SitesBLAST
Comparing H281DRAFT_01168 FitnessBrowser__Burk376:H281DRAFT_01168 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 14 hits to proteins with known functional sites (download)
1efpB Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
68% identity, 100% coverage: 1:216/217 of query aligns to 1:220/246 of 1efpB
- binding adenosine monophosphate: P6 (≠ A6), V7 (= V7), D39 (= D39), I63 (≠ A63), V101 (= V97), A119 (≠ L115), G120 (= G116), Q122 (= Q118), A123 (= A119), N126 (≠ D122), M128 (≠ S124), N129 (= N125), T131 (= T127)
- binding flavin-adenine dinucleotide: Y13 (= Y13), L182 (= L178)
P38117 Electron transfer flavoprotein subunit beta; Beta-ETF from Homo sapiens (Human) (see 10 papers)
64% identity, 99% coverage: 1:215/217 of query aligns to 4:222/255 of P38117
- A9 (= A6) binding
- NPFC 39:42 (≠ NPFD 36:39) binding
- C66 (≠ A63) binding
- 123:134 (vs. 116:127, 92% identical) binding
- D128 (= D121) to N: in GA2B; decreased protein stability; dbSNP:rs104894678
- T154 (≠ V147) to M: in dbSNP:rs1130426
- R164 (= R157) to Q: in GA2B; reduced electron transfer activity; dbSNP:rs104894677
- E165 (= E158) mutation E->A,Q: Drastically increases interprotein electron transfer rates.
- 183:205 (vs. 176:198, 87% identical) Recognition loop
- L195 (= L188) mutation to A: Severely impaired in complex formation with ACADM.
- K200 (= K193) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- KAK 200:202 (= KAK 193:195) mutation to RAR: Does not abolish methylation by ETFBKMT.
- KAKK 200:203 (= KAKK 193:196) mutation to RAKR: Does not abolish electron transfer activity. Abolishes sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- K203 (= K196) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
2a1uB Crystal structure of the human etf e165betaa mutant (see paper)
63% identity, 99% coverage: 1:215/217 of query aligns to 1:219/252 of 2a1uB
- binding adenosine monophosphate: A6 (= A6), V7 (= V7), K8 (= K8), C39 (≠ D39), C63 (≠ A63), V101 (= V97), L119 (= L115), G120 (= G116), Q122 (= Q118), A123 (= A119), D126 (= D122), C128 (≠ S124), N129 (= N125), Q130 (= Q126), T131 (= T127)
- binding flavin-adenine dinucleotide: P37 (= P37), L182 (= L178)
Q2TBV3 Electron transfer flavoprotein subunit beta; Beta-ETF from Bos taurus (Bovine) (see paper)
62% identity, 99% coverage: 1:215/217 of query aligns to 4:222/255 of Q2TBV3
- K200 (= K193) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (= K196) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
Q9DCW4 Electron transfer flavoprotein subunit beta; Beta-ETF from Mus musculus (Mouse) (see paper)
61% identity, 99% coverage: 1:215/217 of query aligns to 4:222/255 of Q9DCW4
- K200 (= K193) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (= K196) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
P53570 Electron transfer flavoprotein subunit beta; Beta-ETF; Electron transfer flavoprotein small subunit; ETFSS from Methylophilus methylotrophus (Bacterium W3A1) (see paper)
34% identity, 96% coverage: 1:209/217 of query aligns to 1:215/264 of P53570
6fahB Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
36% identity, 96% coverage: 1:209/217 of query aligns to 1:211/262 of 6fahB
- binding flavin-adenine dinucleotide: C6 (≠ A6), A7 (≠ V7), K8 (= K8), D38 (= D39), T61 (≠ S62), M62 (≠ A63), A93 (≠ L92), D94 (≠ Q93), T95 (≠ P94), T98 (≠ V97), L102 (= L101), G118 (= G116), Q120 (= Q118), G124 (≠ D122), T126 (≠ S124), A127 (≠ N125), V129 (≠ T127)
Sites not aligning to the query:
1o96A Structure of electron transferring flavoprotein for methylophilus methylotrophus. (see paper)
34% identity, 96% coverage: 1:209/217 of query aligns to 1:214/261 of 1o96A
- binding adenosine monophosphate: A6 (= A6), V7 (= V7), K8 (= K8), D38 (= D39), V63 (≠ A63), V99 (= V97), A117 (≠ L115), G118 (= G116), Q120 (= Q118), S121 (≠ A119), Y126 (≠ S124), A127 (≠ N125), T129 (= T127)
- binding flavin-adenine dinucleotide: Q120 (= Q118), Q182 (≠ D177)
5ol2B The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
32% identity, 97% coverage: 1:210/217 of query aligns to 1:211/260 of 5ol2B
- binding flavin-adenine dinucleotide: C6 (≠ A6), I7 (≠ V7), K8 (= K8), D38 (= D39), M61 (≠ A63), A92 (≠ L92), D93 (≠ Q93), T94 (≠ P94), T97 (≠ V97), A115 (≠ L115), G116 (= G116), A119 (= A119), G122 (≠ D122), T124 (≠ S124), A125 (≠ N125), V127 (≠ T127)
4l2iB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
32% identity, 90% coverage: 1:196/217 of query aligns to 1:196/263 of 4l2iB
- binding flavin-adenine dinucleotide: C6 (≠ A6), V7 (= V7), K8 (= K8), Y37 (≠ F38), D38 (= D39), Y40 (≠ I41), T60 (≠ S62), M61 (≠ A63), A92 (≠ S89), D93 (≠ E90), T94 (≠ E91), T97 (≠ Q93), M101 (≠ V97), C116 (≠ L115), G117 (= G116), Q119 (= Q118), A120 (= A119), G123 (≠ D122), T125 (≠ S124), A126 (≠ N125), V128 (≠ T127)
- binding nicotinamide-adenine-dinucleotide: M61 (≠ A63), G62 (= G64), P63 (≠ V65), S85 (≠ E87), D86 (vs. gap), G91 (≠ S88)
4kpuB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
32% identity, 90% coverage: 1:196/217 of query aligns to 1:196/263 of 4kpuB
- binding flavin-adenine dinucleotide: C6 (≠ A6), V7 (= V7), K8 (= K8), Y37 (≠ F38), D38 (= D39), Y40 (≠ I41), M61 (≠ A63), A92 (≠ S89), D93 (≠ E90), T94 (≠ E91), T97 (≠ Q93), C116 (≠ L115), G117 (= G116), Q119 (= Q118), A120 (= A119), G123 (≠ D122), T125 (≠ S124), A126 (≠ N125), V128 (≠ T127)
7koeA Electron bifurcating flavoprotein fix/etfabcx (see paper)
31% identity, 96% coverage: 3:210/217 of query aligns to 4:219/282 of 7koeA
- binding flavin-adenine dinucleotide: C7 (≠ A6), I8 (≠ V7), K9 (= K8), D39 (= D39), M62 (≠ A63), A93 (≠ L92), D94 (≠ Q93), T95 (≠ P94), T98 (≠ V97), L102 (= L101), T121 (≠ L115), G122 (= G116), A125 (= A119), G128 (≠ D122), T130 (≠ S124), G131 (≠ N125), V133 (≠ T127)
5ow0B Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
32% identity, 94% coverage: 1:205/217 of query aligns to 1:204/251 of 5ow0B
- binding adenosine monophosphate: L6 (≠ A6), A7 (≠ V7), K8 (= K8), D39 (= D39), Y62 (≠ A63), I95 (≠ V97), A114 (≠ L115), G115 (= G116), Q117 (= Q118), S118 (≠ A119), R123 (≠ S124), G124 (≠ N125), V125 (≠ Q126), V126 (≠ T127), P127 (≠ G128)
- binding flavin-adenine dinucleotide: L37 (vs. gap), Y38 (≠ F38)
7qh2B Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
30% identity, 91% coverage: 2:198/217 of query aligns to 3:201/265 of 7qh2B
- binding flavin-adenine dinucleotide: C7 (≠ A6), K9 (= K8), D39 (= D39), M62 (≠ A63), D94 (vs. gap), V95 (vs. gap), L102 (≠ V97), G118 (= G116), Q120 (= Q118), T121 (≠ A119), T126 (≠ S124), A127 (≠ N125), V129 (≠ T127)
Query Sequence
>H281DRAFT_01168 FitnessBrowser__Burk376:H281DRAFT_01168
MKILVAVKRVVDYNVKVRVKSDGTGVDIANVKMSMNPFDEIAVEEAVRLKEAGVATEVIA
VSAGVTQSQETLRTALAIGADRAILIESSEELQPLAVAKLLKALVDKEQPQLVILGKQAI
DDDSNQTGQMLAALANLPQATFASKVVVADGKATVSREVDGGAETLSLTLPAVVTTDLRL
NEPRYVTLPNIMKAKKKPLETIRPEDLGVDVAPRLKT
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory