SitesBLAST

Comparing H281DRAFT_01077 FitnessBrowser__Burk376:H281DRAFT_01077 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Found 20 (the maximum) hits to proteins with known functional sites

1krhA X-ray structure of benzoate dioxygenase reductase (see paper)
51% identity, 98% coverage: 2:334/340 of query aligns to 1:337/337 of 1krhA

• active site: C306 (= C303)
• binding flavin-adenine dinucleotide: Y143 (= Y141), R155 (= R153), S156 (= S154), Y157 (= Y155), S158 (= S156), V171 (≠ L169), V172 (≠ I170), G178 (= G176), K179 (≠ V177), M180 (= M178), S181 (= S179), T219 (= T216), E332 (= E329), F334 (= F331), S335 (≠ T332), A336 (≠ P333), N337 (= N334)
• binding fe2/s2 (inorganic) cluster: M38 (= M39), D39 (= D40), C40 (= C41), R41 (≠ S42), G43 (= G44), C45 (= C46), G46 (= G47), C48 (= C49), C82 (= C82)

5ogxA Crystal structure of amycolatopsis cytochrome p450 reductase gcob. (see paper)
32% identity, 94% coverage: 16:333/340 of query aligns to 9:331/333 of 5ogxA

• binding flavin-adenine dinucleotide: Y132 (= Y141), R144 (= R153), Q145 (≠ S154), Y146 (= Y155), S147 (= S156), H161 (vs. gap), V162 (≠ I170), V165 (≠ I173), G168 (= G176), V169 (= V177), A170 (≠ M178), T171 (≠ S179), T214 (= T216), F329 (= F331), P331 (= P333)
• binding fe2/s2 (inorganic) cluster: C34 (= C41), N35 (≠ S42), G37 (= G44), C39 (= C46), G40 (= G47), C42 (= C49), C74 (= C82)

Sites not aligning to the query:

• binding flavin-adenine dinucleotide: 333

P0DPQ8 Aromatic O-demethylase, reductase subunit; NADH--hemoprotein reductase; EC 1.6.2.- from Amycolatopsis sp. (strain ATCC 39116 / 75iv2) (see paper)
32% identity, 94% coverage: 16:333/340 of query aligns to 10:332/334 of P0DPQ8

• C35 (= C41) binding
• C40 (= C46) binding
• C43 (= C49) binding
• C75 (= C82) binding
• RQYS 145:148 (≠ RSYS 153:156) binding
• HVR 162:164 (≠ -IK 170:171) binding
• VAT 170:172 (≠ VMS 177:179) binding
• T215 (= T216) binding
• F330 (= F331) binding

Sites not aligning to the query:

• 334 binding

4wqmA Structure of the toluene 4-monooxygenase nadh oxidoreductase t4mof, k270s k271s variant (see paper)
29% identity, 97% coverage: 3:331/340 of query aligns to 1:325/326 of 4wqmA

• binding flavin-adenine dinucleotide: C36 (= C41), S38 (≠ D43), W57 (≠ G61), Y135 (= Y141), R146 (= R153), A147 (≠ S154), Y148 (= Y155), S149 (= S156), I162 (≠ L169), V163 (≠ I170), G169 (= G176), K170 (≠ V177), V171 (≠ M178), S172 (= S179), S209 (≠ T216), F325 (= F331)
• binding fe2/s2 (inorganic) cluster: Y34 (≠ M39), C36 (= C41), N37 (≠ S42), G39 (= G44), C41 (= C46), G42 (= G47), C44 (= C49), C76 (= C82)

Q03304 Toluene-4-monooxygenase system, ferredoxin--NAD(+) reductase component; T4MO; Ferredoxin--NAD(+) reductase; Toluene-4-monooxygenase systme, electron transfer component; EC 1.18.1.3 from Pseudomonas mendocina (see paper)
29% identity, 94% coverage: 12:331/340 of query aligns to 7:325/326 of Q03304

• C36 (= C41) binding
• C41 (= C46) binding
• C44 (= C49) binding
• C76 (= C82) binding
• RAYS 146:149 (≠ RSYS 153:156) binding
• IVK 162:164 (≠ LIK 169:171) binding
• KVS 170:172 (≠ VMS 177:179) binding

1tvcA Fad and nadh binding domain of methane monooxygenase reductase from methylococcus capsulatus (bath) (see paper)
31% identity, 59% coverage: 136:336/340 of query aligns to 44:249/250 of 1tvcA

• active site: Y63 (= Y155), S64 (= S156), L215 (= L302)
• binding dihydroflavine-adenine dinucleotide: F49 (≠ Y141), R61 (= R153), S62 (= S154), Y63 (= Y155), S64 (= S156), L78 (= L169), I79 (= I170), R80 (≠ K171), L82 (≠ I173), F87 (≠ M178), G128 (= G217), N155 (≠ T244), E159 (≠ D248), S186 (≠ R280), G187 (≠ K281), E242 (= E329), F244 (= F331), L245 (≠ T332)

Sites not aligning to the query:

• binding dihydroflavine-adenine dinucleotide: 24

7c3bC Ferredoxin reductase in carbazole 1,9a-dioxygenase (fad apo form) (see paper)
29% identity, 92% coverage: 20:331/340 of query aligns to 19:333/334 of 7c3bC

• binding fe2/s2 (inorganic) cluster: Y38 (≠ M39), C40 (= C41), A41 (≠ S42), G43 (= G44), C45 (= C46), G46 (= G47), C48 (= C49), C81 (= C82)

7c3aA Ferredoxin reductase in carbazole 1,9a-dioxygenase (see paper)
29% identity, 92% coverage: 20:331/340 of query aligns to 18:320/321 of 7c3aA

• binding flavin-adenine dinucleotide: C39 (= C41), S41 (≠ D43), W60 (≠ L69), R152 (= R153), A153 (≠ S154), Y154 (= Y155), S155 (= S156), Y169 (≠ L169), I170 (= I170), G176 (= G176), R177 (≠ V177), F178 (≠ M178), S179 (= S179), A218 (≠ T216), F320 (= F331)
• binding fe2/s2 (inorganic) cluster: Y37 (≠ M39), C39 (= C41), A40 (≠ S42), G42 (= G44), C44 (= C46), G45 (= G47), C47 (= C49), C80 (= C82)

Sites not aligning to the query:

• binding flavin-adenine dinucleotide: 321

7qu0A X-ray structure of fad domain of nqrf of klebsiella pneumoniae (see paper)
28% identity, 50% coverage: 153:322/340 of query aligns to 83:270/279 of 7qu0A

• binding ~{N}-[2,6-bis(fluoranyl)phenyl]ethanamide: S213 (≠ A272), F227 (≠ W283), M256 (= M308)
• binding flavin-adenine dinucleotide: R83 (= R153), A84 (≠ S154), Y85 (= Y155), S86 (= S156), A104 (≠ S174), T105 (vs. gap), P106 (vs. gap), P107 (vs. gap), A113 (vs. gap), P114 (vs. gap), P115 (≠ G175), G116 (= G176), I117 (≠ V177), M118 (= M178), S119 (= S179)

Sites not aligning to the query:

• binding flavin-adenine dinucleotide: 40, 279

7qtyA X-ray structure of fad domain of nqrf of klebsiella pneumoniae (see paper)
28% identity, 50% coverage: 153:322/340 of query aligns to 83:270/279 of 7qtyA

• binding 1-(furan-2-ylmethyl)-3-(2-methylphenyl)thiourea: A184 (≠ V243), R185 (≠ T244), S213 (≠ A272), F227 (≠ W283), H229 (≠ T285), V255 (≠ P307), M256 (= M308)
• binding flavin-adenine dinucleotide: R83 (= R153), A84 (≠ S154), Y85 (= Y155), S86 (= S156), N100 (≠ I170), A104 (≠ S174), T105 (vs. gap), P107 (vs. gap), A113 (vs. gap), P114 (vs. gap), G116 (= G176), I117 (≠ V177), M118 (= M178), S119 (= S179)

Sites not aligning to the query:

• binding flavin-adenine dinucleotide: 40, 279

8a1yF Sodium pumping nadh-quinone oxidoreductase with inhibitor hqno (see paper)
24% identity, 78% coverage: 67:331/340 of query aligns to 96:406/408 of 8a1yF

• binding flavin-adenine dinucleotide: R210 (= R153), A211 (≠ S154), Y212 (= Y155), S213 (= S156), V228 (≠ K171), R229 (≠ K172), A231 (≠ S174), V240 (vs. gap), P241 (vs. gap), G243 (= G176), Q244 (≠ V177), M245 (= M178)
• binding fe2/s2 (inorganic) cluster: C111 (= C82)

Sites not aligning to the query:

• binding fe2/s2 (inorganic) cluster: 70, 74, 76, 77, 79

A5F5Y4 Na(+)-translocating NADH-quinone reductase subunit F; Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F; EC 7.2.1.1 from Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) (see paper)
24% identity, 78% coverage: 67:331/340 of query aligns to 96:406/408 of A5F5Y4

• C111 (= C82) binding ; mutation to A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
• R210 (= R153) mutation to L: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.
• Y212 (= Y155) mutation to L: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.
• S246 (= S179) mutation to A: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.

Sites not aligning to the query:

• 70 binding ; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
• 76 binding ; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
• 79 binding ; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.

8a1uF Sodium pumping nadh-quinone oxidoreductase with substrates nadh and q2 (see paper)
24% identity, 78% coverage: 67:331/340 of query aligns to 95:405/405 of 8a1uF

• binding flavin-adenine dinucleotide: Y166 (= Y141), R209 (= R153), A210 (≠ S154), Y211 (= Y155), S212 (= S156), A230 (≠ S174), P233 (vs. gap), V239 (vs. gap), P240 (vs. gap), G242 (= G176), Q243 (≠ V177), M244 (= M178), A282 (≠ T216), F405 (= F331)
• binding fe2/s2 (inorganic) cluster: C110 (= C82)
• binding 1,4-dihydronicotinamide adenine dinucleotide: S212 (= S156), R228 (≠ K172), G281 (= G215), A282 (≠ T216), G309 (= G242), R311 (≠ T244), S339 (≠ E275), F353 (vs. gap), C377 (= C303), G378 (= G304), P379 (= P305), M381 (≠ P307), M382 (= M308), D403 (≠ E329)

Sites not aligning to the query:

• binding fe2/s2 (inorganic) cluster: 69, 71, 73, 75, 76, 78

2r6hC Crystal structure of the domain comprising the NAD binding and the fad binding regions of the nadh:ubiquinone oxidoreductase, na translocating, f subunit from porphyromonas gingivalis
26% identity, 53% coverage: 153:331/340 of query aligns to 86:288/289 of 2r6hC

• active site: C260 (= C303)
• binding flavin-adenine dinucleotide: R86 (= R153), A87 (≠ S154), Y88 (= Y155), S89 (= S156), N103 (≠ I170), A107 (≠ S174), T108 (≠ G175), I122 (vs. gap), K123 (vs. gap), P124 (vs. gap), G125 (= G176), I126 (≠ V177), S127 (≠ M178), S128 (= S179), F288 (= F331)

Sites not aligning to the query:

• binding flavin-adenine dinucleotide: 43

7c3bB Ferredoxin reductase in carbazole 1,9a-dioxygenase (fad apo form) (see paper)
29% identity, 92% coverage: 20:331/340 of query aligns to 18:305/306 of 7c3bB