SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_02375 H281DRAFT_02375 amino acid/amide ABC transporter ATP-binding protein 1, HAAT family to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1g6hA Crystal structure of the adp conformation of mj1267, an atp- binding cassette of an abc transporter (see paper)
32% identity, 94% coverage: 7:249/259 of query aligns to 4:254/254 of 1g6hA

query
sites
1g6hA

I

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N

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Y

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P

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x
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G

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K

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x
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T
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T

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E

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binding adenosine-5'-diphosphate: F14 (= F17), F17 (= F20), N39 (= N42), G40 (= G43), S41 (≠ A44), G42 (= G45), K43 (= K46), S44 (≠ T47), T45 (= T48)

1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
32% identity, 93% coverage: 7:248/259 of query aligns to 4:253/253 of 1g9xB

query
sites
1g9xB

I

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N

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Y

F
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G

A
x
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x
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T

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I

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G

binding adenosine-5'-diphosphate: F14 (= F17), F17 (= F20), N39 (= N42), G40 (= G43), S41 (≠ A44), G42 (= G45), K43 (= K46), S44 (≠ T47), T45 (= T48)
binding magnesium ion: S44 (≠ T47), E176 (= E176)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
33% identity, 86% coverage: 15:236/259 of query aligns to 9:223/240 of 4ymuJ

query
sites
4ymuJ

R

K

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F

K

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G

S

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x
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A
x
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x
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x
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G

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x
S

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T

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C

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I

binding adenosine-5'-triphosphate: F11 (= F17), L14 (≠ F20), V16 (≠ A22), S36 (≠ N42), G37 (= G43), S38 (≠ A44), G39 (= G45), K40 (= K46), S41 (≠ T47), T42 (= T48), E51 (≠ T54), E162 (= E176), H194 (= H207)
binding magnesium ion: S41 (≠ T47), E162 (= E176)

1ji0A Crystal structure analysis of the abc transporter from thermotoga maritima
33% identity, 96% coverage: 2:250/259 of query aligns to 1:240/240 of 1ji0A

query
sites
1ji0A

I

M

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x
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x
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F

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x
E

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G

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D

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F

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A

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D

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V

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L

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E
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E

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A

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A

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K

K

V

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S

Q

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E

G

G

R

T

T

T

I

I

L

L

M

L

V

V

E

E

H

Q

N

N

M

A

N

L

V

G

I

A

A

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G

K

I

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S

A

D

H

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Y

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T

Y

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V

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L

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E

R

T

G

G

E

Q

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I

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A

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E

E

G

G

S

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Y

A

A

S

E

E

V

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S

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D

N

N

P

E

L

M

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A

binding adenosine-5'-triphosphate: Y16 (≠ F17), I19 (≠ F20), A21 (= A22), N41 (= N42), G42 (= G43), A43 (= A44), G44 (= G45), K45 (= K46), T46 (= T47), T47 (= T48), E88 (≠ Q89), D164 (= D175), E165 (= E176)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
30% identity, 90% coverage: 7:240/259 of query aligns to 2:227/241 of 4u00A

query
sites
4u00A

I

I

L

I

E

R

T

I

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R

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N

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F
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F

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x
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x
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I

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G

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x
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G

A
x
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K
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K

T
x
S

T
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T

C

L

F

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N

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T

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L

E

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D

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E

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D

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G

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I

V

T

V

V

F

L

M

Q

D

R

G

G

G

E

Q

V

I

L

V

A

E

E

E

G

G

S

R

Y

P

A

E

E

E

V

I

S

F

K

T

N

R

P

P

binding adenosine-5'-diphosphate: F12 (= F17), L15 (≠ F20), V17 (≠ A22), S37 (≠ N42), G38 (= G43), S39 (≠ A44), G40 (= G45), K41 (= K46), S42 (≠ T47), T43 (= T48)

3c4jA Abc protein artp in complex with atp-gamma-s
30% identity, 90% coverage: 7:240/259 of query aligns to 3:229/242 of 3c4jA

query
sites
3c4jA

I

M

L

I

E

D

T

V

R

H

G

Q

L

L

T

K

R

K

E

S

F
|
F

K

G

G

S

F

L

I

E

A
x
V

V

L

N

K

G

G

V

I

N

N

L

V

R

H

V

I

R

R

R

E

G

G

S

E

I

V

H

V

A

V

L

V

I

I

G

G

P
|
P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

T
x
S

T
|
T

-

F

-

L

-

R

C

C

F

L

N

N

L

L

T

E

K

D

F

F

L

D

E

E

P

-

T

-

A

-

G

G

Q

E

I

I

V

I

F

I

N

D

G

G

E

I

D

N

I

L

-

K

T

A

N

K

E

D

R

T

P

N

A

L

Q

N

I

K

A

V

R

R

R

E

G

E

I

V

I

G

R

M

S

V

F

F

Q

Q

I

R

S

F

A

N

V

L

F

F

P

P

H

H

L

M

T

T

A

V

L

L

Q

N

N

N

V

I

R

T

I

L

G

A

L

P

Q

M

R

K

S

-

L

-

G

-

T

-

A

-

F

V

H

R

F

K

W

W

K

P

S

R

E

E

R

K

T

A

L

-

R

-

Q

-

L

-

D

E

D

A

R

K

A

A

M

M

D

E

L

L

T

D

Q

K

V

V

G

G

L

L

T

K

D

D

F

K

A

A

D
x
H

V

A

L
x
Y

T

P

V

D

E

S

L

L

S

S

Y

G

G

G

R

Q

K

A

R

Q

A

R

L

V

E

A

I

I

A

A

T

R

T

A

L

L

A

A

M

M

E

E

P

P

E

K

L

I

M

M

L

L

L

F

D

D

E
|
E

P

P

T

T

Q

S

G

A

M

L

G

D

H

P

E

E

D

M

V

V

D

G

R

E

V

V

T

L

A

S

L

V

I

M

K

K

K

Q

V

L

S

A

S

N

-

E

G

G

R

M

T

T

I

M

L

V

M

V

V

V

E

T

H

H

N

E

M

M

N

G

V

F

I

A

A

R

G

E

I

V

S

G

D

D

T

R

I

V

T

L

V

F

L

M

Q

D

R

G

G

G

E

Y

V

I

L

I

A

E

E

E

G

G

S

K

Y

P

A

E

E

D

V

L

S

F

K

D

N

R

P

P

binding phosphothiophosphoric acid-adenylate ester: F13 (= F17), V18 (≠ A22), P37 (= P41), S38 (≠ N42), G39 (= G43), S40 (≠ A44), G41 (= G45), K42 (= K46), S43 (≠ T47), T44 (= T48), H133 (≠ D145), Y135 (≠ L147), E164 (= E176)

3c41J Abc protein artp in complex with amp-pnp/mg2+
30% identity, 90% coverage: 7:240/259 of query aligns to 3:229/242 of 3c41J

query
sites
3c41J

I

M

L

I

E

D

T

V

R

H

G

Q

L

L

T

K

R

K

E

S

F
|
F

K

G

G

S

F

L

I

E

A
x
V

V

L

N

K

G

G

V

I

N

N

L

V

R

H

V

I

R

R

R

E

G

G

S

E

I

V

H

V

A

V

L

V

I

I

G

G

P
|
P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

T
x
S

T
|
T

-

F

-

L

-
x
R

C

C

F

L

N

N

L

L

T

E

K

D

F

F

L

D

E

E

P

-

T

-

A

-

G

G

Q

E

I

I

V

I

F

I

N

D

G

G

E

I

D

N

I

L

-

K

T

A

N

K

E

D

R

T

P

N

A

L

Q

N

I

K

A

V

R

R

R

E

G

E

I

V

I

G

R

M

S

V

F

F

Q

Q

I

R

S

F

A

N

V

L

F

F

P

P

H

H

L

M

T

T

A

V

L

L

Q

N

N

N

V

I

R

T

I

L

G

A

L

P

Q

M

R

K

S

-

L

-

G

-

T

-

A

-

F

V

H

R

F

K

W

W

K

P

S

R

E

E

R

K

T

A

L

-

R

-

Q

-

L

-

D

E

D

A

R

K

A

A

M

M

D

E

L

L

T

D

Q

K

V

V

G

G

L

L

T

K

D

D

F

K

A

A

D
x
H

V

A

L
x
Y

T

P

V

D

E

S

L

L

S

S

Y

G

G

G

R

Q

K

A

R

Q

A

R

L

V

E

A

I

I

A

A

T

R

T

A

L

L

A

A

M

M

E

E

P

P

E

K

L

I

M

M

L

L

L

F

D
|
D

E

E

P

P

T

T

Q

S

G

A

M

L

G

D

H

P

E

E

D

M

V

V

D

G

R

E

V

V

T

L

A

S

L

V

I

M

K

K

K

Q

V

L

S

A

S

N

-

E

G

G

R

M

T

T

I

M

L

V

M

V

V

V

E

T

H

H

N

E

M

M

N

G

V

F

I

A

A

R

G

E

I

V

S

G

D

D

T

R

I

V

T

L

V

F

L

M

Q

D

R

G

G

G

E

Y

V

I

L

I

A

E

E

E

G

G

S

K

Y

P

A

E

E

D

V

L

S

F

K

D

N

R

P

P

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F17), V18 (≠ A22), P37 (= P41), S38 (≠ N42), G39 (= G43), S40 (≠ A44), G41 (= G45), K42 (= K46), S43 (≠ T47), T44 (= T48), R47 (vs. gap), H133 (≠ D145), Y135 (≠ L147)
binding magnesium ion: S43 (≠ T47), D163 (= D175)

2olkA Abc protein artp in complex with adp-beta-s
30% identity, 90% coverage: 7:240/259 of query aligns to 3:229/242 of 2olkA

query
sites
2olkA

I

M

L

I

E

D

T

V

R

H

G

Q

L

L

T

K

R

K

E

S

F
|
F

K

G

G

S

F
x
L

I

E

A
x
V

V

L

N

K

G

G

V

I

N

N

L

V

R

H

V

I

R

R

R

E

G

G

S

E

I

V

H

V

A

V

L

V

I

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

T
x
S

T
|
T

-

F

-

L

-

R

C

C

F

L

N

N

L

L

T

E

K

D

F

F

L

D

E

E

P

-

T

-

A

-

G

G

Q

E

I

I

V

I

F

I

N

D

G

G

E

I

D

N

I

L

-

K

T

A

N

K

E

D

R

T

P

N

A

L

Q

N

I

K

A

V

R

R

R

E

G

E

I

V

I

G

R

M

S

V

F

F

Q

Q

I

R

S

F

A

N

V

L

F

F

P

P

H

H

L

M

T

T

A

V

L

L

Q

N

N

N

V

I

R

T

I

L

G

A

L

P

Q

M

R

K

S

-

L

-

G

-

T

-

A

-

F

V

H

R

F

K

W

W

K

P

S

R

E

E

R

K

T

A

L

-

R

-

Q

-

L

-

D

E

D

A

R

K

A

A

M

M

D

E

L

L

T

D

Q

K

V

V

G

G

L

L

T

K

D

D

F

K

A

A

D

H

V

A

L

Y

T

P

V

D

E

S

L

L

S

S

Y

G

G

G

R

Q

K

A

R

Q

A

R

L

V

E

A

I

I

A

A

T

R

T

A

L

L

A

A

M

M

E

E

P

P

E

K

L

I

M

M

L

L

L

F

D

D

E

E

P

P

T

T

Q

S

G

A

M

L

G

D

H

P

E

E

D

M

V

V

D

G

R

E

V

V

T

L

A

S

L

V

I

M

K

K

K

Q

V

L

S

A

S

N

-

E

G

G

R

M

T

T

I

M

L

V

M

V

V

V

E

T

H

H

N

E

M

M

N

G

V

F

I

A

A

R

G

E

I

V

S

G

D

D

T

R

I

V

T

L

V

F

L

M

Q

D

R

G

G

G

E

Y

V

I

L

I

A

E

E

E

G

G

S

K

Y

P

A

E

E

D

V

L

S

F

K

D

N

R

P

P

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (= F17), L16 (≠ F20), V18 (≠ A22), S38 (≠ N42), G39 (= G43), S40 (≠ A44), G41 (= G45), K42 (= K46), S43 (≠ T47), T44 (= T48)

2oljA Abc protein artp in complex with adp/mg2+
30% identity, 90% coverage: 7:240/259 of query aligns to 3:229/242 of 2oljA

query
sites
2oljA

I

M

L

I

E

D

T

V

R

H

G

Q

L

L

T

K

R

K

E

S

F
|
F

K

G

G

S

F

L

I

E

A
x
V

V

L

N

K

G

G

V

I

N

N

L

V

R

H

V

I

R

R

R

E

G

G

S

E

I

V

H

V

A

V

L

V

I

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

T
x
S

T
|
T

-

F

-

L

-

R

C

C

F

L

N

N

L

L

T

E

K

D

F

F

L

D

E

E

P

-

T

-

A

-

G

G

Q

E

I

I

V

I

F

I

N

D

G

G

E

I

D

N

I

L

-

K

T

A

N

K

E

D

R

T

P

N

A

L

Q

N

I

K

A

V

R

R

R

E

G

E

I

V

I

G

R

M

S

V

F

F

Q

Q

I

R

S

F

A

N

V

L

F

F

P

P

H

H

L

M

T

T

A

V

L

L

Q

N

N

N

V

I

R

T

I

L

G

A

L

P

Q

M

R

K

S

-

L

-

G

-

T

-

A

-

F

V

H

R

F

K

W

W

K

P

S

R

E

E

R

K

T

A

L

-

R

-

Q

-

L

-

D

E

D

A

R

K

A

A

M

M

D

E

L

L

T

D

Q

K

V

V

G

G

L

L

T

K

D

D

F

K

A

A

D
x
H

V

A

L
x
Y

T

P

V

D

E

S

L

L

S

S

Y

G

G

G

R

Q

K

A

R

Q

A

R

L

V

E

A

I

I

A

A

T

R

T

A

L

L

A

A

M

M

E

E

P

P

E

K

L

I

M

M

L

L

L

F

D

D

E

E

P

P

T

T

Q

S

G

A

M

L

G

D

H

P

E

E

D

M

V

V

D

G

R

E

V

V

T

L

A

S

L

V

I

M

K

K

K

Q

V

L

S

A

S

N

-

E

G

G

R

M

T

T

I

M

L

V

M

V

V

V

E

T

H

H

N

E

M

M

N

G

V

F

I

A

A

R

G

E

I

V

S

G

D

D

T

R

I

V

T

L

V

F

L

M

Q

D

R

G

G

G

E

Y

V

I

L

I

A

E

E

E

G

G

S

K

Y

P

A

E

E

D

V

L

S

F

K

D

N

R

P

P

binding adenosine-5'-diphosphate: F13 (= F17), V18 (≠ A22), S38 (≠ N42), G39 (= G43), S40 (≠ A44), G41 (= G45), K42 (= K46), S43 (≠ T47), T44 (= T48), H133 (≠ D145), Y135 (≠ L147)

P04983 Ribose import ATP-binding protein RbsA; EC 7.5.2.7 from Escherichia coli (strain K12) (see paper)
30% identity, 92% coverage: 5:242/259 of query aligns to 2:234/501 of P04983

query
sites
P04983

D

E

T

A

I

L

L

L

E

Q

T

L

R

K

G

G

L

I

T

D

R

K

E

A

F

F

K

P

G

G

F

V

I

K

A

A

V

L

N

S

G

G

V

A

N

A

L

L

R

N

V

V

R

Y

R

P

G

G

S

R

I

V

H

M

A

A

L

L

I

V

G

G

P

E

N

N

G

G

A

A

G

G

K
|
K

T

S

T

T

C

M

F

M

N

K

L

V

L

L

T

T

K

G

F

I

L

Y

E

T

P

R

T

D

A

A

G

G

Q

T

I

L

V

L

F

W

N

L

G

G

E

K

D

E

I

T

T

T

N

F

E

T

R

G

P

P

A

K

Q

S

I

S

A

Q

R

E

R

A

G

G

I

I

I

G

R

I

S

I

F

H

Q

Q

I

E

S

L

A

N

V

L

F

I

P

P

H

Q

L

L

T

T

A

I

L

A

Q

E

N

N

V

I

R

F

I

L

G

G

L

-

Q

-

R

R

S

E

L

F

G

V

T

N

A

R

F

F

H

G

F

K

-

I

-

D

W

W

K

K

S

T

E

-

R

-

T

-

L

-

R

-

Q

-

L

M

D

Y

D

A

R

E

A

A

M

D

D

K

L

L

T

A

Q

K

V

L

G

N

L

L

T

R

D

F

F

K

A

S

D

D

V

K

L

L

T

V

V

G

E

D

L

L

S

S

Y

I

G

G

R

D

K

Q

R

Q

A

M

L

V

E

E

I

I

A

A

T

K

T

V

L

L

A

S

M

F

E

E

P

S

E

K

L

V

M

I

L

I

L

M

D

D

E

E

P

P

T

T

Q

D

G

A

M

L

G

T

H

D

E

T

D

E

V

T

D

E

R

S

V

L

T

F

A

R

L

V

I

I

K

R

K

E

V

L

S

K

S

S

-

Q

G

G

R

R

T

G

I

I

L

V

M

Y

V

I

E

S

H

H

N

R

M

M

N

K

V

E

I

I

A

F

G

E

I

I

S

C

D

D

T

D

I

V

T

T

V

V

L

F

Q

R

R

D

G

G

E

Q

V

F

L

I

A

A

E

E

G

R

S

E

Y

V

A

A

E

S

V

L

S

T

K

E

N

D

P

S

L

L

V

I

K43 (= K46) mutation to R: Loss of transport.

4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
33% identity, 90% coverage: 5:238/259 of query aligns to 2:225/285 of 4yerA

query
sites
4yerA

D

E

T

D

I

I

L

I

E

V

T

V

R

E

G

N

L

L

T

V

R

K

E

K

F
|
F

K

G

G

D

F
|
F

I

E

A
|
A

V

V

N

K

G

G

V

V

N

S

L

F

R

S

V

V

R

K

G

G

S

E

I

I

H

F

A

A

L

F

I

L

G

G

P
|
P

N
|
N

G
|
G

A
|
A

G
|
G

K
|
K

T
|
T

C

T

F

I

N

H

L

M

L

L

T

T

K

T

F

L

L

L

E

K

P

P

T

T

A

S

G

G

Q

K

I

A

V

W

F

V

N

A

G

G

E

H

D

D

I

V

T

L

N

K

E

E

R

-

P

P

A

R

Q

E

I

V

A

R

R

R

R

K

-

I

G

G

I

I

I

V

R

-

S

-

F

F

Q

Q

I

D

S

Q

A

S

V

L

F

D

P

R

H

E

L

L

T

T

A

A

L

Y

Q

E

N

N

V

M

R

Y

I

I

G

H

L

-

Q

-

R

-

S

-

L

-

G

G

T

K

A

I

F

Y

H

G

F

Y

W

G

K

G

S

E

E

-

R

-

T

-

L

-

R

-

Q

K

L

L

D

K

R

R

A

I

M

L

D

E

L

L

T

E

Q

F

V

V

G

E

L

L

T

L

D

E

F

F

A

K

D

D

V

K

L

P

T

V

V

K

E
x
T

L
x
F

S
|
S

Y

G

G

G

R

M

K

A

R

R

A

R

L

L

E

E

I

I

A

A

T

R

T

S

L

L

A

I

M

H

E

E

P

P

E

E

L

V

M

L

L

F

L

L

D

D

E

E

P

P

T

T

Q

I

G

G

M

L

G

D

H

P

E

H

D

T

V

R

D

A

R

H

V

M

T

W

A

E

L

Y

I

I

K

S

K

K

V

M

S

K

G

E

R

H

-

N

-

M

T

T

I

I

L

F

M

L

V

T

E

T

H

H

N

Y

M

M

N

D

V

E

I

A

A

E

G

Q

I

L

S

A

D

D

T

R

I

V

T

A

V

I

L

I

Q

D

R

H

G

G

E

K

V

I

L

I

A

A

E

L

G

G

S

T

Y

P

A

T

E

E

V

L

S

K

K

R

binding adenosine-5'-diphosphate: F14 (= F17), F17 (= F20), A19 (= A22), P38 (= P41), N39 (= N42), G40 (= G43), A41 (= A44), G42 (= G45), K43 (= K46), T44 (= T47), T45 (= T48), T135 (≠ E150), F136 (≠ L151), S137 (= S152)

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
30% identity, 85% coverage: 21:240/259 of query aligns to 40:254/382 of 7ahhC

query
sites
7ahhC

I
x
V

A
x
G

V

V

N

Y

G

D

V

T

N

N

L

F

R

E

V

I

R

N

R

E

G

G

S

E

I

I

H

F

A

V

L

I

I

M

G

G

P

L

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

T
x
S

T
|
T

C

L

F

L

N

R

L

L

T

N

K

R

F

L

L

I

E

E

P

P

T

T

A

S

G

G

Q

K

I

I

V

F

F

I

N

D

G

N

E

Q

D

D

I

V

-

A

-

T

-

L

T

N

N

K

E

E

R

D

P

L

A

L

Q

Q

I

V

A

R

R

R

R

K

G

T

I

M

I

S

R

M

S

V

F

F

Q

Q

I

N

S

F

A

G

V

L

F

F

P

P

H

H

L

R

T

T

A

I

L

L

Q

E

N

N

V

T

R

E

I

Y

G

G

L

L

Q

E

R

V

S

Q

L

-

G

-

T

-

A

-

F

-

H

N

F

V

W

P

K

K

S

E

E

E

R

R

T

-

L

-

R

R

Q

K

L

R

D

A

D

E

R

K

A

A

M

L

D

D

L

-

T

-

Q

N

V

A

G

N

L

L

T

L

D

D

F

F

A

K

D

D

V

Q

L

Y

T

P

V

K

E

Q

L

L

S

S

Y

G

G

G

R

M

K

Q

R

Q

A

R

L

V

E

G

I

L

A

A

T

R

T

A

L

L

A

A

M

N

E

D

P

P

E

E

L

I

M

L

L

L

L

M

D
|
D

E
|
E

P

A

T

F

Q

S

G

A

M

L

G

D

-

P

-

L

-

I

-

R

H

R

E

E

D

M

V

Q

D

D

R

E

V

L

T

L

A

E

L

L

I

Q

K

A

K

K

V

F

S

Q

S

-

G

-

R

K

T

T

I

I

L

I

M

F

V

V

E

S

H

H

N

D

M

L

N

N

V

E

I

A

A

L

G

R

I

I

S

G

D

D

T

R

I

I

T

A

V

I

L

M

Q

K

R

D

G

G

E

K

V

I

L

M

A

Q

E

I

G

G

S

T

Y

G

A

E

E

E

V

I

S

L

K

T

N

N

P

P

binding phosphoaminophosphonic acid-adenylate ester: V40 (≠ I21), G41 (≠ A22), S61 (≠ N42), G62 (= G43), S63 (≠ A44), G64 (= G45), K65 (= K46), S66 (≠ T47), T67 (= T48), D187 (= D175), E188 (= E176)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
30% identity, 85% coverage: 21:240/259 of query aligns to 40:254/382 of 7aheC

query
sites
7aheC

I

V

A

G

V

V

N

Y

G

D

V

T

N

N

L

F

R

E

V

I

R

N

R

E

G

G

S

E

I

I

H

F

A

V

L

I

I

M

G

G

P

L

N

S

G

G

A

S

G

G

K

K

T

S

T

T

C

L

F

L

N

R

L

L

T

N

K

R

F

L

L

I

E

E

P

P

T

T

A

S

G

G

Q

K

I

I

V

F

F

I

N

D

G

N

E

Q

D

D

I

V

-

A

-

T

-

L

T

N

N

K

E

E

R

D

P

L

A

L

Q

Q

I

V

A

R

R

R

R

K

G

T

I

M

I

S

R

M

S

V

F

F

Q

Q

I

N

S

F

A

G

V

L

F

F

P

P

H

H

L

R

T

T

A

I

L

L

Q

E

N

N

V

T

R

E

I

Y

G

G

L

L

Q

E

R

V

S

Q

L

-

G

-

T

-

A

-

F

-

H

N

F

V

W

P

K

K

S

E

E

E

R

R

T

-

L

-

R

R

Q

K

L

R

D

A

D

E

R

K

A

A

M

L

D

D

L

-

T

-

Q

N

V

A

G

N

L

L

T

L

D

D

F

F

A

K

D

D

V

Q

L

Y

T

P

V

K

E

Q

L

L

S

S

Y

G

G

G

R

M

K

Q

R

Q

A

R

L

V

E

G

I

L

A

A

T

R

T

A

L

L

A

A

M

N

E

D

P

P

E

E

L

I

M

L

L

L

L

M

D

D

E

E

P

A

T

F

Q

S

G

A

M

L

G

D

-

P

-

L

-

I

-

R

H

R

E

E

D

M

V

Q

D

D

R

E

V

L

T

L

A

E

L

L

I

Q

K

A

K

K

V

F

S

Q

S

-

G

-

R

K

T

T

I

I

L

I

M

F

V

V

E

S

H

H

N

D

M

L

N

N

V

E

I

A

A

L

G

R

I

I

S

G

D

D

T

R

I

I

T

A

V

I

L

M

Q

K

R

D

G

G

E

K

V

I

L

M

A

Q

E

I

G

G

S

T

Y

G

A

E

E

E

V

I

S

L

K

T

N

N

P

P

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 295, 296, 297, 298, 357, 372

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
31% identity, 85% coverage: 22:240/259 of query aligns to 20:232/343 of P30750

query
sites
P30750

A

A

V

L

N

N

G

N

V

V

N

S

L

L

R

H

V

V

R

P

R

A

G

G

S

Q

I

I

H

Y

A

G

L

V

I

I

G

G

P

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

T
x
S

T
|
T

-

L

-

I

-

R

C

C

F

V

N

N

L

L

T

E

K

R

F

-

L

-

E

-

P

P

T

T

A

E

G

G

Q

S

I

V

V

L

F

V

N

D

G

G

E

Q

D

E

I

L

T

T

N

T

E

L

R

S

P

E

A

S

Q

E

I

L

-

T

-

K

A

A

R

R

G

-

I

-

R

-

S

-

F

-

Q

Q

I

I

S

G

A

M

V

I

F

F

P

Q

H

H

L

F

T

N

A

L

L

L

Q

S

N

S

V

R

R

T

I

V

G

F

L

G

Q

N

R

V

S

A

L

L

G

P

T

L

A

E

F

L

H

D

F

N

W

T

K

P

S

K

E

D

R

E

T

V

L

K

R

R

Q

-

L

-

D

-

R

R

A

V

M

T

D

E

L

L

T

S

Q

L

V

V

G

G

L

L

T

G

D

D

F

K

A

H

D

D

V

S

L

Y

T

P

V

S

E

N

L

L

S

S

Y

G

G

G

R

Q

K

K

R

Q

A

R

L

V

E

A

I

I

A

A

T

R

T

A

L

L

A

A

M

S

E

N

P

P

E

K

L

V

M

L

L

L

L

C

D

D

E
|
E

P

A

T

T

Q

S

G

A

M

L

G

D

H

P

E

A

D

T

V

T

D

R

R

S

V

I

T

L

A

E

L

L

I

L

K

K

K

D

V

I

S

N

S

R

-

R

-

L

G

G

R

L

T

T

I

I

L

L

M

L

V

I

E

T

H

H

N

E

M

M

N

D

V

V

I

V

A

K

G

R

I

I

S

C

D

D

T

C

I

V

T

A

V

V

L

I

Q

S

R

N

G

G

E

E

V

L

L

I

A

E

E

Q

G

D

S

T

Y

V

A

S

E

E

V

V

S

F

K

S

N

H

P

P

40:46 (vs. 42:48, 71% identical) binding
E166 (= E176) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

6mjpB Lptb(e163q)fgc from vibrio cholerae (see paper)
29% identity, 93% coverage: 7:248/259 of query aligns to 2:235/240 of 6mjpB

query
sites
6mjpB

I

I

L

L

E

K

T

A

R

Q

G

H

L

L

T

A

R

K

E

S

F

Y

K

K

G

K

F

R

I

K

A

V

V

V

N

S

G

D

V

V

N

S

L

L

R

Q

V

V

R

E

R

S

G

G

S

Q

I

I

H

V

A

G

L

L

I

L

G

G

P

P

N

N

G

G

A

A

G

G

K

K

T

T

C

S

F

F

N

Y

L

M

L

I

T

V

K

G

F

L

L

V

E

A

P

R

T

D

A

E

G

G

Q

T

I

I

V

T

F

I

N

D

G

D

E

N

D

D

I

I

T

S

N

I

E

L

R

P

P

M

A

H

Q

S

I

R

A

S

R

R

R

M

G

G

I

I

I

G

R

Y

S

L

F

P

Q

Q

I

E

S

A

A

S

V

I

F

F

P
x
R

H
x
K

L

L

T

S

A

V

L

E

Q

D

N

N

V

I

R

M

I

A

G

V

L

L

Q

Q

R

-

S

-

L

-

G

-

T

-

A

-

F

-

H

-

F

-

W

T

K

R

S

E

E

E

R

L

T

T

L

H

R

E

Q

E

L

R

D

Q

D

D

R

K

A

L

M

E

D

D

L

L

T

E

Q

E

V

F

G

H

L

I

T

Q

D

H

F

I

A

R

D

K

V

S

L

A

T

G

V

M

E

A

L

L

S

S

Y

G

G

G

R

E

K

R

R

R

A

R

L

V

E

E

I

I

A

A

T

R

T

A

L

L

A

A

M

A

E

N

P

P

E

Q

L

F

M

I

L

L

D

D

E

Q

P

P

T

F

Q

A

G

G

M

V

G

D

H

P

E

I

D

S

V

V

D

I

R

D

V

I

T

K

A

K

L

I

I

I

K

E

K

H

V

L

-

R

S

D

S

R

G

G

R

L

T

G

I

V

L

L

M

I

V

T

E

D

H

H

N

N

M

V

N

R

V

E

I

T

A

L

G

D

I

V

S

C

D

E

T

K

I

A

T

Y

V

I

L

V

Q

S

R

Q

G

G

E

R

V

L

L

I

A

A

E

E

G

G

S

T

Y

P

A

Q

E

D

V

V

S

L

K

N

N

N

P

E

L

Q

V

V

M

K

Q

Q

A

V

Y

Y

M

L

G

G

binding 6-cyclohexylhexyl beta-D-glucopyranoside: R90 (≠ P95), K91 (≠ H96)

6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
29% identity, 93% coverage: 7:248/259 of query aligns to 2:235/240 of 6mjpA

query
sites
6mjpA

I

I

L

L

E

K

T

A

R

Q

G

H

L

L

T

A

R

K

E

S

F

Y

K

K

G

K

F

R

I

K

A

V

V

V

N

S

G

D

V

V

N

S

L

L

R

Q

V

V

R

E

R

S

G

G

S

Q

I

I

H

V

A

G

L

L

I

L

G

G

P

P

N

N

G

G

A

A

G

G

K

K

T

T

C

S

F

F

N

Y

L

M

L

I

T

V

K

G

F

L

L

V

E

A

P

R

T

D

A

E

G

G

Q

T

I

I

V

T

F

I

N

D

G

D

E

N

D

D

I

I

T

S

N

I

E

L

R

P

P

M

A

H

Q

S

I

R

A

S

R

R

R

M

G

G

I

I

I

G

R

Y

S

L

F

P

Q

Q

I

E

S

A

A

S

V

I

F

F

P

R

H

K

L

L

T

S

A

V

L

E

Q

D

N

N

V

I

R

M

I

A

G

V

L

L

Q

Q

R

-

S

-

L

-

G

-

T

-

A

-

F

-

H

-

F

-

W

T

K

R

S

E

E

E

R

L

T

T

L

H

R
x
E

Q

E

L

R

D

Q

D
|
D

R

K

A

L

M

E

D

D

L

L

T

E

Q

E

V

F

G

H

L

I

T

Q

D

H

F

I

A

R

D

K

V

S

L

A

T

G

V

M

E

A

L

L

S

S

Y

G

G

G

R

E

K

R

R

R

A

R

L

V

E

E

I

I

A

A

T

R

T

A

L

L

A

A

M

A

E

N

P

P

E

Q

L

F

M

I

L

L

D

D

E

Q

P

P

T

F

Q

A

G

G

M

V

G

D

H

P

E

I

D

S

V

V

D

I

R

D

V

I

T

K

A

K

L

I

I

I

K

E

K

H

V

L

-

R

S

D

S

R

G

G

R

L

T

G

I

V

L

L

M

I

V

T

E

D

H

H

N

N

M

V

N

R

V

E

I

T

A

L

G

D

I

V

S

C

D

E

T

K

I

A

T

Y

V

I

L

V

Q

S

R

Q

G

G

E

R

V

L

L

I

A

A

E

E

G

G

S

T

Y

P

A

Q

E

D

V

V

S

L

K

N

N

N

P

E

L

Q

V

V

M

K

Q

Q

A

V

Y

Y

M

L

G

G

binding calcium ion: E111 (≠ R125), D115 (= D129)

7ahdC Opua (e190q) occluded (see paper)
30% identity, 85% coverage: 21:240/259 of query aligns to 40:254/260 of 7ahdC

query
sites
7ahdC

I

V

A

G

V

V

N

Y

G

D

V

T

N

N

L

F

R

E

V

I

R

N

R

E

G

G

S

E

I

I

H

F

A

V

L

I

I

M

G

G

P

L

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

T
x
S

T
|
T

C

L

F

L

N

R

L

L

T

N

K

R

F

L

L

I

E

E

P

P

T

T

A

S

G

G

Q

K

I

I

V

F

F

I

N

D

G

N

E

Q

D

D

I

V

-

A

-

T

-

L

T

N

N

K

E

E

R

D

P

L

A

L

Q

Q

I

V

A

R

R

R

R

K

G

T

I

M

I

S

R

M

S

V

F

F

Q
|
Q

I

N

S

F

A

G

V

L

F

F

P

P

H

H

L

R

T

T

A

I

L

L

Q

E

N

N

V

T

R

E

I

Y

G

G

L

L

Q

E

R

V

S

Q

L

-

G

-

T

-

A

-

F

-

H

N

F

V

W

P

K

K

S

E

E

E

R

R

T

-

L

-

R

R

Q

K

L

R

D

A

D

E

R

K

A

A

M

L

D

D

L

-

T

-

Q

N

V

A

G

N

L

L

T

L

D

D

F

F

A

K

D

D

V

Q

L

Y

T

P

V
x
K

E
x
Q

L

L

S
|
S

Y

G

G
|
G

R
x
M

K

Q

R

Q

A

R

L

V

E

G

I

L

A

A

T

R

T

A

L

L

A

A

M

N

E

D

P

P

E

E

L

I

M

L

L

L

L

M

D

D

E
x
Q

P

A

T

F

Q

S

G

A

M

L

G

D

-

P

-

L

-

I

-

R

H

R

E

E

D

M

V

Q

D

D

R

E

V

L

T

L

A

E

L

L

I

Q

K

A

K

K

V

F

S

Q

S

-

G

-

R

K

T

T

I

I

L

I

M

F

V

V

E

S

H
|
H

N

D

M

L

N

N

V

E

I

A

A

L

G

R

I

I

S

G

D

D

T

R

I

I

T

A

V

I

L

M

Q

K

R

D

G

G

E

K

V

I

L

M

A

Q

E

I

G

G

S

T

Y

G

A

E

E

E

V

I

S

L

K

T

N

N

P

P

binding adenosine-5'-triphosphate: S61 (≠ N42), G62 (= G43), S63 (≠ A44), G64 (= G45), K65 (= K46), S66 (≠ T47), T67 (= T48), Q111 (= Q89), K161 (≠ V149), Q162 (≠ E150), S164 (= S152), G166 (= G154), M167 (≠ R155), Q188 (≠ E176), H221 (= H207)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 29, 39

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
31% identity, 85% coverage: 22:240/259 of query aligns to 21:233/344 of 6cvlD

query
sites
6cvlD

A

A

V

L

N

N

G

N

V

V

N

S

L

L

R

H

V

V

R

P

R

A

G

G

S

Q

I

I

H

Y

A

G

L

V

I

I

G

G

P

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

T
x
S

T
|
T

-

L

-

I

-

R

C

C

F

V

N

N

L

L

T

E

K

R

F

-

L

-

E

-

P

P

T

T

A

E

G

G

Q

S

I

V

V

L

F

V

N

D

G

G

E

Q

D

E

I

L

T

T

N

T

E

L

R

S

P

E

A

S

Q

E

I

L

-

T

-

K

A

A

R

R

G

-

I

-

R

-

S

-

F

-

Q

Q

I

I

S

G

A

M

V

I

F

F

P

Q

H

H

L

F

T

N

A

L

L

L

Q

S

N

S

V

R

R

T

I

V

G

F

L

G

Q

N

R

V

S

A

L

L

G

P

T

L

A

E

F

L

H

D

F

N

W

T

K

P

S

K

E

D

R

E

T

V

L

K

R

R

Q

-

L

-

D

-

R

R

A

V

M

T

D

E

L

L

T

S

Q

L

V

V

G

G

L

L

T

G

D

D

F

K

A

H

D

D

V

S

L

Y

T

P

V

S

E
x
N

L
|
L

S
|
S

Y
x
G

G
|
G

R
x
Q

K

K

R

Q

A

R

L

V

E

A

I

I

A

A

T

R

T

A

L

L

A

A

M

S

E

N

P

P

E

K

L

V

M

L

L

L

L

C

D

D

E

Q

P

A

T

T

Q

S

G

A

M

L

G

D

H

P

E

A

D

T

V

T

D

R

R

S

V

I

T

L

A

E

L

L

I

L

K

K

K

D

V

I

S

N

S

R

-

R

-

L

G

G

R

L

T

T

I

I

L

L

M

L

V

I

E

T

H
|
H

N

E

M

M

N

D

V

V

I

V

A

K

G

R

I

I

S

C

D

D

T

C

I

V

T

A

V

V

L

I

Q

S

R

N

G

G

E

E

V

L

L

I

A

E

E

Q

G

D

S

T

Y

V

A

S

E

E

V

V

S

F

K

S

N

H

P

P

binding phosphothiophosphoric acid-adenylate ester: S41 (≠ N42), G42 (= G43), A43 (= A44), G44 (= G45), K45 (= K46), S46 (≠ T47), T47 (= T48), N141 (≠ E150), L142 (= L151), S143 (= S152), G144 (≠ Y153), G145 (= G154), Q146 (≠ R155), H200 (= H207)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12, 14, 19

6mhzA Vanadate trapped cryo-em structure of e.Coli lptb2fg transporter (see paper)
30% identity, 93% coverage: 8:248/259 of query aligns to 3:235/235 of 6mhzA

query
sites
6mhzA

L

L

E

T

T

A

R

K

G

N

L

L

T

A

R

K

E

A

F
x
Y

K

K

G

G

F

R

I

R

A

V

V

V

N

E

G

D

V

V

N

S

L

L

R

T

V

V

R

N

R

S

G

G

S

E

I

I

H

V

A

G

L

L

I

L

G

G

P
|
P

N
|
N

G
|
G

A
|
A

G
|
G

K
|
K

T
|
T

C

T

F

F

N

Y

L

M

L

V

T

V

K

G

F

I

L

V

E

P

P

R

T

D

A

A

G

G

Q

N

I

I

V

I

F

I

N

D

G

D

E

D

D

D

I

I

T

S

N

L

E

L

R

P

P

L

A

H

Q

A

I

R

A

A

R

R

G

G

I

I

I

G

R

Y

S

L

F

P

Q
|
Q

I

E

S

A

A

S

V

I

F

F

P

R

H

R

L

L

T

S

A

V

L

Y

Q

D

N

N

V

L

R

M

I

A

G

V

L

L

Q

Q

R

-

S

-

L

-

G

-

T

-

A

-

F

-

H

-

F

-

W

I

K

R

S

D

E

D

R

L

T

S

L

A

R

E

Q

Q

L

R

D

E

D

D

R

R

A

A

M

N

D

E

L

L

L

M

T

E

Q

E

V

F

G

H

L

I

T

E

D

H

F

L

A

R

D

D

V

S

L

M

T

G

V

Q

E
x
S

L

L

S
|
S

Y
x
G

G
|
G

R
x
E

K

R

R

R

A

R

L

V

E

E

I

I

A

A

T

R

T

A

L

L

A

A

M

A

E

N

P

P

E

K

L

F

M

I

L

L

D

D