SitesBLAST
Comparing Ga0059261_4132 Ga0059261_4132 succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71) to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5u0mA Fatty aldehyde dehydrogenase from marinobacter aquaeolei vt8 and cofactor complex (see paper)
51% identity, 96% coverage: 5:457/471 of query aligns to 20:473/488 of 5u0mA
- active site: N148 (= N133), K171 (= K156), E246 (= E230), C280 (= C263), E377 (= E361), P455 (= P439)
- binding nicotinamide-adenine-dinucleotide: F144 (≠ L129), Y147 (= Y132), N148 (= N133), K171 (= K156), S173 (= S158), E174 (= E159), G207 (= G192), T222 (= T206), G223 (= G207), S224 (= S208), V227 (≠ A211), E246 (= E230), M247 (≠ L231), G248 (= G232), C280 (= C263), E377 (= E361), F379 (= F363)
5u0lA X-ray crystal structure of fatty aldehyde dehydrogenase enzymes from marinobacter aquaeolei vt8 complexed with a substrate (see paper)
51% identity, 96% coverage: 5:457/471 of query aligns to 20:473/488 of 5u0lA
- active site: N148 (= N133), K171 (= K156), E246 (= E230), C280 (= C263), E377 (= E361), P455 (= P439)
- binding decanal: K107 (= K92), H152 (= H137), L153 (= L138), G156 (= G141), H157 (= H142), S456 (= S440), A457 (= A441)
3ju8A Crystal structure of succinylglutamic semialdehyde dehydrogenase from pseudomonas aeruginosa.
53% identity, 97% coverage: 7:461/471 of query aligns to 21:477/486 of 3ju8A
- active site: N147 (= N133), K170 (= K156), E245 (= E230), C279 (= C263), E377 (= E361), P455 (= P439)
- binding nicotinamide-adenine-dinucleotide: G144 (= G130), Y146 (= Y132), N147 (= N133), L152 (= L138), K170 (= K156), S172 (= S158), F220 (= F205), T221 (= T206), G222 (= G207), S223 (= S208), T226 (≠ A211), E245 (= E230), M246 (≠ L231), G247 (= G232), C279 (= C263), E377 (= E361), F379 (= F363), F444 (= F428)
4cazA Crystal structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa in complex with nadh
33% identity, 93% coverage: 3:440/471 of query aligns to 21:464/489 of 4cazA
- active site: N152 (= N133), K175 (= K156), E251 (= E230), C285 (= C263), E386 (= E361), E463 (≠ P439)
- binding [[(2R,3S,4R,5R)-5-[(3R)-3-aminocarbonyl-3,4-dihydro-2H-pyridin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanidyl-phosphoryl] [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl phosphate: I148 (≠ L129), G149 (= G130), W151 (≠ Y132), N152 (= N133), K175 (= K156), E178 (= E159), G208 (= G188), G212 (= G192), F226 (= F205), T227 (= T206), G228 (= G207), G229 (≠ S208), T232 (≠ A211), V236 (≠ F215), E251 (= E230), L252 (= L231), C285 (= C263), E386 (= E361), F388 (= F363)
2woxA Betaine aldehyde dehydrogenase from pseudomonas aeruginosa with NAD(p) h-catalytic thiol adduct. (see paper)
33% identity, 93% coverage: 3:440/471 of query aligns to 21:464/489 of 2woxA
- active site: N152 (= N133), K175 (= K156), E251 (= E230), C285 (= C263), E386 (= E361), E463 (≠ P439)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I148 (≠ L129), G149 (= G130), W151 (≠ Y132), N152 (= N133), K175 (= K156), S177 (= S158), E178 (= E159), G208 (= G188), G212 (= G192), F226 (= F205), T227 (= T206), G228 (= G207), G229 (≠ S208), T232 (≠ A211), V236 (≠ F215), E251 (= E230), L252 (= L231), C285 (= C263), E386 (= E361), F388 (= F363)
2wmeA Crystallographic structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa (see paper)
33% identity, 93% coverage: 3:440/471 of query aligns to 21:464/489 of 2wmeA
- active site: N152 (= N133), K175 (= K156), E251 (= E230), C285 (= C263), E386 (= E361), E463 (≠ P439)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G149 (= G130), W151 (≠ Y132), K175 (= K156), S177 (= S158), E178 (= E159), G208 (= G188), G212 (= G192), F226 (= F205), G228 (= G207), G229 (≠ S208), T232 (≠ A211), V236 (≠ F215)
Q9HTJ1 NAD/NADP-dependent betaine aldehyde dehydrogenase; BADH; EC 1.2.1.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
33% identity, 93% coverage: 3:440/471 of query aligns to 22:465/490 of Q9HTJ1
- GAWN 150:153 (≠ GPYN 130:133) binding
- K162 (≠ H142) active site, Charge relay system
- KPSE 176:179 (= KPSE 156:159) binding
- G209 (= G188) binding
- GTST 230:233 (≠ SAGA 208:211) binding
- E252 (= E230) active site, Proton acceptor
- C286 (= C263) binding covalent; modified: Cysteine sulfenic acid (-SOH)
- E387 (= E361) binding
- E464 (≠ P439) active site, Charge relay system
P17202 Aminoaldehyde dehydrogenase BADH; 4-trimethylammoniobutyraldehyde dehydrogenase BADH; Aminobutyraldehyde dehydrogenase BADH; Betaine aldehyde dehydrogenase; SoBADH; EC 1.2.1.-; EC 1.2.1.47; EC 1.2.1.19; EC 1.2.1.8 from Spinacia oleracea (Spinach) (see 3 papers)
32% identity, 91% coverage: 9:438/471 of query aligns to 29:466/497 of P17202
- D96 (≠ E74) binding
- SPW 156:158 (≠ GPY 130:132) binding
- Y160 (≠ F134) mutation to A: Decreases binding affinity for betaine aldehyde; increases binding affinity for 4-(trimethylamino)butanal.
- W167 (≠ G141) mutation to A: Decreases binding affinity for betaine aldehyde; increases binding affinity for 4-aminobutanal.
- KPSE 182:185 (= KPSE 156:159) binding
- L186 (≠ E160) binding
- SSAT 236:239 (≠ SAGA 208:211) binding
- V251 (vs. gap) binding in other chain
- L258 (= L231) binding
- W285 (≠ I257) mutation to A: Decreases binding affinity for betaine aldehyde.
- E390 (= E361) binding
- A441 (≠ V412) mutation to I: Decreases binding affinity for betaine aldehyde; increases binding affinity for 4-aminobutanal.
- C450 (≠ T419) mutation to S: Loss of partial inactivation by betaine aldehyde in the absence of NAD(+).
- W456 (≠ F428) binding ; mutation to A: Decreases binding affinity for betaine aldehyde.
- K460 (≠ G432) binding
Sites not aligning to the query:
6wsbA Crystal structure of a betaine aldehyde dehydrogenase from burkholderia pseudomallei bound to cofactor NAD (see paper)
33% identity, 93% coverage: 5:440/471 of query aligns to 23:463/489 of 6wsbA
- active site: N152 (= N133), E250 (= E230), C284 (= C263), E462 (≠ P439)
- binding nicotinamide-adenine-dinucleotide: I148 (≠ L129), G149 (= G130), A150 (≠ P131), W151 (≠ Y132), N152 (= N133), K175 (= K156), E178 (= E159), G208 (≠ R189), G211 (= G192), A212 (= A193), F225 (= F205), T226 (= T206), G227 (= G207), G228 (≠ S208), T231 (≠ A211), V235 (≠ F215), E250 (= E230), L251 (= L231), G252 (= G232), C284 (= C263), E385 (= E361), F387 (= F363)
8vr1A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (ctp bound)
33% identity, 93% coverage: 5:440/471 of query aligns to 22:463/488 of 8vr1A
8vr0A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (gmp bound)
33% identity, 93% coverage: 5:440/471 of query aligns to 22:463/488 of 8vr0A
8vqzA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (cmp bound)
33% identity, 93% coverage: 5:440/471 of query aligns to 22:463/488 of 8vqzA
8vqwC Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (coa bound)
33% identity, 93% coverage: 5:440/471 of query aligns to 22:463/488 of 8vqwC
- binding coenzyme a: I147 (≠ L129), W150 (≠ Y132), K174 (= K156), S176 (= S158), E177 (= E159), G207 (= G188), G211 (= G192), F225 (= F205), G227 (= G207), G228 (≠ S208), S231 (≠ A211), H331 (≠ A312), F387 (= F363)
8vj3A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (fad bound)
33% identity, 93% coverage: 5:440/471 of query aligns to 22:463/488 of 8vj3A
8uzoA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (adp bound)
33% identity, 93% coverage: 5:440/471 of query aligns to 22:463/488 of 8uzoA
8uznA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (amp bound)
33% identity, 93% coverage: 5:440/471 of query aligns to 22:463/488 of 8uznA
- binding adenosine monophosphate: I147 (≠ L129), G148 (= G130), K174 (= K156), S176 (= S158), E177 (= E159), G207 (= G188), G211 (= G192), F225 (= F205), G228 (≠ S208), S231 (≠ A211), K234 (≠ H214)
8uzmA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (NADPH bound)
33% identity, 93% coverage: 5:440/471 of query aligns to 22:463/488 of 8uzmA
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G148 (= G130), W150 (≠ Y132), K174 (= K156), S176 (= S158), E177 (= E159), G207 (= G188), G211 (= G192), F225 (= F205), T226 (= T206), G227 (= G207), G228 (≠ S208), S231 (≠ A211), E250 (= E230), G252 (= G232), C284 (= C263), E385 (= E361), F387 (= F363)
8uzkA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (NADP+ bound)
33% identity, 93% coverage: 5:440/471 of query aligns to 22:463/488 of 8uzkA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I147 (≠ L129), G148 (= G130), W150 (≠ Y132), N151 (= N133), K174 (= K156), S176 (= S158), E177 (= E159), G207 (= G188), G211 (= G192), F225 (= F205), G227 (= G207), G228 (≠ S208), S231 (≠ A211), E250 (= E230), F387 (= F363)
8skfA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (lattice translocation disorder)
33% identity, 93% coverage: 5:440/471 of query aligns to 31:472/497 of 8skfA
- binding calcium ion: T33 (≠ S7), I34 (≠ T8), D100 (≠ E74), V187 (≠ E160)
- binding nicotinamide-adenine-dinucleotide: I156 (≠ L129), G157 (= G130), A158 (≠ P131), W159 (≠ Y132), K183 (= K156), E186 (= E159), G216 (= G188), G220 (= G192), T235 (= T206), G236 (= G207), G237 (≠ S208), S240 (≠ A211), K243 (≠ H214), E259 (= E230), C293 (= C263), F396 (= F363)
Q59931 NADP-dependent glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase [NADP(+)]; Non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase; Triosephosphate dehydrogenase; EC 1.2.1.9 from Streptococcus mutans serotype c (strain ATCC 700610 / UA159) (see 3 papers)
30% identity, 90% coverage: 10:435/471 of query aligns to 25:451/475 of Q59931
- R103 (vs. gap) binding
- S151 (≠ G130) binding
- K177 (= K156) binding
- T180 (≠ E159) binding
- D215 (≠ A193) binding
- 230:251 (vs. 207:231, 36% identical) binding
- E377 (= E361) binding
- R437 (≠ G421) binding
Query Sequence
>Ga0059261_4132 Ga0059261_4132 succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71)
MSALFASTNPATGETLWEGPVASAEDCARAVERARTAFPAWAAQSPDARAAILTRYAAVL
GERKDALAEAIARETGKPLWETATEVASMIGKVAISIEAMAARAGTRESAMPFGRAVLAH
RPHGVMAVLGPYNFPGHLPNGHIVPALLAGNTLVFKPSEETPLVGQLMVEALHAAGIPED
VAILLQGGRETGAALVSQDIDGLLFTGSAGAGMHFRRSFAERPAVILALELGGNNPLVAW
DGEPEAVASIVVASTFITTGQRCSCARRLIVPEGAAGDAIVDAVAALSDRLRIGRWDETP
EPFMGPLVSTGAAERAAAQVAALVGLGAREIRPFGGVEGRSGAFVRPAILDVTGLEVPDE
EIFAPVLQVRRVADFDAALAAANQTRFGLAAGLISDDDALWARFQAQARAGVVNRNRPTT
GAASSMPFGGLGDSGNHRPSAYYAADYCAYPVASLEAERIADQLGGLRGIG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory