SitesBLAST

P40875 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain P-1) (see 3 papers)
25% identity, 47% coverage: 210:414/432 of query aligns to 188:375/405 of P40875

query
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P40875

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C195 (= C217) mutation to S: No change in activity.
C351 (≠ V390) mutation to A: No change in activity.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
30 G→A: Prevents covalent attachment of FMN. Blocks subunit assembly.
146 C→S: No change in activity.
173 modified: Tele-8alpha-FMN histidine; H→N: Prevents covalent attachment of FMN. Loss of activity. The mutant is considerably less stable than wild-type enzyme, the beta and delta subunits are lost during purification, which yields a stable alpha-gamma complex.
175 H→A: No effect on FMN binding and activity.

S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
26% identity, 46% coverage: 234:430/432 of query aligns to 183:363/369 of S5FMM4

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S5FMM4

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S202 (≠ G258) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
I332 (≠ W397) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
M342 (≠ I407) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.

Sites not aligning to the query:

51 G→S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
54 A→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
81 K→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.

4x9mA Oxidized l-alpha-glycerophosphate oxidase from mycoplasma pneumoniae with fad bound (see paper)
39% identity, 11% coverage: 2:47/432 of query aligns to 5:50/384 of 4x9mA

query
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4x9mA

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binding flavin-adenine dinucleotide: G10 (= G7), G12 (= G9), I14 (≠ A11), E33 (= E30), K34 (≠ R31), E41 (≠ G38), T42 (≠ A39), S43 (≠ T40), A45 (≠ G42), N46 (≠ H43), S47 (≠ G44), V49 (≠ T46)
binding sn-glycerol-3-phosphate: S47 (≠ G44)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 51, 176, 177, 209, 210, 212, 234, 319, 320, 346, 347, 348, 349, 350, 351, 352
binding sn-glycerol-3-phosphate: 51, 258, 259, 320, 348

1vrqB Crystal structure of heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with folinic acid (see paper)
25% identity, 52% coverage: 210:432/432 of query aligns to 187:389/402 of 1vrqB

query
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1vrqB

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active site: G326 (= G366), K358 (≠ G398)
binding n,n-dimethylglycine: K358 (≠ G398)
binding flavin-adenine dinucleotide: V196 (= V219), A224 (= A257), G225 (= G258), H228 (≠ G261), L247 (≠ V280), G353 (= G393), T354 (≠ P394), G355 (≠ A395), G356 (= G396), F357 (≠ W397), K358 (≠ G398)
binding flavin mononucleotide: V251 (= V284), E279 (≠ R315), R322 (≠ L362), W324 (= W364)

Sites not aligning to the query:

active site: 62, 65, 66
binding n,n-dimethylglycine: 65, 67, 69, 401
binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 51, 52, 53, 59, 60, 61, 63, 64, 65, 67
binding flavin mononucleotide: 62, 63, 172

P75063 Glycerol 3-phosphate oxidase; GlpO; L-alpha-glycerophosphate oxidase; EC 1.1.3.21 from Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1) (Mycoplasmoides pneumoniae) (see paper)
39% identity, 11% coverage: 2:47/432 of query aligns to 5:50/384 of P75063

query
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P75063

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I14 (≠ A11) binding
E33 (= E30) binding
TS 42:43 (≠ AT 39:40) binding
SGV 47:49 (≠ GGT 44:46) binding

Sites not aligning to the query:

177 binding
346:347 binding
352 binding

3ad8B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with pyrrole 2-carboxylate (see paper)
25% identity, 52% coverage: 210:432/432 of query aligns to 187:389/404 of 3ad8B

query
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3ad8B

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active site: G326 (= G366), K358 (≠ G398)
binding flavin-adenine dinucleotide: V196 (= V219), G225 (= G258), A226 (≠ G259), H228 (≠ G261), L247 (≠ V280), G353 (= G393), T354 (≠ P394), G355 (≠ A395), G356 (= G396), F357 (≠ W397), K358 (≠ G398)
binding flavin mononucleotide: V251 (= V284), E279 (≠ R315), R322 (≠ L362), W324 (= W364)
binding pyrrole-2-carboxylate: M264 (≠ I298), Y271 (≠ G306), T354 (≠ P394), K358 (≠ G398)

Sites not aligning to the query:

active site: 62, 65, 66
binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 52, 53, 59, 60, 61, 63, 64, 65, 67
binding flavin mononucleotide: 62, 63, 172
binding pyrrole-2-carboxylate: 65, 67, 69, 402

3ad7B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with methylthio acetate (see paper)
25% identity, 52% coverage: 210:432/432 of query aligns to 187:389/404 of 3ad7B

query
sites
3ad7B

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A

V

L

A

A

G
|
G

G
x
A

Y

G

G
x
H

S

S

L

S

A

V

L

L

L

A

E

E

R

L

L

A

G

G

L

F

A

E

L

L

P

P

L

I

H

Q

P

S

L

H

R

P

V
x
L

H

Q

T

A

L

L

V
|
V

A

S

P

E

I

L

A

-

H

F

E

E

E

P

C

V

A

H

P

P

H

T

V

V

A

V

I
x
M

V

S

D

N

A

H

I

I

K

-

R

H

I

V

G
x
Y

I

V

S

S

R

Q

M

A

N

-

H

H

R
x
K

L

G

R
x
E

I

L

A

V

G

M

G

G

A

A

V

G

L

I

Q

D

G

S

A

Y

N

N

L

G

I

Y

D

G

K

Q

P

R

L

G

G

A

E

F

A

H

L

V

T

I

K

E

E

E

A

Q

L

M

A

A

L

-

G

-

Q

-

A

A

T

A

H

V

D

E

W

L

I

F

P

P

G

I

A

F

A

A

R

R

I

A

S

H

A

V

A

L

L
x
R

P

T

W
|
W

E

G

G
|
G

I

I

K

V

L

D

L

T

S

T

P

M

D

D

G

A

L

S

P

P

V

I

G

S

N

K

A

T

L

P

H

I

P

Q

R

N

L

L

F

Y

V

V

N

N

V

C

G

G

H

W

G
|
G

P
x
T

A
x
G

G
|
G

W
x
F

G
x
K

L

G

A

T

C

P

G

G

A

A

G

G

K

Y

L

T

I

L

A

A

D

H

L

T

V

I

A

-

G

-

Q

-

A

A

P

H

D

D

L

E

P

P

D

H

E

K

T

L

L

N

A

A

A

P

L

F

R

A

A

L

D

E

R

R

F

F

R

E

active site: G326 (= G366), K358 (≠ G398)
binding flavin-adenine dinucleotide: V196 (= V219), G225 (= G258), A226 (≠ G259), H228 (≠ G261), L247 (≠ V280), G353 (= G393), T354 (≠ P394), G355 (≠ A395), G356 (= G396), F357 (≠ W397), K358 (≠ G398)
binding flavin mononucleotide: V251 (= V284), K277 (≠ R313), E279 (≠ R315), R322 (≠ L362), W324 (= W364)
binding [methylthio]acetate: M264 (≠ I298), Y271 (≠ G306), T354 (≠ P394), K358 (≠ G398)

Sites not aligning to the query:

active site: 62, 65, 66
binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 52, 53, 59, 60, 61, 63, 64, 65, 67
binding flavin mononucleotide: 62, 63, 172
binding [methylthio]acetate: 67, 69, 402

Q50LF2 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain U-96) (see 4 papers)
25% identity, 52% coverage: 210:432/432 of query aligns to 188:390/405 of Q50LF2

query
sites
Q50LF2

G

G

V

V

Q

D

F

I

M

I

L

Q

G

N

C

C

E

E

V
|
V

S

T

A

G

I

F

R

L

L

K

D

D

G

G

Q

E

R

K

A

V

A

T

V

G

E

V

L

K

A

T

P

T

R

R

P

-

G

-

A

-

S

-

G

-

S

-

R

-

E

-

V

-

D

G

V

T

I

I

H

L

A

A

D

G

T

K

I

V

V

A

V

L

A

A

G

G

G

A

Y

G

G

H

S

S

L

S

A

V

L

L

L

A

E

E

R

L

L

A

G

G

L

F

A

E

L

L

P

P

L

I

H

Q

P

S

L

H

R

P

V

L

H

Q

T

A

L

L

V

V

A

S

P

E

I

L

A

-

H

F

E

E

E

P

C

V

A

H

P

P

H

T

V

V

A

V

I

M

V

S

D

N

A

H

I

I

K

-

R
x
H

I

V

G
x
Y

I

V

S

S

R

Q

M

A

N

-

H

H

R

K

L

G

R

E

I

L

A

V

G

M

G

G

A

A

V

G

L

I

Q

D

G

S

A

Y

N

N

L

G

I

Y

D

G

K

Q

P

R

L

G

G

A

E

F

A

H

L

V

T

I

K

E

E

E

A

Q

L

M

A

A

L

-

G

-

Q

-

A

A

T

A

H

V

D

E

W

L

I

F

P

P

G

I

A

F

A

A

R

R

I

A

S

H

A

V

A

L

L

R

P

T

W

W

E

G

G

G

I

I

K

V

L

D

L

T

S

T

P

M

D

D

G

A

L

S

P

P

V

I

G

S

N

K

A

T

L

P

H

I

P

Q

R

N

L

L

F

Y

V

V

N

N

V

C

G

G

H

W

G
|
G

P

T

A

G

G
|
G

W

F

G
x
K

L

G

A

T

C

P

G

G

A

A

G

G

K

Y

L

T

I

L

A

A

D

H

L

T

V

I

A

-

G

-

Q

-

A

A

P

H

D

D

L

E

P

P

D

H

E

K

T

L

L

N

A

A

A

P

L

F

R

A

A

L

D

E

R

R

F

F

R

E

V197 (= V219) binding
H270 (≠ R304) mutation to A: 10-fold decrease in catalytic efficiency.
Y272 (≠ G306) mutation to A: 13000-fold decrease in catalytic efficiency.; mutation to F: 130-fold decrease in catalytic efficiency.
G354 (= G393) binding
G357 (= G396) binding
K359 (≠ G398) binding ; mutation K->A,D: Loss of activity.; mutation to R: Retains 0.07% of wild-type activity. Shows higher apparent KM for sarcosine.

Sites not aligning to the query:

31 binding
32 binding
53 binding
61 binding
62 binding
66 binding
68 binding
172 K→A: Retains 39% of wild-type activity.; K→D: Retains 32% of wild-type activity.; K→R: Retains 58% of wild-type activity.
173 modified: Tele-8alpha-FMN histidine

Query Sequence

>H281DRAFT_00908 FitnessBrowser__Burk376:H281DRAFT_00908
MDVIVIGGGIAGVATAYQLRAAGHRVCVVERHATVAQGATYGHGGTVLPTPLDVWFGPTF
MANRQNAKNGVISKPGFNGPARQFVKKLAELQEPDEFGRQYRLLRPLVELSREAMADMEA
RFGFEFEQASGLLHLVRSAQEWQQLQPALGLLALYEVPHHVLSPAECAAYEHSVRTEPEF
AGGVLFEHERTANCPLFTKLIKQTLDTQGGVQFMLGCEVSAIRLDGQRAAVELAPRPGAA
SGSREVDVIHADTIVVAGGYGSLALLERLGLALPLHPLRVHTLVAPIAHEECAPHVAIVD
AIKRIGISRMNHRLRIAGGAVLQGANLIDKPLGEALTKEALALLGQATHDWIPGAARISA
ALPWEGIKLLSPDGLPVVGNALHPRLFVNVGHGPAGWGLACGAGKLIADLVAGQAPDLPD
ETLAALRADRFR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory