SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ga0059261_3322 Ga0059261_3322 Arabinose efflux permease to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
27% identity, 51% coverage: 91:319/445 of query aligns to 92:315/444 of Q8NLB7

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Q8NLB7

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R103 (= R102) mutation to A: Loss of transport activity.
W309 (≠ T313) mutation to V: Loss of transport activity.
D312 (= D316) mutation to A: Loss of transport activity.
R313 (= R317) mutation to A: Loss of transport activity.

Sites not aligning to the query:

54 D→A: Loss of transport activity.; D→E: Retains 50% of its transport activity.
57 D→A: Loss of transport activity.; D→E: Retains 50% of its transport activity.
317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

6rw3A The molecular basis for sugar import in malaria parasites. (see paper)
26% identity, 42% coverage: 66:252/445 of query aligns to 37:213/437 of 6rw3A

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binding beta-D-glucopyranose: Q138 (vs. gap)
binding alpha-D-glucopyranose: Q138 (vs. gap)

Sites not aligning to the query:

binding beta-D-glucopyranose: 264, 270, 357, 366
binding alpha-D-glucopyranose: 264, 269, 270, 357, 366

8sc6A Human oct1 bound to thiamine in inward-open conformation (see paper)
31% identity, 20% coverage: 84:171/445 of query aligns to 139:215/447 of 8sc6A

query
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8sc6A

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Sites not aligning to the query:

binding 3-(4-amino-2-methyl-pyrimidin-5-ylmethyl)-5-(2-hydroxy-ethyl)-4-methyl-thiazol-3-ium: 223, 226, 293, 294, 318, 378, 379

Query Sequence

>Ga0059261_3322 Ga0059261_3322 Arabinose efflux permease
MARIPGMIGVPADGDAPLDTRPAHAVPLDRRTRARAAVAGSVGNLIEWYDFYAYAYTALY
FASAFFPAGDRTAQLLNVAAIYAAGFLIRPLGGWFFGRYADRHGRRAAMIASVVLMGAGS
LLVGVLPTYATIGAAAPALLLVARLMQGFSTGGQYGAAATYLSEIAEPGKRGFYASFQFV
TLIGGQLFALLVVFALQSTMSETAIREWGWRLPFLLGAVLAGVFILFRDVMHETAEPASK
GEDAGSLKALFQHPRAMFVVMALSAAGAVTLYTFTTYMQKYLVNTAGMDVASASRTMLIV
TFAFLLLQPVLGTLSDRIGRRTNLLIFSGGMTLFAVPLLGAIGQAQTMWSAGLLVFAALA
IMSFYTSVSGLFKAELFPARVRALGVGLGHAIASAIFGGTAEWAALMLKQMGHEGLFAWY
VSAVCAVAFVVAWQMREPRAHGHLR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory