SitesBLAST
Comparing Ga0059261_3677 Ga0059261_3677 methylmalonic acid semialdehyde dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4iymC Crystal structure of putative methylmalonate-semialdehyde dehydrogenase from sinorhizobium meliloti 1021 complexed with NAD, target 011934
65% identity, 97% coverage: 7:492/503 of query aligns to 6:491/491 of 4iymC
- active site: N153 (= N154), K176 (= K177), F250 (≠ M251), C284 (= C285), E386 (= E387), Q466 (= Q467)
- binding nicotinamide-adenine-dinucleotide: I149 (= I150), T150 (= T151), P151 (= P152), F152 (= F153), N153 (= N154), F154 (= F155), K176 (= K177), K209 (= K210), V212 (= V213), F226 (= F227), V227 (= V228), G228 (= G229), S229 (= S230), I232 (= I233), G251 (= G252), C284 (= C285), E386 (= E387), F388 (= F389)
4zz7A Crystal structure of methylmalonate-semialdehyde dehydrogenase (dddc) from oceanimonas doudoroffii (see paper)
56% identity, 95% coverage: 8:487/503 of query aligns to 4:482/489 of 4zz7A
- active site: N149 (= N154), K172 (= K177), L246 (≠ M251), C280 (= C285), E382 (= E387), A462 (≠ Q467)
- binding nicotinamide-adenine-dinucleotide: T146 (= T151), P147 (= P152), F148 (= F153), N149 (= N154), K172 (= K177), E175 (= E180), K205 (= K210), V208 (= V213), F222 (= F227), V223 (= V228), G224 (= G229), S225 (= S230), I228 (= I233), L246 (≠ M251), G247 (= G252), C280 (= C285), E382 (= E387), F384 (= F389)
5tjrD X-ray crystal structure of a methylmalonate semialdehyde dehydrogenase from pseudomonas sp. Aac (see paper)
55% identity, 95% coverage: 8:487/503 of query aligns to 3:456/468 of 5tjrD
- active site: N144 (= N154), K167 (= K177), L241 (≠ M251), C270 (= C285), E356 (= E387), A436 (≠ Q467)
- binding adenosine-5'-diphosphate: I140 (= I150), T141 (= T151), F143 (= F153), K167 (= K177), E170 (= E180), K200 (= K210), F217 (= F227), S220 (= S230), I223 (= I233)
P42412 Malonate-semialdehyde dehydrogenase; MSA dehydrogenase; Methylmalonate-semialdehyde dehydrogenase; MMSA dehydrogenase; MMSDH; MSDH; EC 1.2.1.27 from Bacillus subtilis (strain 168) (see 3 papers)
43% identity, 97% coverage: 5:492/503 of query aligns to 4:485/487 of P42412
- C36 (≠ A37) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-160; A-287; A-351 and A-413.
- R107 (= R108) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- A150 (≠ T151) binding
- F152 (= F153) binding
- C160 (≠ M161) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-287; A-351 and A-413.
- K176 (= K177) binding
- E179 (= E180) binding
- R180 (= R181) binding
- S229 (= S230) binding
- T251 (≠ G252) binding
- R283 (= R284) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- C287 (≠ L288) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-351 and A-413.
- C351 (≠ V353) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-413.
- E382 (= E387) binding
- C413 (≠ A418) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-351.
1t90A Crystal structure of methylmalonate semialdehyde dehydrogenase from bacillus subtilis
43% identity, 97% coverage: 5:492/503 of query aligns to 2:483/484 of 1t90A
- active site: N151 (= N154), K174 (= K177), L248 (≠ M251), C282 (= C285), E380 (= E387), A460 (≠ Q467)
- binding nicotinamide-adenine-dinucleotide: I147 (= I150), A148 (≠ T151), P149 (= P152), F150 (= F153), N151 (= N154), W159 (= W162), K174 (= K177), E177 (= E180), R178 (= R181), H207 (≠ K210), V225 (= V228), G226 (= G229), S227 (= S230), V230 (≠ I233), L248 (≠ M251), T249 (≠ G252), C282 (= C285), E380 (= E387), F382 (= F389)
Q9HTJ1 NAD/NADP-dependent betaine aldehyde dehydrogenase; BADH; EC 1.2.1.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
31% identity, 94% coverage: 11:482/503 of query aligns to 10:479/490 of Q9HTJ1
- GAWN 150:153 (≠ TPFN 151:154) binding
- K162 (≠ M163) active site, Charge relay system
- KPSE 176:179 (= KPSE 177:180) binding
- G209 (vs. gap) binding
- GTST 230:233 (≠ SSDI 230:233) binding
- E252 (≠ M251) active site, Proton acceptor
- C286 (= C285) binding covalent; modified: Cysteine sulfenic acid (-SOH)
- E387 (= E387) binding
- E464 (≠ T465) active site, Charge relay system
4cazA Crystal structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa in complex with nadh
31% identity, 94% coverage: 11:482/503 of query aligns to 9:478/489 of 4cazA
- active site: N152 (= N154), K175 (= K177), E251 (≠ M251), C285 (= C285), E386 (= E387), E463 (≠ T465)
- binding [[(2R,3S,4R,5R)-5-[(3R)-3-aminocarbonyl-3,4-dihydro-2H-pyridin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanidyl-phosphoryl] [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl phosphate: I148 (= I150), G149 (≠ T151), W151 (≠ F153), N152 (= N154), K175 (= K177), E178 (= E180), G208 (vs. gap), G212 (≠ V213), F226 (= F227), T227 (≠ V228), G228 (= G229), G229 (≠ S230), T232 (≠ I233), V236 (= V237), E251 (≠ M251), L252 (≠ G252), C285 (= C285), E386 (= E387), F388 (= F389)
2woxA Betaine aldehyde dehydrogenase from pseudomonas aeruginosa with NAD(p) h-catalytic thiol adduct. (see paper)
31% identity, 94% coverage: 11:482/503 of query aligns to 9:478/489 of 2woxA
- active site: N152 (= N154), K175 (= K177), E251 (≠ M251), C285 (= C285), E386 (= E387), E463 (≠ T465)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I148 (= I150), G149 (≠ T151), W151 (≠ F153), N152 (= N154), K175 (= K177), S177 (= S179), E178 (= E180), G208 (vs. gap), G212 (≠ V213), F226 (= F227), T227 (≠ V228), G228 (= G229), G229 (≠ S230), T232 (≠ I233), V236 (= V237), E251 (≠ M251), L252 (≠ G252), C285 (= C285), E386 (= E387), F388 (= F389)
2wmeA Crystallographic structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa (see paper)
31% identity, 94% coverage: 11:482/503 of query aligns to 9:478/489 of 2wmeA
- active site: N152 (= N154), K175 (= K177), E251 (≠ M251), C285 (= C285), E386 (= E387), E463 (≠ T465)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G149 (≠ T151), W151 (≠ F153), K175 (= K177), S177 (= S179), E178 (= E180), G208 (vs. gap), G212 (≠ V213), F226 (= F227), G228 (= G229), G229 (≠ S230), T232 (≠ I233), V236 (= V237)
6wsbA Crystal structure of a betaine aldehyde dehydrogenase from burkholderia pseudomallei bound to cofactor NAD (see paper)
34% identity, 94% coverage: 11:482/503 of query aligns to 9:477/489 of 6wsbA
- active site: N152 (= N154), E250 (≠ M251), C284 (= C285), E462 (≠ T465)
- binding nicotinamide-adenine-dinucleotide: I148 (= I150), G149 (≠ T151), A150 (≠ P152), W151 (≠ F153), N152 (= N154), K175 (= K177), E178 (= E180), G208 (≠ K210), G211 (≠ V213), A212 (≠ D214), F225 (= F227), T226 (≠ V228), G227 (= G229), G228 (≠ S230), T231 (≠ I233), V235 (= V237), E250 (≠ M251), L251 (≠ G252), G252 (= G253), C284 (= C285), E385 (= E387), F387 (= F389)
4o6rA Crystal structure of a putative aldehyde dehydrogenase from burkholderia cenocepacia
31% identity, 91% coverage: 26:482/503 of query aligns to 22:475/489 of 4o6rA
- active site: N150 (= N154), K173 (= K177), E248 (≠ M251), C282 (= C285), E383 (= E387), E460 (≠ Q467)
- binding adenosine monophosphate: I146 (= I150), V147 (≠ T151), K173 (= K177), G206 (≠ K210), G210 (≠ D214), Q211 (≠ A215), F224 (= F227), G226 (= G229), S227 (= S230), T230 (≠ I233), R233 (≠ Y236)
8skfA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (lattice translocation disorder)
32% identity, 94% coverage: 11:482/503 of query aligns to 17:486/497 of 8skfA
- binding calcium ion: T33 (≠ V27), I34 (≠ F28), D100 (≠ E94), V187 (≠ R181)
- binding nicotinamide-adenine-dinucleotide: I156 (= I150), G157 (≠ T151), A158 (≠ P152), W159 (≠ F153), K183 (= K177), E186 (= E180), G216 (≠ D209), G220 (≠ V213), T235 (≠ V228), G236 (= G229), G237 (= G241), S240 (≠ A244), K243 (≠ R247), E259 (vs. gap), C293 (= C285), F396 (= F389)
8vr1A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (ctp bound)
32% identity, 94% coverage: 11:482/503 of query aligns to 8:477/488 of 8vr1A
8vr0A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (gmp bound)
32% identity, 94% coverage: 11:482/503 of query aligns to 8:477/488 of 8vr0A
8vqzA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (cmp bound)
32% identity, 94% coverage: 11:482/503 of query aligns to 8:477/488 of 8vqzA
8vqwC Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (coa bound)
32% identity, 94% coverage: 11:482/503 of query aligns to 8:477/488 of 8vqwC
- binding coenzyme a: I147 (= I150), W150 (≠ F153), K174 (= K177), S176 (= S179), E177 (= E180), G207 (≠ D209), G211 (≠ V213), F225 (= F227), G227 (= G229), G228 (= G241), S231 (≠ A244), H331 (= H333), F387 (= F389)
8vj3A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (fad bound)
32% identity, 94% coverage: 11:482/503 of query aligns to 8:477/488 of 8vj3A
8uzoA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (adp bound)
32% identity, 94% coverage: 11:482/503 of query aligns to 8:477/488 of 8uzoA
8uznA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (amp bound)
32% identity, 94% coverage: 11:482/503 of query aligns to 8:477/488 of 8uznA
- binding adenosine monophosphate: I147 (= I150), G148 (≠ T151), K174 (= K177), S176 (= S179), E177 (= E180), G207 (≠ D209), G211 (≠ V213), F225 (= F227), G228 (= G241), S231 (≠ A244), K234 (≠ R247)
8uzmA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (NADPH bound)
32% identity, 94% coverage: 11:482/503 of query aligns to 8:477/488 of 8uzmA
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G148 (≠ T151), W150 (≠ F153), K174 (= K177), S176 (= S179), E177 (= E180), G207 (≠ D209), G211 (≠ V213), F225 (= F227), T226 (≠ V228), G227 (= G229), G228 (= G241), S231 (≠ A244), E250 (vs. gap), G252 (= G253), C284 (= C285), E385 (= E387), F387 (= F389)
Query Sequence
>Ga0059261_3677 Ga0059261_3677 methylmalonic acid semialdehyde dehydrogenase
MATAVREISHFIGNAATAGTGTRRSDVFDPNTGAVQAQVTLGAQADLDAAMANAVRAQIS
WAATNPQRRARVMFNFKALIEKNMEELAHLLSSEHGKVIADAKGDIQRGLEVIEFVCGIP
HVLKGEYTQGAGPGIDVYSMRQPLGVVAGITPFNFPAMIPMWMFGVAIACGNAFILKPSE
RDPSVPVRLAELMLEAGLPEGVLQVVQGDKEMVDAILDHPEIKAVSFVGSSDIAHYVYRR
GVAAGKRVQAMGGAKNHGIVMPDADLDQVVNDLAGAAFGSAGERCMALPVVVPVGDKTAD
ALRAKLLPAIEALRVGVSTDAGAHYGPVVTAAHKAKIESYIQMGVDEGAELVVDGRGFTL
QGHEQGFFVGPTLFDRVTPQMQSYQEEIFGPVLQIVRAPDFETALRLPSEHQYGNGVAIF
TRNGHAAREFAARVNVGMVGINVPIPVPVAYHTFGGWKRSAFGDTNQHGMEGIKFFTKVK
TVTQRWPDGSPDGGNAFVIPTMG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory