SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ga0059261_3677 Ga0059261_3677 methylmalonic acid semialdehyde dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4iymC Crystal structure of putative methylmalonate-semialdehyde dehydrogenase from sinorhizobium meliloti 1021 complexed with NAD, target 011934
65% identity, 97% coverage: 7:492/503 of query aligns to 6:491/491 of 4iymC

query
sites
4iymC

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active site: N153 (= N154), K176 (= K177), F250 (≠ M251), C284 (= C285), E386 (= E387), Q466 (= Q467)
binding nicotinamide-adenine-dinucleotide: I149 (= I150), T150 (= T151), P151 (= P152), F152 (= F153), N153 (= N154), F154 (= F155), K176 (= K177), P177 (= P178), S178 (= S179), E179 (= E180), K209 (= K210), V212 (= V213), F226 (= F227), V227 (= V228), G228 (= G229), S229 (= S230), I232 (= I233), G251 (= G252), G252 (= G253), R283 (= R284), C284 (= C285), E386 (= E387), F388 (= F389)

4zz7A Crystal structure of methylmalonate-semialdehyde dehydrogenase (dddc) from oceanimonas doudoroffii (see paper)
56% identity, 95% coverage: 8:487/503 of query aligns to 4:482/489 of 4zz7A

query
sites
4zz7A

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active site: N149 (= N154), K172 (= K177), L246 (≠ M251), C280 (= C285), E382 (= E387), A462 (≠ Q467)
binding nicotinamide-adenine-dinucleotide: I145 (= I150), T146 (= T151), P147 (= P152), F148 (= F153), N149 (= N154), W157 (= W162), K172 (= K177), P173 (= P178), S174 (= S179), E175 (= E180), R176 (= R181), K205 (= K210), V208 (= V213), F222 (= F227), V223 (= V228), G224 (= G229), S225 (= S230), I228 (= I233), L246 (≠ M251), G247 (= G252), G248 (= G253), C280 (= C285), E382 (= E387), F384 (= F389), N410 (= N415), F449 (= F454)

5tjrD X-ray crystal structure of a methylmalonate semialdehyde dehydrogenase from pseudomonas sp. Aac (see paper)
55% identity, 95% coverage: 8:487/503 of query aligns to 3:456/468 of 5tjrD

query
sites
5tjrD

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active site: N144 (= N154), K167 (= K177), L241 (≠ M251), C270 (= C285), E356 (= E387), A436 (≠ Q467)
binding adenosine-5'-diphosphate: I140 (= I150), T141 (= T151), P142 (= P152), F143 (= F153), K167 (= K177), P168 (= P178), S169 (= S179), E170 (= E180), R171 (= R181), K200 (= K210), V203 (= V213), F217 (= F227), G219 (= G229), S220 (= S230), I223 (= I233)

P42412 Malonate-semialdehyde dehydrogenase; MSA dehydrogenase; Methylmalonate-semialdehyde dehydrogenase; MMSA dehydrogenase; MMSDH; MSDH; EC 1.2.1.-; EC 1.2.1.27 from Bacillus subtilis (strain 168) (see 3 papers)
43% identity, 97% coverage: 5:492/503 of query aligns to 4:485/487 of P42412

query
sites
P42412

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C36 (≠ A37) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-160; A-287; A-351 and A-413.
R107 (= R108) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
C160 (≠ M161) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-287; A-351 and A-413.
KPSER 176:180 (= KPSER 177:181) binding
R283 (= R284) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
C287 (≠ L288) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-351 and A-413.
C351 (≠ V353) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-413.
E382 (= E387) binding
C413 (≠ A418) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-351.

1t90A Crystal structure of methylmalonate semialdehyde dehydrogenase from bacillus subtilis
43% identity, 97% coverage: 5:492/503 of query aligns to 2:483/484 of 1t90A

query
sites
1t90A

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active site: N151 (= N154), K174 (= K177), L248 (≠ M251), C282 (= C285), E380 (= E387), A460 (≠ Q467)
binding nicotinamide-adenine-dinucleotide: I147 (= I150), A148 (≠ T151), P149 (= P152), F150 (= F153), N151 (= N154), W159 (= W162), K174 (= K177), P175 (= P178), S176 (= S179), E177 (= E180), R178 (= R181), H207 (≠ K210), V210 (= V213), F224 (= F227), V225 (= V228), G226 (= G229), S227 (= S230), V230 (≠ I233), L248 (≠ M251), T249 (≠ G252), G250 (= G253), C282 (= C285), E380 (= E387), F382 (= F389), N408 (= N415), F447 (= F454)

Q9HTJ1 NAD/NADP-dependent betaine aldehyde dehydrogenase; BADH; EC 1.2.1.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
31% identity, 94% coverage: 11:482/503 of query aligns to 10:479/490 of Q9HTJ1

query
sites
Q9HTJ1

F

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A

E

G

A

T

S

G

S

T

G

R

A

R

T

S

F

D

E

V

T

F

I

D

N

P

P

N

A

T

N

G

G

A

E

V

V

Q

L

A

A

Q

K

V

V

T

Q

L

R

G

A

A

S

Q

R

A

E

D

D

L

V

D

E

A

R

A

A

M

V

A

Q

N

S

A

A

V

V

R

E

A

G

Q

Q

I

K

S

V

W

W

A

A

T

M

N

T

P

A

Q

M

R

Q

R

R

A

S

R

R

V

I

M

L

F

R

N

R

F

A

K

V

A

D

L

I

I

L

E

R

K

E

N

R

M

N

E

D

E

E

L

L

A

A

H

A

L

L

L

E

S

T

S

L

E

D

H

T

G

G

K

K

V

P

I

L

A

A

D

E

A

T

K

R

G

S

-

V

D

D

I

I

Q

V

R

T

G

G

L

A

E

D

V

V

I

L

E

E

F

Y

V

Y

C

A

G

G

I

L

P

V

H

P

V

A

L

I

K

E

G

G

E

E

Y

Q

T

I

Q

P

G

L

A

R

G

E

P

T

G

S

I

F

D

-

V

V

Y

Y

S

T

M

R

R

R

Q

E

P

P

L

L

G

G

V

V

A

A

G

G

I

I

T
x
G

P
x
A

F
x
W

N
|
N

F

Y

P

P

A

V

M

Q

I

I

P

A

M

L

W

W

M
x
K

F

S

G

A

V

P

A

A

I

L

A

A

C

A

G

G

N

N

A

A

F

M

I

I

L

F

K
|
K

P
|
P

S
|
S

E
|
E

R

V

D

T

P

P

S

L

V

T

P

A

V

L

R

K

L

L

A

A

E

E

L

I

M

Y

L

T

E

E

A

A

G

G

L

V

P

P

E

D

G

G

V

V

L

F

Q

N

V

V

V

L

Q

T

G

G

D

S

-
x
G

K

R

E

E

M

V

V

G

D

Q

A

W

I

L

L

T

D

E

H

H

P

P

E

L

I

I

K

E

A

K

V

I

S

S

F

F

V

T

G

G

S
x
G

S
x
T

D
x
S

I
x
T

A

G

H

K

Y

K

V

V

Y

M

R

A

R

S

G

A

V

S

A

S

G

S

-

L

K

K

R

E

V

V

Q

T

A

M

M
x
E

G

L

G

G

A

G

K

K

N

S

H

P

G

L

I

I

V

I

M

F

P

P

D

D

A

A

D

D

L

L

D

D

Q

R

V

A

N

D

D

I

L

A

A

V

G

M

A

A

A

N

F

F

G

F

S

S

A

S

G

G

E

Q

R

V

C
|
C

M

T

A

N

L

G

P

T

V

R

V

V

V

F

P

I

V

H

G

R

D

S

K

Q

T

Q

A

A

D

R

A

-

L

F

R

E

A

A

K

K

L

V

L

L

P

E

A

R

I

V

E

Q

A

R

L

I

R

R

V

L

G

G

V

D

S

P

T

Q

D

D

A

E

G

N

A

T

H

N

Y

F

G

G

P

P

V

L

V

V

T

S

A

F

A

P

H

H

K

M

A

E

K

S

I

V

E

L

S

G

Y

Y

I

I

Q

E

M

S

G

G

V

K

D

A

E

Q

G

K

A

A

E

R

L

L

V

L

V

C

D

G

G

G

R

E

G

R

F

V

T

T

L

D

Q

G

G

A

H

F

E

G

Q

K

G

G

F

A

F

Y

V

V

G

A

P

P

T

T

L

V

F

F

D

T

R

D

V

C

T

R

P

D

Q

D

M

M

Q

T

S

I

Y

V

Q

R

E

E

E
|
E

I

I

F

F

G

G

P

P

V

V

L

M

Q

S

I

I

V

L

R

V

A

Y

P

D

D

D

F

E

E

D

T

E

A

A

L

I

R

R

L

R

P

A

S

N

E

D

H

T

Q

E

Y

Y

G

G

N

L

G

A

V

A

A

G

I

V

F

V

T

T

R

Q

N

D

G

L

H

A

A

R

A

A

R

H

E

R

F

A

A

I

A

H

R

R

V

L

N

E

V

A

G

G

M

I

V

C

G

W

I

I

N

N

V

T

-

W

-

G

-

E

-

S

P

P

I

A

P

E

V

M

P

P

V

V

A

-

Y

-

H

-

T

-

F

-

G

G

W

Y

K

K

R

Q

S

S

A

G

F

V

G

G

D

R

T
x
E

N

N

Q

-

H

-

G

G

M

L

E

T

G

T

I

L

K

A

F

H

F

Y

T

T

K

R

V

I

K

K

T

S

V

V

GAWN 150:153 (≠ TPFN 151:154) binding
K162 (≠ M163) active site, Charge relay system
KPSE 176:179 (= KPSE 177:180) binding
G209 (vs. gap) binding
GTST 230:233 (≠ SSDI 230:233) binding
E252 (≠ M251) active site, Proton acceptor
C286 (= C285) binding covalent; modified: Cysteine sulfenic acid (-SOH)
E387 (= E387) binding
E464 (≠ T465) active site, Charge relay system

4cazA Crystal structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa in complex with nadh
31% identity, 94% coverage: 11:482/503 of query aligns to 9:478/489 of 4cazA

query
sites
4cazA

F

Y

I

I

G

G

N

G

A

R

A

Y

T

V

A

E

G

A

T

S

G

S

T

G

R

A

R

T

S

F

D

E

V

T

F

I

D

N

P

P

N

A

T

N

G

G

A

E

V

V

Q

L

A

A

Q

K

V

V

T

Q

L

R

G

A

A

S

Q

R

A

E

D

D

L

V

D

E

A

R

A

A

M

V

A

Q

N

S

A

A

V

V

R

E

A

G

Q

Q

I

K

S

V

W

W

A

A

T

M

N

T

P

A

Q

M

R

Q

R

R

A

S

R

R

V

I

M

L

F

R

N

R

F

A

K

V

A

D

L

I

I

L

E

R

K

E

N

R

M

N

E

D

E

E

L

L

A

A

H

A

L

L

L

E

S

T

S

L

E

D

H

T

G

G

K

K

V

P

I

L

A

A

D

E

A

T

K

R

G

S

-

V

D

D

I

I

Q

V

R

T

G

G

L

A

E

D

V

V

I

L

E

E

F

Y

V

Y

C

A

G

G

I

L

P

V

H

P

V

A

L

I

K

E

G

G

E

E

Y

Q

T

I

Q

P

G

L

A

R

G

E

P

T

G

S

I

F

D

-

V

V

Y

Y

S

T

M

R

R

R

Q

E

P

P

L

L

G

G

V

V

A

A

G

G

I
|
I

T
x
G

P
x
A

F
x
W

N
|
N

F

Y

P

P

A

V

M

Q

I

I

P

A

M

L

W

W

M

K

F

S

G

A

V

P

A

A

I

L

A

A

C

A

G

G

N

N

A

A

F

M

I

I

L

F

K
|
K

P
|
P

S
|
S

E
|
E

R

V

D

T

P

P

S

L

V

T

P

A

V

L

R

K

L

L

A

A

E

E

L

I

M

Y

L

T

E

E

A

A

G

G

L

V

P

P

E

D

G

G

V

V

L

F

Q

N

V

V

V

L

Q

T

G

G

D

S

-
x
G

K

R

E

E

M

V

V
x
G

D
x
Q

A

W

I

L

L

T

D

E

H

H

P

P

E

L

I

I

K

E

A

K

V

I

S

S

F
|
F

V
x
T

G
|
G

S
x
G

S

T

D

S

I
x
T

A

G

H

K

Y

K

V
|
V

Y

M

R

A

R

S

G

A

V

S

A

S

G

S

-

L

K

K

R

E

V

V

Q

T

A

M

M
x
E

G
x
L

G
|
G

A

G

K

K

N

S

H

P

G

L

I

I

V

I

M

F

P

P

D

D

A

A

D

D

L

L

D

D

Q

R

V

A

N

D

D

I

L

A

A

V

G

M

A

A

A

N

F

F

G

F

S

S

A

S

G

G

E

Q

R

V

C
|
C

M

T

A

N

L

G

P

T

V

R

V

V

V

F

P

I

V

H

G

R

D

S

K

Q

T

Q

A

A

D

R

A

-

L

F

R

E

A

A

K

K

L

V

L

L

P

E

A

R

I

V

E

Q

A

R

L

I

R

R

V

L

G

G

V

D

S

P

T

Q

D

D

A

E

G

N

A

T

H

N

Y

F

G

G

P

P

V

L

V

V

T

S

A

F

A

P

H

H

K

M

A

E

K

S

I

V

E

L

S

G

Y

Y

I

I

Q

E

M

S

G

G

V

K

D

A

E

Q

G

K

A

A

E

R

L

L

V

L

V

C

D

G

G

G

R

E

G

R

F

V

T

T

L

D

Q

G

G

A

H

F

E

G

Q

K

G

G

F

A

F

Y

V

V

G

A

P

P

T

T

L

V

F

F

D

T

R

D

V

C

T

R

P

D

Q

D

M

M

Q

T

S

I

Y

V

Q

R

E

E

E
|
E

I

I

F
|
F

G

G

P

P

V

V

L

M

Q

S

I

I

V

L

R

V

A

Y

P

D

D

D

F

E

E

D

T

E

A

A

L

I

R

R

L

R

P

A

S

N

E

D

H

T

Q

E

Y

Y

G

G

N

L

G

A

V

A

A

G

I

V

F

V

T

T

R

Q

N

D

G

L

H

A

A

R

A

A

R

H

E

R

F

A

A

I

A

H

R

R

V

L

N

E

V

A

G

G

M

I

V

C

G

W

I

I

N

N

V

T

-

W

-

G

-

E

-

S

P

P

I

A

P

E

V

M

P

P

V

V

A

-

Y

-

H

-

T

-

F

-

G

G

W

Y

K

K

R

Q

S

S

A

G

F

V

G

G

D

R

T
x
E

N

N

Q

-

H

-

G

G

M

L

E

T

G

T

I

L

K

A

F

H

F

Y

T

T

K

R

V

I

K

K

T

S

V

V

active site: N152 (= N154), K175 (= K177), E251 (≠ M251), C285 (= C285), E386 (= E387), E463 (≠ T465)
binding [[(2R,3S,4R,5R)-5-[(3R)-3-aminocarbonyl-3,4-dihydro-2H-pyridin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanidyl-phosphoryl] [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl phosphate: I148 (= I150), G149 (≠ T151), A150 (≠ P152), W151 (≠ F153), N152 (= N154), K175 (= K177), P176 (= P178), S177 (= S179), E178 (= E180), G208 (vs. gap), G212 (≠ V213), Q213 (≠ D214), F226 (= F227), T227 (≠ V228), G228 (= G229), G229 (≠ S230), T232 (≠ I233), V236 (= V237), E251 (≠ M251), L252 (≠ G252), G253 (= G253), C285 (= C285), E386 (= E387), F388 (= F389)

2woxA Betaine aldehyde dehydrogenase from pseudomonas aeruginosa with NAD(p) h-catalytic thiol adduct. (see paper)
31% identity, 94% coverage: 11:482/503 of query aligns to 9:478/489 of 2woxA

query
sites
2woxA

F

Y

I

I

G

G

N

G

A

R

A

Y

T

V

A

E

G

A

T

S

G

S

T

G

R

A

R

T

S

F

D

E

V

T

F

I

D

N

P

P

N

A

T

N

G

G

A

E

V

V

Q

L

A

A

Q

K

V

V

T

Q

L

R

G

A

A

S

Q

R

A

E

D

D

L

V

D

E

A

R

A

A

M

V

A

Q

N

S

A

A

V

V

R

E

A

G

Q

Q

I

K

S

V

W

W

A

A

T

M

N

T

P

A

Q

M

R

Q

R

R

A

S

R

R

V

I

M

L

F

R

N

R

F

A

K

V

A

D

L

I

I

L

E

R

K

E

N

R

M

N

E

D

E

E

L

L

A

A

H

A

L

L

L

E

S

T

S

L

E

D

H

T

G

G

K

K

V

P

I

L

A

A

D

E

A

T

K

R

G

S

-

V

D

D

I

I

Q

V

R

T

G

G

L

A

E

D

V

V

I

L

E

E

F

Y

V

Y

C

A

G

G

I

L

P

V

H

P

V

A

L

I

K

E

G

G

E

E

Y

Q

T

I

Q

P

G

L

A

R

G

E

P

T

G

S

I

F

D

-

V

V

Y

Y

S

T

M

R

R

R

Q

E

P

P

L

L

G

G

V

V

A

A

G

G

I
|
I

T
x
G

P

A

F
x
W

N
|
N

F

Y

P

P

A

V

M

Q

I

I

P

A

M

L

W

W

M

K

F

S

G

A

V

P

A

A

I

L

A

A

C

A

G

G

N

N

A

A

F

M

I

I

L

F

K
|
K

P
|
P

S
|
S

E
|
E

R

V

D

T

P

P

S

L

V

T

P

A

V

L

R

K

L

L

A

A

E

E

L

I

M

Y

L

T

E

E

A

A

G

G

L

V

P

P

E

D

G

G

V

V

L

F

Q

N

V

V

V

L

Q

T

G

G

D
x
S

-
x
G

K
x
R

E

E

M

V

V
x
G

D
x
Q

A

W

I

L

L

T

D

E

H

H

P

P

E

L

I

I

K

E

A

K

V

I

S

S

F
|
F

V
x
T

G
|
G

S
x
G

S

T

D

S

I
x
T

A

G

H

K

Y

K

V
|
V

Y

M

R

A

R

S

G

A

V

S

A

S

G

S

-

L

K

K

R

E

V

V

Q

T

A

M

M
x
E

G
x
L

G
|
G

A

G

K

K

N

S

H

P

G

L

I

I

V

I

M

F

P

P

D

D

A

A

D

D

L

L

D

D

Q

R

V

A

N

D

D

I

L

A

A

V

G

M

A

A

A

N

F

F

G

F

S

S

A

S

G

G

E

Q

R

V

C
|
C

M

T

A

N

L

G

P

T

V

R

V

V

V

F

P

I

V

H

G

R

D

S

K

Q

T

Q

A

A

D

R

A

-

L

F

R

E

A

A

K

K

L

V

L

L

P

E

A

R

I

V

E

Q

A

R

L

I

R

R

V

L

G

G

V

D

S

P

T

Q

D

D

A

E

G

N

A

T

H

N

Y

F

G

G

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P

V

L

V

V

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A

F

A

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H

H

K

M

A

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K

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V

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G

G

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A

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V

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A

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P

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T

L

V

F

F

D

T

R

D

V

C

T

R

P

D

Q

D

M

M

Q

T

S

I

Y

V

Q

R

E

E

E
|
E

I

I

F
|
F

G

G

P

P

V

V

L

M

Q

S

I

I

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L

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D

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R

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Y

Y

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G

N

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V

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N

D

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L

H

A

A

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A

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A

A

I

A

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R

R

V

L

N

E

V

A

G

G

M

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C

G

W

I

I

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N

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-

W

-

G

-

E

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P

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A

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P

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V

V

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Y

-

H

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F

-

G

G

W

Y

K

K

R

Q

S

S

A

G

F

V

G

G

D

R

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x
E

N

N

Q

-

H

-

G

G

M

L

E

T

G

T

I

L

K

A

F

H

F

Y

T

T

K

R

V

I

K

K

T

S

V

V

active site: N152 (= N154), K175 (= K177), E251 (≠ M251), C285 (= C285), E386 (= E387), E463 (≠ T465)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I148 (= I150), G149 (≠ T151), W151 (≠ F153), N152 (= N154), K175 (= K177), P176 (= P178), S177 (= S179), E178 (= E180), S207 (≠ D209), G208 (vs. gap), R209 (≠ K210), G212 (≠ V213), Q213 (≠ D214), F226 (= F227), T227 (≠ V228), G228 (= G229), G229 (≠ S230), T232 (≠ I233), V236 (= V237), E251 (≠ M251), L252 (≠ G252), G253 (= G253), C285 (= C285), E386 (= E387), F388 (= F389)

2wmeA Crystallographic structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa (see paper)
31% identity, 94% coverage: 11:482/503 of query aligns to 9:478/489 of 2wmeA

query
sites
2wmeA

F

Y

I

I

G

G

N

G

A

R

A

Y

T

V

A

E

G

A

T

S

G

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D

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L

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G

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V

A

A

G

G

I
|
I

T
x
G

P

A

F
x
W

N
|
N

F

Y

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W

W

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G

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N

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|
K

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|
S

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|
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|
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G

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|
F

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x
T

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|
G

S
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G

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x
T

A

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|
V

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|
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|
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N

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G

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L

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A