SitesBLAST
Comparing H281DRAFT_02675 FitnessBrowser__Burk376:H281DRAFT_02675 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1ffuD Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava which lacks the mo-pyranopterin moiety of the molybdenum cofactor (see paper)
52% identity, 90% coverage: 4:158/173 of query aligns to 3:156/156 of 1ffuD
- binding fe2/s2 (inorganic) cluster: C41 (= C42), S44 (≠ G45), H45 (≠ V46), C46 (= C47), G47 (= G48), C49 (= C50), C61 (= C62), C100 (= C102), G101 (= G103), C103 (= C105), C135 (= C137), C137 (= C139)
1ffvA Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava (see paper)
52% identity, 90% coverage: 4:158/173 of query aligns to 2:155/155 of 1ffvA
- binding fe2/s2 (inorganic) cluster: I38 (≠ Y40), C40 (= C42), S43 (≠ G45), C45 (= C47), G46 (= G48), C48 (= C50), C60 (= C62), C99 (= C102), G100 (= G103), C102 (= C105), C134 (= C137), C136 (= C139)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q98 (= Q101), C136 (= C139)
4zohC Crystal structure of glyceraldehyde oxidoreductase (see paper)
54% identity, 85% coverage: 10:156/173 of query aligns to 16:160/161 of 4zohC
- binding fe2/s2 (inorganic) cluster: C47 (= C42), S50 (≠ G45), C52 (= C47), G53 (= G48), C55 (= C50), C67 (= C62), C106 (= C102), G107 (= G103), C109 (= C105), C141 (= C137), C143 (= C139)
- binding pterin cytosine dinucleotide: Q105 (= Q101), C143 (= C139)
7dqxC Crystal structure of xanthine dehydrogenase family protein
53% identity, 88% coverage: 1:152/173 of query aligns to 1:152/160 of 7dqxC
- binding fe2/s2 (inorganic) cluster: C42 (= C42), G45 (= G45), V46 (= V46), C47 (= C47), C50 (= C50), R60 (= R60), C62 (= C62), Q100 (= Q101), C101 (= C102), C104 (= C105), C137 (= C137), C139 (= C139)
- binding pterin cytosine dinucleotide: Q100 (= Q101), C139 (= C139)
P19921 Carbon monoxide dehydrogenase small chain; CO dehydrogenase subunit S; CO-DH S; EC 1.2.5.3 from Afipia carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5) (Oligotropha carboxidovorans) (see 2 papers)
50% identity, 91% coverage: 1:157/173 of query aligns to 1:157/166 of P19921
- C42 (= C42) binding
- C47 (= C47) binding
- C50 (= C50) binding
- C62 (= C62) binding
- C102 (= C102) binding
- C105 (= C105) binding
- C137 (= C137) binding
- C139 (= C139) binding
1n5wD Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
51% identity, 88% coverage: 6:157/173 of query aligns to 4:155/158 of 1n5wD
- binding flavin-adenine dinucleotide: S43 (≠ G45), H44 (≠ V46)
- binding fe2/s2 (inorganic) cluster: C40 (= C42), S43 (≠ G45), C45 (= C47), G46 (= G48), C48 (= C50), C60 (= C62), C100 (= C102), G101 (= G103), C103 (= C105), C135 (= C137), C137 (= C139)
- binding pterin cytosine dinucleotide: Q99 (= Q101), C137 (= C139)
1n5wA Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
51% identity, 88% coverage: 6:157/173 of query aligns to 4:155/161 of 1n5wA
- binding flavin-adenine dinucleotide: S43 (≠ G45), H44 (≠ V46)
- binding fe2/s2 (inorganic) cluster: I38 (≠ Y40), G39 (= G41), C40 (= C42), S43 (≠ G45), C45 (= C47), G46 (= G48), C48 (= C50), C60 (= C62), C100 (= C102), G101 (= G103), C103 (= C105), C135 (= C137), C137 (= C139)
1t3qA Crystal structure of quinoline 2-oxidoreductase from pseudomonas putida 86 (see paper)
52% identity, 83% coverage: 6:148/173 of query aligns to 6:147/162 of 1t3qA
- binding fe2/s2 (inorganic) cluster: I40 (≠ Y40), C42 (= C42), E43 (= E43), G45 (= G45), C47 (= C47), G48 (= G48), C50 (= C50), R60 (= R60), C62 (= C62), C101 (= C102), G102 (= G103), C104 (= C105), C136 (= C137), C138 (= C139)
- binding pterin cytosine dinucleotide: Q100 (= Q101), C138 (= C139)
5y6qA Crystal structure of an aldehyde oxidase from methylobacillus sp. Ky4400 (see paper)
47% identity, 88% coverage: 2:153/173 of query aligns to 1:152/157 of 5y6qA
- binding fe2/s2 (inorganic) cluster: G40 (= G41), C41 (= C42), D42 (≠ E43), G44 (= G45), C46 (= C47), G47 (= G48), C49 (= C50), C61 (= C62), C101 (= C102), G102 (= G103), C104 (= C105), C136 (= C137), C138 (= C139)
- binding pterin cytosine dinucleotide: Q100 (= Q101), C138 (= C139)
1sb3C Structure of 4-hydroxybenzoyl-coa reductase from thauera aromatica (see paper)
51% identity, 86% coverage: 6:153/173 of query aligns to 5:151/161 of 1sb3C
- binding fe2/s2 (inorganic) cluster: Q39 (≠ Y40), C41 (= C42), G44 (= G45), C46 (= C47), G47 (= G48), C49 (= C50), C61 (= C62), C100 (= C102), G101 (= G103), C103 (= C105), C135 (= C137), C137 (= C139)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q101), C137 (= C139)
3hrdD Crystal structure of nicotinate dehydrogenase (see paper)
43% identity, 90% coverage: 1:155/173 of query aligns to 1:154/160 of 3hrdD
- binding flavin-adenine dinucleotide: E44 (≠ H44), G45 (= G45), E46 (≠ V46)
- binding fe2/s2 (inorganic) cluster: E40 (≠ Y40), C42 (= C42), S43 (≠ E43), G45 (= G45), C47 (= C47), G48 (= G48), C50 (= C50), C62 (= C62), Q100 (= Q101), C101 (= C102), G102 (= G103), C104 (= C105), C136 (= C137), C138 (= C139)
- binding pterin cytosine dinucleotide: Q100 (= Q101), C138 (= C139)
Q0QLF3 Nicotinate dehydrogenase small FeS subunit; NDH; Nicotinic acid hydroxylase small FeS subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see paper)
43% identity, 90% coverage: 1:155/173 of query aligns to 1:154/157 of Q0QLF3
- C42 (= C42) binding
- C47 (= C47) binding
- C50 (= C50) binding
- C62 (= C62) binding
- C101 (= C102) binding
- C104 (= C105) binding
- C136 (= C137) binding
- C138 (= C139) binding
1dgjA Crystal structure of the aldehyde oxidoreductase from desulfovibrio desulfuricans atcc 27774 (see paper)
42% identity, 92% coverage: 1:160/173 of query aligns to 1:160/906 of 1dgjA
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y40), G39 (= G41), C40 (= C42), G41 (≠ E43), G43 (= G45), Q44 (≠ V46), C45 (= C47), G46 (= G48), C48 (= C50), R58 (= R60), C60 (= C62), C100 (= C102), G101 (= G103), C103 (= C105), C137 (= C137), C139 (= C139)
- binding pterin cytosine dinucleotide: Q99 (= Q101), C139 (= C139)
Sites not aligning to the query:
- active site: 391, 427, 503, 507, 535, 869, 870
- binding molybdenum (iv)oxide: 424, 535, 698, 869
- binding pterin cytosine dinucleotide: 423, 424, 535, 652, 655, 656, 657, 658, 697, 698, 700, 702, 703, 799, 800, 803, 804, 807, 865, 866, 867, 868, 869
P77165 Aldehyde oxidoreductase iron-sulfur-binding subunit PaoA; EC 1.2.99.6 from Escherichia coli (strain K12) (see paper)
42% identity, 90% coverage: 2:157/173 of query aligns to 59:228/229 of P77165
- C99 (= C42) binding
- C104 (= C47) binding
- G105 (= G48) binding
- C107 (= C50) binding
- C119 (= C62) binding
- C158 (= C102) binding
- C161 (= C105) binding
- C208 (= C137) binding
- C210 (= C139) binding
4usaA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with trans-cinnamaldehyde (see paper)
40% identity, 88% coverage: 8:160/173 of query aligns to 6:160/907 of 4usaA
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y40), C40 (= C42), E41 (= E43), G43 (= G45), C45 (= C47), G46 (= G48), C48 (= C50), R58 (= R60), C60 (= C62), C100 (= C102), G101 (= G103), C103 (= C105), C137 (= C137), C139 (= C139)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q101), C139 (= C139)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 531, 532, 535, 539
- binding hydrocinnamic acid: 255, 425, 494, 497, 535, 626
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4us9A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with 3- phenylpropionaldehyde (see paper)
40% identity, 88% coverage: 8:160/173 of query aligns to 6:160/907 of 4us9A
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y40), C40 (= C42), E41 (= E43), G43 (= G45), C45 (= C47), G46 (= G48), C48 (= C50), R58 (= R60), C60 (= C62), C100 (= C102), G101 (= G103), C103 (= C105), C137 (= C137), C139 (= C139)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q101), C139 (= C139)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding 3-phenylpropanal: 255, 257, 258, 752
- binding bicarbonate ion: 460, 498, 531, 532, 535, 539, 890, 892
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4us8A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with benzaldehyde (see paper)
40% identity, 88% coverage: 8:160/173 of query aligns to 6:160/907 of 4us8A
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y40), C40 (= C42), E41 (= E43), G43 (= G45), C45 (= C47), G46 (= G48), C48 (= C50), R58 (= R60), C60 (= C62), C100 (= C102), G101 (= G103), C103 (= C105), C137 (= C137), C139 (= C139)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q101), C139 (= C139)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 498, 531, 532, 535, 539
- binding benzaldehyde: 255, 255, 394, 425, 425, 425, 425, 497, 497, 501, 531, 535, 535, 626, 626, 626, 694, 696, 697
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4c7yA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with sodium dithionite and sodium sulfide (see paper)
40% identity, 88% coverage: 8:160/173 of query aligns to 6:160/907 of 4c7yA
- binding fe2/s2 (inorganic) cluster: C40 (= C42), E41 (= E43), G43 (= G45), C45 (= C47), G46 (= G48), C48 (= C50), R58 (= R60), C60 (= C62), C100 (= C102), G101 (= G103), C103 (= C105), C137 (= C137), C139 (= C139)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q101), C139 (= C139)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 498, 531, 535, 539
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
- binding hydrogen peroxide: 696, 697, 869
3fc4A Ethylene glycol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
40% identity, 88% coverage: 8:160/173 of query aligns to 6:160/907 of 3fc4A
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y40), C40 (= C42), E41 (= E43), G43 (= G45), C45 (= C47), G46 (= G48), C48 (= C50), R58 (= R60), C60 (= C62), C100 (= C102), G101 (= G103), C103 (= C105), C137 (= C137), C139 (= C139)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q101), C139 (= C139)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding 1,2-ethanediol: 535, 622, 696, 697, 869
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
3fahA Glycerol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
40% identity, 88% coverage: 8:160/173 of query aligns to 6:160/907 of 3fahA
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y40), C40 (= C42), E41 (= E43), G43 (= G45), C45 (= C47), G46 (= G48), C48 (= C50), R58 (= R60), C60 (= C62), C100 (= C102), G101 (= G103), C103 (= C105), C137 (= C137), C139 (= C139)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q101), C139 (= C139)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding glycerol: 416, 535, 622, 683, 696, 697, 869, 884, 889, 890, 892
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
Query Sequence
>H281DRAFT_02675 FitnessBrowser__Burk376:H281DRAFT_02675
METRTVRVVVNGTAYEREVESRRMLGDFLRHDLELTGTHYGCEHGVCGACTVLIEGRTAR
ACLTLAAQVNGRSLTTVEGLAHADGTLNELQKAFTETHALQCGFCTPGMLMTLTELLNET
DSPSESEIREAITGNLCRCTGYQPIVAAALLAAQRIRDQRAAASTTPAAHATE
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory