SitesBLAST
Comparing H281DRAFT_01077 FitnessBrowser__Burk376:H281DRAFT_01077 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1krhA X-ray structure of benzoate dioxygenase reductase (see paper)
51% identity, 98% coverage: 2:334/340 of query aligns to 1:337/337 of 1krhA
- active site: C306 (= C303)
- binding flavin-adenine dinucleotide: Y143 (= Y141), R155 (= R153), S156 (= S154), Y157 (= Y155), S158 (= S156), V171 (≠ L169), V172 (≠ I170), G178 (= G176), K179 (≠ V177), M180 (= M178), S181 (= S179), T219 (= T216), E332 (= E329), F334 (= F331), S335 (≠ T332), A336 (≠ P333), N337 (= N334)
- binding fe2/s2 (inorganic) cluster: M38 (= M39), D39 (= D40), C40 (= C41), R41 (≠ S42), G43 (= G44), C45 (= C46), G46 (= G47), C48 (= C49), C82 (= C82)
5ogxA Crystal structure of amycolatopsis cytochrome p450 reductase gcob. (see paper)
32% identity, 94% coverage: 16:333/340 of query aligns to 9:331/333 of 5ogxA
- binding flavin-adenine dinucleotide: Y132 (= Y141), R144 (= R153), Q145 (≠ S154), Y146 (= Y155), S147 (= S156), H161 (vs. gap), V162 (≠ I170), V165 (≠ I173), G168 (= G176), V169 (= V177), A170 (≠ M178), T171 (≠ S179), T214 (= T216), F329 (= F331), P331 (= P333)
- binding fe2/s2 (inorganic) cluster: C34 (= C41), N35 (≠ S42), G37 (= G44), C39 (= C46), G40 (= G47), C42 (= C49), C74 (= C82)
Sites not aligning to the query:
P0DPQ8 Aromatic O-demethylase, reductase subunit; NADH--hemoprotein reductase; EC 1.6.2.- from Amycolatopsis sp. (strain ATCC 39116 / 75iv2) (see paper)
32% identity, 94% coverage: 16:333/340 of query aligns to 10:332/334 of P0DPQ8
Sites not aligning to the query:
4wqmA Structure of the toluene 4-monooxygenase nadh oxidoreductase t4mof, k270s k271s variant (see paper)
29% identity, 97% coverage: 3:331/340 of query aligns to 1:325/326 of 4wqmA
- binding flavin-adenine dinucleotide: C36 (= C41), S38 (≠ D43), W57 (≠ G61), Y135 (= Y141), R146 (= R153), A147 (≠ S154), Y148 (= Y155), S149 (= S156), I162 (≠ L169), V163 (≠ I170), G169 (= G176), K170 (≠ V177), V171 (≠ M178), S172 (= S179), S209 (≠ T216), F325 (= F331)
- binding fe2/s2 (inorganic) cluster: Y34 (≠ M39), C36 (= C41), N37 (≠ S42), G39 (= G44), C41 (= C46), G42 (= G47), C44 (= C49), C76 (= C82)
Q03304 Toluene-4-monooxygenase system, ferredoxin--NAD(+) reductase component; T4MO; Ferredoxin--NAD(+) reductase; Toluene-4-monooxygenase systme, electron transfer component; EC 1.18.1.3 from Pseudomonas mendocina (see paper)
29% identity, 94% coverage: 12:331/340 of query aligns to 7:325/326 of Q03304
1tvcA Fad and nadh binding domain of methane monooxygenase reductase from methylococcus capsulatus (bath) (see paper)
31% identity, 59% coverage: 136:336/340 of query aligns to 44:249/250 of 1tvcA
- active site: Y63 (= Y155), S64 (= S156), L215 (= L302)
- binding dihydroflavine-adenine dinucleotide: F49 (≠ Y141), R61 (= R153), S62 (= S154), Y63 (= Y155), S64 (= S156), L78 (= L169), I79 (= I170), R80 (≠ K171), L82 (≠ I173), F87 (≠ M178), G128 (= G217), N155 (≠ T244), E159 (≠ D248), S186 (≠ R280), G187 (≠ K281), E242 (= E329), F244 (= F331), L245 (≠ T332)
Sites not aligning to the query:
7c3bC Ferredoxin reductase in carbazole 1,9a-dioxygenase (fad apo form) (see paper)
29% identity, 92% coverage: 20:331/340 of query aligns to 19:333/334 of 7c3bC
7c3aA Ferredoxin reductase in carbazole 1,9a-dioxygenase (see paper)
29% identity, 92% coverage: 20:331/340 of query aligns to 18:320/321 of 7c3aA
- binding flavin-adenine dinucleotide: C39 (= C41), S41 (≠ D43), W60 (≠ L69), R152 (= R153), A153 (≠ S154), Y154 (= Y155), S155 (= S156), Y169 (≠ L169), I170 (= I170), G176 (= G176), R177 (≠ V177), F178 (≠ M178), S179 (= S179), A218 (≠ T216), F320 (= F331)
- binding fe2/s2 (inorganic) cluster: Y37 (≠ M39), C39 (= C41), A40 (≠ S42), G42 (= G44), C44 (= C46), G45 (= G47), C47 (= C49), C80 (= C82)
Sites not aligning to the query:
7qu0A X-ray structure of fad domain of nqrf of klebsiella pneumoniae (see paper)
28% identity, 50% coverage: 153:322/340 of query aligns to 83:270/279 of 7qu0A
- binding ~{N}-[2,6-bis(fluoranyl)phenyl]ethanamide: S213 (≠ A272), F227 (≠ W283), M256 (= M308)
- binding flavin-adenine dinucleotide: R83 (= R153), A84 (≠ S154), Y85 (= Y155), S86 (= S156), A104 (≠ S174), T105 (vs. gap), P106 (vs. gap), P107 (vs. gap), A113 (vs. gap), P114 (vs. gap), P115 (≠ G175), G116 (= G176), I117 (≠ V177), M118 (= M178), S119 (= S179)
Sites not aligning to the query:
7qtyA X-ray structure of fad domain of nqrf of klebsiella pneumoniae (see paper)
28% identity, 50% coverage: 153:322/340 of query aligns to 83:270/279 of 7qtyA
- binding 1-(furan-2-ylmethyl)-3-(2-methylphenyl)thiourea: A184 (≠ V243), R185 (≠ T244), S213 (≠ A272), F227 (≠ W283), H229 (≠ T285), V255 (≠ P307), M256 (= M308)
- binding flavin-adenine dinucleotide: R83 (= R153), A84 (≠ S154), Y85 (= Y155), S86 (= S156), N100 (≠ I170), A104 (≠ S174), T105 (vs. gap), P107 (vs. gap), A113 (vs. gap), P114 (vs. gap), G116 (= G176), I117 (≠ V177), M118 (= M178), S119 (= S179)
Sites not aligning to the query:
8a1yF Sodium pumping nadh-quinone oxidoreductase with inhibitor hqno (see paper)
24% identity, 78% coverage: 67:331/340 of query aligns to 96:406/408 of 8a1yF
- binding flavin-adenine dinucleotide: R210 (= R153), A211 (≠ S154), Y212 (= Y155), S213 (= S156), V228 (≠ K171), R229 (≠ K172), A231 (≠ S174), V240 (vs. gap), P241 (vs. gap), G243 (= G176), Q244 (≠ V177), M245 (= M178)
- binding fe2/s2 (inorganic) cluster: C111 (= C82)
Sites not aligning to the query:
A5F5Y4 Na(+)-translocating NADH-quinone reductase subunit F; Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F; EC 7.2.1.1 from Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) (see paper)
24% identity, 78% coverage: 67:331/340 of query aligns to 96:406/408 of A5F5Y4
- C111 (= C82) binding ; mutation to A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
- R210 (= R153) mutation to L: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.
- Y212 (= Y155) mutation to L: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.
- S246 (= S179) mutation to A: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.
Sites not aligning to the query:
- 70 binding ; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
- 76 binding ; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
- 79 binding ; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
8a1uF Sodium pumping nadh-quinone oxidoreductase with substrates nadh and q2 (see paper)
24% identity, 78% coverage: 67:331/340 of query aligns to 95:405/405 of 8a1uF
- binding flavin-adenine dinucleotide: Y166 (= Y141), R209 (= R153), A210 (≠ S154), Y211 (= Y155), S212 (= S156), A230 (≠ S174), P233 (vs. gap), V239 (vs. gap), P240 (vs. gap), G242 (= G176), Q243 (≠ V177), M244 (= M178), A282 (≠ T216), F405 (= F331)
- binding fe2/s2 (inorganic) cluster: C110 (= C82)
- binding 1,4-dihydronicotinamide adenine dinucleotide: S212 (= S156), R228 (≠ K172), G281 (= G215), A282 (≠ T216), G309 (= G242), R311 (≠ T244), S339 (≠ E275), F353 (vs. gap), C377 (= C303), G378 (= G304), P379 (= P305), M381 (≠ P307), M382 (= M308), D403 (≠ E329)
Sites not aligning to the query:
2r6hC Crystal structure of the domain comprising the NAD binding and the fad binding regions of the nadh:ubiquinone oxidoreductase, na translocating, f subunit from porphyromonas gingivalis
26% identity, 53% coverage: 153:331/340 of query aligns to 86:288/289 of 2r6hC
- active site: C260 (= C303)
- binding flavin-adenine dinucleotide: R86 (= R153), A87 (≠ S154), Y88 (= Y155), S89 (= S156), N103 (≠ I170), A107 (≠ S174), T108 (≠ G175), I122 (vs. gap), K123 (vs. gap), P124 (vs. gap), G125 (= G176), I126 (≠ V177), S127 (≠ M178), S128 (= S179), F288 (= F331)
Sites not aligning to the query:
7c3bB Ferredoxin reductase in carbazole 1,9a-dioxygenase (fad apo form) (see paper)
29% identity, 92% coverage: 20:331/340 of query aligns to 18:305/306 of 7c3bB
7romA Crystal structure of saccharomyces cerevisiae nadh-cytochrome b5 reductase 1 (cbr1) fragment (residues 28-284) bound to fad
29% identity, 55% coverage: 128:314/340 of query aligns to 38:232/255 of 7romA
- binding flavin-adenine dinucleotide: R61 (= R153), S62 (= S154), Y63 (= Y155), T64 (≠ S156), L79 (= L169), V80 (≠ I170), Y83 (≠ I173), T85 (≠ G175), G86 (= G176), N87 (≠ V177), V88 (≠ M178), S89 (= S179), T127 (= T216), A130 (= A219)
7qu3A X-ray structure of fad domain of nqrf of pseudomonas aeruginosa (see paper)
27% identity, 50% coverage: 153:322/340 of query aligns to 83:269/279 of 7qu3A
- binding flavin-adenine dinucleotide: R83 (= R153), A84 (≠ S154), Y85 (= Y155), S86 (= S156), N100 (≠ I170), A104 (≠ S174), S105 (≠ G175), L112 (vs. gap), P113 (vs. gap), G115 (= G176), Q116 (≠ V177), M117 (= M178), S118 (= S179), A155 (≠ T216)
- binding 4-(benzimidazol-1-ylmethyl)benzenecarbonitrile: R102 (≠ K172), G154 (= G215), G182 (= G242), F226 (vs. gap), I227 (≠ V284), P252 (= P305), M255 (= M308)
Sites not aligning to the query:
7qu5A X-ray structure of fad domain of nqrf of pseudomonas aeruginosa (see paper)
27% identity, 50% coverage: 153:322/340 of query aligns to 83:269/280 of 7qu5A
- binding flavin-adenine dinucleotide: R83 (= R153), A84 (≠ S154), Y85 (= Y155), S86 (= S156), N100 (≠ I170), A104 (≠ S174), L112 (vs. gap), P113 (vs. gap), G115 (= G176), Q116 (≠ V177), M117 (= M178), S118 (= S179), A155 (≠ T216)
- binding gamma-Valerolactone: S105 (≠ G175), P108 (vs. gap), D196 (≠ Q256), W207 (≠ F267), H208 (≠ S268), L209 (≠ T269)
Sites not aligning to the query:
4u9uA Crystal structure of nqrf fad-binding domain from vibrio cholerae (see paper)
27% identity, 50% coverage: 153:322/340 of query aligns to 81:268/279 of 4u9uA
- binding flavin-adenine dinucleotide: R81 (= R153), A82 (≠ S154), Y83 (= Y155), S84 (= S156), N98 (≠ I170), A102 (≠ S174), T103 (vs. gap), P104 (vs. gap), V111 (vs. gap), P112 (vs. gap), G114 (= G176), Q115 (≠ V177), M116 (= M178), S117 (= S179)
- binding magnesium ion: R186 (≠ L247), M188 (≠ L249)
- binding sodium ion: A198 (≠ T259), N201 (≠ L262), D202 (≠ P263), F204 (= F265)
Sites not aligning to the query:
3ozwA The crystal structure of flavohemoglobin from r. Eutrophus in complex with ketoconazole (see paper)
29% identity, 60% coverage: 130:334/340 of query aligns to 178:399/403 of 3ozwA
- binding flavin-adenine dinucleotide: R206 (= R153), Q207 (≠ S154), Y208 (= Y155), S209 (= S156), S222 (≠ L169), V223 (≠ I170), K224 (= K171), E226 (≠ I173), P232 (vs. gap), G234 (vs. gap), Y235 (vs. gap), V236 (= V177), S237 (≠ M178), V276 (≠ T216), T279 (≠ A219), V395 (≠ K330), F396 (= F331)
- binding 1-acetyl-4-(4-{[(2R,4S)-2-(2,4-dichlorophenyl)-2-(1H-imidazol-1-ylmethyl)-1,3-dioxolan-4-yl]methoxy}phenyl)piperazine: P398 (= P333)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 46, 50
- binding protoporphyrin ix containing fe: 42, 43, 81, 85, 88, 90, 94, 95, 98, 126, 130, 133
- binding 1-acetyl-4-(4-{[(2R,4S)-2-(2,4-dichlorophenyl)-2-(1H-imidazol-1-ylmethyl)-1,3-dioxolan-4-yl]methoxy}phenyl)piperazine: 53, 56, 102
Query Sequence
>H281DRAFT_01077 FitnessBrowser__Burk376:H281DRAFT_01077
MSSYNIALNFEDGVTRFVECKAGEKVLDAAFRARINLPMDCSDGVCGTCKCRAESGRYDL
GDDYIEDALTDDEKQSGLVLTCQMVPESDCVIAVPASSTACKTEQSKFAATVTKVEKHND
AAVVLEMDVDGTPPVFLAGQYVNIDVPGSGQHRSYSFSSLPGESKICFLIKKISGGVMST
WLESAQPGNKVQLTGPMGTFYLRAVERPLLFLAGGTGLAPFLSMLEVLARANSQQKVHLI
YGVTRDLDLVLVEDIQAYTLRLPNFTFSTVVAATESVHPRKGWVTQHMPAECINDGDVDV
YLCGPPPMVDAVRQYFDENGVEPASFHYEKFTPNAALVDA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory