

L

T

G

G

E

E

D

K

G

G

A

A

R

Q

L

L

S
|
S

G

G

Q
|
Q

R

K

Q

Q

R

R

L

V

A

A

I

M

A

A

R

R

A

A

L

L

L

V

R

R

D

N

A

P

P

P

I

V

L

L

L

I

L

L

D

D

E

E

A

A

T

T

S

S

A

A

L

L

D

D

A

A

E

E

S

S

E

E

R

Y

L

L

V

I

Q

Q

D

Q

A

A

L

I

E

H

H

G

L

N

M

L

Q

Q

G

R

R

H

T

T

T

V

L

L

V

I

I

I

A

A

H
|
H

R

R

L

L

A

S

T

T

V

V

R

E

A

R

A

A

K

H

R

L

I

I

I

V

V

V

M

L

D

D

E

K

G

G

R

R

I

V

V

V

E

Q

T

Q

G

G

T

T

H

H

A

Q

E

Q

L

L

V

L

A

A

K

Q

G

G

L

L

Y

Y

A

A

K

K

L

L

A

V

S

Q

L

R

Q

Q

binding adenosine-5'-diphosphate: Y342 (= Y362), T344 (= T364), S372 (≠ A392), K374 (= K394), S375 (= S395), S376 (≠ T396), S473 (= S493), Q476 (= Q496)
binding beryllium trifluoride ion: S370 (= S390), K374 (= K394), Q416 (= Q436), H528 (= H548)
binding magnesium ion: S375 (= S395), Q416 (= Q436)

4aywA Structure of the human mitochondrial abc transporter, abcb10 (plate form) (see paper)
38% identity, 84% coverage: 78:575/595 of query aligns to 64:560/560 of 4aywA

query
sites
4aywA

G

G

L

L

L

S

L

A

V

V

V

F

V

L

V

C

L

G

A

A

F

A

A

A

T

N

A
|
A

M

I

R

R

F

V

Y
|
Y

F

L

V

M

S

Q

W

T

L

S

G

G

E

Q

R

R

T

I

V

V

A

N

D

R

L

L

R

R

A

T

A

S

V

L

H

F

R

S

N

S

L

I

L

L

R

R

L

Q

P

E

P

V

K

A

W

F

F

F

E

D

E

K

N

T

R

R

P

T

S

G

E

E

I

L

A

I

S

N

R

R

L

L

T

S

A

S

D

D

T

T

A

A

V

L

E

G

Q

R

I

S

V

V

G

T

T

E

T

N

V

L

S

S

V

D

A

G

L

L

R

R

N

A

L

G

L

A

I

Q

A

A

I

S

G

V

G

G

T

I

V

S

Y

M

L

M

F

F

V

F

L

V

A

S

P

P

K

N

I

L

A

A

A

T

Y

F

L

V

I

L

I

S

G

V

I

V

P

P

V

P

I

V

V

S

L

I

P

I

I

A

M

V

W

I

L

Y

G

G

G

R

R

Y

V

L

R

R

R

K

F

L

S

T

R

K

T

V

S

T

Q

Q

D

D

R

S

V

L

A

A

D

Q

I

A

G

T

A

Q

I

L

A

A

S

E

E

E

T

R

L

I

G

G

A

N

M

V

R

R

I

T

V

V

Q

R

A

A

F

F

G

G

Q

K

E

E

R

M

R

T

E

E

G

I

E

E

R

K

F

Y

Q

A

T

S

A

K

V

V

D

D

A

H

G

V

F

M

A

Q

T

L

A

A

R

R

K

K

R

E

-

A

-

F

-

A

-

R

-

A

-

G

-

F

F

F

A

G

T

A

R

T

A

G

L

L

M

S

T

G

A

N

L

L

V

I

I

V

F

L

A

S

L

V

F

L

T

Y

A

K

I

G

T

G

L

L

I

L

M

M

W

G

D

S

A

A

V

-

Y

-

G

-

V

-

A

-

E

-

G

-

R

H

I

M

S

T

G

V

G

G

S

E

I

L

T

S

A

S

F

F

V

L

I

M

T

Y

A

A

G

F

L

W

V

V

T

G

G

I

A

S

F

I

G

G

A

G

L

L

T

S

E

S

V

F

Y

Y

G

S

D

E

L

L

L

M

R

K

A

G

S

L

G

G

A

A

A

G

S

G

R

R

L

L

A

W

E

E

L

L

A

E

Q

R

E

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P

P

D

K

I

L

A

P

A

F

P

N

A

E

N

G

P

V

V

I

P

L

L

N

P

E

V

K

P

S

P

F

L

Q

G

G

A

A

I

L

D

E

F

F

K

K

N

N

V

V

T

H

F

F

R

A

Y
|
Y

P

P

T

A

R

R

P

P

E

E

V

V

S

P

A

I

L

F

H

Q

Q

D

F

F

T

S

L

L

S

S

V

I

A

P

P

S

G

G

E

S

T

V

V

T

A

A

V

L

V

V

G

G

P

P

S
|
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

V

F

L

Q

S

L

L

V

L

Q

L

R

R

F

L

Y

Y

D

D

P

P

E

A

G

S

G

G

E

T

I

I

R

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V

L

D

D

G

G

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R

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Q

A

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N

P

P

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W

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K

M

I

A

G

M

T

V

V

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Q

Q

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V

I

I

L

F

F

A

S

A

C

T

S

A

I

R

A

D

E

N

N

L

I

R

A

Y

Y

G

G

R

A

W

D

D

D

-

P

-

S

A

V

T

T

E

A

E

E

Q

E

I

I

W

Q

Q

R

A

V

A

A

E

E

A

V

A

A

N

N

A

A

A

V

E

A

F

F

L

I

R

R

E

N

L

F

P

P

Q

Q

G

G

L

F

E

N

T

T

F

V

L

V

G

G

E

E

D

K

G

G

A

V

R

L

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

L

I

A

A

I

I

A

A

R

R

A

A

L

L

R

K

D

N

A

P

P

K

I

I

L

L

D

D

E

E

A

-

T

-

S

-

A

-

L

L

D

D

A

A

E

E

S

N

E

E

R

Y

L

L

V

V

Q

Q

D

E

A

A

L

L

E

D

H

R

L

L

M

M

Q

D

G

G

R

R

T

T

T

V

L

L

V

V

I

I

A

A

H

H

R

H

L

L

A

S

T

T

V

I

R

K

A

N

A

A

K

N

R

M

I

V

I

A

V

V

M

L

D

D

E

Q

G

G

R

K

I

I

V

T

E

E

T

Y

G

G

T

K

H

H

A

E

E

E

binding phosphoaminophosphonic acid-adenylate ester: Y348 (= Y362), S376 (= S390), G377 (= G391), G379 (= G393), K380 (= K394), S381 (= S395), T382 (= T396)
binding cholesterol hemisuccinate: A77 (= A91), Y81 (= Y95)

Sites not aligning to the query:

binding cholesterol hemisuccinate: 17

7ehlA Cryo-em structure of human abcb8 transporter in nucleotide binding state (see paper)
38% identity, 85% coverage: 79:586/595 of query aligns to 62:558/563 of 7ehlA

query
sites
7ehlA

L

L

L

I

V

L

V

Y

V

G

V

V

L

Q

A

G

F

L

A

L

T

T

A

F

M

G

R

Y

F

L

Y

V

F

L

V

L

S

S

W

H

L

V

G

G

E

E

R

R

T

M

V

A

A

V

D

D

L

M

R

R

A

R

A

A

V

L

H

F

R

S

N

S

L

L

R

R

L

Q

P

D

P

I

K

T

W

F

F

F

E

D

E

A

N

N

R

K

P

T

S

G

E

Q

I

L

A

V

S

S

R

R

L

L

T

T

A

T

D

D

T

V

A

Q

V

E

V

F

E

K

Q

S

I

S

V

F

G

K

T

L

T

V

V

I

S

S

V

Q

A

G

L

L

R
|
R

N

S

L

C

L

-

I

T

A

Q

I

V

G

A

G

G

T

C

V

L

Y

V

L

S

F

L

V

S

L

M

A

L

P

T

K

R

I
x
L

A

T

A

L

Y

L

L

L

I

M

I

V

G

A

I

T

P

P

V

A

I
x
L

V

M

L

G

P

V

I
x
G

M

T

W

L

L

M

G

G

G

S

R

G

V

L

R

R

R

K

F

L

S

S

R

R

T

Q

S

C

Q

Q

D

E

R

Q

V

I

A

A

D

R

I

A

G

M

A

G

I

V

A

A

S

D

E

E

T

A

L

L

G

G

A

N

M

V

R

R

I

T

V

V

Q

R

A

A

F

F

G

A

Q

M

E

E

R

Q

R

R

E

E

G

E

E

E

R

R

F

Y

Q

G

T

A

A

E

V

L

D

E

A

A

G

C

F

R

A

C

T

R

A

A

R

E

K

E

R

L

F

G

A

R

T

G

R

I

A

A

L

L

M

F

T

Q

A

G

L

L

V

S

I

N

F

I

A

A

L

-

F

F

T

N

A

C

I

M

T

V

L

L

I

G

M

T

W

L

D

F

A

I

V
x
G

Y

G

G

S

V

L

A

V

E

A

G

G

R

Q

I

L

S

T

G

G

S

D

I
x
L

T

M

A

S

F
|
F

V

L

I

V

T

A

A

S

G

Q

L

T

V

V

T
x
Q

G
x
R

A

S

F

M

G

A

A

N

L

L

T

S

E

V

V

L

Y

F

G

G

D

Q

L

V

R

R

A

G

S

L

G

S

A

A

A

G

S

A

R

R

L

V

A

F

E

E

L

Y

L

M

A

A

Q

L

E

N

P

P

D

C

I

I

A

-

P

-

A

-

N

-

P

-

V

-

P

-

L

-

P

P

V

L

P

S

P

G

L

R

G

G

A

S

I

V

D

T

F

F

K

Q

N

N

V

V

T

C

F

F

R

S

Y
|
Y

P

P

T

C

R

R

P

P

E

G

V

F

S

E

A

V

L

L

H

K

Q

D

F

F

T

T

L

L

S

T

V

L

A

P

P

P

G

G

E

K

T

I

V

V

A

A

V

L

V

V

G

G

P

Q

S

S

G

G

A

G

G
|
G

K
|
K

S
x
T

T
|
T

L

V

F

A

Q

S

L

L

V

L

Q

E

R

R

F

F

Y

Y

D

D

P

P

E

T

G

A

G

G

E

V

I

V

R

M

V

L

D

D

G

G

I

R

P

D

V

L

R

R

S

T

A

L

D

D

P

P

G

S

E

W

V

L

R

R

A

G

R

Q

M

V

-

V

A

G

M

F

V

I

P

S

Q

Q

E

E

T

P

V

V

I

L

F

F

A

G

A

T

T

T

A

I

R

M

D

E

N

N

L

I

R

R

Y

F

G

G

R

K

W

L

D

E

A

A

T

S

E

D

E

E

Q

E

I

V

W

Y

Q

T

A

A

E

R

A

E

A

A

N

N

A

A

A

H

E

E

F

F

L

I

R

T

E

S

L

F

P

P

Q

E

G

G

L

Y

E

N

T

T

F

-

L

V

G

G

E

E

D

R

G

G

A

T

R

T

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

L

L

A

A

I

I

A

A

R

R

A

A

L

L

L

I

R

K

D

Q

A

P

P

T

I

V

L

L

L

I

L

L

D

D

E

E

A

A

T

T

S

S

A

A

L

L

D

D

A

A

E

E

S

S

E

E

R

R

L

V

V

V

Q

Q

D

E

A

A

L

L

E

D

H

R

L

A

M

S

Q

A

G

G

R

R

T

T

T

V

L

L

V

V

I

I

A

A

H

H

R

R

L

L

A

S

T

T

V

V

R

R

A

G

A

A

K

H

R

C

I

I

I

V

V

V

M

M

D

A

E

D

G

G

R

R

I

V

V

W

E

E

T

A

G

G

T

T

H

H

A

E

E

E

L

L

V

L

A

K

K

K

G

G

L

L

Y

Y

A

A

K

E

L

L

binding phosphoaminophosphonic acid-adenylate ester: Y334 (= Y362), G365 (= G393), K366 (= K394), T367 (≠ S395), T368 (= T396)
binding cholesterol: R139 (= R156), L157 (≠ I175), L168 (≠ I186), G172 (≠ I190), G260 (≠ V279), L273 (≠ I292), F276 (= F295), Q284 (≠ T303), R285 (≠ G304)

5ochC The crystal structure of human abcb8 in an outward-facing state
38% identity, 87% coverage: 79:594/595 of query aligns to 52:533/537 of 5ochC

query
sites
5ochC

L

L

L

I

V

L

V

Y

V

G

V

V

L

Q

A

G

F

L

A

L

T

T

A

F

M

G

R

Y

F

L

Y

V

F

L

V

L

S

S

W

H

L

V

G

G

E

E

R

R

T

M

V

A

A

V

D

D

L

M

R

R

A

R

A

A

V

L

H

F

R

S

N

S

L

L

R

R

L

Q

P

D

P

I

K

T

W

F

F

F

E

D

E

A

N

N

R

K

P

T

S

G

E

Q

I

L

A

V

S

S

R

R

L

L

T

T

A

T

D

D

T

V

A

Q

V

E

V

F

E

K

Q

S

I

S

V

F

G

K

T

L

T

V

V

I

S

S

V

Q

A

G

L

L

R

R

N

S

L

C

L

T

I

Q

A

V

I

T

G

R

G

L

T

T

V

L

Y

L

L

L

F

M

V

V

L

-

A

-

P

-

K

-

I

-

A

-

Y

-

L

-

I

-

G

-

I

-

P

-

V

-

I

-

V

-

L

-

P

-

I

-

M

-

W

-

L

-

G

G

G

S

R

G

V

L

R

R

R

K

F

L

S

S

R

R

T

Q

S

C

Q

Q

D

E

R

Q

V

I

A

A

D

R

I

A

G

M

A

G

I

V

A

A

S

D

E

E

T

A

L

L

G

G

A

N

M

V

R

R

I

T

V

V

Q

R

A

A

F

F

G

A

Q

M

E

E

R

Q

R

R

E

E

G

E

E

E

R

R

F

Y

Q

G

T

A

A

E

V

L

D

E

A

A

G

-

F

-

A

C

T

R

A

C

R

R

K

A

R

E

F

E

A

L

T

G

R

R

A

G

L

I

M

-

T

-

A

-

L

-

V

-

I

-

F

-

A

A

L

L

F

F

T

Q

A

G

I

L

T

S

L

N

I

I

M

A

W

F

D

N

A

C

V

M

Y

-

G

-

V

-

A

V

E

L

G

G

R

T

I

L

S

F

G

I

G

G

S

D

I

L

T

M

A

S

F

F

V

L

I

V

T

A

A

S

G

Q

L

T

V

V

T

Q

G

R

A

S

F

M

G

A

A

N

L

L

T

S

E

V

V

L

Y

F

G

G

D

Q

L

V

R

R

A

G

S

L

G

S

A

A

A

G

S

A

R

R

L

V

A

F

E

E

L

Y

L

M

A

A

Q

L

E

N

P

P

D

C

I

I

A

P

A

L

P

S

A

G

N

G

P

C

V

C

P

-

L

V

P

P

V

K

P

E

P

Q

L

L

-

R

G

G

A

S

I

V

D

T

F

F

K

Q

N

N

V

V

T

C

F

F

R

S

Y
|
Y

P

P

T
x
C

R

R

P

P

E

G

V

F

S

E

A

V

L

L

H

K

Q

D

F

F

T

T

L

L

S

T

V

L

A

P

P

P

G

G

E

K

T

I

V

V

A

A

V

L

V

V

G

G

P

Q

S

S

G
|
G

A

G

G
|
G

K
|
K

S
x
T

T
|
T

L

V

F

A

Q

S

L

L

V

L

Q

E

R

R

F

F

Y

Y

D

D

P

P

E

T

G

A

G

G

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V

I

V

R

M

V

L

D

D

G

G

I

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V

L

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T

A

L

D

D

P

P

G

S

E

W

V

L

R

R

A

G

R

Q

M

V

-

V

A

G

M

F

V

I

P

S

Q

Q

E

E

T

P

V

V

I

L

F

F

A

G

A

T

T

T

A

I

R

M

D

E

N

N

L

I

R

R

Y

F

G

G

R

K

W

L

D

E

A

A

T

S

E

D

E

E

Q

E

I

V

W

Y

Q

T

A

A

E

R

A

E

A

A

N

N

A

A

A

H

E

E

F

F

L

I

R

T

E

S

L

F

P

P

Q

E

G

G

L

Y

E

N

T

T

F

V

L

V

G

G

E

E

D

R

G

G

A

T

R

T

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

L

L

A

A

I

I

A

A

R

R

A

A

L

L

L

I

R

K

D

Q

A

P

P

T

I

V

L

L

L

I

L

L

D

D

E

E

A

A

T

T

S

S

A

A

L

L

D

D

A

A

E

E

S

S

E

E

R

R

L

V

V

V

Q

Q

D

E

A

A

L

L

E

D

H

R

L

A

M

S

Q

A

G

G

R

R

T

T

T

V

L

L

V

V

I

I

A

A

H

H

R

R

L

L

A

S

T

T

V

V

R

R

A

G

A

A

K

H

R

C

I

I

I

V

V

V

M

M

D

A

E

D

G

G

R

R

I

V

V

W

E

E

T

A

G

G

T

T

H

H

A

E

E

E

L

L

V

L

A

K

K

K

G

G

L

L

Y

Y

A

A

K

E

L

L

A

I

S

R

L

R

Q

Q

F

A

N

L

D

D

A

A

binding adenosine-5'-diphosphate: Y300 (= Y362), C302 (≠ T364), G329 (= G391), G331 (= G393), K332 (= K394), T333 (≠ S395), T334 (= T396)

7metA A. Baumannii msba in complex with tbt1 decoupler (see paper)
35% identity, 93% coverage: 35:586/595 of query aligns to 14:561/564 of 7metA

query
sites
7metA

G

G

L

V

A

A

I

L

V

L

A

V

L

L

I

I

-

G

-

F

-

G

L

M

A

N

A

S

G

A

A

T

T

E

L

V

W

S

I
x
V

P

A

R

K

T

L

F

I

K

K

E

F

V

I

D

D

K

-

G

-

F

-

G

-

A

A

D

I

A

Q

D

N

P

A

S

S

R

R

I

A

G

D

A

L

Y

D

F

W

Q

F

G

P

L

L

L

I

V

I

V

L

V

V

L

F

-

F

-

R

A

G

F

L

A

G

T

L

A

F

M

M

R

G

F

G

Y

Y

F

Y

V

T

S

A

W

V

L

I

G

S

E

R

R

S

T

L

V

V

A

F

D

S

L

I

R

R

A

Q

A

E

V

V

H

Y

R

A

N

K

L

L

R

R

L

L

P

P

P

A

K

Q

W

Y

F

Y

E

L

E

D

N

N

R

S

P

S

S

G

E

H

I

I

A

T

S

A

R

K

L

I

T

M

A

Y

D

N

T

V

A

E

V

Q

V

L

E

T

Q

A

I

A

V

S

G

S

T

E

T

S

V

L

S

K

V

T

A

I

L

V

R

R

N

D

L

G

L

M

I

I

A

T

I

L

G

G

G

L

T

L

V

G

Y

Y

L

L

F

F

V

Y

L

T

A

N

P

W

K

R

I

L

A

T

A

I

Y

C

L

I

I

M

I

V

G

F

I

L

P

P

V

I

I

I

V

G

L

I

P

L

I

V

M

R

W

K

L

A

G

S

G

K

R

R

V

M

R

R

R

K

F

L

S

S

R

M

T

Q

S

V

Q

Q

D

D

R

T

V

M

A

G

D

D

I

V

G

N

A

H

I

V

A

V

S

Q

E

E

T

S

L

I

G

N

A

G

M

N

R

A

I

V

V

V

Q

K

A

S

F

F

G

A

Q

G

E

E

R

E

R

S

E

E

G

Q

E

E

R

R

F

F

Q

Y

T

K

A

S

V

S

D

E

A

E

G

N

F

L

A

K

T

R

A

G

R

L

K

K

R

M

F

V

A

I

T

V

R

Q

A

N

L

L

M

N

T

S

A

P

L

V

V

V

I

Q

F

V

A

V

L
x
M

F

A

T

C

A

A

I

M

T

A

L

L

I

I

M

V

W

W

D

L

A

A

V

L

Y

R

G

P

V

Q

A

I

E

L

G

G

R

N

I

T

S

T

G

A

G

G

S

E

I

F

T

V

A

A

F

Y

V

I

I

T

T

A

A
|
A

G
|
G

L

L

V

L

T
x
S

G
x
K

A

P

F
x
V

G

K

A

N

L

L

T

T

E

D

V

V

Y

N

G

E

D

K

L

L

L

Q

R

R

A

G

S

L

G

A

A

A

S

H

R

S

L

V

A

F

E

E

L

L

A

-

Q

-

E

-

P

-

D

D

I

L

A

P

A

E

P

E

A

Q

N

N

P

S

V

G

P

E

L

L

P

K

V

P

P

Q

P

L

L

Q

G

G

A

A

I

I

D

R

F

F

K

D

N

H

V

V

T

V

F

L

R

N

Y

Y

P

A

T

D

R

G

P

T

E

Q

V

-

S

-

A

A

L

I

H

K

Q

D

F

F

T

S

L

L

S

D

V

I

A

R

P

P

G

G

E

E

T

T

V

V

A

A

V

L

V

V

G

G

P

R

S

S

G

G

A

A

G

G

K

K

S

T

T

S

L

L

F

V

Q

N

L

M

V

L

Q

V

R

R

F

F

Y

Q

D

E

P

V

E

S

G

S

G

G

E

Q

I

I

R

Y

V

L

D

D

G

D

I

L

P

P

V

I

R

R

S

D

A

I

D

E

P

L

G

S

E

S

V

L

R

R

A

T

R

Q

M

I

A

A

M

M

V

V

P

N

Q

Q

E

Q

T

V

V

V

I

L

F

F

A

N

A

R

T

T

A

V

R

R

D

E

N

N

L

I

R

A

Y

Y

G

G

R

Q

-

L

W

H

D

N

A

A

T

S

E

D

E

E

Q

D

I

V

W

I

Q

A

A

A

E

K

A

A

N

Y

A

A

A

H

E

D

F

F

L

I

R

M

E

N

L

L

P

P

Q

N

G

G

L

Y

E

D

T

T

F

V

L

L

G

G

E

A

D

Q

G

G

A

L

R

N

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

L

I

A

A

I

I

A

A

R

R

A

A

L

I

L

L

R

K

D

N

A

A

P

P

I

I

L

L

L

I

L

L

D

D

E

E

A

A

T

T

S

S

A

A

L

L

D

D

A

N

E

E

S

S

E

E

R

H

L

F

V

I

Q

Q

D

Q

A

A

L

F

E

D

H

E

L

A

M

M

Q

Q

G

D

R

R

T

T

L

I

V

V

I

I

A

A

H

H

R

R

L

L

A

S

T

T

V

I

R

E

A

N

A

A

K

D

R

R

I

I

I

V

V

V

M

M

D

D

E

R

G

G

R

Q

I

I

V

V

E

E

T

Q

G

G

T

T

H

H

A

Q

E

E

L

L

V

L

A

A

K

K

G

H

G

G

L

A

Y

Y

A

Y

K

Q

L

L

binding 2-(4-chlorobenzamido)-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylic acid: V33 (≠ I51), M247 (≠ L267), A279 (= A299), G280 (= G300), S283 (≠ T303), K284 (≠ G304), V286 (≠ F306)

6a6mA Crystal structure of an outward-open nucleotide-bound state of the eukaryotic abc multidrug transporter cmabcb1 (see paper)
38% identity, 82% coverage: 102:591/595 of query aligns to 90:588/589 of 6a6mA

query
sites
6a6mA

E

E

R

R

T

L

V

A

A

R

D

D

L

L

R

R

A

V

A

A

V

A

H

F

R

S

N

N

L

L

L

V

R

E

L

Q

P

D

P

V

K

T

W

Y

F

F

E

D

E

R

N

R

R

K

P

A

S

G

E

E

I

L

A

G

S

G

R

K

L

L

T

N

A

N

D

D

T

V

A

Q

V

V

V

I

E

-

Q

Q

I

Y

V

S

G

F

T

S

T

K

V

L

S

G

V

A

A

V

L

L

R

F

N

N

L

L

L

A

I

Q

A

C

I

V

G

V

G

G

T

I

V

I

Y

V

L

A

F

F

V

I

L

F

A

A

P

P

K

A

I

L

A

T

A

G

Y

V

L

L

I

I

I

A

G

L

I

S

P

P

V

L

I

V

V

V

L

L

-

A

-

G

-

A

P

Q

I

M

M

I

W

E

L

M

G

S

G

G

R

N

V

T

R

K

R

R

F

-

S

-

R

-

T

-

S

S

Q

S

D

E

R

A

V

Y

A

A

D

S

I

A

G

G

A

S

I

V

A

A

S

A

E

E

T

V

L

F

G

S

A

N

M

I

R

R

I

T

V

T

Q

K

A

A

F

F

G

E

Q

A

E

E

R

R

R

Y

E

E

G

T

E

Q

R

R

F

Y

Q

G

T

S

A

K

V

L

D

D

A

P

G

L

F

Y

A

R

T

L

A

G

R

R

K

R

R

R

F

Y

A

I

T

S

R

D

A

G

L

L

M

F

T

F

A

G

L

L

V

S

I

M

F

L

A

V

L

I

F

F

T

C

A

V

I

Y

T

A

L

L

I

A

M

L

W

W

D

W

A

G

V

G

Y

Q

G

L

V

I

A

A

E

R

G

G

R

S

I

L

S

N

G

L

G

G

S

N

I

L

T

L

A

A

F

A

V

F

I

F

T

S

A

A

G

I

L

L

V

G

T

F

G

M

A

G

F

V

G

G

A

Q

L

A

T

A

E

Q

V

V

Y

W

G

P

D

D

L

V

L

T

R

R

A

G

S

L

G

G

A

A

A

G

S

G

R

E

L

L

A

F

E

A

L

M

L

I

A

D

Q

R

E

V

P

P

D

Q

I

Y

A

R

P

P

-

D

-

P

-

G

A

A

N

E

P

V

V

V

P

T

L

Q

P

P

V

L

P

V

P

L

L

K

G

Q

A

G

I

I

D

V

F

F

K

E

N

N

V

V

T

H

F

F

R

R

Y
|
Y

P

P

T

T

R
|
R

P

M

E

N

V

V

S

E

A

V

L

L

H

R

Q

G

F

I

T

S

L

L

S

T

V

I

A

P

P

N

G

G

E

K

T

T

V

V

A

A

V

I

V

V

G
|
G

P
x
G

S
|
S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

I

F

I

Q

Q

L

L

V

L

Q

M

R

R

F

F

Y

Y

D

D

P

I

E

E

-

P

-

Q

-

G

G

G

E

L

I

L

R

L

V

F

D

D

G

G

I

T

P

P

V

A

R

W

S

N

A

Y

D

D

P

F

G

H

E

A

V

L

R

R

A

S

R

Q

M

I

A

G

M

L

V

V

P

S

Q
|
Q

E

E

T

P

V

V

I

L

F

F

A

S

A

G

T

T

A

I

R

R

D

D

N

N

L

I

R

L

Y

Y

G

G

R

K

W

R

D

D

A

A

T

T

E

D

E

E

Q

E

I

V

W

I

Q

Q

A

A

A

L

E

R

A

E

A

A

N

N

A

A

A

Y

E

S

F

F

L

V

R

M

E

A

L

L

P

P

Q

D

G

G

L

L

E

D

T

T

F

E

L

V

G

G

E

E

D

R

G

G

A

L

R

A

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

L

I

A

A

I

I

A

A

R

R

A

A

L

I

L

L

R

K

D

H

A

P

P

T

I

L

L

L

L

C

L

L

D

D

E

E

A

S

T

T

S

S

A

A

L

L

D

D

A

A

E

E

S

S

E

E

R

A

L

L

V

V

Q

Q

D

E

A

A

L

L

E

D

H

R

L

M

M

M

-

A

-

S

Q

D

G

G

R

V

T

T

T

S

L

V

V

V

I

I

A

A

H

H

R

R

L
|
L

A

S

T

T

V

V

R

A

A

R

A

A

K

D

R

L

I

I

I

L

V

V

M

M

D
x
Q

E
x
D

G

G

R

V

I

V

V

V

E

E

T

Q

G

G

T

N

H

H

A

S

E

E

L

L

V

M

A

A

K

L

G

G

-

P

-

S

G

G

L
x
F

Y

Y

A

Y

K

Q

L
|
L

A

V

S

E

L

K

Q

Q

F

L

binding phosphoaminophosphonic acid-adenylate ester: Y352 (= Y362), R355 (= R365), S380 (= S390), G383 (= G393), K384 (= K394), S385 (= S395), T386 (= T396), Q429 (= Q436)
binding decyl-beta-d-maltopyranoside: G378 (= G388), G379 (≠ P389), G381 (= G391), L545 (= L550), Q558 (≠ D563), D559 (≠ E564), F579 (≠ L582), L583 (= L586)
binding magnesium ion: S385 (= S395), Q429 (= Q436)

3wmgA Crystal structure of an inward-facing eukaryotic abc multidrug transporter g277v/a278v/a279v mutant in complex with an cyclic peptide inhibitor, acap (see paper)
38% identity, 82% coverage: 102:591/595 of query aligns to 89:587/589 of 3wmgA

query
sites
3wmgA

E

E

R

R

T

L

V

A

A

R

D

D

L

L

R

R

A

V

A

A

V

A

H

F

R

S

N

N

L

L

L

V

R

E

L

Q

P

D

P

V

K

T

W

Y

F

F

E

D

E

R

N

R

R

K

P

A

S

G

E

E

I

L

A

G

S

G

R

K

L

L

T

N

A

N

D

D

T

V

A

Q

V

V

V

I

E

-

Q

Q

I

Y

V

S

G

F

T

S

T

K

V

L

S

G

V

A

A

V

L

L

R

F

N

N

L

L

L

A

I

Q

A

C

I

V

G

V

G

G

T

I

V

I

Y

V

L

A

F

F

V

I

L

F

A

A

P

P

K

A

I

L

A

T

A

G

Y

V

L

L

I

I

I

A

G

L

I

S

P

P

V

L

I

V

V

V

L

L

P

A

I

V

M

V

W

V

L

Q

G

M

G

I

R

E

V

M

R

S

R

G

F

N

S

T

R

K

T

R

S

S

Q

S

D

E

R

A

V

Y

A

A

D

S

I

A

G

G

A

S

I

V

A

A

S

A

E

E

T

V

L

F

G

S

A

N

M

I

R

R

I

T

V

T

Q

K

A

A

F

F

G

E

Q

A

E

E

R

R

R

Y

E

E

G

T

E

Q

R

R

F

Y

Q

G

T

S

A

K

V

L

D

D

A

P

G

L

F

Y

A

R

T

L

A

G

R

R

K

R

R

R

F

Y

A

I

T

S

R

D

A

G

L

L

M

F

T

F

A

G

L

L

V

S

I

M

F

L

A

V

L

I

F

F

T

C

A

V

I

Y

T

A

L

L

I

A

M

L

W

W

D

W

A

G

V

G

Y

Q

G

L

V

I

A

A

E

R

G

G

R

S

I

L

S

N

G

L

G

G

S
x
N

I

L

T

L

A

T

F

A

V

F

I

F

T

S

A

A

G

I

L

L

V

G

T

F

G

M

A

G

F

V

G

G

A

Q

L

A

T

A

E

Q

V

V

Y

W

G

P

D

D

L

V

L

T

R

R

A

G

S

L

G

G

A

A

A

G

S

G

R

E

L

L

A

F

E

A

L

M

L

I

A

D

Q

R

E

V

P

P

D

Q

I

Y

A

R

P

P

-

D

-

P

-

G

A

A

N

E

P

V

V

V

P

T

L

Q

P

P

V

L

P

V

P

L

L

K

G

Q

A

G

I

I

D

V

F

F

K

E

N

N

V

V

T

H

F

F

R

R

Y

Y

P

P

T

T

R

R

P

M

E

N

V

V

S

E

A

V

L

L

H

R

Q

G

F

I

T

S

L

L

S

T

V

I

A

P

P

N

G

G

E

K

T

T

V

V

A

A

V

I

V

V

G

G

P

G

S

S

G

G

A

A

G

G

K

K

S

S

T

T

L

I

F

I

Q

Q

L

L

V

L

Q

M

R

R

F

F

Y

Y

D

D

P

I

E

E

-

P

-

Q

-

G

G

G

E

L

I

L

R

L

V

F

D

D

G

G

I

T

P

P

V

A

R

W

S

N

A

Y

D

D

P

F

G

H

E

A

V

L

R

R

A

S

R

Q

M

I

A

G

M

L

V

V

P

S

Q

Q

E

E

T

P

V

V

I

L

F

F

A

S

A

G

T

T

A

I

R

R

D

D

N

N

L

I

R

L

Y

Y

G

G

R

K

W

R

D

D

A

A

T

T

E

D

E

E

Q

E

I

V

W

I

Q

Q

A

A

A

L

E

R

A

E

A

A

N

N

A

A

A

Y

E

S

F

F

L

V

R

M

E

A

L

L

P

P

Q

D

G

G

L

L

E

D

T

T

F

E

L

V

G

G

E

E

D

R

G

G

A

L

R

A

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

L

I

A

A

I

I

A

A

R

R

A

A

L

I

L

L

R

K

D

H

A

P

P

T

I

L

L

L

L

C

L

L

D

D

E

E

A

S

T

T

S

S

A

A

L

L

D

D

A

A

E

E

S

S

E

E

R

A

L

L

V

V

Q

Q

D

E

A

A

L

L

E

D

H

R

L

M

M

M

-

A

-

S

Q

D

G

G

R

V

T

T

T

S

L

V

V

V

I

I

A

A

H

H

R

R

L

L

A

S

T

T

V

V

R

A

A

R

A

A

K

D

R

L

I

I

I

L

V

V

M

M

D

Q

E

D

G

G

R

V

I

V

V

V

E

E

T

Q

G

G

T

N

H

H

A

S

E

E

L

L

V

M

A

A

K

L

G

G

-

P

-

S

G

G

L

F

Y

Y

A

Y

K

Q

L

L

A

V

S

E

L

K

Q

Q

F

L

binding : N277 (≠ S291)

Sites not aligning to the query:

binding decyl-beta-d-maltopyranoside: 6, 13, 13, 18
binding : 43, 61, 62

7fc9A Crystal structure of cmabcb1 in lipidic mesophase revealed by lcp-sfx (see paper)
39% identity, 82% coverage: 102:586/595 of query aligns to 90:583/584 of 7fc9A

query
sites
7fc9A

E

E

R

R

T

L

V

A

A

R

D

D

L

L

R

R

A

V

A

A

V

A

H

F

R

S

N

N

L

L

L

V

R

E

L

Q

P

D

P

V

K

T

W

Y

F

F

E

D

E

R

N

R

R

K

P

A

S

G

E
|
E

I

L

A

G

S

G

R

K

L

L

T

N

A

N

D
|
D

T

V

A

Q

V

V

V

I

E

-

Q

Q

I

Y

V

S

G

F

T

S

T

K

V

L

S

G

V

A

A

V

L

L

R

F

N

N

L

L

L

A

I

Q

A

C

I

V

G

V

G

G

T

I

V

I

Y

V

L

A

F

F

V

I

L

F

A

A

P

P

K

A

I

L

A

T

A

G

Y

V

L

L

I

I

I

A

G

L

I

S

P

P

V

L

I

V

V

V

L

L

-

A

-

G

-

A

P

Q

I

M

M

I

W

E

L

M

G

S

G

G

R

N

V
x
T

R

K

R

R

F

-

S

-

R

-

T

-

S

S

Q
x
S

D
x
E

R

A

V

Y

A

A

D

S

I

A

G

G

A

S

I

V

A

A

S

A

E

E

T

V

L

F

G

S

A

N

M

I

R

R

I

T

V

T

Q

K

A

A

F

F

G

E

Q

A

E

E

R

R

R

Y

E

E

G

T

E

Q

R

R

F

Y

Q

G

T

S

A

K

V

L

D

D

A

P

G

L

F

Y

A

R

T

L

A

G

R

R

K

R

R

R

F

Y

A

I

T

S

R

D

A

G

L

L

M

F

T

F

A

G

L

L

V

S

I

M

F

L

A

V

L

I

F

F

T

C

A

V

I

Y

T

A

L

L

I

A

M

L

W

W

D

W

A

G

V

G

Y

Q

G

L

V

I

A

A

E

R

G

G

R

S

I

L

S

N

G

L

G

G

S

N

I

L

T

L

A

A

F

A

V

F

I

F

T

S

A

A

G

I

L

L

V

G

T

F

G

M

A

G

F

V

G

G

A

Q

L

A

T

A

E

Q

V

V

Y

W

G

P

D

D

L

V

L

T

R

R

A

G

S

L

G

G

A

A

A

G

S

G

R
x
E

L

L

A

F

E

A

L

M

L

I

A

D

Q

R

E

V

P

P

D

Q

I

Y

A

R

P

P

-

D

-

P

-

G

A

A

N

E

P

V

V

V

P

T

L

Q

P

P

V

L

P

V

P

L

L

K

G

Q

A

G

I

I

D

V

F

F

K

E

N
|
N

V

V

T
x
H

F

F

R

R

Y
|
Y

P

P

T

T

R
|
R

P

M

E

N

V

V

S
x
E

A

V

L

L

H

R

Q

G

F

I

T

S

L

L

S

T

V

I

A

P

P

N

G

G

E

K

T

T

V

V

A

A

V

I

V

V

G

G

P

G

S
|
S

G

G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

I

F

I

Q

Q

L

L

V

L

Q

M

R

R

F

F

Y

Y

D

D

P

I

E

E

-

P

-

Q

-

G

G

G

E

L

I

L

R

L

V

F

D

D

G

G

I

T

P

P

V

A

R

W

S

N

A

Y

D

D

P

F

G

H

E

A

V

L

R

R

A

S

R

Q

M

I

A

G

M

L

V

V

P

S

Q
|
Q

E

E

T

P

V

V

I

L

F

F

A

S

A

G

T

T

A

I

R

R

D

D

N

N

L

I

R

L

Y

Y

G

G

R

K

W

R

D

D

A

A

T

T

E
x
D

E
|
E

Q

E

I

V

W

I

Q

Q

A

A

A

L

E

R

A

E

A

A

N

N

A

A

A

Y

E

S

F

F

L

V

R

M

E

A

L

L

P

P

Q

D

G

G

L

L

E

D

T

T

F

E

L

V

G

G

E

E

D

R

G

G

A

L

R

A

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

L

I

A

A

I

I

A

A

R

R

A

A

L

I

L

L

R

K

D

H

A

P

P

T

I

L

L

L

L

C

L

L

D

D

E
|
E

A

S

T

T

S

S

A

A

L

L

D

D

A

A

E

E

S

S

E

E

R

A

L

L

V

V

Q

Q

D

E

A

A

L

L

E

D

H

R

L

M

M

M

-

A

-

S

Q

D

G

G

R

V

T

T

T

S

L

V

V

V

I

I

A

A

H
|
H

R

R

L

L

A

S

T

T

V

V

R

A

A

R

A

A

K

D

R

L

I

I

I

L

V

V

M

M

D

Q

E

D

G

G

R

V

I

V

V

V

E

E

T

Q

G

G

T

N

H

H

A

S

E

E

L

L

V

M

A

A

K

L

G

G

-

P

-

S

G

G

L

F

Y

Y

A

Y

K

Q

L

L

binding phosphoaminophosphonic acid-adenylate ester: Y352 (= Y362), R355 (= R365), S380 (= S390), G383 (= G393), K384 (= K394), S385 (= S395), T386 (= T396), Q429 (= Q436), H543 (= H548)
binding magnesium ion: D127 (= D139), T188 (≠ V197), S192 (≠ Q205), E312 (≠ R325), S385 (= S395), Q429 (= Q436)
binding zinc ion: E119 (= E131), D127 (= D139), S192 (≠ Q205), E193 (≠ D206), N347 (= N357), H349 (≠ T359), H349 (≠ T359), E359 (≠ S369), D451 (≠ E458), E452 (= E459), E510 (= E517), H543 (= H548)

Sites not aligning to the query:

binding zinc ion: 31, 74

6qexA Nanodisc reconstituted human abcb1 in complex with uic2 fab and taxol (see paper)
35% identity, 90% coverage: 57:590/595 of query aligns to 66:598/1182 of 6qexA

query
sites
6qexA

E

D

V

I

V

N

D

D

K

T

G

G

F

F

-

F

-

M

G

N

A

L

D

E

A

E

D

D

P

M

S
x
T

R

R

I

Y

G

A

A

Y

Y
|
Y

F

Y

Q

S

G
|
G

L

I

L

G

L

A

V

G

V

V

V

L

V

V

L

A

A

A

F

Y

A

-

T

-

A

-

M

I

R

Q

F

V

Y

S

F

F

V

W

S

C

W

L

L

A

G

A

E

G

R

R

T

Q

V

I

A

H

D

K

L

I

R

R

A

K

A

Q

V

F

H

F

R

H

N

A

L

I

L

M

R

R

L

Q

P

E

P

I

K

G

W

W

F

F

E

D

E

V

N

H

R

D

P

V

S

G

E

E

I

L

A

N

S

T

R

R

L

L

T

T

A

D

D

D

T

V

A

S

V

K

V

I

E

N

Q

E

I

G

V

I

G

G

T

D

T

K

V

I

S

G

V

M

A

F

L

F

R

Q

N

S

L

M

L

A

I

T

A

F

I

F

G

T

G

G

T

F

V

I

Y

V

L

G

F

F

V

T

L

R

A

G

P

W

K

K

I

L

A

T

A

L

Y

V

L

I

I

L

I
x
A

G

I

I

S

P

P

V

V

I

L

V

G

L

L

P

S

I

A

M

A

W

V

L

W

G

A

G

K

R

I

V

L

R

S

F

F

S

T

R

D

T

K

S

E

Q

L

D

L

R

A

V

Y

A

A

D

K

I

A

G

G

A

A

I

V

A

A

S

E

E

E

T

V

L

L

G

A

A

A

M

I

R

R

I

T

V

V

Q

I

A

A

F

F

G

G

Q

G

E

Q

R

K

E

E

G

L

E

E

R

R

F

Y

Q

N

T

K

A

N

V

L

D

E

A

E

G

A

F

K

A

R

T

I

A

G

R

I

K

K

R

K

F

A

A

I

T

T

R

A

A

N

L

I

M

S

T

I

A

G

L

A

V

A

I

F

F

L

A

L

L

I

F
x
Y

T

A

A

S

I
x
Y

T

A

L
|
L

I

A

M

F

W

W

D

Y

A

G

V

T

Y

T

G

L

V

V

A

L

E

S

G

G

R

E

I

Y

S

S

G

I

G

G

S

Q

I

V

T

L

A

T

F

V

V

F

I
x
F

T

S

A

-

G

-

L

V

V

L

T

I

G

G

A

A

F
|
F

-
x
S

-

V

G

G

A
x
Q

L

A

T

S

E

P

V

S

Y

I

G

E

D

A

L

F

L

A

R

N

A

A

S

R

G

G

A

A

S

Y

R

E

L

I

A

F

E

K

L

I

A

D

Q

N

E

K

P

P

D

S

I

I

A

D

A

S

P

Y

A

S

N

K

P

S

V

G

P

H

L

K

P

P

V

D

P

N

P

I

L

K

G

G

A

N

I

L

D

E

F

F

K

R

N

N

V

V

T

H

F

F

R

S

Y

Y

P

P

T

S

R

R

P

K

E

E

V

V

S

K

A

I

L

L

H

K

Q

G

F

L

T

N

L

L

S

K

V

V

A

Q

P

S

G

G

E

Q

T

T

V

V

A

A

V

L

V

V

G

G

P

N

S

S

G

G

A

C

G

G

K

K

S

S

T

T

L

T

F

V

Q

Q

L

L

V

M

Q

Q

R

R

F

L

Y

Y

D

D

P

P

E

T

G

E

G

G

E

M

I

V

R

S

V

V

D

D

G

G

I

Q

P

D

V

I

R

R

S

T

A

I

D

N

P

V

G

R

E

F

V

L

R

R

A

E

R

I

M

I

A

G

M

V

V

V

P

S

Q

Q

E

E

T

P

V

V

I

L

F

F

A

A

A

T

T

T

A

I

R

A

D

E

N

N

L

I

R

R

Y

Y

G

G

R

R

W

E

D

N

A

V

T

T

E

M

E

D

Q

E

I

I

W

E

Q

K

A

A

A

V

E

K

A

E

A

A

N

N

A

A

A

Y

E

D

F

F

L

I

R

M

E

K

L

L

P

P

Q

H

G

K

L

F

E

D

T

T

F

L

L

V

G

G

E

E

D

R

G

G

A

A

R

Q

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

L

I

A

A

I

I

A

A

R

R

A

A

L

L

L

V

R

R

D

N

A

P

P

K

I

I

L

L

D

D

E

E

A

A

T

T

S

S

A

A

L

L

D

D

A

T

E

E

S

S

E

E

R

A

L

V

V

V

Q

Q

D

V

A

A

L

L

E

D

H

K

L

A

M

R

Q

K

G

G

R

R

T

T

L

I

V

V

I

I

A

A

H

H

R

R

L

L

A

S

T

T

V

V

R

R

A

N

A

A

K

D

R

V

I

I

I

A

V

G

M

F

D

D

E

D

G

G

R

V

I

I

V

V

E

E

T

K

G

G

T

N

H

H

A

D

E

E

L

L

V

M

A

K

K

E

G

K

G

G

L

I

Y

Y

A

F

K

K

L

L

A

V

S

T

L

M

Q

Q

binding cholesterol: T81 (≠ S70), Y86 (= Y75), G89 (= G78), A189 (≠ I181), L281 (= L273)
binding taxol: Y276 (≠ F268), Y279 (≠ I271), F305 (≠ I297), F312 (= F306), S313 (vs. gap), Q316 (≠ A308)

Sites not aligning to the query:

binding cholesterol: 15, 18, 36, 43, 745, 749, 751, 755, 757, 761, 762, 765, 769, 772, 775, 854, 857, 861, 864, 865
binding taxol: 38, 631, 852, 859, 889, 892, 896

7a6fA Nanodisc reconstituted human abcb1 in complex with mrk16 fab and zosuquidar (see paper)
35% identity, 88% coverage: 66:590/595 of query aligns to 59:580/1164 of 7a6fA

query
sites
7a6fA

D

E

A

E

D

D

P

M

S

T

R

R

I

Y

G

A

A

Y

Y

Y

F

Y

Q

S

G

G

L

I

L

G

L

A

V

G

V

V

V

L

V

V

L

A

A

A

F

Y

A

-

T

-

A

-

M

I

R

Q

F

V

Y

S

F

F

V

W

S

C

W

L

L

A

G

A

E

G

R

R

T

Q

V

I

A

H

D

K

L

I

R

R

A

K

A

Q

V

F

H

F

R

H

N

A

L

I

L

M

R

R

L

Q

P

E

P

I

K

G

W

W

F

F

E

D

E

V

N

H

R

D

P

V

S

G

E

E

I

L

A

N

S

T

R

R

L

L

T

T

A

D

D

D

T

V

A

S

V

K

V

I

E

N

Q

E

I

G

V

I

G

G

T

D

T

K

V

I

S

G

V

M

A

F

L

F

R

Q

N

S

L

M

L

A

I

T

A

F

I

F

G

T

G

G

T

F

V

I

Y

V

L

G

F

F

V

T

L

R

A

G

P

W

K

K

I

L

A

T

A

L

Y

V

L

I

I

L

I

A

G

I

I

S

P

P

V

V

I

L

V

G

L

L

P

S

I

A

M

A

W

V

L

W

G

A

G

K

R

I

V

L

R

S

F

F

S

T

R

D

T

K

S

E

Q

L

D

L

R

A

V

Y

A

A

D

K

I

A

G

G

A

A

I

V

A

A

S

E

E

E

T

V

L

L

G

A

A

A

M

I

R

R

I

T

V

V

Q

I

A

A

F

F

G

G

Q

G

E

Q

R

K

E

E

G

L

E

E

R

R

F

Y

Q

N

T

K

A

N

V

L

D

E

A

E

G

A

F

K

A

R

T

I

A

G

R

I

K

K

R

K

F

A

A

I

T

T

R

A

A

N

L

I

M

S

T

I

A

G

L

A

V

A

I
x
F

F

L

A

L

L
x
I

F

Y

T
x
A

A

S

I

Y

T

A

L

L

I

A

M

F

W
|
W

D

Y

A

G

V

T

Y

T

G

L

V

V

A

L

E

S

G

G

R

E

I

Y

S

S

G

I

G

G

S

Q

I

V

T

L

A

T

F

V

V

F

I
x
F

T

S

A

-

G

-

L

V

V
x
L

T

I

G

G

A

A

F
|
F

-

S

-

V

G

G

A

Q

L

A

T

S

E

P

V

S

Y

I

G

E

D

A

L

F

L

A

R

N

A

A

S

R

G

G

A

A

S

Y

R

E

L

I

A

F

E

K

L

I

A

D

Q

N

E

K

P

P

D

S

I

I

A

D

A

S

P

Y

A

S

N

K

P

S

V

G

P

H

L

K

P

P

V

D

P

N

P

I

L

K

G

G

A

N

I

L

D

E

F

F

K

R

N

N

V

V

T

H

F

F

R

S

Y

Y

P

P

T

S

R

R

P

K

E

E

V

V

S

K

A

I

L

L

H

K

Q

G

F

L

T

N

L

L

S

K

V

V

A

Q

P

S

G

G

E

Q

T

T

V

V

A

A

V

L

V

V

G

G

P

N

S

S

G

G

A

C

G

G

K

K

S

S

T

T

L

T

F

V

Q

Q

L

L

V

M

Q

Q

R

R

F

L

Y

Y

D

D

P

P

E

T

G

E

G

G

E

M

I

V

R

S

V

V

D

D

G

G

I

Q

P

D

V

I

R

R

S

T

A

I

D

N

P

V

G

R

E

F

V

L

R

R

A

E

R

I

M

I

A

G

M

V

V

V

P

S

Q

Q

E

E

T

P

V

V

I

L

F

F

A

A

A

T

T

T

A

I

R

A

D

E

N

N

L

I

R

R

Y

Y

G

G

R

R

W

E

D

N

A

V

T

T

E

M

E

D

Q

E

I

I

W

E

Q

K

A

A

A

V

E

K

A

E

A

A

N

N

A

A

A

Y

E

D

F

F

L

I

R

M

E

K

L

L

P

P

Q

H

G

K

L

F

E

D

T

T

F

L

L

V

G

G

E

E

D

R

G

G

A

A

R

Q

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

L

I

A

A

I

I

A

A

R

R

A

A

L

L

L

V

R

R

D

N

A

P

P

K

I

I

L

L

D

D

E

E

A

A

T

T

S

S

A

A

L

L

D

D

A

T

E

E

S

S

E

E

R

A

L

V

V

V

Q

Q

D

V

A

A

L

L

E

D

H

K

L

A

M

R

Q

K

G

G

R

R

T

T

L

I

V

V

I

I

A

A

H

H

R

R

L

L

A

S

T

T

V

V

R

R

A

N

A

A

K

D

R

V

I

I

I

A

V

G

M

F

D

D

E

D

G

G

R

V

I

I

V

V

E

E

T

K

G

G

T

N

H

H

A

D

E

E

L

L

V

M

A

K

K

E

G

K

G

G

L

I

Y

Y

A

F

K

K

L

L

A

V

S

T

L

M

Q

Q

binding cholesterol: A259 (≠ T269), W266 (= W276)
binding Zosuquidar: F254 (≠ I264), I257 (≠ L267), F287 (≠ I297), L290 (≠ V302), F294 (= F306)

Sites not aligning to the query:

binding cholesterol: 607, 615, 618, 646, 727, 731, 744, 747, 748
binding Zosuquidar: 34, 34, 613, 764, 767, 838, 841, 874, 878, 879

7a6eA Nanodisc reconstituted human abcb1 in complex with mrk16 fab and tariquidar (see paper)
35% identity, 88% coverage: 66:590/595 of query aligns to 59:580/1164 of 7a6eA

query
sites
7a6eA

D

E

A

E

D

D

P

M

S

T

R

R

I

Y

G

A

A

Y

Y
|
Y

F

Y

Q

S

G

G

L
x
I

L

G

L

A

V

G

V

V

V

L

V

V

L

A

A

A

F

Y

A

-

T

-

A

-

M

I

R

Q

F

V

Y

S

F

F

V

W

S

C

W

L

L

A

G

A

E

G

R

R

T

Q

V

I

A

H

D

K

L

I

R

R

A

K

A

Q

V

F

H

F

R

H

N

A

L

I

L

M

R

R

L

Q

P

E

P

I

K

G

W

W

F

F

E

D

E

V

N

H

R

D

P

V

S

G

E

E

I

L

A

N

S

T

R

R

L

L

T

T

A

D

D

D

T

V

A

S

V

K

V

I

E

N

Q

E

I

G

V

I

G

G

T

D

T

K

V

I

S

G

V

M

A

F

L

F

R

Q

N

S

L

M

L

A

I

T

A

F

I

F

G
x
T

G

G

T

F

V

I

Y

V

L

G

F

F

V
x
T

L

R

A

G

P

W

K

K

I

L

A

T

A
x
L

Y
x
V

L

I

I

L

I
x
A

G
x
I

I

S

P

P

V

V

I

L

V

G

L

L

P

S

I

A

M

A

W

V

L
x
W

G

A

G

K

R

I

V

L

R

S

F

F

S

T

R

D

T

K

S

E

Q

L

D

L

R

A

V

Y

A

A

D

K

I

A

G

G

A

A

I

V

A

A

S

E

E

E

T

V

L

L

G

A

A

A

M

I

R

R

I

T

V

V

Q

I

A

A

F

F

G

G

Q

G

E

Q

R

K

E

E

G

L

E

E

R

R

F

Y

Q

N

T

K

A

N

V

L

D

E

A

E

G

A

F

K

A

R

T

I

A

G

R

I

K

K

R

K

F

A

A

I

T

T

R

A

A

N

L

I

M

S

T

I

A

G

L

A

V

A

I
x
F

F

L

A

L

L
x
I

F
x
Y

T
x
A

A

S

I
x
Y

T

A

L

L

I

A

M

F

W
|
W

D
x
Y

A

G

V

T

Y

T

G

L

V

V

A

L

E

S

G

G

R

E

I

Y

S

S

G

I

G

G

S

Q

I

V

T

L

A

T

F

V

V

F

I
x
F

T

S

A

-

G

-

L

V

V

L

T

I

G

G

A

A

F

F

-

S

-

V

G

G

A
x
Q

L

A

T

S

E

P

V

S

Y

I

G

E

D

A

L

F

L

A

R

N

A

A

S

R

G

G

A

A

S

Y

R

E

L

I

A

F

E

K

L

I

A

D

Q

N

E

K

P

P

D

S

I

I

A

D

A

S

P

Y

A

S

N

K

P

S

V

G

P

H

L

K

P

P

V

D

P

N

P

I

L

K

G

G

A

N

I

L

D

E

F

F

K

R

N

N

V

V

T

H

F

F

R

S

Y

Y

P

P

T

S

R

R

P

K

E

E

V

V

S

K

A

I

L

L

H

K

Q

G

F

L

T

N

L

L

S

K

V

V

A

Q

P

S

G

G

E

Q

T

T

V

V

A

A

V

L

V

V

G

G

P

N

S

S

G

G

A

C

G

G

K

K

S

S

T

T

L

T

F

V

Q

Q

L

L

V

M

Q

Q

R

R

F

L

Y

Y

D

D

P

P

E

T

G

E

G

G

E

M

I

V

R

S

V

V

D

D

G

G

I

Q

P

D

V

I

R

R

S

T

A

I

D

N

P

V

G

R

E

F

V

L

R

R

A

E

R

I

M

I

A

G

M

V

V

V

P

S

Q

Q

E

E

T

P

V

V

I

L

F

F

A

A

A

T

T

T

A

I

R

A

D

E

N

N

L

I

R

R

Y

Y

G

G

R

R

W

E

D

N

A

V

T

T

E

M

E

D

Q

E

I

I

W

E

Q

K

A

A

A

V

E

K

A

E

A

A

N

N

A

A

A

Y

E

D

F

F

L

I

R

M

E

K

L

L

P

P

Q

H

G

K

L

F

E

D

T

T

F

L

L

V

G

G

E

E

D

R

G

G

A

A

R

Q

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

L

I

A

A

I

I

A

A

R

R

A

A

L

L

L

V

R

R

D

N

A

P

P

K

I

I

L

L

D

D

E

E

A

A

T

T

S

S

A

A

L

L

D

D

A

T

E

E

S

S

E

E

R

A

L

V

V

V

Q

Q

D

V

A

A

L

L

E

D

H

K

L

A

M

R

Q

K

G

G

R

R

T

T

L

I

V

V

I

I

A

A

H

H

R

R

L

L

A

S

T

T

V

V

R

R

A

N

A

A

K

D

R

V

I

I

I

A

V

G

M

F

D

D

E

D

G

G

R

V

I

I

V

V

E

E

T

K

G

G

T

N

H

H

A

D

E

E

L

L

V

M

A

K

K

E

G

K

G

G

L

I

Y

Y

A

F

K

K

L

L

A

V

S

T

L

M

Q

Q

binding cholesterol: Y68 (= Y75), I72 (≠ L79), T153 (≠ G163), T160 (≠ V170), L167 (≠ A177), V168 (≠ Y178), A171 (≠ I181), I172 (≠ G182), A259 (≠ T269), W266 (= W276), Y267 (≠ D277)
binding tariquidar: W183 (≠ L193), F254 (≠ I264), I257 (≠ L267), Y258 (≠ F268), Y261 (≠ I271), F287 (≠ I297), Q298 (≠ A308)

Sites not aligning to the query:

binding cholesterol: 43, 645, 727, 731, 732, 736, 843, 846
binding tariquidar: 34, 41, 613, 616, 763, 837, 841, 866, 867, 871, 874, 878

7a6cA Nanodisc reconstituted human abcb1 in complex with mrk16 fab and elacridar (see paper)
35% identity, 88% coverage: 66:590/595 of query aligns to 59:580/1164 of 7a6cA

query
sites
7a6cA

D

E

A

E

D

D

P

M

S

T

R

R

I

Y

G

A

A

Y

Y

Y

F

Y

Q

S

G

G

L

I

L

G

L

A

V

G

V

V

V

L

V

V

L

A

A

A

F

Y

A

-

T

-

A

-

M

I

R

Q

F

V

Y

S

F

F

V

W

S

C

W

L

L

A

G

A

E

G

R

R

T

Q

V

I

A

H

D

K

L

I

R

R

A

K

A

Q

V

F

H

F

R

H

N

A

L

I

L

M

R

R

L

Q

P

E

P

I

K

G

W

W

F

F

E

D

E

V

N

H

R

D

P

V

S

G

E

E

I

L

A

N

S

T

R

R

L

L

T

T

A

D

D

D

T

V

A

S

V

K

V

I

E

N

Q

E

I

G

V

I

G

G

T

D

T

K

V

I

S

G

V

M

A

F

L

F

R

Q

N

S

L

M

L

A

I

T

A

F

I

F

G

T

G

G

T

F

V

I

Y

V

L

G

F

F

V

T

L

R

A

G

P

W

K

K

I

L

A

T

A

L

Y

V

L

I

I

L

I

A

G

I

I

S

P

P

V

V

I

L

V

G

L

L

P

S

I

A

M

A

W

V

L
x
W

G

A

G

K

R

I

V
x
L

R

S

F
|
F

S

T

R

D

T

K

S

E

Q

L

D

L

R

A

V

Y

A

A

D

K

I

A

G

G

A

A

I

V

A

A

S

E

E

E

T

V

L

L

G

A

A

A

M

I

R

R

I

T

V

V

Q

I

A

A

F

F

G

G

Q

G

E

Q

R

K

E

E

G

L

E

E

R

R

F

Y

Q

N

T

K

A

N

V

L

D

E

A

E

G

A

F

K

A

R

T

I

A

G

R

I

K

K

R

K

F

A

A

I

T

T

R

A

A

N

L

I

M

S

T

I

A

G

L

A

V

A

I
x
F

F

L

A

L

L

I

F

Y

T
x
A

A

S

I
x
Y

T

A

L

L

I

A

M

F

W

W

D

Y

A

G

V

T

Y

T

G

L

V

V

A

L

E

S

G

G

R

E

I

Y

S

S

G

I

G

G

S

Q

I

V

T

L

A

T

F

V

V

F

I
x
F

T

S

A

-

G

-

L

V

V

L

T

I

G

G

A

A

F

F

-

S

-

V

G

G

A

Q

L

A

T

S

E

P

V

S

Y

I

G

E

D

A

L

F

L

A

R

N

A

A

S

R

G

G

A

A

S

Y

R

E

L

I

A

F

E

K

L

I

A

D

Q

N

E

K

P

P

D

S

I

I

A

D

A

S

P

Y

A

S

N

K

P

S

V

G

P

H

L

K

P

P

V

D

P

N

P

I

L

K

G

G

A

N

I

L

D

E

F

F

K

R

N

N

V

V

T

H

F

F

R

S

Y

Y

P

P

T

S

R

R

P

K

E

E

V

V

S

K

A

I

L

L

H

K

Q

G

F

L

T

N

L

L

S

K

V

V

A

Q

P

S

G

G

E

Q

T

T

V

V

A

A

V

L

V

V

G

G

P

N

S

S

G

G

A

C

G

G

K

K

S

S

T

T

L

T

F

V

Q

Q

L

L

V

M

Q

Q

R

R

F

L

Y

Y

D

D

P

P

E

T

G

E

G

G

E

M

I

V

R

S

V

V

D

D

G

G

I

Q

P

D

V

I

R

R

S

T

A

I

D

N

P

V

G

R

E

F

V

L

R

R

A

E

R

I

M

I

A

G

M

V

V

V

P

S

Q

Q

E

E

T

P

V

V

I

L

F

F

A

A

A

T

T

T

A

I

R

A

D

E

N

N

L

I

R

R

Y

Y

G

G

R

R

W

E

D

N

A

V

T

T

E

M

E

D

Q

E

I

I

W

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K

A

A

A

V

E

K

A

E

A

A

N

N

A

A

A

Y

E

D

F

F

L

I

R

M

E

K

L

L

P

P

Q

H

G

K

L

F

E

D

T

T

F

L

L

V

G

G

E

E

D

R

G

G

A

A

R

Q

L

L

S

S

G

G

Q

Q

R

K

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Q

R

R

L

I

A

A

I

I

A

A

R

R

A

A

L

L

L

V

R

R

D

N

A

P

P

K

I

I

L

L

D

D

E

E

A

A

T

T

S

S

A

A

L

L

D

D

A

T

E

E

S

S

E

E

R

A

L

V

V

V

Q

Q

D

V

A

A

L

L

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D

H

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L

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G

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R

T

T

L

I

V

V

I

I

A

A

H

H

R

R

L

L

A

S

T

T

V

V

R

R

A

N

A

A

K

D

R

V

I

I

I

A

V

G

M

F

D

D

E

D

G

G

R

V

I

I

V

V

E

E

T

K

G

G

T

N

H

H

A

D

E

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L

L

V

M

A

K

K

E

G

K

G

G

L

I

Y

Y

A

F

K

K

L

L

A

V

S

T

L

M

Q

Q

binding cholesterol: A259 (≠ T269)
binding elacridar: W183 (≠ L193), L187 (≠ V197), F190 (= F200), F254 (≠ I264), Y261 (≠ I271), F287 (≠ I297)

Sites not aligning to the query:

binding cholesterol: 644, 727, 728, 757, 846, 847, 880
binding elacridar: 38, 616, 620, 658, 841, 874, 882

Query Sequence

>Ga0059261_3276 Ga0059261_3276 ABC-type multidrug transport system, ATPase and permease components
MAEPASKPETSPTPRKLSSLRIVWRHTSRYPLQLGLAIVALILAAGATLWIPRTFKEVVD
KGFGADADPSRIGAYFQGLLLVVVVLAFATAMRFYFVSWLGERTVADLRAAVHRNLLRLP
PKWFEENRPSEIASRLTADTAVVEQIVGTTVSVALRNLLIAIGGTVYLFVLAPKIAAYLI
IGIPVIVLPIMWLGGRVRRFSRTSQDRVADIGAIASETLGAMRIVQAFGQERREGERFQT
AVDAGFATARKRFATRALMTALVIFALFTAITLIMWDAVYGVAEGRISGGSITAFVITAG
LVTGAFGALTEVYGDLLRASGAASRLAELLAQEPDIAAPANPVPLPVPPLGAIDFKNVTF
RYPTRPEVSALHQFTLSVAPGETVAVVGPSGAGKSTLFQLVQRFYDPEGGEIRVDGIPVR
SADPGEVRARMAMVPQETVIFAATARDNLRYGRWDATEEQIWQAAEAANAAEFLRELPQG
LETFLGEDGARLSGGQRQRLAIARALLRDAPILLLDEATSALDAESERLVQDALEHLMQG
RTTLVIAHRLATVRAAKRIIVMDEGRIVETGTHAELVAKGGLYAKLASLQFNDAP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory