SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ga0059261_3811 FitnessBrowser__Korea:Ga0059261_3811 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

A0R066 Branched-chain-amino-acid aminotransferase; BCAT; EC 2.6.1.42 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see 2 papers)
56% identity, 98% coverage: 6:361/362 of query aligns to 8:367/368 of A0R066

query
sites
A0R066

F

F

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T

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A

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D

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x
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R101 (= R99) binding pyridoxal 5'-phosphate
Y209 (= Y206) binding pyridoxal 5'-phosphate
K299 (≠ A293) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
T314 (= T308) binding pyridoxal 5'-phosphate

3dtgA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design
56% identity, 98% coverage: 6:361/362 of query aligns to 3:362/363 of 3dtgA

query
sites
3dtgA

F

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active site: K199 (= K201)
binding O-benzylhydroxylamine: F72 (= F75), Y171 (= Y173), Y204 (= Y206)
binding pyridoxal-5'-phosphate: R96 (= R99), R189 (= R191), K199 (= K201), Y204 (= Y206), E235 (= E237), G238 (= G240), L263 (= L262), I266 (= I265), T267 (= T266), T309 (= T308)

3dtfA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design (see paper)
56% identity, 98% coverage: 6:361/362 of query aligns to 3:362/363 of 3dtfA

query
sites
3dtfA

F

F

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A

A

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active site: K199 (= K201)
binding sulfate ion: R96 (= R99), I266 (= I265), T267 (= T266), T309 (= T308)

3ht5A Crystal structure of ilve a branched chain amino acid transaminase from mycobacterium tuberculosis (see paper)
57% identity, 91% coverage: 34:362/362 of query aligns to 3:335/335 of 3ht5A

query
sites
3ht5A

T

T

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R

R

W

Y

V

I

E

E

E
|
E

L

M

G

G

G
|
G

M
|
M

N
|
N

L

I

F

F

V

V

F

L

D

G

D

S

G

G

S

G

-

S

-

A

-

R

M

L

V

V

T

T

P

P

P

E

L

L

G

S

G

G

T

S

I

L

L
|
L

P

P

G
|
G

I
|
I

T
|
T

R

R

E

D

S

S

I

L

L

L

T

Q

L

L

A

A

R

I

E

D

Q

A

G

G

I

F

T

A

V

V

R

E

E

E

E

R

P

R

Y

I

A

D

I

I

D

D

Q

E

W

W

K

Q

A

K

D

K

A

A

G

A

S

A

G

G

K

E

L

I

V

T

E

E

T

V

F

F

A

A

C

C

G

G

T
|
T

A

A

V

V

V

I

T

T

P

P

V

V

G

A

K

R

V

V

K

R

S

H

R

G

D

A

G

S

E

E

F

F

T

R

I

I

G

A

S

D

G

G

G

Q

P

P

G

G

Q

E

V

V

T

T

E

M

A

A

L

L

K

R

A

D

R

T

L

L

T

T

A

G

I

I

Q

Q

R

R

G

G

Q

T

A

F

P

A

D

D

I

T

H

H

G

G

W

W

V

M

H

A

R

R

F

L

G

G

active site: K171 (= K201)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R68 (= R99), K171 (= K201), Y176 (= Y206), E207 (= E237), G210 (= G240), M211 (= M241), N212 (= N242), L235 (= L262), G237 (= G264), I238 (= I265), T239 (= T266), T281 (= T308)

5u3fA Structure of mycobacterium tuberculosis ilve, a branched-chain amino acid transaminase, in complex with d-cycloserine derivative (see paper)
55% identity, 91% coverage: 34:362/362 of query aligns to 2:326/326 of 5u3fA

query
sites
5u3fA

T

T

D

D

H

H

M

M

A

V

M

S

V

I

R

D

W

Y

S

A

D

E

T

G

K

R

G

G

W

W

H

H

D

N

A

A

K

R

V

V

V

I

A

P

R

Y

G

G

P

P

L

I

S

E

L

L

D

D

P

P

A

S

T

A

A

I

V

V

L

L

H

H

Y

Y

A

A

Q

Q

E

E

I

V

F

F

E

E

G
|
G

L

L

K

K

A

A

Y

Y

R

R

T

W

G

A

D

D

E

G

G

S

T

I

A

V

L

S

F

F

R

R

P

A

L

D

E

A

N

N

A

A

R

A

R
|
R

F

L

R

R

E

S

S

S

A

A

Q

R

R

R

M

L

A

A

M

I

P

P

E

E

L

L

P

P

D

D

D

A

L

V

F

F

L

I

G

E

S

S

I

L

E

R

A

Q

L

L

V

I

K

A

A

V

D

D

R

K

E

A

W

W

I

V

P

P

Q

G

I

A

E

G

G

G

G

E

-

E

S

A

L

L

Y
|
Y

L

L

R

R

P

P

F

F

M

I

F

F

A

A

S

T

E

E

V

P

F

G

L

L

G

G

V

V

K

R

P

P

A

A

S

T

E

Q

Y

Y

L

R

Y

Y

L

L

V

L

I

I

A

A

S

S

P

P

A

A

G

-

A

-

Y

-

F

-

K

-

G

-

A

A

P

P

A

-

V

V

T

S

I

V

W

W

V

V

S

S

D

T

H

E

Y

Y

T

V

R

R

A

A

A

C

P

P

G

G

T

T

G

G

A

A

K
|
K

C

F

G

G

N

N

Y
|
Y

A

A

S

A

S

S

L

L

V

L

A

A

Q

Q

A

A

E

E

A

A

I

A

R

E

E

N

G

G

C

C

D

D

Q

Q

V

V

F

W

L

L

D

D

A

A

V

V

E

E

R

R

W

Y

V

I

E

E

E
|
E

L

M

G

G

G
|
G

M
|
M

N
|
N

L

I

F

F

V

V

F

L

D

G

D

S

G

G

S

G

-

S

-

A

-

R

M

L

V

V

T

T

P

P

P

E

L

L

G

S

G

G

T

S

I

L

L
|
L

P

P

G
|
G

I
|
I

T
|
T

R

R

E

D

S

S

I

L

L

L

T

Q

L

L

A

A

R

I

E

D

Q

A

G

G

I

F

T

A

V

V

R

E

E

E

E

R

P

R

Y

I

A

D

I

I

D

D

Q

E

W

W

K

Q

A

K

D

K

A

A

G

A

S

A

G

G

K

E

L

I

V

T

E

E

T

V

F

F

A

A

C

C

G

G

T
|
T

A

A

V

V

V

I

T

T

P

P

V

V

G

A

K

R

V

V

K

R

S

H

R

G

D

A

G

S

E

E

F

F

T

R

I

I

G

A

S

D

G

G

G

Q

P

P

G

G

Q

E

V

V

T

T

E

M

A

A

L

L

K

R

A

D

R

T

L

L

T

T

A

G

I

I

Q

Q

R

R

G

G

Q

T

A

F

P

A

D

D

I

T

H

H

G

G

W

W

V

M

H

A

R

R

F

L

G

G

active site: K162 (= K201)
binding (5-hydroxy-6-methyl-4-{[(3-oxo-2,3-dihydro-1,2-oxazol-4-yl)amino]methyl}pyridin-3-yl)methyl dihydrogen phosphate: G45 (= G77), R67 (= R99), Y110 (= Y141), K162 (= K201), Y167 (= Y206), E198 (= E237), G201 (= G240), M202 (= M241), N203 (= N242), L226 (= L262), G228 (= G264), I229 (= I265), T230 (= T266), T272 (= T308)

7nyaA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor bay-069 (compound 36) (see paper)
41% identity, 90% coverage: 25:350/362 of query aligns to 23:354/364 of 7nyaA

query
sites
7nyaA

E

N

N

N

P

L

G

V

F
|
F

G

G

K

T

L

V

F

F

T

T

D

D

H

H

M

M

A

L

M

T

V

V

R

E

W

W

S

S

D

S

T

E

K

F

G

G

W

W

H

E

D

K

A

P

K

H

V

I

V

K

A

P

R

L

G

Q

P

N

L

L

S

S

L

L

D

H

P

P

A

G

T

S

A

S

V

A

L

L

H

H

Y
|
Y

A

A

Q

V

E

E

I

L

F
|
F

E

E

G

G

L

L

K

K

A

A

Y

F

R

R

T

G

G

V

D

D

E

N

G

K

T

I

A

R

L

L

F

F

R

Q

P

P

L

N

E

L

N

N

A

M

R

D

R
|
R

F

M

R

Y

E

R

S

S

A

A

Q

V

R

R

M

A

A

T

M

L

P

P

E

V

L

F

P

D

D

K

D

E

L

E

F

L

L

L

G

E

S

C

I

I

E

Q

A

Q

L

L

V

V

K

K

A

L

D

D

R

Q

E

E

W

W

I

V

P

P

Q

Y

I

S

E

T

G

S

G

A

S

S

L

L

Y
|
Y

L

I

R

R

P

P

F

T

M

F

F

I

A

G

S

T

E

E

V

P

F

S

L

L

G
|
G

V
|
V

K

K

P

K

A

P

S

T

E

K

Y

A

L

L

Y

L

L

F

V

V

I

L

A

L

S

S

P

P

A

V

G

G

A

P

Y
|
Y

F

F

K

S

G

S

G

G

A

T

-

F

P

N

A

P

V

V

T

S

I

L

W

W

V

A

S

N

D

P

H

K

Y

Y

T

V

R
|
R

A

A

A

W

P

K

G

G

T

T

G

G

A

D

A

C

K
|
K

C

M

G

G

N

N

Y
|
Y

A

G

S

S

L

L

V

F

A

A

Q
|
Q

A

C

E

E

A

A

I

V

R

D

E

N

G

G

C

C

D

Q

Q
|
Q

V

V

V

L

F

W

L

L

D

Y

A

G

V

-

E

E

R

D

R

H

W

Q

V

I

E

T

E
|
E

L

V

G

G

G
x
T

M
|
M

N

N

L

L

F

F

-

L

-

Y

F

W

V

I

F

N

D

E

D

D

G

G

-

E

S

E

M

L

V

A

T

T

P

P

L

L

G

D

G

G

T

I

I

I

L
|
L

P

P

G
|
G

I
x
V

T
|
T

R

R

E

R

S

C

I

I

L

L

T

D

L

L

A

A

R

H

E

Q

Q

W

G

G

-

E

I

F

T

K

V

V

R

S

E

E

E

R

P

Y

Y

L

A

T

I

M

D

D

Q

D

W

L

K

T

A

T

D

A

A

L

G

E

S

G

G

N

K

R

L

V

V

R

E

E

T

M

F

F

A

G

C

S

G
|
G

T
|
T

A
|
A

A

C

V

V

T

C

P

P

V

V

G

S

K

D

V

I

K

L

S

Y

R

K

D

G

G

E

E

T

F

I

T

H

I

I

G

P

S

T

-

M

-

E

G

N

G

G

P

P

G

K

Q

L

V

A

T

S

E

R

A

I

L

L

K

-

A

S

R

K

L

L

T

T

A

D

I

I

Q

Q

R

Y

G

G

Q

R

binding pyridoxal-5'-phosphate: R97 (= R99), R190 (= R191), K200 (= K201), Y205 (= Y206), E235 (= E237), T238 (≠ G240), M239 (= M241), L264 (= L262), G266 (= G264), V267 (≠ I265), T268 (= T266), G310 (= G307), T311 (= T308)
binding 3-[4-chloranyl-3-(2-methylphenoxy)naphthalen-1-yl]-6-(trifluoromethyl)-5~{H}-pyrimidine-2,4-dione: F27 (= F29), Y68 (= Y70), F73 (= F75), Y139 (= Y141), G152 (= G154), V153 (= V155), Y171 (= Y173), Q212 (= Q213), Q222 (= Q223), T238 (≠ G240), A312 (= A309)

7ny9A Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor compound 38 (see paper)
41% identity, 90% coverage: 25:350/362 of query aligns to 23:354/364 of 7ny9A

query
sites
7ny9A

E

N

N

N

P

L

G

V

F
|
F

G

G

K

T

L

V

F

F

T

T

D

D

H

H

M

M

A

L

M

T

V

V

R

E

W

W

S

S

D

S

T

E

K

F

G

G

W

W

H

E

D

K

A

P

K

H

V

I

V

K

A

P

R

L

G

Q

P

N

L

L

S

S

L

L

D

H

P

P

A

G

T

S

A

S

V

A

L

L

H

H

Y

Y

A

A

Q

V

E

E

I

L

F

F

E

E

G

G

L

L

K

K

A

A

Y

F

R

R

T

G

G

V

D

D

E

N

G

K

T

I

A

R

L

L

F

F

R

Q

P

P

L

N

E

L

N

N

A

M

R

D

R
|
R

F

M

R

Y

E

R

S

S

A

A

Q

V

R

R

M

A

A

T

M

L

P

P

E

V

L

F

P

D

D

K

D

E

L

E

F

L

L

L

G

E

S

C

I

I

E

Q

A

Q

L

L

V

V

K

K

A

L

D

D

R

Q

E

E

W

W

I

V

P

P

Q

Y

I

S

E

T

G

S

G

A

S

S

L

L

Y
|
Y

L

I

R

R

P

P

F

T

M

F

F

I

A

G

S

T

E

E

V

P

F

S

L

L

G
|
G

V
|
V

K

K

P

K

A

P

S

T

E

K

Y

A

L

L

Y

L

L

F

V

V

I

L

A

L

S

S

P

P

A

V

G

G

A

P

Y
|
Y

F

F

K

S

G

S

G

G

A

T

-

F

P

N

A

P

V

V

T

S

I

L

W

W

V

A

S

N

D

P

H

K

Y

Y

T

V

R
|
R

A

A

A

W

P

K

G

G

T

T

G

G

A

D

A

C

K
|
K

C

M

G

G

N

N

Y
|
Y

A

G

S

S

L

L

V

F

A

A

Q
|
Q

A

C

E

E

A

A

I

V

R

D

E

N

G

G

C

C

D
x
Q

Q
|
Q

V
|
V

V

L

F

W

L

L

D

Y

A

G

V

-

E

E

R

D

R

H

W

Q

V

I

E

T

E
|
E

L

V

G

G

G
x
T

M
|
M

N
|
N

L

L

F

F

-

L

-

Y

F

W

V

I

F

N

D

E

D

D

G

G

-

E

S

E

M

L

V

A

T

T

P

P

L

L

G

D

G

G

T

I

I

I

L
|
L

P

P

G
|
G

I
x
V

T
|
T

R

R

E

R

S

C

I

I

L

L

T

D

L

L

A

A

R

H

E

Q

Q

W

G

G

-

E

I

F

T

K

V

V

R

S

E

E

E

R

P

Y

Y

L

A

T

I

M

D

D

Q

D

W

L

K

T

A

T

D

A

A

L

G

E

S

G

G

N

K

R

L

V

V

R

E

E

T

M

F

F

A

G

C

S

G

G

T
|
T

A

A

A

C

V

V

T

C

P

P

V

V

G

S

K

D

V

I

K

L

S

Y

R

K

D

G

G

E

E

T

F

I

T

H

I

I

G

P

S

T

-

M

-

E

G

N

G

G

P

P

G

K

Q

L

V

A

T

S

E

R

A

I

L

L

K

-

A

S

R

K

L

L

T

T

A

D

I

I

Q

Q

R

Y

G

G

Q

R

binding pyridoxal-5'-phosphate: R97 (= R99), R190 (= R191), K200 (= K201), Y205 (= Y206), E235 (= E237), T238 (≠ G240), M239 (= M241), N240 (= N242), L264 (= L262), G266 (= G264), V267 (≠ I265), T268 (= T266), T311 (= T308)
binding 3-[2,6-bis(oxidanylidene)-4-(trifluoromethyl)-5~{H}-pyrimidin-1-yl]-~{N}-methyl-2-pyridin-2-yl-imidazo[1,2-a]pyridine-7-carboxamide: F27 (= F29), Y139 (= Y141), G152 (= G154), V153 (= V155), Y171 (= Y173), Q212 (= Q213), Q221 (≠ D222), Q222 (= Q223), V223 (= V224), T238 (≠ G240)

7ny2A Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor compound 35 (see paper)
41% identity, 90% coverage: 25:350/362 of query aligns to 23:354/364 of 7ny2A

query
sites
7ny2A

E

N

N

N

P

L

G

V

F
|
F

G

G

K

T

L

V

F

F

T

T

D

D

H

H

M

M

A

L

M

T

V

V

R

E

W

W

S

S

D

S

T

E

K

F

G

G

W

W

H

E

D

K

A

P

K

H

V

I

V

K

A

P

R

L

G

Q

P

N

L

L

S

S

L

L

D

H

P

P

A

G

T

S

A

S

V

A

L

L

H

H

Y

Y

A

A

Q

V

E

E

I

L

F

F

E

E

G

G

L

L

K

K

A

A

Y

F

R

R

T

G

G

V

D

D

E

N

G

K

T

I

A

R

L

L

F

F

R

Q

P

P

L

N

E

L

N

N

A

M

R

D

R
|
R

F

M

R

Y

E

R

S

S

A

A

Q

V

R

R

M

A

A

T

M

L

P

P

E

V

L

F

P

D

D

K

D

E

L

E

F

L

L

L

G

E

S

C

I

I

E

Q

A

Q

L

L

V

V

K

K

A

L

D

D

R

Q

E

E

W

W

I

V

P

P

Q

Y

I

S

E

T

G

S

G

A

S

S

L

L

Y
|
Y

L

I

R

R

P

P

F

T

M

F

F

I

A

G

S

T

E

E

V

P

F

S

L

L

G
|
G

V
|
V

K

K

P

K

A

P

S

T

E

K

Y

A

L

L

Y

L

L

F

V

V

I

L

A

L

S

S

P

P

A

V

G

G

A

P

Y
|
Y

F

F

K

S

G

S

G

G

A

T

-

F

P

N

A

P

V

V

T

S

I

L

W

W

V

A

S

N

D

P

H

K

Y

Y

T

V

R

R

A

A

A

W

P

K

G

G

T

T

G

G

A

D

A

C

K
|
K

C

M

G

G

N

N

Y
|
Y

A

G

S

S

L

L

V

F

A

A

Q
|
Q

A

C

E

E

A

A

I

V

R

D

E

N

G

G

C

C

D

Q

Q
|
Q

V

V

V

L

F

W

L

L

D

Y

A

G

V

-

E

E

R

D

R

H

W

Q

V

I

E

T

E
|
E

L

V

G

G

G
x
T

M
|
M

N

N

L

L

F

F

-

L

-

Y

F

W

V

I

F

N

D

E

D

D

G

G

-

E

S

E

M

L

V

A

T

T

P

P

L

L

G

D

G

G

T

I

I

I

L
|
L

P

P

G
|
G

I
x
V

T
|
T

R

R

E

R

S

C

I

I

L

L

T

D

L

L

A

A

R

H

E

Q

Q

W

G

G

-

E

I

F

T

K

V

V

R

S

E

E

E

R

P

Y

Y

L

A

T

I

M

D

D

Q

D

W

L

K

T

A

T

D

A

A

L

G

E

S

G

G

N

K

R

L

V

V

R

E

E

T

M

F

F

A

G

C

S

G

G

T
|
T

A
|
A

A

C

V

V

T

C

P

P

V

V

G

S

K

D

V

I

K

L

S

Y

R

K

D

G

G

E

E

T

F

I

T

H

I

I

G

P

S

T

-

M

-

E

G

N

G

G

P

P

G

K

Q

L

V

A

T

S

E

R

A

I

L

L

K

-

A

S

R

K

L

L

T

T

A

D

I

I

Q

Q

R

Y

G

G

Q

R

binding pyridoxal-5'-phosphate: R97 (= R99), K200 (= K201), Y205 (= Y206), E235 (= E237), T238 (≠ G240), M239 (= M241), L264 (= L262), G266 (= G264), V267 (≠ I265), T268 (= T266), T311 (= T308)
binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-2-(2-methylphenoxy)naphthalene-1-carbonitrile: F27 (= F29), Y139 (= Y141), G152 (= G154), V153 (= V155), Y171 (= Y173), Q212 (= Q213), Q222 (= Q223), A312 (= A309)

P54687 Branched-chain-amino-acid aminotransferase, cytosolic; BCAT(c); EC 2.6.1.42 from Homo sapiens (Human) (see 2 papers)
41% identity, 90% coverage: 25:350/362 of query aligns to 45:376/386 of P54687

query
sites
P54687

E

N

N

N

P

L

G

V

F

F

G

G

K

T

L

V

F

F

T

T

D

D

H

H

M

M

A

L

M

T

V

V

R

E

W

W

S

S

D

S

T

E

K

F

G

G

W

W

H

E

D

K

A

P

K

H

V

I

V

K

A

P

R

L

G

Q

P

N

L

L

S

S

L

L

D

H

P

P

A

G

T

S

A

S

V

A

L

L

H

H

Y

Y

A

A

Q

V

E

E

I

L

F

F

E

E

G

G

L

L

K

K

A

A

Y

F

R

R

T

G

G

V

D

D

E

N

G

K

T

I

A

R

L

L

F

F

R

Q

P

P

L

N

E

L

N

N

A

M

R

D

R

R

F

M

R

Y

E

R

S

S

A

A

Q

V

R

R

M

A

A

T

M

L

P

P

E

V

L

F

P

D

D

K

D

E

L

E

F

L

L

L

G

E

S

C

I

I

E

Q

A

Q

L

L

V

V

K

K

A

L

D

D

R

Q

E

E

W

W

I

V

P

P

Q

Y

I

S

E

T

G

S

G

A

S

S

L

L

Y

Y

L

I

R

R

P

P

F

T

M

F

F

I

A

G

S

T

E

E

V

P

F

S

L

L

G

G

V

V

K

K

P

K

A

P

S

T

E

K

Y

A

L

L

Y

L

L

F

V

V

I

L

A

L

S

S

P

P

A

V

G

G

A

P

Y

Y

F

F

K

S

G

S

G

G

A

T

-

F

P

N

A

P

V

V

T

S

I

L

W

W

V

A

S

N

D

P

H

K

Y

Y

T

V

R

R

A

A

A

W

P

K

G

G

T

T

G

G

A

D

A

C

K
|
K

C

M

G

G

N

N

Y

Y

A

G

S

S

L

L

V

F

A

A

Q

Q

A

C

E

E

A

A

I

V

R

D

E

N

G

G

C

C

D

Q

Q

Q

V

V

V

L

F

W

L

L

D

Y

A

G

V

-

E

E

R

D

R

H

W

Q

V

I

E

T

E

E

L

V

G

G

G

T

M

M

N

N

L

L

F

F

-

L

-

Y

F

W

V

I

F

N

D

E

D

D

G

G

-

E

S

E

M

L

V

A

T

T

P

P

L

L

G

D

G

G

T

I

I

I

L

L

P

P

G

G

I

V

T

T

R

R

E

R

S

C

I

I

L

L

T

D

L

L

A

A

R

H

E

Q

Q

W

G

G

-

E

I

F

T

K

V

V

R

S

E

E

E

R

P

Y

Y

L

A

T

I

M

D

D

Q

D

W

L

K

T

A

T

D

A

A

L

G

E

S

G

G

N

K

R

L

V

V

R

E

E

T

M

F

F

A
x
G

C

S

G

G

T

T

A

A

A

C

V

V

T

C

P

P

V

V

G

S

K

D

V

I

K

L

S

Y

R

K

D

G

G

E

E

T

F

I

T

H

I

I

G

P

S

T

-

M

-

E

G

N

G

G

P

P

G

K

Q

L

V

A

T

S

E

R

A

I

L

L

K

-

A

S

R

K

L

L

T

T

A

D

I

I

Q

Q

R

Y

G

G

Q

R

K222 (= K201) modified: N6-(pyridoxal phosphate)lysine
G330 (≠ A305) to S: in dbSNP:rs1057204

7nwmA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 12 (see paper)
41% identity, 90% coverage: 25:350/362 of query aligns to 24:355/365 of 7nwmA

query
sites
7nwmA

E

N

N

N

P

L

G

V

F
|
F

G

G

K

T

L

V

F

F

T

T

D

D

H

H

M

M

A

L

M

T

V

V

R

E

W

W

S

S

D

S

T

E

K

F

G

G

W

W

H

E

D

K

A

P

K

H

V

I

V

K

A

P

R

L

G

Q

P

N

L

L

S

S

L

L

D

H

P

P

A

G

T

S

A

S

V

A

L

L

H

H

Y
|
Y

A

A

Q

V

E

E

I

L

F
|
F

E

E

G

G

L

L

K

K

A

A

Y

F

R

R

T

G

G

V

D

D

E

N

G

K

T

I

A

R

L

L

F

F

R

Q

P

P

L

N

E

L

N

N

A

M

R

D

R
|
R

F

M

R

Y

E

R

S

S

A

A

Q

V

R

R

M

A

A

T

M

L

P

P

E

V

L

F

P

D

D

K

D

E

L

E

F

L

L

L

G

E

S

C

I

I

E

Q

A

Q

L

L

V

V

K

K

A

L

D

D

R

Q

E

E

W

W

I

V

P

P

Q

Y

I

S

E

T

G

S

G

A

S

S

L

L

Y
|
Y

L

I

R

R

P

P

F

T

M

F

F

I

A

G

S

T

E

E

V

P

F

S

L

L

G
|
G

V
|
V

K

K

P

K

A

P

S

T

E

K

Y

A

L

L

Y

L

L

F

V

V

I

L

A

L

S

S

P

P

A

V

G

G

A

P

Y
|
Y

F

F

K

S

G

S

G

G

A

T

-

F

P

N

A

P

V

V

T

S

I

L

W

W

V

A

S

N

D

P

H

K

Y

Y

T

V

R
|
R

A

A

A

W

P

K

G

G

T

T

G

G

A

D

A

C

K
|
K

C

M

G

G

N

N

Y
|
Y

A

G

S

S

L

L

V

F

A

A

Q
|
Q

A

C

E

E

A

A

I

V

R

D

E

N

G

G

C

C

D

Q

Q
|
Q

V

V

V

L

F

W

L

L

D

Y

A

G

V

-

E

E

R

D

R

H

W

Q

V

I

E

T

E
|
E

L

V

G

G

G
x
T

M
|
M

N

N

L

L

F

F

-

L

-

Y

F

W

V

I

F

N

D

E

D

D

G

G

-

E

S

E

M

L

V

A

T

T

P

P

L

L

G

D

G

G

T

I

I

I

L
|
L

P

P

G
|
G

I
x
V

T
|
T

R

R

E

R

S

C

I

I

L

L

T

D

L

L

A

A

R

H

E

Q

Q

W

G

G

-

E

I

F

T

K

V

V

R

S

E

E

E

R

P

Y

Y

L

A

T

I

M

D

D

Q

D

W

L

K

T

A

T

D

A

A

L

G

E

S

G

G

N

K

R

L

V

V

R

E

E

T

M

F

F

A

G

C

S

G
|
G

T
|
T

A
|
A

A

C

V

V

T

C

P

P

V

V

G

S

K

D

V

I

K

L

S

Y

R

K

D

G

G

E

E

T

F

I

T

H

I

I

G

P

S

T

-

M

-

E

G

N

G

G

P

P

G

K

Q

L

V

A

T

S

E

R

A

I

L

L

K

-

A

S

R

K

L

L

T

T

A

D

I

I

Q

Q

R

Y

G

G

Q

R

binding pyridoxal-5'-phosphate: R98 (= R99), R191 (= R191), K201 (= K201), Y206 (= Y206), E236 (= E237), T239 (≠ G240), M240 (= M241), L265 (= L262), G267 (= G264), V268 (≠ I265), T269 (= T266), G311 (= G307), T312 (= T308)
binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-5-methoxy-2-(2-methylphenoxy)benzenecarbonitrile: F28 (= F29), Y69 (= Y70), F74 (= F75), Y140 (= Y141), G153 (= G154), V154 (= V155), Y172 (= Y173), Q213 (= Q213), Q223 (= Q223), T239 (≠ G240), A313 (= A309)

7nweA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 10 (see paper)
41% identity, 90% coverage: 25:350/362 of query aligns to 24:355/365 of 7nweA

query
sites
7nweA

E

N

N

N

P

L

G

V

F
|
F

G

G

K

T

L

V

F

F

T

T

D

D

H

H

M

M

A

L

M

T

V

V

R

E

W

W

S

S

D

S

T

E

K

F

G

G

W

W

H

E

D

K

A

P

K

H

V

I

V

K

A

P

R

L

G

Q

P

N

L

L

S

S

L

L

D

H

P

P

A

G

T

S

A

S

V

A

L

L

H

H

Y
|
Y

A

A

Q

V

E

E

I

L

F
|
F

E

E

G

G

L

L

K

K

A

A

Y

F

R

R

T

G

G

V

D

D

E

N

G

K

T

I

A

R

L

L

F

F

R

Q

P

P

L

N

E

L

N

N

A

M

R

D

R
|
R

F

M

R

Y

E

R

S

S

A

A

Q

V

R

R

M

A

A

T

M

L

P

P

E

V

L

F

P

D

D

K

D

E

L

E

F

L

L

L

G

E

S

C

I

I

E

Q

A

Q

L

L

V

V

K

K

A

L

D

D

R

Q

E

E

W

W

I

V

P

P

Q

Y

I

S

E

T

G

S

G

A

S

S

L

L

Y
|
Y

L

I

R

R

P

P

F

T

M

F

F

I

A

G

S

T

E

E

V

P

F

S

L

L

G
|
G

V
|
V

K

K

P

K

A

P

S

T

E

K

Y

A

L

L

Y

L

L

F

V

V

I

L

A

L

S

S

P

P

A

V

G

G

A

P

Y
|
Y

F

F

K

S

G

S

G

G

A

T

-

F

P

N

A

P

V

V

T

S

I

L

W

W

V

A

S

N

D

P

H

K

Y

Y

T

V

R

R

A

A

A

W

P

K

G

G

T

T

G

G

A

D

A

C

K
|
K

C

M

G

G

N

N

Y
|
Y

A

G

S

S

L

L

V

F

A

A

Q
|
Q

A

C

E

E

A

A

I

V

R

D

E

N

G

G

C

C

D

Q

Q
|
Q

V

V

V

L

F

W

L

L

D

Y

A

G

V

-

E

E

R

D

R

H

W

Q

V

I

E

T

E
|
E

L

V

G

G

G
x
T

M
|
M

N

N

L

L

F

F

-

L

-

Y

F

W

V

I

F

N

D

E

D

D

G

G

-

E

S

E

M

L

V

A

T

T

P

P

L

L

G

D

G

G

T

I

I

I

L
|
L

P

P

G
|
G

I
x
V

T
|
T

R

R

E

R

S

C

I

I

L

L

T

D

L

L

A

A

R

H

E

Q

Q

W

G

G

-

E

I

F

T

K

V

V

R

S

E

E

E

R

P

Y

Y

L

A

T

I

M

D

D

Q

D

W

L

K

T

A

T

D

A

A

L

G

E

S

G

G

N

K

R

L

V

V

R

E

E

T

M

F

F

A

G

C

S

G
|
G

T
|
T

A
|
A

A

C

V

V

T

C

P

P

V

V

G

S

K

D

V

I

K

L

S

Y

R

K

D

G

G

E

E

T

F

I

T

H

I

I

G

P

S

T

-

M

-

E

G

N

G

G

P

P

G

K

Q

L

V

A

T

S

E

R

A

I

L

L

K

-

A

S

R

K

L

L

T

T

A

D

I

I

Q

Q

R

Y

G

G

Q

R

binding pyridoxal-5'-phosphate: R98 (= R99), K201 (= K201), Y206 (= Y206), E236 (= E237), T239 (≠ G240), M240 (= M241), L265 (= L262), G267 (= G264), V268 (≠ I265), T269 (= T266), G311 (= G307), T312 (= T308)
binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-5-methyl-2-(2-methylphenoxy)benzenecarbonitrile: F28 (= F29), Y69 (= Y70), F74 (= F75), Y140 (= Y141), G153 (= G154), V154 (= V155), Y172 (= Y173), Q213 (= Q213), Q223 (= Q223), T239 (≠ G240), A313 (= A309)

7nwcA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 2 (see paper)
41% identity, 90% coverage: 25:350/362 of query aligns to 24:355/365 of 7nwcA

query
sites
7nwcA

E

N

N

N

P

L

G

V

F
|
F

G

G

K

T

L

V

F

F

T

T

D

D

H

H

M

M

A

L

M

T

V

V

R

E

W

W

S

S

D

S

T

E

K

F

G

G

W

W

H

E

D

K

A

P

K

H

V

I

V

K

A

P

R

L

G

Q

P

N

L

L

S

S

L

L

D

H

P

P

A

G

T

S

A

S

V

A

L

L

H

H

Y

Y

A

A

Q

V

E

E

I

L

F

F

E

E

G

G

L

L

K

K

A

A

Y

F

R

R

T

G

G

V

D

D

E

N

G

K

T

I

A

R

L

L

F

F

R

Q

P

P

L

N

E

L

N

N

A

M

R

D

R
|
R

F

M

R

Y

E

R

S

S

A

A

Q

V

R

R

M

A

A

T

M

L

P

P

E

V

L

F

P

D

D

K

D

E

L

E

F

L

L

L

G

E

S

C

I

I

E

Q

A

Q

L

L

V

V

K

K

A

L

D

D

R

Q

E

E

W

W

I

V

P

P

Q

Y

I

S

E

T

G

S

G

A

S

S

L

L

Y

Y

L

I

R

R

P

P

F

T

M

F

F

I

A

G

S

T

E

E

V

P

F

S

L

L

G

G

V

V

K

K

P

K

A

P

S

T

E

K

Y

A

L

L

Y

L

L

F

V

V

I

L

A

L

S

S

P

P

A

V

G

G

A

P

Y
|
Y

F

F

K

S

G

S

G

G

A

T

-

F

P

N

A

P

V

V

T

S

I

L

W

W

V

A

S

N

D

P

H

K

Y

Y

T

V

R
|
R

A

A

A

W

P

K

G

G

T

T

G

G

A

D

A

C

K
|
K

C

M

G

G

N

N

Y
|
Y

A

G

S

S

L

L

V

F

A

A

Q

Q

A

C

E

E

A

A

I

V

R

D

E

N

G

G

C

C

D

Q

Q
|
Q

V

V

V

L

F

W

L

L

D

Y

A

G

V

-

E

E

R

D

R

H

W

Q

V

I

E

T

E
|
E

L

V

G

G

G
x
T

M
|
M

N
|
N

L

L

F

F

-

L

-

Y

F

W

V

I

F

N

D

E

D

D

G

G

-

E

S

E

M

L

V

A

T

T

P

P

L

L

G

D

G

G

T

I

I

I

L
|
L

P

P

G
|
G

I
x
V

T
|
T

R

R

E

R

S

C

I

I

L

L

T

D

L

L

A

A

R

H

E

Q

Q

W

G

G

-

E

I

F

T

K

V

V

R

S

E

E

E

R

P

Y

Y

L

A

T

I

M

D

D

Q

D

W

L

K

T

A

T

D

A

A

L

G

E

S

G

G

N

K

R

L

V

V

R

E

E

T

M

F

F

A

G

C

S

G
|
G

T
|
T

A
|
A

A

C

V

V

T

C

P

P

V

V

G

S

K

D

V

I

K

L

S

Y

R

K

D

G

G

E

E

T

F

I

T

H

I

I

G

P

S

T

-

M

-

E

G

N

G

G

P

P

G

K

Q

L

V

A

T

S

E

R

A

I

L

L

K

-

A

S

R

K

L

L

T

T

A

D

I

I

Q

Q

R

Y

G

G

Q

R

binding pyridoxal-5'-phosphate: R98 (= R99), R191 (= R191), K201 (= K201), Y206 (= Y206), E236 (= E237), T239 (≠ G240), M240 (= M241), N241 (= N242), L265 (= L262), G267 (= G264), V268 (≠ I265), T269 (= T266), G311 (= G307), T312 (= T308)
binding 3-(5-chloranyl-2,4-dimethoxy-phenyl)-6-(trifluoromethyl)-1H-pyrimidine-2,4-dione: F28 (= F29), Y172 (= Y173), Q223 (= Q223), T239 (≠ G240), A313 (= A309)

7nwbA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 1 (see paper)
41% identity, 90% coverage: 25:350/362 of query aligns to 24:355/365 of 7nwbA

query
sites
7nwbA

E

N

N

N

P

L

G

V

F
|
F

G

G

K

T

L

V

F

F

T

T

D

D

H

H

M

M

A

L

M

T

V

V

R

E

W

W

S

S

D

S

T

E

K

F

G

G

W

W

H

E

D

K

A

P

K

H

V

I

V

K

A

P

R

L

G

Q

P

N

L

L

S

S

L

L

D

H

P

P

A

G

T

S

A

S

V

A

L

L

H

H

Y
|
Y

A

A

Q

V

E

E

I

L

F
|
F

E

E

G

G

L

L

K

K

A

A

Y

F

R

R

T

G

G

V

D

D

E

N

G

K

T

I

A

R

L

L

F

F

R

Q

P

P

L

N

E

L

N

N

A

M

R

D

R
|
R

F

M

R

Y

E

R

S

S

A

A

Q

V

R

R

M

A

A

T

M

L

P

P

E

V

L

F

P

D

D

K

D

E

L

E

F

L

L

L

G

E

S

C

I

I

E

Q

A

Q

L

L

V

V

K

K

A

L

D

D

R

Q

E

E

W

W

I

V

P

P

Q

Y

I

S

E

T

G

S

G

A

S

S

L

L

Y
|
Y

L

I

R

R

P

P

F

T

M

F

F

I

A

G

S

T

E

E

V

P

F

S

L

L

G
|
G

V
|
V

K

K

P

K

A

P

S

T

E

K

Y

A

L

L

Y

L

L

F

V

V

I

L

A

L

S

S

P

P

A

V

G

G

A

P

Y
|
Y

F

F

K

S

G

S

G

G

A

T

-

F

P

N

A

P

V

V

T

S

I

L

W

W

V

A

S

N

D

P

H

K

Y

Y

T

V

R
|
R

A

A

A

W

P

K

G

G

T

T

G

G

A

D

A

C

K
|
K

C

M

G

G

N

N

Y
|
Y

A

G

S

S

L

L

V

F

A

A

Q
|
Q

A

C

E

E

A

A

I

V

R

D

E

N

G

G

C

C

D

Q

Q
|
Q

V

V

V

L

F

W

L

L

D

Y

A

G

V

-

E

E

R

D

R

H

W

Q

V

I

E

T

E
|
E

L

V

G

G

G
x
T

M
|
M

N

N

L

L

F

F

-

L

-

Y

F

W

V

I

F

N

D

E

D

D

G

G

-

E

S

E

M

L

V

A

T

T

P

P

L

L

G

D

G

G

T

I

I

I

L
|
L

P

P

G
|
G

I
x
V

T
|
T

R

R

E

R

S

C

I

I

L

L

T

D

L

L

A

A

R

H

E

Q

Q

W

G

G

-

E

I

F

T

K

V

V

R

S

E

E

E

R

P

Y

Y

L

A

T

I

M

D

D

Q

D

W

L

K

T

A

T

D

A

A

L

G

E

S

G

G

N

K

R

L

V

V

R

E

E

T

M

F

F

A

G

C

S

G
|
G

T
|
T

A
|
A

A

C

V

V

T

C

P

P

V

V

G

S

K

D

V

I

K

L

S

Y

R

K

D

G

G

E

E

T

F

I

T

H

I

I

G

P

S

T

-

M

-

E

G

N

G

G

P

P

G

K

Q

L

V

A

T

S

E

R

A

I

L

L

K

-

A

S

R

K

L

L

T

T

A

D

I

I

Q

Q

R

Y

G

G

Q

R

binding pyridoxal-5'-phosphate: R98 (= R99), R191 (= R191), K201 (= K201), Y206 (= Y206), E236 (= E237), T239 (≠ G240), M240 (= M241), L265 (= L262), G267 (= G264), V268 (≠ I265), T269 (= T266), G311 (= G307), T312 (= T308)
binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-5-fluoranyl-2-(2-methylphenoxy)benzenecarbonitrile: F28 (= F29), Y69 (= Y70), F74 (= F75), Y140 (= Y141), G153 (= G154), V154 (= V155), Y172 (= Y173), Q213 (= Q213), Q223 (= Q223), A313 (= A309)

7nwaA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and compound a (see paper)
41% identity, 90% coverage: 25:350/362 of query aligns to 24:355/365 of 7nwaA

query
sites
7nwaA

E

N

N

N

P

L

G

V

F

F

G

G

K

T

L

V

F

F

T

T

D

D

H

H

M

M

A

L

M

T

V

V

R

E

W

W

S

S

D

S

T

E

K

F

G

G

W

W

H

E

D

K

A

P

K

H

V

I

V

K

A

P

R

L

G

Q

P

N

L

L

S

S

L

L

D

H

P

P

A

G

T

S

A

S

V

A

L

L

H

H

Y

Y

A

A

Q

V

E

E

I

L

F

F

E

E

G

G

L

L

K

K

A

A

Y

F

R

R

T

G

G

V

D

D

E

N

G

K

T

I

A

R

L

L

F

F

R

Q

P

P

L

N

E

L

N

N

A

M

R

D

R
|
R

F

M

R

Y

E

R

S

S

A

A

Q

V

R

R

M

A

A

T

M

L

P

P

E

V

L

F

P

D

D

K

D

E

L

E

F

L

L

L

G

E

S

C

I

I

E

Q

A

Q

L

L

V

V

K

K

A

L

D

D

R

Q

E

E

W

W

I

V

P

P

Q

Y

I

S

E

T

G

S

G

A

S

S

L

L

Y

Y

L

I

R
|
R

P

P

F

T

M

F

F

I

A

G

S

T

E

E

V

P

F

S

L

L

G
|
G

V
|
V

K

K

P

K

A

P

S

T

E

K

Y

A

L

L

Y

L

L

F

V

V

I

L

A

L

S

S

P

P

A

V

G

G

A

P

Y
|
Y

F
|
F

K

S

G

S

G

G

A

T

-

F

P

N

A

P

V
|
V

T

S

I

L

W

W

V

A

S

N

D

P

H

K

Y

Y

T

V

R

R

A

A

A

W

P

K

G

G

T

T

G

G

A

D

A

C

K
|
K

C

M

G

G

N

N

Y
|
Y

A

G

S

S

L

L

V

F

A

A

Q

Q

A

C

E

E

A

A

I

V

R

D

E

N

G

G

C

C

D

Q

Q
|
Q

V

V

V

L

F

W

L

L

D

Y

A

G

V

-

E

E

R

D

R

H

W

Q

V

I

E

T

E
|
E

L
x
V

G

G

G
x
T

M
|
M

N
|
N

L

L

F

F

-

L

-

Y

F

W

V

I

F

N

D

E

D

D

G

G

-

E

S

E

M

L

V

A

T

T

P

P

L

L

G

D

G

G

T

I

I

I

L
|
L

P

P

G
|
G

I
x
V

T
|
T

R

R

E

R

S

C

I

I

L

L

T

D

L

L

A

A

R

H

E

Q

Q

W

G

G

-

E

I

F

T

K

V

V

R

S

E

E

E

R

P

Y

Y

L

A

T

I

M

D

D

Q

D

W

L

K

T

A

T

D

A

A

L

G

E

S

G

G

N

K

R

L

V

V

R

E

E

T

M

F

F

A

G

C

S

G
|
G

T
|
T

A
|
A

A

C

V

V

T
x
C

P

P

V

V

G

S

K

D

V

I

K

L

S

Y

R

K

D

G

G

E

E

T

F

I

T

H

I

I

G

P

S

T

-

M

-

E

G

N

G

G

P

P

G

K

Q

L

V

A

T

S

E

R

A

I

L

L

K

-

A

S

R

K

L

L

T

T

A

D

I

I

Q

Q

R

Y

G

G

Q

R

binding pyridoxal-5'-phosphate: R98 (= R99), K201 (= K201), Y206 (= Y206), E236 (= E237), T239 (≠ G240), N241 (= N242), L265 (= L262), G267 (= G264), V268 (≠ I265), T269 (= T266), T312 (= T308)
binding 2-[[4-chloranyl-2,6-bis(fluoranyl)phenyl]methylamino]-7-oxidanylidene-5-propyl-4H-pyrazolo[1,5-a]pyrimidine-3-carbonitrile: R142 (= R143), G153 (= G154), V154 (= V155), Y172 (= Y173), F173 (= F174), V181 (= V181), Q223 (= Q223), V237 (≠ L238), T239 (≠ G240), M240 (= M241), G311 (= G307), A313 (= A309), C317 (≠ T313)

2abjA Crystal structure of human branched chain amino acid transaminase in a complex with an inhibitor, c16h10n2o4f3scl, and pyridoxal 5' phosphate. (see paper)
41% identity, 90% coverage: 25:350/362 of query aligns to 25:356/366 of 2abjA

query
sites
2abjA

E

N

N

N

P

L

G

V

F
|
F

G

G

K

T

L

V

F

F

T

T

D

D

H

H

M

M

A

L

M

T

V

V

R

E

W

W

S

S

D

S

T

E

K

F

G

G

W

W

H

E

D

K

A

P

K

H

V

I

V

K

A

P

R

L

G

Q

P

N

L

L

S

S

L

L

D

H

P

P

A

G

T

S

A

S

V

A

L

L

H

H

Y

Y

A

A

Q

V

E

E

I

L

F
|
F

E

E

G

G

L

L

K

K

A

A

Y

F

R

R

T

G

G

V

D

D

E

N

G

K

T

I

A

R

L

L

F

F

R

Q

P

P

L

N

E

L

N

N

A

M

R

D

R
|
R

F

M

R

Y

E

R

S

S

A

A

Q

V

R

R

M

A

A

T

M

L

P

P

E

V

L

F

P

D

D

K

D

E

L

E

F

L

L

L

G

E

S

C

I

I

E

Q

A

Q

L

L

V

V

K

K

A

L

D

D

R

Q

E

E

W

W

I

V

P

P

Q

Y

I

S

E

T

G

S

G

A

S

S

L

L

Y
|
Y

L

I

R

R

P

P

F

A

M

F

F

I

A

G

S

T

E

E

V

P

F

S

L

L

G

G

V

V

K

K

P

K

A

P

S

T

E

K

Y

A

L

L

Y

L

L

F

V

V

I

L

A

L

S

S

P

P

A

V

G

G

A

P

Y
|
Y

F

F

K

S

G

S

G

G

A

T

-

F

P

N

A

P

V

V

T

S

I

L

W

W

V

A

S

N

D

P

H

K

Y

Y

T

V

R
|
R

A

A

A

W

P

K

G

G

T

T

G

G

A

D

A

C

K
|
K

C

M

G

G

N

N

Y
|
Y

A

G

S

S

L

L

V

F

A

A

Q

Q

A

C

E

E

A

D

I

V

R

D

E

N

G

G

C

C

D

Q

Q
|
Q

V

V

V

L

F

W

L

L

D

Y

A

G

V

R

E

D

R

H

R

Q

W

-

V

I

E

T

E
|
E

L

V

G

G

G
x
T

M
|
M

N
|
N

L

L

F

F

-

L

-

Y

F

W

V

I

F

N

D

E

D

D

G

G

-

E

S

E

M

L

V

A

T

T

P

P

L

L

G

D

G

G

T

I

I

I

L
|
L

P

P

G
|
G

I
x
V

T
|
T

R

R

E

R

S

C

I

I

L

L

T

D

L

L

A

A

R

H

E

Q

Q

W

G

G

-

E

I

F

T

K

V

V

R

S

E

E

E

R

P

Y

Y

L

A

T

I

M

D

D

Q

D

W

L

K

T

A

T

D

A

A

L

G

E

S

G

G

N

K

R

L

V

V

R

E

E

T

M

F

F

A

S

C

S

G
|
G

T
|
T

A
|
A

A
x
C

V

V

T

C

P

P

V

V

G

S

K

D

V

I

K

L

S

Y

R

K

D

G

G

E

E

T

F

I

T

H

I

I

G

P

S

T

-

M

-

E

G

N

G

G

P

P

G

K

Q

L

V

A

T

S

E

R

A

I

L

L

K

-

A

S

R

K

L

L

T

T

A

D

I

I

Q

Q

R

Y

G

G

Q

R

active site: K202 (= K201)
binding n'-(5-chlorobenzofuran-2-carbonyl)-2-(trifluoromethyl)benzenesulfonohydrazide: F29 (= F29), F75 (= F75), Y141 (= Y141), Y173 (= Y173), K202 (= K201), Q224 (= Q223), T240 (≠ G240), M241 (= M241), G312 (= G307), A314 (= A309), C315 (≠ A310)
binding pyridoxal-5'-phosphate: R99 (= R99), R192 (= R191), K202 (= K201), Y207 (= Y206), E237 (= E237), T240 (≠ G240), M241 (= M241), N242 (= N242), L266 (= L262), G268 (= G264), V269 (≠ I265), T270 (= T266), T313 (= T308)

7nxnA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor compound 21(5-f) (see paper)
41% identity, 90% coverage: 25:350/362 of query aligns to 24:352/362 of 7nxnA

query
sites
7nxnA

E

N

N

N

P

L

G

V

F
|
F

G

G

K

T

L

V

F

F

T

T

D

D

H

H

M

M

A

L

M

T

V

V

R

E

W

W

S

S

D

S

T

E

K

F

G

G

W

W

H

E

D

K

A

P

K

H

V

I

V

K

A

P

R

L

G

Q

P

N

L

L

S

S

L

L

D

H

P

P

A

G

T

S

A

S

V

A

L

L

H

H

Y
|
Y

A

A

Q

V

E

E

I

L

F
|
F

E

E

G

G

L

L

K

K

A

A

Y

F

R

R

T

G

G

V

D

D

E

N

G

K

T

I

A

R

L

L

F

F

R

Q

P

P

L

N

E

L

N

N

A

M

R

D

R
|
R

F

M

R

Y

E

R

S

S

A

A

Q

V

R

R

M

A

A

T

M

L

P

P

E

V

L

F

P

D

D

K

D

E

L

E

F

L

L

L

G

E

S

C

I

I

E

Q

A

Q

L

L

V

V

K

K

A

L

D

D

R

Q

E

E

W

W

I

V

P

P

Q

Y

I

S

E

T

G

S

G

A

S

S

L

L

Y

Y

L

I

R

R

P

P

F

T

M

F

F

I

A

G

S

T

E

E

V

P

F

S

L

L

G
|
G

V
|
V

K

K

P

K

A

P

S

T

E

K

Y

A

L

L

Y

L

L

F

V

V

I

L

A

L

S

S

P

P

A

V

G

G

A

P

Y
|
Y

F

F

K

S

G

F

G

N

A

-

P

-

A

P

V

V

T

S

I

L

W

W

V

A

S

N

D

P

H

K

Y

Y

T

V

R
|
R

A

A

A

W

P

K

G

G

T

T

G

G

A

D

A

C

K
|
K

C

M

G

G

N

N

Y
|
Y

A

G

S

S

L

L

V

F

A

A

Q
|
Q

A

C

E

E

A

A

I

V

R

D

E

N

G

G

C

C

D
x
Q

Q
|
Q

V

V

V

L

F

W

L

L

D

Y

A

G

V

-

E

E

R

D

R

H

W

Q

V

I

E

T

E
|
E

L

V

G

G

G
x
T

M
|
M

N
|
N

L

L

F

F

-

L

-

Y

F

W

V

I

F

N

D

E

D

D

G

G

-

E

S

E

M

L

V

A

T

T

P

P

L

L

G

D

G

G

T

I

I

I

L
|
L

P

P

G
|
G

I
x
V

T
|
T

R

R

E

R

S

C

I

I

L

L

T

D

L

L

A

A

R

H

E

Q

Q

W

G

G

-

E

I

F

T

K

V

V

R

S

E

E

E

R

P

Y

Y

L

A

T

I

M

D

D

Q

D

W

L

K

T

A

T

D

A

A

L

G

E

S

G

G

N

K

R

L

V

V

R

E

E

T

M

F

F

A

G

C

S

G

G

T
|
T

A

A

A

C

V

V

T

C

P

P

V

V

G

S

K

D

V

I

K

L

S

Y

R

K

D

G

G

E

E

T

F

I

T

H

I

I

G

P

S

T

-

M

-

E

G

N

G

G

P

P

G

K

Q

L

V

A

T

S

E

R

A

I

L

L

K

-

A

S

R

K

L

L

T

T

A

D

I

I

Q

Q

R

Y

G

G

Q

R

binding pyridoxal-5'-phosphate: R98 (= R99), R188 (= R191), K198 (= K201), Y203 (= Y206), E233 (= E237), T236 (≠ G240), M237 (= M241), N238 (= N242), L262 (= L262), G264 (= G264), V265 (≠ I265), T266 (= T266), T309 (= T308)
binding 4-[6-[bis(fluoranyl)-phenyl-methyl]-2,4-bis(oxidanylidene)-1~{H}-pyrimidin-3-yl]-5-fluoranyl-2-(2-methylphenoxy)benzenecarbonitrile: F28 (= F29), Y69 (= Y70), F74 (= F75), G153 (= G154), V154 (= V155), Y172 (= Y173), Q210 (= Q213), Q219 (≠ D222), Q220 (= Q223), T236 (≠ G240)

7ntrA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and substrate mimic 3-phenylpropionate (see paper)
41% identity, 90% coverage: 25:350/362 of query aligns to 24:352/362 of 7ntrA

query
sites
7ntrA

E

N

N

N

P

L

G

V

F
|
F

G

G

K

T

L

V

F

F

T

T

D

D

H

H

M

M

A

L

M

T

V

V

R

E

W

W

S

S

D

S

T

E

K

F

G

G

W

W

H

E

D

K

A

P

K

H

V

I

V

K

A

P

R

L

G

Q

P

N

L

L

S

S

L

L

D

H

P

P

A

G

T

S

A

S

V

A

L

L

H

H

Y
|
Y

A

A

Q

V

E

E

I

L

F

F

E

E

G

G

L

L

K

K

A

A

Y

F

R

R

T

G

G

V

D

D

E

N

G

K

T

I

A

R

L

L

F

F

R

Q

P

P

L

N

E

L

N

N

A

M

R

D

R
|
R

F

M

R

Y

E

R

S

S

A

A

Q

V

R

R

M

A

A

T

M

L

P

P

E

V

L

F

P

D

D

K

D

E

L

E

F

L

L

L

G

E

S

C

I

I

E

Q

A

Q

L

L

V

V

K

K

A

L

D

D

R

Q

E

E

W

W

I

V

P

P

Q

Y

I

S

E

T

G

S

G

A

S

S

L

L

Y

Y

L

I

R

R

P

P

F

T

M

F

F

I

A

G

S

T

E

E

V

P

F

S

L

L

G

G

V
|
V

K

K

P

K

A

P

S

T

E

K

Y

A

L

L

Y

L

L

F

V

V

I

L

A

L

S

S

P

P

A

V

G

G

A

P

Y

Y

F

F

K

S

G

F

G

N

A

-

P

-

A

P

V

V

T

S

I

L

W

W

V

A

S

N

D

P

H

K

Y

Y

T

V

R
|
R

A

A

A

W

P

K

G

G

T

T

G

G

A

D

A

C

K
|
K

C

M

G

G

N

N

Y
|
Y

A

G

S

S

L

L

V

F

A

A

Q

Q

A

C

E

E

A

A

I

V

R

D

E

N

G

G

C

C

D

Q

Q

Q

V

V

V

L

F

W

L

L

D

Y

A

G

V

-

E

E

R

D

R

H

W

Q

V

I

E

T

E
|
E

L

V

G

G

G
x
T

M
|
M

N

N

L

L

F

F

-

L

-

Y

F

W

V

I

F

N

D

E

D

D

G

G

-

E

S

E

M

L

V

A

T

T

P

P

L

L

G

D

G

G

T

I

I

I

L
|
L

P

P

G
|
G

I
x
V

T
|
T

R

R

E

R

S

C

I

I

L

L

T

D

L

L

A

A

R

H

E

Q

Q

W

G

G

-

E

I

F

T

K

V

V

R

S

E

E

E

R

P

Y

Y

L

A

T

I

M

D

D

Q

D

W

L

K

T

A

T

D

A

A

L

G

E

S

G

G

N

K

R

L

V

V

R

E

E

T

M

F

F

A

G

C

S

G
|
G

T
|
T

A
|
A

A

C

V

V

T

C

P

P

V

V

G

S

K

D

V

I

K

L

S

Y

R

K

D

G

G

E

E

T

F

I

T

H

I

I

G

P

S

T

-

M

-

E

G

N

G

G

P

P

G

K

Q

L

V

A

T

S

E

R

A

I

L

L

K

-

A

S

R

K

L

L

T

T

A

D

I

I

Q

Q

R

Y

G

G

Q

R

binding hydrocinnamic acid: F28 (= F29), Y69 (= Y70), V154 (= V155), T236 (≠ G240), G308 (= G307), T309 (= T308), A310 (= A309)
binding pyridoxal-5'-phosphate: R98 (= R99), R188 (= R191), K198 (= K201), Y203 (= Y206), E233 (= E237), T236 (≠ G240), M237 (= M241), L262 (= L262), G264 (= G264), V265 (≠ I265), T266 (= T266), G308 (= G307), T309 (= T308)

7nxoA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor compound 24(5-f) (see paper)
41% identity, 90% coverage: 25:350/362 of query aligns to 23:351/361 of 7nxoA

query
sites
7nxoA

E

N

N

N

P

L

G

V

F
|
F

G

G

K

T

L

V

F

F

T

T

D

D

H

H

M

M

A

L

M

T

V

V

R

E

W

W

S

S

D

S

T

E

K

F

G

G

W

W

H

E

D

K

A

P

K

H

V

I

V

K

A

P

R

L

G

Q

P

N

L

L

S

S

L

L

D

H

P

P

A

G

T

S

A

S

V

A

L

L

H

H

Y
|
Y

A

A

Q

V

E

E

I

L

F
|
F

E

E

G

G

L

L

K

K

A

A

Y

F

R

R

T

G

G

V

D

D

E

N

G

K

T

I

A

R

L

L

F

F

R

Q

P

P

L

N

E

L

N

N

A

M

R

D

R
|
R

F

M

R

Y

E

R

S

S

A

A

Q

V

R

R

M

A

A

T

M

L

P

P

E

V

L

F

P

D

D

K

D

E

L

E

F

L

L

L

G

E

S

C

I

I

E

Q

A

Q

L

L

V

V

K

K

A

L

D

D

R

Q

E

E

W

W

I

V

P

P

Q

Y

I

S

E

T

G

S

G

A

S

S

L

L

Y

Y

L

I

R

R

P

P

F

T

M

F

F

I

A

G

S

T

E

E

V

P

F

S

L

L

G
|
G

V
|
V

K

K

P

K

A

P

S

T

E

K

Y

A

L

L

Y

L

L

F

V

V

I

L

A

L

S

S

P

P

A

V

G

G

A

P

Y
|
Y

F
|
F

K

S

G

F

G

N

A

-

P

-

A

P

V

V

T

S

I

L

W

W

V

A

S

N

D

P

H

K

Y

Y

T

V

R

R

A

A

A

W

P

K

G

G

T

T

G

G

A

D

A

C

K
|
K

C

M

G

G

N

N

Y
|
Y

A

G

S

S

L

L

V

F

A

A

Q
|
Q

A

C

E

E

A

A

I

V

R

D

E

N

G

G

C

C

D
x
Q

Q
|
Q

V

V

V

L

F

W

L

L

D

Y

A

G

V

-

E

E

R

D

R

H

W

Q

V

I

E

T

E
|
E

L

V

G

G

G
x
T

M
|
M

N
|
N

L

L

F

F

-

L

-

Y

F

W

V

I

F

N

D

E

D

D

G

G

-

E

S

E

M

L

V

A

T

T

P

P

L

L

G

D

G

G

T

I

I

I

L
|
L

P

P

G
|
G

I
x
V

T
|
T

R

R

E

R

S

C

I

I

L

L

T

D

L

L

A

A

R

H

E

Q

Q

W

G

G

-

E

I

F

T

K

V

V

R

S

E

E

E

R

P

Y

Y

L

A

T

I

M

D

D

Q

D

W

L

K

T

A

T

D

A

A

L

G

E

S

G

G

N

K

R

L

V

V

R

E

E

T

M

F

F

A

G

C

S

G

G

T
|
T

A

A

A

C

V

V

T

C

P

P

V

V

G

S

K

D

V

I

K

L

S

Y

R

K

D

G

G

E

E

T

F

I

T

H

I

I

G

P

S

T

-

M

-

E

G

N

G

G

P

P

G

K

Q

L

V

A

T

S

E

R

A

I

L

L

K

-

A

S

R

K

L

L

T

T

A

D

I

I

Q

Q

R

Y

G

G

Q

R

binding pyridoxal-5'-phosphate: R97 (= R99), K197 (= K201), Y202 (= Y206), E232 (= E237), T235 (≠ G240), M236 (= M241), N237 (= N242), L261 (= L262), G263 (= G264), V264 (≠ I265), T265 (= T266), T308 (= T308)
binding 4-[2,4-bis(oxidanylidene)-6-(phenylsulfonyl)-1H-pyrimidin-3-yl]-5-fluoranyl-2-(2-methylphenoxy)benzenecarbonitrile: F27 (= F29), Y68 (= Y70), F73 (= F75), G152 (= G154), V153 (= V155), Y171 (= Y173), F172 (= F174), Q209 (= Q213), Q218 (≠ D222), Q219 (= Q223), T235 (≠ G240)

2coiA Crystal structure of oxidized human cytosolic branched-chain aminotransferase complexed with gabapentin (see paper)
41% identity, 90% coverage: 26:350/362 of query aligns to 24:349/358 of 2coiA

query
sites
2coiA

N

N

P

L

G

V

F

F

G

G

K

T

L

V

F

F

T

T

D

D

H

H

M

M

A

L

M

T

V

V

R

E

W

W

S

S

D

S

T

E

K

F

G

G

W

W

H

E

D

K

A

P

K

H

V

I

V

K

A

P

R

L

G

Q

P

N

L

L

S

S

L

L

D

H

P

P

A

G

T

S

A

S

V

A

L

L

H

H

Y
|
Y

A

A

Q

V

E

E

I

L

F

F

E

E

G

G

L

L

K

K

A

A

Y

F

R

R

T

G

G

V

D

D

E

N

G

K

T

I

A

R

L

L

F

F

R

Q

P

P

L

N

E

L

N

N

A

M

R

D

R
|
R

F

M

R

Y

E

R

S

S

A

A

Q

V

R

R

M

A

A

T

M

L

P

P

E

V

L

F

P

D

D

K

D

E

L

E

F

L

L

L

G

E

S

C

I

I

E

Q

A

Q

L

L

V

V

K

K

A

L

D

D

R

Q

E

E

W

W

I

V

P

P

Q

Y

I

S

E

T

G

S

G

A

S

S

L

L

Y

Y

L

I

R
|
R

P

P

F

T

M

F

F

I

A

G

S

T

E

E

V

P

F

S

L

L

G

G

V
|
V

K

K

P

K

A

P

S

T

E

K

Y

A

L

L

Y

L

L

F

V

V

I

L

A

L

S

S

P

P

A

V

G

G

A

P

Y

Y

F

F

K

N

G

-

A

-

P

-

A

P

V

V

T

S

I

L

W

W

V

A

S

N

D

P

H

K

Y

Y

T

V

R
|
R

A

A

A

W

P

K

G

G

T

T

G

G

A

D

A

C

K
|
K

C

M

G

G

N

N

Y
|
Y

A

G

S

S

L

L

V

F

A

A

Q

Q

A

C

E

E

A

A

I

V

R

D

E

N

G

G

C

C

D

Q

Q

Q

V

V

V

L

F

W

L

L

D

Y

A

G

V

-

E

E

R

D

R

H

W

Q

V

I

E

T

E
|
E

L

V

G

G

G
x
T

M

M

N

N

L

L

F

F

-

L

-

Y

F

W

V

I

F

N

D

E

D

D

G

G

-

E

S

E

M

L

V

A

T

T

P

P

L

L

G

D

G

G

T

I

I

I

L
|
L

P

P

G
|
G

I
x
V

T
|
T

R

R

E

R

S

C

I

I

L

L

T

D

L

L

A

A

R

H

E

Q

Q

W

G

G

-

E

I

F

T

K

V

V

R

S

E

E

E

R

P

Y

Y

L

A

T

I

M

D

D

Q

D

W

L

K

T

A

T

D

A

A

L

G

E

S

G

G

N

K

R

L

V

V

R

E

E

T

M

F

F

A

G

C

S

G
|
G

T
|
T

A
|
A

A

C

V

V

T

C

P

P

V

V

G

S

K

D

V

I

K

L

S

Y

R

K

D

G

G

E

E

T

F

I

T

H

I

I

G

P

S

T

-

M

-

E

G

N

G

G

P

P

G

K

Q

L

V

A

T

S

E

R

A

I

L

L

K

-

A

S

R

K

L

L

T

T

A

D

I

I

Q

Q

R

Y

G

G

Q

R

active site: K195 (= K201)
binding [1-(aminomethyl)cyclohexyl]acetic acid: Y68 (= Y70), R141 (= R143), V153 (= V155), T233 (≠ G240), G305 (= G307), T306 (= T308), A307 (= A309)
binding pyridoxal-5'-phosphate: R97 (= R99), R185 (= R191), K195 (= K201), Y200 (= Y206), E230 (= E237), T233 (≠ G240), L259 (= L262), G261 (= G264), V262 (≠ I265), T263 (= T266), T306 (= T308)

2cogA Crystal structure of oxidized human cytosolic branched-chain aminotransferase complexed with 4-methylvalerate (see paper)
41% identity, 90% coverage: 26:350/362 of query aligns to 24:349/358 of 2cogA

query
sites
2cogA

N

N

P

L

G

V

F

F

G

G

K

T

L

V

F

F

T

T

D

D

H

H

M

M

A

L

M

T

V

V

R

E

W

W

S

S

D

S

T

E

K

F

G

G

W

W

H

E

D

K

A

P

K

H

V

I

V

K

A

P

R

L

G

Q

P

N

L

L

S

S

L

L

D

H

P

P

A

G

T

S

A

S

V

A

L

L

H

H

Y

Y

A

A

Q

V

E

E

I

L

F

F

E

E

G

G

L

L

K

K

A

A

Y

F

R

R

T

G

G

V

D

D

E

N

G

K

T

I

A

R

L

L

F

F

R

Q

P

P

L

N

E

L

N

N

A

M

R

D

R
|
R

F

M

R

Y

E

R

S

S

A

A

Q

V

R

R

M

A

A

T

M

L

P

P

E

V

L

F

P

D

D

K

D

E

L

E

F

L

L

L

G

E

S

C

I

I

E

Q

A

Q

L

L

V

V

K

K

A

L

D

D

R

Q

E

E

W

W

I

V

P

P

Q

Y

I

S

E

T

G

S

G

A

S

S

L

L

Y

Y

L

I

R

R

P

P

F

T

M

F

F

I

A

G

S

T

E

E

V

P

F

S

L

L

G

G

V

V

K

K

P

K

A

P

S

T

E

K

Y

A

L

L

Y

L

L

F

V

V

I

L

A

L

S

S

P

P

A

V

G

G

A

P

Y
|
Y

F

F

K

N

G

-

A

-

P

-

A

P

V

V

T

S

I

L

W

W

V

A

S

N

D

P

H

K

Y

Y

T

V

R

R

A

A

A

W

P

K

G

G

T

T

G

G

A

D

A

C

K
|
K

C

M

G

G

N

N

Y
|
Y

A

G

S

S

L

L

V

F

A

A

Q

Q

A

C

E

E

A

A

I

V

R

D

E

N

G

G

C

C

D

Q

Q

Q

V

V

V

L

F

W

L

L

D

Y

A

G

V

-

E

E

R

D

R

H

W

Q

V

I

E

T

E
|
E

L

V

G

G

G
x
T

M

M

N

N

L

L

F

F

-

L

-

Y

F

W

V

I

F

N

D

E

D

D

G

G

-

E

S

E

M

L

V

A

T

T

P

P

L

L

G

D

G

G

T

I

I

I

L
|
L

P

P

G
|
G

I
x
V

T
|
T

R

R

E

R

S

C

I

I

L

L

T

D

L

L

A

A

R

H

E

Q

Q

W

G

G

-

E

I

F

T

K

V

V

R

S

E

E

E

R

P

Y

Y

L

A

T

I

M

D

D

Q

D

W

L

K

T

A

T

D

A

A

L

G

E

S

G

G

N

K

R

L

V

V

R

E

E

T

M

F

F

A

G

C

S

G

G

T
|
T

A
|
A

A

C

V

V

T

C

P

P

V

V

G

S

K

D

V

I

K

L

S

Y

R

K

D

G

G

E

E

T

F

I

T

H

I

I

G

P

S

T

-

M

-

E

G

N

G

G

P

P

G

K

Q

L

V

A

T

S

E

R

A

I

L

L

K

-

A

S

R

K

L

L

T

T

A

D

I

I

Q

Q

R

Y

G

G

Q

R

active site: K195 (= K201)
binding 4-methyl valeric acid: Y171 (= Y173), T233 (≠ G240), T306 (= T308), A307 (= A309)
binding pyridoxal-5'-phosphate: R97 (= R99), K195 (= K201), Y200 (= Y206), E230 (= E237), T233 (≠ G240), L259 (= L262), G261 (= G264), V262 (≠ I265), T263 (= T266), T306 (= T308)

Query Sequence

>Ga0059261_3811 FitnessBrowser__Korea:Ga0059261_3811
VSSAMFEFEANPNPVPASERAALLENPGFGKLFTDHMAMVRWSDTKGWHDAKVVARGPLS
LDPATAVLHYAQEIFEGLKAYRTGDEGTALFRPLENARRFRESAQRMAMPELPDDLFLGS
IEALVKADREWIPQIEGGSLYLRPFMFASEVFLGVKPASEYLYLVIASPAGAYFKGGAPA
VTIWVSDHYTRAAPGGTGAAKCGGNYASSLVAQAEAIREGCDQVVFLDAVERRWVEELGG
MNLFFVFDDGSMVTPPLGGTILPGITRESILTLAREQGITVREEPYAIDQWKADAGSGKL
VETFACGTAAVVTPVGKVKSRDGEFTIGSGGPGQVTEALKARLTAIQRGQAPDIHGWVHR
FG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory