SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing H281DRAFT_02886 FitnessBrowser__Burk376:H281DRAFT_02886 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1rqxC Crystal structure of acc deaminase complexed with inhibitor (see paper)
95% identity, 100% coverage: 1:338/338 of query aligns to 1:338/338 of 1rqxC

query
sites
1rqxC

M

M

N

N

L

L

Q

Q

R

R

F

F

P

P

R

R

Y

Y

P

P

L

L

T

T

F

F

G

G

P

P

T

T

P

P

I

I

Q

Q

P

P

L

L

K

A

R

R

L

L

S

S

D

K

H

H

L

L

G

G

G

G

K

K

V

V

H

H

L

L

Y

Y

A

A

K

K

R

R

E

E

D

D

C

C

N

N

S

S

G

G

F

L

A

A

F

F

G

G

G

G

N
|
N

K
|
K

T

T

R

R

K
|
K

L

L

E

E

Y

Y

L

L

I

I

P

P

E

E

A

A

L

L

A

A

Q

Q

G

G

C

C

D

D

T

T

L

L

V

V

S

S

I

I

G

G

G

G

I

I

Q

Q

S
|
S

N
|
N

Q
|
Q

T

T

R

R

Q

Q

V

V

A

A

A

A

V

V

A

A

A

A

H

H

L

L

G

G

M

M

K

K

C

C

V

V

L

L

V

V

Q

Q

E

E

N

N

W

W

V

V

N

N

Y

Y

S

S

D

D

A

A

V

V

Y

Y

D

D

R

R

V

V

G

G

N

N

I

I

Q

Q

M

M

S

S

R

R

I

I

L

L

G

G

A

A

D

D

V

V

R

R

L

L

V

V

P

P

D

D

G

G

F

F

D

D

I

I

G

G

F

F

R

R

K

R

S

S

W

W

E

E

D

D

A

A

L

L

E

E

S

S

V

V

R

R

A

A

A

A

G

G

G

G

K

K

P

P

Y

Y

A

A

I

I

P

P

A

A

G

G

C

C

S

S

D

D

H

H

P

P

L

L

G

G

G

G

L

L

G

G

F

F

V

V

G

G

F

F

A

A

E

E

E

E

V

V

R

R

Q

A

Q

Q

E

E

A

A

E

E

L

L

G

G

F

F

K

K

F

F

D

D

Y

Y

I

V

V

V

V

V

C

C

S
|
S

V
|
V

T
|
T

G
|
G

S

S

T
|
T

Q

Q

A

A

G

G

M

M

V

V

V

V

G

G

F

F

A

A

A

A

D

D

G

G

R

R

A

A

D

D

R

R

V

V

I

I

G

G

I

V

D

D

A

A

S

S

A

A

K

K

P

P

A

A

Q

Q

T

T

R

R

E

E

Q

Q

I

I

T

T

R

R

I

I

A

A

S

R

R

Q

T

T

A

A

E

E

K

K

V

V

G

G

L

L

G

E

R

R

D

D

I

I

T

M

A

R

N

A

D

D

V

V

V

V

L

L

D

D

E

E

R

R

F

F

G

A

G

G

P

P

E

E

Y
|
Y

G

G

L

L

P

P

N

N

D

E

G

G

T

T

L

L

E

E

A

A

I

I

R

R

L

L

C

C

A

A

R

R

L

T

E

E

G

G

V

M

L

L

T

T

D

D

P

P

V

V

Y
|
Y

E
|
E

G

G

K

K

S

S

M

M

H

H

G

G

M

M

I

I

D

E

M

M

V

V

R

R

N

N

G

G

E

E

F

F

P

P

E

E

G

G

S

S

R

R

V

V

L

L

Y

Y

A

A

H

H

L
|
L

G
|
G

G
|
G

V

V

P

P

A

A

L

L

N

N

G

G

Y

Y

S

S

F

F

I

I

F

F

R

R

D

D

G

G

active site: K51 (= K51), Y268 (= Y268), Y294 (= Y294)
binding 1-aminocyclopropylphosphonate: K51 (= K51), S78 (= S78), N79 (= N79), Q80 (= Q80), T199 (= T199), Y294 (= Y294)
binding pyridoxal-5'-phosphate: N50 (= N50), K51 (= K51), K54 (= K54), N79 (= N79), S197 (= S197), V198 (= V198), T199 (= T199), G200 (= G200), T202 (= T202), Y294 (= Y294), E295 (= E295), L322 (= L322), G323 (= G323), G324 (= G324)

1tzmA Crystal structure of acc deaminase complexed with substrate analog b- chloro-d-alanine (see paper)
93% identity, 100% coverage: 1:338/338 of query aligns to 1:331/331 of 1tzmA

query
sites
1tzmA

M

M

N

N

L

L

Q

Q

R

R

F

F

P

P

R

R

Y

Y

P

P

L

L

T

T

F

F

G

G

P

P

T

T

P

P

I

I

Q

Q

P

P

L

L

K

A

R

R

L

L

S

S

D

K

H

H

L

L

G

G

G

G

K

K

V

V

H

H

L

L

Y

Y

A

A

K

K

R

R

E

E

D

D

C

C

N

N

S

S

G

G

F

L

A

A

F

F

G

G

G

G

N
|
N

K
|
K

T

T

R

R

K
|
K

L

L

E

E

Y

Y

L

L

I

I

P

P

E

E

A

A

L

L

A

A

Q

Q

G

G

C

C

D

D

T

T

L

L

V

V

S

S

I

I

G
|
G

G

G

I

I

Q

Q

S
|
S

N
|
N

Q
|
Q

T

T

R

R

Q

Q

V

V

A

A

A

A

V

V

A

A

A

A

H

H

L

L

G

G

M

M

K

K

C

C

V

V

L

L

V

V

Q

Q

E

E

N

N

W
|
W

V

V

N

N

Y

Y

S

S

D

D

A

A

V

V

Y

Y

D

D

R

R

V

V

G

G

N

N

I

I

Q

Q

M

M

S

S

R

R

I

I

L

L

G

G

A

A

D

D

V

V

R

R

L

L

V

V

P

P

D

D

G

-

F

-

D

-

I

-

G

-

F

-

R

-

K

R

S

S

W

W

E

E

D

D

A

A

L

L

E

E

S

S

V

V

R

R

A

A

A

A

G

G

G

G

K

K

P

P

Y

Y

A

A

I

I

P

P

A
|
A

G
|
G

C

C

S

S

D

D

H

H

P

P

L

L

G

G

G

G

L

L

G

G

F

F

V

V

G

G

F

F

A

A

E

E

E

E

V

V

R

R

Q

A

Q

Q

E

E

A

A

E

E

L

L

G

G

F

F

K

K

F

F

D

D

Y

Y

I

V

V

V

V

V

C
|
C

S
|
S

V
|
V

T
|
T

G
|
G

S

S

T
|
T

Q

Q

A

A

G

G

M

M

V

V

V

V

G

G

F

F

A

A

A

A

D

D

G

G

R

R

A

A

D

D

R

R

V

V

I

I

G

G

I

V

D

D

A

A

S

S

A

A

K

K

P

P

A

A

Q

Q

T

T

R

R

E

E

Q

Q

I

I

T

T

R

R

I

I

A

A

S

R

R

Q

T

T

A

A

E

E

K

K

V

V

G

G

L

L

G

E

R

R

D

D

I

I

T

M

A

R

N

A

D

D

V

V

V

V

L

L

D

D

E

E

R

R

F

F

G

A

G

G

P

P

E

E

Y
|
Y

G

G

L

L

P

P

N

N

D

E

G

G

T

T

L

L

E

E

A

A

I

I

R

R

L

L

C

C

A

A

R

R

L

T

E

E

G

G

V

M

L

L

T

T

D

D

P

P

V

V

Y
|
Y

E
|
E

G

G

K

K

S

S

M

M

H

H

G

G

M

M

I

I

D

E

M

M

V

V

R

R

N

N

G

G

E

E

F

F

P

P

E

E

G

G

S

S

R

R

V

V

L

L

Y

Y

A

A

H

H

L
|
L

G
|
G

G
|
G

V

V

P

P

A

A

L

L

N

N

G

G

Y

Y

S

S

F

F

I

I

F

F

R

R

D

D

G

G

active site: K51 (= K51), Y261 (= Y268), Y287 (= Y294)
binding 3-chloro-D-alanine: G74 (= G74), S78 (= S78), N79 (= N79), Q80 (= Q80), W102 (= W102), A153 (= A160), G154 (= G161), Y287 (= Y294)
binding amino-acrylate: K51 (= K51), S78 (= S78), Q80 (= Q80), Y287 (= Y294)
binding pyridoxal-5'-phosphate: N50 (= N50), K51 (= K51), K54 (= K54), N79 (= N79), C189 (= C196), S190 (= S197), V191 (= V198), T192 (= T199), G193 (= G200), T195 (= T202), Y287 (= Y294), E288 (= E295), L315 (= L322), G316 (= G323), G317 (= G324)

1tzkA Crystal structure of 1-aminocyclopropane-1-carboxylate-deaminase complexed with alpha-keto-butyrate (see paper)
93% identity, 100% coverage: 1:338/338 of query aligns to 1:331/331 of 1tzkA

query
sites
1tzkA

M

M

N

N

L

L

Q

Q

R

R

F

F

P

P

R

R

Y

Y

P

P

L

L

T

T

F

F

G

G

P

P

T

T

P

P

I

I

Q

Q

P

P

L

L

K

A

R

R

L

L

S

S

D

K

H

H

L

L

G

G

G

G

K

K

V

V

H

H

L

L

Y

Y

A

A

K

K

R

R

E

E

D

D

C

C

N

N

S

S

G

G

F

L

A

A

F

F

G

G

G

G

N
|
N

K
|
K

T

T

R

R

K
|
K

L

L

E

E

Y

Y

L

L

I

I

P

P

E

E

A

A

L

L

A

A

Q

Q

G

G

C

C

D

D

T

T

L

L

V

V

S

S

I

I

G
|
G

G

G

I

I

Q

Q

S
|
S

N
|
N

Q
|
Q

T

T

R

R

Q

Q

V

V

A

A

A

A

V

V

A

A

A

A

H

H

L

L

G

G

M

M

K

K

C

C

V

V

L

L

V

V

Q

Q

E

E

N

N

W

W

V

V

N

N

Y

Y

S

S

D

D

A

A

V

V

Y

Y

D

D

R

R

V

V

G

G

N

N

I

I

Q

Q

M

M

S

S

R

R

I

I

L

L

G

G

A

A

D

D

V

V

R

R

L

L

V

V

P

P

D

D

G

-

F

-

D

-

I

-

G

-

F

-

R

-

K

R

S

S

W

W

E

E

D

D

A

A

L

L

E

E

S

S

V

V

R

R

A

A

A

A

G

G

G

G

K

K

P

P

Y

Y

A

A

I

I

P

P

A
|
A

G
|
G

C

C

S

S

D

D

H

H

P

P

L

L

G

G

G

G

L

L

G

G

F

F

V

V

G

G

F

F

A

A

E

E

E

E

V

V

R

R

Q

A

Q

Q

E

E

A

A

E

E

L

L

G

G

F

F

K

K

F

F

D

D

Y

Y

I

V

V

V

V

V

C
|
C

S
|
S

V
|
V

T
|
T

G
|
G

S

S

T
|
T

Q

Q

A

A

G

G

M

M

V

V

V

V

G

G

F

F

A

A

A

A

D

D

G

G

R

R

A

A

D

D

R

R

V

V

I

I

G

G

I

V

D

D

A

A

S

S

A

A

K

K

P

P

A

A

Q

Q

T

T

R

R

E

E

Q

Q

I

I

T

T

R

R

I

I

A

A

S

R

R

Q

T

T

A

A

E

E

K

K

V

V

G

G

L

L

G

E

R

R

D

D

I

I

T

M

A

R

N

A

D

D

V

V

V

V

L

L

D

D

E

E

R

R

F

F

G

A

G

G

P

P

E

E

Y
|
Y

G

G

L

L

P

P

N

N

D

E

G

G

T

T

L

L

E

E

A

A

I

I

R

R

L

L

C

C

A

A

R

R

L

T

E

E

G

G

V

M

L

L

T

T

D

D

P

P

V

V

Y
|
Y

E
|
E

G

G

K

K

S

S

M

M

H

H

G

G

M

M

I

I

D

E

M

M

V

V

R

R

N

N

G

G

E

E

F

F

P

P

E

E

G

G

S

S

R

R

V

V

L

L

Y

Y

A

A

H

H

L
|
L

G
|
G

G
|
G

V

V

P

P

A

A

L

L

N

N

G

G

Y

Y

S

S

F

F

I

I

F

F

R

R

D

D

G

G

active site: K51 (= K51), Y261 (= Y268), Y287 (= Y294)
binding 2-ketobutyric acid: K51 (= K51), G74 (= G74), S78 (= S78), N79 (= N79), Q80 (= Q80), A153 (= A160), G154 (= G161), Y287 (= Y294)
binding pyridoxal-5'-phosphate: N50 (= N50), K51 (= K51), K54 (= K54), N79 (= N79), C189 (= C196), S190 (= S197), V191 (= V198), T192 (= T199), G193 (= G200), T195 (= T202), Y287 (= Y294), E288 (= E295), L315 (= L322), G316 (= G323), G317 (= G324)

1tzjA Crystal structure of 1-aminocyclopropane-1-carboxylate deaminase complexed with d-vinyl glycine (see paper)
93% identity, 100% coverage: 1:338/338 of query aligns to 1:331/331 of 1tzjA

query
sites
1tzjA

M

M

N

N

L

L

Q

Q

R

R

F

F

P

P

R

R

Y

Y

P

P

L

L

T

T

F

F

G

G

P

P

T

T

P

P

I

I

Q

Q

P

P

L

L

K

A

R

R

L

L

S

S

D

K

H

H

L

L

G

G

G

G

K

K

V

V

H

H

L

L

Y

Y

A

A

K

K

R

R

E

E

D

D

C

C

N

N

S

S

G

G

F

L

A

A

F

F

G

G

G

G

N
|
N

K
|
K

T

T

R

R

K
|
K

L

L

E

E

Y

Y

L

L

I

I

P

P

E

E

A

A

L

L

A

A

Q

Q

G

G

C

C

D

D

T

T

L

L

V

V

S

S

I

I

G
|
G

G

G

I

I

Q

Q

S
|
S

N
|
N

Q
|
Q

T

T

R

R

Q

Q

V

V

A

A

A

A

V

V

A

A

A

A

H

H

L

L

G

G

M

M

K

K

C

C

V

V

L

L

V

V

Q

Q

E

E

N

N

W

W

V

V

N

N

Y

Y

S

S

D

D

A

A

V

V

Y

Y

D

D

R

R

V

V

G

G

N

N

I

I

Q

Q

M

M

S

S

R

R

I

I

L

L

G

G

A

A

D

D

V

V

R

R

L

L

V

V

P

P

D

D

G

-

F

-

D

-

I

-

G

-

F

-

R

-

K

R

S

S

W

W

E

E

D

D

A

A

L

L

E

E

S

S

V

V

R

R

A

A

A

A

G

G

G

G

K

K

P

P

Y

Y

A

A

I

I

P

P

A
|
A

G
|
G

C

C

S

S

D

D

H

H

P

P

L

L

G

G

G

G

L

L

G

G

F

F

V

V

G

G

F

F

A

A

E

E

E

E

V

V

R

R

Q

A

Q

Q

E

E

A

A

E

E

L

L

G

G

F

F

K

K

F

F

D

D

Y

Y

I

V

V

V

V

V

C
|
C

S
|
S

V
|
V

T
|
T

G
|
G

S

S

T
|
T

Q

Q

A

A

G

G

M

M

V

V

V

V

G

G

F

F

A

A

A

A

D

D

G

G

R

R

A

A

D

D

R

R

V

V

I

I

G

G

I

V

D

D

A

A

S

S

A

A

K

K

P

P

A

A

Q

Q

T

T

R

R

E

E

Q

Q

I

I

T

T

R

R

I

I

A

A

S

R

R

Q

T

T

A

A

E

E

K

K

V

V

G

G

L

L

G

E

R

R

D

D

I

I

T

M

A

R

N

A

D

D

V

V

V

V

L

L

D

D

E

E

R

R

F

F

G

A

G

G

P

P

E

E

Y
|
Y

G

G

L

L

P

P

N

N

D

E

G

G

T

T

L

L

E

E

A

A

I

I

R

R

L

L

C

C

A

A

R

R

L

T

E

E

G

G

V

M

L

L

T

T

D

D

P

P

V

V

Y
|
Y

E
|
E

G

G

K

K

S

S

M

M

H

H

G

G

M

M

I

I

D

E

M

M

V

V

R

R

N

N

G

G

E

E

F

F

P

P

E

E

G

G

S

S

R

R

V

V

L

L

Y

Y

A

A

H

H

L
|
L

G
|
G

G
|
G

V

V

P

P

A

A

L

L

N

N

G

G

Y

Y

S

S

F

F

I

I

F

F

R

R

D

D

G

G

active site: K51 (= K51), Y261 (= Y268), Y287 (= Y294)
binding d-vinylglycine: G74 (= G74), S78 (= S78), N79 (= N79), Q80 (= Q80), A153 (= A160), G154 (= G161), Y287 (= Y294)
binding pyridoxal-5'-phosphate: N50 (= N50), K51 (= K51), K54 (= K54), N79 (= N79), C189 (= C196), S190 (= S197), V191 (= V198), T192 (= T199), G193 (= G200), T195 (= T202), Y287 (= Y294), E288 (= E295), L315 (= L322), G316 (= G323), G317 (= G324)

1tz2A Crystal structure of 1-aminocyclopropane-1-carboyxlate deaminase complexed with acc (see paper)
93% identity, 100% coverage: 1:338/338 of query aligns to 1:331/331 of 1tz2A

query
sites
1tz2A

M

M

N

N

L

L

Q

Q

R

R

F

F

P

P

R

R

Y

Y

P

P

L

L

T

T

F

F

G

G

P

P

T

T

P

P

I

I

Q

Q

P

P

L

L

K

A

R

R

L

L

S

S

D

K

H

H

L

L

G

G

G

G

K

K

V

V

H

H

L

L

Y

Y

A

A

K

K

R

R

E

E

D

D

C

C

N

N

S

S

G

G

F

L

A

A

F

F

G

G

G

G

N
|
N

K
|
K

T

T

R

R

K
|
K

L

L

E

E

Y

Y

L

L

I

I

P

P

E

E

A

A

L

L

A

A

Q

Q

G

G

C

C

D

D

T

T

L

L

V

V

S

S

I

I

G

G

G

G

I

I

Q

Q

S
|
S

N
|
N

Q

Q

T

T

R

R

Q

Q

V

V

A

A

A

A

V

V

A

A

A

A

H

H

L

L

G

G

M

M

K

K

C

C

V

V

L

L

V

V

Q

Q

E

E

N

N

W

W

V

V

N

N

Y

Y

S

S

D

D

A

A

V

V

Y

Y

D

D

R

R

V

V

G

G

N

N

I

I

Q

Q

M

M

S

S

R

R

I

I

L

L

G

G

A

A

D

D

V

V

R

R

L

L

V

V

P

P

D

D

G

-

F

-

D

-

I

-

G

-

F

-

R

-

K

R

S

S

W

W

E

E

D

D

A

A

L

L

E

E

S

S

V

V

R

R

A

A

A

A

G

G

G

G

K

K

P

P

Y

Y

A

A

I

I

P

P

A

A

G

G

C

C

S

S

D

D

H

H

P

P

L

L

G

G

G

G

L

L

G

G

F

F

V

V

G

G

F

F

A

A

E

E

E

E

V

V

R

R

Q

A

Q

Q

E

E

A

A

E

E

L

L

G

G

F

F

K

K

F

F

D

D

Y

Y

I

V

V

V

V

V

C
|
C

S
|
S

V
|
V

T
|
T

G
|
G

S

S

T
|
T

Q

Q

A

A

G

G

M

M

V

V

V

V

G

G

F

F

A

A

A

A

D

D

G

G

R

R

A

A

D

D

R

R

V

V

I

I

G

G

I

V

D

D

A

A

S

S

A

A

K

K

P

P

A

A

Q

Q

T

T

R

R

E

E

Q

Q

I

I

T

T

R

R

I

I

A

A

S

R

R

Q

T

T

A

A

E

E

K

K

V

V

G

G

L

L

G

E

R

R

D

D

I

I

T

M

A

R

N

A

D

D

V

V

V

V

L

L

D

D

E

E

R

R

F

F

G

A

G

G

P

P

E

E

Y
|
Y

G

G

L

L

P

P

N

N

D

E

G

G

T

T

L

L

E

E

A

A

I

I

R

R

L

L

C

C

A

A

R

R

L

T

E

E

G

G

V

M

L

L

T

T

D

D

P

P

V

V

Y
|
Y

E
|
E

G

G

K

K

S

S

M

M

H

H

G

G

M

M

I

I

D

E

M

M

V

V

R

R

N

N

G

G

E

E

F

F

P

P

E

E

G

G

S

S

R

R

V

V

L

L

Y

Y

A

A

H

H

L
|
L

G
|
G

G
|
G

V

V

P

P

A

A

L

L

N

N

G

G

Y

Y

S

S

F

F

I

I

F

F

R

R

D

D

G

G

active site: K51 (= K51), Y261 (= Y268), Y287 (= Y294)
binding 1-aminocyclopropanecarboxylic acid: K51 (= K51), S78 (= S78), Y287 (= Y294)
binding pyridoxal-5'-phosphate: N50 (= N50), K51 (= K51), K54 (= K54), N79 (= N79), C189 (= C196), S190 (= S197), V191 (= V198), T192 (= T199), G193 (= G200), T195 (= T202), Y287 (= Y294), E288 (= E295), L315 (= L322), G316 (= G323), G317 (= G324)

Q5PWZ8 1-aminocyclopropane-1-carboxylate deaminase; ACC deaminase; ACCD; EC 3.5.99.7 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see paper)
83% identity, 100% coverage: 1:338/338 of query aligns to 1:338/338 of Q5PWZ8

query
sites
Q5PWZ8

M

M

N

N

L

L

Q

N

R

R

F

F

P

E

R

R

Y

Y

P

P

L

L

T

T

F

F

G

G

P

P

T

S

P

P

I

I

Q

T

P

P

L

L

K

K

R

R

L

L

S

S

D

E

H

H

L

L

G

G

G

G

K

K

V

V

H

E

L

L

Y

Y

A

A

K

K

R

R

E

E

D

D

C

C

N

N

S

S

G
|
G

F

L

A

A

F

F

G

G

G

G

N

N

K

K

T

T

R

R

K

K

L

L

E

E

Y

Y

L

L

I

I

P

P

E

E

A

A

L

I

A

E

Q

Q

G

G

C

C

D

D

T

T

L

L

V

V

S

S

I

I

G

G

G

G

I

I

Q

Q

S

S

N

N

Q

Q

T

T

R

R

Q

Q

V

V

A

A

A

A

V

V

A

A

A

A

H

H

L

L

G

G

M

M

K

K

C

C

V

V

L

L

V

V

Q

Q

E

E

N

N

W

W

V

V

N

N

Y

Y

S

S

D

D

A

A

V

V

Y

Y

D

D

R

R

V

V

G

G

N

N

I

I

Q

E

M

M

S

S

R

R

I

I

L

M

G

G

A

A

D

D

V

V

R

R

L

L

V

D

P

A

D

A

G

G

F

F

D

D

I

I

G

G

F

I

R

R

K

P

S

S

W

W

E

E

D

K

A

A

L

M

E

S

S

D

V

V

R

V

A

E

A

R

G

G

G

G

K

K

P

P

Y

F

A

P

I

I

P

P

A

A

G

G

C

C

S

S

D

E

H

H

P

P

L

Y

G

G

G

G

L

L

G

G

F

F

V

V

G

G

F

F

A

A

E

E

E

E

V

V

R

R

Q

Q

Q

Q

E

E

A

K

E

E

L

L

G

G

F

F

K

K

F

F

D

D

Y

Y

I

I

V

V

V

V

C

C

S

S

V

V

T

T

G

G

S

S

T

T

Q

Q

A

A

G

G

M

M

V

V

V

V

G

G

F

F

A

A

A

A

D

D

G

G

R

R

A

S

D

K

R

N

V

V

I

I

G

G

I

V

D

D

A

A

S

S

A

A

K

K

P

P

A

E

Q

Q

T

T

R

K

E

A

Q

Q

I

I

T

L

R

R

I

I

A

A

S

R

R

H

T

T

A

A

E

E

K

L

V

V

G

E

L

L

G

G

R

R

D

E

I

I

T

T

A

E

N

E

D

D

V

V

V

V

L

L

D

D

E

T

R

R

F

F

G

A

G

Y

P

P

E

E

Y

Y

G

G

L

L

P

P

N

N

D

E

G

G

T

T

L

L

E

E

A

A

I

I

R

R

L

L

C

C

A

G

R

S

L

L

E

E

G

G

V

V

L

L

T

T

D

D

P

P

V

V

Y

Y

E

E

G

G

K

K

S

S

M

M

H

H

G

G

M

M

I

I

D

E

M

M

V

V

R

R

N

R

G

G

E

E

F

F

P

P

E

D

G

G

S

S

R

K

V

V

L

L

Y

Y

A

A

H

H

L

L

G

G

G

G

V

A

P

P

A

A

L

L

N

N

G

A

Y

Y

S

S

F

F

I

L

F

F

R

R

D

N

G

G

G44 (= G44) mutation to D: Loss of activity.

1f2dA 1-aminocyclopropane-1-carboxylate deaminase (see paper)
61% identity, 99% coverage: 3:335/338 of query aligns to 3:336/341 of 1f2dA

query
sites
1f2dA

L

V

Q

A

R

K

F

F

P

A

R

K

Y

Y

P

P

L

L

T

T

F

F

G

G

P

P

T

S

P

P

I

I

Q

S

P

N

L

L

K

N

R

R

L

L

S

S

D

Q

H

H

L

L

G

G

G

S

K

K

V

V

H

N

L

V

Y

Y

A

A

K

K

R

R

E

E

D

D

C

C

N

N

S

S

G

G

F

L

A

A

F

F

G

G

G

G

N
|
N

K
|
K

T

L

R

R

K
|
K

L

L

E

E

Y

Y

L

I

I

V

P

P

E

D

A

I

L

V

A

E

Q

G

G

D

C

Y

D

T

T

H

L

L

V

V

S

S

I

I

G

G

G

G

I

R

Q

Q

S

S

N
|
N

Q

Q

T

T

R

R

Q

M

V

V

A

A

A

A

V

L

A

A

A

A

H

K

L

L

G

G

M

K

K

K

C

C

V

V

L

L

V

I

Q

Q

E

E

N

D

W

W

V

V

N

P

Y

I

S

P

D

E

A

A

-

E

-

K

-

D

V

V

Y

Y

D

N

R

R

V

V

G

G

N

N

I

I

Q

E

M

L

S

S

R

R

I

I

L

M

G

G

A

A

D

D

V

V

R

R

L

V

V

I

P

E

D

D

G

G

F

F

D

D

I

I

G

G

F

M

R

R

K

K

S

S

W

F

E

A

D

N

A

A

L

L

E

Q

S

E

V

L

R

E

A

D

A

A

G

G

G

H

K

K

P

P

Y

Y

A

P

I

I

P

P

A

A

G

G

C

C

S

S

D

E

H

H

P

K

L

Y

G

G

G

G

L

L

G

G

F

F

V

V

G

G

F

F

A

A

E

D

E

E

V

V

R

I

Q

N

Q

Q

E

E

A

V

E

E

L

L

G

G

F

I

K

K

F

F

D

D

Y

K

I

I

V

V

V

V

C

C

S
x
C

V

V

T
|
T

G
|
G

S
|
S

T
|
T

Q

T

A

A

G

G

M

I

V

L

V

A

G

G

F

M

A

A

A

Q

D

Y

G

G

R

R

A

Q

D

D

R

D

V

V

I

I

G

A

I

I

D

D

A

A

S

S

A

F

K

T

P

S

A

E

Q

K

T

T

R

K

E

E

Q

Q

I

T

T

L

R

R

I

I

A

A

S

N

R

N

T

T

A

A

E

K

K

L

V

I

G

G

L

V

G

E

R

H

D

E

I

F

T

-

A

-

N

K

D

D

V

F

V

T

L

L

D

D

E

T

R

R

F

F

G

A

G

Y

P

P

E

C

Y
|
Y

G

G

L

V

P

P

N

N

D

E

G

G

T

T

L

I

E

E

A

A

I

I

R

R

L

T

C

C

A

A

R

E

L

Q

E

E

G

G

V

V

L

L

T

T

D

D

P

P

V

V

Y
|
Y

E
|
E

G

G

K

K

S

S

M

M

H

Q

G

G

M

L

I

I

D

A

M

L

V

I

R

K

N

E

G

D

E

Y

F

F

P

K

E

P

G

G

S

A

R

N

V

V

L

L

Y

Y

A

V

H

H

L
|
L

G
|
G

G
|
G

V

A

P

P

A

A

L

L

N

S

G

A

Y

Y

S

S

F

S

I

F

F

F

active site: K51 (= K51), Y269 (= Y268), Y295 (= Y294)
binding pyridoxal-5'-phosphate: N50 (= N50), K51 (= K51), K54 (= K54), N79 (= N79), C200 (≠ S197), T202 (= T199), G203 (= G200), S204 (= S201), T205 (= T202), Y295 (= Y294), E296 (= E295), L323 (= L322), G324 (= G323), G325 (= G324)

Q7M523 1-aminocyclopropane-1-carboxylate deaminase; ACC deaminase; ACCD; EC 3.5.99.7 from Cyberlindnera saturnus (Yeast) (Williopsis saturnus) (see paper)
61% identity, 99% coverage: 3:335/338 of query aligns to 3:336/341 of Q7M523

query
sites
Q7M523

L

V

Q

A

R

K

F

F

P

A

R

K

Y

Y

P

P

L

L

T

T

F

F

G

G

P

P

T

S

P

P

I

I

Q

S

P

N

L

L

K

N

R

R

L

L

S

S

D

Q

H

H

L

L

G

G

G

S

K

K

V

V

H

N

L

V

Y

Y

A

A

K

K

R

R

E

E

D

D

C

C

N

N

S

S

G

G

F

L

A

A

F

F

G

G

G

G

N

N

K

K

T

L

R

R

K

K

L

L

E

E

Y

Y

L

I

I

V

P

P

E

D

A

I

L

V

A

E

Q

G

G

D

C

Y

D

T

T

H

L

L

V

V

S

S

I

I

G

G

G

G

I

R

Q

Q

S

S

N

N

Q

Q

T

T

R

R

Q

M

V

V

A

A

A

A

V

L

A

A

A

A

H

K

L

L

G

G

M

K

K

K

C

C

V

V

L

L

V

I

Q

Q

E

E

N

D

W

W

V

V

N

P

Y

I

S

P

D

E

A

A

-

E

-

K

-

D

V

V

Y

Y

D

N

R

R

V

V

G

G

N

N

I

I

Q

E

M

L

S

S

R

R

I

I

L

M

G

G

A

A

D

D

V

V

R

R

L

V

V

I

P

E

D

D

G

G

F

F

D

D

I

I

G

G

F

M

R

R

K

K

S

S

W

F

E

A

D

N

A

A

L

L

E

Q

S

E

V

L

R

E

A

D

A

A

G

G

G

H

K

K

P

P

Y

Y

A

P

I

I

P

P

A

A

G

G

C

C

S

S

D

E

H

H

P

K

L

Y

G

G

G

G

L

L

G

G

F

F

V

V

G

G

F

F

A

A

E

D

E

E

V

V

R

I

Q

N

Q

Q

E

E

A

V

E

E

L

L

G

G

F

I

K

K

F

F

D

D

Y

K

I

I

V

V

V

V

C

C

S

C

V

V

T

T

G

G

S

S

T

T

Q

T

A

A

G

G

M

I

V

L

V

A

G

G

F

M

A

A

A

Q

D

Y

G

G

R

R

A

Q

D

D

R

D

V

V

I

I

G

A

I

I

D

D

A

A

S

S

A

F

K

T

P

S

A

E

Q

K

T

T

R

K

E

E

Q

Q

I

T

T

L

R

R

I

I

A

A

S

N

R

N

T

T

A

A

E

K

K

L

V

I

G

G

L

V

G

E

R

H

D

E

I

F

T

-

A

-

N

K

D

D

V

F

V

T

L

L

D

D

E

T

R

R

F

F

G

A

G

Y

P

P

E

C

Y

Y

G

G

L

V

P

P

N

N

D

E

G

G

T

T

L

I

E

E

A

A

I

I

R

R

L

T

C

C

A

A

R

E

L

Q

E

E

G

G

V

V

L

L

T

T

D

D

P

P

V

V

Y

Y

E

E

G

G

K

K

S

S

M

M

H

Q

G

G

M

L

I

I

D

A

M

L

V

I

R

K

N

E

G

D

E

Y

F

F

P

K

E

P

G

G

S

A

R

N

V

V

L

L

Y

Y

A

V

H

H

L

L

G

G

G

G

V

A

P

P

A

A

L

L

N

S

G

A

Y

Y

S

S

F

S

I

F

F

F

Sites not aligning to the query:

1 modified: N-acetylserine

1j0eA Acc deaminase mutant reacton intermediate (see paper)
61% identity, 99% coverage: 3:335/338 of query aligns to 3:336/341 of 1j0eA

query
sites
1j0eA

L

V

Q

A

R

K

F

F

P

A

R

K

Y

Y

P

P

L

L

T

T

F

F

G

G

P

P

T

S

P

P

I

I

Q

S

P

N

L

L

K

N

R

R

L

L

S

S

D

Q

H

H

L

L

G

G

G

S

K

K

V

V

H

N

L

V

Y

Y

A

A

K

K

R

R

E

E

D

D

C

C

N

N

S

S

G

G

F

L

A

A

F

F

G

G

G

G

N
|
N

K
|
K

T

L

R

R

K
|
K

L

L

E

E

Y

Y

L

I

I

V

P

P

E

D

A

I

L

V

A

E

Q

G

G

D

C

Y

D

T

T

H

L

L

V

V

S

S

I

I

G

G

G

G

I

R

Q

Q

S
|
S

N
|
N

Q
|
Q

T

T

R

R

Q

M

V

V

A

A

A

A

V

L

A

A

A

A

H

K

L

L

G

G

M

K

K

K

C

C

V

V

L

L

V

I

Q

Q

E

E

N

D

W

W

V

V

N

P

Y

I

S

P

D

E

A

A

-

E

-

K

-

D

V

V

Y

Y

D

N

R

R

V

V

G

G

N

N

I

I

Q

E

M

L

S

S

R

R

I

I

L

M

G

G

A

A

D

D

V

V

R

R

L

V

V

I

P

E

D

D

G

G

F

F

D

D

I

I

G

G

F

M

R

R

K

K

S

S

W

F

E

A

D

N

A

A

L

L

E

Q

S

E

V

L

R

E

A

D

A

A

G

G

G

H

K

K

P

P

Y

Y

A

P

I

I

P

P

A

A

G

G

C

C

S

S

D

E

H

H

P

K

L

Y

G

G

G

G

L

L

G

G

F

F

V

V

G

G

F

F

A

A

E

D

E

E

V

V

R

I

Q

N

Q

Q

E

E

A

V

E

E

L

L

G

G

F

I

K

K

F

F

D

D

Y

K

I

I

V

V

V

V

C
|
C

S

C

V

V

T
|
T

G
|
G

S

S

T
|
T

Q

T

A

A

G

G

M

I

V

L

V

A

G

G

F

M

A

A

A

Q

D

Y

G

G

R

R

A

Q

D

D

R

D

V

V

I

I

G

A

I

I

D

D

A

A

S

S

A

F

K

T

P

S

A

E

Q

K

T

T

R

K

E

E

Q

Q

I

T

T

L

R

R

I

I

A

A

S

N

R

N

T

T

A

A

E

K

K

L

V

I

G

G

L

V

G

E

R

H

D

E

I

F

T

-

A

-

N

K

D

D

V

F

V

T

L

L

D

D

E

T

R

R

F

F

G

A

G

Y

P

P

E

C

Y

Y

G

G

L

V

P

P

N

N

D

E

G

G

T

T

L

I

E

E

A

A

I

I

R

R

L

T

C

C

A

A

R

E

L

Q

E

E

G

G

V

V

L

L

T

T

D

D

P

P

V

V

Y
x
F

E
|
E

G

G

K

K

S

S

M

M

H

Q

G

G

M

L

I

I

D

A

M

L

V

I

R

K

N

E

G

D

E

Y

F

F

P

K

E

P

G

G

S

A

R

N

V

V

L

L

Y

Y

A

V

H

H

L

L

G

G

G

G

V

A

P

P

A

A

L

L

N

S

G

A

Y

Y

S

S

F

S

I

F

F

F

binding 1-aminocyclopropanecarboxylic acid: S78 (= S78), N79 (= N79), Q80 (= Q80), F295 (≠ Y294)
binding pyridoxal-5'-phosphate: N50 (= N50), K51 (= K51), K54 (= K54), N79 (= N79), C199 (= C196), T202 (= T199), G203 (= G200), T205 (= T202), F295 (≠ Y294), E296 (= E295)

1j0dA Acc deaminase mutant complexed with acc (see paper)
61% identity, 99% coverage: 3:335/338 of query aligns to 3:336/341 of 1j0dA

query
sites
1j0dA

L

V

Q

A

R

K

F

F

P

A

R

K

Y

Y

P

P

L

L

T

T

F

F

G

G

P

P

T

S

P

P

I

I

Q

S

P

N

L

L

K

N

R

R

L

L

S

S

D

Q

H

H

L

L

G

G

G

S

K

K

V

V

H

N

L

V

Y

Y

A

A

K

K

R

R

E

E

D

D

C

C

N

N

S

S

G

G

F

L

A

A

F

F

G

G

G

G

N

N

K

T

T

L

R

R

K

K

L

L

E

E

Y

Y

L

I

I

V

P

P

E

D

A

I

L

V

A

E

Q

G

G

D

C

Y

D

T

T

H

L

L

V

V

S

S

I

I

G

G

G

G

I

R

Q

Q

S
|
S

N
|
N

Q
|
Q

T

T

R

R

Q

M

V

V

A

A

A

A

V

L

A

A

A

A

H

K

L

L

G

G

M

K

K

K

C

C

V

V

L

L

V

I

Q

Q

E

E

N

D

W

W

V

V

N

P

Y

I

S

P

D

E

A

A

-

E

-

K

-

D

V

V

Y

Y

D

N

R

R

V

V

G

G

N

N

I

I

Q

E

M

L

S

S

R

R

I

I

L

M

G

G

A

A

D

D

V

V

R

R

L

V

V

I

P

E

D

D

G

G

F

F

D

D

I

I

G

G

F

M

R

R

K

K

S

S

W

F

E

A

D

N

A

A

L

L

E

Q

S

E

V

L

R

E

A

D

A

A

G

G

G

H

K

K

P

P

Y

Y

A

P

I

I

P

P

A
|
A

G
|
G

C

C

S

S

D

E

H

H

P

K

L

Y

G

G

G

G

L

L

G

G

F

F

V

V

G

G

F

F

A

A

E

D

E

E

V

V

R

I

Q

N

Q

Q

E

E

A

V

E

E

L

L

G

G

F

I

K

K

F

F

D

D

Y

K

I

I

V

V

V

V

C

C

S
x
C

V
|
V

T
|
T

G
|
G

S
|
S

T
|
T

Q

T

A

A

G

G

M

I

V

L

V

A

G

G

F

M

A

A

A

Q

D

Y

G

G

R

R

A

Q

D

D

R

D

V

V

I

I

G

A

I

I

D

D

A

A

S

S

A

F

K

T

P

S

A

E

Q

K

T

T

R

K

E

E

Q

Q

I

T

T

L

R

R

I

I

A

A

S

N

R

N

T

T

A

A

E

K

K

L

V

I

G

G

L

V

G

E

R

H

D

E

I

F

T

-

A

-

N

K

D

D

V

F

V

T

L

L

D

D

E

T

R

R

F

F

G

A

G

Y

P

P

E

C

Y

Y

G

G

L

V

P

P

N

N

D

E

G

G

T

T

L

I

E

E

A

A

I

I

R

R

L

T

C

C

A

A

R

E

L

Q

E

E

G

G

V

V

L

L

T

T

D

D

P

P

V

V

Y
|
Y

E
|
E

G

G

K

K

S

S

M

M

H

Q

G

G

M

L

I

I

D

A

M

L

V

I

R

K

N

E

G

D

E

Y

F

F

P

K

E

P

G

G

S

A

R

N

V

V

L

L

Y

Y

A

V

H

H

L

L

G

G

G

G

V

A

P

P

A

A

L

L

N

S

G

A

Y

Y

S

S

F

S

I

F

F

F

binding n-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-y-lmethyl]-1-amino-cyclopropanecarboxylic acid: S78 (= S78), N79 (= N79), Q80 (= Q80), A163 (= A160), G164 (= G161), C200 (≠ S197), V201 (= V198), T202 (= T199), G203 (= G200), S204 (= S201), T205 (= T202), Y295 (= Y294), E296 (= E295)

4d8uH Crystal structure of d-cysteine desulfhydrase from salmonella typhimurium at 3.3 a in monoclinic space group with 8 subunits in the asymmetric unit (see paper)
37% identity, 98% coverage: 2:331/338 of query aligns to 8:327/331 of 4d8uH

query
sites
4d8uH

N

H

L

L

Q

T

R

R

F

F

P

P

R

R

Y

L

P

E

L

F

T

I

F

G

G

A

P

P

T

T

P

P

I

L

Q

E

P

Y

L

L

K

P

R

R

L

L

S

S

D

D

H

Y

L

L

G

G

G

R

K

E

V

I

H

-

L

-

Y

Y

A

I

K

K

R

R

E

D

D

D

C

V

N

T

S

P

G

-

F

I

A

A

F

M

G

G

G

G

N

N

K
|
K

T

L

R

R

K

K

L

L

E

E

Y

F

L

L

I

V

P

A

E

D

A

A

L

L

A

R

Q

E

G

G

C

A

D

D

T

T

L

L

V

I

S

T

I

A

G

G

G

A

I

I

Q

Q

S
|
S

N

N

Q
x
H

T

V

R

R

Q

Q

V

T

A

A

A

A

V

V

A

A

A

A

H

K

L

L

G

G

M

L

K

H

C

C

V

V

L

A

V

L

Q

L

E

E

N

N

W

P

V

I

N

G

Y

T

S

T

D

A

A

E

V

N

Y

Y

D

L

R

T

V

N

G

G

N

N

I

R

Q

L

M

L

S

L

R

D

I

L

L

F

G

N

A

T

D

Q

V

I

R

E

L

M

V

C

-

D

-

A

-

L

-

T

-

D

P

P

D

D

G

A

F

-

D

-

I

-

G

-

F

-

R

-

K

-

S

Q

W

L

E

Q

D

T

A

L

L

A

E

T

S

R

V

I

R

E

A

A

A

Q

G

G

G

F

K

R

P

P

Y

Y

A

V

I

I

P

P

A

V

G

G

C

G

S

S

D

S

H

-

P

A

L

L

G

G

G

A

L

M

G

G

F

Y

V

V

G

E

F

S

A

A

E

L

E

E

V

I

R

A

Q

Q

Q

Q

E

C

A

E

E

E

L

V

G

-

F

V

K

G

F

L

D

S

Y

S

I

V

V

V

V

V

C

A

S

S

V

G

T

S

G

A

S

G

T

T

Q

H

A

A

G

G

M

L

V

A

V

V

G

G

F

L

A

E

A

H

D

L

G

M

R

P

A

D

D

V

R

E

V

L

I

I

G

G

I

V

D

T

A

V

S

S

A

R

K

S

P

V

A

A

Q

E

T

Q

R

K

E

P

Q

K

I

V

T

I

R

A

I

L

A

Q

S

Q

R

A

T

I

A

A

E

G

K

Q

V

L

G

A

L

L

G

-

R

-

D

-

I

T

T

A

A

T

N

A

D

D

V

I

V

H

L

L

D

W

E

D

R

D

F

Y

G

F

G

A

P

P

E

G

Y
|
Y

G

G

L

V

P

P

N

N

D

D

G

A

T

G

L

M

E

E

A

A

I

V

R

K

L

L

C

L

A

A

R

S

L

L

E

E

G

G

V

V

L

L

T

L

D

D

P

P

V

V

Y
|
Y

E

T

G

G

K

K

S

A

M

M

H

A

G

G

M

L

I

I

D

D

M

G

V

I

R

S

N

Q

G

K

E

R

F

F

P

N

E

D

G

D

S

G

R

P

V

I

L

L

Y

F

A

I

H

H

L

T

G

G

G

G

V

A

P

P

A

A

L

L

N

F

G

A

Y

Y

active site: K54 (= K51), Y264 (= Y268), Y290 (= Y294)
binding phosphate ion: K54 (= K51), S81 (= S78), H83 (≠ Q80), Y290 (= Y294)

4d9fA D-cysteine desulfhydrase from salmonella typhimurium complexed with d- cycloserine (dcs) (see paper)
37% identity, 98% coverage: 2:331/338 of query aligns to 5:324/328 of 4d9fA

query
sites
4d9fA

N

H

L

L

Q

T

R

R

F

F

P

P

R

R

Y

L

P

E

L

F

T

I

F

G

G

A

P

P

T

T

P

P

I

L

Q

E

P

Y

L

L

K

P

R

R

L

L

S

S

D

D

H

Y

L

L

G

G

G

R

K

E

V

I

H

-

L

-

Y

Y

A

I

K

K

R

R

E

D

D

D

C

V

N

T

S

P

G

-

F

I

A

A

F

M

G

G

G

G

N
|
N

K
|
K

T

L

R

R

K
|
K

L

L

E

E

Y

F

L

L

I

V

P

A

E

D

A

A

L

L

A

R

Q

E

G

G

C

A

D

D

T

T

L

L

V

I

S

T

I

A

G

G

G

A

I

I

Q

Q

S

S

N
|
N

Q

H

T

V

R

R

Q

Q

V

T

A

A

A

A

V

V

A

A

A

A

H

K

L

L

G

G

M

L

K

H

C

C

V

V

L

A

V

L

Q

L

E

E

N

N

W

P

V

I

N

G

Y

T

S

T

D

A

A

E

V

N

Y

Y

D

L

R

T

V

N

G

G

N

N

I

R

Q

L

M

L

S

L

R

D

I

L

L

F

G

N

A

T

D

Q

V

I

R

E

L

M

V

C

-

D

-

A

-

L

-

T

-

D

P

P

D

D

G

A

F

-

D

-

I

-

G

-

F

-

R

-

K

-

S

Q

W

L

E

Q

D

T

A

L

L

A

E

T

S

R

V

I

R

E

A

A

A

Q

G

G

G

F

K

R

P

P

Y

Y

A

V

I

I

P

P

A

V

G

G

C

G

S

S

D

S

H

-

P

A

L

L

G

G

G

A

L

M

G

G

F

Y

V

V

G

E

F

S

A

A

E

L

E

E

V

I

R

A

Q

Q

Q

Q

E

C

A

E

E

E

L

V

G

-

F

V

K

G

F

L

D

S

Y

S

I

V

V

V

V

V

C

A

S

S

V
x
G

T
x
S

G
x
A

S

G

T
|
T

Q

H

A

A

G

G

M

L

V

A

V

V

G

G

F

L

A

E

A

H

D

L

G

M

R

P

A

D

D

V

R

E

V

L

I

I

G

G

I

V

D

T

A

V

S

S

A

R

K

S

P

V

A

A

Q

E

T

Q

R

K

E

P

Q

K

I

V

T

I

R

A

I

L

A

Q

S

Q

R

A

T

I

A

A

E

G

K

Q

V

L

G

A

L

L

G

-

R

-

D

-

I

T

T

A

A

T

N

A

D

D

V

I

V

H

L

L

D

W

E

D

R

D

F

Y

G

F

G

A

P

P

E

G

Y
|
Y

G

G

L

V

P

P

N

N

D

D

G

A

T

G

L

M

E

E

A

A

I

V

R

K

L

L

C

L

A

A

R

S

L

L

E

E

G

G

V

V

L

L

T

L

D

D

P

P

V

V

Y
|
Y

E

T

G

G

K

K

S

A

M

M

H

A

G

G

M

L

I

I

D

D

M

G

V

I

R

S

N

Q

G

K

E

R

F

F

P

N

E

D

G

D

S

G

R

P

V

I

L

L

Y

F

A

I

H

H

L
x
T

G
|
G

G
|
G

V

A

P

P

A

A

L

L

N

F

G

A

Y

Y

active site: K51 (= K51), Y261 (= Y268), Y287 (= Y294)
binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: N50 (= N50), K51 (= K51), K54 (= K54), N79 (= N79), G194 (≠ V198), S195 (≠ T199), A196 (≠ G200), T198 (= T202), Y287 (= Y294), T315 (≠ L322), G316 (= G323), G317 (= G324)

4d9eA D-cysteine desulfhydrase from salmonella typhimurium complexed with l- cycloserine (lcs) (see paper)
37% identity, 98% coverage: 2:331/338 of query aligns to 5:324/328 of 4d9eA

query
sites
4d9eA

N

H

L

L

Q

T

R

R

F

F

P

P

R

R

Y

L

P

E

L

F

T

I

F

G

G

A

P

P

T

T

P

P

I

L

Q

E

P

Y

L

L

K

P

R

R

L

L

S

S

D

D

H

Y

L

L

G

G

G

R

K

E

V

I

H

-

L

-

Y

Y

A

I

K

K

R

R

E

D

D

D

C

V

N

T

S

P

G

-

F

I

A

A

F

M

G

G

G

G

N
|
N

K
|
K

T

L

R

R

K

K

L

L

E

E

Y

F

L

L

I

V

P

A

E

D

A

A

L

L

A

R

Q

E

G

G

C

A

D

D

T

T

L

L

V

I

S

T

I

A

G

G

G

A

I

I

Q

Q

S

S

N
|
N

Q

H

T

V

R

R

Q

Q

V

T

A

A

A

A

V

V

A

A

A

A

H

K

L

L

G

G

M

L

K

H

C

C

V

V

L

A

V

L

Q

L

E

E

N

N

W

P

V

I

N

G

Y

T

S

T

D

A

A

E

V

N

Y

Y

D

L

R

T

V

N

G

G

N

N

I

R

Q

L

M

L

S

L

R

D

I

L

L

F

G

N

A

T

D

Q

V

I

R

E

L

M

V

C

-

D

-

A

-

L

-

T

-

D

P

P

D

D

G

A

F

-

D

-

I

-

G

-

F

-

R

-

K

-

S

Q

W

L

E

Q

D

T

A

L

L

A

E

T

S

R

V

I

R

E

A

A

A

Q

G

G

G

F

K

R

P

P

Y

Y

A

V

I

I

P

P

A

V

G

G

C

G

S

S

D

S

H

-

P

A

L

L

G

G

G

A

L

M

G

G

F

Y

V

V

G

E

F

S

A

A

E

L

E

E

V

I

R

A

Q

Q

Q

Q

E

C

A

E

E

E

L

V

G

-

F

V

K

G

F

L

D

S

Y

S

I

V

V

V

V

V

C

A

S

S

V
x
G

T
x
S

G
x
A

S

G

T
|
T

Q

H

A

A

G

G

M

L

V

A

V

V

G

G

F

L

A

E

A

H

D

L

G

M

R

P

A

D

D

V

R

E

V

L

I

I

G

G

I

V

D

T

A

V

S

S

A

R

K

S

P

V

A

A

Q

E

T

Q

R

K

E

P

Q

K

I

V

T

I

R

A

I

L

A

Q

S

Q

R

A

T

I

A

A

E

G

K

Q

V

L

G

A

L

L

G

-

R

-

D

-

I

T

T

A

A

T

N

A

D

D

V

I

V

H

L

L

D

W

E

D

R

D

F

Y

G

F

G

A

P

P

E

G

Y
|
Y

G

G

L

V

P

P

N

N

D

D

G

A

T

G

L

M

E

E

A

A

I

V

R

K

L

L

C

L

A

A

R

S

L

L

E

E

G

G

V

V

L

L

T

L

D

D

P

P

V

V

Y
|
Y

E

T

G

G

K

K

S

A

M

M

H

A

G

G

M

L

I

I

D

D

M

G

V

I

R

S

N

Q

G

K

E

R

F

F

P

N

E

D

G

D

S

G

R

P

V

I

L

L

Y

F

A

I

H

H

L
x
T

G
|
G

G
|
G

V

A

P

P

A

A

L

L

N

F

G

A

Y

Y

active site: K51 (= K51), Y261 (= Y268), Y287 (= Y294)
binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: N50 (= N50), K51 (= K51), N79 (= N79), G194 (≠ V198),