SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ga0059261_3309 FitnessBrowser__Korea:Ga0059261_3309 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P33221 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; GAR transformylase T; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Escherichia coli (strain K12) (see 5 papers)
56% identity, 98% coverage: 6:386/388 of query aligns to 14:391/392 of P33221

query
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P33221

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EL 22:23 (= EL 14:15) binding
E82 (= E74) binding
R114 (= R106) binding
K155 (= K147) binding
SSGKGQ 160:165 (= SSGKGQ 152:157) binding
G162 (= G154) mutation to I: Strong decrease in the reaction rate for the conversion of formate to FGAR and in the affinity for formate. 3- and 2-fold decrease in the affinity for ATP and GAR, respectively.
K179 (≠ D171) modified: N6-acetyllysine
EGVV 195:198 (≠ EEFV 187:190) binding
E203 (= E195) binding
E267 (= E259) binding
E279 (= E271) binding
D286 (= D278) binding
K355 (= K350) binding
RR 362:363 (= RR 357:358) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1kjiA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-amppcp (see paper)
56% identity, 98% coverage: 6:386/388 of query aligns to 13:388/389 of 1kjiA

query
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1kjiA

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active site: E114 (≠ D107), K154 (= K147), S159 (= S152), G161 (= G154), E264 (= E259), E276 (= E271), D283 (= D278), T284 (= T279), R360 (= R358)
binding phosphomethylphosphonic acid adenylate ester: R113 (= R106), S129 (= S122), I152 (≠ V145), K154 (= K147), S158 (= S151), S159 (= S152), S160 (= S153), G161 (= G154), Q164 (= Q157), E192 (= E187), G193 (≠ E188), V194 (≠ F189), V195 (= V190), F197 (= F192), E200 (= E195), Q222 (= Q217), E264 (= E259), F266 (= F261), E276 (= E271)
binding magnesium ion: E264 (= E259), E276 (= E271)

1ez1A Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg, amppnp, and gar (see paper)
56% identity, 98% coverage: 6:386/388 of query aligns to 13:388/389 of 1ez1A

query
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active site: E114 (≠ D107), K154 (= K147), S159 (= S152), G161 (= G154), E264 (= E259), E276 (= E271), D283 (= D278), T284 (= T279), R360 (= R358)
binding phosphoaminophosphonic acid-adenylate ester: R113 (= R106), S129 (= S122), I152 (≠ V145), K154 (= K147), S158 (= S151), S159 (= S152), S160 (= S153), G161 (= G154), Q164 (= Q157), E192 (= E187), G193 (≠ E188), V194 (≠ F189), V195 (= V190), F197 (= F192), E200 (= E195), Q222 (= Q217), E264 (= E259), F266 (= F261), E276 (= E271)
binding glycinamide ribonucleotide: G20 (= G13), E21 (= E14), L22 (= L15), E81 (= E74), I82 (≠ V75), S160 (= S153), D283 (= D278), K352 (= K350), R359 (= R357), R360 (= R358)
binding magnesium ion: E264 (= E259), E276 (= E271)

1eyzA Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg and amppnp (see paper)
56% identity, 98% coverage: 6:386/388 of query aligns to 13:388/389 of 1eyzA

query
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1eyzA

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C

K

Q

R

R

L

L

G

G

A

V

Q

E

V

V

I

I

A

A

C

V

D

D

S

R

Y

Y

A

A

N

D

A

A

P

P

A

A

M

M

Q

H

V

V

A

A

D

H

G

R

H

S

E

H

V

V

F

I

S

N

M

M

L

L

D

D

G

G

E

D

A

A

L

L

R

R

A

R

A

V

I

V

A

E

K

L

H

E

A

K

P

P

D

H

Y

Y

V

I

V

V

P

P

E

E

V

I

E

E

A

A

I

I

R

A

T

T

E

D

V

M

L

L

A

I

D

Q

V

L

E

E

A

E

E

E

G

G

V

L

T

N

V

V

P

P

S

C

A

A

R

R

A

A

A

T

Q

K

M

L

T

T

M

M

N

N

R
|
R

D
x
E

G

G

I

I

R

R

E

R

V

L

A

A

V

E

E

E

L

L

G

Q

L

L

R

P

T

T

S
|
S

R

T

Y

Y

R

R

Y

F

A

A

E

D

S

S

L

E

E

S

E

L

V

F

L

R

A

E

G

A

A

V

E

A

H

D

T

I

G

G

L

Y

P

P

C

C

V
x
I

I

V

K
|
K

P

P

V

V

M

M

S
|
S

G
|
G

K

K

G

G

Q
|
Q

S

T

T

F

V

I

R

R

E

S

A

A

S

E

A

Q

L

L

E

A

A

Q

A

A

W

W

D

K

Y

Y

A

A

V

-

A

-

N

-

M

Q

R

Q

G

G

D

G

R

R

A

A

-

G

R

R

V

V

I

I

V

V

E
|
E

E
x
G

F
x
V

V
|
V

D

K

F
|
F

D

D

Y

F

E
|
E

I

I

T

T

L

L

T

T

V

V

R

S

S

A

R

V

A

D

G

G

V

V

S

H

F

F

C

C

P

A

P

P

I

V

G

G

H

H

R

R

Q
|
Q

E

E

R

D

G

G

D

D

Y

Y

Q

R

E

E

S

S

W

W

Q

Q

P

P

A

Q

M

M

S

S

A

P

Q

L

A

A

I

L

A

E

D

R

A

A

Q

Q

D

E

M

I

A

A

R

R

K

K

V

V

D

L

D

A

L

L

G

G

Y

Y

G

G

I

L

F

F

G

G

V

V

E
|
E

F

L

F
|
F

V

V

K

C

G

G

E

D

E

E

V

V

I

I

F

F

S

S

E
|
E

L

V

S

S

P

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

M

M

V

V

T

T

L

L

I

I

S

S

Q

Q

N

D

L

L

S

S

E

E

F

F

D

A

L

L

H

H

A

V

R

R

A

A

V

F

L

L

G

G

L

L

P

P

V

V

P

G

E

G

V

I

R

R

L

Q

Y

Y

G

G

P

P

S

A

A

A

S

S

A

A

V

V

I

I

L

L

A

P

D

Q

R

L

D

T

G

S

E

Q

A

N

P

V

G

T

Y

F

E

D

G

N

V

V

A

Q

E

N

A

A

L

V

A

-

V

-

P

-

G

G

G

A

E

D

I

L

D

Q

L

I

R

R

I

L

F

F

A

G

K

K

P

P

T

E

T

I

R

D

P

G

Y

S

R

R

R
|
R

M

L

G

G

V

V

A

A

L

L

A

A

L

T

A

A

G

E

D

S

T

V

G

V

A

D

A

A

R

I

T

E

L

R

A

A

A

K

E

H

A

A

G

G

K

Q

V

V

R

K

I

V

N

Q

active site: E114 (≠ D107), K154 (= K147), S159 (= S152), G161 (= G154), E264 (= E259), E276 (= E271), D283 (= D278), T284 (= T279), R360 (= R358)
binding phosphoaminophosphonic acid-adenylate ester: R113 (= R106), S129 (= S122), I152 (≠ V145), K154 (= K147), S158 (= S151), S159 (= S152), S160 (= S153), G161 (= G154), Q164 (= Q157), E192 (= E187), G193 (≠ E188), V194 (≠ F189), V195 (= V190), F197 (= F192), E200 (= E195), Q222 (= Q217), E264 (= E259), F266 (= F261), E276 (= E271)
binding magnesium ion: E264 (= E259), E276 (= E271)

1kjjA Crystal structure of glycniamide ribonucleotide transformylase in complex with mg-atp-gamma-s (see paper)
55% identity, 98% coverage: 6:386/388 of query aligns to 13:385/386 of 1kjjA

query
sites
1kjjA

K

R

I

V

L

M

L

L

G

G

S

S

G

G

E

E

L

L

G

G

R

K

E

E

F

V

V

A

I

I

S

E

A

C

K

Q

R

R

L

L

G

G

A

V

Q

E

V

V

I

I

A

A

C

V

D

D

S

R

Y

Y

A

A

N

D

A

A

P

P

A

A

M

M

Q

H

V

V

A

A

D

H

G

R

H

S

E

H

V

V

F

I

S

N

M

M

L

L

D

D

G

G

E

D

A

A

L

L

R

R

A

R

A

V

I

V

A

E

K

L

H

E

A

K

P

P

D

H

Y

Y

V

I

V

V

P

P

E

E

V

I

E

E

A

A

I

I

R

A

T

T

E

D

V

M

L

L

A

I

D

Q

V

L

E

E

A

E

E

E

G

G

V

L

T

N

V

V

P

P

S

C

A

A

R

R

A

A

A

T

Q

K

M

L

T

T

M

M

N

N

R
|
R

D
x
E

G

G

I

I

R

R

E

R

V

L

A

A

V

E

E

E

L

L

G

Q

L

L

R

P

T

T

S
|
S

R

T

Y

Y

R

R

Y

F

A

A

E

D

S

S

L

E

E

S

E

L

V

F

L

R

A

E

G

A

A

V

E

A

H

D

T

I

G

G

L

Y

P

P

C

C

V
x
I

I

V

K
|
K

P

P

V

V

M

M

S
|
S

G
|
G

K

K

G

G

Q
|
Q

S

T

T

F

V

I

R

R

E

S

A

A

S

E

A

Q

L

L

E

A

A

Q

A

A

W

W

D

K

Y

Y

A

A

V

Q

A

Q

N

G

M

-

R

-

G

-

D

-

R

-

A

G

R

R

V

V

I

I

V

V

E
|
E

E
x
G

F
x
V

V
|
V

D

K

F
|
F

D

D

Y

F

E
|
E

I

I

T

T

L

L

T

T

V

V

R

S

S

A

R

V

A

D

G

G

V

V

S

H

F

F

C

C

P

A

P

P

I

V

G

G

H

H

R

R

Q
|
Q

E

E

R

D

G

G

D

D

Y

Y

Q

R

E

E

S

S

W

W

Q

Q

P

P

A

Q

M

M

S

S

A

P

Q

L

A

A

I

L

A

E

D

R

A

A

Q

Q

D

E

M

I

A

A

R

R

K

K

V

V

D

L

D

A

L

L

G

G

Y

Y

G

G

I

L

F

F

G

G

V

V

E
|
E

F

L

F
|
F

V

V

K

C

G

G

E

D

E

E

V

V

I

I

F

F

S

S

E
|
E

L

V

S

S

P

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

M

M

V

V

T

T

L

L

I

I

S

S

Q

Q

N

D

L

L

S

S

E

E

F

F

D

A

L

L

H

H

A

V

R

R

A

A

V

F

L

L

G

G

L

L

P

P

V

V

P

G

E

G

V

I

R

R

L

Q

Y

Y

G

G

P

P

S

A

A

A

S

S

A

A

V

V

I

I

L

L

A

P

D

Q

R

L

D

T

G

S

E

Q

A

N

P

V

G

T

Y

F

E

D

G

N

V

V

A

Q

E

N

A

A

L

V

A

-

V

-

P

-

G

G

G

A

E

D

I

L

D

Q

L

I

R

R

I

L

F

F

A

G

K

K

P

P

T

E

T

I

R

D

P

G

Y

S

R

R

R
|
R

M

L

G

G

V

V

A

A

L

L

A

A

L

T

A

A

G

E

D

S

T

V

G

V

A

D

A

A

R

I

T

E

L

R

A

A

A

K

E

H

A

A

G

G

K

Q

V

V

R

K

I

V

N

Q

active site: E114 (≠ D107), K154 (= K147), S159 (= S152), G161 (= G154), E261 (= E259), E273 (= E271), D280 (= D278), T281 (= T279), R357 (= R358)
binding phosphothiophosphoric acid-adenylate ester: R113 (= R106), S129 (= S122), I152 (≠ V145), K154 (= K147), S158 (= S151), S159 (= S152), S160 (= S153), G161 (= G154), Q164 (= Q157), E189 (= E187), G190 (≠ E188), V191 (≠ F189), V192 (= V190), F194 (= F192), E197 (= E195), Q219 (= Q217), E261 (= E259), F263 (= F261), E273 (= E271)
binding magnesium ion: E261 (= E259), E273 (= E271)

1kj8A Crystal structure of purt-encoded glycinamide ribonucleotide transformylase in complex with mg-atp and gar (see paper)
55% identity, 98% coverage: 6:386/388 of query aligns to 13:385/386 of 1kj8A

query
sites
1kj8A

K

R

I

V

L

M

L

L

G

G

S

S

G
|
G

E
|
E

L
|
L

G

G

R

K

E

E

F

V

V

A

I

I

S

E

A

C

K

Q

R

R

L

L

G

G

A

V

Q

E

V

V

I

I

A

A

C

V

D

D

S

R

Y

Y

A

A

N

D

A

A

P

P

A

A

M

M

Q

H

V

V

A

A

D

H

G

R

H

S

E

H

V

V

F

I

S

N

M

M

L

L

D

D

G

G

E

D

A

A

L

L

R

R

A

R

A

V

I

V

A

E

K

L

H

E

A

K

P

P

D

H

Y

Y

V

I

V

V

P

P

E
|
E

V
x
I

E

E

A

A

I

I

R

A

T

T

E

D

V

M

L

L

A

I

D

Q

V

L

E

E

A

E

E

E

G

G

V

L

T

N

V

V

P

P

S

C

A

A

R

R

A

A

A

T

Q

K

M

L

T

T

M

M

N

N

R
|
R

D
x
E

G

G

I

I

R

R

E

R

V

L

A

A

V

E

E

E

L

L

G

Q

L

L

R

P

T

T

S
|
S

R

T

Y

Y

R

R

Y

F

A

A

E

D

S

S

L

E

E

S

E

L

V

F

L

R

A

E

G

A

A

V

E

A

H

D

T

I

G

G

L

Y

P

P

C

C

V
x
I

I

V

K
|
K

P

P

V

V

M

M

S
|
S

G
|
G

K

K

G

G

Q
|
Q

S

T

T

F

V

I

R

R

E

S

A

A

S

E

A

Q

L

L

E

A

A

Q

A

A

W

W

D

K

Y

Y

A

A

V

Q

A

Q

N

G

M

-

R

-

G

-

D

-

R

-

A

G

R

R

V

V

I

I

V

V

E
|
E

E
x
G

F
x
V

V
|
V

D

K

F
|
F

D

D

Y

F

E
|
E

I

I

T

T

L

L

T

T

V

V

R

S

S

A

R

V

A

D

G

G

V

V

S

H

F

F

C

C

P

A

P

P

I

V

G

G

H

H

R

R

Q
|
Q

E

E

R

D

G
|
G

D

D

Y

Y

Q

R

E

E

S

S

W

W

Q

Q

P

P

A

Q

M

M

S

S

A

P

Q

L

A

A

I

L

A

E

D

R

A

A

Q

Q

D

E

M

I

A

A

R

R

K

K

V

V

D

L

D

A

L

L

G

G

Y

Y

G

G

I

L

F

F

G

G

V

V

E
|
E

F

L

F
|
F

V

V

K

C

G

G

E

D

E

E

V

V

I

I

F

F

S

S

E
|
E

L

V

S

S

P

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

M

M

V

V

T

T

L

L

I

I

S

S

Q

Q

N

D

L

L

S

S

E

E

F

F

D

A

L

L

H

H

A

V

R

R

A

A

V

F

L

L

G

G

L

L

P

P

V

V

P

G

E

G

V

I

R

R

L

Q

Y

Y

G

G

P

P

S

A

A

A

S

S

A

A

V

V

I

I

L

L

A

P

D

Q

R

L

D

T

G

S

E

Q

A

N

P

V

G

T

Y

F

E

D

G

N

V

V

A

Q

E

N

A

A

L

V

A

-

V

-

P

-

G

G

G

A

E

D

I

L

D

Q

L

I

R

R

I

L

F

F

A

G

K
|
K

P

P

T

E

T

I

R

D

P

G

Y

S

R
|
R

M

L

G

G

V

V

A

A

L

L

A

A

L

T

A

A

G

E

D

S

T

V

G

V

A

D

A

A

R

I

T

E

L

R

A

A

A

K

E

H

A

A

G

G

K

Q

V

V

R

K

I

V

N

Q

active site: E114 (≠ D107), K154 (= K147), S159 (= S152), G161 (= G154), E261 (= E259), E273 (= E271), D280 (= D278), T281 (= T279), R357 (= R358)
binding adenosine-5'-triphosphate: R113 (= R106), S129 (= S122), I152 (≠ V145), K154 (= K147), S158 (= S151), S159 (= S152), S160 (= S153), G161 (= G154), Q164 (= Q157), E189 (= E187), G190 (≠ E188), V191 (≠ F189), V192 (= V190), F194 (= F192), E197 (= E195), Q219 (= Q217), G222 (= G220), E261 (= E259), F263 (= F261), E273 (= E271)
binding glycinamide ribonucleotide: G20 (= G13), E21 (= E14), L22 (= L15), E81 (= E74), I82 (≠ V75), S159 (= S152), S160 (= S153), D280 (= D278), K349 (= K350), R356 (= R357), R357 (= R358)
binding magnesium ion: E261 (= E259), E273 (= E271)

1kjqA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-adp (see paper)
55% identity, 98% coverage: 6:386/388 of query aligns to 13:387/388 of 1kjqA

query
sites
1kjqA

K

R

I

V

L

M

L

L

G

G

S

S

G

G

E

E

L

L

G

G

R

K

E

E

F

V

V

A

I

I

S

E

A

C

K

Q

R

R

L

L

G

G

A

V

Q

E

V

V

I

I

A

A

C

V

D

D

S

R

Y

Y

A

A

N

D

A

A

P

P

A

A

M

M

Q

H

V

V

A

A

D

H

G

R

H

S

E

H

V

V

F

I

S

N

M

M

L

L

D

D

G

G

E

D

A

A

L

L

R

R

A

R

A

V

I

V

A

E

K

L

H

E

A

K

P

P

D

H

Y

Y

V

I

V

V

P

P

E

E

V

I

E

E

A

A

I

I

R

A

T

T

E

D

V

M

L

L

A

I

D

Q

V

L

E

E

A

E

E

E

G

G

V

L

T

N

V

V

P

P

S

C

A

A

R

R

A

A

A

T

Q

K

M

L

T

T

M

M

N

N

R
|
R

D
x
E

G

G

I

I

R

R

E

R

V

L

A

A

V

E

E

E

L

L

G

Q

L

L

R

P

T

T

S
|
S

R

T

Y

Y

R

R

Y

F

A

A

E

D

S

S

L

E

E

S

E

L

V

F

L

R

A

E

G

A

A

V

E

A

H

D

T

I

G

G

L

Y

P

P

C

C

V
x
I

I

V

K
|
K

P

P

V

V

M

M

S

S

S

-

G

-

K

K

G

G

Q
|
Q

S

T

T

F

V

I

R

R

E

S

A

A

S

E

A

Q

L

L

E

A

A

Q

A

A

W

W

D

K

Y

Y

A

A

V

Q

A

Q

N

G

M

G

R

R

G

A

D

G

R

A

A

G

R

R

V

V

I

I

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E
|
E

E
x
G

F
x
V

V
|
V

D

K

F
|
F

D

D

Y

F

E
|
E

I

I

T

T

L

L

T

T

V

V

R

S

S

A

R

V

A

D

G

G

V

V

S

H

F

F

C

C

P

A

P

P

I

V

G

G

H

H

R

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Q
|
Q

E

E

R

D

G

G

D

D

Y

Y

Q

R

E

E

S

S

W

W

Q

Q

P

P

A

Q

M

M

S

S

A

P

Q

L

A

A

I

L

A

E

D

R

A

A

Q

Q

D

E

M

I

A

A

R

R

K

K

V

V

D

L

D

A

L

L

G

G

Y

Y

G

G

I

L

F

F

G

G

V

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E
|
E

F

L

F
|
F

V

V

K

C

G

G

E

D

E

E

V

V

I

I

F

F

S

S

E
|
E

L

V

S

S

P

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

M

M

V

V

T

T

L

L

I

I

S

S

Q

Q

N

D

L

L

S

S

E

E

F

F

D

A

L

L

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H

A

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R

A

A

V

F

L

L

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G

L

L

P

P

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V

P

G

E

G

V

I

R

R

L

Q

Y

Y

G

G

P

P

S

A

A

A

S

S

A

A

V

V

I

I

L

L

A

P

D

Q

R

L

D

T

G

S

E

Q

A

N

P

V

G

T

Y

F

E

D

G

N

V

V

A

Q

E

N

A

A

L

V

A

-

V

-

P

-

G

G

G

A

E

D

I

L

D

Q

L

I

R

R

I

L

F

F

A

G

K

K

P

P

T

E

T

I

R

D

P

G

Y

S

R

R

R
|
R

M

L

G

G

V

V

A

A

L

L

A

A

L

T

A

A

G

E

D

S

T

V

G

V

A

D

A

A

R

I

T

E

L

R

A

A

A

K

E

H

A

A

G

G

K

Q

V

V

R

K

I

V

N

Q

active site: E114 (≠ D107), K154 (= K147), E263 (= E259), E275 (= E271), D282 (= D278), T283 (= T279), R359 (= R358)
binding adenosine-5'-diphosphate: R113 (= R106), S129 (= S122), I152 (≠ V145), K154 (= K147), Q161 (= Q157), E191 (= E187), G192 (≠ E188), V193 (≠ F189), V194 (= V190), F196 (= F192), E199 (= E195), Q221 (= Q217), E263 (= E259), F265 (= F261), E275 (= E271)
binding magnesium ion: E263 (= E259), E275 (= E271)

O58056 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
45% identity, 98% coverage: 6:385/388 of query aligns to 18:410/430 of O58056

query
sites
O58056

K

K

I

I

L

L

G

G

S

S

G

G

E

E

L

L

G

G

R

K

E

E

F

I

V

A

I

I

S

E

A

A

K

Q

R

R

L

L

G

G

A

V

Q

E

V

V

I

V

A

A

C

V

D

D

S

R

Y

Y

A

A

N

N

A

A

P

P

A

A

M

M

Q

Q

V

V

A

A

D

H

G

R

H

S

E

Y

V

V

F

G

S

N

M

M

L

M

D

D

G

K

E

D

A

F

L

L

R

W

A

S

A

V

I

V

A

E

K

R

H

E

A

K

P

P

D

D

Y

A

V

I

P

P

E

E

V

I

E

E

A

A

I

I

R

N

T

L

E

D

V

A

L

L

A

F

D

E

V

F

E

E

A

K

E

D

G

G

V

Y

T

F

V

V

P

P

S

N

A

A

R

R

A

A

A

T

Q

W

M

I

T

A

M

M

N

H

R
|
R

D

E

G

R

I

L

R

R

E

E

V

T

A

L

A

V

V

K

E

E

L

A

G

K

L

V

R

P

T

T

S

S

R

R

Y

Y

R

M

Y

Y

A

A

E

T

S

T

L

L

E

D

E

E

V

L

L

Y

A

E

G

A

A

C

E

E

H

K

T

I

G

G

L

Y

P

P

C

C

V

H

I

T

K
|
K

P

A

V

I

M

M

S

S

G

G

K

K

G

G

Q

S

S

Y

T

F

V

V

R

K

E

G

A

P

S

E

A

D

L

I

E

P

A

K

A

A

W

W

D

E

Y

E

A

A

V

K

A

T

N

K

M

A

R

R

G

G

D

S

R

A

A

E

R

K

V

I

I

I

V

V

E
|
E

F
x
H

V
x
I

D

D

F

F

D

D

Y

V

E
|
E

I

V

T

T

L

E

L

L

T

A

V

V

R

R

S

H

-

F

-

D

-

E

-

N

-

G

R

E

A

I

G

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V

T

S

T

F

F

C

P

P

K

P

P

I

V

G

G

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H

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Y

Q

Q

E

I

R

D

G

G

D

D

Y

Y

Q

H

E

A

S

S

W

W

Q

Q

P

P

A

A

A

E

M

I

S

S

A

E

Q

K

A

A

I

E

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R

D

E

A

V

Q

Y

D

R

M

I

A

A

R

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K

R

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I

V

T

D

D

D

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L

L

G

G

Y

L

G

G

I

I

F

F

G

G

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V

E

E

F

M

F

F

V

V

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K

G

G

E

D

E

K

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V

I

W

F

A

S

N

E

E

L

V

S

S

P

P

R

R

P

P

H

H

D

D

T

T

G

G

M

M

V

V

T

T

L

L

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A

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S

Q

H

-

P

N

G

L

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S

S

E

E

F

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D

A

L

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H

H

A

L

R

R

A

A

V

V

L

L

G

G

L

L

P

P

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I

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P

-

G

-

E

-

W

-

V

-

D

E

G

V

Y

R

R

L

L

Y

F

G

P

-

M

-

L

-

I

P

P

S

A

A

A

S

T

A

H

V

V

I

I

L

K

A

A

D

K

R

V

D

S

G

G

E

Y

A

S

P

P

G

R

Y

F

E

R

G

G

V

L

A

V

E

K

A

A

L

L

A

S

V

V

P

P

G

N

G

A

E

T

I

V

D

-

L

-

R

R

I

L

F

F

A

G

K

K

P

P

T

E

T

A

R

Y

P

V

Y

G

R

R

M

L

G

G

V

I

A

A

L

L

A

A

L

W

A

D

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K

D

D

T

V

G

E

A

V

A

A

R

K

T

R

L

K

A

A

A

E

E

M

A

V

A

A

G

H

K

M

V

I

R

E

I

L

R118 (= R106) binding
K159 (= K147) binding
EEHI 199:202 (≠ EEFV 187:190) binding
E207 (= E195) binding

2dwcB Crystal structure of probable phosphoribosylglycinamide formyl transferase from pyrococcus horikoshii ot3 complexed with adp
44% identity, 98% coverage: 6:385/388 of query aligns to 20:399/409 of 2dwcB

query
sites
2dwcB

K

K

I

I

L

L

G

G

S

S

G

G

E

E

L

L

G

G

R

K

E

E

F

I

V

A

I

I

S

E

A

A

K

Q

R

R

L

L

G

G

A

V

Q

E

V

V

I

V

A

A

C

V

D

D

S

R

Y

Y

A

A

N

N

A

A

P

P

A

A

M

M

Q

Q

V

V

A

A

D

H

G

R

H

S

E

Y

V

V

F

G

S

N

M

M

L

M

D

D

G

K

E

D

A

F

L

L

R

W

A

S

A

V

I

V

A

E

K

R

H

E

A

K

P

P

D

D

Y

A

V

I

P

P

E

E

V

I

E

E

A

A

I

I

R

N

T

L

E

D

V

A

L

L

A

F

D

E

V

F

E

E

A

K

E

D

G

G

V

Y

T

F

V

V

P

P

S

N

A

A

R

R

A

A

A

T

Q

W

M

I

T

A

M

M

N

H

R
|
R

D

E

G

R

I

L

R

R

E

E

V

T

A

L

A

V

V

K

E

E

L

A

G

K

L

V

R

P

T

T

S
|
S

R

R

Y

Y

R

M

Y

Y

A

A

E

T

S

T

L

L

E

D

E

E

V

L

L

Y

A

E

G

A

A

C

E

E

H

K

T

I

G

G

L

Y

P

P

C

C

V
x
H

I

T

K
|
K

P

A

V

I

M

M

S

S

S

-

G

-

K

-

G

G

Q

S

S

Y

T

F

V

V

R

K

E

G

A

P

S

E

A

D

L

I

E

P

A

K

A

A

W

W

D

-

Y

-

A

-

V

-

A

-

N

-

M

-

R

-

G

-

D

E

R

E

A

E

R

K

V

I

I

I

V

V

E
|
E

F
x
H

V
x
I

D

D

F
|
F

D

D

Y

V

E
|
E

I

V

T

T

L

E

L

L

T

A

V

V

R

R

S

H

-

F

-

D

-

E

-

N

-

G

R

E

A

I

G

V

V

T

S

T

F

F

C

P

P

K

P

P

I

V

G

G

H

H

R

Y

Q
|
Q

E

I

R

D

G

G

D

D

Y

Y

Q

H

E

A

S

S

W

W

Q

Q

P

P

A

A

A

E

M

I

S

S

A

E

Q

K

A

A

I

E

A

R

D

E

A

V

Q

Y

D

R

M

I

A

A

R

K

K

R

V

I

V

T

D

D

D

V

L

L

G

G

Y

L

G

G

I

I

F

F

G

G

V

V

E
|
E

F

M

F
|
F

V

V

K

K

G

G

E

D

E

K

V

V

I

W

F

A

S
x
N

E
|
E

L

V

S

S

P

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

M

M

V

V

T

T

L

L

I

A

S

S

Q

H

-

P

N

G

L

F

S

S

E

E

F

F

D

A

L

L

H

H

A

L

R

R

A

A

V

V

L

L

G

G

L

L

P

P

V

I

P

P

-

G

-

E

-

W

-

V

-

D

E

G

V

Y

R

R

L

L

Y

F

G

P

-

M

-

L

-

I

P

P

S

A

A

A

S

T

A

H

V

V

I

I

L

K

A

A

D

K

R

V

D

S

G

G

E

Y

A

S

P

P

G

R

Y

F

E

R

G

G

V

L

A

V

E

K

A

A

L

L

A

S

V

V

P

P

G

N

G

A

E

T

I

V

D

-

L

-

R

R

I

L

F

F

A

G

K

K

P

P

T

E

T

A

R

Y

P

V

Y

G

R

R

R
|
R

M

L

G

G

V

I

A

A

L

L

A

A

L

W

A

D

G

K

D

D

T

V

G

E

A

V

A

A

R

K

T

R

L

K

A

A

A

E

E

M

A

V

A

A

G

H

K

M

V

I

R

E

I

L

active site: E265 (= E259), E277 (= E271), D284 (= D278), T285 (= T279), R372 (= R358)
binding adenosine-5'-diphosphate: R120 (= R106), S136 (= S122), H159 (≠ V145), K161 (= K147), E188 (= E187), E189 (= E188), H190 (≠ F189), I191 (≠ V190), F193 (= F192), E196 (= E195), Q223 (= Q217), F267 (= F261), N276 (≠ S270), E277 (= E271)

3ax6A Crystal structure of n5-carboxyaminoimidazole ribonucleotide synthetase from thermotoga maritima
28% identity, 88% coverage: 21:360/388 of query aligns to 18:332/360 of 3ax6A

query
sites
3ax6A

I

L

S

E

A

A

K

K

R

K

L

M

G

G

A

F

Q

Y

V

V

I

I

A

V

C

L

D

D

S

P

Y

T

A

P

N

R

A

S

P

P

A

A

M

G

Q

Q

V

V

A

A

D

D

G

E

H

Q

E

I

V

V

F

A

S

G

M

F

L

F

D

D

G

S

E

E

A

R

L

I

R

E

A

D

A

L

I

V

A

K

K

-

H

-

A

G

P

S

D

D

Y

V

V

T

P

Y

E

D

V

L

E

E

A

H

I

I

R

D

T

V

E

Q

V

T

L

L

A

K

D

K

V

L

E

Y

A

N

E

E

G

G

V

Y

T

K

V

I

V

H

P

P

S

S

A

P

R

Y

A

T

A

L

Q

E

M

I

T

I

M

Q

N

D

R
x
K

D

F

G

V

I

Q

R

K

E

E

V

F

A

L

A

K

V

-

E

K

L

N

G

G

L

I

R

P

T

V

S

P

R

E

Y

Y

R

K

Y

L

A

V

E

K

S

D

L

L

E

E

E

-

V

-

L

-

A

S

G

D

A

V

E

R

H

E

T

F

G

G

L

F

P

P

C

V

V
|
V

I

Q

K
|
K

P

-

V

-

M

-

S

-

S

A

S

R

G

K

K

G

G

G

Q
x
V

S

F

T

I

V

I

R

K

E

N

A

E

S

K

A

D

L

L

E

E

A

N

A

A

W

I

D

-

Y

-

A

-

V

-

A

-

N

-

M

-

R

-

G

-

D

-

R

K

A

G

R

E

V

T

I

Y

V

L

E
|
E

F
|
F

V
|
V

D

E

F
x
I

D

E

Y

K

E
|
E

I

L

T

A

L

V

L

M

T

V

V

A

R

R

S

N

R

E

A

K

G

G

V

E

S

I

F

A

C

C

P

Y

P

P

I

V

G

V

H

E

R

M

Q

-

E

-

R

-

G

-

D

-

Y

Y

Q

D

E

T

S

V

W

I

Q

A

P

P

A

A

A

R

M

I

S

E

A

E

Q

K

A

Y

I

S

A

K

D

I

A

A

Q

R

D

E

M

I

A

A

R

T

K

S

V

V

D

E

D

A

L

L

G

E

G

G

Y

V

G

G

I

I

F

F

G

G

V

I

E
|
E

F

M

F
|
F

V

L

-

T

K

K

G

Q

E

G

E

E

V

I

I

L

F

V

S
x
N

E
|
E

L

I

S

A

P

P

R

R

P

P

H

H

D
x
N

T
x
S

G

G

M

H

V

Y

T

T

L

I

I

E

S

A

Q

C

N

V

L

T

S

S

E

Q

F

F

D

E

L

Q

H

H

A

I

R

R

A

A

V

I

L

M

G

N

L

L

P

P

V

L

P

G

E

S

V

T

R

E

L

L

Y

L

G

I

P

P

S

A

A

V

S

M

A

V

V

N

I

L

L

L

A

G

D

E

R

E

D

G

G

Y

E

Y

-

G

A

K

P

P

G

A

Y

L

E

I

G

G

V

L

A

E

E

E

A

A

L

L

A

A

V

I

P

E

G

G

G

-

E

-

I

L

D

S

L

L

R

H

I

F

F

Y

A

G

K

K

P

K

T

E

T

T

R

R

P

P

Y

Y

R

R

R
x
K

M

M

G

G

active site: E231 (= E259), E244 (= E271), N251 (≠ D278), S252 (≠ T279), K330 (≠ R358)
binding adenosine-5'-diphosphate: K101 (≠ R106), V136 (= V145), K138 (= K147), V144 (≠ Q157), E164 (= E187), E165 (= E188), F166 (= F189), V167 (= V190), I169 (≠ F192), E172 (= E195), F233 (= F261), N243 (≠ S270)

3k5hA Crystal structure of carboxyaminoimidazole ribonucleotide synthase from asperigillus clavatus complexed with atp (see paper)
30% identity, 89% coverage: 17:360/388 of query aligns to 17:355/382 of 3k5hA

query
sites
3k5hA

R

R

E

M

F

L

V

V

I

E

S

S

A

A

K

N

R

R

L

L

G

N

A

I

Q

Q

V

V

I

N

A

V

C

L

D

D

S

A

Y

-

A

D

N

N

A

S

P

P

A

A

M

K

Q

Q

V

I

A

S

-

A

-

H

D

D

G

G

H

H

E

V

V

T

F

G

S

S

M

F

L

K

D

E

G

R

E

E

A

A

L

V

R

R

A

Q

A

-

I

L

A

A

K

K

H

T

A

C

P

-

D

D

Y

V

V

V

V

T

P

A

E

E

V

I

E

E

A

H

I

V

R

D

T

T

E

Y

V

A

L

L

A

E

D

E

V

V

E

A

A

S

E

E

G

-

V

V

T

K

V

I

V

E

P

P

S

S

A

W

R

Q

A

A

A

I

Q

R

M

T

T

I

M

Q

N

N

R
x
K

D

F

G

N

I

Q

R

K

E

E

V

-

A

H

A

L

V

R

E

K

L

Y

G

G

L

I

R

P

T

M

S

A

R

E

Y

H

R

R

-

E

-

L

Y

V

A

E

E

N

S

T

L

P

E

A

E

E

V

L

L

A

A

K

G

V

A

G

E

E

H

Q

T

L

G

G

L

Y

P

P

C

L

V
x
M

I

L

K
|
K

-

S

P

K

V

T

M

M

S
x
A

S
x
Y

S
x
D

G
|
G

K

R

G

G

Q
x
N

S

F

T

R

V

V

R

N

E

S

A

Q

S

D

-

D

-

I

-

P

-

E

A

A

L

L

E

E

A

A

A

L

W

K

D

D

Y

R

A

P

V

L

A

-

N

-

M

-

R

-

G

-

D

-

R

-

A

-

R

-

V

-

I

Y

V

A

E
|
E

E
x
K

F
x
W

V
x
A

D

Y

F
|
F

D

K

Y

M

E
|
E

I

L

T

A

L

V

L

I

T

V

V

V

R

K

S

T

R

K

A

D

G

E

V

V

S

L

F

S

C

Y

P

P

P

T

I

V

G

E

H

T

R

V

Q
|
Q

E

E

R

D

G
x
S

D

I

Y

C

Q

K

E

L

S

V

W

Y

Q

A

P

P

A

A

A

R

M

N

S

V

A

S

Q

D

A

A

I

I

-

N

A

Q

D

K

A

A

Q

Q

D

E

M

L

A

A

R

R

K

K

V

A

V

V

D

A

L

F

G

D

G

G

Y

K

G

G

I

V

F

F

G

G

V

V

E
|
E

-

M

F
|
F

F

L

V

L

K

E

G

D

E

D

E

S

V

I

I

M

F

L

S
x
C

E
|
E

L

I

S

A

P

S

R

R

P

I

H

H

D
x
N

T
x
S

G

G

M

H

V

Y

T

T

L

I

I

E

S

G

Q

C

N

A

L

L

S

S

E

Q

F

F

D

D

L

A

H

H

A

L

R

R

A

A

V

I

L

L

G

D

L

L

P

P

V

I

P

P

-

A

-

Q

E

S

V

L

R

E

L

I

Y

R

G

Q

P

P

S

S

A

I

S

M

A

L

V

N

I

I

L

I

A

G

D

-

R

-

D

-

G

G

E

A

A

A

P

P

G

D

-

T

-

H

Y

L

E

Q

G

A

V

A

A

E

E

C

A

A

L

L

A

S

V

I

P

P

G

N

G

A

E

S

I

I

D

H

L

L

R

-

I

-

F

Y

A

S

K

K

P

G

T

A

R

K

P

P

Y

G

R

R

R
x
K

M

M

G

G

active site: Y152 (≠ S152), G154 (= G154), E254 (= E259), E267 (= E271), N274 (≠ D278), S275 (≠ T279), K353 (≠ R358)
binding adenosine-5'-triphosphate: K104 (≠ R106), M144 (≠ V145), K146 (= K147), A151 (≠ S151), Y152 (≠ S152), D153 (≠ S153), G154 (= G154), N157 (≠ Q157), E181 (= E187), K182 (≠ E188), W183 (≠ F189), A184 (≠ V190), F186 (= F192), E189 (= E195), Q211 (= Q217), S214 (≠ G220), E254 (= E259), F256 (= F260), C266 (≠ S270), E267 (= E271)
binding magnesium ion: E254 (= E259), E267 (= E271)

3k5iA Crystal structure of n5-carboxyaminoimidazole synthase from aspergillus clavatus in complex with adp and 5-aminoimadazole ribonucleotide (see paper)
30% identity, 89% coverage: 17:360/388 of query aligns to 17:355/381 of 3k5iA

query
sites
3k5iA

R

R

E

M

F

L

V

V

I

E

S

S

A

A

K

N

R

R

L

L

G

N

A

I

Q

Q

V

V

I

N

A

V

C

L

D

D

S

A

Y

-

A

D

N

N

A

S

P

P

A

A

M

K

Q

Q

V

I

A

S

-

A

-

H

D

D

G

G

H

H

E

V

V

T

F

G

S

S

M

F

L

K

D

E

G

R

E

E

A

A