SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_02540 FitnessBrowser__Burk376:H281DRAFT_02540 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 12 hits to proteins with known functional sites (download)

O14289 3-isopropylmalate dehydratase; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
42% identity, 99% coverage: 3:199/200 of query aligns to 539:741/758 of O14289

query
sites
O14289

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Sites not aligning to the query:

486 modified: Phosphoserine
488 modified: Phosphoserine

P9WK95 3-isopropylmalate dehydratase small subunit; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
40% identity, 95% coverage: 1:190/200 of query aligns to 1:192/198 of P9WK95

query
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P9WK95

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K154 (≠ Q152) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Q58667 Methanogen homoaconitase small subunit; HACN; Homoaconitate hydratase; EC 4.2.1.114 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
35% identity, 61% coverage: 63:183/200 of query aligns to 54:167/170 of Q58667

query
sites
Q58667

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Sites not aligning to the query:

26 mutation R->K,V: Allows the recognition of substrates of IPMI enzymes since it becomes able to efficiently catalyze citraconate hydration and 3-isopropylmalate dehydration. Largely decreases substrate affinity for cis-homo(1-3)-aconitate.
27 T→A: Largely decreases substrate affinity for cis-homo(1-3)-aconitate while slightly increases activity on these substrates, and also decreases substrate affinity for maleate. Gains the ability to hydrate citraconate, an IPMI substrate.

2pkpA Crystal structure of 3-isopropylmalate dehydratase (leud)from methhanocaldococcus jannaschii dsm2661 (mj1271) (see paper)
35% identity, 61% coverage: 63:183/200 of query aligns to 54:167/167 of 2pkpA

query
sites
2pkpA

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binding zinc ion: C63 (= C72)

Sites not aligning to the query:

binding zinc ion: 13

D9X0I3 Aconitate hydratase A; ACN; Aconitase; EC 4.2.1.3 from Streptomyces viridochromogenes (strain DSM 40736 / JCM 4977 / BCRC 1201 / Tue 494) (see paper)
44% identity, 29% coverage: 63:120/200 of query aligns to 805:862/931 of D9X0I3

query
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D9X0I3

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Sites not aligning to the query:

125:129 mutation Missing: Retains 40% of aconitase activity. Improves RNA-binding ability.
538 C→A: Loss of aconitase activity. Cannot rescue the growth defect of a disruption mutant and results in only a slight increase in PTT production in the mutant. Shows weak IRE-binding activity.
763 R→E: Loss of aconitase activity and IRE-binding activity; when associated with E-767.
767 Q→E: Loss of aconitase activity and IRE-binding activity; when associated with E-763.

P09339 Aconitate hydratase A; ACN; Aconitase; Aconitate/2-methylaconitate hydratase; Iron-responsive protein-like; IRP-like; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.- from Bacillus subtilis (strain 168) (see 2 papers)
42% identity, 30% coverage: 63:121/200 of query aligns to 781:839/909 of P09339

query
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P09339

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
450 C→S: Loss of aconitase activity. It is glutamate auxotroph and accumulates citrate. Exhibits overexpression of the citB promoter and accumulates high levels of inactive aconitase.
741 R→E: Same aconitase activity compared to the wild-type. It is glutamate prototroph and accumulates citrate. Exhibits overexpression of the citB promoter and accumulates high levels of active aconitase.

P20004 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Bos taurus (Bovine) (see 2 papers)
31% identity, 51% coverage: 63:163/200 of query aligns to 658:764/780 of P20004

query
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P20004

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SR 670:671 (= SR 75:76) binding substrate

Sites not aligning to the query:

99 binding substrate
192:194 binding substrate
385 binding [4Fe-4S] cluster
448 binding [4Fe-4S] cluster
451 binding [4Fe-4S] cluster
474 binding substrate
479 binding substrate
607 binding substrate

8acnA Crystal structures of aconitase with isocitrate and nitroisocitrate bound (see paper)
31% identity, 51% coverage: 63:163/200 of query aligns to 630:736/753 of 8acnA

query
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8acnA

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active site: S641 (= S74), R643 (= R76)
binding nitroisocitric acid: S641 (= S74), S642 (= S75), R643 (= R76)

Sites not aligning to the query:

active site: 99, 100, 164, 446
binding nitroisocitric acid: 71, 74, 100, 164, 165, 446, 451, 579
binding iron/sulfur cluster: 100, 164, 166, 356, 357, 420, 423, 424

1fghA Complex with 4-hydroxy-trans-aconitate (see paper)
31% identity, 51% coverage: 63:163/200 of query aligns to 630:736/753 of 1fghA

query
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1fghA

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active site: S641 (= S74), R643 (= R76)
binding 4-hydroxy-aconitate ion: S641 (= S74), S642 (= S75), R643 (= R76)

Sites not aligning to the query:

active site: 99, 100, 164, 446
binding 4-hydroxy-aconitate ion: 71, 74, 100, 164, 165, 446, 451, 579
binding iron/sulfur cluster: 100, 164, 166, 356, 357, 420, 423, 424, 451

1amjA Steric and conformational features of the aconitase mechanism (see paper)
31% identity, 51% coverage: 63:163/200 of query aligns to 630:736/753 of 1amjA

query
sites
1amjA

I

V

L

V

I

I

A

G

G

D

A

E

N

N

F

Y

G

G

C

E

G

G

S
|
S

S

S

R
|
R

E

E

A

H

A

S

V

A

W

L

A

E

L

P

Q

R

G

F

L

L

G

G

L

G

R

R

V

A

V

I

I

I

A

T

P

K

S

S

F

F

G

A

E

R

I

I

F

H

M

E

S

T

N

N

C

L

F

K

Q

K

N

Q

G

G

V

L

L

L

P

P

V

L

V

T

L

F

P

A

E

D

-

P

-

A

-

D

-

Y

-

N

-

K

-

I

Q

H

Q

P

V

V

A

D

N

K

L

L

A

T

E

I

Q

Q

A

G

Q

L

S

K

G

D

A

F

A

A

V

P

T

G

A

K

D

P

L

L

D

-

T

T

C

C

V

I

I

I

T

K

A

H

P

P

D

N

G

G

T

T

S

Q

F

E

P

T

F

I

Q

L

V

L

D

N

P

H

V

T

R

F

R

N

E

E

M

T

L

Q

I

I

E

E

active site: S641 (= S74), R643 (= R76)
binding sulfate ion: R643 (= R76)

Sites not aligning to the query:

active site: 99, 100, 164, 446
binding iron/sulfur cluster: 144, 166, 357, 420, 423
binding sulfate ion: 71, 579

1amiA Steric and conformational features of the aconitase mechanism (see paper)
31% identity, 51% coverage: 63:163/200 of query aligns to 630:736/753 of 1amiA

query
sites
1amiA

I

V

L

V

I

I

A

G

G

D

A

E

N

N

F

Y

G

G

C

E

G

G

S
|
S

R
|
R

E

E

A

H

A

S

V

A

W

L

A

E

L

P

Q

R

G

F

L

L

G

G

L

G

R

R

V

A

V

I

I

I

A

T

P

K

S

S

F

F

G

A

E

R

I

I

F

H

M

E

S

T

N

N

C

L

F

K

Q

K

N

Q

G

G

V

L

L

L

P

P

V

L

V

T

L

F

P

A

E

D

-

P

-

A

-

D

-

Y

-

N

-

K

-

I

Q

H

Q

P

V

V

A

D

N

K

L

L

A

T

E

I

Q

Q

A

G

Q

L

S

K

G

D

A

F

A

A

V

P

T

G

A

K

D

P

L

L

D

-

T

T

C

C

V

I

I

I

T

K

A

H

P

P

D

N

G

G

T

T

S

Q

F

E

P

T

F

I

Q

L

V

L

D

N

P

H

V

T

R

F

R

N

E

E

M

T

L

Q

I

I

E

E

active site: S641 (= S74), R643 (= R76)
binding alpha-methylisocitric acid: S641 (= S74), S642 (= S75), R643 (= R76)

Sites not aligning to the query:

active site: 99, 100, 164, 446
binding alpha-methylisocitric acid: 71, 74, 100, 164, 165, 446, 451, 579
binding iron/sulfur cluster: 100, 144, 164, 166, 356, 357, 420, 423, 445

1acoA Crystal structure of aconitase with transaconitate bound (see paper)
31% identity, 51% coverage: 63:163/200 of query aligns to 630:736/753 of 1acoA

query
sites
1acoA

I

V

L

V

I

I

A

G

G

D

A

E

N

N

F

Y

G

G

C

E

G

G

S
|
S

R
|
R

E

E

A

H

A

S

V

A

W

L

A

E

L

P

Q

R

G

F

L

L

G

G

L

G

R

R

V

A

V

I

I

I

A

T

P

K

S

S

F

F

G

A

E

R

I

I

F

H

M

E

S

T

N

N

C

L

F

K

Q

K

N

Q

G

G

V

L

L

L

P

P

V

L

V

T

L

F

P

A

E

D

-

P

-

A

-

D

-

Y

-

N

-

K

-

I

Q

H

Q

P

V

V

A

D

N

K

L

L

A

T

E

I

Q

Q

A

G

Q

L

S

K

G

D

A

F

A

A

V

P

T

G

A

K

D

P

L

L

D

-

T

T

C

C

V

I

I

I

T

K

A

H

P

P

D

N

G

G

T

T

S

Q

F

E

P

T

F

I

Q

L

V

L

D

N

P

H

V

T

R

F

R

N

E

E

M

T

L

Q

I

I

E

E

active site: S641 (= S74), R643 (= R76)
binding aconitate ion: S641 (= S74), S642 (= S75), R643 (= R76)

Sites not aligning to the query:

active site: 99, 100, 164, 446
binding iron/sulfur cluster: 100, 144, 164, 166, 356, 357, 420, 423, 445
binding aconitate ion: 71, 164, 165, 446, 451, 579

Query Sequence

>H281DRAFT_02540 FitnessBrowser__Burk376:H281DRAFT_02540
MERFQTVSGPAAPLLRRDVDTDIIIRIERLTGLPKDQLGQYAFEVWRGPDFVLDREPFVG
APILIAGANFGCGSSREAAVWALQGLGLRVVIAPSFGEIFMSNCFQNGVLPVVLPEQQVA
NLAEQAQSGAAVTADLDTCVITAPDGTSFPFQVDPVRREMLIEGADEMSLTLRRQDLIRQ
WQADDARRRPWVYVGSRSAD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory