SitesBLAST

Q6PIU2 Neutral cholesterol ester hydrolase 1; NCEH; Acetylalkylglycerol acetylhydrolase; 2-acetyl MAGE hydrolase; Arylacetamide deacetylase-like 1; EC 3.1.1.-; EC 3.1.1.71 from Homo sapiens (Human) (see 2 papers)
35% identity, 30% coverage: 59:144/286 of query aligns to 110:198/408 of Q6PIU2

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Q6PIU2

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Sites not aligning to the query:

287 modified: carbohydrate, N-linked (GlcNAc...) asparagine
343 L → M: in dbSNP:rs17857335

P24484 Lipase 2; Triacylglycerol lipase; EC 3.1.1.3 from Moraxella sp. (strain TA144) (see paper)
39% identity, 31% coverage: 58:147/286 of query aligns to 161:249/433 of P24484

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P24484

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H165 (= H62) mutation to Q: Loss of lipase and esterase activity.
S239 (= S137) mutation to A: Loss of lipase and esterase activity.

B5BLW5 Arylesterase; A-esterase; Paraoxonase; EC 3.1.1.2; EC 3.1.8.1 from Saccharolobus solfataricus (Sulfolobus solfataricus) (see paper)
27% identity, 66% coverage: 57:245/286 of query aligns to 77:269/306 of B5BLW5

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B5BLW5

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C105 (≠ G85) mutation to S: 3-fold loss of activity compared to wild-type.
C107 (≠ G87) mutation to S: 4-fold loss of activity compared to wild-type.
C129 (≠ V110) mutation to S: 28-fold loss of activity compared to wild-type.
S156 (= S137) mutation to A: Nearly loss of activity.
D251 (≠ E227) mutation to N: Nearly loss of activity.

Sites not aligning to the query:

281 H→N: 5-fold loss of activity compared to wild-type.

P22760 Arylacetamide deacetylase; EC 3.1.1.3 from Homo sapiens (Human) (see 6 papers)
27% identity, 56% coverage: 60:219/286 of query aligns to 109:278/399 of P22760

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P22760

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Sites not aligning to the query:

78 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→Q: Abolishes glycosylation at this site and causes moderate decrease in activity.
281 V → I: in alleles AADAC*2 and AADAC*3; mildly decreased enzyme activity; dbSNP:rs1803155
282 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→Q: Abolishes glycosylation at this site and causes substantial decrease in activity and reduced substrate affinity.
399 natural variant: L -> LQ (in allele AADAC*3; decreased enzyme activity)

4v2iA Biochemical characterization and structural analysis of a new cold- active and salt tolerant esterase from the marine bacterium thalassospira sp (see paper)
26% identity, 70% coverage: 56:254/286 of query aligns to 77:281/315 of 4v2iA

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4v2iA

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active site: A85 (≠ G64), G86 (= G65), S157 (= S137), V187 (vs. gap), F192 (≠ L169), D254 (≠ E227)
binding magnesium ion: Y185 (vs. gap), T250 (≠ H223), D254 (≠ E227), R257 (= R230)

Sites not aligning to the query:

active site: 284

Q7M370 Arylacetamide deacetylase; 50 kDa microsomal esterase/N-deacetylase; EC 3.1.1.3 from Oryctolagus cuniculus (Rabbit) (see 3 papers)
26% identity, 56% coverage: 60:219/286 of query aligns to 108:277/398 of Q7M370

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C115 (≠ K67) modified: Disulfide link with 339
S188 (= S137) active site

Sites not aligning to the query:

3 K→I: No effect on membrane topology. Inverted topology; when associated with N-24; Q-27 and Q-28, or with N-24; K-27 and K-28.
24 D→K: Inverted topology; when associated with I-3; Q-27 and Q-28, or with I-3; K-27 and K-28.
27:28 mutation EE->KK,QQ: No effect on topology; when associated with K-24. Inverted topology; when associated with I-3 and K-24.
339 modified: Disulfide link with 115

4ob6A Complex structure of esterase rppe s159a/w187h and substrate (s)-ac- cpa (see paper)
25% identity, 76% coverage: 42:257/286 of query aligns to 66:285/319 of 4ob6A

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L

I

V

L

Q

H

T

V

A

E

E

R

F

E
x
D

D

V

G

L

R

R

R

D

Q

E

S

G

E

E

A

A

L

Y

A

A

A

R

A

K

L

L

R

N

K

A

A

A

G

G

T

V

Q

T

A

-

E

-

V

V

R

T

A

S

L

V

R

R

G

Y

R

N

G

G

L

M

active site: G88 (= G64), G89 (= G65), A160 (≠ S137), V190 (vs. gap), F195 (vs. gap), D257 (≠ E227)
binding (2S)-(acetyloxy)(2-chlorophenyl)ethanoic acid: G88 (= G64), G89 (= G65), A160 (≠ S137), V161 (≠ A138), F208 (vs. gap), F208 (vs. gap), L209 (vs. gap)

Sites not aligning to the query:

active site: 287
binding (2S)-(acetyloxy)(2-chlorophenyl)ethanoic acid: 13, 37, 37, 40, 287, 288, 291, 292, 314, 315

6xycA Truncated form of carbohydrate esterase from gut microbiota (see paper)
28% identity, 40% coverage: 32:145/286 of query aligns to 14:129/285 of 6xycA

query
sites
6xycA

E

N

I

V

A

N

F

Y

G

A

A

S

D

D

P

S

L

L

Q

E

-

S

-

H

R

N

L

L

D

D

F

I

Y

Y

P

L

A

P

A

K

N

E

A

Q

S

K

G

T

A

A

A

Y

P

K

L

A

F

I

V

I

F

I

V

I

H

Y

G

G

G

S

G
x
A

W

W

K

F

R

A

G

N

D

N

K

A

R

K

N

A

A

M

T

A

G

S

A

A

A

S

K

I

I

G

A

A

H

P

F

L

T

L

G

K

R

A

G

G

H

F

A

A

F

V

A

I

S

S

V

I

N

N

Y

H

R

R

L

S

V

S

P

M

D

E

A

A

R

I

V

W

E

P

D

A

Q

Q

A

I

Q

Q

D

D

V

V

A

K

N

A

A

A

I

I

A

R

R

Y

L

V

I

R

R

S

D

N

A

A

R

A

S

K

L

Y

G

N

V

I

D

D

P

P

G

S

R

F

I

I

V

G

L

I

A

T

G

G

H

F

S
|
S

A
x
S

G

G

A

G

H

H

L

L

S

S

A

A

L

F

binding 4-(2-aminoethyl)benzenesulfonyl fluoride: A49 (≠ G65), S121 (= S137), S122 (≠ A138)

Sites not aligning to the query:

binding 4-(2-aminoethyl)benzenesulfonyl fluoride: 189, 190, 232, 262

6rkyA Structure of ester-hydrolase eh1ab1 from the metagenome of lake arreo complexed with a derivative of bipyridine phosphonate
29% identity, 45% coverage: 28:157/286 of query aligns to 48:178/315 of 6rkyA

query
sites
6rkyA

P

P

Q

E

P

V

R

A

E

E

I

V

A

A

F

N

G

G

-

G

-

F

A

A

D

G

P

P

L

A

Q

S

R

E

L

I

D

R

F

F

Y

R

-

R

-

Y

-

R

P

P

A

L

A

G

N

E

A

A

S

A

G

G

A

L

P

P

L

T

F

L

V

I

F

Y

V

Y

H

H

G
|
G

W

F

K

V

R

I

G

G

D

N

K

I

R

E

N

T

A

H

T

D

G

S

A

T

A

C

K

R

I

R

A

L

H

A

F

N

T

K

G

S

R

R

G

C

H

Q

A

V

F

I

A

-

S

S

V

I

N

D

Y

Y

R

R

L

L

V

A

P

P

D

E

A

H

R

P

V

F

E

P

D

A

Q

P

A

I

Q

D

D

D

V

G

A

I

N

A

A

A

I

F

A

R

R

H

L

I

I

R

R

D

D

N

A

A

R

E

S

S

L

F

G

G

V

A

D

D

P

A

G

A

R

R

I

L

V

A

L

V

A

G

G

G

H
x
D

S
|
S

A
|
A

G

G

A

G

H

A

L

M

S

A

A

A

L

V

V

V

A

C

T

-

D

-

P

-

Q

Q

Y

A

L

C

R

R

R

D

A

A

G

G

binding hexyl-[2-(3-oxidanylpyridin-2-yl)pyridin-3-yl]oxy-phosphinic acid: G88 (= G63), G89 (= G64), G90 (= G65), D160 (≠ H136), S161 (= S137), A162 (= A138)

Sites not aligning to the query:

binding hexyl-[2-(3-oxidanylpyridin-2-yl)pyridin-3-yl]oxy-phosphinic acid: 209, 218, 286

Query Sequence

>Ga0059261_3763 FitnessBrowser__Korea:Ga0059261_3763
MRFRSIVSPLILAALLAAPVAAQRPALPQPREIAFGADPLQRLDFYPAANASGAAPLFVF
VHGGGWKRGDKRNATGAAKIAHFTGRGHAFASVNYRLVPDARVEDQAQDVANAIARLIRD
ARSLGVDPGRIVLAGHSAGAHLSALVATDPQYLRRAGVGMERIKGVILLDGAAYDVPVQA
GEGPGIMRPTYTQAFGTDPARQRALSPTLHAAAPNAPRFLILHVEREDGRRQSEALAAAL
RKAGTQAEVRALRGRGLRGHMEINRALGDPDYPGTGIVDAWIDALP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory