SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_02680 H281DRAFT_02680 succinate semialdehyde dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
67% identity, 98% coverage: 11:490/492 of query aligns to 2:481/482 of P25526

query
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P25526

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WN 156:157 (= WN 165:166) binding
KPAS 180:183 (≠ KPAE 189:192) binding
GS 233:234 (= GS 242:243) binding
L256 (= L265) binding
E386 (= E395) binding

3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
67% identity, 98% coverage: 11:490/492 of query aligns to 1:480/481 of 3jz4A

query
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3jz4A

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active site: N156 (= N166), K179 (= K189), E254 (= E264), C288 (= C298), E385 (= E395), E462 (= E472)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I152 (= I162), P154 (= P164), W155 (= W165), N156 (= N166), K179 (= K189), A181 (= A191), S182 (≠ E192), S211 (≠ D221), A212 (≠ P222), G213 (≠ R223), G216 (= G226), F230 (= F240), T231 (= T241), G232 (= G242), S233 (= S243), I236 (≠ V246), E254 (= E264), L255 (= L265), C288 (= C298), K338 (= K348), E385 (= E395), F387 (= F397)

P51649 Succinate-semialdehyde dehydrogenase, mitochondrial; Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase; EC 1.2.1.24 from Homo sapiens (Human) (see 5 papers)
58% identity, 97% coverage: 16:492/492 of query aligns to 57:535/535 of P51649

query
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P51649

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F

A

A

A

E

A

A

V

M

G

K

R

K

-

N

I

L

K

R

V

V

G

G

N
|
N

G

G

F

F

E

E

S

E

G

G

V

T

T

T

Q

Q

G

G

P
|
P

L

L

I

I

N

N

E

E

A

K

A

A

V

V

E

E

K

K

V

V

E

E

A

K

H

Q

I

V

A

N

D

D

A

A

V

V

A

S

H

K

G

G

A

A

R

T

V
|
V

L

V

T

T

G
|
G

G

G

K

K

R

R

H

H

A

Q

A

L

G

G

K

K

L

N

F

F

E

E

P

P

T

T

V

L

G

C

D

N

V

V

T

T

A

Q

R

D

M

M

R

L

F

C

A

T

T

H

E

E

T

T

F

F

G

G

P

P

V

L

A

A

P

P

L

V

F

I

R

K

F

F

T

D

N

T

E

E

R

E

E

E

A

A

I

I

A

A

A

I

A

A

N

N

A

A

T

A

E

D

F

V

G

G

L

L

A

A

A

G

Y

Y

F

F

Y

Y

S

S

R

Q

D

D

I

P

G

A

R

Q

I

I

W

W

R

R

V

V

A

A

E

E

A

Q

L

L

E

E

Y

V

G

G

M

M

V

V

G

G

I

V

N

N

T

E

G

G

L

L

I

I

S
|
S

N

S

E

V

V

E

A

C

P

P

F

F

G

G

V

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E

E

G

G

S

S

K

K

Y

Y

G

G

I

I

E

D

D

E

Y

Y

L

L

E

E

I

L

K

K

Y

Y

L

V

C

C

M

Y

G
|
G

G

G

L

L

C93 (≠ M54) to F: in SSADHD; 3% of activity; dbSNP:rs765561257
G176 (= G137) to R: in SSADHD; <1% of activity; dbSNP:rs72552281
H180 (≠ A141) to Y: 83% of activity; dbSNP:rs2760118
P182 (= P143) to L: 48% of activity; dbSNP:rs3765310
R213 (= R174) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
C223 (= C184) to Y: in SSADHD; 5% of activity; dbSNP:rs72552282
KPAE 228:231 (= KPAE 189:192) binding
T233 (= T194) to M: in SSADHD; 4% of activity; dbSNP:rs1326526453
A237 (= A198) to S: 65% of activity; dbSNP:rs62621664
N255 (≠ S216) to S: in SSADHD; 17% of activity; dbSNP:rs145087265
G268 (= G226) to E: in SSADHD; <1% of activity; dbSNP:rs375628463
GSTTTG 284:289 (≠ GSTPVG 242:247) binding
R334 (= R292) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
N335 (= N293) to K: in SSADHD; 1% of activity; dbSNP:rs72552283
C340 (= C298) modified: Disulfide link with 342, In inhibited form
C342 (= C300) modified: Disulfide link with 340, In inhibited form; mutation to A: Loss of regulation by redox state.
N372 (= N329) natural variant: N -> S
P382 (= P339) to L: in SSADHD; 2% of activity
V406 (= V363) to I: in dbSNP:rs143741652
G409 (= G366) to D: in SSADHD; <1% of activity; dbSNP:rs118203984
S498 (= S455) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
G533 (= G490) to R: in SSADHD; <1% of activity; dbSNP:rs72552284

Sites not aligning to the query:

36 G → R: no effect on succinate-semialdehyde dehydrogenase activity; dbSNP:rs4646832

2w8rA The crystal structure of human ssadh in complex with NAD+ (see paper)
58% identity, 97% coverage: 16:492/492 of query aligns to 7:485/485 of 2w8rA

query
sites
2w8rA

S

A

L

L

R

R

T

T

L

D

A

S

Y

F

I

V

D

G

G

G

Q

R

W

W

C

L

G

P

A

A

D

-

A

A

R

A

T

T

F

F

A

P

V

V

D

Q

D

D

P

P

A

A

T

S

G

G

E

A

K

A

I

L

A

G

D

M

V

V

P

A

L

D

M

C

T

G

G

V

A

R

E

E

T

A

R

R

R

A

A

A

I

V

E

R

A

A

G

A

E

Y

H

E

A

A

Q

F

R

C

G

R

W

W

R

R

K

E

L

V

T

S

A

A

A

K

Q

E

R

R

S

S

T

S

I

L

L

L

K

R

R

K

W

W

H

Y

A

N

L

L

M

M

I

I

A

Q

N

N

T

K

D

D

L

L

A

A

I

R

I

I

M

I

S

T

A

A

E

E

Q

S

G

G

K

K

P

P

L

L

A

K

E

E

A

A

K

H

G

G

E

E

I

I

G

L

Y

Y

A

S

A

A

S

F

F

F

I

L

E

E

W

W

F

F

A

S

E

E

Q

E

A

A

K

R

R

R

V

V

D

Y

G

G

D

D

V

I

L

I

A

H

S

T

P

P

A

A

A

K

D

D

K

R

R

R

M

A

L

L

V

V

T

L

K

K

E

Q

P

P

I

I

G

G

V

V

C

A

A

A

A

V

I
|
I

T
|
T

P
|
P

W
|
W

N
|
N

F

F

P

P

A

S

A

A

M

M

I

I

T

T

R

R

K

K

V

V

A

G

P

A

A

A

L

L

A

A

G

G

C

C

A

T

M

V

I

V

L

V

K
|
K

P
|
P

A
|
A

E
|
E

A

D

T

T

P

P

L

F

S

S

A

A

L

L

A

A

L

L

A

A

E

E

L

L

A

A

H

S

R

Q

A

A

G

G

V

I

P

P

A

S

G

G

V

V

F

Y

S

N

V

V

V

I

-

P

-

C

-

S

V

R

G

K

D

N

P
x
A

R
x
K

S

E

I

V

G

G

A

E

E

A

M

I

T

C

S

T

N

D

P

P

I

L

V

V

R

S

K

K

L

I

S

S

F
|
F

T

T

G

G

S
|
S

T

T

P

T

V
x
T

G

G

R

K

M

I

L

L

M

L

S

H

Q

H

C

A

A

A

P

N

T

S

V

V

K

K

K

R

L

V

S

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

D

N

L

V

D

D

A

Q

A

A

V

V

E

A

G

G

A

A

L

M

A

A

S

S

K

K

Y

F

R

R

N

N

A

T

G

G

Q

Q

T

T

C
x
A

V

V

C

C

T

S

N

N

R

Q

V

F

Y

L

V

V

Q

Q

D

R

G

G

V

I

Y

H

D

D

A

A

F

F

A

V

E

K

K

A

F

F

A

A

A

E

A

A

V

M

G

K

R

K

-

N

I

L

K

R

V

V

G

G

N

N

G

G

F

F

E

E

S

E

G

G

V

T

T

T

Q

Q

G

G

P

P

L

L

I

I

N

N

E

E

A

K

A

A

V

V

E

E

K

K

V

V

E

E

A

K

H

Q

I

V

A

N

D

D

A

A

V

V

A

S

H

K

G

G

A

A

R

T

V

V

L

V

T

T

G

G

K

K

R

R

H

H

A

Q

A

L

G

G

K

K

L

N

F

F

E

E

P

P

T

T

V

L

G

C

D

N

V

V

T

T

A

Q

R

D

M

M

R

L

F

C

A

T

T

H

E

E

E
|
E

T

T

F

F

G

G

P

P

V

L

A

A

P

P

L

V

F

I

R

K

F

F

T

D

N

T

E

E

R

E

E

E

A

A

I

I

A

A

A

I

A

A

N

N

A

A

T

A

E

D

F

V

G

G

L

L

A

A

A

G

Y

Y

F

F

Y

Y

S

S

R

Q

D

D

I

P

G

A

R

Q

I

I

W

W

R

R

V

V

A

A

E

E

A

Q

L

L

E

E

Y

V

G

G

M

M

V

V

G

G

I

V

N

N

T

E

G

G

L

L

I

I

S

S

N

S

E

V

V

E

A

C

P

P

F

F

G

G

V

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

K

K

Y

Y

G

G

I

I

E

D

D

E

Y

Y

L

L

E

E

I

L

K

K

Y

Y

L

V

C

C

M

Y

G

G

L

L

active site: N155 (= N166), K178 (= K189), E256 (= E264), A290 (≠ C298), E388 (= E395), E465 (= E472)
binding adenosine-5'-diphosphate: I151 (= I162), T152 (= T163), P153 (= P164), W154 (= W165), K178 (= K189), P179 (= P190), A180 (= A191), E181 (= E192), A214 (≠ P222), K215 (≠ R223), F232 (= F240), S235 (= S243), T238 (≠ V246)

2w8qA The crystal structure of human ssadh in complex with ssa. (see paper)
58% identity, 97% coverage: 16:492/492 of query aligns to 7:485/485 of 2w8qA

query
sites
2w8qA

S

A

L

L

R

R

T

T

L

D

A

S

Y

F

I

V

D

G

G

G

Q

R

W

W

C

L

G

P

A

A

D

-

A

A

R

A

T

T

F

F

A

P

V

V

D

Q

D

D

P

P

A

A

T

S

G

G

E

A

K

A

I

L

A

G

D

M

V

V

P

A

L

D

M

C

T

G

G

V

A

R

E

E

T

A

R

R

R

A

A

A

I

V

E

R

A

A

G

A

E

Y

H

E

A

A

Q

F

R

C

G

R

W

W

R

R

K

E

L

V

T

S

A

A

A

K

Q

E

R

R

S

S

T

S

I

L

L

L

K

R

R

K

W

W

H

Y

A

N

L

L

M

M

I

I

A

Q

N

N

T

K

D

D

L

L

A

A

I

R

I

I

M

I

S

T

A

A

E

E

Q

S

G

G

K

K

P

P

L

L

A

K

E

E

A

A

K

H

G

G

E

E

I

I

G

L

Y
|
Y

A

S

A

A

S

F

F

F

I

L

E

E

W

W

F

F

A

S

E

E

Q

E

A

A

K

R

R

R

V

V

D

Y

G

G

D

D

V

I

L

I

A

H

S

T

P

P

A

A

A

K

D

D

K

R

R

R

M

A

L

L

V

V

T

L

K

K

E

Q

P

P

I

I

G

G

V

V

C

A

A

A

A

V

I

I

T

T

P

P

W

W

N
|
N

F
|
F

P

P

A

S

A

A

M

M

I

I

T

T

R
|
R

K

K

V

V

A

G

P

A

A

A

L

L

A

A

G

G

C

C

A

T

M

V

I

V

L

V

K
|
K

P

P

A

A

E

E

A

D

T

T

P

P

L

F

S

S

A

A

L

L

A

A

L

L

A

A

E

E

L

L

A

A

H

S

R

Q

A

A

G

G

V

I

P

P

A

S

G

G

V

V

F

Y

S

N

V

V

V

I

-

P

-

C

-

S

V

R

G

K

D

N

P

A

R

K

S

E

I

V

G

G

A

E

E

A

M

I

T

C

S

T

N

D

P

P

I

L

V

V

R

S

K

K

L

I

S

S

F

F

T

T

G

G

S

S

T

T

P

T

V

T

G

G

R

K

M

I

L

L

M

L

S

H

Q

H

C

A

A

A

P

N

T

S

V

V

K

K

K

R

L

V

S

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

D

N

L

V

D

D

A

Q

A

A

V

V

E

A

G

G

A

A

L

M

A

A

S

S

K

K

Y

F

R
|
R

N

N

A

T

G

G

Q

Q

T

T

C
x
A

V
|
V

C

C

T

S

N

N

R

Q

V

F

Y

L

V

V

Q

Q

D

R

G

G

V

I

Y

H

D

D

A

A

F

F

A

V

E

K

K

A

F

F

A

A

A

E

A

A

V

M

G

K

R

K

-

N

I

L

K

R

V

V

G

G

N

N

G

G

F

F

E

E

S

E

G

G

V

T

T

T

Q

Q

G

G

P

P

L

L

I

I

N

N

E

E

A

K

A

A

V

V

E

E

K

K

V

V

E

E

A

K

H

Q

I

V

A

N

D

D

A

A

V

V

A

S

H

K

G

G

A

A

R

T

V

V

L

V

T

T

G

G

K

K

R

R

H

H

A

Q

A

L

G

G

K

K

L

N

F

F

E

E

P

P

T

T

V

L

G

C

D

N

V

V

T

T

A

Q

R

D

M

M

R

L

F

C

A

T

T

H

E

E

E
|
E

T

T

F

F

G

G

P

P

V

L

A

A

P

P

L

V

F

I

R

K

F

F

T

D

N

T

E

E

R

E

E

E

A

A

I

I

A

A

A

I

A

A

N

N

A

A

T

A

E

D

F

V

G

G

L

L

A

A

A

G

Y

Y

F

F

Y

Y

S

S

R

Q

D

D

I

P

G

A

R

Q

I

I

W

W

R

R

V

V

A

A

E

E

A

Q

L

L

E

E

Y

V

G

G

M

M

V

V

G

G

I

V

N

N

T

E

G

G

L

L

I

I

S
|
S

N

S

E

V

V

E

A

C

P

P

F
|
F

G

G

V

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

K

K

Y

Y

G

G

I

I

E

D

D

E

Y

Y

L

L

E

E

I

L

K

K

Y

Y

L

V

C

C

M

Y

G

G

L

L

active site: N155 (= N166), K178 (= K189), E256 (= E264), A290 (≠ C298), E388 (= E395), E465 (= E472)
binding succinic acid: Y109 (= Y120), F156 (= F167), R163 (= R174), E256 (= E264), R284 (= R292), A290 (≠ C298), V291 (= V299), S448 (= S455), F454 (= F461)

6j76A Structure of 3,6-anhydro-l-galactose dehydrogenase in complex with nap (see paper)
39% identity, 94% coverage: 23:485/492 of query aligns to 5:471/477 of 6j76A

query
sites
6j76A

Y

Y

I

V

D

G

G

G

Q

E

W

W

C

I

G

D

A

A

D

S

D

N

A

G

R

Q

T

T

F

E

A

P

V

V

D

V

D

S

P

P

A

I

T

N

G

E

E

E

K

V

I

L

A

A

D

Y

V

I

P

Q

L

D

M

A

T

D

G

A

A

S

E

D

T

A

R

E

R

R

A

V

I

L

E

A

A

V

G

A

E

T

H

T

A

A

Q

Q

R

K

G

E

W

W

R

A

K

K

L

Q

T

P

A

A

A

R

Q

Q

R

R

S

A

T

E

I

V

L

L

K

R

R

K

W

F

H

A

A

Q

L

L

M

I

I

R

A

D

N

N

T

K

D

Q

D

Y

L

L

A

A

I

E

I

L

M

L

S

V

A

K

E

E

Q

Q

G

G

K

K

P

L

L

L

A

K

E

V

A

A

K

L

G

G

E

E

I

V

G

E

Y

A

A

T

A

S

S

T

F

F

I

I

E

E

W

Y

F

A

A

C

E

D

Q

W

A

A

K

R

R

Q

V

M

D

D

G

G

D

D

V

I

L

V

A

K

S

S

P

D

A

N

A

A

D

N

K

E

R

Q

M

I

L

M

V

I

T

H

K

K

E

I

P

P

I

R

G

G

V

V

C

V

A

V

A

A

I
|
I

T
|
T

P
x
A

W
|
W

N
|
N

F

F

P

P

A

L

A

A

M

L

I

A

T

G

R

R

K

K

V

I

A

G

P

P

A

A

L

L

A

V

A

A

G

G

C

N

A

S

M

I

I

V

L

V

K
|
K

P
|
P

A
x
T

E
x
S

A

E

T

T

P

P

L

L

S

A

A

T

L

L

A

E

L

L

A

G

E

Y

L

L

A

A

H

E

R

Q

A

A

G

G

V

I

P

P

A

A

G

G

V

V

F

L

S

N

V

I

V

V

V

T

G

G

D
x
G

P
x
G

R
|
R

S

T

I

L

G
|
G

A
x
N

E

E

M

L

T

V

S

G

N

H

P

R

I

M

V

T

R

N

K

M

L

V

S

S

F

M

T
|
T

G
|
G

S
|
S

T

T

P

P

V
x
A

G

G

R

Q

M
x
S

L
x
I

M

I

S

R

Q

A

C

S

A

A

P

N

T

N

V

M

K

A

K

H

L

V

S

Q

L

L

E
|
E

L
|
L

G
|
G

G

G

N

K

A

A

P

P

F

F

I

I

V

V

F

M

D

E

D

D

A

A

D

D

L

L

D

E

A

Q

A

A

V

A

E

A

G

A

A

A

L

L

A

H

S

S

K

R

Y

F

R

D

N

N

A

C

G

G

Q

Q

T

V

C
|
C

V

T

C

C

T

N

N

E

R

R

V

M

Y

Y

V

V

Q

H

D

G

G

A

V

V

Y

Y

D

D

A

E

F

F

A

M

E

R

K

I

F

F

A

M

A

G

A

K

V

V

G

E

R

A

I

I

K

K

V

V

G

G

N

D

G

P

F

M

E

D

S

P

G

A

V

S

T

D

Q

M

G

G

P

P

L

K

I

V

N

N

E

A

A

N

A

E

V

L

E

A

K

H

V

M

E

E

A

E

H

L

I

V

A

A

D

E

A

A

V

V

A

D

H

E

G

G

A

A

R

T

V

V

L

L

T

F

G

G

K

K

R

K

H

L

A

E

A

G

G

P

K

E

-

F

-

E

-

K

-

G

L

F

F

W

F

F

E

E

P

P

T

T

V

V

V

L

G

T

D

N

V

V

T

T

A

Q

R

D

M

M

R

T

F

I

A

V

T

H

E

E

E
|
E

T

S

F
|
F

G

G

P

P

V

I

A

L

P

P

L

V

F

I

R

K

F

F

T

D

N

S

E

F

R

D

E

E

A

V

I

I

A

E

A

Y

A

A

N

N

A

D

T

S

E

D

F

Y

G

G

L

L

A

A

Y

M

F

I

Y

C

S

T

R

Q

D

N

I

M

G

H

R

Y

I

I

W

N

R

R

V

L

A

L

E

T

A

E

L

L

E

E

Y

S

G

G

M

E

V

I

G

Y

I

V

N

N

T

R

G

G

L

H

I

G

S

E

N

Q

E

H

V

Q

A

G

P

F

F
x
H

G

N

G

G

V

Y

K

K

Q

L

S

S

G

G

L

T

G

G

R

G

E
|
E

G

D

S

G

K

K

Y

Y

G

G

I

F

E

E

D

Q

Y

Y

L

L

E

E

I

K

K

K

active site: N148 (= N166), E246 (= E264), C280 (= C298), E458 (= E472)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I144 (= I162), T145 (= T163), A146 (≠ P164), W147 (= W165), N148 (= N166), K171 (= K189), P172 (= P190), T173 (≠ A191), S174 (≠ E192), G203 (≠ D221), G204 (≠ P222), R205 (= R223), G208 (= G226), N209 (≠ A227), T223 (= T241), G224 (= G242), S225 (= S243), A228 (≠ V246), S231 (≠ M249), I232 (≠ L250), E246 (= E264), L247 (= L265), G248 (= G266), C280 (= C298), E381 (= E395), F383 (= F397), H447 (≠ F461)

5x5uA Crystal structure of alpha-ketoglutarate-semialdehyde dehydrogenase (kgsadh) complexed with NAD (see paper)
40% identity, 96% coverage: 20:489/492 of query aligns to 5:474/476 of 5x5uA

query
sites
5x5uA

T

T

L

Q

A

L

Y

L

I

I

D

D

G

G

Q

E

W

W

C

V

G
x
D

A
|
A

D
x
A

D

S

A
x
G

R

K

T

T

F

I

A

D

V

V

D

V

D

N

P

P

A

A

T

T

G

G

E

K

K

P

I

I

A

G

D

R

V

V

P

A

L

H

M

A

T

G

G

I

A

A

E

D

T

L

R

D

R

R

A

A

I

L

E

A

A

A

G

A

E

Q

H

S

A

G

Q

F

R

E

G

A

W

W

R

R

K

K

L

V

T

P

A

A

A

H

Q

E

R

R

S

A

T

A

I

T

L

M

K

R

R

K

W

A

H

A

A

A

L

L

M

V

I

R

A

E

N

R

T

A

D

D

D

A

L

I

A

A

I

Q

I

L

M

M

S

T

A

Q

E

E

Q

Q

G

G

K

K

P

P

L

L

A

T

E

E

A

A

K

R

G

V

E

E

I

V

G

L

Y

S

A

A

S

D

F

I

I

I

E

E

W

W

F

F

A

A

E

D

Q

E

A

G

K

R

R

R

V

V

D

Y

G

G

D

R

V

I

L

V

A

P

S

P

P

R

A

N

A

L

D

G

K

A

R

Q

M

Q

L

T

V

V

T

V

K

K

E

E

P

P

I

V

G

G

V

P

C

V

A

A

I

F

T

T

P
|
P

W
|
W

N
|
N

F

F

P

P

A

V

A

N

M

Q

I

V

T

V

R

R

K

K

V

L

A

S

P

A

A

A

L

L

A

A

A

T

G

G

C

C

A

S

M

F

I

L

L

V

K
|
K

P

A

A

P

E

E

A

E

T

T

P

P

L

A

S

S

A

P

L

A

A

A

L

L

A

L

E

R

L

A

A

F

H

V

R

D

A

A

G

G

V

V

P

P

A

A

G

G

V

V

F

I

S

G

V

L

V

V

V

Y

G

G

D

D

P
|
P

R
x
A

S

E

I

I

G
x
S

A

S

E

Y

M

L

T

I

S

P

N

H

P

P

I

V

V

I

R

R

K

K

L

V

S

T

F
|
F

T
|
T

G
|
G

S
|
S

T

T

P

P

V
|
V

G

G

R

K

M

Q

L
|
L

M

A

S

S

Q

L

C

A

A

G

P

L

T

H

V

M

K

K

K

R

L

A

S

T

L

M

E
|
E

L
|
L

G

G

N

H

A

A

P

P

F

V

I

I

V

V

F

A

D

E

D

D

A

A

D

D

L

V

D

A

A

L

A

A

V

V

E

K

G

A

A

A

L

G

A

G

S

A

K

K

Y

F

R

R

N

N

A

A

G

G

Q

Q

T

V

C
|
C

V

I

C

S

T

P

N

T

R

R

V

F

Y

L

V

V

Q

H

D

N

G

S

V

I

Y

R

D

D

A

E

F

F

A

T

E

R

K

A

F

L

A

V

A

K

A

H

V

A

G

E

R

G

I

L

K

K

V

V

G

G

N

N

G

G

F

L

E

E

S

E

G

G

V

T

T

T

Q

L

G

G

P

A

L

L

I

A

N

N

E

P

A
x
R

V

L

E

T

K

A

V

M

E

A

A

S

H

V

I

I

A

D

D

N

A

A

V

R

A

K

H

V

G

G

A

A

R

S

V

I

L

E

T

T

G

G

K

E

R

R

H

I

A

G

A

S

G

E

K

G

L

N

F

F

E

A

P

P

T

T

V

V

V

I

G

A

D

N

V

V

T

P

A

L

R

D

M

A

R

D

F

V

A

F

T

N

E

N

E
|
E

T

P

F
|
F

G

G

P

P

V

V

A

A

P

A

L

I

F

R

R

G

F

F

T

D

N

K

E

L

R

E

E

E

A

A

I

I

A

A

A

E

A

A

N

N

A

R

T

L

E

P

F

F

G

G

L

L

A

A

A

G

Y

Y

F

A

Y

F

S

T

R

R

D

S

I

F

G

A

R

N

I

V

W

H

R

L

V

L

A

T

E

Q

A

R

L

L

E

E

Y

V

G

G

M

M

V

L

G

W

I

I

N

N

T

Q

G

P

L

A

I

T

S

P

N

W

E

P

V

E

A

M

P

P

F

F

G

G

V

V

K

K

Q

D

S

S

G

G

L

Y

G

G

R

S

E
|
E

G

G

S

G

K

P

Y

E

G

A

I

L

E

E

D

P

Y

Y

L

L

E

V

I

T

K

K

Y

S

L

V

C

T

M

V

active site: N151 (= N166), K174 (= K189), E249 (= E264), C283 (= C298), E380 (= E395), E457 (= E472)
binding glycerol: D15 (≠ G30), A16 (= A31), A17 (≠ D32), G19 (≠ A34)
binding nicotinamide-adenine-dinucleotide: P149 (= P164), W150 (= W165), N151 (= N166), P207 (= P222), A208 (≠ R223), S211 (≠ G226), F225 (= F240), T226 (= T241), G227 (= G242), S228 (= S243), V231 (= V246), L235 (= L250), E249 (= E264), L250 (= L265), C283 (= C298), R329 (≠ A344), R330 (≠ A345), E380 (= E395), F382 (= F397)

5x5tA Crystal structure of alpha-ketoglutarate semialdehyde dehydrogenase (kgsadh) from azospirillum brasilense (see paper)
40% identity, 96% coverage: 20:489/492 of query aligns to 5:474/476 of 5x5tA

query
sites
5x5tA

T

T

L

Q

A

L

Y

L

I

I

D

D

G

G

Q

E

W

W

C

V

G

D

A

A

D

A

D

S

A

G

R

K

T

T

F

I

A

D

V

V

D

V

D

N

P

P

A

A

T

T

G

G

E

K

K

P

I

I

A

G

D

R

V

V

P

A

L

H

M

A

T

G

G

I

A

A

E

D

T

L

R

D

R

R

A

A

I

L

E

A

A

A

G

A

E

Q

H

S

A

G

Q

F

R

E

G

A

W

W

R

R

K

K

L

V

T

P

A

A

A

H

Q

E

R

R

S

A

T

A

I

T

L

M

K

R

R

K

W

A

H

A

A

A

L

L

M

V

I

R

A

E

N

R

T

A

D

D

D

A

L

I

A

A

I

Q

I

L

M

M

S

T

A

Q

E

E

Q

Q

G

G

K

K

P

P

L

L

A

T

E

E

A

A

K

R

G

V

E

E

I

V

G

L

Y

S

A

A

S

D

F

I

I

I

E

E

W

W

F

F

A

A

E

D

Q

E

A

G

K

R

R

R

V

V

D

Y

G

G

D

R

V

I

L

V

A

P

S

P

P

R

A

N

A

L

D

G

K

A

R

Q

M

Q

L

T

V

V

T

V

K

K

E

E

P

P

I

V

G

G

V

P

C

V

A

A

I

F

T

T

P

P

W

W

N
|
N

F

F

P

P

A

V

A

N

M

Q

I

V

T

V

R

R

K

K

V

L

A

S

P

A

A

A

L

L

A

A

A

T

G

G

C

C

A

S

M

F

I

L

L

V

K
|
K

P

A

A

P

E

E

A

E

T

T

P

P

L

A

S

S

A

P

L

A

A

A

L

L

A

L

E

R

L

A

A

F

H

V

R

D

A

A

G

G

V

V

P

P

A

A

G

G

V

V

F

I

S

G

V

L

V

V

V

Y

G

G

D

D

P

P

R

A

S

E

I

I

G

S

A

S

E

Y

M

L

T

I

S

P

N

H

P

P

I

V

V

I

R

R

K

K

L

V

S

T

F

F

T

T

G

G

S

S

T

T

P

P

V

V

G

G

R

K

M

Q

L

L

M
x
A

S

S

Q

L

C
x
A

A
x
G

P

L

T

H

V

M

K

K

K

R

L

A

S

T

L

M

E
|
E

L

L

G

G

N

H

A

A

P

P

F

V

I

I

V

V

F

A

D

E

D

D

A

A

D

D

L

V

D

A

A

L

A

A

V

V

E

K

G

A

A

A

L

G

A

G

S

A

K

K

Y

F

R

R

N

N

A

A

G

G

Q

Q

T

V

C
|
C

V

I

C

S

T

P

N

T

R

R

V

F

Y

L

V

V

Q

H

D

N

G

S

V

I

Y

R

D

D

A

E

F

F

A

T

E

R

K

A

F

L

A

V

A

K

A

H

V

A

G

E

R

G

I

L

K

K

V

V

G

G

N

N

G

G

F

L

E

E

S

E

G

G

V

T

T

T

Q

L

G

G

P

A

L

L

I

A

N

N

E

P

A

R

V

L

E

T

K

A

V

M

E

A

A

S

H

V

I

I

A

D

D

N

A

A

V

R

A

K

H

V

G

G

A

A

R

S

V

I

L

E

T

T

G

G

K

E

R

R

H

I

A

G

A

S

G

E

K

G

L

N

F

F

E

A

P

P

T

T

V

V

V

I

G

A

D

N

V

V

T

P

A

L

R

D

M

A

R

D

F

V

A

F

T

N

E

N

E
|
E

T

P

F

F

G

G

P

P

V

V

A

A

P

A

L

I

F

R

R

G

F

F

T

D

N

K

E

L

R

E

E

E

A

A

I

I

A

A

A

E

A

A

N

N

A

R

T

L

E

P

F

F

G

G

L

L

A

A

A

G

Y

Y

F

A

Y

F

S

T

R

R

D

S

I

F

G

A

R

N

I

V

W

H

R

L

V

L

A

T

E

Q

A

R

L

L

E

E

Y

V

G

G

M

M

V

L

G

W

I

I

N

N

T

Q

G

P

L

A

I

T

S

P

N

W

E

P

V

E

A

M

P

P

F

F

G

G

V

V

K

K

Q

D

S

S

G

G

L
x
Y

G

G

R

S

E
|
E

G

G

S

G

K

P

Y

E

G

A

I

L

E

E

D

P

Y

Y

L

L

E

V

I

T

K

K

Y

S

L

V

C

T

M

V

active site: N151 (= N166), K174 (= K189), E249 (= E264), C283 (= C298), E380 (= E395), E457 (= E472)
binding glycerol: A236 (≠ M251), A239 (≠ C254), G240 (≠ A255), Y454 (≠ L469)

6wsbA Crystal structure of a betaine aldehyde dehydrogenase from burkholderia pseudomallei bound to cofactor NAD (see paper)
44% identity, 94% coverage: 23:485/492 of query aligns to 9:475/489 of 6wsbA

query
sites
6wsbA

Y

Y

I

I

D

A

G

G

Q

A

W

H

C

A

G

D

A

A

D

T

D

S

A

G

R

K

T

T

F

F

A

D

V

T

D

F

D

D

P

P

A

A

T

T

G

G

E

E

K

L

I

L

A

A

D

R

V

V

P

Q

L

Q

M

A

T

S

G

A

A

D

E

D

T

V

R

D

R

R

A

A

I

V

E

A

A

S

G

A

E

R

H

E

A

G

Q

Q

R

R

G

E

W

W

R

A

K

A

L

M

T

T

A

A

A

M

Q

Q

R

R

S

S

T

R

I

I

L

L

K

R

R

R

W

A

H

V

A

E

L

L

M

L

I

R

A

E

N

R

T

N

D

D

D

A

L

L

A

A

I

E

I

L

M

E

S

M

A

R

E

D

Q

T

G

G

K

K

P

P

L

I

A

A

E

E

A

T

K

R

G

A

-

V

E

D

I

I

G

V

Y

T

A

G

A

A

S

D

F

V

I

I

E

E

W

Y

F

Y

A

A

E

G

Q

L

A

A

K

T

R

A

V

I

D

E

G

G

D

-

V

-

L

L

A

Q

S

V

P

P

A

L

A

R

D

P

K

E

R

S

M

F

L

V

V

Y

T

T

K

R

-

R

E

E

P

P

I

L

G

G

V

V

C

C

A

A

A

G

I
|
I

T
x
G

P
x
A

W
|
W

N
|
N

F

Y

P

P

A

I

A

Q

M

I

I

A

T

C

R

W

K

K

V

S

A

A

P

P

A

A

L

L

A

A

G

G

C

N

A

A

M

M

I

I

L

F

K
|
K

P
|
P

A
x
S

E
|
E

A

V

T

T

P

P

L

L

S

S

A

A

L

L

A

K

L

L

A

A

E

E

L

I

A

Y

H

T

R

E

A

A

G

G

V

V

P

P

A

A

G

G

V

V

F

F

S

N

V

V

V

Q

G

G

D
|
D

P
x
G

R

-

S

S

I

V

G
|
G

A
|
A

E

L

M

L

T

S

S

A

N

H

P

P

I

G

V

I

R

A

K

K

L

V

S

S

F
|
F

T
|
T

G
|
G

S
x
G

T

V

P

E

V
x
T

G

G

R

K

M
x
K

L
x
V

M

M

S

S

Q

L

C

A

-

G

A

A

P

S

T

S

V

L

K

K

K

E

L

V

S

T

L

M

E
|
E

L
|
L

G
|
G

G

G

N

K

A

S

P

P

F

L

I

I

V

V

F

F

D

D

A

A

D

D

L

L

D

D

A

R

A

A

V

A

E

D

G

I

A

A

L

V

A

T

S

A

K

N

Y

F

R

F

N

S

A

A

G

G

Q

Q

T

V

C
|
C

V

T

C

N

T

G

N

T

R

R

V

V

Y

F

V

V

Q

Q

D

Q

G

A

V

V

Y

K

D

D

A

A

F

F

A

V

E

E

K

R

F

V

A

L

A

A

A

R

V

V

G

A

R

R

I

I

K

R

V

V

G

G

N

K

G

P

F

S

E

D

S

S

G

D

V

T

T

N

Q

F

G

G

P

P

L

L

I

A

N

S

E

A

A

A

A

Q

V

L

E

D

K

K

V

V

E

L

A

G

H

Y

I

I

A

D

D

S

A

G

V

K

A

A

H

E

G

G

A

A

R

K

V

L

L

L

T

A

G

G

K

A

R

R

-

L

-

V

-

N

-

D

H

H

A

F

A

A

G

S

K

G

L

Q

F

Y

F

V

E

A

P

P

T

T

V

V

V

F

G

G

D

D

V

C

T

R

A

D

R

D

M

M

R

R

F

I

A

V

T

R

E

E

E
|
E

T

I

F
|
F

G

G

P

P

V

V

A

M

P

S

L

I

F

L

R

S

F

F

T

E

N

T

E

E

R

D

E

E

A

A

I

I

A

A

A

R

A

A

N

N

A

A

T

T

E

D

F

Y

G

G

L

L

A

A

Y

G

F

V

Y

V

S

T

R

E

D

N

I

L

G

S

R

R

I

A

W

H

R

R

V

A

A

I

E

H

A

R

L

L

E

E

Y

A

G

G

M

I

V

C

G

W

I

I

N

N

T

T

G

W

L

G

I

E

S

S

N

P

E

A

V

E

A

M

P

P

F

V

G

G

V

Y

K

K

Q

Q

S

S

G

G

L

V

G

G

R

R

E
|
E

G

N

S

G

K

I

Y

T

G

T

I

L

E

E

D

H

Y

Y

L

T

E

R

I

I

K

K

active site: N152 (= N166), E250 (= E264), C284 (= C298), E462 (= E472)
binding nicotinamide-adenine-dinucleotide: I148 (= I162), G149 (≠ T163), A150 (≠ P164), W151 (= W165), N152 (= N166), K175 (= K189), P176 (= P190), S177 (≠ A191), E178 (= E192), D207 (= D221), G208 (≠ P222), G211 (= G226), A212 (= A227), F225 (= F240), T226 (= T241), G227 (= G242), G228 (≠ S243), T231 (≠ V246), K234 (≠ M249), V235 (≠ L250), E250 (= E264), L251 (= L265), G252 (= G266), C284 (= C298), E385 (= E395), F387 (= F397)

P17202 Aminoaldehyde dehydrogenase BADH; 4-trimethylammoniobutyraldehyde dehydrogenase BADH; Aminobutyraldehyde dehydrogenase BADH; Betaine aldehyde dehydrogenase; SoBADH; EC 1.2.1.-; EC 1.2.1.47; EC 1.2.1.19; EC 1.2.1.8 from Spinacia oleracea (Spinach) (see 3 papers)
37% identity, 97% coverage: 12:487/492 of query aligns to 1:482/497 of P17202

query
sites
P17202

L

M

A

A

D

F

P

P

S

I

L

P

L

A

R

R

T

Q

L

L

A

-

Y

F

I

I

D

D

G

G

Q

E

W

W

C

R

G

E

A

P

D

I

D

K

A

K

R

N

T

R

F

I

A

P

V

V

D
x
I

D

N

P

P

A

S

T

T

G

E

E

E

K

I

I

I

A

G

D

D

V

I

P

P

L

A

M

A

T

T

G

A

A

E

E

D

T

V

R

E

R

V

A

A

I

V

E

V

A

A

G

A

E

R

H

R

A

A

Q

F

R

R

-

R

-

N

G

N

W

W

R

S

K

A

L

T

T

S

A

G

A

A

Q

H

R

R

S

A

T

T

I

Y

L

L

K

R

R

A

W

I

H

A

A

A