SitesBLAST

Q9X0I0 Adenylosuccinate lyase; ASL; Adenylosuccinase; ASase; EC 4.3.2.2 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
27% identity, 94% coverage: 20:448/456 of query aligns to 3:427/431 of Q9X0I0

query
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H141 (= H161) active site, Proton donor/acceptor

P12047 Adenylosuccinate lyase; ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor; EC 4.3.2.2 from Bacillus subtilis (strain 168) (see paper)
29% identity, 90% coverage: 20:429/456 of query aligns to 3:408/431 of P12047

query
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P12047

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H89 (= H109) mutation to Q: Abolishes enzyme activity.
H141 (= H161) mutation to Q: Abolishes enzyme activity.
Q212 (≠ W234) mutation to E: Decreases catalytic activity 1000-fold.; mutation to M: Abolishes enzyme activity.
N270 (= N292) mutation N->D,L: Abolishes enzyme activity.
R301 (= R323) mutation R->K,Q: Abolishes enzyme activity.

P30566 Adenylosuccinate lyase; ADSL; ASL; Adenylosuccinase; ASase; EC 4.3.2.2 from Homo sapiens (Human) (see 13 papers)
27% identity, 94% coverage: 22:448/456 of query aligns to 21:459/484 of P30566

query
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P30566

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Y

F

L
x
T

G

G

L
x
R

A

A

P

S

Q

Q

M

Q

V

V

D

Q

R

R

M26 (= M27) to L: in ADSLD; severe; dbSNP:rs1311171245
I72 (= I74) to V: in ADSLD; severe
P100 (≠ G102) to A: in ADSLD; moderate; dbSNP:rs119450942
Y114 (≠ D116) to H: in ADSLD; severe; total loss of activity; dbSNP:rs374259530
R141 (≠ I143) to W: in ADSLD; severe; dbSNP:rs756210458
H159 (= H161) active site, Proton donor/acceptor
R190 (= R192) to Q: in ADSLD; moderate; dbSNP:rs28941471
R194 (≠ A196) to C: in ADSLD; severe; reduces protein stability; dbSNP:rs1465152683
K246 (≠ D239) to E: in ADSLD; moderate; strongly reduced catalytic activity; dbSNP:rs119450944
D268 (= D261) to N: in ADSLD; severe; total loss of activity; dbSNP:rs746501563
S289 (= S284) active site, Proton donor/acceptor
R303 (≠ L298) to C: in ADSLD; mild; strongly reduced activity with SAMP, but only slightly reduced activity with SAICAR; abolishes cooperativity; dbSNP:rs373458753
L311 (≠ Q309) to V: in ADSLD; severe; slightly reduced enzyme activity
P318 (vs. gap) to L: in ADSLD; severe; dbSNP:rs202064195
V364 (= V358) to M: in ADSLD; severe; dbSNP:rs370851726
R374 (≠ G368) to W: in ADSLD; severe; dbSNP:rs376533026
S395 (vs. gap) to R: in ADSLD; severe
R396 (= R389) to C: in ADSLD; severe; abolishes cooperativity and reduces enzyme activity; dbSNP:rs755492501; to H: in ADSLD; severe; abolishes cooperativity and reduces enzyme activity; dbSNP:rs763542069
D422 (≠ A413) to Y: in ADSLD; moderate; dbSNP:rs119450943
L423 (= L414) to V: in ADSLD; moderate
R426 (≠ L417) to H: in ADSLD; severe; most frequent mutation; dbSNP:rs119450941
D430 (≠ T421) to N: in ADSLD; mild; dbSNP:rs554254383
S438 (≠ D427) to P: in ADSLD; severe; dbSNP:rs119450940
S447 (≠ A436) to P: in ADSLD; severe; dbSNP:rs777821034
T450 (≠ L439) to S: in ADSLD; moderate; dbSNP:rs372895468
R452 (≠ L441) to P: in ADSLD; severe

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine; A → V: in ADSLD; severe; dbSNP:rs143083947
3 A → V: in ADSLD; severe

5nx9D Crystal structure of neanderthal adenylosuccinate lyase (adsl) in complex with its products amp and fumarate (see paper)
26% identity, 94% coverage: 22:448/456 of query aligns to 14:452/477 of 5nx9D

query
sites
5nx9D

F
x
Y

G

A

T

S

A

P

K

E

M

M

R

C

A

F

L

V

F

F

S

S

D

D

Y

R

A

Y

L

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F

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R

R

T

Y

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I

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D

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V

L

E

W

V

L

A

W

L

L

A

A

K

E

A

A

E

E

A

Q

R

T

V

L

G

G

V

L

I

P

P

I

T

T

D

D

A

E

A

-

D

Q

V

I

I

Q

A

E

R

M

E

K

S

S

Q

N

I

L

E

E

R

N

I

I

D

D

F

F

D

K

H

M

M

A

R

A

E

E

T

E

D

K

I

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L

G
x
R

Y
x
H

P
x
D

I

V

L

M

P

A

L

H

V

V

H

H

Q

T

L

F

V

G

G

H

M

C

C

C

G

P

E

K

A

A

G

A

R

G

Y

I

V

I

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H

W

L

G

G

A

A

T

T

T
x
S

Q

C

D

Y

I

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M

G

D

D

T

N

A

T

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D

A

L

L

I

Q

I

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R

R

D

N

A

A

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D

S

L

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L

D

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E

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R

R

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S

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P

M

T

A

L

G

G

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F

T
|
T

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|
H

L

F

Q

Q

Q

P

A

A

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Q

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T

T

T

F

V

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G

Y

K

K

R

T

C

A

C

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W

W

L

I

A

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M

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F

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D

C

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D

Q

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N

I

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R

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P

D

R

D

V

L

A

R

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F

V

R

E

G

F

V

A

K

G

G

A

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G

G

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T

L

Q

A

A

S

S

L

F

-

L

-

Q

-

L

-

F

-

E

G

G

D

D

-

D

-

H

-

K

-

V

K

E

G

Q

F

L

D

D

V

K

Q

M

K

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A

T

L

E

A

K

A

A

E

G

L

F

Q

K

L

R

G

A

V

F

P

I

A

I

T

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T

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W
x
Q

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Y

A

T

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R

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K

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F

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A

I

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A

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A

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K

I

I

A

C

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D

I

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I

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M

L

A

A

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N

N

-

E

-

F

L

G

K

E

E

V

M

Y

E

E

E

P

P

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F

V

E

K

K

G

Q

R

Q

G

I

A

G

S
|
S

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A

M

M

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P

Q

Y

K
|
K

R

R

N

N

P

P

I

M

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E
|
E

L
x
R

M

C

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C

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S

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-

A

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-

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Q

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A

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x
L

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x
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x
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V

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A

M

M

A

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Y

A

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G

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-

S

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Q

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A

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I

K

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I

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-

D

-

A

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-

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R

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A

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N

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L

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Q

R

A

D

D

A

A

-

Y

-

F

-

S

-

P

-

I

-

H

-

S

-

Q

I

L

N

D

A

H

M

L

T

L

D

D

P

P

A

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N

S

Y

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V

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Q

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active site: H79 (≠ Y88), T151 (= T160), H152 (= H161), S283 (= S285), K288 (= K290), E295 (= E297)
binding 2-[9-(3,4-dihydroxy-5-phosphonooxymethyl-tetrahydro-furan-2-yl)-9h-purin-6-ylamino]-succinic acid: T151 (= T160), H152 (= H161)
binding adenosine monophosphate: Y14 (≠ F22), R78 (≠ G87), H79 (≠ Y88), D80 (≠ P89), S105 (≠ T114), Q234 (≠ W234), R296 (≠ L298), L324 (≠ T325), S327 (≠ W328), A328 (≠ H329), R331 (≠ W332)
binding fumaric acid: H79 (≠ Y88), S105 (≠ T114), Q234 (≠ W234), S282 (= S284), S283 (= S285), K288 (= K290)

Sites not aligning to the query:

binding adenosine monophosphate: 13

Q05911 Adenylosuccinate lyase; ASL; Adenylosuccinase; ASase; EC 4.3.2.2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
25% identity, 98% coverage: 5:450/456 of query aligns to 8:458/482 of Q05911

query
sites
Q05911

T

T

P

P

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L

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I

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I

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A

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I

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M

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C

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P

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A

A

A

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G

Y

I

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I

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H

W

L

G

G

A

A

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A

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A

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x
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K196 (≠ A201) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

5hw2A Crystal structure of adenylosuccinate lyase from francisella tularensis complexed with fumaric acid
26% identity, 75% coverage: 22:361/456 of query aligns to 5:327/419 of 5hw2A

query
sites
5hw2A

F

Y

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|
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|
H

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A

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A

A

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-

A

A

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G

G

V

L

P

P

-

V

-

E

A

V

T

S

T

T

W

Q

H

V

V

I

A

P

R

R

D

D

G

R

F

I

A

A

E

K

A

L

V

I

N

S

L

I

L

H

A

G

L

L

V

I

T

A

G

S

S

A

L

I

G

E

K

R

I

L

A

A

T

V

D

E

I

I

M

R

I

H

M

L

A

H

S

R

N

S

E

D

F

V

G

F

E

E

V

V

Y

Y

E

E

P

G

F

F

V

-

K

-

G

-

R

-

G

-

A

-

S

-

T

-

M

-

P

-

Q

-

K
|
K

R

K

N

N

P

P

I

I

S

S

S

T

E
|
E

L

N

M

L

L

T

A

G

A

M

A

A

K

R

A

M

V

L

R

R

Q

S

Q

H

A

V

G

S

L

I

M

A

V

L

D

E

A

N

M

C

I

V

Q

L

D

W

F

H

E

E

R

R

A

D

T

I

G

S

P

H

W

S

H

S

A

A

E

E

W

R

I

F

A

Y

I

L

P

P

E

D

S

N

F

F

I

G

L

I

A

M

S

V

G

Y

A

A

L

L

H

R

Q

R

A

M

K

K

F

N

A

T

L

I

G

D

G

N

L

L

I

V

V

V

D

Q

T

R

D

D

active site: H67 (≠ T83), S140 (≠ T160), H141 (= H161), K256 (= K290), E263 (= E297)
binding fumaric acid: H67 (≠ T83), H89 (= H109), T93 (= T113), S94 (≠ T114)

4eeiB Crystal structure of adenylosuccinate lyase from francisella tularensis complexed with amp and succinate
26% identity, 75% coverage: 22:361/456 of query aligns to 5:327/423 of 4eeiB

query
sites
4eeiB

F

Y

G

D

T

V

A

A

K

E

M

I

R

S

A

K

L

I

F

W

S

A

D

D

Y

E

A

N

L

K

V

Y

Q

A

R

K

Y

M

I

L

D

E

V

V

E

E

V

L

A

A

L

I

A

L

K

E

A

A

E

L

A

E

R

D

V

R

G

M

V

V

I

P

P

K

T

G

D

T

A

A

D

E

V

I

I

R

A

A

R

R

E

-

S

A

Q

Q

I

I

-

R

-

P

E

E

R

R

I

V

D

D

F

E

D

I

H

E

M

K

R

V

E

T

E
x
K

T
x
H

D
|
D

I

I

V

I

G

A

Y

F

P

-

I

C

L

T

P

S

L

I

V

A

H

E

Q

Q

L

F

V

T

G

A

M

-

C

-

G

-

E

E

A

T

G

G

R

K

Y

F

V

F

H

H

W

F

G

G

A

V

T

T

T

S

Q

S

D

D

I

I

M

I

D

D

T

S

A

A

V

L

A

S

L

L

Q

Q

V

I

R

R

D

D

A

S

L

M

D

S

S

Y

I

V

D

I

A

K

D

D

I

L

R

E

E

A

L

L

R

C

G

D

I

S

L

L

V

L

D

T

L

K

S

A

K

E

H

T

R

K

D

E

T

I

P

I

M

T

A

M

G

G

R

R

T
x
S

H
|
H

L

G

Q

M

Q

F

A

A

L

E

P

P

V

M

T

S

F

F

G

G

Y

Q

K

K

T

F

A

L

I

G

W

A

L

Y

A

V

M

E

F

F

D

K

R

R

H

R

Q

L

Q

K

R

D

I

L

A

K

Q

D

L

F

R

Q

P

K

R

D

V

G

A

L

V

T

V

V

E

Q

F

F

A

S

G

G

A

A

A

V

G

G

T

N

L

Y

A

C

S

I

L

L

G

T

D

T

K

E

G

-

F

-

D

D

V

E

Q

K

K

K

A

-

L

-

A

A

E

D

L

I

Q

-

L

L

G

G

V

L

P

P

-

V

-

E

A

V

T

S

T

T

W
x
Q

H

V

V

I

A

P

R

R

D

D

G

R

F

I

A

A

E

K

A

L

V

I

N

S

L

I

L

H

A

G

L

L

V

I

T

A

G

S

S

A

L

I

G

E

K

R

I

L

A

A

T

V

D

E

I

I

M

R

I

H

M

L

A

H

S

R

N

S

E

D

F

V

G

F

E

E

V

V

Y

Y

E

E

P

G

F

F

V

-

K

-

G

-

R

-

G

-

A

-

S

-

T

-

M

-

P

-

Q

-

K
|
K

R

K

N

N

P

P

I

I

S

S

S

T

E
|
E

L

N

M

L

L

T

A

G

A

M

A

A

K

R

A

M

V

L

R

R

Q

S

Q

H

A

V

G

S

L

I

M

A

V

L

D

E

A

N

M

C

I

V

Q

L

D

W

F

H

E

E

R
|
R

A

D

T
x
I

G

S

P

H

W
x
S

H

S

A

A

E

E

W
x
R

I

F

A

Y

I

L

P

P

E

D

S

N

F

F

I

G

L

I

A

M

S

V

G

Y

A

A

L

L

H

R

Q

R

A

M

K

K

F

N

A

T

L

I

G

D

G

N

L

L

I

V

V

V

D

Q

T

R

D

D

active site: H67 (≠ T83), S140 (≠ T160), H141 (= H161), K256 (= K290), E263 (= E297)
binding adenosine monophosphate: K66 (≠ E82), H67 (≠ T83), D68 (= D84), Q212 (≠ W234), R289 (= R323), I291 (≠ T325), S294 (≠ W328), R298 (≠ W332)

5vkwB Crystal structure of adenylosuccinate lyase ade13 from candida albicans
25% identity, 98% coverage: 5:450/456 of query aligns to 8:451/469 of 5vkwB

query
sites
5vkwB

T

S

T

T

P

P

L

L

S

S

T

R

V

-

F

-

D

-

S

-

I

-

L

-

F

-

R

-

D

-

A

-

F

Y

G

A

T

S

A

E

K

E

M

M

R

S

A

K

L

I

F

F

S

S

-

L

-

R

D

N

Y

R

A

F

L

S

V

T

Q

W

R

R

Y

K

I

L

D

W

V

L

E

N

V

L

A

A

L

I

A

A

K

E

A

K

E

E

A

V

R

G

V

L

G

S

V

V

I

I

P

T

T
x
D

D

E

A

A

A

I

D
x
E

V

Q

I

M

A

K

R

Q

E

H

S

L

Q

E

I

I

E

T

R

D

I

K

D

E

F

I

D

Q

H

D

M

A

R

A

E

V

E

E

T

E

D

A

I

K

V

V

G

R

Y
x
H

P

D

I

V

L

M

P

A

L

H

V

V

H

H

Q

V

L

F

V

G

G

E

M

T

C

C

G

P

E

S

A

A

G

A

R

G

Y

I

V

I

H

H

W

L

G

G

A

A

T

T

T

S

Q

C

D

F

I

V

M

T

D

D

T

N

A

A

V

D

A

L

L

I

Q

F

V

L

R

R

D

D

A

A

L

Y

D

D

S

V

I

L

D

-

A

-

D

-

I

I

R

P

E

K

L

L

R

V

G

N

I

V

L

I

V

D

D

R

L

L

S

S

K

K

-

F

-

A

-

L

K

E

H

Y

R

K

D

D

T

L

P

P

M

V

A

L

G

G

R

W

T
|
T

H
|
H

L

F

Q

Q

Q

P

A

A

L

Q

P

L

V

T

T

T

F

V

G

G

Y

K

K

R

T

A

A

T

I

L

W

W

L

L

A

Q

M

E

F

L

D

L

R

W

H

D

Q

L

Q

R

R

N

I

M

A

V

Q

R

L

A

R

R

P

N

R

D

V

I

A

G

V

L

V

R

E

G

F

V

A

K

G

G

A

T

G

G

T

T

L

Q

A

A

S

S

L

F

-

L

-

S

-

L

-

F

-

H

-

G

-

D

G

H

D

D

K

K

G

V

F

E

D

E

V

L

Q

D

K

K

A

R

L

V

A

V

A

E

E

L

L

L

Q

G

L

F

G

D

V

I

-

V

-

Y

P

P

A

V

T

T

T

G

W

Q

H

T

V

Y

A

S

R

R

D

K

G

I

F

D

A

I

E

D

A

V

V

L

N

S

L

P

L

L

A

A

L

S

V

F

T

G

G

A

S

T

L

A

G

H

K

K

I

F

A

A

T

T

D

D

I

I

M

R

I

L

M

L

A

A

S

N

N

-

E

-

F

L

G

K

E

E

V

I

Y

E

E

E

P

P

F

F

V

E

K

K

G

-

R

-

G

-

A

-

S

-

T

-

M

M

P

A

Q

Y

K
|
K

R

R

N

N

P

P

I

M

S

R

S

C

E
|
E

L

R

M

V

L

C

A

S

A

L

A

A

K

-

A

-

V

-

R

R

Q

H

Q

L

A

G

G

G

L

L

M

F

V

N

D

D

A

A

M

V

-

Q

-

T

-

A

-

S

I

V

Q

Q

D

W

F

F

E

E

R

R

A

T

T

L

G

D

P

D

W

S

H

A

A

I

E

R

W

R

I

I

A

S

I

L

P

P

E

S

S

A

F

F

I

L

L

T

A

V

S

D

G

I

A

L

L

L

H

S

Q

T

A

M

K

L

F

N

A

I

L

T

G

S

G

G

L

L

I

V

V

V

D

Y

T

P

D

K

R

V

M

I

K

E

H

R

N

R

L

I

G

N

I

S

S

E

K

L

G

P

L

F

I

M

V

A

A

T

E

E

A

N

V

I

M

I

M

M

Q

A

M

M

A

V

P

E

Y

K

T

G

G

G

-

S

R

R

Q

Q

Q

D

A

C

H

H

D

E

-

E

-

I

-

R

-

V

I

L

V

S

Y

H

D

Q

A

A

C

S

R

A

T

V

V

V

N

K

E

Q

S

E

G

G

-

D

-

N

T

D

L

L

A

I

E

E

A

R

L

I

A

K

A

S

L

-

P

-

E

-

V

-

T

T

R

E

H

Y

F

F

D

K

-

P

-

I

R

W

D
x
N

A

D

I

L

N
x
D

A

T

M

L

T

L

D

D

P

P

A

K

N

T

Y

F

L

V

G

G

L

R

A

A

P

P

Q

Q

M

Q

V

T

D

E

R

K

A

F

I

V

active site: H83 (≠ Y88), T155 (= T160), H156 (= H161), K285 (= K290), E292 (= E297)
binding calcium ion: D54 (≠ T59), E58 (≠ D63)
binding magnesium ion: N428 (≠ D427), D431 (≠ N430)

5nxaA Crystal structure of neanderthal adenylosuccinate lyase (adsl)in complex with its products aicar and fumarate (see paper)
24% identity, 94% coverage: 22:448/456 of query aligns to 14:439/464 of 5nxaA

query
sites
5nxaA

F
x
Y

G

A

T

S

A

P

K

E

M

M

R

C

A

F

L

V

F

F

S

S

D

D

Y

R

A

Y

L

K

V

F

Q

R

R

T

Y

W

I

R

D

Q

V

L

E

W

V

L

A

W

L

L

A

A

K

E

A

A

E

E

A

Q

R

T

V

L

G

G

V

L

I

P

P

I

T

T

D

D

A

E

A

-

D

Q

V

I

I

Q

A

E

R

M

E

K

S

S

Q

N

I

L

E

E

R

N

I

I

D

D

F

F

D

K

H

M

M

A

R

A

E

E

T

E

D

K

I

R

V

L

G
x
R

Y
x
H

P
x
D

I

V

L

M

P

A

L

H

V

V

H

H

Q

T

L

F

V

G

G

H

M

C

C

C

G

P

E

K

A

A

G

A

R

G

Y

I

V

I

H

H

W

L

G

G

A

A

T
|
T

T
x
S

Q

C

D

Y

I

V

M

G

D

D

T

N

A

T

V

D

A

L

L

I

Q

I

V

L

R

R

D

N

A

A

L

L

D

D

S

L

I

L

D

L

A

P

D

K

I

L

R

A

E

R

L

V

R

I

G

S

I

R

L

L

V

A

D

D

L

F

S

A

K

K

K

E

H

R

R

A

D

S

T

L

P

P

M

T

A

L

G

G

R

F

T
|
T

H
|
H

L

F

Q

Q

Q

P

A

A

L

Q

P

L

V

T

T

T

F

V

G

G

Y

K

K

R

T

C

A

C

I

L

W

W

L

I

A

Q

M

D

F

L

D

C

R

M

H

D

Q

L

Q

Q

R

N

I

L

A

K

Q

R

L

V

R

R

P

D

R

D

V

L

A

R

V

F

V

R

E

G

F

V

A

K

G

G

A

T

G

G

T

T

L

Q

A

A

S

S

L

F

-

L

-

Q

-

L

-

F

-

E

G

G

D

D

-

D

-

H

-

K

-

V

K

E

G

Q

F

L

D

D

V

K

Q

M

K

V

A

T

L

E

A

K

A

A

E

G

L

F

Q

K

L

R

G

A

V

F

P

I

A

I

T

T

T

G

W
x
Q

H

T

V

Y

A

T

R

R

D

K

G

V

F

D

A

I

E

E

A

V

V

L

N

S

L

V

L

L

A

A

L

S

V

L

T

G

G

A

S

S

L

V

G

H

K

K

I

I

A

C

T

T

D

D

I

I

M

R

I

L

M

L

A

A

S

N

N

-

E

-

F

L

G

K

E

E

V

M

Y

E

E

E

P

P

F

-

V

-

K

-

G

-

R

-

G

-

A

-

S

-

T

-

M

-

P

-

Q

-

K
|
K

R

R

N

N

P

P

I

M

S

R

S

S

E
|
E

L
x
R

M

C

L

C

A

S

A

L

A

A

K

-

A

-

V

-

R

R

Q

H

Q

L

A

M

G

T

L

L

M

V

V

M

D

D

-

P

-

L

-

Q

-

T

A

A

M

S

I

V

Q

Q

D

W

F

F

E

E

R

R

A

T

T
x
L

G

D

P

D

W
x
S

H
x
A

A

N

E

R

W
x
R

I

I

A

C

I

L

P

A

E

E

S

A

F

F

I

L

L

T

A

A

S

D

G

T

A

I

L

L

H

N

Q

T

A

L

K

Q

F

N

A

I

L

S

G

E

G

G

L

L

I

V

V

V

D

Y

T

P

D

K

R

V

M

I

K

E

H

R

N

R

L

I

G

R

I

Q

S

E

K

L

G

P

L

F

I

M

V

A

A

T

E

E

A

N

V

I

M

I

M

M

Q

A

M

M

A

V

P

K

Y

A

T

G

G

G

-

S

R

R

Q

Q

Q

D

A

C

H

H

D

E

I

K

V

I

Y

-

D

-

A

-

C

-

R

R

T

V

V

L

N

S

E

Q

S

Q

G

A

T

S

L

V

A

V

E

K

A

Q

L

E

A

G

L

D

P

N

E

D

V

L

T

I

R

E

H

R

F

I

D

Q

R

A

D

D

A

A

-

Y

-

F

-

S

-

P

-

I

-

H

-

S

-

Q

I

L

N

D

A

H

M

L

T

L

D

D

P

P

A

S

N

S

Y

F

L

T

G

G

L

R

A

A

P

S

Q

Q

M

Q

V

V

D

Q

R

R

active site: H79 (≠ Y88), T151 (= T160), H152 (= H161), K275 (= K290), E282 (= E297)
binding n-{[5-amino-1-(5-o-phosphono-beta-d-arabinofuranosyl)-1h-imidazol-4-yl]carbonyl}-l-aspartic acid: Y14 (≠ F22), R78 (≠ G87), H79 (≠ Y88), D80 (≠ P89), T104 (= T113), S105 (≠ T114), Q234 (≠ W234), K275 (= K290), R283 (≠ L298), L311 (≠ T325), S314 (≠ W328), A315 (≠ H329), R318 (≠ W332)

Sites not aligning to the query:

binding n-{[5-amino-1-(5-o-phosphono-beta-d-arabinofuranosyl)-1h-imidazol-4-yl]carbonyl}-l-aspartic acid: 13

5eytA Crystal structure of adenylosuccinate lyase from schistosoma mansoni in complex with amp (see paper)
26% identity, 82% coverage: 22:394/456 of query aligns to 13:389/472 of 5eytA

query
sites
5eytA

F
x
Y

G

A

T

S

A

R

K

E

M

M

R

V

A

C

L

N

F

F

S

G

D

E

Y

K

A

R

L

K

V

V

Q

I

R

L

Y

W

I

R

D

Q

V

L

E

W

V

I

A

W

L

L

A

A

K

E

A

T

E

Q

A

K

R

E

V

L

G

G

V

F

I

D

P

I

T

T

D

D

A

E

A

-

D

Q

V

I

I

N

A

E

R

M

E

K

S

S

Q

Q

I

R

E

D

R

S

I

V

D

D

F

F

D

G

H

T

M

A

R

A

E

A

E

E

T

E

D

K

I

A

V

R

G

R

Y
x
H

P

D

I

V

L

M

P

A

L

H

V

V

H

Y

Q

T

L

F

V

A

G

L

M

A

C

C

G

P

E

K

A

A

G

A

R

P

Y

I

V

I

H

H

W

L

G

G

A

A

T

T

T

S

Q

C

D

F

I

V

M

G

D

D

T

N

A

A

V

D

A

L

L

I

Q

M

V

L

R

K

D

D

A

G

L

L

D

N

S

I

I

L

D

L

A

P

D

K

I

V

R

A

E

R

L

C

R

I

G

D

I

R

L

L

V

A

D

K

L

K

S

A

K

M

K

L

H

H

R

K

D

S

T

L

P

I

M

C

A

L

G

A

R

R

T
|
T

H
|
H

L

L

Q

Q

Q

P

A

A

L

Q

P

P

V

T

T

T

F

M

G

G

Y

R

K

R

T

I

A

C

I

M

W

W

L

I

A

Q

M

D

F

L

D

L

R

L

H

D

Q

L

Q

E

R

N

I

L

A

E

Q

R

L

L

R

K

P

N

R

H

V

T

A

I

V

R

V

F

E

R

F

G

A

A

-

K

G

G

A

A

A

V

G

G

T

T

L

Q

A

A

S

S

L

F

G

M

D

D

-

L

-

F

K

Q

G

G

F

-

D

D

V

H

Q

Q

K

K

A

V

L

I

A

K

A

L

E

D

L

E

Q

I

L

L

G

T

V

K

P

K

A

S

-

G

-

F

-

Q

T

R

T

S

W

W

H

C

V

V

A

T

R

G

D

Q

G

T

F

Y

A

P

-

R

-

K

-

V

-

D

-

I

E

E

A

I

V

T

N

N

L

A

L

L

A

S

L

N

V

I

T

G

G

A

S

T

L

V

G

H

K

K

I

I

A

C

T

T

D

D

I

I

M

R

I

L

M

L

A

S

S

S

N

-

E

-

F

F

G

H

E

E

V

V

Y

E

E

E

P

P

F

F

V

-

K

-

G

-

R

-

G

-

A

-

S

E

S

T

T

K

M

M

P

P

Q

Y

K
|
K

R

R

N

N

P

P

I

I

S

R

S

S

E
|
E

L
x
R

M

A

L

C

A

S

A

L

A

A

K

R

A

Y

V

L

R

M

Q

H

Q

I

A

S

G

T

L

S

M

M

V

V

D

S

A

T

M

V

-

S

I

V

Q

Q

D

W

F

L

E

E

R

R

A

S

T

L

G

D

P

D

W

S

H

A

A

I

E

R

W

R

I

I

A

V

I

L

P

P

E

E

S

A

F

F

I

L

L

A

A

A

S

D

G

A

A

C

L

L

H

T

Q

L

A

L

K

Q

F

N

A

I

L

A

G

E

G

G

L

L

I

I

V

V

D

Y

T

P

D

M

R

V

M

M

K

E

H

A

N

N

L

L

G

N

I

S

S

E

K

L

G

P

L

F

I

L

V

V

A

V

E

E

A

R

V

I

M

L

M

V

Q

K

M

M

A

V

P

S

Y

E

-

G

-

A

T

A

G

N

R

R

Q

Q

Q

E

A

C

H

H

D

E

active site: H78 (≠ Y88), T150 (= T160), H151 (= H161), K282 (= K290), E289 (= E297)
binding adenosine monophosphate: Y13 (≠ F22), R290 (≠ L298)

Sites not aligning to the query:

binding adenosine monophosphate: 12

5nxaC Crystal structure of neanderthal adenylosuccinate lyase (adsl)in complex with its products aicar and fumarate (see paper)
25% identity, 80% coverage: 22:387/456 of query aligns to 13:374/418 of 5nxaC

query
sites
5nxaC

F
x
Y

G

A

T

S

A

P

K

E

M

M

R

C

A

F

L

V

F

F

S

S

D

D

Y

R

A

Y

L

K

V

F

Q

R

R

T

Y

W

I

R

D

Q

V

L

E

W

V

L

A

W

L

L

A

A

K

E

A

A

E

E

A

Q

R

T

V

L

G

G

V

L

I

P

P

I

T

T

D

D

A

E

A

-

D

Q

V

I

I

Q

A

E

R

M

E

K

S

S

Q

N

I

L

E

E

R

N

I

I

D

D

F

F

D

K

H

M

M

A

R

A

E

E

T

E

D

K

I

R

V

L

G
x
R

Y
x
H

P
x
D

I

V

L

M

P

A

L

H

V

V

H

H

Q

T

L

F

V

G

G

H

M

C

C

C

G

P

E

K

A

A

G

A

R

G

Y

I

V

I

H

H

W

L

G

G

A

A

T
|
T

T
x
S

Q

C

D

Y

I

V

M

G

D

D

T

N

A

T

V

D

A

L

L

I

Q

I

V

L

R

R

D

N

A

A

L

L

D

D

S

L

I

L

D

L

A

P

D

K

I

L

R

A

E

R

L

V

R

I

G

S

I

R

L

L

V

A

D

D

L

F

S

A

K

K

K

E

H

R

R

A

D

S

T

L

P

P

M

T

A

L

G

G

R

F

T
|
T

H
|
H

L

F

Q

Q

Q

P

A

A

L

Q

P

L

V

T

T

T

F

V

G

G

Y

K

K

R

T

C

A

C

I

L

W

W

L

I

A

Q

M

D

F

L

D

C

R

M

H

D

Q

L

Q

Q

R

N

I

L

A

K

Q

R

L

V

R

R

P

D

R

D

V

L

A

R

V

F

V

R

E

G

F

V

A

K

G

G

A

T

G

G

T

T

L

Q

A

A

S

S

L

F

-

L

-

Q

-

L

-

F

-

E

G

G

D

D

-

D

-

H

-

K

-

V

K

E

G

Q

F

L

D

D

V

K

Q

M

K

V

A

T

L

E

A

K

A

A

E

G

L

F

Q

K

L

R

G

A

V

F

P

I

A

I

T

T

T

G

W
x
Q

H

T

V

Y

A

T

R

R

D

K

G

V

F

D

A

I

E

E

A

V

V

L

N

S

L

V

L

L

A

A

L

S

V

L

T

G

G

A

S

S

L

V

G

H

K

K

I

I

A

C

T

T

D

D

I

I

M

R

I

L

M

L

A

A

S

N

N

-

E

-

F

L

G

K

E

E

V

M

Y

E

E

E

P

P

F

F

V

-

K

-

G

-

R

-

G

-

A

-

S

-

T

-

M

-

P

-

Q

E

K
|
K

R

R

N

N

P

P

I

M

S

R

S

S

E
|
E

L
x
R

M

C

L

C

A

S

A

L

A

A

K

-

A

-

V

-

R

R

Q

H

Q

L

A

M

G

T

L

L

M

V

V

M

D

D

-

P

-

L

-

Q

-

T

A

A

M

S

I

V

Q

Q

D

W

F

F

E

E

R

R

A

T

T
x
L

G

D

P

D

W
x
S

H
x
A

A

N

E

R

W
x
R

I

I

A

C

I

L

P

A

E

E

S

A

F

F

I

L

L

T

A

A

S

D

G

T

A

I

L

L

H

N

Q

T

A

L

K

Q

F

N

A

I

L

S

G

E

G

G

L

L

I

V

V

V

D

Y

T

P

D

K

R

V

M

I

K

E

H

R

N

R

L

I

G

R

I

Q

S

E

K

L

G

P

L

F

I

M

V

A

A

T

E

E

A

N

V

I

M

I

M

M

Q

A

M

M

A

V

P

K

Y

H

T

S

active site: H78 (≠ Y88), T150 (= T160), H151 (= H161), K276 (= K290), E283 (= E297)
binding aminoimidazole 4-carboxamide ribonucleotide: R77 (≠ G87), H78 (≠ Y88), D79 (≠ P89), Q233 (≠ W234), L312 (≠ T325), S315 (≠ W328), A316 (≠ H329), R319 (≠ W332)
binding fumaric acid: H78 (≠ Y88), T103 (= T113), S104 (≠ T114), Q233 (≠ W234)
binding n-{[5-amino-1-(5-o-phosphono-beta-d-arabinofuranosyl)-1h-imidazol-4-yl]carbonyl}-l-aspartic acid: Y13 (≠ F22), T150 (= T160), H151 (= H161), K276 (= K290), R284 (≠ L298)

Sites not aligning to the query:

binding n-{[5-amino-1-(5-o-phosphono-beta-d-arabinofuranosyl)-1h-imidazol-4-yl]carbonyl}-l-aspartic acid: 12

5nx9C Crystal structure of neanderthal adenylosuccinate lyase (adsl) in complex with its products amp and fumarate (see paper)
23% identity, 94% coverage: 22:448/456 of query aligns to 13:428/441 of 5nx9C

query
sites
5nx9C

F
x
Y

G

A

T

S

A

P

K

E

M

M

R

C

A

F

L

V

F

F

S

S

D

D

Y

R

A

Y

L

K

V

F

Q

R

R

T

Y

W

I

R

D

Q

V

L

E

W

V

L

A

W

L

L

A

A

K

E

A

A

E

E

A

Q

R

T

V

L

G

G

V

L

I

P

P

I

T

T

D

D

A

E

A

-

D

Q

V

I

I

Q

A

E

R

M

E

K

S

S

Q

N

I

L

E

E

R

N

I

I

D

D

F

F

D

K

H

M

M

A

R

A

E

E

T

E

D

K

I

R

V

L

G
x
R

Y
x
H

P
x
D

I

V

L

M

P

A

L

H

V

V

H

H

Q

T

L

F

V

G

G

H

M

C

C

C

G

P

E

K

A

A

G

A

R

G

Y

I

V

I

H

H

W

L

G

G

A

A

T
|
T

T
x
S

Q

C

D

Y

I

V

M

G

D

D

T

N

A

T

V

D

A

L

L

I

Q

I

V

L

R

R

D

N

A

A

L

L

D

D

S

L

I

L

D

L

A

P

D

K

I

L

R

A

E

R

L

V

R

I

G

S

I

R

L

L

V

A

D

D

L

F

S

A

K

K

K

E

H

R

R

A

D

S

T

L

P

P

M

T

A

L

G

G

R

F

T
|
T

H
|
H

L

F

Q

Q

Q

P

A

A

L

Q

P

L

V

T

T

T

F

V

G

G

Y

K

K

R

T

C

A

C

I

L

W

W

L

I

A

Q

M

D

F

L

D

C

R

M

H

D

Q

L

Q

Q

R

N

I

L

A

K

Q

R

L

V

R

R

P

D

R

D

V

L

A

R

V

F

V

R

E

G

F

V

A

K

G

G

A

T

G

G

T

T

L

Q

A

A

S

S

L

F

-

L

-

Q

-

L

-

F

-

E

G

G

D

D

-

D

-

H

-

K

-

V

K

E

G

Q

F

L

D

D

V

K

Q

M

K

V

A

T

L

E

A

K

A

A

E

G

L

F

Q

K

L

R

G

A

V

F

P

I

A

I

T

T

T

G

W
x
Q

H

T

V

Y

A

T

R

R

D

K

G

V

F

D

A

I

E

E

A

V

V

L

N

S

L

V

L

L

A

A

L

S

V

L

T

G

G

A

S

S

L

V

G

H

K

K

I

I

A

C

T

T

D

D

I

I

M

R

I

L

M

L

A

A

S

N

N

-

E

-

F

L

G

K

E

E

V

M

Y

E

E

E

P

P

F

-

V

-

K

-

G

-

R

-

G

-

A

-

S

-

T

-

M

-

P

-

Q

-

K

-

R

R

N

N

P

P

I
x
M

S

R

S

S

E
|
E

L
x
R

M

C

L

C

A

S

A

L

A

A

K

-

A

-

V

-

R

R

Q

H

Q

L

A

M

G

T

L

L

M

V

V

M

D

D

-

P

-

L

-

Q

-

T

A

A

M

S

I

V

Q

Q

D

W

F

F

E

E

R

R

A

T

T
x
L

G

D

P

D

W
x
S

H
x
A

A

N

E

R

W
x
R

I

I

A

C

I

L

P

A

E

E

S

A

F

F

I

L

L

T

A

A

S

D

G

T

A

I

L

L

H

N

Q

T

A

L

K

Q

F

N

A

I

L

S

G

E

G

G

L

L

I

V

V

V

D

Y

T

P

D

K

R

V

M

I

K

E

H

R

N

R

L

I

G

R

I

Q

S

E

K

L

G

P

L

F

I

M

V

A

A

T

E

E

A

N

V

I

M

I

M

M

Q

A

M

M

A

-

P

-

Y

-

T

-

G

-

R

-

Q

-

A

-

H

-

D

-

I

-

V

V

Y

H

D

E

A

K

C

I

R

R

T

V

V

L

N

S

E

Q

S

Q

G

A

T

S

L

V

A

V

E

K

A

Q

L

E

A

G

L

D

P

N

E

D

V

L

T

I

R

E

H

R

F

I

D

Q

R

A

D

D

A

A

-

Y

-

F

-

S

-

P

-

I

-

H

-

S

-

Q

I

L

N

D

A

H

M

L

T

L

D

D

P

P

A

S

N

S

Y

F

L

T

G

G

L

R

A

A

P

S

Q

Q

M

Q

V

V

D

Q

R

R

active site: H78 (≠ Y88), T150 (= T160), H151 (= H161), E280 (= E297)
binding 2-[9-(3,4-dihydroxy-5-phosphonooxymethyl-tetrahydro-furan-2-yl)-9h-purin-6-ylamino]-succinic acid: Y13 (≠ F22), R77 (≠ G87), H78 (≠ Y88), D79 (≠ P89), T103 (= T113), S104 (≠ T114), Q233 (≠ W234), M277 (≠ I294), R281 (≠ L298), L309 (≠ T325), S312 (≠ W328), A313 (≠ H329), R316 (≠ W332)
binding fumaric acid: T150 (= T160), H151 (= H161)

Sites not aligning to the query:

binding 2-[9-(3,4-dihydroxy-5-phosphonooxymethyl-tetrahydro-furan-2-yl)-9h-purin-6-ylamino]-succinic acid: 12

5nxaB Crystal structure of neanderthal adenylosuccinate lyase (adsl)in complex with its products aicar and fumarate (see paper)
25% identity, 75% coverage: 107:448/456 of query aligns to 36:390/415 of 5nxaB

query
sites
5nxaB

Y

W

V

L

H

W

W

L

G

G

A

A

T

T

T
x
S

Q

C

D

Y

I

V

M

G

D

D

T

N

A

T

V

D

A

L

L

I

Q

I

V

L

R

R

D

N

A

A

L

L

D

D

S

L

I

L

D

L

A

P

D

K

I

L

R

A

E

R

L

V

R

I

G

S

I

R

L

L

V

A

D

D

L

F

S

A

K

K

K

E

H

R

R

A

D

S

T

L

P

P

M

T

A

L

G

G

R

F

T
|
T

H
|
H

L

F

Q

Q

Q

P

A

A

L

Q

P

L

V

T

T

T

F

V

G

G

Y

K

K

R

T

C

A

C

I

L

W

W

L

I

A

Q

M

D

F

L

D

C

R

M

H

D

Q

L

Q

Q

R

N

I

L

A

K

Q

R

L

V

R

R

P

D

R

D

V

L

A

R

V

F

V

R

E

G

F

V

A

K

G

G

A

T

G

G

T

T

L

Q

A

A

S

S

L

F

-

L

-

Q

-

L

-

F

-

E

G

G

D

D

-

D

-

H

-

K

-

V

K

E

G

Q

F

L

D

D

V

K

Q

M

K

V

A

T

L

E

A

K

A

A

E

G

L

F

Q

K

L

R

G

A

V

F

P

I

A

I

T

T

T

G

W
x
Q

H

T

V

Y

A

T

R

R

D

K

G

V

F

D

A

I

E

E

A

V

V

L

N

S

L

V

L

L

A

A

L

S

V

L

T

G

G

A

S

S

L

V

G

H

K

K

I

I

A

C

T

T

D

D

I

I

M

R

I

L

M

L

A

A

S

N

N

-

E

-

F

L

G

K

E

E

V

M

Y

E

E

E

P

P

F

F

V

E

K

K

G

Q

R

Q

G

I

A

G

S
|
S

T

A

M
|
M

P

P

Q

Y

K
|
K

R

R

N
|
N

P

P

I
x
M

S

R

S

S

E
|
E

L
x
R

M

C

L

C

A

S

A

L

A

A

K

-

A

-

V

-

R

R

Q

H

Q

L

A

M

G

T

L

L

M

V

V

M

D

D

-

P

-

L

-

Q

-

T

A

A

M

S

I

V

Q

Q

D

W

F

F

E

E

R

R

A

T

T
x
L

G

D

P

D

W
x
S

H
x
A

A

N

E

R

W
x
R

I

I

A

C

I

L

P

A

E

E

S

A

F

F

I

L

L

T

A

A

S

D

G

T

A

I

L

L

H

N

Q

T

A

L

K

Q

F

N

A

I

L

S

G

E

G

G

L

L

I

V

V

V

D

Y

T

P

D

K

R

V

M

I

K

E

H

R

N

R

L

I

G

R

I

Q

S

E

K

L

G

P

L

F

I

M

V

A

A

T

E

E

A

N

V

I

M

I

M

M

Q

A

M

M

A

V

P

K

Y

A

T

G

G

G

-

S

R

R

Q

Q

Q

D

A

C

H

H

D

E

I

K

V

I

Y

-

D

-

A

-

C

-

R

R

T

V

V

L

N

S

E

Q

S

Q

G

A

T

S

L

V

A

V

E

K

A

Q

L

E

A

G

L

D

P

N

E

D

V

L

T

I

R

E

H

R

F

I

D

Q

R

A

D

D

A

A

-

Y

-

F

-

S

-

P

-

I

-

H

-

S

-

Q

I

L

N

D

A

H

M

L

T

L

D

D

P

P

A

S

N

S

Y

F

L

T

G

G

L

R

A

A

P

S

Q

Q

M

Q

V

V

D

Q

R

R

active site: T89 (= T160), H90 (= H161), S221 (= S285), K226 (= K290), E233 (= E297)
binding aminoimidazole 4-carboxamide ribonucleotide: M230 (≠ I294), R234 (≠ L298)
binding fumaric acid: S220 (= S284), S221 (= S285), M223 (= M287), K226 (= K290), N228 (= N292)
binding n-{[5-amino-1-(5-o-phosphono-beta-d-arabinofuranosyl)-1h-imidazol-4-yl]carbonyl}-l-aspartic acid: S43 (≠ T114), T89 (= T160), H90 (= H161), Q172 (≠ W234), L262 (≠ T325), S265 (≠ W328), A266 (≠ H329), R269 (≠ W332)

Sites not aligning to the query:

binding aminoimidazole 4-carboxamide ribonucleotide: 13, 14

1tj7B Structure determination and refinement at 2.44 a resolution of argininosuccinate lyase from e. Coli (see paper)
25% identity, 93% coverage: 29:453/456 of query aligns to 16:446/451 of 1tj7B

query
sites
1tj7B

A

S

L

L

F

R

S

F

D

D

Y

Y

A

R

L

L

V

A

Q

E

R

Q

Y

D

I

I

D

V

V

G

E

S

V

V

A

A

L

W

A

S

K

K

A

A

E

L

A

V

R

T

V

V

G

G

V

V

I

L

P

T

T

A

D

E

A

E

-

Q

-

A

-

Q

-

L

-

E

-

A

-

L

-

N

-

V

-

L

A

E

D

D

V

V

I

R

A

A

R

R

E

P

S

Q

Q

Q

I

I

E

L

R

E

I

S

D

D

F

A

D

E

H

-

M

-

R

-

E

-

T

-

D

D

I

I

V

H

G

S

Y

W

P

-

I

-

L

-

P

-

L

V

V

E

H

G

Q

K

L

L

V

I

G

D

M

K

C

V

G

G

E

Q

A

L

G

G

R

K

Y

K

V

L

H

H

W

T

G

G

A

R

T

S

T

R

Q

N

D

D

I

Q

M

V

D

A

T

T

A

D

V

L

A

K

L

L

Q

W

V

C

R

K

D

D

A

T

L

V

D

S

S

E

I

L

D

L

A

T

D

A

I

N

R

R

E

Q

L

L

R

Q

G

S

I

A

L

L

V

V

D

E

L

T

S

A

K

Q

K

N

H

N

R

Q

D

D

T

A

P

V

M

M

A

P

G

G

R

Y

T

T

H
|
H

L

L

Q

Q

Q

R

A

A

L

Q

P

P

V

V

T

T

F

F

G

A

Y

H

K

W

T

C

A

L

I

A

W

Y

L

V

A

E

M

M

F

L

D

A

R

R

H

D

Q

E

Q

S

R

R

I

L

A

Q

Q

D

L

A

R

L

P

K

R

R

V

L

A

D

V

V

V

S

E

P

F

L

A

G

G

C

A

G

A

A

G

-

T

-

L

-

A

-

S

-

L

L

G

A

D

G

K

T

G

A

F

Y

D

E

V

I

Q

D

K

R

A

E

L

Q

A

L

A

A

E

G

L

W

Q

-

L

L

G

G

V

F

P

A

A

S

T

A

T

T

W

R

H

N

V

S

A

L

-

D

-

S

-

V

-

S

-

D

R

R

D

D

G

H

F

V

A

L

E

E

A

L

V

L

N

S

L

A

A

A

L

I

V

G

T

M

G

V

S

H

L

L

G

S

K

R

I

F

A

A

T

E

D

D

I

L

M

I

I

F

M

F

A

N

S

T

N

G

E

E

-

A

-

G

F

F

G

V

E

E

V

L

Y

S

E

D

P

R

F

V

V

T

K

S

G

G

R

-

G

-

A

-

S
|
S

S

S

T

L

M
|
M

P

P

Q

Q

K
|
K

R

K

N

N

P

P

I

D

S

A

S

L

E
|
E

L

L

M

I

L

-

A

-

A

R

A

G

K

K

A

C

V

G

R

R

Q

V

Q

Q

A

G

G

A

L

L

M

T

V

-

D

-

A

G

M

M

I

M

Q

M

D

T

F

L

E

K

R

G

A

L

T

P

G

L

P

A

W

Y

H

N

A

K

E

D

W

M

I

Q

A

E

I

D

P

K

E

E

S

G

F

L

I

F

L

D

A

A

-

L

-

D

-

T

-

W

S

L

G

D

A

C

L

L

H

H

Q

M

A

A

K

A

F

L

A

V

L

L

G

D

G

G

L

I

I

Q

V

V

D

K

T

R

D

P

R

R

M

C

K

Q

H

E

-

A

N

Q

L

Q

G

G

I

Y

S

A

K

N

G

A

L

T

I

E

V

L

A

A

E

D

A

Y

V

L

M

V

M

A

Q

K

M

G

A

V

P

P

Y

F

T

-

G

-

R

-

Q

R

Q

E

A

A

H

H

D

H

I

I

V

V

Y

G

D

E

A

A

C

V

R

V

T

E

V

A

N

I

E

R

S

Q

G

G

G

K

T

P

L

L

A

E

E

D

-

L

A

P

L

L

A

S

A

E

L

L

P

Q

E

K

V

F

T

S

R

Q

H

V

F

I

D

D

R

E

D

D

A

V

I

Y

-

P

-

I

-

L

-

S

-

L

-

Q

N

S

A

C

M

L

T

D

D

K

P

R

A

A

N

A

Y

K

L

G

G

G

L

V

A

S

P

P

Q

Q

M

Q

V

V

D

A

R

Q

A

A

I

I

A

A

L

F

S

A

active site: H150 (= H161), S271 (= S284), K277 (= K290), E284 (= E297)
binding phosphate ion: S271 (= S284), M274 (= M287), K277 (= K290)

3gzhA Crystal structure of phosphate-bound adenylosuccinate lyase from e. Coli (see paper)
25% identity, 92% coverage: 27:447/456 of query aligns to 36:466/469 of 3gzhA

query
sites
3gzhA

M

L

R

R

A

G

L

I

F

F

S

S

D

E

Y

Y

A

G

L

L

V

L

Q

K

R

F

Y

R

I

V

D

Q

V

V

E

E

V

V

A

R

L

W

A

L

K

Q

-

K

-

L

-

A

A

A

E

H

A

A

R

A

V

I

G

K

V

E

I

V

P

P

T

A

D

F

A

A

D

D

V

A

I

I

-

G

-

Y

-

L

-

D

-

A

-

I

-

V

-

A

-

N

-

F

-

S

-

E

-

D

-

A

A

A

R

R

E

I

S

K

Q

T

I

I

E

E

R

R

I

T

D

-

F

-

D

-

H

-

M

T

R

N

E
x
H

E

D

T

V

D

K

I

A

V

V

G

E

Y

Y

P

F

I

L

L

K

P

E

L

K

V

V

H

A

Q

E

L

I

V

P

G

E

M

L

C

H

G

A

E

-

A

V

G

S

R

E

Y

F

V

I

H

H

W

F

G

A

A

C

T
|
T

T
x
S

Q

E

D

D

I

I

M

N

D

N

T

L

A

S

V

H

A

A

L

L

Q

M

V

L

R

K

D

T

A

A

L

R

D

D

S

E

I

V

D

I

A

L

D

P

I

Y

-

W

R

R

E

Q

L

L

R

I

G

D

I

G

L

I

V

K

D

D

L

L

S

A

K

V

K

Q

H

Y

R

R

D

D

T

I

P

P

M

L

A

L

G

S

R

R

T
|
T

H
|
H

L

G

Q

Q

Q

P

A

A

L

T

P

P

V

S

T

T

F

I

G

G

Y

K

K

E

T

M

A

A

I

N

W

V

L

A

A

Y

M

R

F

M

D

E

R

R

H

Q

Q

Y

Q

R

R

Q

I

L

A

N

Q

Q

L

V

-

E

-

I

-

L

-

G

R

K

P

I

R

N

V

G

A

A

V

V

V

G

E

N

F

Y

A

N

G

A

A

H

A

I

G

A

T

A

L

Y

A

P

S

E

L

V

G

D

D

W

K

H

G

Q

F

F

D

S

V

E

Q

E

K

F

A

V

L

T

A

S

A

L

E

G

L

I

Q

Q

L

W

G

N

V

-

P

P

A

Y

T

T

W

Q

H

I

V

E

A

P

R

H

D

D

G

Y

F

I

A

A

E

E

A

L

V

F

N

D

L

C

L

V

A

A

L

R

V

F

T

N

G

T

S

I

L

L

G

I

K

D

I

F

A

D

T

R

D

D

I

V

M

W

-

G

I

Y

M

I

A

A

S

L

N

N

E

H

F

F

G

K

E

-

V

-

Y

-

E

Q

P

K

F

T

V

I

K

A

G

G

R

E

G

I

A

G

S

S

S
|
S

T

T

M

M

P

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active site: H104 (≠ E81), T183 (= T160), H184 (= H161), S309 (= S285), K314 (= K290), E321 (vs. gap)
binding phosphate ion: H104 (≠ E81), T135 (= T113), S136 (≠ T114), S353 (≠ A324), T354 (= T325), R357 (≠ W328)

P0AB89 Adenylosuccinate lyase; ASL; Adenylosuccinase; ASase; EC 4.3.2.2 from Escherichia coli (strain K12) (see 2 papers)
25% identity, 92% coverage: 27:447/456 of query aligns to 23:453/456 of P0AB89

query
sites
P0AB89

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NHD 90:92 (≠ REE 80:82) binding ; binding
H91 (≠ E81) binding
K94 (≠ D84) modified: N6-acetyllysine
TS 122:123 (≠ TT 113:114) binding ; binding
H171 (= H161) mutation H->A,N: Reduces catalytic activity about 500-fold.
Q247 (≠ W234) binding ; binding ; binding
S295 (= S284) mutation to A: Reduces catalytic activity about 1000-fold.
S296 (= S285) binding ; binding
KVN 301:303 (≠ KRN 290:292) binding ; binding
N309 (≠ S295) binding ; binding
R335 (≠ Q319) binding ; binding
STVLR 340:344 (≠ ATGPW 324:328) binding ; binding
K366 (≠ G355) modified: N6-acetyllysine

Sites not aligning to the query:

15:16 binding ; binding

P04424 Argininosuccinate lyase; ASAL; Arginosuccinase; EC 4.3.2.1 from Homo sapiens (Human) (see 12 papers)
27% identity, 48% coverage: 96:314/456 of query aligns to 95:312/464 of P04424

query
sites
P04424

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R95 (≠ Q96) to C: in ARGINSA; loss of argininosuccinate lyase activity; dbSNP:rs28940585
R113 (≠ T114) to Q: in ARGINSA; complete loss of argininosuccinate lyase activity; no effect on protein expression; no effect on nitric oxide production; dbSNP:rs752783461
D120 (≠ A121) to E: in ARGINSA; severe
V178 (≠ A179) to M: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs28941473
T181 (≠ D182) to S: in a breast cancer sample; somatic mutation
R182 (= R183) to Q: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression; dbSNP:rs751590073
R186 (= R187) to Q: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression; dbSNP:rs752397242
G200 (≠ A201) to V: in a breast cancer sample; somatic mutation
R236 (= R238) to W: in ARGINSA; complete loss of argininosuccinate lyase activity; no effect on protein expression; no effect on NOS complex formation; dbSNP:rs761268464
D237 (= D239) to N: in ARGINSA; severe; dbSNP:rs552951774
Q286 (= Q289) to R: in ARGINSA; complete loss of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs28941472
K288 (≠ R291) modified: N6-acetyllysine; mutation to R: Refractory to inhibition by TSA and NAM and by addition of extra amino acids. No effect on protein structure.
R297 (≠ L300) to Q: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs750431938
R306 (≠ Q308) to W: in ARGINSA; severe; dbSNP:rs868834862

Sites not aligning to the query:

12 R → Q: in ARGINSA; 18-fold reduction in catalytic efficiency toward argininosuccinate; dbSNP:rs145138923
31 D → N: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs754995756
51 K→N: 2-fold reduction in activity.
69 modified: N6-acetyllysine
73 E → K: in ARGINSA; complete loss of argininosuccinate lyase activity; abolishes protein expression
87 D → G: in ARGINSA; loss of argininosuccinate lyase activity; dbSNP:rs752100894
89 H→Q: 10-fold reduction in activity.
94 R → C: in ARGINSA; severe; dbSNP:rs374304304
326 Q → L: in ARGINSA; severe
335 V → L: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression
360 M → T: in ARGINSA; loss of argininosuccinate lyase activity; may cause protein misfolding; dbSNP:rs875989948
382 M → R: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression
385 R → L: in ARGINSA; severe
388 H → Q: in ARGINSA; severe
398 A → D: in ARGINSA; impairs tetramer formation likely due to protein misfolding; loss of argininosuccinate lyase activity
456 R → W: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression; dbSNP:rs759396688

Query Sequence

>H281DRAFT_02594 FitnessBrowser__Burk376:H281DRAFT_02594
MTTRTTPLSSTVFDSILFRDAFGTAKMRALFSDYALVQRYIDVEVALAKAEARVGVIPTD
AADVIARESQIERIDFDHMREETDIVGYPILPLVHQLVGMCGEAGRYVHWGATTQDIMDT
AVALQVRDALDSIDADIRELRGILVDLSKKHRDTPMAGRTHLQQALPVTFGYKTAIWLAM
FDRHQQRIAQLRPRVAVVEFAGAAGTLASLGDKGFDVQKALAAELQLGVPATTWHVARDG
FAEAVNLLALVTGSLGKIATDIMIMASNEFGEVYEPFVKGRGASSTMPQKRNPISSELML
AAAKAVRQQAGLMVDAMIQDFERATGPWHAEWIAIPESFILASGALHQAKFALGGLIVDT
DRMKHNLGISKGLIVAEAVMMQMAPYTGRQQAHDIVYDACRTVNESGGTLAEALAALPEV
TRHFDRDAINAMTDPANYLGLAPQMVDRAIALSSEI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory