SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_01586 H281DRAFT_01586 amino acid/amide ABC transporter ATP-binding protein 1, HAAT family to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1g6hA Crystal structure of the adp conformation of mj1267, an atp-binding cassette of an abc transporter (see paper)
34% identity, 91% coverage: 1:244/269 of query aligns to 2:253/254 of 1g6hA

query
sites
1g6hA

M

M

S

E

L

I

L

L

H

R

V

T

S

E

G

N

L

I

C

V

K

K

S

Y

F
|
F

G

G

G

E

L
x
F

K

K

A

A

V

L

D

D

D

G

V

V

S

S

F

I

T

S

L

V

E

N

A

K

G

G

Q

D

L

V

L

T

A

L

L

I

G

G

P

P

N
|
N

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

C

L

F

I

N

N

M

V

V

I

N

T

G

G

Q

F

L

L

S

K

P

A

S

D

S

E

G

G

S

R

I

V

R

Y

F

F

D

E

G

N

H

K

E

D

L

I

V

T

G

N

M

K

R

E

P

P

R

A

D

E

I

L

W

Y

R

H

L

Y

G

G

V

I

G

V

R

R

T

T

F

F

Q

Q

I

T

A

P

A

Q

T

P

F

L

N

K

S

E

M

M

T

T

V

V

L

L

E

E

N

N

V

L

Q

L

M

I

A

G

L

E

V

I

S

C

R

P

E

G

R

E

K

S

T

P

-

L

-

N

-

S

-

L

-

F

F

Y

G

K

V

K

W

W

K

I

P

P

A

K

A

E

S

E

R

E

Y

M

A

V

D

E

E

K

A

A

F

F

A

K

L

I

L

L

E

E

Q

F

V

L

G

K

M

L

A

S

A

H

D

L

A

Y

N

D

R

R

A

K

C

A

G

G

V

E

L

L

A

S

Y

G

G

G

D

Q

V

M

K

K

R

L

V

V

E

E

L

I

A

G

I

R

A

A

L

L

A

M

N

T

R

N

P

P

K

K

L

M

L

I

L

V

M

M

D

D

E

E

P

P

T

I

A

A

G

G

M

V

A

A

P

P

K

G

E

L

R

A

N

H

E

D

L

I

M

F

A

N

L

H

T

V

K

L

R

E

L

L

V

-

T

K

E

A

Q

K

K

G

I

I

G

T

V

F

L

L

F

I

T

I

E

E

H

H

S

R

M

L

D

D

V

I

V

V

F

L

A

N

Y

Y

A

I

D

D

R

H

M

L

I

Y

V

V

L

M

A

F

R

N

G

G

K

Q

L

I

I

I

A

A

E

E

G

G

D

R

G

G

-

E

D

E

T

E

I

I

R

K

N

N

-

V

-

L

-

S

D

D

P

P

R

K

V

V

Q

V

E

E

V

I

Y

Y

F

I

G

G

binding adenosine-5'-diphosphate: F14 (= F13), F17 (≠ L16), N39 (= N38), G40 (= G39), G42 (= G41), K43 (= K42), S44 (= S43), T45 (= T44)

1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
34% identity, 91% coverage: 1:244/269 of query aligns to 2:253/253 of 1g9xB

query
sites
1g9xB

M

M

S

E

L

I

L

L

H

R

V

T

S

E

G

N

L

I

C

V

K

K

S

Y

F
|
F

G

G

G

E

L
x
F

K

K

A

A

V

L

D

D

D

G

V

V

S

S

F

I

T

S

L

V

E

C

A

K

G

G

Q

D

L

V

L

T

A

L

L

I

G

G

P

P

N

N

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

C

L

F

I

N

N

M

V

V

I

N

T

G

G

Q

F

L

L

S

K

P

A

S

D

S

E

G

G

S

R

I

V

R

Y

F

F

D

E

G

N

H

K

E

D

L

I

V

T

G

N

M

K

R

E

P

P

R

A

D

E

I

L

W

Y

R

H

L

Y

G

G

V

I

G

V

R

R

T

T

F

F

Q

Q

I

T

A

P

A

Q

T

P

F

L

N

K

S

E

M

M

T

T

V

V

L

L

E

E

N

N

V

L

Q

L

M

I

A

G

L

E

V

I

S

N

R

P

E

G

R

E

K

S

T

P

-

L

-

N

-

S

-

L

-

F

F

Y

G

K

V

K

W

W

K

I

P

P

A

K

A

E

S

E

R

E

Y

M

A

V

D

E

E

K

A

A

F

F

A

K

L

I

L

L

E

E

Q

F

V

L

G

K

M

L

A

S

A

H

D

L

A

Y

N

D

R

R

A

K

C

A

G

G

V

E

L

L

A

S

Y

G

G

G

D

Q

V

M

K

K

R

L

V

V

E

E

L

I

A

G

I

R

A

A

L

L

A

M

N

T

R

N

P

P

K

K

L

M

L

I

L

V

M

M

D

D

E
|
E

P

P

T

I

A

A

G

G

M

V

A

A

P

P

K

G

E

L

R

A

N

H

E

D

L

I

M

F

A

N

L

H

T

V

K

L

R

E

L

L

V

-

T

K

E

A

Q

K

K

G

I

I

G

T

V

F

L

L

F

I

T

I

E

E

H

H

S

R

M

L

D

D

V

I

V

V

F

L

A

N

Y

Y

A

I

D

D

R

H

M

L

I

Y

V

V

L

M

A

F

R

N

G

G

K

Q

L

I

I

I

A

A

E

E

G

G

D

R

G

G

-

E

D

E

T

E

I

I

R

K

N

N

-

V

-

L

-

S

D

D

P

P

R

K

V

V

Q

V

E

E

V

I

Y

Y

F

I

G

G

binding adenosine-5'-diphosphate: F14 (= F13), F17 (≠ L16), G40 (= G39), S41 (≠ A40), G42 (= G41), K43 (= K42), S44 (= S43), T45 (= T44)
binding magnesium ion: S44 (= S43), E176 (= E170)

6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
32% identity, 90% coverage: 2:244/269 of query aligns to 1:235/240 of 6mjpA

query
sites
6mjpA

S

A

L

I

L

L

H

K

V

A

S

Q

G

H

L

L

C

A

K

K

S

S

F

Y

G

K

L

R

K

K

A

V

V

V

D

S

D

D

V

V

S

S

F

L

T

Q

L

V

E

E

A

S

G

G

Q

Q

L

I

L

V

A

G

L

L

G

G

P

P

N

N

G

G

A

A

G

G

K

K

S

T

T

T

C

S

F

F

N

Y

M

M

V

I

N

V

G

G

Q

L

L

V

S

A

P

R

S

D

S

E

G

G

S

T

I

I

R

T

F

I

D

D

G

D

H

N

E

D

L

I

V

S

G

I

M

L

R

P

P

M

R

H

D

S

I

R

W

S

R

R

L

M

G

G

V

I

G

G

R

Y

T

L

F

P

Q

Q

I

E

A

A

A

S

T

I

F

F

N

R

S

K

M

L

T

S

V

V

L

E

E

D

N

N

V

I

Q

M

M

A

A

V

L

L

V

Q

S

T

R

R

E

E

R

E

K

L

T

T

F

H

G

-

V

-

W

-

K

-

P

-

A

-

S
x
E

R

E

Y

R

A

Q

D
|
D

E

K

A

L

F

E

A

D

L

L

E

E

Q

E

V

F

G

H

M

I

A

Q

A

H

D

I

A

R

N

K

R

S

A

A

C

G

G

M

V

A

L

L

A

S

Y

G

G

G

D

E

V

R

K

R

R

R

V

V

E

E

L

I

A

A

I

R

A

A

L

L

A

A

N

A

R

N

P

P

K

Q

L

F

L

I

L

L

M

L

D

D

E

Q

P

P

T

F

A

A

G

G

M

V

A

D

P

P

K

I

E

S

R

V

N

I

E

D

L

I

M

K

A

K

L

I

T

I

K

E

R

H

L

L

V

-

T

R

E

D

Q

R

K

G

I

L

G

G

V

V

L

L

F

I

T

T

E

D

H

H

S

N

M

V

D

R

V

E

V

T

F

L

A

D

Y

V

A

C

D

E

R

K

M

A

I

Y

V

I

L

V

A

S

R

Q

G

G

K

R

L

L

I

I

A

A

E

E

G

G

D

T

G

P

D

Q

T

D

I

V

R

L

N

N

D

N

P

E

R

Q

V

V

Q

K

E

Q

V

V

Y

Y

F

L

G

G

binding calcium ion: E111 (≠ S119), D115 (= D123)

6s8nB Cryo-em structure of lptb2fgc in complex with lipopolysaccharide (see paper)
33% identity, 90% coverage: 4:244/269 of query aligns to 3:235/238 of 6s8nB

query
sites
6s8nB

L

L

H

T

V

A

S

K

G

N

L

L

C

A

K

K

S

A

F

Y

G

K

G

G

L

R

K

R

A

V

V

V

D

E

D

D

V

V

S

S

F

L

T

T

L

V

E

N

A

S

G

G

Q

E

L

I

L

V

A

G

L

L

G

G

P

P

N

N

G

G

A

A

G

G

K

K

S

T

T

T

C

T

F

F

N

Y

M

M

V

V

N

V

G

G

Q

I

L

V

S

P

P

R

S

D

S

A

G

G

S

N

-

I

I

I

R

D

F

D

D

D

G

D

H

I

E

S

L

L

V

L

G

P

M

L

R

H

P

A

R

R

D

-

I

-

W

A

R

R

L

R

G

G

V

I

G

G

R

Y

T

L

F

P

Q

Q

I

E

A

A

A

S

T

I

F

F

N
x
R

S

R

M

L

T

S

V

V

L

Y

E

D

N

N

V

L

Q

M

M

A

A

V

L

L

V

Q

S

I

R

R

E

D

R

-

K

-

T

-

F

-

G

-

V

-

W

-

K

D

P

L

A

S

A

A

S

E

R

Q

Y

R

A

E

D

D

E

R

A

A

F

N

A

E

L

L

L

M

E

E

Q

E

V

F

G

H

M

I

A

E

A

H

D

L

A

R

N

D

R

S

A
x
M

C
x
G

G
x
Q

V

S

L

L

A

S

Y

G

G

G

D

E

V

R

K

R

R

R

V

V

E

E

L

I

A

A

I

R

A

A

L

L

A

A

N

A

R

N

P

P

K

K

L

F

L

I

L

L

M

L

D

D

E

E

P

P

T

F

A

A

G

G

M

V

A

D

P

P

K

I

E

S

R

V

N

I

E

D

L

I

M

K

A

R

L

I

T

I

K

E

R

H

L

L

V

-

T

R

E

D

Q

S

K

G

I

L

G

G

V

V

L

L

F

I

T

T

E

D

H

H

S

N

M

V

D

R

V

E

V

T

F

L

A

A

Y

V

A

C

D

E

R

R

M

A

I

Y

V

I

L

V

A

S

R

Q

G

G

K

H

L

L

I

I

A

A

E

H

G

G

D

T

G

P

D

T

T

E

I

I

R

L

N

Q

D

D

P

E

R

H

V

V

Q

K

E

R

V

V

Y

Y

F

L

G

G

binding 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione: R90 (≠ N91), M133 (≠ A141), G134 (≠ C142), Q135 (≠ G143)

6s8gA Cryo-em structure of lptb2fgc in complex with amp-pnp (see paper)
33% identity, 90% coverage: 4:244/269 of query aligns to 3:235/238 of 6s8gA

query
sites
6s8gA

L

L

H

T

V

A

S

K

G

N

L

L

C

A

K

K

S

A

F
x
Y

G

K

G

G

L
x
R

K

R

A

V

V

V

D

E

D

D

V

V

S

S

F

L

T

T

L

V

E

N

A

S

G

G

Q

E

L

I

L

V

A

G

L

L

G

G

P

P

N
|
N

G

G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

C

T

F

F

N

Y

M

M

V

V

N

V

G

G

Q

I

L

V

S

P

P

R

S

D

S

A

G

G

S

N

-

I

I

I

R

D

F

D

D

D

G

D

H

I

E

S

L

L

V

L

G

P

M

L

R

H

P

A

R

R

D

-

I

-

W

A

R

R

L

R

G

G

V

I

G

G

R

Y

T

L

F

P

Q
|
Q

I

E

A

A

A

S

T

I

F

F

N

R

S

R

M

L

T

S

V

V

L

Y

E

D

N

N

V

L

Q

M

M

A

A

V

L

L

V

Q

S

I

R

R

E

D

R

-

K

-

T

-

F

-

G

-

V

-

W

-

K

D

P

L

A

S

A

A

S

E

R

Q

Y

R

A

E

D

D

E

R

A

A

F

N

A

E

L

L

L

M

E

E

Q

E

V

F

G

H

M

I

A

E

A

H

D

L

A

R

N

D

R

S

A

M

C

G

G

Q

V
x
S

L

L

A
x
S

Y

G

G

G

D
x
E

V

R

K

R

R

R

V

V

E

E

L

I

A

A

I

R

A

A

L

L

A

A

N

A

R

N

P

P

K

K

L

F

L

I

L

L

M

L

D

D

E

E

P

P

T

F

A

A

G

G

M

V

A

D

P

P

K

I

E

S

R

V

N

I

E

D

L

I

M

K

A

R

L

I

T

I

K

E

R

H

L

L

V

-

T

R

E

D

Q

S

K

G

I

L

G

G

V

V

L

L

F

I

T

T

E

D

H

H

S

N

M

V

D

R

V

E

V

T

F

L

A

A

Y

V

A

C

D

E

R

R

M

A

I

Y

V

I

L

V

A

S

R

Q

G

G

K

H

L

L

I

I

A

A

E

H

G

G

D

T

G

P

D

T

T

E

I

I

R

L

N

Q

D

D

P

E

R

H

V

V

Q

K

E

R

V

V

Y

Y

F

L

G

G

binding phosphoaminophosphonic acid-adenylate ester: Y12 (≠ F13), R15 (≠ L16), N37 (= N38), G40 (= G41), K41 (= K42), T42 (≠ S43), T43 (= T44), Q84 (= Q85), S136 (≠ V144), S138 (≠ A146), E141 (≠ D149)

6mhzA Vanadate trapped cryo-em structure of e.Coli lptb2fg transporter (see paper)
33% identity, 90% coverage: 4:244/269 of query aligns to 3:235/235 of 6mhzA

query
sites
6mhzA

L

L

H

T

V

A

S

K

G

N

L

L

C

A

K

K

S

A

F
x
Y

G

K

G

G

L

R

K

R

A

V

V

V

D

E

D

D

V

V

S

S

F

L

T

T

L

V

E

N

A

S

G

G

Q

E

L

I

L

V

A

G

L

L

G

G

P

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

C

T

F

F

N

Y

M

M

V

V

N

V

G

G

Q

I

L

V

S

P

P

R

S

D

S

A

G

G

S

N

-

I

I

I

R

D

F

D

D

D

G

D

H

I

E

S

L

L

V

L

G

P

M

L

R

H

P

A

R

R

D

-

I

-

W

A

R

R

L

R

G

G

V

I

G

G

R

Y

T

L

F

P

Q
|
Q

I

E

A

A

A

S

T

I

F

F

N

R

S

R

M

L

T

S

V

V

L

Y

E

D

N

N

V

L

Q

M

M

A

A

V

L

L

V

Q

S

I

R

R

E

D

R

-

K

-

T

-

F

-

G

-

V

-

W

-

K

D

P

L

A

S

A

A

S

E

R

Q

Y

R

A

E

D

D

E

R

A

A

F

N

A

E

L

L

L

M

E

E

Q

E

V

F

G

H

M

I

A

E

A

H

D

L

A

R

N

D

R

S

A

M

C

G

G

Q

V
x
S

L

L

A
x
S

Y
x
G

G
|
G

D

E

V

R

K

R

R

R

V

V

E

E

L

I

A

A

I

R

A

A

L

L

A

A

N

A

R

N

P

P

K

K

L

F

L

I

L

L

M

L

D

D

E
|
E

P

P

T

F

A

A

G
|
G

M

V

A

D

P

P

K

I

E

S

R

V

N

I

E

D

L

I

M

K

A

R

L

I

T

I

K

E

R

H

L

L

V

-

T

R

E

D

Q

S

K

G

I

L

G

G

V

V

L

L

F

I

T

T

E

D

H
|
H

S

N

M

V

D

R

V

E

V

T

F

L

A

A

Y

V

A

C

D

E

R

R

M

A

I

Y

V

I

L

V

A

S

R

Q

G

G

K

H

L

L

I

I

A

A

E

H

G

G

D

T

G

P

D

T

T

E

I

I

R

L

N

Q

D

D

P

E

R

H

V

V

Q

K

E

R

V

V

Y

Y

F

L

G

G

binding adp orthovanadate: Y12 (≠ F13), N37 (= N38), G38 (= G39), G40 (= G41), K41 (= K42), T42 (≠ S43), T43 (= T44), Q84 (= Q85), S136 (≠ V144), S138 (≠ A146), G139 (≠ Y147), G140 (= G148), E162 (= E170), G166 (= G174), H194 (= H203)

6mbnA Lptb e163q in complex with atp (see paper)
33% identity, 90% coverage: 4:244/269 of query aligns to 4:236/241 of 6mbnA

query
sites
6mbnA

L

L

H

T

V

A

S

K

G

N

L

L

C

A

K

K

S

A

F
x
Y

G

K

G

G

L
x
R

K

R

A
x
V

V

V

D

E

D

D

V

V

S

S

F

L

T

T

L

V

E

N

A

S

G

G

Q

E

L

I

L

V

A

G

L

L

G

G

P

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

C

T

F

F

N

Y

M

M

V

V

N

V

G

G

Q

I

L

V

S

P

P

R

S

D

S

A

G

G

S

N

-

I

I

I

R

D

F

D

D

D

G

D

H

I

E

S

L

L

V

L

G

P

M

L

R

H

P

A

R

R

D

-

I

-

W

A

R

R

L

R

G

G

V

I

G

G

R

Y

T

L

F

P

Q

Q

I

E

A

A

A

S

T

I

F

F

N

R

S

R

M

L

T

S

V

V

L

Y

E

D

N

N

V

L

Q

M

M

A

A

V

L

L

V

Q

S

I

R

R

E

D

R

-

K

-

T

-

F

-

G

-

V

-

W

-

K

D

P

L

A

S

A

A

S

E

R

Q

Y

R

A

E

D

D

E

R

A

A

F

N

A

E

L

L

L

M

E

E

Q

E

V

F

G

H

M

I

A

E

A

H

D

L

A

R

N

D

R

S

A

M

C

G

G

Q

V

S

L

L

A

S

Y

G

G

G

D

E

V

R

K

R

R

R

V

V

E

E

L

I

A

A

I

R

A

A

L

L

A

A

N

A

R

N

P

P

K

K

L

F

L

I

L

L

M

L

D

D

E

Q

P

P

T

F

A

A

G

G

M

V

A

D

P

P

K

I

E

S

R

V

N

I

E

D

L

I

M

K

A

R

L

I

T

I

K

E

R

H

L

L

V

-

T

R

E

D

Q

S

K

G

I

L

G

G

V

V

L

L

F

I

T

T

E

D

H
|
H

S

N

M

V

D

R

V

E

V

T

F

L

A

A

Y

V

A

C

D

E

R

R

M

A

I

Y

V

I

L

V

A

S

R

Q

G

G

K

H

L

L

I

I

A

A

E

H

G

G

D

T

G

P

D

T

T

E

I

I

R

L

N

Q

D

D

P

E

R

H

V

V

Q

K

E

R

V

V

Y

Y

F

L

G

G

binding adenosine-5'-triphosphate: Y13 (≠ F13), R16 (≠ L16), V18 (≠ A18), N38 (= N38), G39 (= G39), G41 (= G41), K42 (= K42), T43 (≠ S43), T44 (= T44), H195 (= H203)

6b89A E. Coli lptb in complex with adp and novobiocin (see paper)
33% identity, 89% coverage: 4:243/269 of query aligns to 3:234/234 of 6b89A

query
sites
6b89A

L

L

H

T

V

A

S

K

G

N

L

L

C

A

K

K

S

A

F
x
Y

G

K

G

G

L
x
R

K

R

A
x
V

V

V

D

E

D

D

V

V

S

S

F

L

T

T

L

V

E

N

A

S

G

G

Q

E

L

I

L

V

A

G

L

L

G

G

P

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

C

T

F

F

N

Y

M

M

V

V

N

V

G

G

Q

I

L

V

S

P

P

R

S

D

S

A

G

G

S

N

-

I

I

I

R

D

F

D

D

D

G

D

H

I

E

S

L

L

V

L

G

P

M
x
L

R
x
H

P

A

R

R

D

-

I

-

W

A

R

R

L

R

G

G

V

I

G

G

R

Y

T

L

F
x
P

Q
|
Q

I

E

A
|
A

A
x
S

T

I

F
|
F

N
x
R

S
x
R

M
x
L

T

S

V

V

L

Y

E

D

N

N

V

L

Q

M

M

A

A
x
V

L

L

V

Q

S

I

R

R

E

D

R

-

K

-

T

-

F

-

G

-

V

-

W

-

K

D

P

L

A

S

A

A

S

E

R

Q

Y

R

A

E

D

D

E

R

A

A

F

N

A

E

L

L

L

M

E

E

Q

E

V

F

G

H

M

I

A

E

A

H

D

L

A

R

N

D

R

S

A

M

C

G

G
x
Q

V

S

L

L

A

S

Y

G

G

G

D

E

V

R

K

R

R

R

V

V

E

E

L

I

A

A

I
x
R

A

A

L

L

A

A

N

A

R

N

P

P

K

K

L

F

L

I

L

L

M

L

D

D

E

E

P

P

T

F

A

A

G

G

M

V

A

D

P

P

K

I

E

S

R

V

N

I

E

D

L

I

M

K

A

R

L

I

T

I

K

E

R

H

L

L

V

-

T

R

E

D

Q

S

K

G

I

L

G

G

V

V

L

L

F

I

T

T

E

D

H

H

S

N

M

V

D

R

V

E

V

T

F

L

A

A

Y

V

A

C

D

E

R

R

M

A

I

Y

V

I

L

V

A

S

R

Q

G

G

K

H

L

L

I

I

A

A

E

H

G

G

D

T

G

P

D

T

T

E

I

I

R

L

N

Q

D

D

P

E

R

H

V

V

Q

K

E

R

V

V

Y

Y

F

L

binding adenosine-5'-diphosphate: Y12 (≠ F13), R15 (≠ L16), V17 (≠ A18), N37 (= N38), G38 (= G39), G40 (= G41), K41 (= K42), T42 (≠ S43), T43 (= T44)
binding magnesium ion: T42 (≠ S43), Q84 (= Q85)
binding novobiocin: L71 (≠ M70), H72 (≠ R71), P83 (≠ F84), A86 (= A87), S87 (≠ A88), F89 (= F90), R90 (≠ N91), R91 (≠ S92), L92 (≠ M93), V101 (≠ A102), Q135 (≠ G143), R149 (≠ I157)

4p31A Crystal structure of a selenomethionine derivative of e. Coli lptb in complex with adp-magensium (see paper)
33% identity, 89% coverage: 4:243/269 of query aligns to 3:234/234 of 4p31A

query
sites
4p31A

L

L

H

T

V

A

S

K

G

N

L

L

C

A

K

K

S

A

F
x
Y

G

K

G

G

L
x
R

K

R

A
x
V

V

V

D

E

D

D

V

V

S

S

F

L

T

T

L

V

E

N

A

S

G

G

Q

E

L

I

L

V

A

G

L

L

G

G

P

P

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

C

T

F

F

N

Y

M

M

V

V

N

V

G

G

Q

I

L

V

S

P

P

R

S

D

S

A

G

G

S

N

-

I

I

I

R

D

F

D

D

D

G

D

H

I

E

S

L

L

V

L

G

P

M

L

R

H

P

A

R

R

D

-

I

-

W

A

R

R

L

R

G

G

V

I

G

G

R

Y

T

L

F

P

Q
|
Q

I

E

A

A

A

S

T

I

F

F

N

R

S

R

M

L

T

S

V

V

L

Y

E

D

N

N

V

L

Q

M

M

A

A

V

L

L

V

Q

S

I

R

R

E

D

R

-

K

-

T

-

F

-

G

-

V

-

W

-

K

D

P

L

A

S

A

A

S

E

R

Q

Y

R

A

E

D

D

E

R

A

A

F

N

A

E

L

L

L

M

E

E

Q

E

V

F

G

H

M

I

A

E

A

H

D

L

A

R

N

D

R

S

A

M

C

G

G

Q

V

S

L

L

A

S

Y

G

G

G

D

E

V

R

K

R

R

R

V

V

E

E

L

I

A

A

I

R

A

A

L

L

A

A

N

A

R

N

P

P

K

K

L

F

L

I

L

L

M

L

D

D

E

E

P

P

T

F

A

A

G

G

M

V

A

D

P

P

K

I

E

S

R

V

N

I

E

D

L

I

M

K

A

R

L

I

T

I

K

E

R

H

L

L

V

-

T

R

E

D

Q

S

K

G

I

L

G

G

V

V

L

L

F

I

T

T

E

D

H

H

S

N

M

V

D

R

V

E

V

T

F

L

A

A

Y

V

A

C

D

E

R

R

M

A

I

Y

V

I

L

V

A

S

R

Q

G

G

K

H

L

L

I

I

A

A

E

H

G

G

D

T

G

P

D

T

T

E

I

I

R

L

N

Q

D

D

P

E

R

H

V

V

Q

K

E

R

V

V

Y

Y

F

L

binding adenosine-5'-diphosphate: Y12 (≠ F13), R15 (≠ L16), V17 (≠ A18), G38 (= G39), G40 (= G41), K41 (= K42), T42 (≠ S43), T43 (= T44)
binding magnesium ion: T42 (≠ S43), Q84 (= Q85)

6b8bA E. Coli lptb in complex with adp and a novobiocin derivative (see paper)
33% identity, 89% coverage: 4:242/269 of query aligns to 3:233/233 of 6b8bA

query
sites
6b8bA

L

L

H

T

V

A

S

K

G

N

L

L

C

A

K

K

S

A

F
x
Y

G

K

G

G

L
x
R

K

R

A
x
V

V

V

D

E

D

D

V

V

S

S

F

L

T

T

L

V

E

N

A

S

G

G

Q

E

L

I

L

V

A

G

L

L

G

G

P

P

N

N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

C

T

F

F

N

Y

M

M

V

V

N

V

G

G

Q

I

L

V

S

P

P

R

S

D

S

A

G

G

S

N

-

I

I

I

R

D

F

D

D

D

G

D

H

I

E

S

L

L

V

L

G

P

M

L

R

H

P

A

R

R

D

-

I

-

W

A

R

R

L

R

G

G

V

I

G

G

R

Y

T

L

F

P

Q

Q

I

E

A

A

A

S

T

I

F
|
F

N
x
R

S
x
R

M

L

T

S

V

V

L

Y

E

D

N

N

V

L

Q

M

M

A

A

V

L

L

V

Q

S

I

R

R

E

D

R

-

K

-

T

-

F

-

G

-

V

-

W

-

K

D

P

L

A

S

A

A

S

E

R

Q

Y

R

A

E

D

D

E

R

A

A

F

N

A

E

L

L

L

M

E

E

Q

E

V

F

G

H

M

I

A

E

A

H

D

L

A

R

N

D

R

S

A

M

C

G

G

Q

V

S

L

L

A

S

Y

G

G

G

D

E

V

R

K

R

R

R

V

V

E

E

L

I

A

A

I

R

A

A

L

L

A

A

N

A

R

N

P

P

K

K

L

F

L

I

L

L

M

L

D

D

E

E

P

P

T

F

A

A

G

G

M

V

A

D

P

P

K

I

E

S

R

V

N

I

E

D

L

I

M

K

A

R

L

I

T

I

K

E

R

H

L

L

V

-

T

R

E

D

Q

S

K

G

I

L

G

G

V

V

L

L

F

I

T

T

E

D

H

H

S

N

M

V

D

R

V

E

V

T

F

L

A

A

Y

V

A

C

D

E

R

R

M

A

I

Y

V

I

L

V

A

S

R

Q

G

G

K

H

L

L

I

I

A

A

E

H

G

G

D

T

G

P

D

T

T

E

I

I

R

L

N

Q

D

D

P

E

R

H

V

V

Q

K

E

R

V

V

Y

Y

binding adenosine-5'-diphosphate: Y12 (≠ F13), R15 (≠ L16), V17 (≠ A18), G38 (= G39), G40 (= G41), K41 (= K42), T42 (≠ S43), T43 (= T44)
binding (3s,5s,7s)-N-{7-[(3-O-carbamoyl-6-deoxy-5-methyl-4-O-methyl-beta-D-gulopyranosyl)oxy]-4-hydroxy-8-methyl-2-oxo-2H-1-benzopyran-3-yl}tricyclo[3.3.1.1~3,7~]decane-1-carboxamide: F89 (= F90), R90 (≠ N91), R91 (≠ S92)

4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
32% identity, 83% coverage: 6:227/269 of query aligns to 7:218/285 of 4yerA

query
sites
4yerA

V

V

S

E

G

N

L

L

C

V

K

K

S

K

F
|
F

G

G

G

D

L
x
F

K

E

A

A

V

V

D

K

D

G

V

V

S

S

F

F

T

S

L

V

E

K

A

K

G

G

Q

E

L

I

L

F

A

A

L

F

L

L

G

G

P

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

C

T

F

I

N

H

M

M

V

L

N

T

G

T

Q

L

L

L

S

K

P

P

S

T

S

S

G

G

S

K

I

A

R

W

F

V

D

A

G

G

H

H

E

D

L

V

V

L

G

K

M

-

R

E

P

P

R

R

D

E

I

V

W

-

R

R

L

R

G

K

V

I

G

G

R

I

T

V

F

F

Q

Q

I

D

A

Q

A

S

T

L

F

D

N

R

S

E

M

L

T

T

V

A

L

Y

E

E

N

N

V

M

Q

Y

M

I

A

-

L

-

V

-

S

-

R

-

E

H

R

G

K

K

T

I

F

Y

G

G

V

Y

W

-

K

-

P

-

A

G

S

E

R

K

Y

L

A

K

D

K

E

R

A

I

F

L

A

E

L

L

E

E

Q

F

V

V

G

E

M

L

A

L

A

E

D

F

A

K

N

D

R

K

A

P

C

V

G

K

V
x
T

L
x
F

A
x
S

Y

G

G

G

D

M

V

A

K

R

R

R

V

L

E

E

L

I

A

A

I

R

A

S

L

L

A

I

N

H

R

E

P

P

K

E

L

V

L

L

L

F

M

L

D

D

E

E

P

P

T

T

A

I

G

G

M

L

A

D

P

P

K

H

E

T

R

R

N

A

E

H

L

M

M

W

A

E

L

Y

T

I

K

S

R

K

L

M

V

K

T

K

E

E

Q

H

K

N

I

M

G

T

V

I

L

F

F

L

T

T

E

T

H

H

S

Y

M

M

D

D

V

E

V

A

F

E

A

Q

Y

L

A

A

D

D

R

R

M

V

I

A

V

I

L

I

A

D

R

H

G

G

K

K

L

I

I

I

A

A

E

L

G

G

binding adenosine-5'-diphosphate: F14 (= F13), F17 (≠ L16), N39 (= N38), G40 (= G39), G42 (= G41), K43 (= K42), T44 (≠ S43), T45 (= T44), T135 (≠ V144), F136 (≠ L145), S137 (≠ A146)

5x40A Structure of a cbio dimer bound with amppcp (see paper)
33% identity, 87% coverage: 3:235/269 of query aligns to 4:230/280 of 5x40A

query
sites
5x40A

L

I

L

L

H

A

V

A

S

E

G

A

L

L

C

T

K

Y

S

A

F
|
F

-

P

G

G

L
x
V

K

K

A
|
A

V

L

D

D

V

L

S

S

F

L

T

A

L

V

E

P

A

K

G

G

Q

E

L

S

L

L

A

A

L

I

L

L

G

G

P

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

C

L

F

L

N

L

M

H

V

L

N

N

G

G

Q

T

L

L

S

R

P

P

S

Q

S

S

G

G

S

R

I

V

R

L

F

L

D

G

G

G

H

T

E

A

L

-

V

T

G

G

M

H

R

S

P

R

R

K

D

D

I

L

-

T

-

G

W

W

R

R

L

R

G

R

V

V

G

G

R

L

T

V

F

L

Q
|
Q

I

D

A

A

-

D

A

D

T

Q

F

L

N

F

S

A

M

T

T

T

V

V

L

F

E

E

N

D

V

V

Q

S

M

F

A

G

L

P

V

L

S

N

R

-

E

-

R

-

K

-

T

-

F

L

G

G

V

L

W

S

K

E

P

A

A

E

A

A

S

R

R

A

Y

R

A

V

D

E

E

E

A

A

F

L

A

A

L

A

L

L

E

S

Q

I

V

S

G

D

M

L

A

-

D

-

A

R

N

D

R

R

A

P

C

T

G
x
H

V
x
M

L
|
L

A
x
S

Y

G

G
|
G

D
x
Q

V

K

K

R

R

R

V

V

E

A

L

I

A

A

I

G

A

A

L

V

A

A

N

M

R

R

P

P

K

E

L

V

L

L

M

L

D

D

E
x
Q

P

P

T

T

A

A

G

G

M

L

A

D

P

L

K

A

E

G

R

T

N

E

E

Q

L

L

M

L

A

T

L

L

T

L

K

R

R

G

L

L

V

-

T

R

E

A

Q

A

K

G

I

M

G

T

V

L

L

V

F

F

T

S

E

T

H
|
H

S

D

M

V

D

E

V

L

V

A

F

A

A

A

Y

L

A

A

D

D

R

R

M

V

I

A

V

L

L

F

A

R

R

T

G

G

K

R

L

V

I

L

A

A

E

E

G

G

D

A

G

A

D

E

T

A

I

V

R

L

N

S

D

D

binding phosphomethylphosphonic acid adenylate ester: F14 (= F13), V18 (≠ L16), A20 (= A18), N40 (= N38), G41 (= G39), G43 (= G41), K44 (= K42), S45 (= S43), T46 (= T44), Q88 (= Q85), H139 (≠ G143), M140 (≠ V144), L141 (= L145), S142 (≠ A146), G144 (= G148), Q145 (≠ D149), Q166 (≠ E170), H198 (= H203)
binding magnesium ion: S45 (= S43), Q88 (= Q85)

A5U7B7 Cell division ATP-binding protein FtsE from Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) (see 2 papers)
31% identity, 84% coverage: 1:226/269 of query aligns to 2:219/229 of A5U7B7

query
sites
A5U7B7

M

I

S

T

L

L

L

D

H

H

V

V

S

T

G

K

L

Q

C

Y

K

K

S

S

F

-

G

S

G

A

L

R

K

P

A

A

V

L

D

D

V

I

S

N

F

V

T

K

L

I

E

D

A

K

G

G

Q

E

L

F

L

V

A

F

L

L

L

I

G

G

P

P

N

S

G

G

A

S

G

G

K
|
K

S

S

T

T

C

F

F

M

N

R

M

L

V

L

N

L

G

A

Q

A

L

E

S

T

P

P

S

T

S

S

G

G

S

D

I

V

R

R

F

V

D

S

G

K

H

F

E

H

L

V

V

N

G

K

M

L

R

R

P

G

R

R

D

H

I

V

W

P

R

K

L

L

G

R

-

Q

-

V

V

I

G

G

R
x
C

T

V

F

F

Q

Q

I

D

A

F

A

R

T

L

F

L

N

Q

S

Q

M

K

T

T

V

V

L

Y

E

D

N

N

V

V

Q

A

M

F

A

A

L

L

V

-

S

-

R

-

E

-

R

-

K

E

T

V

F

I

G

G

V

K

W

R

K

T

P

D

A

A

A

I

S

N

R

R

Y

V

A

V

D

P

E

E

A

-

F

-

A

-

L

V

L

L

E

E

Q

T

V

V

G

G

M

L

A

S

A

G

D

K

A

A

N

N

R

R

A

L

C

P

G

D

V

E

L

L

A

S

Y

G

G

G

D

E

V

Q

K

Q

R

R

V

V

E

A

L

I

A

A

I

R

A

A

L

F

A

V

N

N

R

R

P

P

K

L

L

V

L

L

M

A

D

D

E

E

P

P

T

T

A

G

G

N

M

L

A

D

P

P

K

E

E

T

R

S

N

R

E

D

L

I

M

M

A

D

L

L

T

L

K

E

R

R

L

-

V

I

T

N

E

R

Q

T

K

G

I

T

G

T

V

V

L

L

F

M

T

A

E

T

H

H

S

D

M

H

D

H

V

I

V

V

F

D

A

S

Y

M

A

R

D

Q

R

R

M

V

I

V

V

E

L

L

A

S

R

L

G

G

K

R

L

L

I

V

A

R

E

D

K42 (= K42) mutation to R: Does not bind ATP. Does not affect dimerization.
C84 (≠ R82) mutation to A: Does not affect ATPase activity.

8iddA Cryo-em structure of mycobacterium tuberculosis atp bound ftsex/ripc complex in peptidisc (see paper)
31% identity, 84% coverage: 1:226/269 of query aligns to 3:220/225 of 8iddA

query
sites
8iddA

M

I

S

T

L

L

L

D

H

H

V

V

S

T

G

K

L

Q

C
x
Y

K

K

S

S

F

-

G

S

G

A

L

R

K

P

A

A

V

L

D

D

V

I

S

N

F

V

T

K

L

I

E

D

A

K

G

G

Q

E

L

F

L

V

A

F

L

L

L

I

G

G

P

P

N
x
S

G

G

A

S

G
|
G

K
|
K

S
|
S

T

T

C

F

F

M

N

R

M

L

V

L

N

L

G

A

Q

A

L

E

S

T

P

P

S

T

S

S

G

G

S

D

I

V

R

R

F

V

D

S

G

K

H

F

E

H

L

V

V

N

G

K

M

L

R

R

P

G

R

R

D

H

I

V

W

P

R

K

L

L

G

R

-

Q

-

V

V

I

G

G

R

C

T

V

F

F

Q

Q

I

D

A

F

A

R

T

L

F

L

N

Q

S

Q

M

K

T

T

V

V

L

Y

E

D

N

N

V

V

Q

A

M

F

A

A

L

L

V

-

S

-

R

-

E

-

R

-

K

E

T

V

F

I

G

G

V

K

W

R

K

T

P

D

A

A

A

I

S

N

R

R

Y

V

A

V

D

P

E

E

A

-

F

-

A

-

L

V

L

L

E

E

Q

T

V

V

G

G

M

L

A

S

A

G

D

K

A

A

N

N

R

R

A

L

C

P

G

D

V

E

L

L

A

S

Y

G

G

G

D

E

V

Q

K

Q

R

R

V

V

E

A

L

I

A

A

I

R

A

A

L

F

A

V

N

N

R

R

P

P

K

L

L

V

L

L

M

A

D

D

E

E

P

P

T

T

A

G

G

N

M

L

A

D

P

P

K

E

E

T

R

S

N

R

E

D

L

I

M

M

A

D

L

L

T

L

K

E

R

R

L

-

V

I

T

N

E

R

Q

T

K

G

I

T

G

T

V

V

L

L

F

M

T

A

E

T

H

H

S

D

M

H

D

H

V

I

V

V

F

D

A

S

Y

M

A

R

D

Q

R

R

M

V

I

V

V

E

L

L

A

S

R

L

G

G

K

R

L

L

I

V

A

R

E

D

binding adenosine-5'-triphosphate: Y12 (≠ C10), S39 (≠ N38), G42 (= G41), K43 (= K42), S44 (= S43)

8igqA Cryo-em structure of mycobacterium tuberculosis adp bound ftsex/ripc complex in peptidisc (see paper)
31% identity, 84% coverage: 1:226/269 of query aligns to 3:220/227 of 8igqA

query
sites
8igqA

M

I

S

T

L

L

L

D

H

H

V

V

S

T

G

K

L

Q

C
x
Y

K

K

S

S

F

-

G

S

G

A

L

R

K

P

A

A

V

L

D

D

V

I

S

N

F

V

T

K

L

I

E

D

A

K

G

G

Q

E

L

F

L

V

A

F

L

L

L

I

G

G

P

P

N

S

G
|
G

A
x
S

G

G

K
|
K

S
|
S

T

T

C

F

F

M

N

R

M

L

V

L

N

L

G

A

Q

A

L

E

S

T

P

P

S

T

S

S

G

G

S

D

I

V

R

R

F

V

D

S

G

K

H

F

E

H

L

V

V

N

G

K

M

L

R

R

P

G

R

R

D

H

I

V

W

P

R

K

L

L

G

R

-

Q

-

V

V

I

G

G

R

C

T

V

F

F

Q

Q

I

D

A

F

A

R

T

L

F

L

N

Q

S

Q

M

K

T

T

V

V

L

Y

E

D

N

N

V

V

Q

A

M

F

A

A

L

L

V

-

S

-

R

-

E

-

R

-

K

E

T

V

F

I

G

G

V

K

W

R

K

T

P

D

A

A

A

I

S

N

R

R

Y

V

A

V

D

P

E

E

A

-

F

-

A

-

L

V

L

L

E

E

Q

T

V

V

G

G

M

L

A

S

A

G

D

K

A

A

N

N

R

R

A

L

C

P

G

D

V

E

L

L

A

S

Y

G

G

G

D

E

V

Q

K

Q

R

R

V

V

E

A

L

I

A

A

I

R

A

A

L

F

A

V

N

N

R

R

P

P

K

L

L

V

L

L

M

A

D

D

E

E

P

P

T

T

A

G

G

N

M

L

A

D

P

P

K

E

E

T

R

S

N

R

E

D

L

I

M

M

A

D

L

L

T

L

K

E

R

R

L

-

V

I

T

N

E

R

Q

T

K

G

I

T

G

T

V

V

L

L

F

M

T

A

E

T

H

H

S

D

M

H

D

H

V

I

V

V

F

D

A

S

Y

M

A

R

D

Q

R

R

M

V

I

V

V

E

L

L

A

S

R

L

G

G

K

R

L

L

I

V

A

R

E

D

binding adenosine-5'-diphosphate: Y12 (≠ C10), G40 (= G39), S41 (≠ A40), K43 (= K42), S44 (= S43)

3c4jA Abc protein artp in complex with atp-gamma-s
29% identity, 93% coverage: 1:249/269 of query aligns to 1:242/242 of 3c4jA

query
sites
3c4jA

M

L

S

Q

L

M

L

I

H

D

V

V

S

H

G

Q

L

L

C

K

K

K

S

S

F
|
F

G

G

G

S

L

L

K

E

A
x
V

V

L

D

K

D

G

V

I

S

N

F

V

T

H

L

I

E

R

A

E

G

G

Q

E

L

V

A

V

L

V

L

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

C

F

F

L

N

R

M

C

V

L

N

N

G

L

Q

L

L

E

S

D

P

F

S

D

S

E

G

G

S

E

I

I

R

I

F

I

D

D

G

G

H

-

E

-

L

-

V

I

G

N

M

L

R

K

P

A

R

K

D

D

I

T

-

N

-

L

-

N

-

K

W

V

R

R

L

E

G

E

V

V

G

G

R

M

T

V

F

F

Q

Q

I

R

A

F

A

N

T

L

F

F

N

P

S

H

M

M

T

T

V

V

L

L

E

N

N

N

V

I

Q

T

M

L

A

A

L

-

V

-

S

-

R

-

E

-

R

-

K

-

T

P

F

M

G

K

V

V

W

R

K

K

P

W

A

P

A

R

S

E

R

K

Y

A

A

E

D

A

E

K

A

A

F

M

A

E

L

L

E

D

Q

K

V

V

G

G

M

L

A

K

A

D

D

K

A

A

N

H

R

A

A

Y

C

P

G

D

V

S

L

L

A

S

Y

G

G

G

D

Q

V

A

K

Q

R

R

V

V

E

A

L

I

A

A

I

R

A

A

L

L

A

A

N

M

R

E

P

P

K

K

L

I

L

M

L

L

M

F

D

D

E

E

P

P

T

T

A

S

G

A

M

L

A

D

P

P

K

E

E

M

R

V

N

G

E

E

L

V

M

L

A

S

L

V

T

M

K

K

R

Q

L

L

V

A

T

N

E

E

Q

G

K

M

I

T

G

M

V

V

L

V

F

V

T

T

E

-

H

H

S

E

M

M

D

G

V

F

V

A

F

R

A

E

Y

V

A

G

D

D

R

R

M

V

I

L

V

F

L

M

A

D

R

G

G

G

K

Y

L

I

I

I

A

E

E

E

G

G

D

K

G

P

D

E

T

D

I

L

R

F

N

D

D

R

P

P

R

Q

V

H

Q

E

E

R

V

T

Y

K

F

A

G

F

T

L

G

S

K

K

T

V

F

F

binding phosphothiophosphoric acid-adenylate ester: F13 (= F13), V18 (≠ A18), S38 (≠ N38), G39 (= G39), S40 (≠ A40), G41 (= G41), K42 (= K42), S43 (= S43), T44 (= T44)

3c41J Abc protein artp in complex with amp-pnp/mg2+
29% identity, 93% coverage: 1:249/269 of query aligns to 1:242/242 of 3c41J

query
sites
3c41J

M

L

S

Q

L

M

L

I

H

D

V

V

S

H

G

Q

L

L

C

K

K

K

S

S

F
|
F

G

G

G

S

L

L

K

E

A
x
V

V

L

D

K

D

G

V

I

S

N

F

V

T

H

L

I

E

R

A

E

G

G

Q

E

L

V

A

V

L

V

L

I

G

G

P

P

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

C

F

F

L

N

R

M

C

V

L

N

N

G

L

Q

L

L

E

S

D

P

F

S

D

S

E

G

G

S

E

I

I

R

I

F

I

D

D

G

G

H

-

E

-

L

-

V

I

G

N

M

L

R

K

P

A

R

K

D

D

I

T

-

N

-

L

-

N

-

K

W

V

R

R

L

E

G

E

V

V

G

G

R

M

T

V

F

F

Q

Q

I

R

A

F

A

N

T

L

F

F

N

P

S

H

M

M

T

T

V

V

L

L

E

N

N

N

V

I

Q

T

M

L

A

A

L

-

V

-

S

-

R

-

E

-

R

-

K

-

T

P

F

M

G

K

V

V

W

R

K

K

P

W

A

P

A

R

S

E

R

K

Y

A

A

E

D

A

E

K

A

A

F

M

A

E

L

L

E

D

Q

K

V

V

G

G

M

L

A

K

A

D

D

K

A

A

N

H

R

A

A

Y

C

P

G

D

V

S

L

L

A

S

Y

G

G

G

D

Q

V

A

K

Q

R

R

V

V

E

A

L

I

A

A

I

R

A

A

L

L

A

A

N

M

R

E

P

P

K

K

L

I

L

M

L

L

M

F

D
|
D

E

E

P

P

T

T

A

S

G

A

M

L

A

D

P

P

K

E

E

M

R

V

N

G

E

E

L

V

M

L

A

S

L

V

T

M

K

K

R

Q

L

L

V

A

T

N

E

E

Q

G

K

M

I

T

G

M

V

V

L

V

F

V

T

T

E

-

H

H

S

E

M

M

D

G

V

F

V

A

F

R

A

E

Y

V

A

G

D

D

R

R

M

V

I

L

V

F

L

M

A

D

R

G

G

G

K

Y

L

I

I

I

A

E

E

E

G

G

D

K

G

P

D

E

T

D

I

L

R

F

N

D

D

R

P

P

R

Q

V

H

Q

E

E

R

V

T

Y

K

F

A

G

F

T

L

G

S

K

K

T

V

F

F

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F13), V18 (≠ A18), S38 (≠ N38), G39 (= G39), G41 (= G41), K42 (= K42), S43 (= S43), T44 (= T44)
binding magnesium ion: S43 (= S43), D163 (= D169)

2olkA Abc protein artp in complex with adp-beta-s
29% identity, 93% coverage: 1:249/269 of query aligns to 1:242/242 of 2olkA

query
sites
2olkA

M

L

S

Q

L

M

L

I

H

D

V

V

S

H

G

Q

L

L

C

K

K

K

S

S

F
|
F

G

G

G

S

L

L

K

E

A
x
V

V

L

D

K

D

G

V

I

S

N

F

V

T

H

L

I

E

R

A

E

G

G

Q

E

L

V

A

V

L

V

L

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

C

F

F

L

N

R

M

C

V

L

N

N

G

L

Q

L

L

E

S

D

P

F

S

D

S

E

G

G

S

E

I

I

R

I

F

I

D

D

G

G

H

-

E

-

L

-

V

I

G

N

M

L

R

K

P

A

R

K

D

D

I

T

-

N

-

L

-

N

-

K

W

V

R

R

L

E

G

E

V

V

G

G

R

M

T

V

F

F

Q

Q

I

R

A

F

A

N

T

L

F

F

N

P

S

H

M

M

T

T

V

V

L

L

E

N

N

N

V

I

Q

T

M

L

A

A

L

-

V

-

S

-

R

-

E

-

R

-

K

-

T

P

F

M

G

K

V

V

W

R

K

K

P

W

A

P

A

R

S

E

R

K

Y

A

A

E

D

A

E

K

A

A

F

M

A

E

L

L

E

D

Q

K

V

V

G

G

M

L

A

K

A

D

D

K

A

A

N

H

R

A

A

Y

C

P

G

D

V

S

L

L

A

S

Y

G

G

G

D

Q

V

A

K

Q

R

R

V

V

E

A

L

I

A

A

I

R

A

A

L

L

A

A

N

M

R

E

P

P

K

K

L

I

L

M

L

L

M

F

D

D

E

E

P

P

T

T

A

S

G

A

M

L

A

D

P

P

K

E

E

M

R

V

N

G

E

E

L

V

M

L

A

S

L

V

T

M

K

K

R

Q

L

L

V

A

T

N

E

E

Q

G

K

M

I

T

G

M

V

V

L

V

F

V

T

T

E

-

H

H

S

E

M

M

D

G

V

F

V

A

F

R

A

E

Y

V

A

G

D

D

R

R

M

V

I

L

V

F

L

M

A

D

R

G

G

G

K

Y

L

I

I

I

A

E

E

E

G

G

D

K

G

P

D

E

T

D

I

L

R

F

N

D

D

R

P

P

R

Q

V

H

Q

E

E

R

V

T

Y

K

F

A

G

F

T

L

G

S

K

K

T

V

F

F

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (= F13), V18 (≠ A18), S38 (≠ N38), G39 (= G39), S40 (≠ A40), G41 (= G41), K42 (= K42), S43 (= S43), T44 (= T44)

2oljA Abc protein artp in complex with adp/mg2+
29% identity, 93% coverage: 1:249/269 of query aligns to 1:242/242 of 2oljA

query
sites
2oljA

M

L

S

Q

L

M

L

I

H

D

V

V

S

H

G

Q

L

L

C

K

K

K

S

S

F
|
F

G

G

G

S

L

L

K

E

A
x
V

V

L

D

K

D

G

V

I

S

N

F

V

T

H

L

I

E

R

A

E

G

G

Q

E

L

V

A

V

L

V

L

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

C

F

F

L

N

R

M

C

V

L

N

N

G

L

Q

L

L

E

S

D

P

F

S

D

S

E

G

G

S

E

I

I

R

I

F

I

D

D

G

G

H

-

E

-

L

-

V

I

G

N

M

L

R

K

P

A

R

K

D

D

I

T

-

N

-

L

-

N

-

K

W

V

R

R

L

E

G

E

V

V

G

G

R

M

T

V

F

F

Q

Q

I

R

A

F

A

N

T

L

F

F

N

P

S

H

M

M

T

T

V

V

L

L

E

N

N

N

V

I

Q

T

M

L

A

A

L

-

V

-

S

-

R

-

E

-

R

-

K

-

T

P

F

M

G

K

V

V

W

R

K

K

P

W

A

P

A

R

S

E

R

K

Y

A

A

E

D

A

E

K

A

A

F

M

A

E

L

L

E

D

Q

K

V

V

G

G

M

L

A

K

A

D

D

K

A

A

N

H

R

A

A

Y

C

P

G

D

V

S

L

L

A

S

Y

G

G

G

D

Q

V

A

K

Q

R

R

V

V

E

A

L

I

A

A

I

R

A

A

L

L

A

A

N

M

R

E

P

P

K

K

L

I

L

M

L

L

M

F

D

D

E

E

P

P

T

T

A

S

G

A

M

L

A

D

P

P

K

E

E

M

R

V

N

G

E

E

L

V

M

L

A

S

L

V

T

M

K

K

R

Q

L

L

V

A

T

N

E

E

Q

G

K

M

I

T

G

M

V

V

L

V

F

V

T

T

E

-

H

H

S

E

M

M

D

G

V

F

V

A

F

R

A

E

Y

V

A

G

D

D

R

R

M

V

I

L

V

F

L

M

A

D

R

G

G

G

K

Y

L

I

I

I

A

E

E

E

G

G

D

K

G

P

D

E

T

D

I

L

R

F

N

D

D

R

P

P

R

Q

V

H

Q

E

E

R

V

T

Y

K

F

A

G

F

T

L

G

S

K

K

T

V

F

F

binding adenosine-5'-diphosphate: F13 (= F13), V18 (≠ A18), S38 (≠ N38), G39 (= G39), S40 (≠ A40), G41 (= G41), K42 (= K42), S43 (= S43), T44 (= T44)

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
31% identity, 87% coverage: 3:237/269 of query aligns to 17:240/378 of P69874

query
sites
P69874

L

L

L

V

H

Q

V

L

S

A

G

G

L

I

C

R

K

K

S
x
C

F
|
F

G

D

G

G

L

K

K

E

A

V

V

I

D

P

D

Q

V

L

S

D

F

L

T

T

L

I

E

N

A

N

G

G

Q

E

L
x
F

L

L

A

T

L

L

G

G

P

P

N

S

G

G

A
x
C

G

G

K

K

S

T

T

T

C

V

F
x
L

N

R

M

L

V

I

N

A

G

G

Q

L

L

E

S

T

P

V

S

D

S

S

G

G

S

R

I

I

R

M

F
x
L

D

D

G

N

H

E

E

D

L

I

V

T

G

H

M

V

R

P

P

A

R

E

D

N

I

R

W

Y

R

-

L

-

G

-

V

V

G

N

R

T

T

V

F

F

Q

Q

I

S

A

Y

A

A

T

L

F

F

N

P

S

H

M

M

T

T

V

V

L

F

E

E

N

N

V

V

Q

A

M

F

A

G

L

L

V

-

S

-

R

R

E

M

R

Q

K

K

T

T

F

-

G

-

V

-

W

-

K

-

P

P

A

A

S

E

R

-

Y

I

A

T

D

P

E

R

A

V

F

M

A

E

L

A

L

L

E

R

Q

M

V
|
V

G

Q

M

L

A

E

A

T

D

F

A

A

N

Q

R

R

A

K

C

P

G

H

V

Q

L

L

A

S

Y

G

G

G

D

Q

V

Q

K

Q

R

R

V

V

E

A

L

I

A

A

I

R

A

A

L

V

A

V

N

N

R

K

P

P

K

R

L

L

M

L

D
|
D

E

E

P

S

T

L

A

S

G

A

M

L

A

D

P

Y

K

K

E

L

R

R

-

K

-

Q

-

M

-

Q

N

N

E

E

L

L

M

K

A

A

L

L

T

Q

K

R

R

K

L

L

V

-

T

-

E

-

Q

-

K

G

I

I

G

T

V

F

L

V

F

F

T

V

E

T

H

H

S

D

M

Q

D

E

V

E

V

A

F

L

A

T

Y

M

A

S

D

D

R

R

M

I

I

V

V

V

L

M

A

R

R

D

G

G

K

R

L

I

I

E

A

Q

E

D

G

G

D

T

G

P

D

R

T

E

I

I

R

Y

N

E

D

E

P

P

R

K

C26 (≠ S12) mutation to A: Lower ATPase activity and transport efficiency.
F27 (= F13) mutation to L: Lower ATPase activity and transport efficiency.
F45 (≠ L31) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ A40) mutation to T: Loss of ATPase activity and transport.
L60 (≠ F46) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ F62) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V132) mutation to M: Loss of ATPase activity and transport.
D172 (= D169) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

Query Sequence

>H281DRAFT_01586 H281DRAFT_01586 amino acid/amide ABC transporter ATP-binding protein 1, HAAT family
MSLLHVSGLCKSFGGLKAVDDVSFTLEAGQLLALLGPNGAGKSTCFNMVNGQLSPSSGSI
RFDGHELVGMRPRDIWRLGVGRTFQIAATFNSMTVLENVQMALVSRERKTFGVWKPAASR
YADEAFALLEQVGMAADANRACGVLAYGDVKRVELAIALANRPKLLLMDEPTAGMAPKER
NELMALTKRLVTEQKIGVLFTEHSMDVVFAYADRMIVLARGKLIAEGDGDTIRNDPRVQE
VYFGTGKTFQPHAALHDMAAGHAARGALQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory