SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_01585 H281DRAFT_01585 amino acid/amide ABC transporter ATP-binding protein 2, HAAT family to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1ji0A Crystal structure analysis of the abc transporter from thermotoga maritima
46% identity, 100% coverage: 1:237/237 of query aligns to 2:239/240 of 1ji0A

query
sites
1ji0A

M

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x
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A

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binding adenosine-5'-triphosphate: Y16 (= Y15), G42 (= G41), G44 (= G43), K45 (= K44), T46 (≠ S45), T47 (= T46), E88 (= E87), E165 (= E163)

6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
31% identity, 97% coverage: 5:235/237 of query aligns to 2:234/240 of 6mjpA

query
sites
6mjpA

M

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binding calcium ion: E111 (≠ Q114), D115 (≠ E118)

6mhzA Vanadate trapped cryo-em structure of e.Coli lptb2fg transporter (see paper)
34% identity, 94% coverage: 13:235/237 of query aligns to 11:234/235 of 6mhzA

query
sites
6mhzA

A

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binding adp orthovanadate: Y12 (≠ F14), N37 (= N40), G38 (= G41), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46), Q84 (≠ E87), S136 (≠ Q137), S138 (= S139), G139 (= G140), G140 (= G141), E162 (= E163), G166 (= G167), H194 (≠ Q195)

6b89A E. Coli lptb in complex with adp and novobiocin (see paper)
34% identity, 94% coverage: 13:235/237 of query aligns to 11:234/234 of 6b89A

query
sites
6b89A

A

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binding adenosine-5'-diphosphate: Y12 (≠ F14), R15 (= R17), V17 (≠ I20), N37 (= N40), G38 (= G41), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46)
binding magnesium ion: T42 (≠ S45), Q84 (≠ E87)
binding novobiocin: L71 (≠ P74), H72 (≠ Y75), P83 (= P86), A86 (≠ R89), S87 (≠ R90), F89 (= F92), R90 (≠ A93), R91 (≠ D94), L92 (= L95), V101 (≠ G104), Q135 (≠ G136), R149 (= R150)

4p31A Crystal structure of a selenomethionine derivative of e. Coli lptb in complex with adp-magensium (see paper)
34% identity, 94% coverage: 13:235/237 of query aligns to 11:234/234 of 4p31A

query
sites
4p31A

A

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L

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N

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G

A

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x
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binding adenosine-5'-diphosphate: Y12 (≠ F14), R15 (= R17), V17 (≠ I20), G38 (= G41), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46)
binding magnesium ion: T42 (≠ S45), Q84 (≠ E87)

6s8nB Cryo-em structure of lptb2fgc in complex with lipopolysaccharide (see paper)
34% identity, 94% coverage: 13:235/237 of query aligns to 11:234/238 of 6s8nB

query
sites
6s8nB

A

A

F

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L

I

L

V

P

P

R

R

R

D

Q

A

G

G

E

N

V

I

I

I

F

I

R

D

G

D

Q

D

N

D

I

I

A

S

A

L

L

L

A

P

P

L

Y

H

K

A

I

R

A

A

R

R

M

R

G

G

M

I

G

G

F

Y

V

L

P

P

E

Q

D

E

R

A

R

S

V

I

F

F

A
x
R

D

R

L

L

T

S

V

V

M

Y

E

D

N

N

L

L

D

M

T

A

G

V

R

L

Q

Q

P

I

P

R

R

D

E

D

G

L

A

S

P

A

Q

E

W

Q

T

R

P

E

E

D

K

R

L

A

F

N

R

E

L

L

F

M

P

E

-

E

-

F

N

H

L

I

G

E

E

H

M

L

P

R

K

D

R

S

P
x
M

G
|
G

G
x
Q

Q

S

M

L

S

S

G

G

E

E

Q

R

M

R

L

V

T

E

V

I

S

A

R

R

T

A

L

L

M

A

G

A

N

N

P

P

Y

K

L

F

V

I

L

L

D

D

E

E

P

P

S

F

E

A

G

G

V

V

A

D

P

P

V

I

I

S

V

V

E

I

Q

D

M

I

A

K

N

R

M

I

I

I

L

E

E

H

L

L

K

R

R

D

E

S

G

G

L

L

S

G

I

V

L

L

L

I

S

T

E

D

Q

H

N

N

L

V

H

R

F

E

A

T

E

L

L

A

V

V

S

C

D

E

R

R

A

A

Y

Y

V

I

L

V

E

S

K

Q

G

G

Q

H

I

L

R

I

F

A

S

H

G

G

T

T

I

P

G

T

E

E

L

I

A

L

S

Q

N

D

E

E

T

H

V

V

R

K

R

R

A

V

Y

Y

L

L

binding 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione: R90 (≠ A93), M133 (≠ P134), G134 (= G135), Q135 (≠ G136)

6s8gA Cryo-em structure of lptb2fgc in complex with amp-pnp (see paper)
34% identity, 94% coverage: 13:235/237 of query aligns to 11:234/238 of 6s8gA

query
sites
6s8gA

A

A

F
x
Y

Y

K

G

G

R
|
R

A

-

H

R

I

V

L

V

F

E

D

D

V

V

G

S

L

L

E

T

V

V

G

N

R

S

G

G

E

E

V

I

V

V

A

G

L

L

M

L

G

G

R

P

N
|
N

G

G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

T

T

M

F

K

Y

A

M

V

V

M

V

G

G

L

I

L

V

P

P

R

R

R

D

Q

A

G

G

E

N

V

I

I

I

F

I

R

D

G

D

Q

D

N

D

I

I

A

S

A

L

L

L

A

P

P

L

Y

H

K

A

I

R

A

A

R

R

M

R

G

G

M

I

G

G

F

Y

V

L

P

P

E
x
Q

D

E

R

A

R

S

V

I

F

F

A

R

D

R

L

L

T

S

V

V

M

Y

E

D

N

N

L

L

D

M

T

A

G

V

R

L

Q

Q

P

I

P

R

R

D

E

D

G

L

A

S

P

A

Q

E

W

Q

T

R

P

E

E

D

K

R

L

A

F

N

R

E

L

L

F

M

P

E

-

E

-

F

N

H

L

I

G

E

E

H

M

L

P

R

K

D

R

S

P

M

G

G

G

Q

Q
x
S

M

L

S
|
S

G

G

E
|
E

Q

R

M

R

L

V

T

E

V

I

S

A

R

R

T

A

L

L

M

A

G

A

N

N

P

P

Y

K

L

F

V

I

L

L

D

D

E

E

P

P

S

F

E

A

G

G

V

V

A

D

P

P

V

I

I

S

V

V

E

I

Q

D

M

I

A

K

N

R

M

I

I

I

L

E

E

H

L

L

K

R

R

D

E

S

G

G

L

L

S

G

I

V

L

L

L

I

S

T

E

D

Q

H

N

N

L

V

H

R

F

E

A

T

E

L

L

A

V

V

S

C

D

E

R

R

A

A

Y

Y

V

I

L

V

E

S

K

Q

G

G

Q

H

I

L

R

I

F

A

S

H

G

G

T

T

I

P

G

T

E

E

L

I

A

L

S

Q

N

D

E

E

T

H

V

V

R

K

R

R

A

V

Y

Y

L

L

binding phosphoaminophosphonic acid-adenylate ester: Y12 (≠ F14), R15 (= R17), N37 (= N40), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46), Q84 (≠ E87), S136 (≠ Q137), S138 (= S139), E141 (= E142)

6mbnA Lptb e163q in complex with atp (see paper)
33% identity, 94% coverage: 13:235/237 of query aligns to 12:235/241 of 6mbnA

query
sites
6mbnA

A

A

F
x
Y

Y

K

G

G

R
|
R

A

-

H

R

I
x
V

L

V

F

E

D

D

V

V

G

S

L

L

E

T

V

V

G

N

R

S

G

G

E

E

V

I

V

V

A

G

L

L

M

L

G

G

R

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

T

T

M

F

K

Y

A

M

V

V

M

V

G

G

L

I

L

V

P

P

R

R

R

D

Q

A

G

G

E

N

V

I

I

I

F

I

R

D

G

D

Q

D

N

D

I

I

A

S

A

L

L

L

A

P

P

L

Y

H

K

A

I

R

A

A

R

R

M

R

G

G

M

I

G

G

F

Y

V

L

P

P

E

Q

D

E

R

A

R

S

V

I

F

F

A

R

D

R

L

L

T

S

V

V

M

Y

E

D

N

N

L

L

D

M

T

A

G

V

R

L

Q

Q

P

I

P

R

R

D

E

D

G

L

A

S

P

A

Q

E

W

Q

T

R

P

E

E

D

K

R

L

A

F

N

R

E

L

L

F

M

P

E

-

E

-

F

N

H

L

I

G

E

E

H

M

L

P

R

K

D

R

S

P

M

G

G

G

Q

Q

S

M

L

S

S

G

G

E

E

Q

R

M

R

L

V

T

E

V

I

S

A

R

R

T

A

L

L

M

A

G

A

N

N

P

P

Y

K

L

F

V

I

L

L

D

D

E

Q

P

P

S

F

E

A

G

G

V

V

A

D

P

P

V

I

I

S

V

V

E

I

Q

D

M

I

A

K

N

R

M

I

I

I

L

E

E

H

L

L

K

R

R

D

E

S

G

G

L

L

S

G

I

V

L

L

L

I

S

T

E

D

Q
x
H

N

N

L

V

H

R

F

E

A

T

E

L

L

A

V

V

S

C

D

E

R

R

A

A

Y

Y

V

I

L

V

E

S

K

Q

G

G

Q

H

I

L

R

I

F

A

S

H

G

G

T

T

I

P

G

T

E

E

L

I

A

L

S

Q

N

D

E

E

T

H

V

V

R

K

R

R

A

V

Y

Y

L

L

binding adenosine-5'-triphosphate: Y13 (≠ F14), R16 (= R17), V18 (≠ I20), N38 (= N40), G39 (= G41), G41 (= G43), K42 (= K44), T43 (≠ S45), T44 (= T46), H195 (≠ Q195)

6b8bA E. Coli lptb in complex with adp and a novobiocin derivative (see paper)
33% identity, 94% coverage: 13:234/237 of query aligns to 11:233/233 of 6b8bA

query
sites
6b8bA

A

A

F
x
Y

Y

K

G

G

R
|
R

A

-

H

R

I
x
V

L

V

F

E

D

D

V

V

G

S

L

L

E

T

V

V

G

N

R

S

G

G

E

E

V

I

V

V

A

G

L

L

M

L

G

G

R

P

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

T

T

M

F

K

Y

A

M

V

V

M

V

G

G

L

I

L

V

P

P

R

R

R

D

Q

A

G

G

E

N

V

I

I

I

F

I

R

D

G

D

Q

D

N

D

I

I

A

S

A

L

L

L

A

P

P

L

Y

H

K

A

I

R

A

A

R

R

M

R

G

G

M

I

G

G

F

Y

V

L

P

P

E

Q

D

E

R

A

R

S

V

I

F
|
F

A
x
R

D
x
R

L

L

T

S

V

V

M

Y

E

D

N

N

L

L

D

M

T

A

G

V

R

L

Q

Q

P

I

P

R

R

D

E

D

G

L

A

S

P

A

Q

E

W

Q

T

R

P

E

E

D

K

R

L

A

F

N

R

E

L

L

F

M

P

E

-

E

-

F

N

H

L

I

G

E

E

H

M

L

P

R

K

D

R

S

P

M

G

G

G

Q

Q

S

M

L

S

S

G

G

E

E

Q

R

M

R

L

V

T

E

V

I

S

A

R

R

T

A

L

L

M

A

G

A

N

N

P

P

Y

K

L

F

V

I

L

L

D

D

E

E

P

P

S

F

E

A

G

G

V

V

A

D

P

P

V

I

I

S

V

V

E

I

Q

D

M

I

A

K

N

R

M

I

I

I

L

E

E

H

L

L

K

R

R

D

E

S

G

G

L

L

S

G

I

V

L

L

L

I

S

T

E

D

Q

H

N

N

L

V

H

R

F

E

A

T

E

L

L

A

V

V

S

C

D

E

R

R

A

A

Y

Y

V

I

L

V

E

S

K

Q

G

G

Q

H

I

L

R

I

F

A

S

H

G

G

T

T

I

P

G

T

E

E

L

I

A

L

S

Q

N

D

E

E

T

H

V

V

R

K

R

R

A

V

Y

Y

binding adenosine-5'-diphosphate: Y12 (≠ F14), R15 (= R17), V17 (≠ I20), G38 (= G41), G40 (= G43), K41 (= K44), T42 (≠ S45), T43 (= T46)
binding (3s,5s,7s)-N-{7-[(3-O-carbamoyl-6-deoxy-5-methyl-4-O-methyl-beta-D-gulopyranosyl)oxy]-4-hydroxy-8-methyl-2-oxo-2H-1-benzopyran-3-yl}tricyclo[3.3.1.1~3,7~]decane-1-carboxamide: F89 (= F92), R90 (≠ A93), R91 (≠ D94)

1g6hA Crystal structure of the adp conformation of mj1267, an atp-binding cassette of an abc transporter (see paper)
31% identity, 91% coverage: 5:219/237 of query aligns to 4:232/254 of 1g6hA

query
sites
1g6hA

M

I

L

L

K

R

V

T

A

E

G

N

L

I

N

V

A

K

F

Y

Y
x
F

G

G

R

E

A
x
F

H

K

I

A

L

L

F

D

D

G

V

V

G

S

L

I

E

S

V

V

G

N

R

K

G

G

E

D

V

V

V

T

A

L

L

I

M

I

G

G

R

P

N
|
N

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

T

L

M

I

K

N

A

V

V

I

M

T

G

G

L

F

L

L

P

K

R

A

R

D

Q

E

G

G

E

R

V

V

I

Y

F

F

R

E

G

N

Q

K

N

D

I

I

A

T

A

N

L

K

A

E

P

P

Y

A

K

E

I

L

A

Y

R

H

M

Y

G

G

M

I

G

V

F

R

V

T

P

F

E

Q

D

T

R

P

R

Q

V

P

F

L

A

K

D

E

L

M

T

T

V

V

M

L

E

E

N

N

L

L

D

L

T

I

G

G

R

E

Q

I

P

C

P

P

R

G

E

E

G

S

-

P

-

L

-

N

-

S

-

L

-

F

A

Y

P

K

Q

K

W

W

T

I

P

P

-

K

-

E

-

M

-

V

E

E

K

K

L

A

F

F

R

K

L

I

-

L

-

E

F

F

P

L

N

K

L

L

G

S

E

H

M

L

P

Y

K

D

R

R

P

K

G

A

G

G

Q

E

M

L

S

S

G

G

E

Q

Q

M

Q

K

M

L

L

V

T

E

V

I

S

G

R

R

T

A

L

L

M

M

G

T

N

N

P

P

Y

K

L

M

V

I

L

V

L

M

D

D

E

E

P

P

S

I

E

A

G

G

V

V

A

A

P

P

V

G

I

L

V

A

E

H

Q

D

M

I

A

F

N

N

M

H

I

V

L

L

E

E

L

L

K

K

R

A

E

K

G

G

L

I

S

T

I

F

L

L

L

I

S

I

E

E

Q

H

N

R

L

L

H

D

F

I

A

V

E

L

L

N

V

Y

S

I

D

D

R

H

A

L

Y

Y

V

V

L

M

E

F

K

N

G

G

Q

Q

I

I

R

I

F

A

S

E

G

G

binding adenosine-5'-diphosphate: F14 (≠ Y15), F17 (≠ A18), N39 (= N40), G40 (= G41), G42 (= G43), K43 (= K44), S44 (= S45), T45 (= T46)

1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
31% identity, 91% coverage: 5:219/237 of query aligns to 4:232/253 of 1g9xB

query
sites
1g9xB

M

I

L

L

K

R

V

T

A

E

G

N

L

I

N

V

A

K

F

Y

Y
x
F

G

G

R

E

A
x
F

H

K

I

A

L

L

F

D

D

G

V

V

G

S

L

I

E

S

V

V

G

C

R

K

G

G

E

D

V

V

V

T

A

L

L

I

M

I

G

G

R

P

N

N

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

T

L

M

I

K

N

A

V

V

I

M

T

G

G

L

F

L

L

P

K

R

A

R

D

Q

E

G

G

E

R

V

V

I

Y

F

F

R

E

G

N

Q

K

N

D

I

I

A

T

A

N

L

K

A

E

P

P

Y

A

K

E

I

L

A

Y

R

H

M

Y

G

G

M

I

G

V

F

R

V

T

P

F

E

Q

D

T

R

P

R

Q

V

P

F

L

A

K

D

E

L

M

T

T

V

V

M

L

E

E

N

N

L

L

D

L

T

I

G

G

R

E

Q

I

P

N

P

P

R

G

E

E

G

S

-

P

-

L

-

N

-

S

-

L

-

F

A

Y

P

K

Q

K

W

W

T

I

P

P

-

K

-

E

-

M

-

V

E

E

K

K

L

A

F

F

R

K

L

I

-

L

-

E

F

F

P

L

N

K

L

L

G

S

E

H

M

L

P

Y

K

D

R

R

P

K

G

A

G

G

Q

E

M

L

S

S

G

G

E

Q

Q

M

Q

K

M

L

L

V

T

E

V

I

S

G

R

R

T

A

L

L

M

M

G

T

N

N

P

P

Y

K

L

M

V

I

L

V

L

M

D

D

E
|
E

P

P

S

I

E

A

G

G

V

V

A

A

P

P

V

G

I

L

V

A

E

H

Q

D

M

I

A

F

N

N

M

H

I

V

L

L

E

E

L

L

K

K

R

A

E

K

G

G

L

I

S

T

I

F

L

L

L

I

S

I

E

E

Q

H

N

R

L

L

H

D

F

I

A

V

E

L

L

N

V

Y

S

I

D

D

R

H

A

L

Y

Y

V

V

L

M

E

F

K

N

G

G

Q

Q

I

I

R

I

F

A

S

E

G

G

binding adenosine-5'-diphosphate: F14 (≠ Y15), F17 (≠ A18), G40 (= G41), S41 (≠ A42), G42 (= G43), K43 (= K44), S44 (= S45), T45 (= T46)
binding magnesium ion: S44 (= S45), E176 (= E163)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
28% identity, 98% coverage: 4:236/237 of query aligns to 1:237/241 of 4u00A

query
sites
4u00A

P

P

M

I

L

I

K

R

V

I

A

R

G

N

L

L

N

H

A

K

F

W

Y
x
F

G

G

R

P

A

L

H

H

I
x
V

L

L

F

K

D

G

V

I

G

H

L

L

E

E

V

V

G

A

R

P

G

G

E

E

V

K

V

L

A

V

L

I

M

I

G

G

R

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

T

L

M

I

K

R

A

T

V

I

M

N

G

R

L

L

L

E

P

D

R

F

R

Q

Q

E

G

G

E

E

V

V

I

V

F

V

R

D

G

G

Q

L

N

S

I

V

A

K

A

D

L

D

A

R

P

A

Y

L

K

R

I

E

A

I

R

R

M

R

G

E

M

V

G

G

F

M

V

V

P

F

E

Q

D

Q

R

F

R

N

V

L

F

F

A

P

D

H

L

M

T

T

V

V

M

L

E

E

N

N

L

V

D

T

T

L

G

A

-

P

-

M

-

R

-

V

R

R

Q

R

P

W

P

P

R

R

E

E

G

K

A

A

P

E

Q

K

W

K

T

A

P

L

E

E

K

L

L

L

F

E

R

R

L

V

F

-

P

-

N

G

L

I

G

L

E

D

M

Q

P

A

K

R

R

K

P

Y

G

P

G

A

Q

Q

M

L

S

S

G

G

E

Q

Q

Q

M

R

L

V

T

A

V

I

S

A

R

R

T

A

L

L

M

A

G

M

N

E

P

P

Y

K

L

I

V

M

L

L

L

F

D

D

E

E

P

P

S

T

E

S

G

A

V

L

A

D

P

P

V

E

I

M

V

V

E

G

Q

E

M

V

A

L

N

D

M

V

I

M

L

R

E

D

L

L

K

A

R

Q

E

G

G

G

L

M

S

T

I

M

L

V

S

V

E

T

Q

H

N

E

L

M

H

G

F

F

A

A

E

R

L

E

V

V

S

A

D

D

R

R

A

V

Y

V

V

F

L

M

E

D

K

G

G

G

Q

Q

I

I

R

V

F

E

S

E

G

G

T

R

I

P

G

E

E

E

L

I

A

F

S

T

-

R

-

P

N

K

E

E

T

E

V

R

R

T

R

R

A

S

Y

F

L

L

S

Q

binding adenosine-5'-diphosphate: F12 (≠ Y15), V17 (≠ I20), S37 (≠ N40), G38 (= G41), S39 (≠ A42), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46)

Q5SSE9 ATP-binding cassette sub-family A member 13; EC 7.6.2.- from Mus musculus (Mouse) (see paper)
32% identity, 82% coverage: 23:216/237 of query aligns to 3828:4021/5034 of Q5SSE9

query
sites
Q5SSE9

D

E

V

L

G

T

L

L

E

T

V

F

G

H

R

R

G

D

E

Q

V

I

V

T

A

A

L

L

M

L

G

G

R

T

N

N

G

G

A

A

G

G

K
|
K

S

T

T

T

T

I

M

I

K

S

A

M

V

L

M

M

G

G

L

L

L

F

P

P

R

P

R

T

Q

S

G

G

E

T

V

I

I

T

F

I

R

N

G

G

Q

K

N

N

I

L

A

Q

A

-

L

-

A

T

P

D

Y

L

K

S

I

K

A

V

R

R

M

E

G

E

M

L

G

G

F

V

V

C

P

P

E

Q

D

Q

R

D

R

V

V

L

F

L

A

D

D

N

L

L

T

T

V

V

M

R

E

E

N

H

L

L

D

M

T

L

G

F

R

A

Q

S

P

I

P

K

R

-

E

-

G

-

A

A

P

P

Q

W

W

W

T

T

P

T

E

K

K

E

L

L

F

Q

R

Q

L

Q

F

V

P

N

N

K

-

T

-

L

-

D

-

E

-

V

-

E

L

L

G

T

E

Q

M

H

P

Q

K

H

R

K

P

P

G

A

G

G

Q

V

M

L

S

S

G

G

E

M

Q

K

Q

R

M

K

L

L

T

S

V

I

S

G

R

I

T

A

L

F

M

M

G

G

N

M

P

S

Y

K

L

T

V

V

L

V

L

L

D

D

E

E

P

P

S

S

E

S

G

G

V

V

A

D

P

P

V

C

I

S

V

R

E

R

Q

S

M

L

A

W

N

D

M

I

I

L

L

L

E

K

L

Y

K

-

R

R

E

E

G

G

L

R

S

T

I

I

L

I

L

F

S

T

E
x
T

Q

H

N

H

L

L

H

D

F

E

A

A

E

E

L

M

V

L

S

S

D

D

R

H

A

V

Y

A

V

V

L

L

E

Q

K

Q

G

G

Q

R

I

L

R

R

K3849 (= K44) mutation to M: Does not affect intracellular vesicle localization. Affects cholesterol internalization.
T3999 (≠ E194) mutation to A: Does not affect protein expression. Affects intracellular vesicles localization. Impairs intracellular cholesterol accumulation in the vesicle.

Sites not aligning to the query:

3577 H→P: Does not affect protein expression. Decreases intracellular cholesterol accumulation in the vesicle.
4735 K→M: Does not affect intracellular vesicle localization.Affects cholesterol internalization.
4818 R→C: Does not affect protein expression. Decreases intracellular cholesterol accumulation in the vesicle.

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
29% identity, 98% coverage: 5:236/237 of query aligns to 1:237/240 of 4ymuJ

query
sites
4ymuJ

M

M

L

I

K

F

V

V

A

N

G

D

L

V

N

Y

A

K

F

N

Y
x
F

G

G

R

S

A

L

H

E

I
x
V

L

L

F

K

D

G

V

V

G

T

L

L

E

K

V

V

G

N

R

K

G

G

E

E

V

V

A

V

L

I

M

I

G

G

R

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

T

L

M

L

K

R

A

C

V

I

M

N

G

L

L

L

L

E

P

E

R

P

R

T

Q

K

G

G

E

E

V

V

I

F

F

I

R

D

G

G

Q

V

N

K

I

I

A

N

-

N

A

G

L

K

A

V

P

N

Y

I

K

N

I

K

A

V

R

R

M

Q

G

K

M

V

G

G

F

M

V

V

P

F

E

Q

D

H

R

F

R

N

V

L

F

F

A

P

D

H

L

L

T

T

V

A

M

I

E

E

N

N

L

I

D

T

T

L

G

A

R

P

Q

V

P

K

P

V

R

K

E

K

G

M

A

N

P

K

Q

K

W

E

T

A

P

E

E

E

K

L

L

A

F

V

R

D

L

L

F

L

P

A

N

K

L

V

G

G

E

L

M

L

P

D

K

K

R

K

P

D

G

Q

G

Y

-

P

-

I

Q

K

M

L

S

S

G

G

E

Q

Q

K

Q

Q

M

R

L

L

T

A

V

I

S

A

R

R

T

A

L

L

M

A

G

M

N

Q

P

P

Y

E

L

V

V

M

L

L

L

F

D

D

E
|
E

P

P

S

T

E

S

G

A

V

L

A

D

P

P

V

E

I

M

V

V

E

K

Q

E

M

V

A

L

N

N

M

V

I

M

L

K

E

Q

L

L

K

A

R

N

E

E

G

G

L

M

S

T

I

M

L

V

S

V

E

T

Q
x
H

N

E

L

M

H

G

F

F

A

A

E

R

L

E

V

V

S

G

D

D

R

R

A

V

Y

I

V

F

L

M

E

D

K

D

G

G

Q

V

I

I

R

V

F

E

S

E

G

G

T

T

I

P

G

E

E

E

L

I

-

F

-

Y

-

R

A

A

S

K

N

N

E

E

T

R

V

T

R

R

R

E

A

-

Y

F

L

L

S

S

binding adenosine-5'-triphosphate: F11 (≠ Y15), V16 (≠ I20), S36 (≠ N40), G37 (= G41), S38 (≠ A42), G39 (= G43), K40 (= K44), S41 (= S45), T42 (= T46), E162 (= E163), H194 (≠ Q195)
binding magnesium ion: S41 (= S45), E162 (= E163)

8ee6A Cryo-em structure of human abca7 in pe/ch nanodiscs (see paper)
32% identity, 80% coverage: 31:220/237 of query aligns to 1525:1713/1808 of 8ee6A

query
sites
8ee6A

G

G

E

E

V

C

V

F

A

G

L

L

M

L

G

G

R

V

N

N

G
|
G

A
|
A

G
|
G

K
|
K

S
x
T

T

S

T

T

M

F

K

R

A

M

V

V

M

T

G

G

L

D

L

T

P

L

R

A

R

S

Q

R

G

G

E

E

V

A

I

V

F

L

R

A

G

G

Q

H

N

S

I

V

A

A

A

R

L

E

A

P

P

-

Y

-

K

S

I

A

A

A

R

H

M

L

G

S

M

M

G

G

F

Y

V

C

P

P

E

Q

D

S

R

D

R

A

V

I

F

F

A

E

D

L

L

L

T

T

V

G

M

R

E

E

N

H

L

L

D

E

T

L

G

L

-

A

-

R

-

L

R

R

Q

G

P

V

P

P

R

E

E

A

G

Q

A

V

P

A

Q

Q

W

T

T

A

P

G

E

S

K

G

L

L

F

A

R

R

L

L

F

-

P

-

N

G

L

L

G

S

E

W

M

Y

P

A

K

D

R

R

P

P

G

A

G

G

Q

T

M

Y

S

S

G

G

E

N

Q

K

Q

R

M

K

L

L

T

A

V

T

S

A

R

L

T

A

L

L

M

V

G

G

N

D

P

P

Y

A

L

V

V

V

L

F

L

L

D

D

E

E

P

P

S

T

E

T

G

G

V

M

A

D

P

P

V

S

I

A

V

R

E

R

Q

F

M

L

A

W

N

N

M

S

I

L

L

L

E

A

L

V

K

V

R

R

E

E

G

G

L

R

S

S

I

V

L

M

L

L

S

T

E

S

Q

H

N

S

L

M

H

E

F

E

A

C

E

E

L

A

V

L

S

C

D

S

R

R

A

L

Y

A

V

I

L

M

E

V

K

N

G

G

Q

R

I

F

R

R

F

C

S

L

G

G

T

S

binding phosphothiophosphoric acid-adenylate ester: G1535 (= G41), A1536 (= A42), G1537 (= G43), K1538 (= K44), T1539 (≠ S45)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 607, 608, 633, 640, 660, 661, 663, 664, 665, 666, 1507, 1514

P55339 ABC-type transporter ATP-binding protein EcsA from Bacillus subtilis (strain 168) (see paper)
31% identity, 98% coverage: 5:236/237 of query aligns to 3:233/247 of P55339

query
sites
P55339

M

L

L

L

K

S

V

V

A

K

G

D

L

L

N

T

A

G

F

G

Y

Y

G

T

R

R

A

N

H

P

I

V

L

L

F

K

D

N

V

V

G

S

L

F

E

T

V

L

G

E

R

P

G

N

E

Q

V

I

V

V

A

G

L

L

M

I

G

G

R

L

N

N

G

G

A

A

G

G

K

K

S

S

T

T

M

I

K

R

A

H

V

I

M

I

G

G

L

L

L

M

P

D

R

P

R

H

Q

K

G

G

E

S

V

I

I

E

F

L

R

N

G

G

Q

K

N

T

I

F

A

A

A

E

L

-

A

D

P

P

Y

-

K

E

I

G

A

Y

R

R

M

S

G

Q

M

F

G

T

F

Y

V

I

P

P

E

E

D

T

R

P

R

V

V

L

F

Y

A

E

D

E

L

L

T

T

V

L

M

M

E

E

N

H

L

L

D

E

T

L

G

T

R

A

Q

M

P

A

P

-

R

-

E

Y

G

G

A

L

P

S

Q

K

W

E

T

T

P

M

E

E

K

K

L

-

F

-

R

R

L

L

F

P

P

P

N

L

L

L

G

K

E

E

-

F

-

R

M

M

P

E

K

K

R

R

P

L

G

K

-

W

-

F

-

P

G

A

Q

H

M

F

S

S

G

K

G

G

E

M

Q

K

Q

Q

M

K

L

V

T

M

V

I

S

M

R

C

T

A

L

F

M

L

G

A

N

E

P

P

Y

A

L

L

V

Y

L

I

D

D

E

E

P

P

S

F

E

L

G
|
G

V

L

A

D

P

P

V

L

I

A

V

I

E

N

Q

A

M

L

A

L

N

E

M

R

I

M

L

N

E

E

L

A

K

K

R

K

E

G

G

G

L

A

S

S

I

V

L

L

L

M

S

S

E

T

Q

H

N

I

L

L

H

A

F

T

A

A

E

E

L

R

V

Y

S

C

D

D

R

S

A

F

Y

I

V

I

L

L

E

H

K

N

G

G

Q

E

I

V

R

R

F

A

S

R

G

G

T

T

I

L

G

S

E

E

L

L

A

R

S

E

N

Q

E

F

T

G

V

M

R

K

R

D

A

A

Y

A

L

L

S

D

G164 (= G167) mutation to E: In ecsA-26; exoamylase and other exoenzymes decrease, competence- and sporulation-deficient phenotype.

Q9BZC7 ATP-binding cassette sub-family A member 2; ATP-binding cassette transporter 2; ATP-binding cassette 2; EC 7.6.2.- from Homo sapiens (Human) (see paper)
28% identity, 93% coverage: 5:224/237 of query aligns to 2049:2272/2435 of Q9BZC7

query
sites
Q9BZC7

M

M

L

V

K

K

V

I

A

E

G

N

L

L

N

T

A

K

F

V

Y

Y

-

K

-

S

-

R

-

K

-

I

G

G

R

R

A

I

H

L

I

A

L

V

F

D

D

R

V

L

G

C

L

L

E

G

V

V

G

R

R

P

G

G

E

E

V

C

V

F

A

G

L

L

M

L

G

G

R

V

N

N

G

G

A

A

G

G

K

K

S

T

T

S

T

T

M

F

K

K

A

M

V

L

M

T

G

G

L

D

L

E

P

S

R

T

Q

G

G

G

E

E

V

A

I

F

F

V

R

N

G

G

Q

H

N

S

I

V

A

-

L

L

A

K

P

E

Y

L

K

L

I

Q

A

V

R

Q

M

Q

G

S

M

L

G

G

F

Y

V

C

P

P

E

Q

D

C

R

D

R

A

V

L

F

F

A

D

D

E

L

L

T

T

V

A

M

R

E

E

N

H

L

L

D

Q

-

L

-

Y

-

T

-

R

-

L

-

R

-

G

-

I

T

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G

W

R

K

Q

D

P

E

P

A

R

R

E

-

G

-

A

V

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W

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A

P

L

E

E

K

K

L

L

F

-

R

-

L

-

F

-

P

-

N

E

L

L

G

T

E

K

M

Y

P

A

K

D

R

K

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P

G

A

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T

M

Y

S

S

G

G

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Q

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S

A

R

I

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A

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M

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G

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Y

P

P

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A

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F

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I

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F

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D

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M

A

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M

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N

N

M

L

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D

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G

G

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S

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M

H

E

F

E

A

C

E

E

L

A

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L

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C

D

T

R

R

A

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Y

A

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L

M

E

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G

G

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I

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L

G

G

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S

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I

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E

H

L

L

Sites not aligning to the query:

271 modified: N5-methylglutamine; Q→R: Abolishes methylation by N6AMT1.

8eopA Cryo-em structure of nanodisc reconstituted human abca7 eq mutant in atp bound closed state (see paper)
31% identity, 80% coverage: 31:220/237 of query aligns to 1479:1667/1687 of 8eopA

query
sites
8eopA

G

G

E

E

V

C

V

F

A

G

L

L

M

L

G

G

R

V

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
x
S

T

T

M

F

K

R

A

M

V

V

M

T

G

G

L

D

L

T

P

L

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A

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R

G

G

E

E

V

A

I

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F

L

R

A

G

G

Q

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N

S

I

V

A

A

A

R

L

E

A

P

P

-

Y

-

K

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I

A

A

A

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H

M

L

G

S

M

M

G

G

F

Y

V

C

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E
x
Q

D

S

R

D

R

A

V

I

F

F

A

E

D

L

L

L

T

T

V

G

M

R

E

E

N

H

L

L

D

E

T

L

G

L

-

A

-

R

-

L

R

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G

P

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P

P

R

E

E

A

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Q

A

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A

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T

T

A

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G

E

S

K

G

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R

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-

P

-

N

G

L

L

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W

M

Y

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A

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P

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x
T

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S

G

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N

Q

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M

K

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A

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G

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D

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A

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G

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M

A

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M

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N

N

M

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E

G

G

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S

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M

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C

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A

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G

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S

binding adenosine-5'-triphosphate: N1488 (= N40), G1489 (= G41), G1491 (= G43), K1492 (= K44), S1494 (≠ T46), Q1533 (≠ E87), T1584 (≠ Q137), S1586 (= S139)
binding magnesium ion: T1493 (≠ S45), Q1533 (≠ E87)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 659, 662, 666, 686, 687, 689, 690, 691, 692, 782, 784, 1461

E9Q876 Glucosylceramide transporter ABCA12; ATP-binding cassette sub-family A member 12; EC 7.6.2.1 from Mus musculus (Mouse) (see 2 papers)
30% identity, 89% coverage: 10:220/237 of query aligns to 1350:1560/2595 of E9Q876

query
sites
E9Q876

G

G

L

V

N

T

A

K

F

I

Y

Y

G

G

R

S

A

K

H

T

I

A

L

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F

E

D

N

V

L

G

N

L

L

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N

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G

G

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H

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M

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N

N

G

G

A

A

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G

K

K

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T

T

M
x
I

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x
S

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x
M

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x
L

M
x
T

G
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G

L
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L

L
x
F

P
x
G

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x
A

R
x
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x
A

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G

E
x
T

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x
I

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F

F
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x
Y

G
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G

Q
x
K

N
x
D

I
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I

A
x
K

A

-

L

-

A
x
T

P
x
D

Y
x
L

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x
N

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x
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R

M
x
K

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M
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M

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G

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x
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V
x
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x
M

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x
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x
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x
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x
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F

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x
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x
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L

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x
T

M
x
K

E
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E

N
x
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L

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x
L

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x
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x
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x
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W

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x
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L

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x
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E

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R

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S

1388:1461 (vs. 48:122, 23% identical) mutation to M: In a mouse model for harlequin ichthyosis (HI), smooth skin (smsk) mutant mice show a pronounced perinatal lethal skin phenotype in 25% of the offspring and newborn mutant pups die within a few hours after birth, and appear severely dehydrated with dry cracking skin. Smsk homozygous mutants embryos show a normal appearance at 14.5 dpc, but at 16.5 dpc develop a partial absence of normal skin folds around the trunk and limbs, and by 18.5 dpc develop a taut, thick skin and limb contractures.

Sites not aligning to the query:

1996 G→D: In a mouse model for harlequin ichthyosis (HI), homozygous mice are embryonic lethal but occasionally pups are found in the first few hours after birth but die and are severely dehydrated and fail to suckle normally. Homozygous pups show hallmarks of HI desease including hyperkeratosis, abnormal extracellular lipid lamellae and defects in cornified envelope processing. At 14.5 dpc and 15.5 dpc homozygous embryos appear normal; however from 16.5 dpc onwards they are characterized by an absence of normal skin folds around the trunk and limbs. As development progressed, embryos develop a taut, thick epidermis and multiple contractures affecting the limbs. Late stage embryos are smaller.

8f5bA Human abca4 structure in complex with amp-pnp
31% identity, 80% coverage: 32:220/237 of query aligns to 749:935/1924 of 8f5bA

query
sites
8f5bA

E

Q

V

I

V

T

A

A

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M

L

G

G

R

H

N
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N

G
|
G

A

A

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G

K
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K

S
x
T

T
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T

T

T

M

L

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G

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N

D

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I

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A

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x
Q

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R

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-

L

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E

N

D

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G

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-

N

-

E

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G

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Q
x
D

M

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G

G

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M

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M

K

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G

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D

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D

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S

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H

N

H

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M

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D

F

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A

A

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D

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D

D

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K

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G

G

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T

binding phosphoaminophosphonic acid-adenylate ester: N757 (= N40), G758 (= G41), G760 (= G43), K761 (= K44), T762 (≠ S45), T763 (= T46), Q802 (≠ E87), D853 (≠ Q137)
binding magnesium ion: T762 (≠ S45), Q802 (≠ E87)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 1652, 1653, 1654, 1655, 1656, 1657, 1697, 1748, 1750
binding magnesium ion: 1657, 1697

Query Sequence

>H281DRAFT_01585 H281DRAFT_01585 amino acid/amide ABC transporter ATP-binding protein 2, HAAT family
MSEPMLKVAGLNAFYGRAHILFDVGLEVGRGEVVALMGRNGAGKSTTMKAVMGLLPRRQG
EVIFRGQNIAALAPYKIARMGMGFVPEDRRVFADLTVMENLDTGRQPPREGAPQWTPEKL
FRLFPNLGEMPKRPGGQMSGGEQQMLTVSRTLMGNPYLVLLDEPSEGVAPVIVEQMANMI
LELKREGLSILLSEQNLHFAELVSDRAYVLEKGQIRFSGTIGELASNETVRRAYLSV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory