SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_01156 FitnessBrowser__Burk376:H281DRAFT_01156 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

8gxkB Pseudomonas jinjuensis n-acetyltransferase (see paper)
44% identity, 70% coverage: 53:187/192 of query aligns to 52:184/188 of 8gxkB

query
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8gxkB

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binding coenzyme a: T93 (= T96), W94 (= W97), H100 (≠ Q103), G101 (≠ R104), T129 (= T132), N133 (= N136), L134 (≠ D137), R135 (≠ K138), S136 (= S139), A139 (= A142), K142 (≠ R145)

Sites not aligning to the query:

binding coenzyme a: 40

1yreB Hypothetical protein pa3270 from pseudomonas aeruginosa in complex with coa
37% identity, 80% coverage: 37:189/192 of query aligns to 37:186/187 of 1yreB

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binding coenzyme a: M40 (≠ V40), T93 (= T96), W94 (= W97), H100 (≠ Q103), G101 (≠ R104), N105 (= N108), R135 (≠ K138), G138 (≠ A141)

8gxfB Pseudomonas flexibilis gcn5 family acetyltransferase (see paper)
35% identity, 93% coverage: 12:189/192 of query aligns to 15:186/187 of 8gxfB

query
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binding coenzyme a: T93 (= T96), H100 (≠ Q103), G101 (≠ R104), K109 (= K112), T129 (= T132), R135 (≠ K138)

6c37A Mycobacterium smegmatis rimj in complex with coa-disulfide
28% identity, 80% coverage: 30:183/192 of query aligns to 51:197/209 of 6c37A

query
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binding [[(2~{S},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{R})-4-[[3-[2-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyldisulfanyl]ethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate: G110 (= G94), Y111 (≠ H95), W112 (= W97), V113 (≠ L98), T118 (≠ Q103), G119 (≠ R104), G121 (≠ A106), A123 (≠ N108), T124 (= T109), T146 (= T131), N151 (= N136), T152 (≠ D137), P153 (≠ K138), S154 (= S139), V157 (≠ A142), H160 (≠ R145)

Sites not aligning to the query:

binding [[(2~{S},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{R})-4-[[3-[2-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyldisulfanyl]ethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate: 42, 205

6c32A Mycobacterium smegmatis rimj with accoa
28% identity, 80% coverage: 30:183/192 of query aligns to 51:197/209 of 6c32A

query
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binding acetyl coenzyme *a: G110 (= G94), Y111 (≠ H95), W112 (= W97), V113 (≠ L98), T118 (≠ Q103), G119 (≠ R104), G121 (≠ A106), A123 (≠ N108), T124 (= T109), T146 (= T131), N151 (= N136), T152 (≠ D137), P153 (≠ K138), S154 (= S139), V157 (≠ A142), H160 (≠ R145)

Sites not aligning to the query:

binding acetyl coenzyme *a: 205

Query Sequence

>H281DRAFT_01156 FitnessBrowser__Burk376:H281DRAFT_01156
MQLTLIGKTVELRPLERDHAQALLDAAADGQLWNLKLTVVPGPDTIGGYIDTALAGRDAG
TVMPFVIVRRETGTVIGSTRFWKIDRANRKLEIGHTWLCASAQRSAANTEAKYLLLRHAF
EAMQCVRVQFTTDELNDKSRAAILRIGAKQEGVVRHERIMPDGRKRNSVRFSIIDDEWAQ
VKAMLETKLGPR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory