SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_00426 FitnessBrowser__Burk376:H281DRAFT_00426 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P04983 Ribose import ATP-binding protein RbsA; EC 7.5.2.7 from Escherichia coli (strain K12) (see paper)
50% identity, 99% coverage: 1:497/503 of query aligns to 1:494/501 of P04983

query
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P04983

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K43 (= K43) mutation to R: Loss of transport.

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
29% identity, 44% coverage: 3:221/503 of query aligns to 1:215/241 of 4u00A

query
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4u00A

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binding adenosine-5'-diphosphate: F12 (= F14), V17 (≠ A19), S37 (≠ N39), G38 (= G40), S39 (≠ A41), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
34% identity, 47% coverage: 5:240/503 of query aligns to 12:250/265 of P07821

query
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P07821

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K50 (= K43) mutation to Q: Lack of activity.
D172 (= D167) mutation to E: Lack of activity.
E173 (= E168) mutation to A: Lack of activity.

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
33% identity, 44% coverage: 1:221/503 of query aligns to 1:222/648 of P75831

query
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P75831

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K47 (= K43) mutation to L: Lack of activity.
D169 (= D167) mutation to N: Lack of activity.

3c4jA Abc protein artp in complex with atp-gamma-s
30% identity, 43% coverage: 4:221/503 of query aligns to 3:216/242 of 3c4jA

query
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3c4jA

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|
K

S
|
S

T
|
T

L

F

M

L

K

R

I

C

L

L

A

N

G

L

V

L

Y

E

T

D

R

F

D

D

S

E

G

G

E

E

I

I

L

I

L

I

G

D

G

G

-

I

-

N

-

L

-

K

-

A

Q

K

P

D

V

T

E

N

L

L

Q

N

S

K

P

V

R

R

D

E

A

E

Q

-

A

-

A

-

G

-

I

V

G

G

I

M

I

V

H

F

Q

Q

E

R

L

F

Q

N

L

L

M

F

N

P

H

H

L

M

T

T

V

V

A

L

Q

N

N

N

I

I

F

T

I

L

G

A

-

P

-

M

-

K

-

V

-

R

R

K

E

W

P

P

R

R

G

-

R

-

L

-

G

-

L

-

F

-

L

-

D

-

E

-

D

E

K

K

L

A

N

E

A

A

K

K

A

A

R

M

E

E

I

L

L

L

S

D

R

K

M

V

H

G

V

L

N

K

I

D

D

K

P

A

R

H

A

A

M

Y

V

P

G

D

N

S

L

L

T

S

V

G

A

G

S

Q

Q

A

Q

Q

M

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

A

F

M

D

E

S

P

R

K

V

I

L

M

I

L

M

F

D

D

E

E

P

P

T

T

S

S

A

A

L

L

N

D

D

P

A

E

E

M

I

V

A

G

E

E

L

V

F

L

R

S

I

V

I

M

R

K

E

Q

L

L

K

A

Q

N

R

E

G

G

V

M

G

T

V

M

V

V

Y

V

I

V

S

T

H

H

K

E

M

M

D

G

E

F

L

A

K

R

Q

E

I

V

A

G

D

D

R

R

V

V

T

L

V

F

L

M

R

-

D

D

G

G

E

G

Y

Y

V

I

binding phosphothiophosphoric acid-adenylate ester: F13 (= F14), V18 (≠ A19), S38 (≠ N39), G39 (= G40), S40 (≠ A41), G41 (= G42), K42 (= K43), S43 (= S44), T44 (= T45)

3c41J Abc protein artp in complex with amp-pnp/mg2+
30% identity, 43% coverage: 4:221/503 of query aligns to 3:216/242 of 3c41J

query
sites
3c41J

L

M

I

I

S

D

V

V

K

H

K

Q

L

L

S

K

K

K

S

S

F
|
F

P

G

G

S

V

L

R

E

A
x
V

L

L

H

K

D

G

V

I

Q

N

F

V

D

H

L

I

V

R

E

E

G

G

E

E

V

V

H

V

A

V

L

V

M

I

G

G

E

P

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

L

K

R

I

C

L

L

A

N

G

L

V

L

Y

E

T

D

R

F

D

D

S

E

G

G

E

E

I

I

L

I

L

I

G

D

G

G

-

I

-

N

-

L

-

K

-

A

Q

K

P

D

V

T

E

N

L

L

Q

N

S

K

P

V

R

R

D

E

A

E

Q

-

A

-

A

-

G

-

I

V

G

G

I

M

I

V

H

F

Q

Q

E

R

L

F

Q

N

L

L

M

F

N

P

H

H

L

M

T

T

V

V

A

L

Q

N

N

N

I

I

F

T

I

L

G

A

-

P

-

M

-

K

-

V

-

R

R

K

E

W

P

P

R

R

G

-

R

-

L

-

G

-

L

-

F

-

L

-

D

-

E

-

D

E

K

K

L

A

N

E

A

A

K

K

A

A

R

M

E

E

I

L

L

L

S

D

R

K

M

V

H

G

V

L

N

K

I

D

D

K

P

A

R

H

A

A

M

Y

V

P

G

D

N

S

L

L

T

S

V

G

A

G

S

Q

Q

A

Q

Q

M

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

A

F

M

D

E

S

P

R

K

V

I

L

M

I

L

M

F

D
|
D

E

E

P

P

T

T

S

S

A

A

L

L

N

D

D

P

A

E

E

M

I

V

A

G

E

E

L

V

F

L

R

S

I

V

I

M

R

K

E

Q

L

L

K

A

Q

N

R

E

G

G

V

M

G

T

V

M

V

V

Y

V

I

V

S

T

H

H

K

E

M

M

D

G

E

F

L

A

K

R

Q

E

I

V

A

G

D

D

R

R

V

V

T

L

V

F

L

M

R

-

D

D

G

G

E

G

Y

Y

V

I

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F14), V18 (≠ A19), S38 (≠ N39), G39 (= G40), G41 (= G42), K42 (= K43), S43 (= S44), T44 (= T45)
binding magnesium ion: S43 (= S44), D163 (= D167)

2olkA Abc protein artp in complex with adp-beta-s
30% identity, 43% coverage: 4:221/503 of query aligns to 3:216/242 of 2olkA

query
sites
2olkA

L

M

I

I

S

D

V

V

K

H

K

Q

L

L

S

K

K

K

S

S

F
|
F

P

G

G

S

V

L

R

E

A
x
V

L

L

H

K

D

G

V

I

Q

N

F

V

D

H

L

I

V

R

E

E

G

G

E

E

V

V

H

V

A

V

L

V

M

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

L

K

R

I

C

L

L

A

N

G

L

V

L

Y

E

T

D

R

F

D

D

S

E

G

G

E

E

I

I

L

I

L

I

G

D

G

G

-

I

-

N

-

L

-

K

-

A

Q

K

P

D

V

T

E

N

L

L

Q

N

S

K

P

V

R

R

D

E

A

E

Q

-

A

-

A

-

G

-

I

V

G

G

I

M

I

V

H

F

Q

Q

E

R

L

F

Q

N

L

L

M

F

N

P

H

H

L

M

T

T

V

V

A

L

Q

N

N

N

I

I

F

T

I

L

G

A

-

P

-

M

-

K

-

V

-

R

R

K

E

W

P

P

R

R

G

-

R

-

L

-

G

-

L

-

F

-

L

-

D

-

E

-

D

E

K

K

L

A

N

E

A

A

K

K

A

A

R

M

E

E

I

L

L

L

S

D

R

K

M

V

H

G

V

L

N

K

I

D

D

K

P

A

R

H

A

A

M

Y

V

P

G

D

N

S

L

L

T

S

V

G

A

G

S

Q

Q

A

Q

Q

M

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

A

F

M

D

E

S

P

R

K

V

I

L

M

I

L

M

F

D

D

E

E

P

P

T

T

S

S

A

A

L

L

N

D

D

P

A

E

E

M

I

V

A

G

E

E

L

V

F

L

R

S

I

V

I

M

R

K

E

Q

L

L

K

A

Q

N

R

E

G

G

V

M

G

T

V

M

V

V

Y

V

I

V

S

T

H

H

K

E

M

M

D

G

E

F

L

A

K

R

Q

E

I

V

A

G

D

D

R

R

V

V

T

L

V

F

L

M

R

-

D

D

G

G

E

G

Y

Y

V

I

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (= F14), V18 (≠ A19), S38 (≠ N39), G39 (= G40), S40 (≠ A41), G41 (= G42), K42 (= K43), S43 (= S44), T44 (= T45)

2oljA Abc protein artp in complex with adp/mg2+
30% identity, 43% coverage: 4:221/503 of query aligns to 3:216/242 of 2oljA

query
sites
2oljA

L

M

I

I

S

D

V

V

K

H

K

Q

L

L

S

K

K

K

S

S

F
|
F

P

G

G

S

V

L

R

E

A
x
V

L

L

H

K

D

G

V

I

Q

N

F

V

D

H

L

I

V

R

E

E

G

G

E

E

V

V

H

V

A

V

L

V

M

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

L

K

R

I

C

L

L

A

N

G

L

V

L

Y

E

T

D

R

F

D

D

S

E

G

G

E

E

I

I

L

I

L

I

G

D

G

G

-

I

-

N

-

L

-

K

-

A

Q

K

P

D

V

T

E

N

L

L

Q

N

S

K

P

V

R

R

D

E

A

E

Q

-

A

-

A

-

G

-

I

V

G

G

I

M

I

V

H

F

Q

Q

E

R

L

F

Q

N

L

L

M

F

N

P

H

H

L

M

T

T

V

V

A

L

Q

N

N

N

I

I

F

T

I

L

G

A

-

P

-

M

-

K

-

V

-

R

R

K

E

W

P

P

R

R

G

-

R

-

L

-

G

-

L

-

F

-

L

-

D

-

E

-

D

E

K

K

L

A

N

E

A

A

K

K

A

A

R

M

E

E

I

L

L

L

S

D

R

K

M

V

H

G

V

L

N

K

I

D

D

K

P

A

R

H

A

A

M

Y

V

P

G

D

N

S

L

L

T

S

V

G

A

G

S

Q

Q

A

Q

Q

M

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

A

F

M

D

E

S

P

R

K

V

I

L

M

I

L

M

F

D

D

E

E

P

P

T

T

S

S

A

A

L

L

N

D

D

P

A

E

E

M

I

V

A

G

E

E

L

V

F

L

R

S

I

V

I

M

R

K

E

Q

L

L

K

A

Q

N

R

E

G

G

V

M

G

T

V

M

V

V

Y

V

I

V

S

T

H

H

K

E

M

M

D

G

E

F

L

A

K

R

Q

E

I

V

A

G

D

D

R

R

V

V

T

L

V

F

L

M

R

-

D

D

G

G

E

G

Y

Y

V

I

binding adenosine-5'-diphosphate: F13 (= F14), V18 (≠ A19), S38 (≠ N39), G39 (= G40), S40 (≠ A41), G41 (= G42), K42 (= K43), S43 (= S44), T44 (= T45)

6mhzA Vanadate trapped cryo-em structure of e.Coli lptb2fg transporter (see paper)
29% identity, 43% coverage: 5:222/503 of query aligns to 3:216/235 of 6mhzA

query
sites
6mhzA

I

L

S

T

V

A

K

K

K

N

L

L

S

A

K

K

S

A

F
x
Y

P

K

G

G

V

R

R

R

A

V

L

V

H

E

D

D

V

V

Q

S

F

L

D

T

L

V

V

N

E

S

G

G

E

E

V

I

H

V

A

G

L

L

M

L

G

G

E

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

M

F

K

Y

I

M

L

V

A

V

G

G

V

I

Y

V

T

P

R

R

D

D

S

A

G

G

E

N

I

I

L

I

L

I

G

D

G

D

Q

D

P

D

V

I

E

S

L

L

Q

L

S

-

P

P

R

L

D

H

A

A

Q

R

A

A

-

R

A

R

G

G

I

I

G

G

I

Y

I

L

H

P

Q
|
Q

E

E

L

A

Q

S

L

I

M

F

N

R

H

R

L

L

T

S

V

V

A

Y

Q

D

N

N

I

L

F

M

I

A

G

V

R

L

E

Q

P

I

R

R

G

D

R

D

L

L

G

S

L

A

F

E

L

Q

D

R

E

E

D

D

K

R

L

A

N

N

A

-

K

-

A

-

R

-

E

E

I

L

L

M

S

E

R

E

M

F

H

H

V

I

N

E

I

H

D

L

P

R

R

D

A

S

M

M

V

G

G

Q

N
x
S

L

L

T
x
S

V
x
G

A
x
G

S

E

Q

R

Q

R

M

R

V

V

E

E

I

I

A

A

K

R

A

A

L

L

S

A

F

A

D

N

S

P

R

K

V

F

L

I

I

L

M

L

D

D

E
|
E

P

P

T

F

S

A

A
x
G

L

V

N

D

D

P

A

I

E

S

I

V

A

I

E

D

L

I

F

K

R

R

I

I

I

I

R

E

E

H

L

L

K

R

Q

D

R

S

G

G

V

L

G

G

V

V

V

L

Y

I

I

T

S

D

H
|
H

K

N

M

V

D

R

E

E

L

T

K

L

Q

A

I

V

A

C

D

E

R

R

V

A

T

Y

V

I

L

V

R

S

D

Q

G

G

E

H

Y

L

V

I

A

A

binding adp orthovanadate: Y12 (≠ F14), N37 (= N39), G38 (= G40), G40 (= G42), K41 (= K43), T42 (≠ S44), T43 (= T45), Q84 (= Q86), S136 (≠ N142), S138 (≠ T144), G139 (≠ V145), G140 (≠ A146), E162 (= E168), G166 (≠ A172), H194 (= H200)

6b8bA E. Coli lptb in complex with adp and a novobiocin derivative (see paper)
29% identity, 43% coverage: 5:222/503 of query aligns to 3:216/233 of 6b8bA

query
sites
6b8bA

I

L

S

T

V

A

K

K

K

N

L

L

S

A

K

K

S

A

F
x
Y

P

K

G

G

V
x
R

R

R

A
x
V

L

V

H

E

D

D

V

V

Q

S

F

L

D

T

L

V

V

N

E

S

G

G

E

E

V

I

H

V

A

G

L

L

M

L

G

G

E

P

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

M

F

K

Y

I

M

L

V

A

V

G

G

V

I

Y

V

T

P

R

R

D

D

S

A

G

G

E

N

I

I

L

I

L

I

G

D

G

D

Q

D

P

D

V

I

E

S

L

L

Q

L

S

-

P

P

R

L

D

H

A

A

Q

R

A

A

-

R

A

R

G

G

I

I

G

G

I

Y

I

L

H

P

Q

Q

E

E

L

A

Q

S

L

I

M
x
F

N
x
R

H
x
R

L

L

T

S

V

V

A

Y

Q

D

N

N

I

L

F

M

I

A

G

V

R

L

E

Q

P

I

R

R

G

D

R

D

L

L

G

S

L

A

F

E

L

Q

D

R

E

E

D

D

K

R

L

A

N

N

A

-

K

-

A

-

R

-

E

E

I

L

L

M

S

E

R

E

M

F

H

H

V

I

N

E

I

H

D

L

P

R

R

D

A

S

M

M

V

G

G

Q

N

S

L

L

T

S

V

G

A

G

S

E

Q

R

Q

R

M

R

V

V

E

E

I

I

A

A

K

R

A

A

L

L

S

A

F

A

D

N

S

P

R

K

V

F

L

I

I

L

M

L

D

D

E

E

P

P

T

F

S

A

A

G

L

V

N

D

D

P

A

I

E

S

I

V

A

I

E

D

L

I

F

K

R

R

I

I

I

I

R

E

E

H

L

L

K

R

Q

D

R

S

G

G

V

L

G

G

V

V

V

L

Y

I

I

T

S

D

H

H

K

N

M

V

D

R

E

E

L

T

K

L

Q

A

I

V

A

C

D

E

R

R

V

A

T

Y

V

I

L

V

R

S

D

Q

G

G

E

H

Y

L

V

I

A

A

binding adenosine-5'-diphosphate: Y12 (≠ F14), R15 (≠ V17), V17 (≠ A19), G38 (= G40), G40 (= G42), K41 (= K43), T42 (≠ S44), T43 (= T45)
binding (3s,5s,7s)-N-{7-[(3-O-carbamoyl-6-deoxy-5-methyl-4-O-methyl-beta-D-gulopyranosyl)oxy]-4-hydroxy-8-methyl-2-oxo-2H-1-benzopyran-3-yl}tricyclo[3.3.1.1~3,7~]decane-1-carboxamide: F89 (≠ M91), R90 (≠ N92), R91 (≠ H93)

6s8nB Cryo-em structure of lptb2fgc in complex with lipopolysaccharide (see paper)
29% identity, 43% coverage: 5:222/503 of query aligns to 3:216/238 of 6s8nB

query
sites
6s8nB

I

L

S

T

V

A

K

K

K

N

L

L

S

A

K

K

S

A

F

Y

P

K

G

G

V

R

R

R

A

V

L

V

H

E

D

D

V

V

Q

S

F

L

D

T

L

V

V

N

E

S

G

G

E

E

V

I

H

V

A

G

L

L

M

L

G

G

E

P

N

N

G

G

A

A

G

G

K

K

S

T

T

T

L

T

M

F

K

Y

I

M

L

V

A

V

G

G

V

I

Y

V

T

P

R

R

D

D

S

A

G

G

E

N

I

I

L

I

L

I

G

D

G

D

Q

D

P

D

V

I

E

S

L

L

Q

L

S

-

P

P

R

L

D

H

A

A

Q

R

A

A

-

R

A

R

G

G

I

I

G

G

I

Y

I

L

H

P

Q

Q

E

E

L

A

Q

S

L

I

M

F

N
x
R

H

R

L

L

T

S

V

V

A

Y

Q

D

N

N

I

L

F

M

I

A

G

V

R

L

E

Q

P

I

R

R

G

D

R

D

L

L

G

S

L

A

F

E

L

Q

D

R

E

E

D

D

K

R

L

A

N

N

A

-

K

-

A

-

R

-

E

E

I

L

L

M

S

E

R

E

M

F

H

H

V

I

N

E

I

H

D

L

P

R

R

D

A

S

M
|
M

V
x
G

G
x
Q

N

S

L

L

T

S

V

G

A

G

S

E

Q

R

Q

R

M

R

V

V

E

E

I

I

A

A

K

R

A

A

L

L

S

A

F

A

D

N

S

P

R

K

V

F

L

I

I

L

M

L

D

D

E

E

P

P

T

F

S

A

A

G

L

V

N

D

D

P

A

I

E

S

I

V

A

I

E

D

L

I

F

K

R

R

I

I

I

I

R

E

E

H

L

L

K

R

Q

D

R

S

G

G

V

L

G

G

V

V

V

L

Y

I

I

T

S

D

H

H

K

N

M

V

D

R

E

E

L

T

K

L

Q

A

I

V

A

C

D

E

R

R

V

A

T

Y

V

I

L

V

R

S

D

Q

G

G

E

H

Y

L

V

I

A

A

binding 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione: R90 (≠ N92), M133 (= M139), G134 (≠ V140), Q135 (≠ G141)

6s8gA Cryo-em structure of lptb2fgc in complex with amp-pnp (see paper)
29% identity, 43% coverage: 5:222/503 of query aligns to 3:216/238 of 6s8gA

query
sites
6s8gA

I

L

S

T

V

A

K

K

K

N

L

L

S

A

K

K

S

A

F
x
Y

P

K

G

G

V
x
R

R

R

A

V

L

V

H

E

D

D

V

V

Q

S

F

L

D

T

L

V

V

N

E

S

G

G

E

E

V

I

H

V

A

G

L

L

M

L

G

G

E

P

N
|
N

G

G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

M

F

K

Y

I

M

L

V

A

V

G

G

V

I

Y

V

T

P

R

R

D

D

S

A

G

G

E

N

I

I

L

I

L

I

G

D

G

D

Q

D

P

D

V

I

E

S

L

L

Q

L

S

-

P

P

R

L

D

H

A

A

Q

R

A

A

-

R

A

R

G

G

I

I

G

G

I

Y

I

L

H

P

Q
|
Q

E

E

L

A

Q

S

L

I

M

F

N

R

H

R

L

L

T

S

V

V

A

Y

Q

D

N

N

I

L

F

M

I

A

G

V

R

L

E

Q

P

I

R

R

G

D

R

D

L

L

G

S

L

A

F

E

L

Q

D

R

E

E

D

D

K

R

L

A

N

N

A

-

K

-

A

-

R

-

E

E

I

L

L

M

S

E

R

E

M

F

H

H

V

I

N

E

I

H

D

L

P

R

R

D

A

S

M

M

V

G

G

Q

N
x
S

L

L

T
x
S

V

G

A

G

S
x
E

Q

R

Q

R

M

R

V

V

E

E

I

I

A

A

K

R

A

A

L

L

S

A

F

A

D

N

S

P

R

K

V

F

L

I

I

L

M

L

D

D

E

E

P

P

T

F

S

A

A

G

L

V

N

D

D

P

A

I

E

S

I

V

A

I

E

D

L

I

F

K

R

R

I

I

I

I

R

E

E

H

L

L

K

R

Q

D

R

S

G

G

V

L

G

G

V

V

V

L

Y

I

I

T

S

D

H

H

K

N

M

V

D

R

E

E

L

T

K

L

Q

A

I

V

A

C

D

E

R

R

V

A

T

Y

V

I

L

V

R

S

D

Q

G

G

E

H

Y

L

V

I

A

A

binding phosphoaminophosphonic acid-adenylate ester: Y12 (≠ F14), R15 (≠ V17), N37 (= N39), G40 (= G42), K41 (= K43), T42 (≠ S44), T43 (= T45), Q84 (= Q86), S136 (≠ N142), S138 (≠ T144), E141 (≠ S147)

6b89A E. Coli lptb in complex with adp and novobiocin (see paper)
29% identity, 43% coverage: 5:222/503 of query aligns to 3:216/234 of 6b89A

query
sites
6b89A

I

L

S

T

V

A

K

K

K

N

L

L

S

A

K

K

S

A

F
x
Y

P

K

G

G

V
x
R

R

R

A
x
V

L

V

H

E

D

D

V

V

Q

S

F

L

D

T

L

V

V

N

E

S

G

G

E

E

V

I

H

V

A

G

L

L

M

L

G

G

E

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

M

F

K

Y

I

M

L

V

A

V

G

G

V

I

Y

V

T

P

R

R

D

D

S

A

G

G

E

N

I

I

L

I

L

I

G

D

G

D

Q

D

P

D

V

I

E

S

L

L

Q

L

S

-

P

P

R
x
L

D
x
H

A

A

Q

R

A

A

-

R

A

R

G

G

I

I

G

G

I

Y

I

L

H
x
P

Q
|
Q

E

E

L
x
A

Q
x
S

L

I

M
x
F

N
x
R

H
x
R

L
|
L

T

S

V

V

A

Y

Q

D

N

N

I

L

F

M

I

A

G
x
V

R

L

E

Q

P

I

R

R

G

D

R

D

L

L

G

S

L

A

F

E

L

Q

D

R

E

E

D

D

K

R

L

A

N

N

A

-

K

-

A

-

R

-

E

E

I

L

L

M

S

E

R

E

M

F

H

H

V

I

N

E

I

H

D

L

P

R

R

D

A

S

M

M

V

G

G
x
Q

N

S

L

L

T

S

V

G

A

G

S

E

Q

R

Q

R

M

R

V

V

E

E

I

I

A

A

K
x
R

A

A

L

L

S

A

F

A

D

N

S

P

R

K

V

F

L

I

I

L

M

L

D

D

E

E

P

P

T

F

S

A

A

G

L

V

N

D

D

P

A

I

E

S

I

V

A

I

E

D

L

I

F

K

R

R

I

I

I

I

R

E

E

H

L

L

K

R

Q

D

R

S

G

G

V

L

G

G

V

V

V

L

Y

I

I

T

S

D

H

H

K

N

M

V

D

R

E

E

L

T

K

L

Q

A

I

V

A

C

D

E

R

R

V

A

T

Y

V

I

L

V

R

S

D

Q

G

G

E

H

Y

L

V

I

A

A

binding adenosine-5'-diphosphate: Y12 (≠ F14), R15 (≠ V17), V17 (≠ A19), N37 (= N39), G38 (= G40), G40 (= G42), K41 (= K43), T42 (≠ S44), T43 (= T45)
binding magnesium ion: T42 (≠ S44), Q84 (= Q86)
binding novobiocin: L71 (≠ R74), H72 (≠ D75), P83 (≠ H85), A86 (≠ L88), S87 (≠ Q89), F89 (≠ M91), R90 (≠ N92), R91 (≠ H93), L92 (= L94), V101 (≠ G103), Q135 (≠ G141), R149 (≠ K155)

4p31A Crystal structure of a selenomethionine derivative of e. Coli lptb in complex with adp-magensium (see paper)
29% identity, 43% coverage: 5:222/503 of query aligns to 3:216/234 of 4p31A

query
sites
4p31A

I

L

S

T

V

A

K

K

K

N

L

L

S

A

K

K

S

A

F
x
Y

P

K

G

G

V
x
R

R

R

A
x
V

L

V

H

E

D

D

V

V

Q

S

F

L

D

T

L

V

V

N

E

S

G

G

E

E

V

I

H

V

A

G

L

L

M

L

G

G

E

P

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

M

F

K

Y

I

M

L

V

A

V

G

G

V

I

Y

V

T

P

R

R

D

D

S

A

G

G

E

N

I

I

L

I

L

I

G

D

G

D

Q

D

P

D

V

I

E

S

L

L

Q

L

S

-

P

P

R

L

D

H

A

A

Q

R

A

A

-

R

A

R

G

G

I

I

G

G

I

Y

I

L

H

P

Q
|
Q

E

E

L

A

Q

S

L

I

M

F

N

R

H

R

L

L

T

S

V

V

A

Y

Q

D

N

N

I

L

F

M

I

A

G

V

R

L

E

Q

P

I

R

R

G

D

R

D

L

L

G

S

L

A

F

E

L

Q

D

R

E

E

D

D

K

R

L

A

N

N

A

-

K

-

A

-

R

-

E

E

I

L

L

M

S

E

R

E

M

F

H

H

V

I

N

E

I

H

D

L

P

R

R

D

A

S

M

M

V

G

G

Q

N

S

L

L

T

S

V

G

A

G

S

E

Q

R

Q

R

M

R

V

V

E

E

I

I

A

A

K

R

A

A

L

L

S

A

F

A

D

N

S

P

R

K

V

F

L

I

I

L

M

L

D

D

E

E

P

P

T

F

S

A

A

G

L

V

N

D

D

P

A

I

E

S

I

V

A

I

E

D

L

I

F

K

R

R

I

I

I

I

R

E

E

H

L

L

K

R

Q

D

R

S

G

G

V

L

G

G

V

V

V

L

Y

I

I

T

S

D

H

H

K

N

M

V

D

R

E

E

L

T

K

L

Q

A

I

V

A

C

D

E

R

R

V

A

T

Y

V

I

L

V

R

S

D

Q

G

G

E

H

Y

L

V

I

A

A

binding adenosine-5'-diphosphate: Y12 (≠ F14), R15 (≠ V17), V17 (≠ A19), G38 (= G40), G40 (= G42), K41 (= K43), T42 (≠ S44), T43 (= T45)
binding magnesium ion: T42 (≠ S44), Q84 (= Q86)

4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
28% identity, 55% coverage: 1:278/503 of query aligns to 1:273/285 of 4yerA

query
sites
4yerA

M

M

T

E

P

D

L

I

I

I

S

V

V

V

K

E

K

N

L

L

S

V

K

K

S

K

F
|
F

P

G

G

D

V
x
F

R

E

A

A

L

V

H

K

D

G

V

V

Q

S

F

F

D

S

L

V

V

K

E

K

G

G

E

E

V

I

H

F

A

A

L

F

M

L

G

G

E

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

M

I

K

H

I

M

L

L

A

T

G

T

V

L

Y

L

T

K

R

P

D

T

S

S

G

G

E

K

I

A

L

W

L

V

G

A

G

G

Q

H

P

D

V

V

E

-

L

L

Q

K

S

E

P

P

R

R

D

E

A

V

Q

R

A

R

A

K

G

-

I

I

G

G

I

I

I

V

H

F

Q

Q

E

D

L

Q

Q

S

L

L

M

D

N

R

H

E

L

L

T

T

V

A

A

Y

Q

E

N

N

I

M

F

Y

I

I

G

H

R

G

E

K

P

I

R

Y

G

G

R

Y

L

G

G

G

L

-

F

-

L

-

D

-

E

-

D

E

K

K

L

L

N

K

A

K

K

R

A

I

R

L

E

E

I

L

L

L

S

E

R

F

M

V

H

E

V

L

N

L

I

E

D

F

P

K

R

D

A

K

M

P

V

V

G

K

N
x
T

L
x
F

T
x
S

V

G

A

G

S

M

Q

A

Q

R

M

R

V

L

E

E

I

I

A

A

K

R

A

S

L

L

S

I

F

H

D

E

S

P

R

E

V

V

L

L

I

F

M

L

D

D

E

E

P

P

T

T

S

I

A

G

L

L

N

D

D

P

A

H

E

T

I

R

A

A

E

H

L

M

F

W

R

E

I

Y

I

I

R

S

E

K

L

M

K

K

Q

K

R

E

G

H

V

N

G

M

V

T

V

I

Y

F

I

L

-

T

S

T

H

H

K

Y

M

M

D

D

E

E

L

A

K

E

Q

Q

I

L

A

A

D

D

R

R

V

V

T

A

V

I

L

I

R

D

D

H

G

G

E

K

Y

I

V

I

A

A

T

L

V

G

A

T

A

P

A

T

D

E

T

-

S

-

V

-

E

-

A

-

I

-

I

L

G

K

M

R

M

M

V

V

G

G

R

K

T

E

L

I

-

I

-

Y

-

V

S

R

D

D

V

F

A

I

P

E

A

S

G

C

R

R

A

K

A

L

S

P

Q

D

G

G

E

R

I

L

A

E

L

L

E

N

V

V

R

E

N

D

L

I

-

E

-

E

-

I

-

T

H

Y

A

H

G

K

P

P

L

T

V

L

R

N

D

D

V

V

S

F

F

L

T

H

L

L

binding adenosine-5'-diphosphate: F14 (= F14), F17 (≠ V17), N39 (= N39), G40 (= G40), G42 (= G42), K43 (= K43), T44 (≠ S44), T45 (= T45), T135 (≠ N142), F136 (≠ L143), S137 (≠ T144)

5x40A Structure of a cbio dimer bound with amppcp (see paper)
33% identity, 45% coverage: 1:226/503 of query aligns to 1:224/280 of 5x40A

query
sites
5x40A

M

M

T

T

P

P

L

I

I

L

S

A

V

A

K

E

K

A

L

L

S

T

K

Y

S

A

F
|
F

P

P

G

G

-

G

V
|
V

R

K

A
|
A

L

L

H

D

D

D

V

L

Q

S

F

L

D

A

L

V

V

P

E

K

G

G

E

E

V

S

H

L

A

A

L

I

M

L

G

G

E

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

K

L

I

H

L

L

A

N

G

G

V

T

Y

L

T

R

R

P

D

Q

S

S

G

G

E

R

I

V

L

L

L

L

G

G

G

G

Q

T

P

A

V

T

E

G

L

-

Q

H

S

S

P

R

R

K

D

D

A

L

Q

T

A

G

A

W

-

R

-

R

G

R

I

V

G

G

I

L

I

V

H

L

Q
|
Q

E

D

L

A

-

D

-

D

Q

Q

L

L

M

F

N

A

H

T

L

-

T

T

V

V

A

F

Q

E

N

D

I

V

F

S

I

F

G

-

R

-

E

-

P

-

R

-

G

G

R

P

L

L

G

N

L

L

F

G

L

L

D

S

E

E

D

A

K

E

L

A

N

R

A

A

K

R

A

V

R

E

E

E

I

A

L

L

S

A

R

A

M

L

H

S

V

I

N

S

I

-

D

D

P

L

R

R

A

D

M

R

V

P

G

T

N
x
H

L
x
M

T
x
L

V
x
S

A

G

S
x
G

Q
|
Q

Q

K

-

R

M

R

V

V

E

A

I

I

A

A

K

G

A

A

L

V

S

A

F

M

D

R

S

P

R

E

V

V

L

L

I

L

M

L

D

D

E
x
Q

P

P

T

T

S

A

A

G

L

L

N

D

D

L

A

A

E

G

I

T

A

E

E

Q

L

L

F

L

R

T

I

L

I

L

R

R

E

G

L

L

K

R

Q

A

R

A

G

G

V

M

G

T

V

L

V

V

Y

F

I

S

S

T

H
|
H

K

D

M

V

D

E

E

L

L

A

K

A

Q

A

I

L

A

A

D

D

R

R

V

V

T

A

V

L

L

F

R

R

D

T

G

G

E

R

Y

V

V

L

A

A

T

E

V

G

A

A

A

A

binding phosphomethylphosphonic acid adenylate ester: F14 (= F14), V18 (= V17), A20 (= A19), N40 (= N39), G41 (= G40), G43 (= G42), K44 (= K43), S45 (= S44), T46 (= T45), Q88 (= Q86), H139 (≠ N142), M140 (≠ L143), L141 (≠ T144), S142 (≠ V145), G144 (≠ S147), Q145 (= Q148), Q166 (≠ E168), H198 (= H200)
binding magnesium ion: S45 (= S44), Q88 (= Q86)

6mbnA Lptb e163q in complex with atp (see paper)
29% identity, 43% coverage: 5:222/503 of query aligns to 4:217/241 of 6mbnA

query
sites
6mbnA

I

L

S

T

V

A

K

K

K

N

L

L

S

A

K

K

S

A

F
x
Y

P

K

G

G

V
x
R

R

R

A
x
V

L

V

H

E

D

D

V

V

Q

S

F

L

D

T

L

V

V

N

E

S

G

G

E

E

V

I

H

V

A

G

L

L

M

L

G

G

E

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

M

F

K

Y

I

M

L

V

A

V

G

G

V

I

Y

V

T

P

R

R

D

D

S

A

G

G

E

N

I

I

L

I

L

I

G

D

G

D

Q

D

P

D

V

I

E

S

L

L

Q

L

S

-

P

P

R

L

D

H

A

A

Q

R

A

A

-

R

A

R

G

G

I

I

G

G

I

Y

I

L

H

P

Q

Q

E

E

L

A

Q

S

L

I

M

F

N

R

H

R

L

L

T

S

V

V

A

Y

Q

D

N

N

I

L

F

M

I

A

G

V

R

L

E

Q

P

I

R

R

G

D

R

D

L

L

G

S

L

A

F

E

L

Q

D

R

E

E

D

D

K

R

L

A

N

N

A

-

K

-

A

-

R

-

E

E

I

L

L

M

S

E

R

E

M

F

H

H

V

I

N

E

I

H

D

L

P

R

R

D

A

S

M

M

V

G

G

Q

N

S

L

L

T

S

V

G

A

G

S

E

Q

R

Q

R

M

R

V

V

E

E

I

I

A

A

K

R

A

A

L

L

S

A

F

A

D

N

S

P

R

K

V

F

L

I

I

L

M

L

D

D

E

Q

P

P

T

F

S

A

A

G

L

V

N

D

D

P

A

I

E

S

I

V

A

I

E

D

L

I

F

K

R

R

I

I

I

I

R

E

E

H

L

L

K

R

Q

D

R

S

G

G

V

L

G

G

V

V

V

L

Y

I

I

T

S

D

H
|
H

K

N

M

V

D

R

E

E

L

T

K

L

Q

A

I

V

A

C

D

E

R

R

V

A

T

Y

V

I

L

V

R

S

D

Q

G

G

E

H

Y

L

V

I

A

A

binding adenosine-5'-triphosphate: Y13 (≠ F14), R16 (≠ V17), V18 (≠ A19), N38 (= N39), G39 (= G40), G41 (= G42), K42 (= K43), T43 (≠ S44), T44 (= T45), H195 (= H200)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
29% identity, 43% coverage: 4:221/503 of query aligns to 1:220/343 of P30750

query
sites
P30750

L

M

I

I

S

K

V

L

K

S

K

N

L

I

S

T

K

K

S

V

F

F

P

H

-

Q

-

G

-

T

-

R

G

T

V

I

R

Q

A

A

L

L

H

N

D

N

V

V

Q

S

F

L

D

H

L

V

V

P

E

A

G

G

E

Q

V

I

H

Y

A

G

L

V

M

I

G

G

E

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

I

K

R

I

C

L

V

A

N

G

L

V

L

Y

E

T

R

R

P

D

T

S

E

G

G

E

S

I

V

L

L

L

V

G

D

G

G

Q

Q

P

E

V

L

E

T

L

T

Q

L

S

S

P

E

R

S

D

E

A

L

Q

T

A

K

A

A

-

R

-

R

G

Q

I

I

G

G

I

M

I

I

H

F

Q

Q

E

H

L

F

Q

N

L

L

M

L

N

S

H

S

L

R

T

T

V

V

A

F

Q

G

N

N

I

V

F

-

I

-

G

-

R

-

E

-

P

-

R

-

G

-

R

A

L

L

G

P

L

L

F

E

L

L

D

D

-

N

-

T

-

P

E

K

D

D

K

E

L

V

N

K

A

R

K

R

A

V

R

T

E

E

I

L

L

L

S

S

R

L

M

V

H

G

V

L

N

G

I

D

D

K

P

H

R

D

A

S

M

Y

V

P

G

S

N

N

L

L

T

S

V

G

A

G

S

Q

Q

K

Q

Q

M

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

A

F

S

D

N

S

P

R

K

V

V

L

L

I

L

M

C

D

D

E
|
E

P

A

T

T

S

S

A

A

L

L

N

D

D

P

A

A

E

T

I

T

A

R

E

S

L

I

F

L

R

E

I

L

I

L

R

K

E

D

L

I

K

N

Q

R

R

R

-

L

G

G

V

L

G

T

V

I

V

L

Y

L

I

I

S

T

H

H

K

E

M

M

D

D

E

V

L

V

K

K

Q

R

I

I

A

C

D

D

R

C

V

V

T

A

V

V

L

I

R

S

D

N

G

G

E

E

Y

L

V

I

40:46 (vs. 39:45, 86% identical) binding
E166 (= E168) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
29% identity, 43% coverage: 4:221/503 of query aligns to 2:221/344 of 6cvlD

query
sites
6cvlD

L

M

I

I

S

K

V

L

K

S

K

N

L

I

S

T

K

K

S

V

F
|
F

P

H

-
x
Q

-

G

-

T

-

R

G

T

V
x
I

R

Q

A

A

L

L

H

N

D

N

V

V

Q

S

F

L

D

H

L

V

V

P

E

A

G

G

E

Q

V

I

H

Y

A

G

L

V

M

I

G

G

E

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

I

K

R