SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_01655 FitnessBrowser__Burk376:H281DRAFT_01655 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

Q01468 2-hydroxymuconate tautomerase; 4-oxalocrotonate tautomerase; 4-OT; EC 5.3.2.6 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
36% identity, 48% coverage: 1:59/124 of query aligns to 1:59/63 of Q01468

query
sites
Q01468

M
|
M

P
|
P

T

I

L

A

E

Q

V

I

Y

H

L

I

P

L

Q

E

G

G

H

R

S

S

D

D

E

E

R

Q

K

K

A

E

S

T

L

L

I

I

Q

R

G

E

L

V

T

S

Q

E

A

A

T

I

V

S

D

R

A

S

I

L

G

D

A

A

P

P

A

L

E

T

S

S

V

V

R

R

I

V

L

I

S

T

E

E

L

M

P

A

V

K

S

G

H

H

I

F

G

G

L

I

G

G

K

E

L

L

T

A

A

S

M1 (= M1) modified: Initiator methionine, Removed
P2 (= P2) active site, Proton acceptor; via imino nitrogen

5tigA Crystal structure of 4-oxalocrotonate tautomerase inactivated by brhpd (see paper)
34% identity, 47% coverage: 2:59/124 of query aligns to 1:58/62 of 5tigA

query
sites
5tigA

P
|
P

T

I

L

A

E

Q

V

I

Y

H

L

I

P

L

Q

E

G

G

H

R

S

S

D

D

E

E

R

Q

K

K

A

E

S

T

L

L

I

I

Q

R

G

E

L

V

T

S

Q

E

A

A

T

I

V

S

D

R

A

S

I

L

G

D

A

A

P

P

A

L

E

T

S

S

V

V

R
|
R

I

V

L

I

S

T

E

E

L

M

P

A

V

K

S

G

H

H

I
x
F

G

G

L

I

G

G

K

E

L

L

T

A

A

S

active site: P1 (= P2), R39 (= R40), F50 (≠ I51)
binding (3E)-5-hydroxy-2-oxopent-3-enoic acid: F50 (≠ I51)

Sites not aligning to the query:

active site: 61
binding (3E)-5-hydroxy-2-oxopent-3-enoic acid: 61

1bjpA Crystal structure of 4-oxalocrotonate tautomerase inactivated by 2- oxo-3-pentynoate at 2.4 angstroms resolution (see paper)
34% identity, 47% coverage: 2:59/124 of query aligns to 1:58/62 of 1bjpA

query
sites
1bjpA

P
|
P

T
x
I

L

A

E

Q

V

I

Y

H

L

I

P

L

Q

E

G

G

H
x
R

S

S

D

D

E

E

R

Q

K

K

A

E

S

T

L

L

I

I

Q

R

G

E

L

V

T

S

Q

E

A

A

T

I

V

S

D

R

A

S

I

L

G

D

A

A

P

P

A

L

E

T

S

S

V

V

R
|
R

I

V

L

I

S

T

E

E

L

M

P

A

V

K

S

G

H

H

I
x
F

G

G

L

I

G

G

K

E

L

L

T

A

A

S

active site: P1 (= P2), R11 (≠ H12), R39 (= R40), F50 (≠ I51)
binding 2-oxo-3-pentenoic acid: P1 (= P2), I2 (≠ T3), F50 (≠ I51)

Sites not aligning to the query:

binding 2-oxo-3-pentenoic acid: 61

6bgnA Crystal structure of 4-oxalocrotonate tautomerase after incubation with 5-fluoro-2-hydroxy-2,4-pentadienoate (see paper)
34% identity, 47% coverage: 2:59/124 of query aligns to 1:58/59 of 6bgnA

query
sites
6bgnA

P
|
P

T

I

L

A

E

Q

V

I

Y

H

L

I

P

L

Q

E

G

G

H

R

S

S

D

D

E

E

R

Q

K

K

A

E

S

T

L

L

I

I

Q

R

G

E

L

V

T

S

Q

E

A

A

T

I

V

S

D

R

A

S

I

L

G

D

A

A

P

P

A

L

E

T

S
|
S

V

V

R
|
R

I

V

L

I

S

T

E

E

L

M

P

A

V

K

S

G

H

H

I
x
F

G

G

L

I

G

G

K

E

L

L

T

A

A

S

active site: P1 (= P2), R39 (= R40), F50 (≠ I51)
binding 5-fluoranyl-2-oxidanylidene-pentanoic acid: P1 (= P2), S37 (= S38)

6ghwC Substituting the prolines of 4-oxalocrotonate tautomerase with non- canonical analogue (2s)-3,4-dehydroproline (see paper)
36% identity, 45% coverage: 2:57/124 of query aligns to 1:56/57 of 6ghwC

query
sites
6ghwC

P
|
P

T

I

L

A

E

Q

V

I

Y

H

L

I

P

L

Q

E

G

G

H

R

S

S

D

D

E

E

R

Q

K

K

A

E

S

T

L

L

I

I

Q

R

G

E

L

V

T

S

Q

E

A

A

T

I

V

S

D
x
R

A

S

I

L

G
x
D

A

A

P

P

A

L

E

T

S

S

V

V

R
|
R

I

V

L

I

S

T

E

E

L

M

P

A

V

K

S

G

H

H

I
x
F

G

G

L

I

G

G

K

E

L

L

active site: P1 (= P2), R39 (= R40), F50 (≠ I51)
binding calcium ion: R29 (≠ D30), D32 (≠ G33)

5cloM Crystal structure of a 4-oxalocrotonate tautomerase mutant in complex with nitrostyrene at 2.3 angstrom (see paper)
36% identity, 45% coverage: 2:57/124 of query aligns to 1:56/57 of 5cloM

query
sites
5cloM

P
|
P

T

I

L

A

E

Q

V

I

Y

H

L

I

P

L

Q

E

G

G

H

R

S

S

D

D

E

E

R

Q

K

K

A

E

S

T

L

L

I

I

Q

R

G

E

L

V

T

S

Q

E

A

A

T

I

V

S

D

R

A

S

I

L

G

D

A

A

P

P

A

L

E

T

S
|
S

V

V

R
|
R

I

V

L

I

S

T

E

E

L

Y

P

A

V

K

S

G

H

H

I
x
A

G

G

L

I

G

G

K

E

L

L

active site: P1 (= P2), R39 (= R40), A50 (≠ I51)
binding trans beta nitrostyrene: P1 (= P2), S37 (= S38)

7ms8A Crystal structure of y103f mutant of cg10062 with a covalent intermediate of the hydration of acetylenecarboxylic acid (see paper)
26% identity, 85% coverage: 13:117/124 of query aligns to 13:117/146 of 7ms8A

query
sites
7ms8A

S

S

D

R

E

E

R

A

K

K

A

Q

S

R

L

I

I

A

Q

E

G

A

L

I

T

T

Q

D

A

A

T

H

V

H

D

E

A

L

I

A

G

H

A

A

P

P

A

K

E

Y

S

L

V

V

R

Q

I

V

L

I

L

F

S

N

E

E

L

V

P

E

V

P

S

D

H

S

I

Y

G

F

L

I

G

A

K

Q

L

S

T

A

A

S

A

E

A

N

L

H

P

I

V

W

I

V

I

Q

A

A

I

T

L

I

I
x
R

A

S

G

G

R

R

T

T

E

E

A

K

Q

Q

K

K

E

E

A

E

L

L

I

L

A

L

H

R

L

L

S

T

E

Q

T

E

S

I

A

A

S

L

V

I

L

L

N

G

V

I

P

P

L

N

Q

E

S

E

T

V

R

W

V

V

M

F

I

I

K

T

D

E

I

I

P

P

S

G

T

S

D

N

F
x
M

G

T

L

E

G

Y

G

G

K

R

binding 3-hydroxy-propanoic acid: R70 (≠ I70), M112 (≠ F112)

Sites not aligning to the query:

binding 3-hydroxy-propanoic acid: 1

P70994 2-hydroxymuconate tautomerase; (2Z,4E)-2-hydroxyhexa-2,4-dienedioate keto-enol isomerase; 4-oxalocrotonate tautomerase; 4-OT; EC 5.3.2.6 from Bacillus subtilis (strain 168) (see paper)
30% identity, 45% coverage: 1:56/124 of query aligns to 1:56/62 of P70994

query
sites
P70994

M

M

P
|
P

T

Y

L

V

E

T

V

V

Y

K

L

M

P

L

Q

E

G

G

H
x
R

S

T

D

D

E

E

R

Q

K

K

A

R

S

N

L

L

I

V

Q

E

G

K

L

V

T

T

Q

E

A

A

T

V

V

K

D

E

A

T

I

T

G

G

A

A

P

S

A

E

E

E

S

K

V

I

R

V

I

V

L

F

L

I

S

E

E

E

L

M

P

R

V

K

S

D

H

H

I

Y

G

A

L

V

G

A

G

G

K

K

P2 (= P2) active site, Proton acceptor; via imino nitrogen; mutation to A: Absence of tautomerase activity using 2-hydroxymuconate. For 2-hydroxy-2,4-pentadienoate and phenylenolpyruvate, a strong decrease of catalytic efficiency and a 2-fold decrease of affinity is observed.
R12 (≠ H12) mutation to A: Absence of tautomerase activity using 2-hydroxymuconate. For 2-hydroxy-2,4-pentadienoate and phenylenolpyruvate, a strong decrease of catalytic efficiency and a 2-fold decrease of affinity is observed.

Query Sequence

>H281DRAFT_01655 FitnessBrowser__Burk376:H281DRAFT_01655
MPTLEVYLPQGHSDERKASLIQGLTQATVDAIGAPAESVRILLSELPVSHIGLGGKLTAA
ALPVIIAILIAGRTEAQKEALIAHLSETSASVLNVPLQSTRVMIKDIPSTDFGLGGKTAR
SLGR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory