SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_01297 FitnessBrowser__Burk376:H281DRAFT_01297 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

Q02I31 Putative pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Pterin carbinolamine dehydratase; PCD; EC 4.2.1.96 from Pseudomonas aeruginosa (strain UCBPP-PA14) (see paper)
34% identity, 64% coverage: 9:94/135 of query aligns to 20:108/118 of Q02I31

query
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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: Phosphothreonine

2v6tB Crystal structure of a complex of pterin-4a-carbinolamine dehydratase from toxoplasma gondii with 7,8-dihydrobiopterin (see paper)
36% identity, 53% coverage: 24:94/135 of query aligns to 21:93/100 of 2v6tB

query
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2v6tB

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active site: D54 (≠ E55), H58 (= H59), H59 (= H60), H76 (= H77), D77 (≠ S78), D85 (= D86)
binding 2-amino-6-(1,2-dihydroxy-propyl)-7,8-dihydro-6h-pteridin-4-one: D57 (≠ W58), H58 (= H59), H59 (= H60), D77 (≠ S78)

Query Sequence

>H281DRAFT_01297 FitnessBrowser__Burk376:H281DRAFT_01297
MAKTEKTYTDEEIQQRLVGPLQHWYVEDGWLRRKYRTEGWKGTLMVVNAVGHLAEAAWHH
PDLTVSYAFVIVKLKTHSAKGITDKDFALANKIESFIQWQPATEGGPLEGTPSDDQRFRY
IKYDAAKAEGAAEGG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory