SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_01887 FitnessBrowser__Burk376:H281DRAFT_01887 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

P04968 L-threonine dehydratase biosynthetic IlvA; Threonine deaminase; EC 4.3.1.19 from Escherichia coli (strain K12) (see paper)
53% identity, 99% coverage: 3:281/281 of query aligns to 235:513/514 of P04968

query
sites
P04968

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S315 (= S83) binding

Sites not aligning to the query:

62 modified: N6-(pyridoxal phosphate)lysine
89 binding
188:192 binding

1tdjA Threonine deaminase (biosynthetic) from e. Coli (see paper)
52% identity, 99% coverage: 3:281/281 of query aligns to 231:493/494 of 1tdjA

query
sites
1tdjA

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active site: G237 (= G9), L310 (≠ T82), S311 (= S83)
binding pyridoxal-5'-phosphate: G237 (= G9), E282 (= E54), S311 (= S83), G312 (= G84)

Sites not aligning to the query:

active site: 58, 83, 209, 213, 215
binding pyridoxal-5'-phosphate: 57, 58, 85, 184, 185, 186, 187

1ve5D Crystal structure of t.Th. Hb8 threonine deaminase
35% identity, 30% coverage: 7:90/281 of query aligns to 199:279/280 of 1ve5D

query
sites
1ve5D

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active site: G201 (= G9), L271 (≠ T82), S272 (= S83)
binding pyridoxal-5'-phosphate: V202 (≠ T10), E246 (= E54), T248 (≠ A56), S272 (= S83), G273 (= G84)

Sites not aligning to the query:

active site: 50, 56, 72, 172, 176, 178
binding calcium ion: 172, 176, 178
binding pyridoxal-5'-phosphate: 49, 50, 74, 147, 148, 149, 150

1ve5A Crystal structure of t.Th. Hb8 threonine deaminase
35% identity, 30% coverage: 7:90/281 of query aligns to 227:307/308 of 1ve5A

query
sites
1ve5A

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active site: G229 (= G9), L299 (≠ T82), S300 (= S83)
binding pyridoxal-5'-phosphate: E274 (= E54), T276 (≠ A56), S300 (= S83), G301 (= G84)

Sites not aligning to the query:

active site: 50, 56, 72, 200, 204, 206
binding calcium ion: 200, 204, 206
binding pyridoxal-5'-phosphate: 49, 50, 74, 175, 176, 177, 178

2zr8A Crystal structure of modified serine racemase complexed with serine (see paper)
31% identity, 31% coverage: 6:91/281 of query aligns to 229:312/319 of 2zr8A

query
sites
2zr8A

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active site: G232 (= G9), I303 (≠ T82), S304 (= S83)
binding n-(5'-phosphopyridoxyl)-d-alanine: G232 (= G9), E277 (= E54), T279 (≠ A56), S304 (= S83)
binding serine: D231 (= D8), G232 (= G9), A233 (≠ T10), Q234 (≠ A11), T235 (≠ V12)

Sites not aligning to the query:

active site: 53, 78, 204, 208, 210
binding magnesium ion: 204, 208, 210
binding n-(5'-phosphopyridoxyl)-d-alanine: 52, 53, 77, 78, 80, 81, 147, 179, 180, 181, 182
binding serine: 78, 129

2zpuA Crystal structure of modified serine racemase from s.Pombe. (see paper)
31% identity, 31% coverage: 6:91/281 of query aligns to 229:312/319 of 2zpuA

query
sites
2zpuA

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active site: G232 (= G9), I303 (≠ T82), S304 (= S83)
binding n-(5'-phosphopyridoxyl)-d-alanine: G232 (= G9), E277 (= E54), T279 (≠ A56), S304 (= S83)

Sites not aligning to the query:

active site: 53, 78, 204, 208, 210
binding magnesium ion: 204, 208, 210
binding n-(5'-phosphopyridoxyl)-d-alanine: 52, 53, 77, 78, 80, 81, 147, 179, 180, 181, 182

1wtcA Crystal structure of s.Pombe serine racemase complex with amppcp (see paper)
31% identity, 31% coverage: 6:91/281 of query aligns to 228:311/318 of 1wtcA

query
sites
1wtcA

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active site: G231 (= G9), I302 (≠ T82), S303 (= S83)
binding pyridoxal-5'-phosphate: G231 (= G9), E276 (= E54), T278 (≠ A56), S303 (= S83)

Sites not aligning to the query:

active site: 52, 77, 203, 207, 209
binding phosphomethylphosphonic acid adenylate ester: 20, 47, 48, 109, 110, 114
binding magnesium ion: 203, 207, 209
binding pyridoxal-5'-phosphate: 51, 52, 79, 178, 179, 180, 181

1v71A Crystal structure of s.Pombe serine racemase
31% identity, 31% coverage: 6:91/281 of query aligns to 228:311/318 of 1v71A

query
sites
1v71A

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active site: G231 (= G9), I302 (≠ T82), S303 (= S83)
binding pyridoxal-5'-phosphate: G231 (= G9), E276 (= E54), T278 (≠ A56), S303 (= S83), G304 (= G84)

Sites not aligning to the query:

active site: 52, 77, 203, 207, 209
binding magnesium ion: 203, 207, 209
binding pyridoxal-5'-phosphate: 51, 52, 79, 178, 179, 180, 181

O59791 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 4.3.1.17; EC 4.3.1.18; EC 5.1.1.18 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
31% identity, 31% coverage: 6:91/281 of query aligns to 233:316/323 of O59791

query
sites
O59791

F

I

S

A

D

D

G

G

T

A

A

Q

V

T

K

Q

L

H

V

L

G

G

E

N

E

Y

T

T

F

F

R

S

L

I

C

I

S

K

E

E

Y

K

L

V

D

D

D

D

V

I

L

L

L

T

V

V

N

S

T

D

D

E

A

E

L

L

C

I

A

D

A

C

I

L

K

K

D

F

V

Y

F

A

Q

A

D

R

T

M

R

K

S

I

V

V

L

V

E

E

P

P

A

T

G

G

S

C

L

L

A

S

V

F

A

A

G

A

A

A

K

R

Q

-

Y

-

A

A

E

M

R

K

E

E

G

K

I

L

E

K

N

N

Q

K

T

R

L

I

I

G

A

I

I

I

T

I

S

S

G

G

A

G

N

N

M

V

N

D

F

I

D

E

R

R

Sites not aligning to the query:

82 S→A: Loss of racemase activity. Reduces D-serine dehydratase activity by 99%. Slightly reduced L-serine dehydratase activity.

Query Sequence

>H281DRAFT_01887 FitnessBrowser__Burk376:H281DRAFT_01887
NEVGLFSDGTAVKLVGEETFRLCSEYLDDVLLVNTDALCAAIKDVFQDTRSVLEPAGSLA
VAGAKQYAEREGIENQTLIAITSGANMNFDRMRFVAERAEVGEAREAVFAVTIPEERGSF
RRFCELVGTRSVTEFNYRIAQAESAHIFVGVQIRNRSESAQIAGAFEAHNFATVDLTFDE
LSKQHIRYMVGGRSPLAHDERLFRFEFPERPGALMKFLSSMAPDWNISLFHYRNQGADYS
SILVGIQVPQTDNAEFERFLATLGYPHWEETHNPVYRLFLA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory