SitesBLAST
Comparing Ga0059261_3686 Ga0059261_3686 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5y8lB Mycobacterium tuberculosis 3-hydroxyisobutyrate dehydrogenase (mthibadh) + NAD +(s)-3-hydroxyisobutyrate (s-hiba) (see paper)
47% identity, 98% coverage: 1:290/295 of query aligns to 1:288/290 of 5y8lB
- binding (2~{S})-2-methylpentanedioic acid: T129 (= T130), E149 (≠ A150), A152 (≠ G153), G153 (≠ K154), G153 (≠ K154), K154 (≠ A155)
- binding (2S)-2-methyl-3-oxidanyl-propanoic acid: S119 (= S120), G120 (= G121), W211 (= W212), F236 (= F237)
- binding nicotinamide-adenine-dinucleotide: G8 (= G8), G10 (= G10), N11 (= N11), M12 (= M12), F30 (= F30), D31 (= D31), P32 (≠ L32), M64 (= M64), L65 (= L65), T93 (= T94), G121 (= G122), K168 (= K169), L240 (= L241), K243 (= K244)
5y8kA Mycobacterium tuberculosis 3-hydroxyisobutyrate dehydrogenase (mthibadh) + l-serine (see paper)
47% identity, 98% coverage: 1:290/295 of query aligns to 1:288/290 of 5y8kA
P31937 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Homo sapiens (Human) (see paper)
44% identity, 98% coverage: 4:292/295 of query aligns to 42:332/336 of P31937
- LP 103:104 (= LP 65:66) binding
- N108 (≠ H70) binding
- T134 (= T94) binding
- K284 (= K244) binding
Sites not aligning to the query:
- 1:36 modified: transit peptide, Mitochondrion
- 40:68 binding
2i9pB Crystal structure of human hydroxyisobutyrate dehydrogenase complexed with NAD+
44% identity, 97% coverage: 4:290/295 of query aligns to 3:291/296 of 2i9pB
- binding nicotinamide-adenine-dinucleotide: G9 (= G10), N10 (= N11), M11 (= M12), Y29 (≠ F30), D30 (= D31), V31 (≠ L32), M63 (= M64), L64 (= L65), P65 (= P66), T95 (= T94), V120 (= V119), G122 (= G121), F238 (= F237), K245 (= K244)
5y8hA Mycobacterium tuberculosis 3-hydroxyisobutyrate dehydrogenase (mthibadh) + NAD+ (see paper)
47% identity, 97% coverage: 4:290/295 of query aligns to 3:287/291 of 5y8hA
- binding (2~{S})-2-methylpentanedioic acid: R144 (≠ E146), E148 (≠ A150), A151 (≠ G153), K153 (≠ A155)
- binding nicotinamide-adenine-dinucleotide: G7 (= G8), G9 (= G10), N10 (= N11), M11 (= M12), F29 (= F30), D30 (= D31), P31 (≠ L32), M63 (= M64), L64 (= L65), G120 (= G122), L239 (= L241), K242 (= K244)
5y8iA Mycobacterium tuberculosis 3-hydroxyisobutyrate dehydrogenase (mthibadh) + (s)-3-hydroxyisobutyrate (s-hiba) (see paper)
47% identity, 97% coverage: 4:290/295 of query aligns to 3:287/292 of 5y8iA
P29266 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Rattus norvegicus (Rat) (see paper)
43% identity, 98% coverage: 4:292/295 of query aligns to 41:331/335 of P29266
- D68 (= D31) mutation to R: Decrease of activity with NAD, increase of activity with NADP.
- K208 (= K169) mutation K->A,H,N,R: Complete loss of activity.
- N212 (= N173) mutation to Q: Decrease in activity.
Q9I5I6 NAD-dependent L-serine dehydrogenase; L-serine 3-dehydrogenase (NAD(+)); EC 1.1.1.387 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
47% identity, 98% coverage: 1:290/295 of query aligns to 1:292/298 of Q9I5I6
- 2:31 (vs. 2:31, 53% identical) binding
- P66 (= P66) binding
- T96 (= T94) binding ; mutation to A: Almost abolished activity.
- S122 (= S120) mutation to A: Strongly reduced activity.
- K171 (= K169) active site
- N175 (= N173) mutation to A: Strongly reduced activity.
- W214 (= W212) mutation to A: Almost abolished activity.
- Y219 (= Y217) mutation to A: Strongly reduced activity.
- K246 (= K244) binding ; mutation to A: Almost abolished activity.
- D247 (= D245) mutation to A: Almost abolished activity.
3q3cA Crystal structure of a serine dehydrogenase from pseudomonas aeruginosa pao1 in complex with NAD (see paper)
47% identity, 98% coverage: 3:290/295 of query aligns to 2:290/294 of 3q3cA
- binding nicotinamide-adenine-dinucleotide: G9 (= G10), H10 (≠ N11), M11 (= M12), F29 (= F30), D30 (= D31), L31 (= L32), M63 (= M64), L64 (= L65), P65 (= P66), T94 (= T94), V119 (= V119), G121 (= G121), F237 (= F237), K244 (= K244)
3obbA Crystal structure of a possible 3-hydroxyisobutyrate dehydrogenase from pseudomonas aeruginosa pao1 (see paper)
47% identity, 98% coverage: 1:290/295 of query aligns to 1:291/295 of 3obbA
P0A9V8 3-sulfolactaldehyde reductase; SLA reductase; 4-hydroxybutyrate dehydrogenase; Gamma-hydroxybutyrate dehydrogenase; GHBDH; Succinic semialdehyde reductase; SSA reductase; EC 1.1.1.373; EC 1.1.1.61 from Escherichia coli (strain K12)
37% identity, 98% coverage: 1:288/295 of query aligns to 1:284/298 of P0A9V8
- QM 11:12 (≠ NM 11:12) binding
- D31 (= D31) binding
- L65 (= L65) binding
- T96 (= T94) binding
- G122 (≠ S120) mutation to S: 25-fold decrease in catalytic efficiency with SLA as substrate. 5-fold decrease in catalytic efficiency with NADH as substrate.
- R123 (≠ G121) binding ; mutation to G: 130-fold decrease in catalytic efficiency with SLA as substrate. 3-fold decrease in catalytic efficiency with NADH as substrate.
- T124 (≠ G122) mutation to G: 230-fold decrease in catalytic efficiency with SLA as substrate. 12-fold decrease in catalytic efficiency with NADH as substrate.
- NNYMS 174:178 (≠ NNMLL 172:176) binding
- K240 (= K244) binding
5je8B The crystal structure of bacillus cereus 3-hydroxyisobutyrate dehydrogenase in complex with NAD (see paper)
31% identity, 99% coverage: 1:292/295 of query aligns to 3:291/294 of 5je8B
6smzC Crystal structure of sla reductase yihu from e. Coli in complex with nadh
37% identity, 98% coverage: 2:290/295 of query aligns to 1:285/295 of 6smzC
- binding nicotinamide-adenine-dinucleotide: G9 (= G10), Q10 (≠ N11), M11 (= M12), F29 (= F30), D30 (= D31), V31 (≠ L32), M63 (= M64), L64 (= L65), V73 (= V74), S94 (= S93), T95 (= T94), R122 (≠ G121)
6smyA Crystal structure of sla reductase yihu from e. Coli with nadh and product dhps
37% identity, 98% coverage: 2:290/295 of query aligns to 1:285/294 of 6smyA
3ws7A The 1.18 a resolution structure of l-serine 3-dehydrogenase complexed with NADP+ and sulfate ion from the hyperthermophilic archaeon pyrobaculum calidifontis (see paper)
36% identity, 95% coverage: 3:281/295 of query aligns to 15:287/293 of 3ws7A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G8), L21 (= L9), G22 (= G10), I23 (≠ N11), M24 (= M12), N43 (≠ D31), R44 (≠ L32), T45 (≠ S33), K48 (≠ A36), M76 (= M64), V77 (≠ L65), S78 (≠ P66), D82 (≠ H70), Q85 (= Q73), V133 (= V119), F241 (= F237), K242 (≠ A238), H245 (≠ L241), K248 (= K244)
- binding sulfate ion: T134 (≠ S120), G135 (= G121), K183 (= K169)
3w6zA Crystal structure of NADP bound l-serine 3-dehydrogenase (k170m) from hyperthermophilic archaeon pyrobaculum calidifontis (see paper)
35% identity, 95% coverage: 3:281/295 of query aligns to 15:290/296 of 3w6zA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G8), L21 (= L9), G22 (= G10), I23 (≠ N11), M24 (= M12), N43 (≠ D31), R44 (≠ L32), T45 (≠ S33), K48 (≠ A36), V77 (≠ L65), S78 (≠ P66), D82 (≠ H70), Q85 (= Q73), V133 (= V119), F244 (= F237), K245 (≠ A238), H248 (≠ L241), K251 (= K244)
2cvzC Structure of hydroxyisobutyrate dehydrogenase from thermus thermophilus hb8 (see paper)
34% identity, 92% coverage: 1:270/295 of query aligns to 1:260/289 of 2cvzC
- active site: S117 (= S120), K165 (= K169), N168 (= N172), N169 (= N173)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), L9 (= L9), G10 (= G10), A11 (≠ N11), M12 (= M12), N30 (vs. gap), R31 (= R28), T32 (≠ A29), C62 (≠ M64), L63 (= L65), P64 (= P66), E68 (≠ H70), E71 (≠ Q73), S91 (≠ T94), V116 (= V119), F227 (= F237), K234 (= K244)
1wp4A Structure of tt368 protein from thermus thermophilus hb8 (see paper)
34% identity, 91% coverage: 3:270/295 of query aligns to 2:259/288 of 1wp4A
- active site: S116 (= S120), K164 (= K169), N167 (= N172), N168 (= N173)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G8), L8 (= L9), G9 (= G10), A10 (≠ N11), M11 (= M12), N29 (vs. gap), R30 (= R28), T31 (≠ A29), K34 (≠ D35), C61 (≠ M64), L62 (= L65), P63 (= P66), E67 (≠ H70), S90 (≠ T94), V115 (= V119), T225 (≠ G236), F226 (= F237), K233 (= K244)
- binding sulfate ion: S116 (= S120), G117 (= G121), G118 (= G122), K164 (= K169)
3pefA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter metallireducens in complex with NADP+ (see paper)
32% identity, 97% coverage: 3:288/295 of query aligns to 3:283/287 of 3pefA
- binding glycerol: D67 (≠ A67), G123 (= G121), K171 (= K169), N175 (= N173), M178 (≠ L176), L203 (≠ Y201), G207 (≠ S207), N213 (≠ S213), A217 (≠ G222), F232 (= F237), H236 (≠ L241), K239 (= K244), R242 (≠ K247), R269 (≠ A274)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G10), I11 (≠ N11), M12 (= M12), N31 (≠ D31), R32 (≠ L32), S33 (= S33), K36 (≠ A36), M64 (= M64), L65 (= L65), A66 (≠ P66), A70 (≠ H70), E73 (≠ Q73), T96 (= T94), V121 (= V119), G123 (= G121), S124 (≠ G122), A231 (≠ G236), F232 (= F237), H236 (≠ L241), K239 (= K244)
3pduA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter sulfurreducens in complex with NADP+ (see paper)
31% identity, 98% coverage: 1:290/295 of query aligns to 1:285/287 of 3pduA
- binding glycerol: R242 (≠ K247), E246 (= E251), E246 (= E251), R250 (≠ S255)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), G10 (= G10), I11 (≠ N11), M12 (= M12), N31 (≠ D31), R32 (≠ L32), N33 (≠ S33), M64 (= M64), L65 (= L65), A66 (vs. gap), A70 (≠ G68), T96 (= T94), V121 (= V119), G123 (= G121), T124 (≠ G122), K171 (= K169), S231 (≠ G236), F232 (= F237), P233 (≠ A238), H236 (≠ L241), K239 (= K244)
Query Sequence
>Ga0059261_3686 Ga0059261_3686 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31)
MARVGFIGLGNMGGGMAANLAKAGHDVRAFDLSADALERAKAAGCLPVESAAAAAEGAEA
VITMLPAGKHVEQVYEDSVFGAADTGAILIDCSTIDVATARRVADAAQAKGLAAVDAPVS
GGIAAAAGGTLTFMVGGDAAAFERAEVFLAAMGKAVIHAGGNGAGQASKICNNMLLGATM
VATCEALLLAQKLGLDPQTFYDIASVSSGACWSLNTYAPLPGMGPQTPADNGYQGGFAAG
LMLKDLKLAMEAAESTHSETPMGARAAELYTKFADAGNAGVDFSGIIKMLGDGKV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory