SitesBLAST

1a56A Primary sequence and solution conformation of ferricytochromE C-552 from nitrosomonas europaea, nmr, mean structure refined with explicit hydrogen bond constraints (see paper)
49% identity, 65% coverage: 36:110/116 of query aligns to 4:78/81 of 1a56A

query
sites
1a56A

I

L

A

A

N

K

A

K

N

N

A
x
N

C
|
C

M

I

G

A

C
|
C

H
|
H

A

Q

V

V

D

E

R

T

K

K

L

V

V
|
V

G
|
G

P
|
P

S

A

F

L

Q

K

Q

D

I

I

A

A

R

K

Y

Y

K

A

G

D

D

K

A

D

Q

D

A

A

P

A

A

T

K

Y

L

L

A

A

R

G

K
|
K

V
x
I

K

K

D

G

G

G

G
x
S

S

S

G

G

V

V

W
|
W

G

G

M
x
Q

I
|
I

P

P

M
|
M

P

P

A

P

H
x
N

Q
x
V

S
x
N

M

V

S

S

D

D

A

A

D

D

I

A

R

K

A

A

V

L

V

A

D

D

W

W

V

I

L

L

binding heme c: N9 (≠ A41), C10 (= C42), C13 (= C45), H14 (= H46), V21 (= V53), G22 (= G54), P23 (= P55), K45 (= K77), I46 (≠ V78), S50 (≠ G82), W54 (= W86), Q56 (≠ M88), I57 (= I89), M59 (= M91), N62 (≠ H94), V63 (≠ Q95), N64 (≠ S96)

P00099 Cytochrome c-551; Cytochrome C8; Cytochrome c551 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
37% identity, 84% coverage: 14:111/116 of query aligns to 9:102/104 of P00099

query
sites
P00099

M
x
L

F
x
L

G
x
A

A
x
T

A
x
G

S
x
T

L
|
L

F
x
L

V
x
A

S
x
Q

T
x
G

A
|
A

Y
x
W

A
|
A

A
x
E

D
|
D

M

-

P

-

S

-

G
x
P

Q
x
E

R
x
V

I
x
L

A
x
F

N
x
K

A
x
N

N
x
K

A
x
G

C
|
C

M
x
V

G
x
A

C
|
C

H
|
H

A
|
A

V
x
I

D
|
D

R
x
T

K
|
K

L
x
M

V
|
V

G
|
G

P
|
P

S
x
A

F
x
Y

Q
x
K

Q
x
D

I
x
V

A
|
A

R
x
K

Y
x
F

K
x
A

G
|
G

D
x
Q

A
|
A

Q
x
G

A
|
A

P
x
E

A
|
A

K
x
E

L
|
L

A
|
A

R
x
Q

K
x
R

V
x
I

K
|
K

D
x
N

G
|
G

G
x
S

S
x
Q

G
|
G

V
|
V

W
|
W

G
|
G

M
x
P

I
|
I

P
|
P

M
|
M

P
|
P

A
x
P

H
x
N

Q

-

S
x
A

M
x
V

S
|
S

D
|
D

A
x
D

D
x
E

I
x
A

R
x
Q

A
x
T

V
x
L

V
x
A

D
x
K

W
|
W

V
|
V

L
|
L

A
x
S

H38 (= H46) binding axial binding residue
M83 (= M91) binding axial binding residue

Sites not aligning to the query:

1:22 signal peptide
23:104 modified: mature protein, Cytochrome c-551

2exvA Crystal structure of the f7a mutant of the cytochrome c551 from pseudomonas aeruginosa (see paper)
43% identity, 66% coverage: 36:111/116 of query aligns to 6:80/82 of 2exvA

query
sites
2exvA

I

L

A

A

N

K

A

N

N

K

A

G

C
|
C

M

V

G

A

C
|
C

H
|
H

A

A

V

I

D

D

R

T

K

K

L

M

V

V

G
|
G

P
|
P

S

A

F

Y

Q

K

Q

D

I

V

A

A

R

K

Y

F

K

A

G

G

D

Q

A

A

Q

G

A

A

P

E

A

A

K

E

L

L

A

A

R

Q

K
x
R

V
x
I

K

K

D

N

G

G

G
x
S

S
x
Q

G
|
G

V
|
V

W
|
W

G
|
G

M

P

I
|
I

P
|
P

M
|
M

P

P

A

P

H
x
N

Q

-

S

A

M

V

S

S

D

D

A

D

D

E

I

A

R

Q

A

T

V

L

V

A

D

K

W

W

V

V

L

L

A

S

binding heme c: C12 (= C42), C15 (= C45), H16 (= H46), G24 (= G54), P25 (= P55), R47 (≠ K77), I48 (≠ V78), S52 (≠ G82), Q53 (≠ S83), G54 (= G84), V55 (= V85), W56 (= W86), G57 (= G87), I59 (= I89), P60 (= P90), M61 (= M91), N64 (≠ H94)

351cA Structure of cytochrome c551 from p. Aeruginosa refined at 1.6 angstroms resolution and comparison of the two redox forms (see paper)
46% identity, 60% coverage: 42:111/116 of query aligns to 12:80/82 of 351cA

query
sites
351cA

C
|
C

M

V

G

A

C
|
C

H
|
H

A

A

V

I

D

D

R

T

K

K

L

M

V

V

G
|
G

P
|
P

S

A

F
x
Y

Q

K

Q

D

I

V

A

A

R

K

Y

F

K

A

G

G

D

Q

A

A

Q

G

A

A

P

E

A

A

K

E

L
|
L

A

A

R

Q

K
x
R

V
x
I

K

K

D

N

G

G

G

S

S
x
Q

G
|
G

V
|
V

W
|
W

G
|
G

M

P

I
|
I

P
|
P

M
|
M

P

P

A

P

H

N

Q

-

S

A

M

V

S

S

D

D

A

D

D

E

I

A

R

Q

A

T

V

L

V

A

D

K

W

W

V

V

L

L

A

S

binding protoporphyrin ix containing fe: C12 (= C42), C15 (= C45), H16 (= H46), G24 (= G54), P25 (= P55), Y27 (≠ F57), L44 (= L74), R47 (≠ K77), I48 (≠ V78), Q53 (≠ S83), G54 (= G84), V55 (= V85), W56 (= W86), G57 (= G87), I59 (= I89), P60 (= P90), M61 (= M91)

5xecC Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
41% identity, 68% coverage: 33:111/116 of query aligns to 1:78/80 of 5xecC

query
sites
5xecC

G

N

Q

E

R

Q

I

L

A

A

N

K

A

Q

N

K

A

G

C
|
C

M

M

G

A

C
|
C

H
|
H

A

D

V

L

D

K

R

A

K

K

L

M

V

V

G

G

P
|
P

S

A

F
x
Y

Q

K

Q

D

I

V

A

A

R

K

Y

F

K

A

G

G

D

Q

A

A

Q

G

A

A

P

E

A

A

K

E

L
|
L

A

A

R

Q

K
x
R

V

I

K

K

D

N

G

G

G

S

S
x
Q

G

G

V
|
V

W
|
W

G
|
G

M

P

I
|
I

P

P

M
|
M

P

P

A

P

H
x
N

Q

-

S

A

M

V

S

S

D

D

A

D

D

E

I

A

R

Q

A

T

V

L

V

A

D

K

W

W

V

V

L

L

A

S

binding heme c: C10 (= C42), C13 (= C45), H14 (= H46), P23 (= P55), Y25 (≠ F57), L42 (= L74), R45 (≠ K77), Q51 (≠ S83), V53 (= V85), W54 (= W86), G55 (= G87), I57 (= I89), M59 (= M91), N62 (≠ H94)

5xecA Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
46% identity, 60% coverage: 42:111/116 of query aligns to 12:80/82 of 5xecA

query
sites
5xecA

C
|
C

M

V

G

A

C
|
C

H
|
H

A

A

V

I

D

D

R

T

K

K

L

K

V

V

G
|
G

P
|
P

S

A

F
x
Y

Q

A

Q

D

I

V

A

A

A

K

R

K

Y
|
Y

K

A

G

G

D

R

A

K

Q

D

A

A

P

V

A

D

K
x
Y

L

L

A

A

R

G

K

K

V
x
I

K

K

D

K

G

G

S
|
S

G
|
G

V
|
V

W
|
W

G
|
G

M

S

I
x
V

P

P

M
|
M

P

P

A

P

H

-

Q
|
Q

S

N

M

V

S

T

D

D

A

A

D

E

I

A

R

K

A

Q

V

L

V

A

D

Q

W

W

V
x
I

L

L

A

S

binding heme c: C12 (= C42), C15 (= C45), H16 (= H46), G24 (= G54), P25 (= P55), Y27 (≠ F57), Y34 (= Y64), Y43 (≠ K73), I48 (≠ V78), S53 (= S83), G54 (= G84), V55 (= V85), W56 (= W86), G57 (= G87), V59 (≠ I89), M61 (= M91), Q64 (= Q95), I78 (≠ V109)

1corA Investigation of the solution conformation of cytochromE C-551 from pseudomonas stutzeri (see paper)
38% identity, 68% coverage: 33:111/116 of query aligns to 3:80/82 of 1corA

query
sites
1corA

G

G

Q

E

R

A

I

L

A

F

N

K

A

S

N
x
K

A

P

C
|
C

M

A

G

A

C
|
C

H
|
H

A

S

V

I

D

D

R

A

K

K

L

L

V
|
V

G
|
G

P
|
P

S

A

F

F

Q

K

Q

E

I

V

A

A

R

K

Y

Y

K

A

G

G

D

Q

A

D

Q

G

A

A

P

A

A

D

K

L

L
|
L

A

A

R

G

K
x
H

V
x
I

K

K

D

N

G

G

G
x
S

S
x
Q

G
|
G

V
|
V

W
|
W

G
|
G

M

P

I
|
I

P

P

M
|
M

P

P

A

P

H
x
N

Q

-

S

P

M

V

S

T

D

E

A

E

D

E

I

A

R

K

A

I

V

L

V

A

D

E

W

W

V

I

L

L

A

S

binding heme c: K10 (≠ N40), C12 (= C42), C15 (= C45), H16 (= H46), V23 (= V53), G24 (= G54), P25 (= P55), L44 (= L74), H47 (≠ K77), I48 (≠ V78), S52 (≠ G82), Q53 (≠ S83), G54 (= G84), V55 (= V85), W56 (= W86), G57 (= G87), I59 (= I89), M61 (= M91), N64 (≠ H94)

6kq1A Crystal structure of cytochrome c551 from pseudomonas sp. Strain mt-1.
35% identity, 68% coverage: 33:111/116 of query aligns to 3:80/82 of 6kq1A

query
sites
6kq1A

G

G

Q
x
E

R

A

I

L

A

F

N

K

A

S

N
x
K

A

P

C
|
C

M

A

G

A

C
|
C

H
|
H

A

S

V

I

D

D

R

A

K

K

L

M

V

V

G
|
G

P
|
P

S

A

F

L

Q

K

Q

E

I
x
V

A

A

R

K

Y
|
Y

K

A

G

G

D

Q

A

E

Q

G

A

A

P

A

A

D

K

L

L
|
L

A

A

R

G

K
x
H

V
x
I

K

K

D

N

G

G

G

T

S
x
Q

G
|
G

V
x
N

W
|
W

G
|
G

M

P

I
|
I

P

P

M
|
M

P

P

A

P

H
x
N

Q

-

S

P

M

V

S

T

D

E

A

E

D
x
E

I

A

R

K

A

T

V

L

V

A

D

E

W

W

V

V

L

L

A

S

binding heme c: C12 (= C42), C15 (= C45), H16 (= H46), G24 (= G54), P25 (= P55), V30 (≠ I60), Y34 (= Y64), L44 (= L74), H47 (≠ K77), I48 (≠ V78), Q53 (≠ S83), G54 (= G84), N55 (≠ V85), W56 (= W86), G57 (= G87), I59 (= I89), M61 (= M91), N64 (≠ H94)
binding zinc ion: E4 (≠ Q34), K10 (≠ N40), E70 (≠ D101)

Sites not aligning to the query:

binding zinc ion: 2

1cchA The solution conformation of cytochromE C-551 from p.Stutzeri zobell determined by nmr+ (see paper)
37% identity, 68% coverage: 33:111/116 of query aligns to 3:80/82 of 1cchA

query
sites
1cchA

G

G

Q

E

R

A

I

L

A

F

N

K

A

S

N

K

A

P

C
|
C

M

A

G

A

C
|
C

H
|
H

A

S

V

V

D

D

R

T

K

K

L

M

V

V

G

G

P

P

S

A

F

L

Q

K

Q

E

I

V

A

A

R

K

Y

N

K

A

G

G

D

V

A

E

Q

G

A

A

P

A

A

D

K

T

L
|
L

A

A

R

L

K
x
H

V
x
I

K

K

D

N

G

G

G
x
S

S
x
Q

G

G

V
|
V

W
|
W

G
|
G

M

P

I
|
I

P

P

M
|
M

P
|
P

A

P

H
x
N

Q

-

S

P

M

V

S

T

D

E

A

E

D

E

I

A

R

K

A

I

V

L

V

A

D

E

W

W

V

V

L

L

A

S

binding heme c: C12 (= C42), C15 (= C45), H16 (= H46), L44 (= L74), H47 (≠ K77), I48 (≠ V78), S52 (≠ G82), Q53 (≠ S83), V55 (= V85), W56 (= W86), G57 (= G87), I59 (= I89), M61 (= M91), P62 (= P92), N64 (≠ H94)

5aurA Hydrogenobacter thermophilus cytochrome c552 dimer formed by domain swapping at n-terminal region (see paper)
40% identity, 67% coverage: 34:111/116 of query aligns to 2:81/83 of 5aurA

query
sites
5aurA

Q

E

R

Q

I

L

A

A

N

K

A

Q

N

K

A

G

C
|
C

M

M

G

A

C
|
C

H
|
H

A

D

V

L

D

K

R

A

-

G

K

K

L

K

V

V

G

G

P
|
P

S

A

F
x
Y

Q

A

Q

D

I

V

A

A

A

K

R

K

Y
|
Y

K

A

G

G

D

R

A

K

Q

D

A

A

P

V

A

D

K
x
Y

L

L

A

A

R

G

K

K

V
x
I

K

K

D

K

G

G

S
|
S

G
|
G

V
|
V

W
|
W

G
|
G

M

S

I
x
V

P
|
P

M
|
M

P

P

A

P

H

-

Q
|
Q

S

N

M

V

S

T

D

D

A

A

D

E

I

A

R

K

A

Q

V

L

V

A

D

Q

W

W

V

I

L

L

A

S

binding heme c: C10 (= C42), C13 (= C45), H14 (= H46), P26 (= P55), Y28 (≠ F57), Y35 (= Y64), Y44 (≠ K73), I49 (≠ V78), S54 (= S83), G55 (= G84), V56 (= V85), W57 (= W86), G58 (= G87), V60 (≠ I89), P61 (= P90), M62 (= M91), Q65 (= Q95)

4xxlA Crystal structure of class 1 cytochrome mtod from sideroxydans lithotrophicus es-1 (see paper)
33% identity, 73% coverage: 27:111/116 of query aligns to 1:89/92 of 4xxlA

query
sites
4xxlA

A

A

A

V

D

D

M

V

P

D

S

A

G

A

Q

K

R

S

I

L

A

A

N

R

A

E

N

N

A

N

C
|
C

M

F

G

K

C
|
C

H
|
H

A

G

V

V

D

D

R

K

K

E

L

K

V

D

G
|
G

P
|
P

S

S

F
x
Y

Q

K

I

V

A

A

A

E

R
x
K

Y
|
Y

K

R

G

G

D

K

A

A

Q

D

A

A

P

E

A

A

K

K

L

L

A

I

R

H

K
x
H

V

V

K

T

D

S

G

G

G

E

S

K

G
x
A

V
x
K

W

F

G

P

M

D

I

G

P

H

M

E

P

E

A

E

H
|
H

Q

K

S

N

M

I

-

N

-

G

-

K

-

A

S

S

D

P

A

E

D

A

I

I

R

K

A

N

V

L

V

V

D

D

W

W

V

I

L

L

A

S

binding heme c: C16 (= C42), C19 (= C45), H20 (= H46), G28 (= G54), P29 (= P55), Y31 (≠ F57), K37 (≠ R63), Y38 (= Y64), H51 (≠ K77), A58 (≠ G84), K59 (≠ V85), H68 (= H94)

Query Sequence

>H281DRAFT_02148 FitnessBrowser__Burk376:H281DRAFT_02148
MKLGVVGVVVASLMFGAASLFVSTAYAADMPSGQRIANANACMGCHAVDRKLVGPSFQQI
AARYKGDAQAPAKLARKVKDGGSGVWGMIPMPAHQSMSDADIRAVVDWVLAGAPSK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory