SitesBLAST

D57 (= D38) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (= R92) mutation to A: Loss of transport activity.

Sites not aligning to the query:

54 D→A: Loss of transport activity.; D→E: Retains 50% of its transport activity.
309 W→V: Loss of transport activity.
312 D→A: Loss of transport activity.
313 R→A: Loss of transport activity.
317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

O15245 Solute carrier family 22 member 1; Organic cation transporter 1; hOCT1 from Homo sapiens (Human) (see 10 papers)
25% identity, 81% coverage: 82:433/435 of query aligns to 162:517/554 of O15245

query
sites
O15245

G

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S189 (≠ A109) to L: no changes in MPP(+) uptake; dbSNP:rs34104736
G220 (= G148) to V: affects transporter activity; reduction of MPP(+) uptake when associated with V-408; dbSNP:rs36103319
Y240 (≠ S168) mutation to F: Decreased TEA uptake.
P283 (≠ G213) to L: in dbSNP:rs4646277; mutation to A: Decreased TEA uptake.
R287 (= R217) to G: in dbSNP:rs4646278
P341 (≠ M255) to L: affects transporter activity; reduction of TEA uptake; reduction o MPP(+) uptake when associated with V-408; largely localized to the plasma membrane; dbSNP:rs2282143
R342 (≠ G256) to H: no changes in MPP(+) uptake when associated with V-408; dbSNP:rs34205214
Y361 (= Y275) mutation to F: Decreased TEA uptake.
Y376 (≠ L287) mutation to F: Decreased TEA uptake.
G401 (= G312) to S: affects transporter activity; reduction of MPP(+), serotonin and TEA uptake; no MPP(+) uptake when associated with L-160; dbSNP:rs34130495
M408 (≠ A321) to V: does not affect transporter activity; no changes in MPP(+) uptake when associated with F-14; no changes in MPP(+) uptake when associated with F-85; no changes in MPP(+) uptake when associated with L-189; no changes in MPP(+) uptake when associated with H-342; no changes in MPP(+) uptake when associated with M-420 del; no changes in MPP(+) uptake when associated with I-440; no changes in MPP(+) uptake when associated with I-461; no changes in MPP(+) uptake when associated with M-488; reduction of MPP uptake when associated with C-61; no MPP(+) uptake when associated with V-220; reduction of MPP(+) uptake when associated with L-341; no MPP(+) uptake when associated with S-401; no MPP(+) uptake when associated with R-465; dbSNP:rs628031
M420 (≠ W333) natural variant: Missing (reduction of serum O-isobutanoyl-(R)-carnitine levels; no change in MPP(+) uptake; no changes in MPP(+) uptake when associated with V-408; dbSNP:rs72552763)
M440 (= M358) to I: in dbSNP:rs35956182
V461 (= V374) to I: no changes in MPP(+) uptake when associated with V-408; dbSNP:rs34295611
G465 (= G378) to R: reduction of the localization to the basolateral membrane; no MPP(+) uptake when associated with V-408; dbSNP:rs34059508; mutation to A: No changes in MPP(+) uptake.
R488 (≠ L402) to M: no changes in MPP(+) uptake when associated with V-408; dbSNP:rs35270274

Sites not aligning to the query:

14 S → F: exclusively found in the African American population; increased MPP(+) uptake when associated with V-408; dbSNP:rs34447885
24 I→L: No change in fenoterol uptake. No change in trospium uptake. No change in terbutaline uptake.
28 L→I: No change in fenoterol uptake. No change in trospium uptake. No change in terbutaline uptake.
31 A→S: No change in fenoterol uptake. No change in trospium uptake. No change in terbutaline uptake.
32 F→L: No change in fenoterol uptake. Decreased trospium uptake. Decreased trospium affinity.
36 C→Y: Increased fenoterol uptake. Increased fenoterol affinity. No change in trospium uptake. No change in terbutaline uptake. No change in terbutaline affinity.
41 F → L: in dbSNP:rs2297373
61 R → C: affects transporter activity; reduction of MPP(+) uptake; reduction of serum O-isobutanoyl-(R)-carnitine levels; reduction of MPP(+) uptake when associated with V-408; dbSNP:rs12208357
85 L → F: no changes in MPP(+) uptake; when associated with V-408; dbSNP:rs35546288
88 C → R: affects transporter activity; reduction of MPP(+), serotonin and TEA uptake; dbSNP:rs55918055
160 L → F: no changes in both MPP(+) and TEA uptake; abolishes MPP(+) uptake when associated with S-401; largely localized to the plasma membrane; dbSNP:rs683369

8et8A Cryo-em structure of the organic cation transporter 1 in complex with verapamil (see paper)
24% identity, 77% coverage: 81:417/435 of query aligns to 160:501/532 of 8et8A

query
sites
8et8A

L

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binding (2S)-2-(3,4-dimethoxyphenyl)-5-{[2-(3,4-dimethoxyphenyl)ethyl](methyl)amino}-2-(propan-2-yl)pentanenitrile: K213 (≠ G142), W216 (= W145), Q240 (= Q169), W353 (≠ G280), Y360 (≠ L287), F378 (vs. gap), S381 (vs. gap), E385 (= E297), C449 (≠ A364), S469 (≠ H383)

Sites not aligning to the query:

binding (2S)-2-(3,4-dimethoxyphenyl)-5-{[2-(3,4-dimethoxyphenyl)ethyl](methyl)amino}-2-(propan-2-yl)pentanenitrile: 31, 35

8et7A Cryo-em structure of the organic cation transporter 1 in complex with diphenhydramine (see paper)
24% identity, 77% coverage: 81:417/435 of query aligns to 160:501/532 of 8et7A

query
sites
8et7A

L

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G

V

V

A

M

L

V

G

C

H

S

E

S

V

L

A

C

S

D

I

L

F

-

A

G

G

G

G

I

L

I

S

T

P

P

L

F

I

L

A

V

T

F

A

R

L

L

L

M

A

E

H

V

Y

W

H

Q

A

G

Y

L

W

-

P

P

V

L

A

I

V

L

F

F

L

T

I

V

V

V

binding n-[2-(benzhydryloxy)ethyl]-n,n-dimethylamine: E385 (= E297), F445 (≠ G360)

Sites not aligning to the query:

binding n-[2-(benzhydryloxy)ethyl]-n,n-dimethylamine: 35

O15244 Solute carrier family 22 member 2; Organic cation transporter 2; hOCT2 from Homo sapiens (Human) (see 8 papers)
26% identity, 74% coverage: 81:401/435 of query aligns to 162:488/555 of O15244

query
sites
O15244

L

I

G

G

G

S

I
x
M

V

S

F

I

G

G

H
x
Y

I

I

G

A

D

D

R

R

Y

F

G

G

R

R

R

K

A

L

S

C

L

L

V

L

W

T

T

T

L

V

L

L

I

I

M

N

G

A

F

A

A

A

T

G

F

V

G

L

I

M

G

A

L

I

L

S

P

P

T

T

Y

Y

D
x
T

H

-

V

-

G

-

L

-

W

W

A

-

P

-

A

-

L

M

V

L

A

I

L

F

R

R

L

L

L

I

Q

Q

G

G

I

L

A

V

S

S

G

K

G

A

E

G

W

W

G

L

G

I

G

G

V

Y

L

I

M

L

I

I

S

T

E

E

S

F

A

V

P

G

P

R

E

R

K

Y

R

R

G

R

Y

T

Y

V

A

G

A

I

W

F

S
x
Y

Q

Q

L

V

G

A

V

Y

G

T

G

V

G

G

-

L

F

L

V

V

L

L

S

A

S

G

A

V

A

A

F
x
Y

L

-

G

-

V

-

Q

-

A

A

L

L

P

P

H

H

D

-

Q

-

F

-

V

-

S

-

W

W

G

R

W

W

R

-

L

L

P

Q

F

F

L

T

A

V

S
|
S

I

L

L

P

I

N

F

F

A

F

L

F

G

L

V

L

Y
|
Y

I
x
Y

R

-

H

W

N

C

L

I

P
|
P

E
|
E

S
|
S

R
x
P

D
x
R

F

W

E

L

N

I

A

S

D

Q

K

N

A

K

G

N

K

A

T

E

A

A

H

M

M

R

P

I

V

I

V

K

E

H

V

I

I

A

K

K

R

K

H

N

P

G

K

K

E

S

I

L

L

P

M

A

A

S

M

L

G

Q

-

R

L

L

R

R

V

L

A

E

E

E

N

E

G

T

G

G

A

K

Y

K

I

L

F

N

L

P

A

S

F

F

-

L

S

D

L

L

V

V

Y

R

G

T

K

P

-

Q

-

I

-

R

-

K

-

H

-

T

-

M

-

I

-

L

-

M

Y

Y

A

N

G

W

I

F

P

T

S

S

T

V

M

L

L
x
Y

T

Q

G

G

V

L

M

I

L

M

A

H

M

M

-

G

-

L

-

A

-

G

-

D

-

N

-

I

-
x
Y

-

L

-

D

-

F

-

Y

-

S

-

A

V

L

V

V

E

E

M

F

V

P

A

A

M

A

L

F

L

M

W

I

G

I

K

L

L

T

S

I

D

D

R
|
R

I

I

G

G

R

R

K

R

P

-

V

-

Y

Y

M

P

I

W

G

A

A

A

I

S

S

N

L

M

V

V

A

A

M

G

A

A

F

A

P

-

F

-

W

-

L

C

L

L

N

A

T

S

H

V

S

F

T

I

P

P

-

G

-

D

L

L

I

Q

W

W

L

L

A
x
K

L

I

V

I

L

I

G

S

T

-

A

-

V

-

C

C

H

L

G

G

A

R

M

M

I

G

G

I

T

T

L

M

P

A

-

Y

-

E

-

I

-

V

A

C

L

L

V

V

G

N

-

A

E

E

L

L

F
x
Y

S

P

T

T

E

F

V

I

R

R

Y

N

S

L

G

G

V

V

A

H

L

I

G

C

H

S

E

S

V

M

A

C

S

D

I

I

F

-

A

G

G

G

G

I

L

I

S

T

P

P

L

F

I

L

A

V

T

Y

A

R

L

L

M165 (≠ I84) to I: lower Vmax for MPP(+) transport; no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs8177507
Y169 (≠ H88) mutation to F: No change in TEA uptake.
T201 (≠ D120) to M: in dbSNP:rs145450955
Y241 (≠ S168) mutation to F: Slight decrease in TEA uptake. No change in tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-362. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-362 and F-377.
Y257 (≠ F183) mutation to F: No change in TEA uptake.
S270 (= S206) to A: decreased Ki value for TBA inhibition of MPP(+); no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs316019
Y279 (= Y215) mutation to F: No change in TEA uptake.
Y280 (≠ I216) mutation to F: No change in TEA uptake.
P284 (= P221) mutation to A: Decreased TEA and metformin uptake. Decreased tyrosine phosphorylation.
PESPR 284:288 (≠ PESRD 221:225) Proline-rich sequence
S286 (= S223) mutation to A: No change in TEA and metformin uptake. No change in tyrosine phosphorylation.
P287 (≠ R224) mutation to A: Decreased TEA and metformin uptake. Decreased tyrosine phosphorylation.
Y362 (≠ L287) mutation to F: Decreased TEA uptake and YES1-mediated tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-241. Strong decrease in TEA uptake; when associated with F-377. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-241 and F-377.
Y377 (vs. gap) mutation to F: Slight decrease in TEA uptake. No change in tyrosine phosphorylation. Strong decrease in TEA uptake; when associated with F-362. Strong decrease in TEA and metformin uptake and YES1-mediated tyrosine phosphorylation; when associated with F-241 and F-362.
R400 (= R310) to C: lower Vmax and reduced Ki value for TBA inhibition of MPP(+); lower transport efficiency (Vmax/Km) and clearance of cyclo(his-pro); no change in transport efficiency (Vmax/Km) and clearance of salsolinol; dbSNP:rs8177516
K432 (≠ A346) to Q: lower Km value for MPP(+) and reduced Ki value for TBA inhibition of MPP; no change in transport efficiency (Vmax/Km) and clearance of cyclo(his-pro) and salsolinol; dbSNP:rs8177517
Y458 (≠ F370) mutation to F: No change in TEA uptake.

Sites not aligning to the query:

54 P → S: in dbSNP:rs8177504
73 Y→F: No change in TEA uptake.
92 Y→F: No change in TEA uptake.
128 Y→F: No change in TEA uptake.
544 Y→F: No change in TEA uptake.

O08966 Solute carrier family 22 member 1; Organic cation transporter 1; mOCT1 from Mus musculus (Mouse) (see paper)
25% identity, 75% coverage: 81:407/435 of query aligns to 162:494/556 of O08966

query
sites
O08966

L

L

G

G

G

S

I

L

V

V

F

V

G

G

H

Y

I

I

G

A

D

D

R

R

Y

F

G

G

R

R

R

K

A

L

S

C

L

L

V

L

W

V

T

T

L

T

L

L

I

V

M

T

G

S

F

L

A

S

T

G

F

V

G

L

I

T

G

A

L

V

L

A

P

P

T

D

Y

Y

D

-

H

-

V

-

G

-

L

-

W

-

A

-

P

-

A

T

A

S

L

M

V

L

A

L

L

F

R

R

L

L

Q

Q

G

G

I

M

A

V

S

S

G

K

G

G

E

S

W

W

G

V

G

S

G

G

V

Y

L

T

M

L

I

I

S

T

E

E

S

F

A

V

P

G

P

S

E

G

K

Y

R

R

G

R

Y

T

A

A

A

I

W

L

S

Y

Q

Q

L

V

G

A

V

F

G

T

G

V

G

G

F

L

V

V

-

G

L

L

S

A

S

G

A

V

A

A

F

Y

-

A

-

I

-

P

-

D

-

W

-

R

-

W

-

L

-

Q

L

L

G

A

V

V

Q

-

A

S

L

L

P

P

H

T

D

F

Q

L

F

F

V

L

S

L

W

Y

G

Y

W

W

R

F

L

V

P

P

-

E

-

S

-

P

-

R

F

W

L

L

A

L

S

S

I

Q

L

K

I

R

F

T

A

T

L

Q

G

A

V

V

Y

R

I

I

R

M

H

E

N

Q

L

I

P

A

E

Q

-

K

S

N

R

R

D

K

F

V

E

P

N

P

A

A

D

D

K

L

A

K

G

M

K

M

T

C

A

L

H

E

M

E

P

D

V

A

V

S

E

E

V

-

I

-

K

R

R

R

H

S

P

P

K

S

-

F

-

A

E

D

I

L

L

F

M

R

A

T

M

P

G

S

L

L

R

R

V

K

A

H

E

T

N

L

G

I

G

L

A

M

Y

Y

I

L

F

W

L

F

A

S

F

C

S

A

L

V

V

L

Y

Y

G

Q

-

G

-

L

-

I

K

M

Y

H

A

V

G

G

I

A

P

T

S

G

S

A

T

N

M

L

L

Y

T

L

G

D

V

F

M

F

L

Y

A

S

M

S

V

L

V

V

E

E

M

F

V

P

A

A

M

A

L

F

L

I

W

I

G

L

K

V

L

T

S

I

D

D

R

R

I

I

G

G

R

R

K

-

P

-

V

I

Y

Y

M

P

I

I

G

A

A

A

I

S

S

N

L

L

V

V

A

A

M

G

A

A

F

A

P

C

F

L

W

M

L

I

L

F

N

I

T

P

H

H

S

E

T

-

P

-

L

L

I

H

W

W

L

L

A

N

L

V

V

T

L

L

G

A

T

-

A

-

V

-

C

C

H

L

G

G

A

R

M

M

I

G

G

A

T

T

-

I

-

V

-

L

-

Q

L

M

P

V

A

C

L

L

V

V

G

N

-

A

E

E

L

L

F

Y

S

P

T

T

E

F

V

I

R

R

Y

N

S

L

G

G

V

M

A

M

L

V

G

C

H

S

E

A

V

L

A

C

S

D

I

L

F

-

A

G

G

G

G

I

L

F

S

T

P

P

L

F

I

M

A

V

T

F

A

R

L

L

L

M

A

E

H

V

Y

W

H

Q

A

A

Sites not aligning to the query:

32 L→F: Increased trospium uptake. Increased trospium affinity. No change in fenoterol uptake.
36 Y→C: Decreased fenoterol uptake. Decreased fenoterol affinity. No change in trospium uptake. No change in terbutaline affinity.

Q63089 Solute carrier family 22 member 1; Organic cation transporter 1; rOCT1 from Rattus norvegicus (Rat) (see 4 papers)
25% identity, 77% coverage: 81:414/435 of query aligns to 162:500/556 of Q63089

query
sites
Q63089

L

L

G

G

G

S

I

L

V

V

F

V

G

G

H

Y

I

I

G

A

D

D

R

R

Y

F

G

G

R

R

R

K

A

L

S
x
C

L

L

V

L

W

V

T

T

L

T

L

L

I

V

M

T

G

S

F

V

A

S

T

G

F

V

G

L

I

T

G

A

L

V

L

A

P

P

T

D

Y

Y

D

-

H

-

V

-

G

-

L

-

W

-

A

-

P

-

A

T

A

S

L

M

V

L

A

L

L

F

R

R

L

L

Q

Q

G

G

I
x
M

A
x
V

S
|
S

G
x
K

G
|
G

E
x
S

W
|
W

G
x
V

G
x
S

G
|
G

V
x
Y

L
x
T

M
x
L

I
|
I

S
x
T

E
|
E

S
x
F

A
x
V

P

G

P

S

E

G

K

Y

R

R

G

R

Y

T

A

A

A

I

W

L

S

Y

Q

Q

L

M

G

A

V

F

G

T

G

V

G

G

F

L

V

V

-

G

L

L

S

A

S

G

A

V

A

A

F

Y

L

-

G

-

V

-

Q

-

A

A

L

I

P

P

H

-

D

-

Q

-

F

-

V

-

S

D

W

W

G

R

W

W

R

-

L

L

P

Q

F

L

L

A

A

V

S

S

I

L

L

P

I

T

F

F

A

L

L

F

G

L

V

L

Y

Y

I

Y

R

W

H

F

N

-

L

V

P

P

E

E

S
|
S

R

P

D

R

F

W

E

L

N

L

A
x
S

D

Q

K

K

-

R

-
x
T

-

T

-

R

-

A

-

V

-

R

-

I

-

M

-

E

-

Q

-

I

A

A

G

Q

K

K

T

N

A

G

H

K

M

V

P

P

V

P

V

A

E

D

V

L

I

K

-

M

-

L

-
x
C

-

L

-

E

-

D

-

A

-
x
S

-

E

K

K

R

R

H

S

P

P

K

S

-

F

-

A

E

D

I

L

L

F

M

R

A

T

M

P

G

N

L

L

R

R

V

K

A

H

E

T

N

V

G

I

G

L

A

M

Y

Y

I

L

F

W

L

F

A

S

F
x
C

S

A

L

V

V

L

Y

Y

G

Q

-

G

-

L

-

I

K

M

Y

H

A

V

G

G

I

A

P

T

S

G

S

A

T

N

M

L

L

Y

T

L

G

D

V

F

M

F

L

Y

A

S

M

S

V

L

V

V

E

E

M

F

V

P

A

A

M

A

L

F

L

I

W

I

G

L

K

V

L

T

S

I

D

D

R

R

I

I

G

G

R

R

K

-

P

-

V

I

Y

Y

M

P

I

I

G

A

A

A

I

S

S

N

L

L

V

V

A

T

M

G

A

A

F

A

P
x
C

F

L

W

M

L

I

L

F

N

I

T

P

H

H

S

E

T

-

P

-

L

L

I

H

W

W

L

L

A

N

L

V

V

T

L

L

G

A

T

-

A

-

V

-

C
|
C

H

L

G

G

A

R

M

M

I

G

G

A

T

T

-

I

-

V

-

L

-

Q

L

M

P

V

A
x
C

L

L

V

V

G

N

-

A

E

E

L

L

F

Y

S

P

T

T

E

F

V

I

R

R

Y

N

S

L

G

G

V

M

A

M

L

V

G
x
C

H

S

E

A

V

L

A
x
C

S
x
D

I

L

F

-

A

G

G

G

G

I

L

F

S

T

P

P

L

F

I

M

A

V

T

F

A

R

L

L

L

M

A

E

H

V

Y

W

H

Q

A

A

Y

L

W

-

P

P

V

L

A

I

V

L

F

F

C179 (≠ S98) mutation to A: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; S-322; A-358; A-418; S-437; A-470 and A-474.
M212 (≠ I139) mutation to L: No change in TEA and MPP(+) uptake.
V213 (≠ A140) mutation to G: Decreased TEA uptake. No change in MPP(+) uptake.
S214 (= S141) mutation to G: Decreased TEA and MPP(+) uptake.
K215 (≠ G142) mutation to Q: Loss of TEA and MPP(+) uptake activity.; mutation to R: Loss of TEA and MPP(+) uptake activity.
G216 (= G143) mutation to A: Decreased TEA and MPP(+) uptake.
S217 (≠ E144) mutation to G: No change in TEA and MPP(+) uptake.
W218 (= W145) mutation to F: Decreased guanidine, histamine, serotonin and TEA uptake. No change in MPP(+) uptake. No change in TEA and MPP(+) affinity. Decreased TEA Vmax. No change in MPP(+) Vmax.; mutation to L: Decreased guanidine, histamine, serotonin, TEA and MPP(+) uptake. Decreased TEA affinity. No change in MPP(+) affinity. Decreased TEA and MPP(+) Vmax.; mutation to Y: Decreased guanidine, histamine, serotonin and TEA uptake. No change in MPP(+) uptake. Increased TEA and MPP(+) affinity. Decreased TEA and MPP(+) Vmax.
V219 (≠ G146) mutation to L: No change in TEA and MPP(+) uptake.
S220 (≠ G147) mutation to I: Decreased TEA and MPP(+) uptake.
G221 (= G148) mutation to A: Decreased TEA and MPP(+) uptake.
Y222 (≠ V149) mutation to F: No change in guanidine, histamine, serotonin, TEA and MPP(+) uptake. Increased TEA affinity. No change in MPP(+) affinity. Decreased TEA Vmax. No change in MPP(+) Vmax.; mutation to L: Decreased guanidine, serotonin, TEA and MPP(+) uptake. No change in histamine uptake. Increased TEA and MPP(+) affinity. Decreased TEA and MPP(+) Vmax.
T223 (≠ L150) mutation to I: Decreased TEA uptake. No change in MPP(+) uptake.
L224 (≠ M151) mutation to V: Decreased TEA and MPP(+) uptake.
I225 (= I152) mutation to G: No change in TEA and MPP(+) uptake.
T226 (≠ S153) mutation to A: Decreased TEA uptake. No change in MPP(+) uptake.
E227 (= E154) mutation to D: Loss of TEA and MPP(+) uptake activity.; mutation to Q: Loss of TEA and MPP(+) uptake activity.
F228 (≠ S155) mutation to I: No change in TEA and MPP(+) uptake.
V229 (≠ A156) mutation to A: Decreased TEA and MPP(+) uptake.; mutation to L: Loss of TEA and MPP(+) uptake activity.
S286 (= S223) mutation to A: No effect of PKC-induced stimulation on ASP uptake. No effect of PKC-induced stimulation on ASP uptake; when associated with A-292; A-296; A-328 and A-550. No effect of PKA activation on ASP uptake. No effect of PKA activation on ASP uptake; when associated with A-292; A-296; A-328 and A-550. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition; when associated with A-292; A-296; A-328 and A-550. No significant effect on trafficking from intracellular pools to the cell membrane; when associated with A-292; A-296; A-328 and A-550. suppresses phosphorylation by PKC; when associated with A-292; A-296; A-328 and A-550.
S292 (≠ A229) mutation to A: No effect of PKC-induced stimulation on ASP uptake. No effect of PKC-induced stimulation on ASP uptake; when associated with A-286; A-296; A-328 and A-550. No effect of PKA activation on ASP uptake. No effect of PKA activation on ASP uptake; when associated with A-286; A-296; A-328 and A-550. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition; when associated with A-286; A-296; A-328 and A-550. No significant effect on trafficking from intracellular pools to the cell membrane; when associated with A-286; A-296; A-328 and A-550. suppresses phosphorylation by PKC; when associated with A-286; A-296; A-328 and A-550.
T296 (vs. gap) mutation to A: No effect of PKC-induced stimulation on ASP uptake. No effect of PKC-induced stimulation on ASP uptake; when associated with A-286; A-292; A-328; A-550. Significant increase of the ASP uptake by PKA activation. No effect of PKA activation on ASP uptake; when associated with A-286; A-292; A-328; A-550. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition; when associated with A-286; A-292; A-328; A-550. No significant effect on trafficking from intracellular pools to the cell membrane; when associated with A-286; A-292; A-328 and A-550. suppresses phosphorylation by PKC; when associated with A-286; A-292; A-328 and A-550.
C322 (vs. gap) mutation to S: Reduces the activation by MMTS. Abolishes the activation by MMTs; when associated with M-451. Choline affinity is increased fivefold by MMTS. Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; A-179; A-358; A-418; S-437; A-470 and A-474. Choline affinity is increased four- to fivefold; when associated with M-451.
S328 (vs. gap) mutation to A: No effect of PKC-induced stimulation on ASP uptake. No effect of PKC-induced stimulation on ASP uptake; when associated with A-286; A-292; A-296 and A-550. No effect of PKA activation on ASP uptake. No effect of PKA activation on ASP uptake; when associated with A-286; A-292; A-296 and A-550. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition; when associated with A-286; A-292; A-296; A-550. No significant effect on trafficking from intracellular pools to the cell membrane; when associated with A-286; A-292; A-296 and A-550. suppresses phosphorylation by PKC; when associated with A-286; A-292; A-296 and A-550.
C358 (≠ F271) mutation to A: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; A-179; S-322; A-418; S-437; A-470 and A-474.
C418 (≠ P330) mutation to A: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; A-179; S-322; A-358; S-437; A-470 and A-474.
C437 (= C354) mutation to S: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; A-179; S-322; A-358; A-418; A-470 and A-474.
C451 (≠ A364) mutation to M: Reduces the activation by MMTS. Abolishes the activation by MMTs; when associated with S-322. Abolishes the effect of MMTs on choline-induced currents. Choline affinity is not influenced by MMTS. Choline affinity is increased four- to fivefold; when associated with S-322.
C470 (≠ G382) mutation to A: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; A-179; S-322; A-358; A-418; A-437 and A-474.
C474 (≠ A386) mutation to A: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-155; A-179; S-322; A-358; A-418; A-437 and A-470.
D475 (≠ S387) mutation to E: Decreased MPP(+) uptake, no change in MPP(+) affinity. Decreased NMN uptake, increased NMN affinity. Decreased choline uptake, increased choline affinity.; mutation to N: Decreased MPP(+) uptake.; mutation to R: Decreased MPP(+) uptake.

Sites not aligning to the query:

26 C→A: Choline affinity is increased fourfold by MMTS; when associated with A-155; A-179; S-322; A-358; A-418; S-437; A-470 and A-474.
155 C→A: Choline affinity is increased fourfold by MMTS; when associated with A-26; A-179; S-322; A-358; A-418; S-437; A-470 and A-474.
550 T→A: No effect of PKC-induced stimulation on ASP uptake. No effect of PKC-induced stimulation on ASP uptake; when associated with A-286; A-292; A-296; A-328. Significant increase of the ASP uptake by PKA activation. No effect of PKA activation on ASP uptake; when associated with A-286; A-292; A-296 and A-328. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition. Significant reduction of ASP uptake by p56(lck) tyrosine kinase-induced inhibition; when associated with A-286; A-292; A-296; A-328. No significant effect on trafficking from intracellular pools to the cell membrane; when associated with A-286; A-292; A-296 and A-328. suppresses phosphorylation by PKC; when associated with A-286; A-292; A-296 and A-328.

8sc2A Human oct1 bound to diltiazem in inward-open conformation (see paper)
25% identity, 78% coverage: 82:421/435 of query aligns to 144:444/453 of 8sc2A

query
sites
8sc2A

G

G

G

S

I

L

V

G

F

V

G

G

H

Y

I

F

G

A

D

D

R

R

Y

F

G

G

R

R

R

K

A

L

S

C

L

L

V

L

W

G

T

T

L

V

L

L

I

V

M

N

G

A

F

V

A

S

T

G

F

V

G

L

I

M

G

A

L

F

L

S

P

P

T

N

Y

Y

D

-

H

-

V

-

G

-

L

-

W

-

A

-

P

-

A

M

A

S

L

M

V

L

A

L

L

F

R

R

L

L

Q

Q

G

G

I

L

A

V

S

S

G

K

G

G

E

N

W
|
W

G

M

G

A

G

G

V

Y

L

T

M

L

I

I

S

T

E

E

S

F

A

V

P

G

P

S

E

G

K

S

R

R

G

R

Y

T

Y

V

A

A

A

I

W

M

S

Y

Q
|
Q

L

M

G

A

V
x
F

G

T

G

V

G

G

F

L

V

V

-

A

L

L

S

T

S

G

A

L

A

A

F

Y

L

-

G

-

V

-

Q

-

A

A

L

L

P

P

H

H

D

W

Q

R

F

W

V

L

S

Q

W

L

G

A

W

V

R

S

L

L

P

P

F

-

L

-

A

-

S

-

I

T

L

F

I

L

F

F

A

L

L

L

G

Y

V

Y

Y

W

I

C

R

V

H

P

N

E

L

S

P

P

E

R

S

S

R

P

D

S

F

F

E

A

N

D

A

L

D

F

K

R

A

T

G

P

K

R

T

L

A

-

H

-

M

-

P

-

V

-

E

-

V

-

I

-

K

-

R

R

H

K

P

R

K

T

E

F

I

I

L

L

M

M

A

-

M

-

G

-

L

-

R

-

V

-

A

-

E

-

N

-

G

-

A

-

Y

Y

I

L

F
x
W

L

F

A

T

F

D

S
|
S

L

V

V

L

Y

Y

G

Q

K

G

-

L

-

I

-

L

Y

H

A

M

G

G

I

A

P

T

S

S

S

G

T

N

M

L

L

Y

T

L

G

D

V

F

M

L

L

Y

A

S

M

A

V

L

V

V

E

E

M

I

V

P

A

G

M

A

L

F

L

I

W

A

G

L

K

I

L

T

S

I

D

D

R

R

I

V

G

G

R

R

K

-

P

-

V

I

Y

Y

M

P

I

M

G

A

A

M

I

S

S

N

L

L

V

L

A

A

M

G

A

A

F

A

P

C

F

L

F

V

W

M

L

I

L

F

N

-

T

-

H

I

S

S

T

P

P

D

L

L

I

H

W

W

L

L

A

N

L

I

V

I

L

I

G

-

T

-

A

-

V

M

C

C

H

V

G

G

A

R

M

M

-

G

-

I

-

T

-

I

-

A

I
|
I

G

Q

T

M

L

I

P

C

A

L

L

V

V

N

G

A

E

E

L

L

F

Y

S

P

T

T

E

F

V

V

R

R

Y

N

S

L

G

G

V

V

A

M

L

V

G

C

H

S

E

S

V

L

A

C

S

D

I

I

F

-

A

G

G

G

G

I

L

I

S

T

P

P

L

F

I

I

A

V

T

F

A

R

L

L

L

R

A

E

H

V

Y

W

H

Q

A

A

Y

L

W

-

P

P

V

L

A

I

V

L

F

F

L

-

I

A

V

V

L

L

G

G

L

L

I

L

binding Diltiazem: W199 (= W145), Q223 (= Q169), F226 (≠ V172), W293 (≠ F268), S297 (= S272), I385 (= I359)

Sites not aligning to the query:

binding Diltiazem: 18

8sc3A Human oct1 bound to fenoterol in inward-open conformation (see paper)
25% identity, 78% coverage: 82:421/435 of query aligns to 144:435/445 of 8sc3A

query
sites
8sc3A

G

G

G

S

I

L

V

G

F

V

G

G

H

Y

I

F

G

A

D

D

R

R

Y

F

G

G

R

R

R

K

A

L

S

C

L

L

V

L

W

G

T

T

L

V

L

L

I

V

M

N

G

A

F

V

A

S

T

G

F

V

G

L

I

M

G

A

L

F

L

S

P

P

T

N

Y

Y

D

-

H

-

V

-

G

-

L

-

W

-

A

-

P

-

A

M

A

S

L

M

V

L

A

L

L

F

R

R

L

L

Q

Q

G

G

I

L

A

V

S

S

G

K

G

G

E

N

W
|
W

G
x
M

G

A

G

G

V

Y

L

T

M

L

I

I

S

T

E

E

S

F

A

V

P

G

P

S

E

G

K

S

R

R

G

R

Y

T

Y

V

A

A

A

I

W

M

S

Y

Q
|
Q

L

M

G

A

V
x
F

G

T

G

V

G

G

F

L

V

V

-

A

L

L

S

T

S

G

A

L

A

A

F

Y

L

-

G

-

V

-

Q

-

A

A

L

L

P

P

H

H

D

-

Q

-

F

-

V

-

S

-

W

W

G

R

W

W

R

-

L

L

P

Q

F

L

L

A

A

V

S

S

I

L

L

P

I

T

F

F

A

L

L

F

G

L

V

L

Y

Y

I

Y

R

-

H

-

N

-

L

-

P

P

E

S

S

F

R

A

D

D

F

L

E

F

N

R

A

T

D

P

K

R

A

L

G

R

K

K

T

R

A

T

H

F

M

I

P

L

V

M

V

Y

E

L

V
x
W

I

F

K

T

R

D

H

S

P

V

-

L

-
x
Y

K

Q

E

G

I

L

L

I

M

L

A

H

M

M

G

G

L

-

R

-

V

-

A

A

E

T

N

S

G

G

G

N

A

L

Y

Y

I

L

F

-

L

-

A

-

F

-

S

D

L

F

V

L

Y

Y

G

S

K

A

Y

L

A

V

G

E

I

I

P

P

S

-

T

-

M

-

L

-

T

-

G

G

V

A

M

F

L

I

A

A

M

L

V

I

V

T

E

-

M

-

V

-

A

-

M

-

L

-

W

-

G

-

K

-

L

-

S

I

D

D

R

R

I

V

G

G

R

R

K

-

P

-

V

I

Y

Y

M

P

I

M

G

A

A

M

I

S

S

N

L

L

V

L

A

A

M

G

A

A

F

A

P

C

F

L

F

V

W

M

L

I

L

F

N

-

T

-

H

I

S

S

T

P

P

D

L

L

I

H

W

W

L

L

A

N

L

I

V

I

L

I

G

-

T

-

A

-

V

M

C

C

H

V

G

G

A

R

M

M

-

G

-

I

-

T

-

I

-

A

I
|
I

G

Q

T

M

L

I

P
x
C

A

L

L

V

V

N

G

A

E

E

L

L

F

Y

S

P

T

T

E

F

V

V

R

R

Y

N

S

L

G

G

V

V

A

M

L

V

G

C

H

S

E

S

V

L

A

C

S

D

I

I

F

-

A

G

G

G

G

I

L

I

S

T

P

P

L

F

I

I

A

V

T

F

A

R

L

L

L

R

A

E

H

V

Y

W

H

Q

A

A

Y

L

W

-

P

P

V

L

A

I

V

L

F

F

L

-

I

A

V

V

L

L

G

G

L

L

I

L

binding Fenoterol: W199 (= W145), M200 (≠ G146), Q223 (= Q169), F226 (≠ V172), W284 (≠ V243), Y291 (vs. gap), I376 (= I359), C380 (≠ P363)

8sc6A Human oct1 bound to thiamine in inward-open conformation (see paper)
25% identity, 78% coverage: 82:421/435 of query aligns to 144:437/447 of 8sc6A

query
sites
8sc6A

G

G

G

S

I

L

V

G

F

V

G

G

H

Y

I

F

G

A

D

D

R

R

Y

F

G

G

R

R

R

K

A

L

S

C

L

L

V

L

W

G

T

T

L

V

L

L

I

V

M

N

G

A

F

V

A

S

T

G

F

V

G

L

I

M

G

A

L

F

L

S

P

P

T

N

Y

Y

D

-

H

-

V

-

G

-

L

-

W

-

A

-

P

-

A

M

A

S

L

M

V

L

A

L

L

F

R

R

L

L

Q

Q

G

G

I

L

A

V

S

S

G

K

G

G

E

N

W

W

G

M

G

A

G

G

V

Y

L

T

M

L

I

I

S

T

E

E

S

F

A

V

P

G

P

S

E

G

K

S

R

R

G

R

Y

T

Y

V

A

A

A

I

W

M

S

Y

Q
|
Q

L

M

G

A

V
x
F

G

T

G

V

G

G

F

L

V

V

-

A

L

L

S

T

S

G

A

L

A

A

F

Y

L

-

G

-

V

-

Q

-

A

A

L

L

P

P

H

H

D

-

Q

-

F

-

V

-

S

-

W

W

G

R

W

W

R

-

L

L

P

Q

F

L

L

A

A

V

S

S

I

L

L

P

I

T

F

F

A

L

L

F

G

L

V

L

Y

Y

I

-

R

-

H

Y

N

W

L

S

P

P

E

S

S

F

R

A

D

D

F

L

E

F

N

R

A

T

D

P

K

R

A

L

G

R

K

K

T

R

A

T

H

F

M

I

P

L

V

M

V

Y

E

L

V

W

I

F

K

T

R

D

H

S

P

V

-

L

-
x
Y

K
x
Q

E

G

I

L

L

I

M

L

A

H

M

M

G

G

L

-

R

-

V

-

A

A

E

T

N

S

G

G

G

N

A

L

Y

Y

I

L

F

-

L

-

A

-

F

-

S

D

L

F

V

L

Y

Y

G

S

K

A

Y

L

A

V

G
x
E

I

I

P

P

S

-

T

-

M

-

L

-

T

-

G

G

V

A

M

F

L

I

A

A

M

L

V

I

V

T

E

-

M

-

V

-

A

-

M

-

L

-

W

-

G

-

K

-

L

-

S

I

D

D

R

R

I

V

G

G

R

R

K

-

P

-

V

I

Y

Y

M

P

I

M

G

A

A

M

I

S

S

N

L

L

V

L

A

A

M

G

A

A

F

A

P

C

F

L

F

V

W

M

L

I

L

F

N

-

T

-

H

I

S

S

T

P

P

D

L

L

I

H

W

W

L

L

A

N

L

I

V

I

L

I

G

-

T

-

A

-

V

M

C

C

H

V

G

G

A

R

M

M

-

G

-

I

-

T

-

I

-

A

I
|
I

G
x
Q

T

M

L

I

P

C

A

L

L

V

V

N

G

A

E

E

L

L

F

Y

S

P

T

T

E

F

V

V

R

R

Y

N

S

L

G

G

V

V

A

M

L

V

G

C

H

S

E

S

V

L

A

C

S

D

I

I

F

-

A

G

G

G

G

I

L

I

S

T

P

P

L

F

I

I

A

V

T

F

A

R

L

L

L

R

A

E

H

V

Y

W

H

Q

A

A

Y

L

W

-

P

P

V

L

A

I

V

L

F

F

L

-

I

A

V

V

L

L

G

G

L

L

I

L

binding 3-(4-amino-2-methyl-pyrimidin-5-ylmethyl)-5-(2-hydroxy-ethyl)-4-methyl-thiazol-3-ium: Q223 (= Q169), F226 (≠ V172), Y293 (vs. gap), Q294 (≠ K249), E318 (≠ G280), I378 (= I359), Q379 (≠ G360)

Q8MJI6 Solute carrier family 22 member 2; Organic cation transporter 2 from Oryctolagus cuniculus (Rabbit) (see paper)
25% identity, 46% coverage: 70:271/435 of query aligns to 153:334/554 of Q8MJI6

query
sites
Q8MJI6

A

S

A

A

F

V

A

N

M

I

G

G

F

F

V

F

A

-

R

-

P

-

L

I

G

G

G

S

I

V

V

G

F

I

G

G

H

Y

I

L

G

A

D

D

R

R

Y

F

G

G

R

R

R

K

A

L

S

C

L

L

V

L

W

V

T

T

L

I

L

L

I

I

M

N

G

A

F

A

A

A

T

G

F

V

G

L

I

M

G

A

L

V

L

S

P

P

T

N

Y

Y

D

T

H

-

V

-

G

-

L

-

W

W

A

-

P

-

A

-

L

M

V

L

A

I

L

F

R

R

L

L

L

I

Q

Q

G

G

I

L

A

V

S

S

G

K

G

A

E

G

W

W

G

L

G

I

G

G

V

Y

L

I

M

L

I

I

S

T

E

E

S

F

A

V

P

G

P

L

E

N

K

Y

R

R

G

R

Y

T

Y

V

A

G

A

I

W

L

S

Y

Q

Q

L

V

G

A

V

F

G

T

G

V

G

G

-

L

F

L

V

V

L

L

S

A

S

G

A

V

A

A

F

Y

L

-

G

-

V

-

Q

-

A

A

L

L

P

P

H

R

D

W

Q

R

F

W

V

L

S

Q

W

L

G

T

W

V

R

T

L

L

P

P

F

Y

L

F

A

C

S

F

I

L

L

L

I

Y

F

Y

A

W

L

C

G

-

V

-

Y

-

I

-

R

-

H

-

N

-

L

I

P

P

E

E

S

S

R

P

D

R

F

W

E

L

N

I

A

S

D

Q

K

N

A

K

G

N

K

A

T

K

A

A

H

M

M

R

P

I

V

M

V

E

E

H

V

I

I

A

K

K

R

K

H

N

P

G

K

K

E

S

I

L

L

P

M

V

A

S

M

L

-

Q

G

S

L

L

R

R

V

A

A

A

E

E

N

D

G

V

G

G

A

E

Y

K

I

L

F

N

L

P

A

S

F

F

Sites not aligning to the query:

71 N→Q: Higher affinity for TEA. Loss of plasma membrane localization; when associated with Q-76. Loss of plasma membrane localization; when associated with Q-112. Loss of plasma membrane localization; when associated with Q-76 and Q-112.
96 N→Q: Higher affinity for TEA and lower Vmax.
112 N→Q: Higher affinity for TEA, lower Vmax and loss of plasma membrane localization.

8et9A Cryo-em structure of the organic cation transporter 2 in complex with 1-methyl-4-phenylpyridinium (see paper)
25% identity, 42% coverage: 70:251/435 of query aligns to 152:312/517 of 8et9A

query
sites
8et9A

A

A

F

V

A

N

M

V

G

G

F

F

V

F

A

-

R

-

P

-

L

V

G

G

G

S

I

M

V

S

F

I

G

G

H

Y

I

I

G

A

D

D

R

R

Y

F

G

G

R

R

R

K

A

L

S

C

L

L

V

L

W

V

T

T

L

I

L

L

I

I

M

N

G

A

F

I

A

S

T

G

F

V

G

L

I

M

G

A

L

V

L

A

P

P

T

N

Y

Y

D

T

H

-

V

-

G

-

L

-

W

W

A

-

P

-

A

-

L

M

V

L

A

I

L

F

R

R

L

L

L

I

Q

Q

G

G

I

L

A

V

S

S

G

K

G

G

E

G

W
|
W

G

L

G

I

G

G

V

Y

L

T

M

L

I

P

S

T

E

E

S

F

A

V

P

G

P

L

E

E

K

Y

R

R

G

R

Y

T

Y

V

A

G

A

I

W

L

S

Y

Q
|
Q

L

M

G

A

V
x
F

G

S

G

V

G

G

-

L

F

L

V

V

L

L

S

A

S

G

A

V

A

A

F

Y

L

-

G

-

V

-

Q

-

A

A

L

I

P

P

H

H

D

W

Q

R

F

W

V

L

S

Q

W

L

G

A

W

V

R

T

L

L

P

P

F

N

L

F

A

F

S

F

I

L

L

L

I

Y

F

Y

A

W

L

C

G

-

V

-

Y

-

I

-

R

-

H

-

N

-

L

I

P

P

E

E

S

S

R

P

D

R

F

W

E

L

N

I

A

S

D

Q

K

N

A

K

G

N

K

A

T

K

A

A

H

M

M

K

P

I

V

I

V

K

E

H

V

I

I

A

K

K

R

K

H

N

P

G

K

K

E

K

I

L

binding 1-methyl-4-phenylpyridin-1-ium: W216 (= W145), Q240 (= Q169), F243 (≠ V172)

Sites not aligning to the query:

binding 1-methyl-4-phenylpyridin-1-ium: 430

Q9R0W2 Solute carrier family 22 member 2; Organic cation transporter 2; rOCT2 from Rattus norvegicus (Rat) (see paper)
22% identity, 77% coverage: 81:414/435 of query aligns to 162:500/555 of Q9R0W2

query
sites
Q9R0W2

L

I

G

G

G

A

I

M

V

M

F

I

G

G

H

Y

I

L

G

A

D

D

R

R

Y

F

G

G

R

R

R

K

A

F

S

C

L

L

V

L

W

V

T

T

L

I

L

L

I

I

M

N

G

A

F

I

A

S

T

G

F

A

G

L

I

M

G

A

L

I

L

S

P

P

T

N

Y

Y

D

-

H

-

V

-

G

-

L

-

W

-

A

-

P

-

A

A

A

W

L

M

V

L

A

V

L

F

R

R

L

F

L

L

Q

Q

G

G

I

L

A

V

S

S

G

K

G

A

E

G

W

W

G

L

G

I

G

G

V

Y

L

I

M

L

I

I

S

T

E

E

S

F

A

V

P

G

P

L

E

G

K

Y

R

R

G

R

Y

M

Y

V

A

G

A

I

W

C

S

Y

Q

Q

L

I

G

A

V

F

G

T

G

V

G

G

-

L

F

L

V

I

L

L

S

A

S

G

A

V

A

A

F

Y

L

V

G

-

V

-

Q

-

A

-

L

I

P

P

H

N

D

W

Q

R

F

W

V

L

S

Q

W

F

G

A

W

V

R

T

L

L

P

P

F

N

L

F

A

C

S

F

I

L

L

L

I

Y

F

F

A

W

L

C

G

-

V

-

Y

-

I

-

R

-

H

-

N

-

L

I

P

P

E

E

S

S

R

P

D

R

F

W

E

L

N

I

A

S

D

Q

K

N

A

K

G

I

K

V

T

K

A

A

H

M

M

K

P

I

V

I

V

K

E

H

V

I

I

A

K

K

R

K

H

N

P

G

K

K

E

S

I

V

L

P

M

V

A

S

M

L

-

Q

G

N

L

L

R

T

V

P

A

D

E

E

N

D

G

A

G

G

A

K

Y

K

I

L

F

N

L

P

A

S

F

F

-

L

S

D

L

L

V

V

Y

R

G

T

K

P

-

Q

-

I

-

R

-

K

-

H

-

T

-

L

-

I

-

L

-

M

Y

Y

A

N

G

W

I

F

P

T

S

S

T

V

M

L

L

Y

T

Q

G

G

V

L

M

I

L

M

A

H

M

M

-

G

-

L

-

A

-

G

-

D

-

N

-

I

-

Y

-

L

-

D

-

F

-

Y

-

S

-

A

V

L

V

V

E

E

M

F

V

P

A

A

M

A

L

F

L

I

W

I

G

I

K

L

L

T

S

I

D

D

R

R

I

V

G

G

R

R

K

R

P

Y

V

P

Y

W

M

A

I

V

-

S

-

N

-

M

-

V

-

A

G

G

A

A

I

A

S

C

L

L

V

A

A

S

M

V

A

F

F

I

P

P

F

-

W

-

L

-

N

-

T

-

H

-

S

-

T

D

P

D

L

L

I

Q

W

W

L

L

A

K

L

I

V

-

L

-

G

-

T

T

A

I

V

A

C

C

H

L

G

G

A

R

M

M

I

G

G

I

T

T

L

M

P

A

-

Y

-

E

-

M

-

V

A
x
C

L

L

V

V

G

N

-

A

E

E

L

L

F

Y

S

P

T

T

E

Y

V

I

R

R

Y

N

S

L

G

G

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V

A

L

L

V

G

C

H

S

E

S

V

M

A

C

S

D

I

I

F

-

A

G

G

G

G

I

L

I

S

T

P

P

L

F

I

L

A

V

T

Y

A

R

L

L

L

T

A

D

H

I

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W

H

M

A

E

Y

F

W

-

P

P

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L

A

V

V

V

F

F

C451 (≠ A364) Involved in recognition of organic cations and participates in structural changes that occur during translocation of organic cations; mutation to M: Transport activity strongly reduced.

Query Sequence

>H281DRAFT_01650 FitnessBrowser__Burk376:H281DRAFT_01650
MSTIQSSPAAERNAAMPDRALKRIVVASVAGNAMEWYDFFVYGTAAALVFGQLFFPANAD
PLIGTLGAFAAFAMGFVARPLGGIVFGHIGDRYGRRASLVWTLLIMGFATFGIGLLPTYD
HVGLWAPAALVALRLLQGIASGGEWGGGVLMISESAPPEKRGYYAAWSQLGVGGGFVLSS
AAFLGVQALPHDQFVSWGWRLPFLASILIFALGVYIRHNLPESRDFENADKAGKTAHMPV
VEVIKRHPKEILMAMGLRVAENGGAYIFLAFSLVYGKYAGIPSSTMLTGVMLAMVVEMVA
MLLWGKLSDRIGRKPVYMIGAISLVAMAFPFFWLLNTHSTPLIWLALVLGTAVCHGAMIG
TLPALVGELFSTEVRYSGVALGHEVASIFAGGLSPLIATALLAHYHAYWPVAVFLIVLGL
ITVVTLRFTHETRQR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory